CSPI02G14340 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI02G14340
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	EF hand calcium-binding family protein
Location	Chr2 : 14164740 .. 14166059 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCTAAACTATAGAATAAAAAAGTATTATTTTTAAAAAATATAGGAAAAATAAATTTATATAGGAGGGATATAAATAGAGGGTTGATGGTTATACATTAATTGTGATTGGCATATAGGAAGAGATGTCAAGCTTATTGAAGTTATTCAGAAGCTGTTCTGGAAAGCGACCATCCAAAAACACTTCCTTTTCCATACCAATCAAAGAAAGGTCCAATTCTTCCTCACCCTCTTCAAAGCAATTCCGCCCAAACTTGGAGGAGATCAAATGGGTATGAACAATTCTTTTTTTGCATGAATTATTTCAAATCTTTTTATGAAAAAAACATTGAATTATTTCAAATGTTCATAAGCAATAAAGTTGCTGAGAAAGTTATGAACTGGAACTCTCTATAACAACATGCTGTGCTACGTACGTTCGTAGTAATGATCTCTTTTGGATTTAATAGGCGTTTGAGAAGTTTGACTCGAACAAGGATGGAAAAATCTCATTTGAGGAGTACAAGGAAGCTCACCGAGGCTTGGCTGGAAGCAAAGAGATCACAGACGCAGAGGCAGAGAAGTCATTCAAGCTTGTGGATGTAGATGGCGATGGATTCGTGGATTTGAAGGAGTTCGTGGAGCTGTACACGATGAGCAGCGGGGAAGTGAAGGTGGGCGACATCGAAAGTGCCTTCAAAGTTTATGATTCCAATGGAGATGGGAAGATAAGTGCAGAGGAGGTGATGGGGATCATGAAGATATTGGGGGAGAACACCACCTTGAAGGCTTGCAAGCAAATGGTGAAGGGTGTGGACATGGATGGGGATGGCTTTATTGATGTCCAAGAGTTTTCTAAGCTCATGGGTAAGTATTCGAGTAAGTTTCAAAATTAAAGAACCTAAAACTACAACTTCGGACGCCCCGTATCCATTTTAATTTTTCTTGAGTGTAACGGTTGATCTCTAGCTTAATTTTCAAAAACAAAAACAAAAAAATAAAATGATTGGAAAACGAGAGCTACACATGTCTCTAGTTTTTAGACAAATGAATGTGGAAAACAAAAATTCTAAGCTATATTTGAAGTTTGTATCCAATAATTAGGAGGATAGTTATATTGTGACAATGTTGGTGATTAATGTGCGACTACTAGTAGTTATTATGCAACGTGTAGATTTGTGATGTAATTTTTGTTAAGTTGTCAACATCTTCAAACGATGAAGGGTTCAATATTGATATAGTGAATTGAAAATCTCTATTATATAAGAAAATTGATGTCAAGATTACTTTTATTGATATTTTCGTCAAGATTTACATTATTGTCAACATTCTTATGATCGGTG

mRNA sequence

ATGTCAAGCTTATTGAAGTTATTCAGAAGCTGTTCTGGAAAGCGACCATCCAAAAACACTTCCTTTTCCATACCAATCAAAGAAAGGTCCAATTCTTCCTCACCCTCTTCAAAGCAATTCCGCCCAAACTTGGAGGAGATCAAATGGGCGTTTGAGAAGTTTGACTCGAACAAGGATGGAAAAATCTCATTTGAGGAGTACAAGGAAGCTCACCGAGGCTTGGCTGGAAGCAAAGAGATCACAGACGCAGAGGCAGAGAAGTCATTCAAGCTTGTGGATGTAGATGGCGATGGATTCGTGGATTTGAAGGAGTTCGTGGAGCTGTACACGATGAGCAGCGGGGAAGTGAAGGTGGGCGACATCGAAAGTGCCTTCAAAGTTTATGATTCCAATGGAGATGGGAAGATAAGTGCAGAGGAGGTGATGGGGATCATGAAGATATTGGGGGAGAACACCACCTTGAAGGCTTGCAAGCAAATGGTGAAGGGTGTGGACATGGATGGGGATGGCTTTATTGATGTCCAAGAGTTTTCTAAGCTCATGGGTAAGTATTCGAGTAAGTTTCAAAATTAA

