CSPI02G09060 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI02G09060
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionCytochrome b5
LocationChr2 : 8760741 .. 8763966 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGGACTTCCAAATGAAACACATTGTCTAACACTGATGGCCAAACCAAGTGATCAAGTGTCGGCTGTTTCCTGTTTGAACACAAACCAAGACATCACTGAGGACCAATAACTCACACCCACCACCGGAATTCAAATTCAATTCAATTCAATGCTGCTTCCCTGTCTCCCACTTCGACGCATTTACTCGCTACCCTTTTTATTTCTGCACCGCCCTCACTAGTTTCTGCACTCTTTTTTGGTTCTCTCATTTCTCCGCCGTCTCCGGGCAGATTGGCAAATGGGTGGTGATGGAAAGCTCTTTACATTGGCTGAAGTCTCTCAGCACAACAATCCCAAGGACTGTTGGTTGATCATCAATGGCAAGGTTTTTCTTCATACCCTTTTCTTTGTTTTTGTTTTTGGGATTTCTTCTTTCTATCATGCCTGCAGGAGTTTGATTCTCTGAAGTTGTTTGTTCTTCTTTGCTCTTGAAATCTCACATGCATTCTGGACAGACCCATTATTTTTCACTGTTGAATCAATATGTTTATCCATGGCTGCAAGCTACTGCATGGTGAGCTTTGCCGATGATGTTGTATTGGGCTGGCTAATGAAGGATTTTAGTTGCAGAGTACAGTGTAAATTGAACTATTCCTTTGCCTTTTTTCTTGGGAATGCTAAGAGCTTCAGGCTTCATTTTGTAGCTTTTAAATCATGGCAGCAAACGGTGCTTGAATACGAGGGAAATTAGTGTTTGGTGTATTTAAAACTTAAACGTGAGAAGTAGGATAAAATTTGAGTTTCTGTTGCTGGTTAGAAGGCATCATATCCTTTGGCATTATAATCAGGTTCACCAAACATTAATTACAGTTAGCATTCCCTCTAGTCAGCCACTTTTATCGTTGGCAAAATCAATTTCAAGCGCAGCCTCAATCGTTGAAGAGTGGTGTTTCATCCTCCCCGGGGCTTAGAGGATTGATTTTTGGTTGAGTTAATAAGTTTTGGAAAGGAATGGGTTCTATAAATGTGTACTCATATCACGTATATTTGGTTTAGGAGTTTTTGATCTATAAAGTATTATAAAGTGGATTTTATAAGCCTGAGAATGTGTAACATTGGTTAATGTAGTTCATGAGGTTAACAAACAAATTTGCATACGAAGATAAACAAACTCACATGATGGCAAGCATAGATTTGGCTTCTTAATTTCGGAATATACATTTCTTGCTCTGCTGTGCTCACCTGACCAAGTATATAAGACTGAACTGGAGATTTATGATTTATAAGATCTTAAAAAATGCGCTGTGTTTAGTGTCTTGAAGGGACTTATTTTGTGTCTTTGATTCCTTTAGGACCAGGATCTCATTGTATGGATAGAATCTAAGCCTTATTCATGTTTGGCAGTCCTGTTATGAAACAAATGCTTTCATATTTTTGCCAATTCACTGACAGGAATATCAGTGTAGAAAAACTTTGTTTCAAGAGCGGTTAAATATGAAGATCACTGACTTGAAAATGAACTGATCCAAAGATCTCATTTTATTTTTGTGTGTGTGAATGCTCTTATCTACCGAGGGAGGTCTTATGCATATACTATGTGAGAACTGGACTCCAGTGGGGGTTGGACGTCCAACACTTCGTTCAAACCTAACCAATAAAAAAATCAGACGTCTGGGGGATGAATAGTCAACATACATCGTTGTTATGCTGTCTGGTGGGTCAATGTCTGTCACTGCAGACCACATCTACCATAAAAAAATTCTTTCATTCTGATTCTTCTGAATCCAATCGATGATTTTTTTCTTTGGCATCCATAATATGATCTCAAAGCTATATCCTGAAGCCTCAGTCTGTTGAATTTGCACATTCTATTGATGTTAAAAATTTCCTGCCAGCCATCAGACTTACGTCCGGCCCTACTAATCTTCAAGATGGCTTCATCCACACATTGGAAAGATTCATGACATACAAAGAGTTTTAAAATATTGACTTCCCCTACCTCGGCTTTAAAGTTGAACCCAGATATTTCAAGTAGTTCTCTAGAATTAAAGTCCTTTACACTAAAGCCATCCTTGGGGTAAAATCAGAAATTTAGTGTCCAATTAGTAGTGGGAAGCTCTTGCTGGCTGTACTTGTTCATCACTCGTTCTTATCAATGTAATGTGAAATCTCTTTCTTGACCTACCATATCAATAAAGGATAAAGATACTCTAGGAATGCTAATTGTGTTGGAGAATGTCAAGTACTAGTTTCTCAACAACCAAAACTAGATAACCAAGACCATTAGCCAACTCAAGTTTCTTAAGGTTCTAAGTGTAAAGTTAGAAGCAGGAGGATAACATGAAAATGCATGAAACTTCCCTCAGCTTTGCCCAATTCCTACTCGGAAGTGAATATAAATTATATCTTCTCAAAGAACAACACTTGTGCATTTCTACTATTGTTTTTCTTCTAAAATTTCTTGAATTATAGTAATAGAAAGTCGAATAATTTTGATTTTCTCACTTGTACTATGAGTTCAGAATGGGATCAACAAGCTTTCAGTTGTTTTGGCAGTTGGCCTGGAGTTAAACTATATGAATCATGCACAGATTTTATCTTTGATGGGTATTGGTTTTTAGCTTGAGGTTACCATTTTGTCCATCTTTGATTGTCTCAACAGGTTTACAATGTAACCGAGTTCTTGGAAGATCATCCCGGTGGTGACGAGGTGTTGTTGTCTGGCACAGGTAATAAGTCCTCCAGTCATACAGCATTAGTTATTTACAATGTACATTGAGTGTTTGTGCATCGTTTACACACTGTAATTTTTATGACCACAGGTAAGGATGCAACTGATGATTTTGAAGACGTGGGTCATAGTGAGAGCGCAAGAGAAACGATGAGCAAATACTACGTTGGAGAGATCGACATCTCGACACTCCCAGAACGGTTGACATACACAGGTCCAAAGCAGCCTCATTACAATCAGGATAAGACAACCGAGTTCATTATTAAGCTCCTCCAGTTCTTGGTTCCTCTCGCAATCTTGGGATTGGCGGTTGGTATCCGCTTTTACAACAAATCAAGCTGAGGTTCTGGTTGAGATATTATGTGATGTAGTCTTATGATCAATTACAAACCGCTGCATTGTGTTTGTGAAACTGAGAAATTTTCTACTCTAATGCTCTACTTTTTTTTTCTCTTCCTTTTCTGTGTCTTTTACATTTTAGTTCTGCTATCTGATTGGTAAAGTAAATATAATTT

