CSPI02G05810 (gene) Wild cucumber (PI 183967)

NameCSPI02G05810
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionGlucose-methanol-choline (GMC) oxidoreductase family protein
LocationChr2 : 4271346 .. 4278925 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCCAGCTTTGTAATCCTAAATATATATCTATATAAACCAACAGTTTGCAAATTAGAAAGGAACAAAGAAGTACCCCCACATATACATCTACATCTTTGAATAAGCATGGAGTATTCAATAGCAACCTTTCTCCCTCTCATCCTAATCTCCATTGAATTTCTCTCTTCACATGCCATCCCTAATCAAGGCAAAACCCTGATCTCTCTATATCTTTCTTTTTCTAAACTTGTATCGTGTTTAGTTTATTGATTCTAACTGTTGTGTATTTTGTTACCTTTCCTTTTCTAGATATTAGCTACATGAAATTTGTACACAATGCCAATGATCAACCAATACAAGAAAAATATGACTACATAGTAATAGGAGGAGGAACAACAGGCTGTCCATTAGCTACAACATTATCATCAAAATTCTCAGTCCTCCTTCTTGAAAGAGGTAGTGACCCCAACACATATCCTTTTGTGTTGAATGAAGAAACTCCATCCTACACTTTTATTCTAGACGATGATGGCCAAAATCCCATCCAACGCTTTGTCTCTGAGGATGGTGTGGAGAACATACGAGGGCGAGTTCTTGGCGGCGGAAGCATGGTCAACGCCGGGTTCTACTCGAGGGGACATCAAGAGTTTTTTGAAACTGCAGGTGTGGATTGGGACATGGAATTGGTTGAGAAGGCTTACGAATGGGTTGAAGAAACTTTGGTGTCTAGGCCAAATTTAACTGCATGGCAAGCTGCTTTTAGAAATACTTTGTTGGAAGGTGGTGTTGTCCCTGATAATGGGTTTGATTTAAGGCATCTTGTGGGTACTAAAATTGGAGGTTCAATCTTTGATAACAAAGGAAATAGACATGGAGCTGTAGAGCTTCTCAATAAGGCTAATCCTAAAAATCTTAAGGTTGTCATTCAAGCCACAGTTCAAAGAATCATCTTCAAAGGTTAGTGGCTAACAAGTTAAATTTGGTCCTTTTTTTTTTTGGTTAATGAATTTTTAGAAGATGGTTTCTAACTTTTAAAAAGATAAGGGTTTTATATATATATATTGATATTAGAGAAAAAAATAATGATATTTTGGTCCATTTTAAGGTGCACTTTCATCACCCAACTCACTCACTGATCATCATGAAAGTAAAATATAATTTTTTTTAAAATTAATCTTTTCAGTACTCAAATATAATTTTGAAGAATAATGTGGGTTGGATTCTTAAGTTTTTAAAACACACCTTATCCTGTTATTATATAAAACTAAAATAGAAAATAGTTCCATGCACCTTTTGAACATTTTTTTTAAATTTCAAATTTAAAAACATATATTTTTAAAACTCAAGGAAAAATAGACTCGTTTTTTTTTTTTTTTTTTAAGAATTAAAAGATCAAAACTCACTGGTTCATAGACTAATACAGTACTAGTTTGCAGAATAAAAAATAAATATTTTTTAAGAAAATGGTGATGATCCAGTCTGTGTGTCTCCAAAACATAATCACATCAATGACATGTTAATAAATGCATGACAACGATATACCAATGGTTAAAACTTAAAAGCAAAACGAAATTAAACTTATCATCTCTACCTAAATAAATAATAAATACTTATGTTAACTACTACAAAATCTACAACTTACAATTTTTGAAACGTTGACACTTTTATGGGAAAACAAATGTCAAGTAATGTACTTAGAAACGTAAAATAGATAACATAATAAGCAAAAATTTCACAGAAGTTTTCGATCTTATTTTTTTCACGTTTTTCTACTTCCAACTGACAGGTTTATCAGCAGTTGGAGTTTTATATTCAGATTCAAAAGGAAAGTTACACACGACATTAATTCATAAAAAAGGAGAAATATTTTTAAGTGCAGGAGCTATTGGAAGTCCTCAACTTCTCCTTTTAAGTGGGATTGGCCCAAAATCTTATCTTTCATCTTTAAAACTACATCTCGTTCACCACCAACCACATGTGGGTCAATACATGACCGACAATCCCCGTTTTAGTCGTTCTATTATTTTTCCATTCCAGTTACTTGCTTCAACAGCGCAAGTTGTTGGAACATTAGAAAAAAACATCCATTTACAATCATTGGCCAGCCCTTTACCATTTTTCCCTTTACCATCTTATGGTCTTCTTCCTCCTCAATCCACTTCCATCACTTCAAGCTTAGTTATTTTTGTAGGTAAATTTTCTAATGTTAGTTCTAAAGGGTGGCTTCATTTGAATAATTCTTCCACTGATGCGAAGGAGAATCCTATTGTTAGATTCAATTATTATTCACAGCATGGTGATCTTAGTAGATGTGTTAGTGGAATGAGGAAAGTGGAGGATTTGCTTAAGACACAAACTATGGAAAGGATTAAGACACAAGATTTGGAGGGTAATAAAGGATTTCAATTTATGGAACTTCCAATGCCTGAAAATTTGTGGAATGATAGCTCTGTTGAAGAGTATTGCAAAAATACGGTGGCTACTTATTGGCATTATCATGGAGGATGTTTGGTTGGAAAAGTTGTGGATGATAATTATAAAGTGATTGGAATTAAAAATTTGCGTGTTGTTGATGGCTCCACTTTCTCCGACTCGCCCGGAACTAATCCTATGGCCACCCTTATGATGTTAGGCCGGTGAGTTTATTTATTTATTTATCTTTCTACCTGGTGGATCTCTCAAAATATATATATGTGTGTATGTGTGTGTGGTTTGATTAAGTTTGGTTTGGTTTTTGGTTTAATATATAATATAAACAGTTTGTGTTTTGAACCTTGATACCTGTTTATAAGAAGCATTTTTGTAGTAAAAAAAAAAATGCTACTTCTTGTTATTATTTGAAATAAATGAAGGGCTTGGACCTTTGCACTATTAGTTGAACTTAGTTTTTTCTCTCTTTTGCATTATCTTTTATTTCCATAATTAAACTTACAAGTTCTAACTTGTTTTGCAGATATGTTGGCCTTAAAGTACTGCAACAAAGATCAAGTTAAAAGTGTAAATCAAGATAAATGATGAGAGATTTAAATCAGGGACTTTTAATTTAATTTCCTTACTAACATATTGTGCATGCATCTTATTTTTATCATTTTTCACTACTATATGTCTTTGTTTAGATGATATAAGGTTCAATTTACTTTTACCGATATGCTTAAATGAATGGGTCCAATCATTTATCATTGCTATTGGAGAATATGCTCCTGAATCCTTGCCTGTTTCAGTCAATAACCATTTAAGATGTTTAATAAATGTTCTTTTTCGTTTACA