Coding sequence (CDS)

ATGTCAAGCTTATTGAAGTTATTCAGAAGCTGTTCTGGAAAGCGACCATCCAAAAACACTTCCTTTTCCATACCAATCAAAGAAAGGTCCAATTCTTCCTCACCCTCTTCAAAGCAATTCCGCCCAAACTTGGAGGAGATCAAATGGGCGTTTGAGAAGTTTGACTCGAACAAGGATGGAAAAATCTCATTTGAGGAGTACAAGGAAGCTCACCGAGGCTTGGCTGGAAGCAAAGAGATCACAGACGCAGAGGCAGAGAAGTCATTCAAGCTTGTGGATGTAGATGGCGATGGATTCGTGGATTTGAAGGAGTTCGTGGAGCTGTACACGATGAGCAGCGGGGAAGTGAAGGTGGGCGACATCGAAAGTGCCTTCAAAGTTTATGATTCCAATGGAGATGGGAAGATAAGTGCAGAGGAGGTGATGGGGATCATGAAGATATTGGGGGAGAACACCACCTTGAAGGCTTGCAAGCAAATGGTGAAGGGTGTGGACATGGATGGGGATGGCTTTATTGATGTCCAAGAGTTTTCTAAGCTCATGGGTAAGTATTCGAGTAAGTTTCAAAATTAA

Homology

[Close]

BLAST of CSPI02G14340 vs. Swiss-Prot
Match: CML1_ARATH (Calmodulin-like protein 1 OS=Arabidopsis thaliana GN=CML1 PE=2 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.1e-29
Identity = 77/177 (43.50%), Postives = 113/177 (63.84%), Query Frame = 1


Query: 6   KLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKQF-RPNLEEIKWAFEKFDSNKDGKISF 65
           KL ++   ++PS+  S     ++ S  SSP    F +P++ E++  F +FD +KDGKIS 
Sbjct: 12  KLSKNKMLRKPSRMFSRD---RQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLDKDGKISQ 71

Query: 66  EEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGDIESA 125
            EYK   R L   + I D    K FK VD+DGDGF+D +EF++ Y  S G ++  DI ++
Sbjct: 72  TEYKVVLRALGQERAIEDVP--KIFKAVDLDGDGFIDFREFIDAYKRSGG-IRSSDIRNS 131

Query: 126 FKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKLM 182
           F  +D NGDGKISAEEVM ++  LGE  +L+ C +MV+ VD DGDG ++++EF K+M
Sbjct: 132 FWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKMM 182

[Close]

BLAST of CSPI02G14340 vs. Swiss-Prot
Match: CML24_ARATH (Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2)

HSP 1 Score: 115.2 bits (287), Expect = 8.1e-25
Identity = 61/144 (42.36%), Postives = 93/144 (64.58%), Query Frame = 1


Query: 43  NLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDL 102
           ++++IK  F++FD N DGKIS +E KE  R L  S   +  E     K  D+DG+GF+DL
Sbjct: 14  SMDDIKKVFQRFDKNGDGKISVDELKEVIRAL--SPTASPEETVTMMKQFDLDGNGFIDL 73

Query: 103 KEFVELYTMSSG-----EVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKAC 162
            EFV L+ +  G        V D++ AF++YD +G+G+ISA+E+  +MK LGE  +++ C
Sbjct: 74  DEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDC 133

Query: 163 KQMVKGVDMDGDGFIDVQEFSKLM 182
           K+M+  VD+DGDG ++  EF K+M
Sbjct: 134 KKMISKVDIDGDGCVNFDEFKKMM 155

[Close]

BLAST of CSPI02G14340 vs. Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.1e-24
Identity = 59/142 (41.55%), Postives = 91/142 (64.08%), Query Frame = 1


Query: 43  NLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDL 102
           ++E+IK  F++FD N DGKIS +E K+    L  S   +  E +   K  D+DG+GF+DL
Sbjct: 12  SMEDIKKVFQRFDKNNDGKISIDELKDVIGAL--SPNASQEETKAMMKEFDLDGNGFIDL 71

Query: 103 KEFVELYTMS---SGEVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQ 162
            EFV L+ +S   S    + D++ AF +YD + +G+ISA E+  +MK LGE  +++ C++
Sbjct: 72  DEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQR 131

Query: 163 MVKGVDMDGDGFIDVQEFSKLM 182
           M+  VD DGDG +D +EF K+M
Sbjct: 132 MINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of CSPI02G14340 vs. Swiss-Prot
Match: CML32_ARATH (Probable calcium-binding protein CML32 OS=Arabidopsis thaliana GN=CML32 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 4.4e-23
Identity = 63/143 (44.06%), Postives = 86/143 (60.14%), Query Frame = 1


Query: 51  FEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYT 110
           FE+ D NKDGKIS++E+ EA R  A S  IT  E +  F+ +DVDGD  +D+ E+     
Sbjct: 7   FERVDKNKDGKISWDEFAEAIR--AFSPSITSEEIDNMFREIDVDGDNQIDVAEYASCL- 66

Query: 111 MSSGEVKVGD----IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDM 170
           M  GE    D    ++ AF +YD +GDGKISA E+  ++K LGE  T+  C  MV+ VD 
Sbjct: 67  MLGGEGNKEDEDIVMKEAFDLYDIDGDGKISASEIHVVLKRLGEKQTIAECIAMVRAVDA 126

Query: 171 DGDGFIDVQEFSKLMGKYSSKFQ 190
           DGDGF+  +EF  +M   + K Q
Sbjct: 127 DGDGFVSFEEFKTMMSCNNKKLQ 146

[Close]

BLAST of CSPI02G14340 vs. Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 3.2e-21
Identity = 56/142 (39.44%), Postives = 88/142 (61.97%), Query Frame = 1


Query: 41  RPNLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFV 100
           +  + E++  F+KFD N DGKIS +E       L    E+ + E EK+   +D  GDG++
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLG--HEVPEEELEKAITEIDRKGDGYI 91

Query: 101 DLKEFVELYTMSSGEVKV-GDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQ 160
           + +EFVEL T    +  V  +++ AF VYD +G+G ISAEE+  +++ LG+  ++  C++
Sbjct: 92  NFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRK 151

Query: 161 MVKGVDMDGDGFIDVQEFSKLM 182
           M+ GVD DGDG ID +EF  +M
Sbjct: 152 MIGGVDKDGDGTIDFEEFKIMM 171

[Close]

BLAST of CSPI02G14340 vs. TrEMBL
Match: A0A0A0LMP3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G286450 PE=4 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 2.2e-98
Identity = 189/190 (99.47%), Postives = 189/190 (99.47%), Query Frame = 1


Query: 1   MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKQFRPNLEEIKWAFEKFDSNKDG 60
           MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSK FRPNLEEIKWAFEKFDSNKDG
Sbjct: 1   MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDG 60

Query: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 120
           KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD
Sbjct: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 120

Query: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 180
           IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL
Sbjct: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 180

Query: 181 MGKYSSKFQN 191
           MGKYSSKFQN
Sbjct: 181 MGKYSSKFQN 190

[Close]

BLAST of CSPI02G14340 vs. TrEMBL
Match: W9RW36_9ROSA (Calmodulin-like protein 1 OS=Morus notabilis GN=L484_002706 PE=4 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 4.3e-41
Identity = 85/147 (57.82%), Postives = 113/147 (76.87%), Query Frame = 1


Query: 44  LEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLK 103
           +EE++WAFEKFD+N+DGKIS EEYK A R L   +EITD E  K+F  +D DGDGFVD K
Sbjct: 1   MEELRWAFEKFDTNRDGKISREEYKSALRVL--DREITDGEIAKAFNALDTDGDGFVDFK 60

Query: 104 EFVELYTMSSGEVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKG 163
           EFVE++ M SG VK  DIESAF+++D +GDGKISAEE+  ++K LGE+ +++AC+ MVKG
Sbjct: 61  EFVEMFDMGSGGVKASDIESAFRLFDLDGDGKISAEELSQVLKQLGESCSIRACRNMVKG 120

Query: 164 VDMDGDGFIDVQEFSKLMGKYSSKFQN 191
           VD DGDGFI+++EF+++M        N
Sbjct: 121 VDNDGDGFINIEEFTRMMSTTKKPITN 145

[Close]

BLAST of CSPI02G14340 vs. TrEMBL
Match: I1LHF1_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_11G056500 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 4.5e-38
Identity = 87/172 (50.58%), Postives = 124/172 (72.09%), Query Frame = 1