mRNA sequence

ATGGGTGGTGATGGAAAGCTCTTTACATTGGCTGAAGTCTCTCAGCACAACAATCCCAAGGACTGTTGGTTGATCATCAATGGCAAGGTTTACAATGTAACCGAGTTCTTGGAAGATCATCCCGGTGGTGACGAGGTGTTGTTGTCTGGCACAGGTAAGGATGCAACTGATGATTTTGAAGACGTGGGTCATAGTGAGAGCGCAAGAGAAACGATGAGCAAATACTACGTTGGAGAGATCGACATCTCGACACTCCCAGAACGGTTGACATACACAGGTCCAAAGCAGCCTCATTACAATCAGGATAAGACAACCGAGTTCATTATTAAGCTCCTCCAGTTCTTGGTTCCTCTCGCAATCTTGGGATTGGCGGTTGGTATCCGCTTTTACAACAAATCAAGCTGA

Coding sequence (CDS)

ATGGGTGGTGATGGAAAGCTCTTTACATTGGCTGAAGTCTCTCAGCACAACAATCCCAAGGACTGTTGGTTGATCATCAATGGCAAGGTTTACAATGTAACCGAGTTCTTGGAAGATCATCCCGGTGGTGACGAGGTGTTGTTGTCTGGCACAGGTAAGGATGCAACTGATGATTTTGAAGACGTGGGTCATAGTGAGAGCGCAAGAGAAACGATGAGCAAATACTACGTTGGAGAGATCGACATCTCGACACTCCCAGAACGGTTGACATACACAGGTCCAAAGCAGCCTCATTACAATCAGGATAAGACAACCGAGTTCATTATTAAGCTCCTCCAGTTCTTGGTTCCTCTCGCAATCTTGGGATTGGCGGTTGGTATCCGCTTTTACAACAAATCAAGCTGA
Homology
[Close]
BLAST of CSPI02G09060 vs. Swiss-Prot
Match: CYB5B_ARATH (Cytochrome b5 isoform B OS=Arabidopsis thaliana GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 9.4e-60
Identity = 105/133 (78.95%), Postives = 120/133 (90.23%), Query Frame = 1 

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MG + K+FTL+EVS+HN   DCW++INGKVYNVT+FLEDHPGGD+VLLS TGKDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESARE M +YYVGEID +T+P+++ YT PKQPHYNQDKT+EFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKS 134
           LGLAVGIR Y KS
Sbjct: 121 LGLAVGIRIYTKS 133

[Close]
BLAST of CSPI02G09060 vs. Swiss-Prot
Match: CYB5S_TOBAC (Cytochrome b5, seed isoform OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 3.9e-58
Identity = 102/134 (76.12%), Postives = 115/134 (85.82%), Query Frame = 1 

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGG  K+FTLAEVS HNN KDCWLII+GKVYNVT+FLEDHPGG EVLLS TGKDATDDFE
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           D+GHS SAR  + +YYVG+ID ST+P ++ YT PKQPHYNQDKTTEFI+KLLQFLVPL I
Sbjct: 61  DIGHSSSARAMLDEYYVGDIDSSTIPTKVKYTPPKQPHYNQDKTTEFIVKLLQFLVPLII 120

Query: 121 LGLAVGIRFYNKSS 135
           LG+A G+ FY K S
Sbjct: 121 LGVAFGVHFYTKQS 134

[Close]
BLAST of CSPI02G09060 vs. Swiss-Prot
Match: CYB5_TOBAC (Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 1.5e-57
Identity = 100/134 (74.63%), Postives = 117/134 (87.31%), Query Frame = 1 

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGG+ K+FTLAEVSQHNN KDCWL+I+GKVY+VT+FL+DHPGGDEVLLS TGKDATDDFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHS SAR  + +YYVG+ID +T+P +  YT P QPHYNQDKT+EF++KLLQFLVPL I
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLII 120

Query: 121 LGLAVGIRFYNKSS 135
           LG+A GIRFY K S
Sbjct: 121 LGVAFGIRFYTKQS 134

[Close]
BLAST of CSPI02G09060 vs. Swiss-Prot
Match: CYB5_BOROF (Cytochrome b5 OS=Borago officinalis PE=2 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 1.2e-54
Identity = 97/130 (74.62%), Postives = 113/130 (86.92%), Query Frame = 1 

Query: 5   GKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGH 64
           GK+FTLAEV+QHNN KDCWLIINGKVY+VT+FLEDHPGGD+VLLS TGKDATDDFED+GH
Sbjct: 2   GKIFTLAEVAQHNNSKDCWLIINGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFEDIGH 61

Query: 65  SESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAILGLA 124
           S SA+  + +YYVG+ID S++P ++ YT PKQP YN DKT EF+IKLLQFLVPL IL  A
Sbjct: 62  SSSAKAMLDEYYVGDIDSSSIPSQVKYTPPKQPLYNPDKTREFVIKLLQFLVPLVILAGA 121

Query: 125 VGIRFYNKSS 135
           +GIRFY KSS
Sbjct: 122 IGIRFYTKSS 131

[Close]
BLAST of CSPI02G09060 vs. Swiss-Prot
Match: CYB5_ORYSJ (Cytochrome b5 OS=Oryza sativa subsp. japonica GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 208.8 bits (530), Expect = 3.8e-53
Identity = 96/128 (75.00%), Postives = 109/128 (85.16%), Query Frame = 1 

Query: 6   KLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHS 65
           K++TL EV++HN+  DCWLII GKVYNV++FLEDHPGGD+VLLS TGKDATDDFEDVGHS
Sbjct: 7   KVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGHS 66

