CSPI02G07750 (gene) Wild cucumber (PI 183967)

NameCSPI02G07750
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionGH3 family protein
LocationChr2 : 6820728 .. 6825736 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTAACCCGAATCTTAAAAATAATCTACAAAGATGCCGTCACATGTTGAAGAGAGTTGGTTGGATGAGAGAGATAAGGAAGTACTACAACACATTCAGCATATCACAAGCAATGCTGGCGAAATCCAACGACAAATTCTAAGTGAAATTCTGTCAACGAATGCTAATGTTGAATATTTACAACAACATGGCCTTCATGCCTCTACTGGTTCTTCAACCTTCAAAAAACTTATACCTCTTGTCTCTTACGAACAATTAAAACCTTATATAACTCGTATTGCAGAAGGTGATGATTCCCCAATCCTATGCTCAAACCCCATTACAGCGTTCTTTCTAAGGTGACAAACACACACACACACACACACATATATATTTCATATACTCGCCCTATCTGTTTTTTTGTTTTAAATTAATTCTTTTATATGTCTTGTTGTAGCTCTGGGACATCTGGGGGAGAACCCAAGTTAGTTCCAATATATGAAAAAGAGTTTGAGAGAAGGTTGTCTTTTTTCAACTATTTGATGGCAAGGACGAAAGAATTGTTTCCAAATATCAATTGGCACAAAGGTAAAGCTATGAACTTTCACTTTGCAAAGCCTGATCATAAGACAAAAGCTGGAATTCTTGTTCATACTGTATTTTCAAGACTGCTCAAAAGATCACTAAATCTCAAAAGTGTAGAAAGTGGGAATAACGCAATCCCTGATGACATTCTTCGATGCACGAACACATACCAAAGCTTATACTGTCAGCTTCTTTGTGGGCTCTACCAAAACGATCTAGTTTTTCAAGTTGGTGCAGTTTTGGCTTCTGGGTTGATTCACGTTTTTAAGTTCCTTGAAAATCATTGGGTTGATTTGGTTAGTGATATACGTAGAGGAAGTATTAATAACCCCAAAATTACAGACTTATCACTGAGAGAATCAGTTATGAAGATTCTTGTAAAACCCAATCCTCAACTTGCAGATTTGATTGAAACTGAATGTAGCAAAGGAAAATGGAAAGGAATTGTTCCAAAATTGTGGCCAAACACTAAATATATTAAGGCTATTGCCACAGGAAGCTTGTCACAATACATTCCATTGCTAAATTACTACACAAACAACCTCCCTATATTTTCTGACCATTATGGTTCTACTGAATGTTTCCTTGGCTTGAATCTGGATCCAATATGTGACCCTAATGAAACCTCCTACACTTTGATCCCTACCATGGCGTATTTCGAGTTCTTACCAATAGATACCACAAATATCAATGGCGAAGTGACCCAAGAATTAGTTGATCTTGTTGATGTCAAGTTAGGACAAGAATATGAGCTCGTCATCACCACTTTCGCTGGTAAATGACCTCTTTTCCTTTCTCATACTATATTTATTCAATATTCATATCTGCATAAATTTCAAAGTTCAGTCTCATAACTTCAAATTCTGTTGTGTACGTGTTCGCTAACTTATATAGCTAACGTATATTTTTTGATAAACTCGAATCTTTCAAAAAGAAAATTAACTAATATATTATTAAATGTAAAATTGAACTTATATACGCTATGATCGGAGTAGATATTTGAATTGTGGTGTTTGTTGCATATTGTGTAAGATATCACTTTGCATTTAGATATTCTTCACCCTACGATCACGTTCTTATAGGCCGTATATTCGGAGTTTTGAAACTACAGCCCCGAAATCCACTCGATTTGACATTTTTCTTAATGAGAAGAATAACATTTCAAACCAGTATAAACAGTACTAATCTAGAAGAAGATTTAATTTAAATTAATAAATAACGGCATGCAAATATATATATTGTTTAATTAAGTTTATTTAACGGAGTTGAAGGAAACGATAAAACAGCTAAAAAATATTTTATATTAATATAACTATTTTTCCATAACAAATATATGTCTATGAAAAGATTTTAGACTTCTATAATTGTTGTTATTTATAACTTAGGGATCTAAAAATTAGAATTATCGTGGACAAGAGGTCTCAGATTTAAATCCTCACTCCAACATTTATTACAATACCTTTGTAAAAAAAAATTAAAGTTATTTTATAAGTTTTAATTTTGTTTTCAGTACATTTATGGTTTTGGAAATTAGACCTATTTCATTCTCATTTTGTACAGTATTTTTCTTAAGTGTTGATTTCTGAGACAAAGTATGAAAAGAAAAACAAGTTTTTAAAAGCTACTGTTTTTTTGTTTTCTAATTTTTGTTTGAATTTTTCAATGATTGGTAAAAAGTAAATAACATATCAACAAGACATTTGAAGGTGAAATAAGTGTCTATACAGTTAGTTTTTAAAAATAGAAATAAAAAATAAAATGATTACCAAATGAGCCTTACTTTTTTGTTTTTAAAATTAATTAAGCCTATAATAAAACATTAATTCTACATCTAAATTTCTTTATTTTTTACTGTTTACTAAATACCATTTAATTATTTGAAAATCCAAGGAAAATTTTGAAGACTGAAAAAAAGTAGTTTAAAAAATAAACAATTTTTGTAAGGCTCTTGATTGCATACCGCCAACGCGATATTGACCCTGCGCCAAGGATGTTGAGAGAAGAGATTTATAATAGTAGATCAAATATATATATTCCGTGAAAGGAGTATGCATGAGACTCTAACCCGAAGAAAGTAATTCCAAATGGAGAGGATCCTCGTTGAAAAGTTATTTTGGATAGAAGTATAGGATATTCTGCGAAGGAGCGTGAAAAGAGAAGGAGACGACACGAAGAGAAATGCTTGTCTAAGTCATATCGTGTGAGTGTAAGCGATGTAATAAAAGTAAGTAGCAAGGAATAGGTCTACGTTGAATGTTAAAAGTTAAAGGTTTAATAGTGTAGAAAGAACTACCCATGCCTACTAGAGTGCGACAAGAAGTAGGTGCCCTGTTGACGTGACTGGACGATCCCATAAAGTATGGATCTGATGTTTCCCAAGAAGATAAGGGAGAGTCTGACGATTAAAGAGTTTGACTCCGCAAGGAGATAAGGTATACATGTCTCAAGGAAAAGTCAAATCGCTGCTAATATTCAGACTATGTGTTGAGTTTGGGTTGACTGCATGACGCGGCAAAATGATGAGGAAATATGTGTAAAGCGTAAAAGCTTCTTTAGTGAAAAAGCCAAAGGAAGAGGAGAGTCTTTGGTGGAAAAAGATAAGACTTTCTGATGAAAGGGACAAGAAGAAGTTCGAAGTGCTGAACGACAACATGAGAGAATCGCTTTGGGGAAGAGGAACCTAGAAGTAGAGAAGGAAGGAATTGAGGTTAAGTATATGTGTCTTTGTTAGGCATTGAGGCCACTCAAAGATTAGTTCTGAGTGACTCTGTAACCAAGCTAAGGAGGTAAGACAGAATGTTTCAATAGTGTTACATGGTGCGTTGGCTATGAAAGTTTCTATTACGCACTTAACGAAGCCGATTGGTAAGCGAATCGCTCCTTAGCACAAATGGCTCAGCTTAAAAAGTAGGTGAGTGTAATACCCGGAATGGCAAGGTCAAGCTAGAGGATTAAAGATTTAAGTGAGAAAAAAAGTAACCCCAAACACGGATCGCTAAGAGAGGCAGTTAGGCGATTGAGGTGAAGAACTAAGGTGTGTTAAGAAAGCCTGCCATTAAACCAATGAGAGTAAAAGTTGTTGTACTCCGTTGCGTTGTGTTAAGAAAACCTGACATTAAGCCTTCCGTTTAAGTATTCGTTGAAAAGTTGTTTCGCTTACTAAGCAGTCAGCGTTATCAGGAGGTGTGATATACATTTTGGGCTTAAGCGACATGCCTCCCCTTAGTCACAGAGAGCGACTTTGCGGTGGGGCATGATAATTTTTTTTTCGAAATTTAGTACATAATACAAAACTCTTGTGCTAATGTAAAATGCACATCATTTTAATCTAGAGACATAAATAGTTAGTACAAGAGACCTAAATAAATTATCAAGTGTAGTCTTTGTTGGAAAGAAACTTTTCATGTTGGAGGAAATTTAAAAGAGAGAATTGAGAAAAGTGGCTACTTGTGTTATTAGATACGTTTAATTTTTGGCTTGACACAAGTGCTATAACATCCCTCCTATTTGTACTAGTTGATACTACTAAATATATGGCTATAATTTTGCATACACATTTTTAATCTAATGGGTACACATGTCTCTCTAGAATATTATTTATAATTTCTAGATATATTTCTTCAATTTTCTCTACATGTCTCCTACAATTCATTTTTCTAGAAATATTATATTAATACTTCTAGAAATTTGATTTTGATTGGAAAATCAAGTTTATTTTTCTAACGGTTTAAAATTATTATCAACTTAGGGCTGTACCGATGTAGCTTGGGTGACATTGTTCGGGTGACAGGATTCACAAACAAAGCCCCAAAAATTAGTTTTGTACGAAGGAAAAATGTGGTTCTAAACCTTGAGTATGAGAAAACAAATGAAACGGATCTTCGTATGGGTGTAGAAAATGCTGGTGGTGTACTGAAGCCATTTGGGGCAACAATTGTGGACTACACAAGCTATGCTGATACCTCAACAATTCCTGGACACTATGTGTTGTACTGGGAGTTATTAATTGATGGTAATGACCACAACACTCAAACCAATCATTTCATTCCATCTTCAGTGTTTAACGATTGTTGCTTTGCCATCGAAGAATCATTTACTACATTCTACCGTATAAAACGATCGCATGAAAAGACTATCAACCCATTGGAGATTAGAATCGTAAAGAGTGGGACGTTTGAGAAGCTTATGAAGCTGGCCATCAATGGAGGTGCTTCAATGAATCAATACAAGACACCTCGATCTCTAAATTCTAACCAGATTCATTTCATCCAGCTTTTGGAGTCAAACGTTGTTTGCAGTTATTTTAGTCAGAAAAACCCCAGAGTTGGATTACGTTAGTCACCACCGTGCTCGTTTTTCTTTCCCCTACTGCACAGTATTTGTACTTTTTCACCACCGTGCTCGTTGCTCTGCTAGTTTTAACTTTGATGCTTTTCCTTTTCCATC