Query: 10  SCSGKRPSKNTSFSIPIKERSNSSSPSSKQFRPNLEEIKWAFEKFDSNKDGKISFEEYKE 69
           S S K  +K  SF       S  +S   + F+P  EE+KW F+KFD+N+DGK+S EEYK 
Sbjct: 18  SSSRKNVAKKNSFV------SRQTSNEGQSFQPTKEEMKWVFQKFDTNRDGKVSLEEYKA 77

Query: 70  AHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGDIESAFKVYD 129
           A R L   + I +AEA K+F+++D+DGDGF+DL EF+E++    G +K  +I++AF+V+D
Sbjct: 78  AARAL--DRAIGEAEAVKAFRVMDIDGDGFIDLNEFMEMFN-GEGRIKETEIKNAFQVFD 137

Query: 130 SNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKLM 182
            NGDGKISAEE+  ++K LGE+ +L ACK+MVKGVD +GDGFID+ EF+++M
Sbjct: 138 LNGDGKISAEELSHVLKRLGESCSLSACKKMVKGVDGNGDGFIDLNEFTRMM 180

[Close]

BLAST of CSPI02G14340 vs. TrEMBL
Match: A0A0B2SA84_GLYSO (Calmodulin-like protein 1 OS=Glycine soja GN=glysoja_003815 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 4.5e-38
Identity = 87/172 (50.58%), Postives = 124/172 (72.09%), Query Frame = 1


Query: 10  SCSGKRPSKNTSFSIPIKERSNSSSPSSKQFRPNLEEIKWAFEKFDSNKDGKISFEEYKE 69
           S S K  +K  SF       S  +S   + F+P  EE+KW F+KFD+N+DGK+S EEYK 
Sbjct: 18  SSSRKNVAKKNSFV------SRQTSNEGQSFQPTKEEMKWVFQKFDTNRDGKVSLEEYKA 77

Query: 70  AHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGDIESAFKVYD 129
           A R L   + I +AEA K+F+++D+DGDGF+DL EF+E++    G +K  +I++AF+V+D
Sbjct: 78  AARAL--DRAIGEAEAVKAFRVMDIDGDGFIDLNEFMEMFN-GEGRIKETEIKNAFQVFD 137

Query: 130 SNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKLM 182
            NGDGKISAEE+  ++K LGE+ +L ACK+MVKGVD +GDGFID+ EF+++M
Sbjct: 138 LNGDGKISAEELSHVLKRLGESCSLSACKKMVKGVDGNGDGFIDLNEFTRMM 180

[Close]

BLAST of CSPI02G14340 vs. TrEMBL
Match: G7K7Z2_MEDTR (EF hand calcium-binding family protein OS=Medicago truncatula GN=MTR_5g017550 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 4.5e-38
Identity = 94/181 (51.93%), Postives = 124/181 (68.51%), Query Frame = 1


Query: 3   SLLKLFRSCSGK--RPSKNTSFSIPIKERSNSSSPSSKQFRPNLEEIKWAFEKFDSNKDG 62
           S LK   S S K   P KN +     K R  SS+  S  F+P  +E+KW FEKFD+NKDG
Sbjct: 5   SFLKFHGSFSAKPTSPRKNIAQKNSFKLRETSSTEMS--FQPKKDEMKWVFEKFDTNKDG 64

Query: 63  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 122
           KIS EEYK A + L   K I D +A K+F ++D D DGF+D KEF+E++   + ++K  +
Sbjct: 65  KISLEEYKAAAKSL--DKGIGDPDAVKAFNVMDSDKDGFIDFKEFMEMFNGENNKIKEEE 124

Query: 123 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 182
           I+SAF+V+D NGDGKISAEE+  I K LGE+ +L ACK+MVKGVD DGDG ID+ EF+++
Sbjct: 125 IKSAFQVFDINGDGKISAEELSQIFKRLGESCSLSACKKMVKGVDGDGDGLIDLNEFTRM 181

[Close]

BLAST of CSPI02G14340 vs. TAIR10
Match: AT2G15680.1 (AT2G15680.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 131.3 bits (329), Expect = 6.2e-31
Identity = 77/177 (43.50%), Postives = 113/177 (63.84%), Query Frame = 1