Query: 66  ESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAILGLAV 125
            +AR  M +YYVG+ID ST+P R  Y  PKQPHYNQDKT EFIIK+LQFLVPLAILGLAV
Sbjct: 67  TTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPEFIIKILQFLVPLAILGLAV 126

Query: 126 GIRFYNKS 134
            IR Y KS
Sbjct: 127 AIRIYTKS 134

[Close]
BLAST of CSPI02G09060 vs. TrEMBL
Match: A0A0A0LHS9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G138200 PE=3 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 1.5e-72
Identity = 133/134 (99.25%), Postives = 133/134 (99.25%), Query Frame = 1 

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKLFT AEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLFTFAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKSS 135
           LGLAVGIRFYNKSS
Sbjct: 121 LGLAVGIRFYNKSS 134

[Close]
BLAST of CSPI02G09060 vs. TrEMBL
Match: V4RJB6_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10006192mg PE=3 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 1.3e-60
Identity = 112/133 (84.21%), Postives = 122/133 (91.73%), Query Frame = 1 

Query: 2   GGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFED 61
           GGDGK+FTLAEVS HNN KDCWLIINGKVY+VT+FLEDHPGGDEVLLS TGKDATDDFED
Sbjct: 3   GGDGKVFTLAEVSDHNNMKDCWLIINGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFED 62

Query: 62  VGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAIL 121
           VGHS SARE M +YYVGEID+ST+P++  YT PKQPHYNQDKT+EFII+LLQFLVPLAIL
Sbjct: 63  VGHSPSAREMMDQYYVGEIDVSTIPKKKEYTPPKQPHYNQDKTSEFIIRLLQFLVPLAIL 122

Query: 122 GLAVGIRFYNKSS 135
           GLAVGIR Y KSS
Sbjct: 123 GLAVGIRIYTKSS 135

[Close]
BLAST of CSPI02G09060 vs. TrEMBL
Match: A0A061EKX2_THECC (Cytochrome B5, n4,ATCB5-B,CB5-B OS=Theobroma cacao GN=TCM_020340 PE=3 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 1.3e-60
Identity = 110/132 (83.33%), Postives = 122/132 (92.42%), Query Frame = 1 

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKLFTLA+VS+HN PKDCWLIINGKVY+VT+FLEDHPGGDEVLLS TGKDATDDFE
Sbjct: 1   MGGDGKLFTLAQVSEHNTPKDCWLIINGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHS+SAR+ M +YYVGEID+ST+P++  Y  PKQPHYNQDKT+EFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDSARDMMDQYYVGEIDVSTIPKKTKYKPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNK 133
           LGLAVGIR Y K
Sbjct: 121 LGLAVGIRIYTK 132

[Close]
BLAST of CSPI02G09060 vs. TrEMBL
Match: A0A061DMJ6_THECC (Cytochrome B5, n4,ATCB5-B,CB5-B OS=Theobroma cacao GN=TCM_002942 PE=3 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 5.5e-59
Identity = 107/133 (80.45%), Postives = 120/133 (90.23%), Query Frame = 1 

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGG GK++TLAEVSQHNNPKDCWL+I GKVYNVT+FLEDHPGGDEVLLS TGKDATDDFE
Sbjct: 1   MGGRGKVYTLAEVSQHNNPKDCWLVIEGKVYNVTKFLEDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHS SAR  M ++YVG+ID ST+P ++ YT PKQPHY+QDKT+EF+IKLLQFLVPL I
Sbjct: 61  DVGHSSSARAMMDEFYVGDIDTSTIPTKIKYTPPKQPHYDQDKTSEFVIKLLQFLVPLLI 120

Query: 121 LGLAVGIRFYNKS 134
           LGLAVGIRFY KS
Sbjct: 121 LGLAVGIRFYTKS 133

[Close]
BLAST of CSPI02G09060 vs. TrEMBL
Match: Q5V9L2_VERFO (Cytochrome b5 isoform Cb5-C OS=Vernicia fordii PE=2 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 1.2e-58
Identity = 107/134 (79.85%), Postives = 118/134 (88.06%), Query Frame = 1 