mRNA sequence

ATGCCGTCACATGTTGAAGAGAGTTGGTTGGATGAGAGAGATAAGGAAGTACTACAACACATTCAGCATATCACAAGCAATGCTGGCGAAATCCAACGACAAATTCTAAGTGAAATTCTGTCAACGAATGCTAATGTTGAATATTTACAACAACATGGCCTTCATGCCTCTACTGGTTCTTCAACCTTCAAAAAACTTATACCTCTTGTCTCTTACGAACAATTAAAACCTTATATAACTCGTATTGCAGAAGGTGATGATTCCCCAATCCTATGCTCAAACCCCATTACAGCGTTCTTTCTAAGCTCTGGGACATCTGGGGGAGAACCCAAGTTAGTTCCAATATATGAAAAAGAGTTTGAGAGAAGGTTGTCTTTTTTCAACTATTTGATGGCAAGGACGAAAGAATTGTTTCCAAATATCAATTGGCACAAAGGTAAAGCTATGAACTTTCACTTTGCAAAGCCTGATCATAAGACAAAAGCTGGAATTCTTGTTCATACTGTATTTTCAAGACTGCTCAAAAGATCACTAAATCTCAAAAGTGTAGAAAGTGGGAATAACGCAATCCCTGATGACATTCTTCGATGCACGAACACATACCAAAGCTTATACTGTCAGCTTCTTTGTGGGCTCTACCAAAACGATCTAGTTTTTCAAGTTGGTGCAGTTTTGGCTTCTGGGTTGATTCACGTTTTTAAGTTCCTTGAAAATCATTGGGTTGATTTGGTTAGTGATATACGTAGAGGAAGTATTAATAACCCCAAAATTACAGACTTATCACTGAGAGAATCAGTTATGAAGATTCTTGTAAAACCCAATCCTCAACTTGCAGATTTGATTGAAACTGAATGTAGCAAAGGAAAATGGAAAGGAATTGTTCCAAAATTGTGGCCAAACACTAAATATATTAAGGCTATTGCCACAGGAAGCTTGTCACAATACATTCCATTGCTAAATTACTACACAAACAACCTCCCTATATTTTCTGACCATTATGGTTCTACTGAATGTTTCCTTGGCTTGAATCTGGATCCAATATGTGACCCTAATGAAACCTCCTACACTTTGATCCCTACCATGGCGTATTTCGAGTTCTTACCAATAGATACCACAAATATCAATGGCGAAGTGACCCAAGAATTAGTTGATCTTGTTGATGTCAAGTTAGGACAAGAATATGAGCTCGTCATCACCACTTTCGCTGGGCTGTACCGATGTAGCTTGGGTGACATTGTTCGGGTGACAGGATTCACAAACAAAGCCCCAAAAATTAGTTTTGTACGAAGGAAAAATGTGGTTCTAAACCTTGAGTATGAGAAAACAAATGAAACGGATCTTCGTATGGGTGTAGAAAATGCTGGTGGTGTACTGAAGCCATTTGGGGCAACAATTGTGGACTACACAAGCTATGCTGATACCTCAACAATTCCTGGACACTATGTGTTGTACTGGGAGTTATTAATTGATGGTAATGACCACAACACTCAAACCAATCATTTCATTCCATCTTCAGTGTTTAACGATTGTTGCTTTGCCATCGAAGAATCATTTACTACATTCTACCGTATAAAACGATCGCATGAAAAGACTATCAACCCATTGGAGATTAGAATCGTAAAGAGTGGGACGTTTGAGAAGCTTATGAAGCTGGCCATCAATGGAGGTGCTTCAATGAATCAATACAAGACACCTCGATCTCTAAATTCTAACCAGATTCATTTCATCCAGCTTTTGGAGTCAAACGTTGTTTGCAGTTATTTTAGTCAGAAAAACCCCAGAGTTGGATTACGTTAG

Coding sequence (CDS)

ATGCCGTCACATGTTGAAGAGAGTTGGTTGGATGAGAGAGATAAGGAAGTACTACAACACATTCAGCATATCACAAGCAATGCTGGCGAAATCCAACGACAAATTCTAAGTGAAATTCTGTCAACGAATGCTAATGTTGAATATTTACAACAACATGGCCTTCATGCCTCTACTGGTTCTTCAACCTTCAAAAAACTTATACCTCTTGTCTCTTACGAACAATTAAAACCTTATATAACTCGTATTGCAGAAGGTGATGATTCCCCAATCCTATGCTCAAACCCCATTACAGCGTTCTTTCTAAGCTCTGGGACATCTGGGGGAGAACCCAAGTTAGTTCCAATATATGAAAAAGAGTTTGAGAGAAGGTTGTCTTTTTTCAACTATTTGATGGCAAGGACGAAAGAATTGTTTCCAAATATCAATTGGCACAAAGGTAAAGCTATGAACTTTCACTTTGCAAAGCCTGATCATAAGACAAAAGCTGGAATTCTTGTTCATACTGTATTTTCAAGACTGCTCAAAAGATCACTAAATCTCAAAAGTGTAGAAAGTGGGAATAACGCAATCCCTGATGACATTCTTCGATGCACGAACACATACCAAAGCTTATACTGTCAGCTTCTTTGTGGGCTCTACCAAAACGATCTAGTTTTTCAAGTTGGTGCAGTTTTGGCTTCTGGGTTGATTCACGTTTTTAAGTTCCTTGAAAATCATTGGGTTGATTTGGTTAGTGATATACGTAGAGGAAGTATTAATAACCCCAAAATTACAGACTTATCACTGAGAGAATCAGTTATGAAGATTCTTGTAAAACCCAATCCTCAACTTGCAGATTTGATTGAAACTGAATGTAGCAAAGGAAAATGGAAAGGAATTGTTCCAAAATTGTGGCCAAACACTAAATATATTAAGGCTATTGCCACAGGAAGCTTGTCACAATACATTCCATTGCTAAATTACTACACAAACAACCTCCCTATATTTTCTGACCATTATGGTTCTACTGAATGTTTCCTTGGCTTGAATCTGGATCCAATATGTGACCCTAATGAAACCTCCTACACTTTGATCCCTACCATGGCGTATTTCGAGTTCTTACCAATAGATACCACAAATATCAATGGCGAAGTGACCCAAGAATTAGTTGATCTTGTTGATGTCAAGTTAGGACAAGAATATGAGCTCGTCATCACCACTTTCGCTGGGCTGTACCGATGTAGCTTGGGTGACATTGTTCGGGTGACAGGATTCACAAACAAAGCCCCAAAAATTAGTTTTGTACGAAGGAAAAATGTGGTTCTAAACCTTGAGTATGAGAAAACAAATGAAACGGATCTTCGTATGGGTGTAGAAAATGCTGGTGGTGTACTGAAGCCATTTGGGGCAACAATTGTGGACTACACAAGCTATGCTGATACCTCAACAATTCCTGGACACTATGTGTTGTACTGGGAGTTATTAATTGATGGTAATGACCACAACACTCAAACCAATCATTTCATTCCATCTTCAGTGTTTAACGATTGTTGCTTTGCCATCGAAGAATCATTTACTACATTCTACCGTATAAAACGATCGCATGAAAAGACTATCAACCCATTGGAGATTAGAATCGTAAAGAGTGGGACGTTTGAGAAGCTTATGAAGCTGGCCATCAATGGAGGTGCTTCAATGAATCAATACAAGACACCTCGATCTCTAAATTCTAACCAGATTCATTTCATCCAGCTTTTGGAGTCAAACGTTGTTTGCAGTTATTTTAGTCAGAAAAACCCCAGAGTTGGATTACGTTAG
BLAST of CSPI02G07750 vs. Swiss-Prot
Match: GH35_ARATH (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 7.3e-173
Identity = 312/598 (52.17%), Postives = 416/598 (69.57%), Query Frame = 1

Query: 10  LDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSSTFKKLIPL 69
           LD+++K+ LQ I+ +TSNA ++QRQ+L EIL+ NA+VEYL++H L+  T   TFK ++P+
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 70  VSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRLSFFNY 129
           ++YE ++P I RIA GD SPIL S PI+ F  SSGTSGGE KL+P  E+E +RR   ++ 
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 130 LMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNLKSVESG--N 189
           LM    +  P +    GK M F F K + KT  G+    V +   K S   +       N
Sbjct: 137 LMPVMSQFVPGLE--NGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTN 196

Query: 190 NAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVDLVSDI 249
              P++ + C+++YQS+Y Q+LCGL Q+  V +VGAV ASG I   KFLE HW++LV DI
Sbjct: 197 YTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDI 256

Query: 250 RRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTKYIKAI 309
           R G++++  ITD S+RE+V KIL KP+P+LAD +E EC K  W+GI+ +LWPNTKY+  I
Sbjct: 257 RTGTLSS-LITDPSVREAVAKIL-KPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVI 316