Query: 6   KLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKQF-RPNLEEIKWAFEKFDSNKDGKISF 65
           KL ++   ++PS+  S     ++ S  SSP    F +P++ E++  F +FD +KDGKIS 
Sbjct: 12  KLSKNKMLRKPSRMFSRD---RQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLDKDGKISQ 71

Query: 66  EEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGDIESA 125
            EYK   R L   + I D    K FK VD+DGDGF+D +EF++ Y  S G ++  DI ++
Sbjct: 72  TEYKVVLRALGQERAIEDVP--KIFKAVDLDGDGFIDFREFIDAYKRSGG-IRSSDIRNS 131

Query: 126 FKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKLM 182
           F  +D NGDGKISAEEVM ++  LGE  +L+ C +MV+ VD DGDG ++++EF K+M
Sbjct: 132 FWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKMM 182

[Close]

BLAST of CSPI02G14340 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 115.2 bits (287), Expect = 4.6e-26
Identity = 61/144 (42.36%), Postives = 93/144 (64.58%), Query Frame = 1


Query: 43  NLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDL 102
           ++++IK  F++FD N DGKIS +E KE  R L  S   +  E     K  D+DG+GF+DL
Sbjct: 14  SMDDIKKVFQRFDKNGDGKISVDELKEVIRAL--SPTASPEETVTMMKQFDLDGNGFIDL 73

Query: 103 KEFVELYTMSSG-----EVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKAC 162
            EFV L+ +  G        V D++ AF++YD +G+G+ISA+E+  +MK LGE  +++ C
Sbjct: 74  DEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDC 133

Query: 163 KQMVKGVDMDGDGFIDVQEFSKLM 182
           K+M+  VD+DGDG ++  EF K+M
Sbjct: 134 KKMISKVDIDGDGCVNFDEFKKMM 155

[Close]

BLAST of CSPI02G14340 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 113.2 bits (282), Expect = 1.7e-25
Identity = 59/142 (41.55%), Postives = 91/142 (64.08%), Query Frame = 1


Query: 43  NLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDL 102
           ++E+IK  F++FD N DGKIS +E K+    L  S   +  E +   K  D+DG+GF+DL
Sbjct: 12  SMEDIKKVFQRFDKNNDGKISIDELKDVIGAL--SPNASQEETKAMMKEFDLDGNGFIDL 71

Query: 103 KEFVELYTMS---SGEVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQ 162
            EFV L+ +S   S    + D++ AF +YD + +G+ISA E+  +MK LGE  +++ C++
Sbjct: 72  DEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQR 131

Query: 163 MVKGVDMDGDGFIDVQEFSKLM 182
           M+  VD DGDG +D +EF K+M
Sbjct: 132 MINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of CSPI02G14340 vs. TAIR10
Match: AT5G17470.1 (AT5G17470.1 EF hand calcium-binding protein family)

HSP 1 Score: 109.4 bits (272), Expect = 2.5e-24
Identity = 63/143 (44.06%), Postives = 86/143 (60.14%), Query Frame = 1


Query: 51  FEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYT 110
           FE+ D NKDGKIS++E+ EA R  A S  IT  E +  F+ +DVDGD  +D+ E+     
Sbjct: 7   FERVDKNKDGKISWDEFAEAIR--AFSPSITSEEIDNMFREIDVDGDNQIDVAEYASCL- 66

Query: 111 MSSGEVKVGD----IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDM 170
           M  GE    D    ++ AF +YD +GDGKISA E+  ++K LGE  T+  C  MV+ VD 
Sbjct: 67  MLGGEGNKEDEDIVMKEAFDLYDIDGDGKISASEIHVVLKRLGEKQTIAECIAMVRAVDA 126

Query: 171 DGDGFIDVQEFSKLMGKYSSKFQ 190
           DGDGF+  +EF  +M   + K Q
Sbjct: 127 DGDGFVSFEEFKTMMSCNNKKLQ 146

[Close]

BLAST of CSPI02G14340 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 103.2 bits (256), Expect = 1.8e-22
Identity = 56/142 (39.44%), Postives = 88/142 (61.97%), Query Frame = 1


Query: 41  RPNLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFV 100
           +  + E++  F+KFD N DGKIS +E       L    E+ + E EK+   +D  GDG++
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLG--HEVPEEELEKAITEIDRKGDGYI 91