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MG  GK+FTLAEVS+HNNPKDCWL+I GKVY+VT+FLEDHPGGDEVLLS TGKDATDDFE
Sbjct: 1   MGSSGKVFTLAEVSEHNNPKDCWLVIEGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHS SAR  M ++YVGEID ST+P R+ YT PKQPHYNQDKT EFIIK LQF+VPL I
Sbjct: 61  DVGHSSSARAMMDEFYVGEIDSSTIPSRMAYTPPKQPHYNQDKTMEFIIKFLQFVVPLLI 120

Query: 121 LGLAVGIRFYNKSS 135
           LGLAVGIRFY KS+
Sbjct: 121 LGLAVGIRFYTKSA 134

[Close]
BLAST of CSPI02G09060 vs. TAIR10
Match: AT2G32720.1 (AT2G32720.1 cytochrome B5 isoform B)

HSP 1 Score: 230.7 bits (587), Expect = 5.3e-61
Identity = 105/133 (78.95%), Postives = 120/133 (90.23%), Query Frame = 1 

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MG + K+FTL+EVS+HN   DCW++INGKVYNVT+FLEDHPGGD+VLLS TGKDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESARE M +YYVGEID +T+P+++ YT PKQPHYNQDKT+EFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKS 134
           LGLAVGIR Y KS
Sbjct: 121 LGLAVGIRIYTKS 133

[Close]
BLAST of CSPI02G09060 vs. TAIR10
Match: AT5G53560.1 (AT5G53560.1 cytochrome B5 isoform E)

HSP 1 Score: 196.1 bits (497), Expect = 1.4e-50
Identity = 88/132 (66.67%), Postives = 108/132 (81.82%), Query Frame = 1 

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           M  D K+ +  EVS+HN  KDCWLII+GKVY+VT F++DHPGGDEVLLS TGKDAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHS++AR+ M KY++GEID S++P   TY  P+QP YNQDKT EFIIK+LQFLVP+ I
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 121 LGLAVGIRFYNK 133
           LGLA+ +R Y K
Sbjct: 121 LGLALVVRHYTK 132

[Close]
BLAST of CSPI02G09060 vs. TAIR10
Match: AT5G48810.1 (AT5G48810.1 cytochrome B5 isoform D)

HSP 1 Score: 190.7 bits (483), Expect = 6.0e-49
Identity = 85/135 (62.96%), Postives = 112/135 (82.96%), Query Frame = 1 

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGK+FTL+EVSQH++ KDCW++I+GKVY+VT+FL+DHPGGDEV+L+ TGKDATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPH--YNQDKTTEFIIKLLQFLVPL 120
           DVGHS +A+  + +YYVG+ID +T+P +  +  P        QDK+++F+IKLLQFLVPL
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 AILGLAVGIRFYNKS 134
            ILGLA GIR+Y K+
Sbjct: 121 LILGLAFGIRYYTKT 135

[Close]
BLAST of CSPI02G09060 vs. TAIR10
Match: AT2G46650.1 (AT2G46650.1 cytochrome B5 isoform C)

HSP 1 Score: 131.0 bits (328), Expect = 5.7e-31
Identity = 61/127 (48.03%), Postives = 88/127 (69.29%), Query Frame = 1 

Query: 7   LFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHSE 66
           L +  +V++H    DCW++I+GKVY+++ F+++HPGGD VLL+ TGKDA+ DFEDV HS+
Sbjct: 4   LISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSK 63

Query: 67  SARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTT--EFIIKLLQFLVPLAILGLA 126
            A+E M KY +G++D ST+P    Y  P +      +TT  E   KLL +L+PL ILG+A
Sbjct: 64  DAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGVA 123

Query: 127 VGIRFYN 132
             +RFYN
Sbjct: 124 FALRFYN 130

[Close]
BLAST of CSPI02G09060 vs. TAIR10
Match: AT1G26340.1 (AT1G26340.1 cytochrome B5 isoform A)

HSP 1 Score: 127.9 bits (320), Expect = 4.8e-30
Identity = 57/127 (44.88%), Postives = 90/127 (70.87%), Query Frame = 1 

Query: 6   KLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHS 65
           KL+++ E + HN   DCW++I+GKVY+V+ ++++HPGGD+VLL+  GKDATDDFED GHS
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66  ESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQ-----FLVPLAI 125
           + ARE M KY++GE+D S+LPE      P+   Y +D+  + + KL       ++VP++I
Sbjct: 66  KDARELMEKYFIGELDESSLPEI-----PELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 125