Query: 310 ATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMAYFEFL 369
            TG++SQYIP L+YY+N LP+    Y S+EC+ G+NL P+C P+E SYTLIP+MAYFEFL
Sbjct: 317 VTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFL 376

Query: 370 PIDTTN-----------INGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVT 429
           P+   N           +  +  QELVDLVDVKLGQEYELV+TT+AGL R  +GD++RVT
Sbjct: 377 PVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVT 436

Query: 430 GFTNKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTST 489
           GF NKAP+ SF+ RKNVVL+++ +KT+E +L+  V+NA   L PF A++ +YTSYADTS+
Sbjct: 437 GFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSS 496

Query: 490 IPGHYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLE 549
           IPGHYVL+WEL +DGN         IP SVF DCC A+EESF T YR  R  +K+I PLE
Sbjct: 497 IPGHYVLFWELCLDGNTP-------IPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLE 556

Query: 550 IRIVKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPR 595
           I+IV+ GTF+KLM  AI+ GAS+NQYKTPR +    I  I+LL S VV SYFS K P+
Sbjct: 557 IKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPI--IELLNSRVVDSYFSPKCPK 601

BLAST of CSPI02G07750 vs. Swiss-Prot
Match: GH36_ARATH (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 5.8e-170
Identity = 308/603 (51.08%), Postives = 414/603 (68.66%), Query Frame = 1

Query: 5   VEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSSTFK 64
           V +  L E++K  LQ I+ +T+NA ++QR++L EILS NA+VEYL++HGL   T   TFK
Sbjct: 12  VSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFK 71

Query: 65  KLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRL 124
            ++P+V+YE ++P I RIA GD S +LCSNPI+ F  SSGTSGGE KL+P  E+E +RR 
Sbjct: 72  HIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRS 131

Query: 125 SFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRS--LNLKS 184
             ++ LM    +  P ++  KGK M F F K + KT  G+    V +   K S   N   
Sbjct: 132 LLYSLLMPVMDQFVPGLD--KGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPY 191

Query: 185 VESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVD 244
               N   P+  + C+++YQS+Y Q+LCGL Q+  V +VGAV ASG I   KFLE HW +
Sbjct: 192 DPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPE 251

Query: 245 LVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTK 304
           L  DIR G++++ +ITD S+RE+V +IL KP+P+LAD +E+EC K  W+GI+ +LWPNTK
Sbjct: 252 LARDIRTGTLSS-EITDSSVREAVGEIL-KPDPKLADFVESECRKTSWQGIITRLWPNTK 311

Query: 305 YIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMA 364
           Y+  I TG++SQYIP L+YY+N LP+    Y S+EC+ G+NL P+C P+E SYTLIP MA
Sbjct: 312 YVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMA 371

Query: 365 YFEFLPIDTTN-----------INGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGD 424
           YFEFLP+   +           +  +  QELVDLVDVKLGQEYELV+TT+AGLYR  +GD
Sbjct: 372 YFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGD 431

Query: 425 IVRVTGFTNKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSY 484
           ++ V GF N AP+ SF+ RKNVVL+++ +KT+E +L+  V+NA   L PF A++ +YTSY
Sbjct: 432 VLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSY 491

Query: 485 ADTSTIPGHYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKT 544
           ADTS+IPGHYVL+WEL ++GN         IP SVF DCC  IEES  + YR  R  +K+
Sbjct: 492 ADTSSIPGHYVLFWELCLNGNTP-------IPPSVFEDCCLTIEESLNSVYRQGRVSDKS 551

Query: 545 INPLEIRIVKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQK 595
           I PLEI++V+SGTF+KLM  AI+ GAS+NQYKTPR +    I  I+LL S VV SYFS K
Sbjct: 552 IGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPI--IELLNSRVVDSYFSPK 601

BLAST of CSPI02G07750 vs. Swiss-Prot
Match: GH31_ORYSJ (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 1.7e-153
Identity = 274/583 (47.00%), Postives = 384/583 (65.87%), Query Frame = 1

Query: 16  EVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS-STFKKLIPLVSYEQ 75
           E L+ I+H+T+NAG++QR++L EIL+ NA  EYL+++G+  S      F++L+PLV+YE 
Sbjct: 22  EALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEG 81

Query: 76  LKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRLSFFNYLMART 135
           L+P I RIA GD SPI    PI+ F  SSGTSGGE KL+P    E  RR   ++ LM   
Sbjct: 82  LQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVM 141

Query: 136 KELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNLKSVESGNNAI--PD 195
            +    ++  KGKAM   F K + +T  G+    V +   +    L        +   PD
Sbjct: 142 SQSVSGLD--KGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPD 201

Query: 196 DILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVDLVSDIRRGSI 255
           + + C ++YQS+Y QLLCGL     V +VGAV ASG +    FLE HW  L  DIR G +
Sbjct: 202 EAILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGEL 261

Query: 256 NNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTKYIKAIATGSL 315
           + P+ITD  +R++V ++L + +P LAD IE EC++  W+GI+ +LWP TKYI  I TG++
Sbjct: 262 D-PEITDRVVRDAVGRVL-RADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTM 321

Query: 316 SQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMAYFEFLPIDTT 375
           SQYIP L +Y   LP+    Y S+EC+ GLNL+P+C P++ +YTLIPTM Y+EFLP++  
Sbjct: 322 SQYIPTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCN 381

Query: 376 NINGEVT-QELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTNKAPKISFVRRK 435
           N   E + ++LVDLVDVKLG EYELV+TT++GLYR  +GD++RV GF NKAP  SFVRR+
Sbjct: 382 NATAEASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVRRQ 441

Query: 436 NVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGHYVLYWELLIDG 495
           NV L+++ +KT+ET+L   V  A   L PFGA++V+YTSYAD +TIPGHYVL+WEL    
Sbjct: 442 NVALSVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWELRAGS 501

Query: 496 NDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIVKSGTFEKLMKL 555
                     +P+SVF +CC ++EE+  + YR  R+ +++I PLEIR+V  GTF+KLM  
Sbjct: 502 T--------AVPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDY 561

Query: 556 AINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPR 595
           AI+ GAS+NQYK PR +    +  ++LL++ V   YFS K P+
Sbjct: 562 AISRGASINQYKAPRCVRPGPV--VELLDARVQGKYFSPKCPK 590

BLAST of CSPI02G07750 vs. Swiss-Prot
Match: GH34_ORYSJ (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 8.7e-150
Identity = 272/590 (46.10%), Postives = 380/590 (64.41%), Query Frame = 1

Query: 15  KEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGL--HASTGSSTFKKLIPLVSY 74
           +E L+HI+ +T NAG+ QR++L EIL+ NA  EYL++ G+   A      F++L PLV+Y
Sbjct: 24  REKLEHIERVTRNAGQEQRRVLEEILAQNAQAEYLRRLGVPGDAPGADEAFRRLAPLVTY 83

Query: 75  EQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRLSFFNYLMA 134
           E + P + RIA GD SPIL   P++ F  SSGTSGGE KL+P  E+E ERR   ++ LM 
Sbjct: 84  EDILPDVLRIANGDTSPILSGKPVSEFLTSSGTSGGERKLMPTIEEEMERRSGLYSLLMP 143

Query: 135 RTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNLKSVESGNNAI-- 194
                 P ++  KGKAM  +F K + +T  G+    V +   +    L+           
Sbjct: 144 VMSRQVPGLD--KGKAMYLYFVKSEWRTPGGLPARPVLTSFYRSRYFLERPHDPYTVYTS 203

Query: 195 PDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVDLVSDIRRG 254
           PD+ + C + YQS+Y QL+CGL     V +VGAV ASG +   +FLE HW  L  DIR G
Sbjct: 204 PDEAVLCEDAYQSMYAQLICGLVHRADVLRVGAVFASGFLRAIRFLEKHWPSLCRDIRAG 263

Query: 255 SINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTKYIKAIATG 314
            ++   +TD ++R +V ++L   +P LAD IE EC++  W+GI+ ++WP+TKYI  I TG
Sbjct: 264 ELDGG-VTDPAVRGAVGRVLRGADPALADAIEAECARPSWQGIIRRVWPSTKYIDVIVTG 323

Query: 315 SLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMAYFEFLPID 374
           +++QYIP L +Y   LP+    Y S+EC+ GLNL+P+C P+E +YTLIPTM YFEFLP++
Sbjct: 324 AMAQYIPTLEFYGGGLPLACTMYASSECYFGLNLNPMCKPSEVAYTLIPTMCYFEFLPVN 383

Query: 375 TTNINGEVTQE-----LVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTNKAPKI 434
            +  N     E     LVDLVDVKLG EYELV+TT++GLYR  +GD++RV GF N AP  
Sbjct: 384 -SGANDVAAPEPDHRGLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNAAPMF 443

Query: 435 SFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADT-STIPGHYVLY 494
           +FVRRKNV L+++ +KT+E +L   V  A   L PFGA++V+YTSYADT +TIPGHYVL+
Sbjct: 444 AFVRRKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASLVEYTSYADTATTIPGHYVLF 503

Query: 495 WELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIVKSGT 554
           WEL       +      +P+SVF DCC A+EE   + YR  R+ +++I PLEIR+V  GT
Sbjct: 504 WEL------RSPAGGTPVPASVFEDCCLAVEEGLNSVYRQCRAADRSIGPLEIRVVADGT 563

Query: 555 FEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPR 595
           F+KLM  A++ GAS+NQYK PR +    +  ++LL+  V  +YFS K P+
Sbjct: 564 FDKLMDYALSRGASINQYKAPRCVRPGPV--VELLDGRVQATYFSPKCPK 601