Query: 101 DLKEFVELYTMSSGEVKV-GDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQ 160
           + +EFVEL T    +  V  +++ AF VYD +G+G ISAEE+  +++ LG+  ++  C++
Sbjct: 92  NFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRK 151

Query: 161 MVKGVDMDGDGFIDVQEFSKLM 182
           M+ GVD DGDG ID +EF  +M
Sbjct: 152 MIGGVDKDGDGTIDFEEFKIMM 171

[Close]

BLAST of CSPI02G14340 vs. NCBI nr
Match: gi|449460606|ref|XP_004148036.1| (PREDICTED: probable calcium-binding protein CML32 [Cucumis sativus])

HSP 1 Score: 366.3 bits (939), Expect = 3.2e-98
Identity = 189/190 (99.47%), Postives = 189/190 (99.47%), Query Frame = 1


Query: 1   MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKQFRPNLEEIKWAFEKFDSNKDG 60
           MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSK FRPNLEEIKWAFEKFDSNKDG
Sbjct: 1   MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDG 60

Query: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 120
           KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD
Sbjct: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 120

Query: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 180
           IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL
Sbjct: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 180

Query: 181 MGKYSSKFQN 191
           MGKYSSKFQN
Sbjct: 181 MGKYSSKFQN 190

[Close]

BLAST of CSPI02G14340 vs. NCBI nr
Match: gi|659115973|ref|XP_008457835.1| (PREDICTED: probable calcium-binding protein CML32 [Cucumis melo])

HSP 1 Score: 347.4 bits (890), Expect = 1.6e-92
Identity = 180/189 (95.24%), Postives = 182/189 (96.30%), Query Frame = 1


Query: 1   MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKQFRPNLEEIKWAFEKFDSNKDG 60
           M+SLLKLFRSCSGKRPSKNTSFSI IKERSNSSS SSK FRPNLEEIKWAFEKFDSNKDG
Sbjct: 1   MTSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDG 60

Query: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 120
           KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVD DGDGFVDLKEFVELYTMSSGEVKVGD
Sbjct: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGD 120

Query: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 180
           IESAFKVYDSNGDGKISAEEVMGIMKILGENTTL ACKQMVKGVDMDGDGFID+QEFSKL
Sbjct: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKL 180

Query: 181 MGKYSSKFQ 190
           M  YSSKFQ
Sbjct: 181 MDTYSSKFQ 189

[Close]

BLAST of CSPI02G14340 vs. NCBI nr
Match: gi|502160872|ref|XP_004511937.1| (PREDICTED: calmodulin-like protein 1 [Cicer arietinum])

HSP 1 Score: 176.4 bits (446), Expect = 4.7e-41
Identity = 100/181 (55.25%), Postives = 129/181 (71.27%), Query Frame = 1


Query: 3   SLLKLFRSCSGKR--PSKNTSFSIPIKERSNSSSPSSKQFRPNLEEIKWAFEKFDSNKDG 62
           S +K   S SGK   P+KN S     K +  +SS  S  F+P  EEIKW FEKFD+NKDG
Sbjct: 5   SFMKFHGSFSGKATSPTKNVSQKNSFKSKQTTSSEMS--FQPQKEEIKWVFEKFDTNKDG 64

Query: 63  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 122
           KIS EEYK A + L   K I DAEA K+FK +D D DGF+D KEF+E++    G++K  +
Sbjct: 65  KISLEEYKAAAKAL--DKGIGDAEAMKAFKAMDSDKDGFIDFKEFMEMFN-GEGKIKEDE 124

Query: 123 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 182
           I+SAF+V+D NGDGKISAEE+  I+K LGE+ +L ACK+MVKGVD DGDGFI++ EF+++
Sbjct: 125 IKSAFQVFDINGDGKISAEELSQILKKLGESCSLSACKKMVKGVDGDGDGFINLNEFTRM 180

[Close]

BLAST of CSPI02G14340 vs. NCBI nr
Match: gi|703106274|ref|XP_010098458.1| (Calmodulin-like protein 1 [Morus notabilis])

HSP 1 Score: 176.0 bits (445), Expect = 6.2e-41
Identity = 85/147 (57.82%), Postives = 113/147 (76.87%), Query Frame = 1