Query: 126 LGLAVGI 128
           + ++V +
Sbjct: 126 ITISVAV 127

[Close]
BLAST of CSPI02G09060 vs. NCBI nr
Match: gi|449442375|ref|XP_004138957.1| (PREDICTED: cytochrome b5 isoform B [Cucumis sativus])

HSP 1 Score: 280.0 bits (715), Expect = 2.1e-72
Identity = 133/134 (99.25%), Postives = 133/134 (99.25%), Query Frame = 1 

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKLFT AEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLFTFAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKSS 135
           LGLAVGIRFYNKSS
Sbjct: 121 LGLAVGIRFYNKSS 134

[Close]
BLAST of CSPI02G09060 vs. NCBI nr
Match: gi|659114830|ref|XP_008457249.1| (PREDICTED: cytochrome b5 isoform B-like [Cucumis melo])

HSP 1 Score: 275.4 bits (703), Expect = 5.3e-71
Identity = 129/134 (96.27%), Postives = 133/134 (99.25%), Query Frame = 1 

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKL+TLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHSESARE MSKYY+GEIDIST+PE+LTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNKSS 135
           LGLAVGIRFYNKSS
Sbjct: 121 LGLAVGIRFYNKSS 134

[Close]
BLAST of CSPI02G09060 vs. NCBI nr
Match: gi|567855547|ref|XP_006420893.1| (hypothetical protein CICLE_v10006192mg [Citrus clementina])

HSP 1 Score: 240.4 bits (612), Expect = 1.9e-60
Identity = 112/133 (84.21%), Postives = 122/133 (91.73%), Query Frame = 1 

Query: 2   GGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFED 61
           GGDGK+FTLAEVS HNN KDCWLIINGKVY+VT+FLEDHPGGDEVLLS TGKDATDDFED
Sbjct: 3   GGDGKVFTLAEVSDHNNMKDCWLIINGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFED 62

Query: 62  VGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAIL 121
           VGHS SARE M +YYVGEID+ST+P++  YT PKQPHYNQDKT+EFII+LLQFLVPLAIL
Sbjct: 63  VGHSPSAREMMDQYYVGEIDVSTIPKKKEYTPPKQPHYNQDKTSEFIIRLLQFLVPLAIL 122

Query: 122 GLAVGIRFYNKSS 135
           GLAVGIR Y KSS
Sbjct: 123 GLAVGIRIYTKSS 135

[Close]
BLAST of CSPI02G09060 vs. NCBI nr
Match: gi|590656842|ref|XP_007034387.1| (Cytochrome B5, n4,ATCB5-B,CB5-B [Theobroma cacao])

HSP 1 Score: 240.4 bits (612), Expect = 1.9e-60
Identity = 110/132 (83.33%), Postives = 122/132 (92.42%), Query Frame = 1 

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGGDGKLFTLA+VS+HN PKDCWLIINGKVY+VT+FLEDHPGGDEVLLS TGKDATDDFE
Sbjct: 1   MGGDGKLFTLAQVSEHNTPKDCWLIINGKVYDVTKFLEDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHS+SAR+ M +YYVGEID+ST+P++  Y  PKQPHYNQDKT+EFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDSARDMMDQYYVGEIDVSTIPKKTKYKPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVGIRFYNK 133
           LGLAVGIR Y K
Sbjct: 121 LGLAVGIRIYTK 132

[Close]
BLAST of CSPI02G09060 vs. NCBI nr
Match: gi|590713947|ref|XP_007049778.1| (Cytochrome B5, n4,ATCB5-B,CB5-B [Theobroma cacao])

HSP 1 Score: 235.0 bits (598), Expect = 7.9e-59
Identity = 107/133 (80.45%), Postives = 120/133 (90.23%), Query Frame = 1 

Query: 1   MGGDGKLFTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60
           MGG GK++TLAEVSQHNNPKDCWL+I GKVYNVT+FLEDHPGGDEVLLS TGKDATDDFE
Sbjct: 1   MGGRGKVYTLAEVSQHNNPKDCWLVIEGKVYNVTKFLEDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120
           DVGHS SAR  M ++YVG+ID ST+P ++ YT PKQPHY+QDKT+EF+IKLLQFLVPL I
Sbjct: 61  DVGHSSSARAMMDEFYVGDIDTSTIPTKIKYTPPKQPHYDQDKTSEFVIKLLQFLVPLLI 120