BLAST of CSPI02G07750 vs. Swiss-Prot
Match: GH38_ORYSI (Probable indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica GN=GH3.8 PE=2 SV=2)

HSP 1 Score: 525.0 bits (1351), Expect = 1.1e-147
Identity = 271/585 (46.32%), Postives = 376/585 (64.27%), Query Frame = 1

Query: 12  ERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSSTFKKLIPLVS 71
           E D E L+ I  +T+N   +Q ++L EIL  NA  EYL + GL  +T  + F+  +P+VS
Sbjct: 24  EGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVS 83

Query: 72  YEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRLSFFNYLM 131
           Y+ L+PYI RIA GD SPIL ++P++ F  SSGTS GE KL+P    E +RR   ++ LM
Sbjct: 84  YDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLM 143

Query: 132 ARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRS--LNLKSVESGNNA 191
                  P ++  KGK + F F K + KT  G+    V +   K     N       N  
Sbjct: 144 PVMNLYVPGLD--KGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYT 203

Query: 192 IPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVDLVSDIRR 251
            P   + C + +QS+Y Q++CGL Q + V ++GAV ASGL+   +FL+ +W  L  DI  
Sbjct: 204 SPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIES 263

Query: 252 GSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTKYIKAIAT 311
           G +  P++TD S+RE+V  IL+ P+P+LA LI  ECSKG W GI+ ++WPNTKY+  I T
Sbjct: 264 GELT-PRVTDPSVREAVAAILL-PDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVT 323

Query: 312 GSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMAYFEFLPI 371
           G+++QYIP L +Y+  LP+    Y S+EC+ GLNL P+CDP+E SYT++P M YFEFLP+
Sbjct: 324 GAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPV 383

Query: 372 DTTN-INGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTNKAPKISFV 431
           D T   +G+ TQ LVDL  V++G+EYELVITT+AGL R  +GD++RVTGF N AP+  FV
Sbjct: 384 DETGAASGDATQ-LVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNAAPQFRFV 443

Query: 432 RRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGHYVLYWELL 491
           RRKNV+L++E +KT+E +L+  VE A  +L+P GA++V+YTS A T  IPGHYV+YWELL
Sbjct: 444 RRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELL 503

Query: 492 IDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIVKSGTFEKL 551
             G          + +     CC  +EE+  T YR  R  + +I PLEIR+V+ GTFE+L
Sbjct: 504 TKGAGATV-----VDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEEL 563

Query: 552 MKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNP 594
           M  AI+ GAS+NQYK PR +    I  ++LL+S VV S+FS   P
Sbjct: 564 MDYAISRGASINQYKVPRCVTFPPI--VELLDSRVVSSHFSPALP 596

BLAST of CSPI02G07750 vs. TrEMBL
Match: A0A0A0LHA0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G081190 PE=4 SV=1)

HSP 1 Score: 1216.1 bits (3145), Expect = 0.0e+00
Identity = 595/597 (99.66%), Postives = 595/597 (99.66%), Query Frame = 1

Query: 1   MPSHVEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS 60
           MPSHVEESWLDERDKE LQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS
Sbjct: 1   MPSHVEESWLDERDKEALQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS 60

Query: 61  STFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEF 120
           STFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEF
Sbjct: 61  STFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEF 120

Query: 121 ERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNL 180
           ERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNL
Sbjct: 121 ERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNL 180

Query: 181 KSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHW 240
           KSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHW
Sbjct: 181 KSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHW 240

Query: 241 VDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPN 300
           VDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPN
Sbjct: 241 VDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPN 300

Query: 301 TKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPT 360
           TKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPT
Sbjct: 301 TKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPT 360

Query: 361 MAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTN 420
           MAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTN
Sbjct: 361 MAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTN 420

Query: 421 KAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGH 480
           KAPK SFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGH
Sbjct: 421 KAPKFSFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGH 480

Query: 481 YVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIV 540
           YVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIV
Sbjct: 481 YVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIV 540

Query: 541 KSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL 598
           KSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL
Sbjct: 541 KSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL 597

BLAST of CSPI02G07750 vs. TrEMBL
Match: A0A0A0KAF9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G125240 PE=4 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 1.0e-250
Identity = 426/592 (71.96%), Postives = 495/592 (83.61%), Query Frame = 1

Query: 5   VEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSSTFK 64
           VEE WLDE++KE LQ+I+ IT+ A EIQRQIL+EILSTNANVEYLQQHGL   T S TFK
Sbjct: 4   VEEKWLDEKEKEALQYIEDITTKADEIQRQILNEILSTNANVEYLQQHGLVVPTDSPTFK 63

Query: 65  KLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRL 124
           KLIPLV YEQL+PYI RIA+GDDS ILCSNPIT FF SSGTSGGE KL+P+YE+EF RRL
Sbjct: 64  KLIPLVCYEQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGGEHKLIPMYEQEFVRRL 123

Query: 125 SFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNLKSVE 184
           SFF+Y+M R K+LFP+INWHK K +NFHFAKP+ KTK GI+V ++F+ L KRS NL+S+ 
Sbjct: 124 SFFSYIMPRMKQLFPDINWHKVKGLNFHFAKPEFKTKGGIIVRSIFTNLHKRSSNLESMP 183

Query: 185 SGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVDLV 244
           SGNN  PDDI+ CT++YQSLYCQLLCGLYQN++VF+V A+ AS LIHVFKFLENHWVDL 
Sbjct: 184 SGNNTSPDDIILCTDSYQSLYCQLLCGLYQNEVVFRVSALFASTLIHVFKFLENHWVDLA 243

Query: 245 SDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTKYI 304
           +DIR  ++ NPKIT+ S+RES+MKI+VKPNP++ADLIE EC KG+W+GI+ +LWPN KYI
Sbjct: 244 TDIRTRTV-NPKITNSSVRESLMKIIVKPNPEVADLIENECRKGRWEGIITRLWPNAKYI 303

Query: 305 KAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMAYF 364
            AI TGS+SQYIPLLNYYTNNLPI SDHYGS+ECFLGLNLDP+C+ +E SYTLIPTMAYF
Sbjct: 304 NAIVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNHDEVSYTLIPTMAYF 363

Query: 365 EFLPIDTTN-INGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTNKAP 424
           EFLPID  N  NGEV Q+LVDLVDVKLG+EYELVITTFAGLYR  +GDIVRVTGF NKAP
Sbjct: 364 EFLPIDMINDPNGEVNQQLVDLVDVKLGREYELVITTFAGLYRYCMGDIVRVTGFKNKAP 423

Query: 425 KISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGHYVL 484
              FVRRKNVVLN+  EKT+E  L   VE  G V+K FGA IVDYT+YAD STIPGHYVL
Sbjct: 424 SFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKIVDYTTYADLSTIPGHYVL 483

Query: 485 YWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIVKSG 544
           YWEL +D  D   Q ++ IPSSVF DCC  IE S    YR+ RSHEK INPLEI+IVK+G
Sbjct: 484 YWELTMD--DLKEQNSNDIPSSVFEDCCLDIENSLNLLYRLARSHEKCINPLEIKIVKAG 543

Query: 545 TFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRV 596
           TFEKLM+LA++ GAS+ QYKTPR LNS+QIH IQLLESNVV +YFS+K P +
Sbjct: 544 TFEKLMQLALDRGASITQYKTPRCLNSSQIHIIQLLESNVVSNYFSRKYPNL 592

BLAST of CSPI02G07750 vs. TrEMBL
Match: I1LRV0_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_12G103500 PE=4 SV=2)

HSP 1 Score: 624.0 bits (1608), Expect = 1.9e-175
Identity = 316/596 (53.02%), Postives = 428/596 (71.81%), Query Frame = 1

Query: 8   SWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSSTFKKLI 67
           S ++  +K+ L++I+ +TSNA EIQ+++L+EILS++A+ EYLQ+HGL   T   TFKK++
Sbjct: 25  SIIEHNNKKALKYIEDVTSNADEIQKRVLAEILSSSAHAEYLQRHGLDGRTDRETFKKIM 84

Query: 68  PLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRLSFF 127
           P+V+YE LKP I RIA GD SPILCS PI+ F  SSGTSGGE KL+P  E+E ERR   +
Sbjct: 85  PVVTYEDLKPDIDRIANGDTSPILCSKPISEFLTSSGTSGGERKLMPTIEEELERRSLLY 144

Query: 128 NYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNLKSVES-- 187
           + LM   ++  P ++  KGK M F F K + KT  G+L   V +    RS + K+     
Sbjct: 145 SLLMPVMEQFVPGLD--KGKGMYFLFIKSEAKTPGGLLARPVLTSYY-RSSHFKNKTHCF 204

Query: 188 ---GNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVD 247
               N   P + + C ++YQS+Y Q+LCGL QN+ V +VGAV ASG I   KFLE HWV 
Sbjct: 205 DPYTNYTSPIETILCLDSYQSMYSQMLCGLSQNEHVLRVGAVFASGFIRALKFLEKHWVC 264

Query: 248 LVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTK 307
           L  DIR G+I  P+ITD S+RE++M++L KPNP+LAD IE EC KG WKGI+ +LWPNTK
Sbjct: 265 LCRDIRNGTIG-PEITDSSVREAIMRVL-KPNPKLADFIEGECKKGLWKGIITRLWPNTK 324

Query: 308 YIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMA 367
           Y+  I TG+++QYIP+L+YY+N LP+    Y S+EC+ GLNL+P+CDP+E SYTL+PTMA
Sbjct: 325 YVDVIVTGTMAQYIPMLDYYSNGLPLVCTMYASSECYFGLNLNPLCDPSEVSYTLVPTMA 384