Query: 44  LEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLK 103
           +EE++WAFEKFD+N+DGKIS EEYK A R L   +EITD E  K+F  +D DGDGFVD K
Sbjct: 1   MEELRWAFEKFDTNRDGKISREEYKSALRVL--DREITDGEIAKAFNALDTDGDGFVDFK 60

Query: 104 EFVELYTMSSGEVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKG 163
           EFVE++ M SG VK  DIESAF+++D +GDGKISAEE+  ++K LGE+ +++AC+ MVKG
Sbjct: 61  EFVEMFDMGSGGVKASDIESAFRLFDLDGDGKISAEELSQVLKQLGESCSIRACRNMVKG 120

Query: 164 VDMDGDGFIDVQEFSKLMGKYSSKFQN 191
           VD DGDGFI+++EF+++M        N
Sbjct: 121 VDNDGDGFINIEEFTRMMSTTKKPITN 145

[Close]

BLAST of CSPI02G14340 vs. NCBI nr
Match: gi|470103220|ref|XP_004288041.1| (PREDICTED: calmodulin-like protein 1 [Fragaria vesca subsp. vesca])

HSP 1 Score: 171.4 bits (433), Expect = 1.5e-39
Identity = 95/183 (51.91%), Postives = 124/183 (67.76%), Query Frame = 1


Query: 8   FRSCSGKRPSKNT---SFSIPIKERSNSSSPSSKQFRPNLEEIKWAFEKFDSNKDGKISF 67
           F   S  R S+N      S   KER  SS  S    +P +EEI+W F+KFD+NKDGKIS 
Sbjct: 9   FHYGSSMRKSRNKPAPQLSNVSKERQRSSVSSRSSSQPKMEEIRWVFDKFDTNKDGKISR 68

Query: 68  EEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGDIESA 127
           EEYK   + L   KE+T+AE  K+FK +D DGD  +   EFVE++ M + EVK  DIE A
Sbjct: 69  EEYKSVLKTL--DKEMTEAEIAKTFKALDSDGDDSIGWTEFVEMFNMGADEVKKADIEGA 128

Query: 128 FKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKLMGKY 187
           F+V+D +G+GKISAEE+  I+K LGEN +L ACK+MVKGVD+DGDG ID+ EF+K+MG +
Sbjct: 129 FRVFDLDGNGKISAEELSLILKKLGENCSLGACKKMVKGVDIDGDGLIDLNEFTKMMGSF 188

The following BLAST results are available for this feature:

BLAST of CSPI02G14340 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CML1_ARATH	1.1e-29	43.50	Calmodulin-like protein 1 OS=Arabidopsis thaliana GN=CML1 PE=2 SV=1	[more]
CML24_ARATH	8.1e-25	42.36	Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2	[more]
CML23_ARATH	3.1e-24	41.55	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=...	[more]
CML32_ARATH	4.4e-23	44.06	Probable calcium-binding protein CML32 OS=Arabidopsis thaliana GN=CML32 PE=2 SV=...	[more]
CML25_ARATH	3.2e-21	39.44	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=...	[more]

BLAST of CSPI02G14340 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LMP3_CUCSA	2.2e-98	99.47	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G286450 PE=4 SV=1	[more]
W9RW36_9ROSA	4.3e-41	57.82	Calmodulin-like protein 1 OS=Morus notabilis GN=L484_002706 PE=4 SV=1	[more]
I1LHF1_SOYBN	4.5e-38	50.58	Uncharacterized protein OS=Glycine max GN=GLYMA_11G056500 PE=4 SV=1	[more]
A0A0B2SA84_GLYSO	4.5e-38	50.58	Calmodulin-like protein 1 OS=Glycine soja GN=glysoja_003815 PE=4 SV=1	[more]
G7K7Z2_MEDTR	4.5e-38	51.93	EF hand calcium-binding family protein OS=Medicago truncatula GN=MTR_5g017550 PE...	[more]

BLAST of CSPI02G14340 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G15680.1	6.2e-31	43.50	Calcium-binding EF-hand family protein	[more]
AT5G37770.1	4.6e-26	42.36	EF hand calcium-binding protein family	[more]
AT1G66400.1	1.7e-25	41.55	calmodulin like 23	[more]
AT5G17470.1	2.5e-24	44.06	EF hand calcium-binding protein family	[more]
AT1G24620.1	1.8e-22	39.44	EF hand calcium-binding protein family	[more]