Query: 121 LGLAVGIRFYNKS 134
           LGLAVGIRFY KS
Sbjct: 121 LGLAVGIRFYTKS 133

The following BLAST results are available for this feature:
BLAST of CSPI02G09060 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CYB5B_ARATH9.4e-6078.95Cytochrome b5 isoform B OS=Arabidopsis thaliana GN=CYTB5-B PE=1 SV=1[more]
CYB5S_TOBAC3.9e-5876.12Cytochrome b5, seed isoform OS=Nicotiana tabacum PE=2 SV=1[more]
CYB5_TOBAC1.5e-5774.63Cytochrome b5 OS=Nicotiana tabacum PE=2 SV=1[more]
CYB5_BOROF1.2e-5474.62Cytochrome b5 OS=Borago officinalis PE=2 SV=1[more]
CYB5_ORYSJ3.8e-5375.00Cytochrome b5 OS=Oryza sativa subsp. japonica GN=Os05g0108800 PE=2 SV=2[more]
BLAST of CSPI02G09060 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LHS9_CUCSA1.5e-7299.25Uncharacterized protein OS=Cucumis sativus GN=Csa_2G138200 PE=3 SV=1[more]
V4RJB6_9ROSI1.3e-6084.21Uncharacterized protein OS=Citrus clementina GN=CICLE_v10006192mg PE=3 SV=1[more]
A0A061EKX2_THECC1.3e-6083.33Cytochrome B5, n4,ATCB5-B,CB5-B OS=Theobroma cacao GN=TCM_020340 PE=3 SV=1[more]
A0A061DMJ6_THECC5.5e-5980.45Cytochrome B5, n4,ATCB5-B,CB5-B OS=Theobroma cacao GN=TCM_002942 PE=3 SV=1[more]
Q5V9L2_VERFO1.2e-5879.85Cytochrome b5 isoform Cb5-C OS=Vernicia fordii PE=2 SV=1[more]
BLAST of CSPI02G09060 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G32720.15.3e-6178.95 cytochrome B5 isoform B[more]
AT5G53560.11.4e-5066.67 cytochrome B5 isoform E[more]
AT5G48810.16.0e-4962.96 cytochrome B5 isoform D[more]
AT2G46650.15.7e-3148.03 cytochrome B5 isoform C[more]
AT1G26340.14.8e-3044.88 cytochrome B5 isoform A[more]
BLAST of CSPI02G09060 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449442375|ref|XP_004138957.1|2.1e-7299.25PREDICTED: cytochrome b5 isoform B [Cucumis sativus][more]
gi|659114830|ref|XP_008457249.1|5.3e-7196.27PREDICTED: cytochrome b5 isoform B-like [Cucumis melo][more]
gi|567855547|ref|XP_006420893.1|1.9e-6084.21hypothetical protein CICLE_v10006192mg [Citrus clementina][more]
gi|590656842|ref|XP_007034387.1|1.9e-6083.33Cytochrome B5, n4,ATCB5-B,CB5-B [Theobroma cacao][more]
gi|590713947|ref|XP_007049778.1|7.9e-5980.45Cytochrome B5, n4,ATCB5-B,CB5-B [Theobroma cacao][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001199Cyt_B5-like_heme/steroid-bd
IPR018506Cyt_B5_heme-BS
Vocabulary: Molecular Function
TermDefinition
GO:0020037heme binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0043447 alkane biosynthetic process
biological_process GO:0019745 pentacyclic triterpenoid biosynthetic process
biological_process GO:0016126 sterol biosynthetic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0046872 metal ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G09060.1CSPI02G09060.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001199Cytochrome b5-like heme/steroid binding domainPRINTSPR00363CYTOCHROMEB5coord: 40..54
score: 7.8E-15coord: 30..40
score: 7.8E-15coord: 68..80
score: 7.8E-15coord: 55..62
score: 7.8
IPR001199Cytochrome b5-like heme/steroid binding domainGENE3DG3DSA:3.10.120.10coord: 6..86
score: 4.4
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 9..80
score: 3.9
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 8..81
score: 1.6
IPR001199Cytochrome b5-like heme/steroid binding domainPROFILEPS50255CYTOCHROME_B5_2coord: 5..81
score: 29
IPR001199Cytochrome b5-like heme/steroid binding domainunknownSSF55856Cytochrome b5-like heme/steroid binding domaincoord: 6..89
score: 9.29
IPR018506Cytochrome b5, heme-binding sitePROSITEPS00191CYTOCHROME_B5_1coord: 36..43
scor
NoneNo IPR availablePANTHERPTHR19359CYTOCHROME B5coord: 6..134
score: 7.9
NoneNo IPR availablePANTHERPTHR19359:SF26CYTOCHROME B5 ISOFORM Bcoord: 6..134
score: 7.9