Query: 368 YFEFLPIDTT-----NINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTG 427
           YFEFLP++ T     +I+    + LVDLVDV+LGQEYELV+TT+AGLYR  +GDI+RV G
Sbjct: 385 YFEFLPLNKTKEHANSISYTEQELLVDLVDVELGQEYELVVTTYAGLYRYRVGDILRVAG 444

Query: 428 FTNKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTI 487
           F N AP+ +FV RKNVVL+++ +KT+E +L+  V+N    L  FGA++ +YTS+ADTSTI
Sbjct: 445 FKNNAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNGANHLTLFGASLTEYTSFADTSTI 504

Query: 488 PGHYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEI 547
           PGHYVLYWE+ ++ N++  Q  + IPSSVF +CCFA+E S  + YR  R  E +I PLEI
Sbjct: 505 PGHYVLYWEISMNKNNNIDQNQNPIPSSVFEECCFAVEGSLNSVYRQGRVSE-SIGPLEI 564

Query: 548 RIVKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNP 594
           +IV++GTF+KLM  A++ GAS+NQYKTPR +    I  ++LL+S  V +YFS K P
Sbjct: 565 KIVENGTFDKLMDFALSQGASINQYKTPRCVKYAPI--VELLDSKTVSNYFSPKCP 612

BLAST of CSPI02G07750 vs. TrEMBL
Match: V7AHM5_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_011G153400g PE=4 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 5.5e-175
Identity = 317/607 (52.22%), Postives = 423/607 (69.69%), Query Frame = 1

Query: 1   MPSHVEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS 60
           MP    +  L E+++++L+ I+ +T+NA ++Q+++LSEILS NANVEYL++HGLH  T  
Sbjct: 1   MPEAPRDYNLVEQNEKILEFIEDVTANADKVQKRVLSEILSNNANVEYLKRHGLHGQTDR 60

Query: 61  STFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEF 120
            TFKKL+P+++YE ++P I RIA GD SPILCS PI+ F  SSGTSGGE KL+P  E++ 
Sbjct: 61  ETFKKLLPVITYEDIQPDINRIANGDTSPILCSKPISEFLTSSGTSGGERKLMPTIEEDL 120

Query: 121 ERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSL-- 180
            RR S +  LM       P +   KGK M   F K + KT  GI+   V +   K S   
Sbjct: 121 GRRRSLYGLLMPVMSLFVPGLE--KGKGMYLMFIKSEAKTPGGIVARPVLTSYYKSSYFK 180

Query: 181 NLKSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLEN 240
           +       N   P++ + C ++YQS+Y QLLCGL QN  VF+VGAV ASG I   +FLE 
Sbjct: 181 DRPYDPYTNYTSPNETVLCPDSYQSMYSQLLCGLCQNKEVFRVGAVFASGFIRAIRFLEK 240

Query: 241 HWVDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLW 300
           HW  L +DIR G++N P+ITD S+RE+VMKIL KP+P+LADLI+TECSK  W+GI+ +LW
Sbjct: 241 HWSLLCNDIRTGTVN-PQITDTSVREAVMKIL-KPDPKLADLIQTECSKNSWQGIITRLW 300

Query: 301 PNTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLI 360
           PNT+Y+  I TG++SQYIP L+YY+N LP+    Y S+EC+ G+NL+P+C P+E SYTLI
Sbjct: 301 PNTRYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVSYTLI 360

Query: 361 PTMAYFEFLPIDTTN-----------INGEVTQELVDLVDVKLGQEYELVITTFAGLYRC 420
           PTM YFEFLP++ +N           +N +  QELV+LVDVKLGQEYELV+TT+AGLYR 
Sbjct: 361 PTMCYFEFLPVNRSNGVNDPLHTSRSLNEKEQQELVELVDVKLGQEYELVVTTYAGLYRY 420

Query: 421 SLGDIVRVTGFTNKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVD 480
            +GD++RV GF NKAP+ +FV RKNVVL+++ +KT+E +L+  ++NA   L PF A++ D
Sbjct: 421 RVGDVLRVAGFKNKAPQFNFVCRKNVVLSIDSDKTDEVELQNAMKNAVTHLVPFDASVSD 480

Query: 481 YTSYADTSTIPGHYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRS 540
           YTSYADT+TIPGHYVLYWEL + G+         IP  VF DCC  IEES  + YR  R 
Sbjct: 481 YTSYADTTTIPGHYVLYWELALKGSTP-------IPPCVFEDCCLTIEESLNSVYRQGRV 540

Query: 541 HEKTINPLEIRIVKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSY 595
            +K+I PLEI+IV+ GTF+KLM  AI+ GAS+NQYKTPR +    +  ++LL S VV  Y
Sbjct: 541 SDKSIGPLEIKIVEQGTFDKLMDYAISLGASINQYKTPRCVKFAPV--VELLNSRVVEKY 594

BLAST of CSPI02G07750 vs. TrEMBL
Match: A0A0S3S9C7_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.06G035000 PE=4 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 9.3e-175
Identity = 318/607 (52.39%), Postives = 423/607 (69.69%), Query Frame = 1

Query: 1   MPSHVEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS 60
           MP    +  L E+++++L  I+ +T+NA ++Q+++LSEILS+NANVEYL++HGLH  T  
Sbjct: 1   MPEAPRDYNLVEQNEKILDFIEDVTANADQVQKRVLSEILSSNANVEYLKRHGLHGQTDR 60

Query: 61  STFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEF 120
            TFKKL+P+++YE ++P I RIA GD SPILCS PI+ F  SSGTSGGE KL+P  E++ 
Sbjct: 61  ETFKKLLPVITYEDIQPDINRIANGDTSPILCSKPISEFLTSSGTSGGERKLMPTIEEDL 120

Query: 121 ERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSL-- 180
            RR S +  LM       P +   KGK M   F K + KT  GI+   V +   K S   
Sbjct: 121 GRRSSLYGLLMPVMSLFVPGLE--KGKGMYLMFIKSEAKTPGGIVARPVLTSYYKSSYFK 180

Query: 181 NLKSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLEN 240
           +       N   P++ + C ++YQS+Y QLLCGL QN  VF+VGAV ASG I   +FLE 
Sbjct: 181 DRPYDPYTNYTSPNETVLCPDSYQSMYSQLLCGLCQNKEVFRVGAVFASGFIRAIRFLEK 240

Query: 241 HWVDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLW 300
           HW  L +DIR G+IN+ +ITD S+RE+VMKIL KP+P+LADLI+TECSK  W+GI+ +LW
Sbjct: 241 HWSLLCNDIRTGTINS-QITDTSVREAVMKIL-KPDPKLADLIQTECSKSSWQGIITRLW 300

Query: 301 PNTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLI 360
           PNTKY+  I TG++SQYIP L+YY+N LP+    Y S+EC+ G+NL+P+C P+E SYTLI
Sbjct: 301 PNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVSYTLI 360

Query: 361 PTMAYFEFLPIDTTN-----------INGEVTQELVDLVDVKLGQEYELVITTFAGLYRC 420
           PTM YFEFLP++ +N           +N +  QELV+LVDVKLGQEYELV+TT+AGLYR 
Sbjct: 361 PTMCYFEFLPVNRSNGVNDPLHTPRSLNEKQQQELVELVDVKLGQEYELVVTTYAGLYRY 420

Query: 421 SLGDIVRVTGFTNKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVD 480
            +GD++RV GF NKAP+ +FV RKNVVL+++ +KT+E +L+  ++NA   L PF A++ D
Sbjct: 421 RVGDVLRVAGFKNKAPQFNFVCRKNVVLSIDSDKTDEVELQNAMKNAVTHLVPFDASVSD 480

Query: 481 YTSYADTSTIPGHYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRS 540
           YTSYADT+TIPGHYVLYWEL + G+         IP  VF DCC  IEES  + YR  R 
Sbjct: 481 YTSYADTTTIPGHYVLYWELALKGSTP-------IPPCVFEDCCLTIEESLNSVYRQGRV 540

Query: 541 HEKTINPLEIRIVKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSY 595
            +K+I PLEI+IV+ GTF+KLM  AI+ GAS+NQYKTPR +    +  ++LL S VV  Y
Sbjct: 541 SDKSIGPLEIKIVEQGTFDKLMDYAISLGASINQYKTPRCVKFAPV--VELLNSRVVEKY 594

BLAST of CSPI02G07750 vs. TAIR10
Match: AT4G27260.1 (AT4G27260.1 Auxin-responsive GH3 family protein)

HSP 1 Score: 608.6 bits (1568), Expect = 4.1e-174
Identity = 312/598 (52.17%), Postives = 416/598 (69.57%), Query Frame = 1

Query: 10  LDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSSTFKKLIPL 69
           LD+++K+ LQ I+ +TSNA ++QRQ+L EIL+ NA+VEYL++H L+  T   TFK ++P+
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 70  VSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRLSFFNY 129
           ++YE ++P I RIA GD SPIL S PI+ F  SSGTSGGE KL+P  E+E +RR   ++ 
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 130 LMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNLKSVESG--N 189
           LM    +  P +    GK M F F K + KT  G+    V +   K S   +       N
Sbjct: 137 LMPVMSQFVPGLE--NGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTN 196

Query: 190 NAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVDLVSDI 249
              P++ + C+++YQS+Y Q+LCGL Q+  V +VGAV ASG I   KFLE HW++LV DI
Sbjct: 197 YTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDI 256

Query: 250 RRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTKYIKAI 309
           R G++++  ITD S+RE+V KIL KP+P+LAD +E EC K  W+GI+ +LWPNTKY+  I
Sbjct: 257 RTGTLSS-LITDPSVREAVAKIL-KPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVI 316

Query: 310 ATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMAYFEFL 369
            TG++SQYIP L+YY+N LP+    Y S+EC+ G+NL P+C P+E SYTLIP+MAYFEFL
Sbjct: 317 VTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFL 376