BLAST of CSPI02G14340 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449460606\|ref\|XP_004148036.1\|	3.2e-98	99.47	PREDICTED: probable calcium-binding protein CML32 [Cucumis sativus]	[more]
gi\|659115973\|ref\|XP_008457835.1\|	1.6e-92	95.24	PREDICTED: probable calcium-binding protein CML32 [Cucumis melo]	[more]
gi\|502160872\|ref\|XP_004511937.1\|	4.7e-41	55.25	PREDICTED: calmodulin-like protein 1 [Cicer arietinum]	[more]
gi\|703106274\|ref\|XP_010098458.1\|	6.2e-41	57.82	Calmodulin-like protein 1 [Morus notabilis]	[more]
gi\|470103220\|ref\|XP_004288041.1\|	1.5e-39	51.91	PREDICTED: calmodulin-like protein 1 [Fragaria vesca subsp. vesca]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G14340.1	CSPI02G14340.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 121..181 score: 8.4E-10coord: 45..109 score: 1.6
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 156..184 score: 0.17coord: 46..74 score: 1.7E-6coord: 84..112 score: 1.1E-4coord: 120..148 score: 1.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 152..187 score: 12.114coord: 42..77 score: 15.713coord: 80..115 score: 13.872coord: 116..151 score: 15
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 133..184 score: 1.2E-18coord: 44..132 score: 9.5
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 44..183 score: 1.53
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 93..105 score: -coord: 55..67 score: -coord: 129..141 score: -coord: 165..177 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 39..181 score: 4.0
None	No IPR available	PANTHER	PTHR10891:SF533	SUBFAMILY NOT NAMED	coord: 39..181 score: 4.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI02G14340	Cla020184	Watermelon (97103) v1	cpiwmB142
CSPI02G14340	Csa2G286450	Cucumber (Chinese Long) v2	cpicuB065
CSPI02G14340	MELO3C020900	Melon (DHL92) v3.5.1	cpimeB098
CSPI02G14340	ClCG02G012820	Watermelon (Charleston Gray)	cpiwcgB125
CSPI02G14340	Lsi10G005700	Bottle gourd (USVL1VR-Ls)	cpilsiB091
CSPI02G14340	MELO3C020900.2	Melon (DHL92) v3.6.1	cpimedB083
CSPI02G14340	CsaV3_2G016890	Cucumber (Chinese Long) v3	cpicucB080
CSPI02G14340	Cla97C02G038500	Watermelon (97103) v2	cpiwmbB117
CSPI02G14340	Bhi10G001770	Wax gourd	cpiwgoB172
CSPI02G14340	Cucsa.366800	Cucumber (Gy14) v1	cgycpiB542
CSPI02G14340	CmaCh02G005540	Cucurbita maxima (Rimu)	cmacpiB597
CSPI02G14340	CmoCh02G005640	Cucurbita moschata (Rifu)	cmocpiB576
CSPI02G14340	Cp4.1LG05g11280	Cucurbita pepo (Zucchini)	cpecpiB710
CSPI02G14340	CsGy2G014230	Cucumber (Gy14) v2	cgybcpiB056

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI02G14340	Silver-seed gourd	carcpiB0218
CSPI02G14340	Silver-seed gourd	carcpiB1093
CSPI02G14340	Silver-seed gourd	carcpiB1096
CSPI02G14340	Wax gourd	cpiwgoB180
CSPI02G14340	Wax gourd	cpiwgoB185
CSPI02G14340	Cucurbita maxima (Rimu)	cmacpiB548
CSPI02G14340	Cucurbita maxima (Rimu)	cmacpiB587
CSPI02G14340	Cucurbita maxima (Rimu)	cmacpiB869
CSPI02G14340	Cucurbita moschata (Rifu)	cmocpiB537
CSPI02G14340	Cucurbita moschata (Rifu)	cmocpiB586
CSPI02G14340	Cucurbita moschata (Rifu)	cmocpiB849
CSPI02G14340	Melon (DHL92) v3.5.1	cpimeB092
CSPI02G14340	Cucurbita pepo (Zucchini)	cpecpiB270
CSPI02G14340	Cucurbita pepo (Zucchini)	cpecpiB480
CSPI02G14340	Cucurbita pepo (Zucchini)	cpecpiB712