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI02G09060Cla002950Watermelon (97103) v1cpiwmB170
CSPI02G09060Cla021423Watermelon (97103) v1cpiwmB134
CSPI02G09060Csa2G138200Cucumber (Chinese Long) v2cpicuB065
CSPI02G09060Csa3G131950Cucumber (Chinese Long) v2cpicuB072
CSPI02G09060MELO3C020457Melon (DHL92) v3.5.1cpimeB134
CSPI02G09060MELO3C006316Melon (DHL92) v3.5.1cpimeB147
CSPI02G09060ClCG05G003370Watermelon (Charleston Gray)cpiwcgB149
CSPI02G09060ClCG06G007700Watermelon (Charleston Gray)cpiwcgB150
CSPI02G09060Lsi09G011520Bottle gourd (USVL1VR-Ls)cpilsiB088
CSPI02G09060Lsi05G018330Bottle gourd (USVL1VR-Ls)cpilsiB124
CSPI02G09060MELO3C020457.2Melon (DHL92) v3.6.1cpimedB127
CSPI02G09060MELO3C006316.2Melon (DHL92) v3.6.1cpimedB141
CSPI02G09060CsaV3_2G011470Cucumber (Chinese Long) v3cpicucB080
CSPI02G09060CsaV3_3G011020Cucumber (Chinese Long) v3cpicucB100
CSPI02G09060Cla97C05G084020Watermelon (97103) v2cpiwmbB142
CSPI02G09060Cla97C06G117430Watermelon (97103) v2cpiwmbB144
CSPI02G09060Bhi12G001309Wax gourdcpiwgoB126
CSPI02G09060Cucsa.256410Cucumber (Gy14) v1cgycpiB377
CSPI02G09060Cucsa.266330Cucumber (Gy14) v1cgycpiB403
CSPI02G09060CmaCh14G018900Cucurbita maxima (Rimu)cmacpiB239
CSPI02G09060CmaCh17G001070Cucurbita maxima (Rimu)cmacpiB355
CSPI02G09060CmoCh14G019410Cucurbita moschata (Rifu)cmocpiB227
CSPI02G09060CmoCh17G001120Cucurbita moschata (Rifu)cmocpiB351
CSPI02G09060Cp4.1LG12g00830Cucurbita pepo (Zucchini)cpecpiB132
CSPI02G09060CsGy3G010980Cucumber (Gy14) v2cgybcpiB109
CSPI02G09060CsGy2G009320Cucumber (Gy14) v2cgybcpiB056
CSPI02G09060Carg10136Silver-seed gourdcarcpiB0258
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CSPI02G09060CSPI03G10920Wild cucumber (PI 183967)cpicpiB071
The following block(s) are covering this gene:
GeneOrganismBlock
CSPI02G09060Silver-seed gourdcarcpiB1111
CSPI02G09060Cucumber (Chinese Long) v3cpicucB093
CSPI02G09060Wild cucumber (PI 183967)cpicpiB063
CSPI02G09060Cucurbita maxima (Rimu)cmacpiB358
CSPI02G09060Cucurbita maxima (Rimu)cmacpiB902
CSPI02G09060Cucurbita moschata (Rifu)cmocpiB882
CSPI02G09060Melon (DHL92) v3.5.1cpimeB145
CSPI02G09060Watermelon (97103) v1cpiwmB128
CSPI02G09060Cucurbita pepo (Zucchini)cpecpiB304
CSPI02G09060Bottle gourd (USVL1VR-Ls)cpilsiB128
CSPI02G09060Melon (DHL92) v3.6.1cpimedB139