Query: 370 PIDTTN-----------INGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVT 429
           P+   N           +  +  QELVDLVDVKLGQEYELV+TT+AGL R  +GD++RVT
Sbjct: 377 PVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVT 436

Query: 430 GFTNKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTST 489
           GF NKAP+ SF+ RKNVVL+++ +KT+E +L+  V+NA   L PF A++ +YTSYADTS+
Sbjct: 437 GFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSS 496

Query: 490 IPGHYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLE 549
           IPGHYVL+WEL +DGN         IP SVF DCC A+EESF T YR  R  +K+I PLE
Sbjct: 497 IPGHYVLFWELCLDGNTP-------IPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLE 556

Query: 550 IRIVKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPR 595
           I+IV+ GTF+KLM  AI+ GAS+NQYKTPR +    I  I+LL S VV SYFS K P+
Sbjct: 557 IKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPI--IELLNSRVVDSYFSPKCPK 601

BLAST of CSPI02G07750 vs. TAIR10
Match: AT5G54510.1 (AT5G54510.1 Auxin-responsive GH3 family protein)

HSP 1 Score: 599.0 bits (1543), Expect = 3.3e-171
Identity = 308/603 (51.08%), Postives = 414/603 (68.66%), Query Frame = 1

Query: 5   VEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSSTFK 64
           V +  L E++K  LQ I+ +T+NA ++QR++L EILS NA+VEYL++HGL   T   TFK
Sbjct: 12  VSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFK 71

Query: 65  KLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRL 124
            ++P+V+YE ++P I RIA GD S +LCSNPI+ F  SSGTSGGE KL+P  E+E +RR 
Sbjct: 72  HIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRS 131

Query: 125 SFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRS--LNLKS 184
             ++ LM    +  P ++  KGK M F F K + KT  G+    V +   K S   N   
Sbjct: 132 LLYSLLMPVMDQFVPGLD--KGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPY 191

Query: 185 VESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVD 244
               N   P+  + C+++YQS+Y Q+LCGL Q+  V +VGAV ASG I   KFLE HW +
Sbjct: 192 DPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPE 251

Query: 245 LVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTK 304
           L  DIR G++++ +ITD S+RE+V +IL KP+P+LAD +E+EC K  W+GI+ +LWPNTK
Sbjct: 252 LARDIRTGTLSS-EITDSSVREAVGEIL-KPDPKLADFVESECRKTSWQGIITRLWPNTK 311

Query: 305 YIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMA 364
           Y+  I TG++SQYIP L+YY+N LP+    Y S+EC+ G+NL P+C P+E SYTLIP MA
Sbjct: 312 YVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMA 371

Query: 365 YFEFLPIDTTN-----------INGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGD 424
           YFEFLP+   +           +  +  QELVDLVDVKLGQEYELV+TT+AGLYR  +GD
Sbjct: 372 YFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGD 431

Query: 425 IVRVTGFTNKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSY 484
           ++ V GF N AP+ SF+ RKNVVL+++ +KT+E +L+  V+NA   L PF A++ +YTSY
Sbjct: 432 VLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSY 491

Query: 485 ADTSTIPGHYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKT 544
           ADTS+IPGHYVL+WEL ++GN         IP SVF DCC  IEES  + YR  R  +K+
Sbjct: 492 ADTSSIPGHYVLFWELCLNGNTP-------IPPSVFEDCCLTIEESLNSVYRQGRVSDKS 551

Query: 545 INPLEIRIVKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQK 595
           I PLEI++V+SGTF+KLM  AI+ GAS+NQYKTPR +    I  I+LL S VV SYFS K
Sbjct: 552 IGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPI--IELLNSRVVDSYFSPK 601

BLAST of CSPI02G07750 vs. TAIR10
Match: AT4G37390.1 (AT4G37390.1 Auxin-responsive GH3 family protein)

HSP 1 Score: 515.0 bits (1325), Expect = 6.2e-146
Identity = 265/602 (44.02%), Postives = 386/602 (64.12%), Query Frame = 1

Query: 1   MPSHVEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS 60
           + S +  +   E+D + L+ I+ +T N   +Q ++L EIL+ N+N EYL++  L      
Sbjct: 7   LQSRMVSATTSEKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDR 66

Query: 61  STFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEF 120
            TFK  +P+V+YE LKP I RI+ GD SPIL S+PIT F  SSGTS GE KL+P  E++ 
Sbjct: 67  KTFKSKVPVVTYEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDL 126

Query: 121 ERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNL 180
           +RR   ++ LM       P ++  KGK + F F K + KT  G+    V +   K     
Sbjct: 127 DRRQLLYSLLMPVMNLYVPGLD--KGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFK 186

Query: 181 KSVESGNNAI--PDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLEN 240
           +      N    P++ + C+++ QS+Y Q+LCGL     V ++GAV ASGL+    FL+N
Sbjct: 187 RRPYDPYNVYTSPNEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQN 246

Query: 241 HWVDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLW 300
           +W +L  DI  G++++ +I D +++  + KIL KP+ +LA+ +   CS+  W+GI+ K+W
Sbjct: 247 NWKELARDISTGTLSS-RIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIW 306

Query: 301 PNTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLI 360
           PNTKY+  I TG+++QYIP L YY+  LP+    Y S+E + G+NL P+C P+E SYT++
Sbjct: 307 PNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIM 366

Query: 361 PTMAYFEFLPIDTTNINGEVTQE-------LVDLVDVKLGQEYELVITTFAGLYRCSLGD 420
           P MAYFEFLP    N +G+   E       LV+L +V++G+EYELVITT+AGLYR  +GD
Sbjct: 367 PNMAYFEFLP---HNHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGD 426

Query: 421 IVRVTGFTNKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSY 480
           I+RVTGF N AP+  F+RRKNV+L++E +KT+E +L+  VENA  +    G  +++YTSY
Sbjct: 427 ILRVTGFHNSAPQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSY 486

Query: 481 ADTSTIPGHYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKT 540
           A+T TIPGHYV+YWELL  G D   Q+N  +   V   CC  +EES  + YR  R  +K+
Sbjct: 487 AETKTIPGHYVIYWELL--GRD---QSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKS 546

Query: 541 INPLEIRIVKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQK 594
           I PLEIR+V++GTFE+LM  AI+ GAS+NQYK PR ++   I  ++LL+S VV ++FS  
Sbjct: 547 IGPLEIRVVRNGTFEELMDYAISRGASINQYKVPRCVSFTPI--MELLDSRVVSAHFSPS 595

BLAST of CSPI02G07750 vs. TAIR10
Match: AT2G14960.1 (AT2G14960.1 Auxin-responsive GH3 family protein)

HSP 1 Score: 510.8 bits (1314), Expect = 1.2e-144
Identity = 265/585 (45.30%), Postives = 379/585 (64.79%), Query Frame = 1

Query: 12  ERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSSTFKKLIPLVS 71
           E+D + L+ I+ +T NA  +Q  +L+EIL+ NA+ EYL++  L  +T   TFK  IP+++
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 72  YEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRLSFFNYLM 131
           YE L+P I RIA+GD SPIL ++PI+ F  SSGTS GE KL+P  ++E +RR   ++ LM
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 132 ARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNLKSVESGNNAI- 191
                  P ++  KGK M F F K + KT  G+    V +   K            N   
Sbjct: 137 PVMNLYVPGLD--KGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYT 196

Query: 192 -PDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVDLVSDIRR 251
            P++ + C +++QS+Y Q+LCGL     V +VGAV ASGL+   +FL+ HW     DI  
Sbjct: 197 SPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIEL 256

Query: 252 GSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTKYIKAIAT 311
           G +++ +ITD S+R+ +  IL KP+P LA+ I  EC    W+ I+ ++WPNTKY+  I T
Sbjct: 257 GCLDS-EITDPSIRQCMSGIL-KPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVT 316

Query: 312 GSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMAYFEFLPI 371
           G+++QYIP L YY+  LP+    Y S+EC+ GLNL+P+  P+E SYT++P MAYFEF+P+
Sbjct: 317 GAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPL 376

Query: 372 DTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTNKAPKISFVR 431
             T        + V+LVDV +G+EYELV+TT+AGL R  +GDI+RVTGF N AP+  FVR
Sbjct: 377 GGT--------KAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVR 436

Query: 432 RKNVVLNLEYEKTNETDLRMGVENAGGVL-KPFGATIVDYTSYADTSTIPGHYVLYWELL 491
           RKNV+L+++ +KT+E++L+  VENA  +L +  G+ + +YTSYADTSTIPGHYVLYWELL
Sbjct: 437 RKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELL 496

Query: 492 IDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIVKSGTFEKL 551
           +   D   Q +H         CC  +EES  + YR  R  + ++ PLEIR+V++GTFE+L
Sbjct: 497 V--RDGARQPSH----ETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEEL 556

Query: 552 MKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNP 594
           M  AI+ GAS+NQYK PR +N   I  ++LL+S VV ++FS   P
Sbjct: 557 MDYAISRGASINQYKVPRCVNFTPI--VELLDSRVVSAHFSPSLP 581

BLAST of CSPI02G07750 vs. TAIR10
Match: AT5G13360.3 (AT5G13360.3 Auxin-responsive GH3 family protein)

HSP 1 Score: 507.7 bits (1306), Expect = 9.9e-144
Identity = 263/583 (45.11%), Postives = 371/583 (63.64%), Query Frame = 1

Query: 15  KEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSSTFKKLIPLVSYEQ 74
           K  L  ++ +T+N  +IQ  +L  ILS NA+ EYL    L+      +FKK +P+V+YE 
Sbjct: 89  KACLSLLEDVTTNVKQIQDSVLEAILSRNAHTEYLSGF-LNGQADKKSFKKNVPVVTYED 148

Query: 75  LKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRLSFFNYLMART 134
           +KPYI RIA G+ S ++C  PI+    SSGTSGG PKL+P+  +E E+R+SF +      
Sbjct: 149 IKPYIDRIANGEPSDLICDRPISVLLTSSGTSGGVPKLIPLTTEELEQRISFASLYRPLL 208

Query: 135 KELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNLKSVESGNNAI-PDD 194
            +    I   + K+   +F   + +T +GILV T+ + +LK      S     + I P  
Sbjct: 209 YKYIEGIR--ERKSFMLYFVTRESETASGILVRTMITCVLKSVTPANSFIWDQSQISPHA 268

Query: 195 ILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVDLVSDIRRGSIN 254
           I  C++T QS+YCQLLCGL Q D V ++GA  AS  + + KFLE+HW +  S+IR G ++
Sbjct: 269 ITTCSDTTQSMYCQLLCGLLQRDNVGRLGAPFASSFLKIIKFLEDHWPEFCSNIRTGCLS 328

Query: 255 NPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTKYIKAIATGSLS 314
           +  ITD      + K L  PNP+LA LIE ECS+  W+ IV +LWP  K I+AI TG+++
Sbjct: 329 D-WITDPQCVSGIGKFLTAPNPELASLIEQECSQTSWEAIVKRLWPKAKCIEAIVTGTMA 388

Query: 315 QYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMAYFEFLPIDTTN 374
           QY PLL +Y+  LP+ S  YGS+ECF GLNL+P+  PNE SYT+IP MAYFEFL ++   
Sbjct: 389 QYNPLLEFYSGGLPVISTFYGSSECFFGLNLNPLSKPNEVSYTIIPCMAYFEFLEVEKDY 448

Query: 375 INGEVTQE---LVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTNKAPKISFVRR 434
            +G    E   +VDLVDVK+G +YE V+TTFAGLYR  LGD++RVTGF N AP+  FV R
Sbjct: 449 ESGHDPAENPVVVDLVDVKIGHDYEPVVTTFAGLYRYRLGDVLRVTGFYNNAPQFHFVGR 508

Query: 435 KNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGHYVLYWELLID 494
           + VVL+++ +KT + DL   V NA  +L+P    ++D+TS  D+S+ PGHYVLYWEL   
Sbjct: 509 QKVVLSIDMDKTYDEDLLKAVTNASLLLEPHDLMLMDFTSRVDSSSYPGHYVLYWELGRK 568

Query: 495 GNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIVKSGTFEKLMK 554
             D   +    +  +V  +CCF IEES    YR  R ++K I PLEI++VK G F+KLM 
Sbjct: 569 VKDAKLE----LDQNVLEECCFTIEESLDAVYRKGRKNDKNIGPLEIKVVKPGAFDKLMN 628

Query: 555 LAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNP 594
             ++ G+S++QYKTPRS+ + +   +++LE+NVV  + SQK P
Sbjct: 629 FFLSRGSSVSQYKTPRSVTNEEA--LKILEANVVSEFLSQKTP 661

BLAST of CSPI02G07750 vs. NCBI nr
Match: gi|449442409|ref|XP_004138974.1| (PREDICTED: indole-3-acetic acid-amido synthetase GH3.5-like isoform X1 [Cucumis sativus])

HSP 1 Score: 1216.1 bits (3145), Expect = 0.0e+00
Identity = 595/597 (99.66%), Postives = 595/597 (99.66%), Query Frame = 1

Query: 1   MPSHVEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS 60
           MPSHVEESWLDERDKE LQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS
Sbjct: 1   MPSHVEESWLDERDKEALQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS 60

Query: 61  STFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEF 120
           STFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEF
Sbjct: 61  STFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEF 120

Query: 121 ERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNL 180
           ERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNL
Sbjct: 121 ERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNL 180

Query: 181 KSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHW 240
           KSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHW
Sbjct: 181 KSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHW 240

Query: 241 VDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPN 300
           VDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPN
Sbjct: 241 VDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPN 300

Query: 301 TKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPT 360
           TKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPT
Sbjct: 301 TKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPT 360

Query: 361 MAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTN 420
           MAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTN
Sbjct: 361 MAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTN 420

Query: 421 KAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGH 480
           KAPK SFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGH
Sbjct: 421 KAPKFSFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGH 480

Query: 481 YVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIV 540
           YVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIV
Sbjct: 481 YVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIV 540

Query: 541 KSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL 598
           KSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL
Sbjct: 541 KSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL 597

BLAST of CSPI02G07750 vs. NCBI nr
Match: gi|778668015|ref|XP_011649024.1| (PREDICTED: indole-3-acetic acid-amido synthetase GH3.5-like isoform X2 [Cucumis sativus])

HSP 1 Score: 1177.9 bits (3046), Expect = 0.0e+00
Identity = 581/597 (97.32%), Postives = 581/597 (97.32%), Query Frame = 1

Query: 1   MPSHVEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS 60
           MPSHVEESWLDERDKE LQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS
Sbjct: 1   MPSHVEESWLDERDKEALQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGS 60

Query: 61  STFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEF 120
           STFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEF
Sbjct: 61  STFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEF 120

Query: 121 ERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNL 180
           ERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNL
Sbjct: 121 ERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNL 180

Query: 181 KSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHW 240
           KSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHW
Sbjct: 181 KSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHW 240

Query: 241 VDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPN 300
           VDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPN
Sbjct: 241 VDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPN 300

Query: 301 TKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPT 360
           TKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPT
Sbjct: 301 TKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPT 360

Query: 361 MAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTN 420
           MAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFA              GFTN
Sbjct: 361 MAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFA--------------GFTN 420

Query: 421 KAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGH 480
           KAPK SFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGH
Sbjct: 421 KAPKFSFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGH 480

Query: 481 YVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIV 540
           YVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIV
Sbjct: 481 YVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIV 540

Query: 541 KSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL 598
           KSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL
Sbjct: 541 KSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL 583

BLAST of CSPI02G07750 vs. NCBI nr
Match: gi|659082377|ref|XP_008441807.1| (PREDICTED: indole-3-acetic acid-amido synthetase GH3.5-like [Cucumis melo])

HSP 1 Score: 1086.6 bits (2809), Expect = 0.0e+00
Identity = 531/598 (88.80%), Postives = 561/598 (93.81%), Query Frame = 1

Query: 1   MPSHVEESWL-DERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTG 60
           M S+VE  WL DE+D E LQ IQ ITSNA EIQ  IL+EILSTNANVEYLQQHGL  ST 
Sbjct: 1   MSSNVEHKWLLDEKDYEALQQIQDITSNADEIQSLILTEILSTNANVEYLQQHGLDGSTD 60

Query: 61  SSTFKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKE 120
           SSTFKKLIPLVSYEQL+PYITRIAEGDDSPILCSNPIT FF+SSGTSGGEPKLVPIYE+E
Sbjct: 61  SSTFKKLIPLVSYEQLQPYITRIAEGDDSPILCSNPITGFFVSSGTSGGEPKLVPIYEQE 120

Query: 121 FERRLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLN 180
           FERRLSFFNYLMARTK+LFPNIN +  KAMNFHFAKPDHKTKAGILV +VF+RLLK SLN
Sbjct: 121 FERRLSFFNYLMARTKQLFPNINCYNDKAMNFHFAKPDHKTKAGILVSSVFTRLLKTSLN 180

Query: 181 LKSVESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENH 240
           LKS+ +GNNAIPDDIL CT+TYQSLYCQLLCGLY+NDLVFQVG++ ASGL+HVFKFLENH
Sbjct: 181 LKSIATGNNAIPDDILLCTDTYQSLYCQLLCGLYRNDLVFQVGSIFASGLLHVFKFLENH 240

Query: 241 WVDLVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWP 300
           W DLVSDIR GSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKG WKGIV +LWP
Sbjct: 241 WADLVSDIRTGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGTWKGIVRRLWP 300

Query: 301 NTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIP 360
           NTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTEC LGLNLDPICDPNETSYTLIP
Sbjct: 301 NTKYIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECLLGLNLDPICDPNETSYTLIP 360

Query: 361 TMAYFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFT 420
           TMAYFEFLP+DTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYR  +GDI+RVTGFT
Sbjct: 361 TMAYFEFLPMDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRYCIGDILRVTGFT 420

Query: 421 NKAPKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPG 480
           NKAPK SFVRRKNV+LNLEYEKTNE+DLR+GVE AGGVLKPFGATIVDYT YADTSTIPG
Sbjct: 421 NKAPKFSFVRRKNVILNLEYEKTNESDLRLGVEKAGGVLKPFGATIVDYTCYADTSTIPG 480

Query: 481 HYVLYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRI 540
           HYVLYWELLIDGNDHNTQTNHFIPSSV+NDCC AIE+S +TFYRIKRS EKTINPLEIR+
Sbjct: 481 HYVLYWELLIDGNDHNTQTNHFIPSSVYNDCCLAIEDSLSTFYRIKRSQEKTINPLEIRV 540

Query: 541 VKSGTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRVGL 598
           VKSGTFEKLM+LAINGGASMNQYKTPRSLNSNQIHFIQLLESNV+ SYFSQKNPRVGL
Sbjct: 541 VKSGTFEKLMQLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVISSYFSQKNPRVGL 598

BLAST of CSPI02G07750 vs. NCBI nr
Match: gi|659094768|ref|XP_008448232.1| (PREDICTED: indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo])

HSP 1 Score: 884.8 bits (2285), Expect = 8.5e-254
Identity = 427/593 (72.01%), Postives = 494/593 (83.31%), Query Frame = 1

Query: 3   SHVEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSST 62
           S+ EE WLDE+DKE LQ+I+ IT+ A EIQRQIL+EILS NANVEYLQ+HGL AST SST
Sbjct: 2   SNFEEKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSST 61

Query: 63  FKKLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFER 122
           FKKLIPLV Y+QL+PYI RIA+GDDS ILCSNPIT FF SSGTSG E KL+P+YE+EF R
Sbjct: 62  FKKLIPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFVR 121

Query: 123 RLSFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNLKS 182
           RLSFF+Y+  R K+LFPNINWHK K +NFHFAKP+ KTK GI+V ++F+ L KRS NL+S
Sbjct: 122 RLSFFSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLRS 181

Query: 183 VESGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVD 242
           + SGNN  PDDI+ CT++YQSLYCQLLCGLYQND+VF+V A+ AS LIHVFKFLENHW+D
Sbjct: 182 MPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWID 241

Query: 243 LVSDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTK 302
           L +DIR  +IN PKIT  S+RESVMKI+VKPNP++ADLIE EC KG+W+GI+ +LWPN K
Sbjct: 242 LATDIRTRTIN-PKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAK 301

Query: 303 YIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMA 362
           YI A+ TGS+SQYIPLLNYYTNNLPI SDHYGS+ECFLGLNLDP+C+P+E SYTLIPTMA
Sbjct: 302 YINAVVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTMA 361

Query: 363 YFEFLPIDTTNINGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTNKA 422
           YFEFLPID  N  GEV Q LVDLVDVKLG EYELVITTFAGLYR  +GD+VRVTGF NKA
Sbjct: 362 YFEFLPIDMLNPKGEVNQRLVDLVDVKLGHEYELVITTFAGLYRYCMGDVVRVTGFKNKA 421

Query: 423 PKISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGHYV 482
           P  SFVRRKNVVLN+  EKT+E  L   VE  G V+K FGATIVDYT+YAD STIPGHYV
Sbjct: 422 PNFSFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATIVDYTTYADISTIPGHYV 481

Query: 483 LYWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIVKS 542
           LYWEL +D  D   Q  ++IP SVF DCC AIE S    YR+ RSHEK INPLEI+IVK+
Sbjct: 482 LYWELTLD--DLKEQNLNYIPCSVFEDCCLAIENSLNLLYRLARSHEKCINPLEIKIVKA 541

Query: 543 GTFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRV 596
           GTFEKLM+LA+N GAS+ QYKTPR LNS+QIH IQLLESN+V +YFS+  P +
Sbjct: 542 GTFEKLMQLALNRGASITQYKTPRCLNSSQIHIIQLLESNLVSNYFSKNYPNL 591

BLAST of CSPI02G07750 vs. NCBI nr
Match: gi|449444564|ref|XP_004140044.1| (PREDICTED: indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis sativus])

HSP 1 Score: 874.0 bits (2257), Expect = 1.5e-250
Identity = 426/592 (71.96%), Postives = 495/592 (83.61%), Query Frame = 1

Query: 5   VEESWLDERDKEVLQHIQHITSNAGEIQRQILSEILSTNANVEYLQQHGLHASTGSSTFK 64
           VEE WLDE++KE LQ+I+ IT+ A EIQRQIL+EILSTNANVEYLQQHGL   T S TFK
Sbjct: 4   VEEKWLDEKEKEALQYIEDITTKADEIQRQILNEILSTNANVEYLQQHGLVVPTDSPTFK 63

Query: 65  KLIPLVSYEQLKPYITRIAEGDDSPILCSNPITAFFLSSGTSGGEPKLVPIYEKEFERRL 124
           KLIPLV YEQL+PYI RIA+GDDS ILCSNPIT FF SSGTSGGE KL+P+YE+EF RRL
Sbjct: 64  KLIPLVCYEQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGGEHKLIPMYEQEFVRRL 123

Query: 125 SFFNYLMARTKELFPNINWHKGKAMNFHFAKPDHKTKAGILVHTVFSRLLKRSLNLKSVE 184
           SFF+Y+M R K+LFP+INWHK K +NFHFAKP+ KTK GI+V ++F+ L KRS NL+S+ 
Sbjct: 124 SFFSYIMPRMKQLFPDINWHKVKGLNFHFAKPEFKTKGGIIVRSIFTNLHKRSSNLESMP 183

Query: 185 SGNNAIPDDILRCTNTYQSLYCQLLCGLYQNDLVFQVGAVLASGLIHVFKFLENHWVDLV 244
           SGNN  PDDI+ CT++YQSLYCQLLCGLYQN++VF+V A+ AS LIHVFKFLENHWVDL 
Sbjct: 184 SGNNTSPDDIILCTDSYQSLYCQLLCGLYQNEVVFRVSALFASTLIHVFKFLENHWVDLA 243

Query: 245 SDIRRGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGKWKGIVPKLWPNTKYI 304
           +DIR  ++ NPKIT+ S+RES+MKI+VKPNP++ADLIE EC KG+W+GI+ +LWPN KYI
Sbjct: 244 TDIRTRTV-NPKITNSSVRESLMKIIVKPNPEVADLIENECRKGRWEGIITRLWPNAKYI 303

Query: 305 KAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECFLGLNLDPICDPNETSYTLIPTMAYF 364
            AI TGS+SQYIPLLNYYTNNLPI SDHYGS+ECFLGLNLDP+C+ +E SYTLIPTMAYF
Sbjct: 304 NAIVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNHDEVSYTLIPTMAYF 363

Query: 365 EFLPIDTTN-INGEVTQELVDLVDVKLGQEYELVITTFAGLYRCSLGDIVRVTGFTNKAP 424
           EFLPID  N  NGEV Q+LVDLVDVKLG+EYELVITTFAGLYR  +GDIVRVTGF NKAP
Sbjct: 364 EFLPIDMINDPNGEVNQQLVDLVDVKLGREYELVITTFAGLYRYCMGDIVRVTGFKNKAP 423

Query: 425 KISFVRRKNVVLNLEYEKTNETDLRMGVENAGGVLKPFGATIVDYTSYADTSTIPGHYVL 484
              FVRRKNVVLN+  EKT+E  L   VE  G V+K FGA IVDYT+YAD STIPGHYVL
Sbjct: 424 SFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKIVDYTTYADLSTIPGHYVL 483

Query: 485 YWELLIDGNDHNTQTNHFIPSSVFNDCCFAIEESFTTFYRIKRSHEKTINPLEIRIVKSG 544
           YWEL +D  D   Q ++ IPSSVF DCC  IE S    YR+ RSHEK INPLEI+IVK+G
Sbjct: 484 YWELTMD--DLKEQNSNDIPSSVFEDCCLDIENSLNLLYRLARSHEKCINPLEIKIVKAG 543

Query: 545 TFEKLMKLAINGGASMNQYKTPRSLNSNQIHFIQLLESNVVCSYFSQKNPRV 596
           TFEKLM+LA++ GAS+ QYKTPR LNS+QIH IQLLESNVV +YFS+K P +
Sbjct: 544 TFEKLMQLALDRGASITQYKTPRCLNSSQIHIIQLLESNVVSNYFSRKYPNL 592

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
GH35_ARATH7.3e-17352.17Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana GN=GH3.5 PE=... [more]
GH36_ARATH5.8e-17051.08Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana GN=GH3.6 PE=... [more]
GH31_ORYSJ1.7e-15347.00Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo... [more]
GH34_ORYSJ8.7e-15046.10Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo... [more]
GH38_ORYSI1.1e-14746.32Probable indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indi... [more]
Match NameE-valueIdentityDescription
A0A0A0LHA0_CUCSA0.0e+0099.66Uncharacterized protein OS=Cucumis sativus GN=Csa_2G081190 PE=4 SV=1[more]
A0A0A0KAF9_CUCSA1.0e-25071.96Uncharacterized protein OS=Cucumis sativus GN=Csa_6G125240 PE=4 SV=1[more]
I1LRV0_SOYBN1.9e-17553.02Uncharacterized protein OS=Glycine max GN=GLYMA_12G103500 PE=4 SV=2[more]
V7AHM5_PHAVU5.5e-17552.22Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_011G153400g PE=4 SV=1[more]
A0A0S3S9C7_PHAAN9.3e-17552.39Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.06G035000 PE=... [more]
Match NameE-valueIdentityDescription
AT4G27260.14.1e-17452.17 Auxin-responsive GH3 family protein[more]
AT5G54510.13.3e-17151.08 Auxin-responsive GH3 family protein[more]
AT4G37390.16.2e-14644.02 Auxin-responsive GH3 family protein[more]
AT2G14960.11.2e-14445.30 Auxin-responsive GH3 family protein[more]
AT5G13360.39.9e-14445.11 Auxin-responsive GH3 family protein[more]
Match NameE-valueIdentityDescription
gi|449442409|ref|XP_004138974.1|0.0e+0099.66PREDICTED: indole-3-acetic acid-amido synthetase GH3.5-like isoform X1 [Cucumis ... [more]
gi|778668015|ref|XP_011649024.1|0.0e+0097.32PREDICTED: indole-3-acetic acid-amido synthetase GH3.5-like isoform X2 [Cucumis ... [more]
gi|659082377|ref|XP_008441807.1|0.0e+0088.80PREDICTED: indole-3-acetic acid-amido synthetase GH3.5-like [Cucumis melo][more]
gi|659094768|ref|XP_008448232.1|8.5e-25472.01PREDICTED: indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo][more]
gi|449444564|ref|XP_004140044.1|1.5e-25071.96PREDICTED: indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR004993GH3
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G07750.1CSPI02G07750.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPANTHERPTHR31901FAMILY NOT NAMEDcoord: 10..598
score: 5.0E
IPR004993GH3 familyPFAMPF03321GH3coord: 21..571
score: 1.3E