mRNA sequence

ATGGAGTATTCAATAGCAACCTTTCTCCCTCTCATCCTAATCTCCATTGAATTTCTCTCTTCACATGCCATCCCTAATCAAGATATTAGCTACATGAAATTTGTACACAATGCCAATGATCAACCAATACAAGAAAAATATGACTACATAGTAATAGGAGGAGGAACAACAGGCTGTCCATTAGCTACAACATTATCATCAAAATTCTCAGTCCTCCTTCTTGAAAGAGGTAGTGACCCCAACACATATCCTTTTGTGTTGAATGAAGAAACTCCATCCTACACTTTTATTCTAGACGATGATGGCCAAAATCCCATCCAACGCTTTGTCTCTGAGGATGGTGTGGAGAACATACGAGGGCGAGTTCTTGGCGGCGGAAGCATGGTCAACGCCGGGTTCTACTCGAGGGGACATCAAGAGTTTTTTGAAACTGCAGGTGTGGATTGGGACATGGAATTGGTTGAGAAGGCTTACGAATGGGTTGAAGAAACTTTGGTGTCTAGGCCAAATTTAACTGCATGGCAAGCTGCTTTTAGAAATACTTTGTTGGAAGGTGGTGTTGTCCCTGATAATGGGTTTGATTTAAGGCATCTTGTGGGTACTAAAATTGGAGGTTCAATCTTTGATAACAAAGGAAATAGACATGGAGCTGTAGAGCTTCTCAATAAGGCTAATCCTAAAAATCTTAAGGTTGTCATTCAAGCCACAGTTCAAAGAATCATCTTCAAAGGTTTATCAGCAGTTGGAGTTTTATATTCAGATTCAAAAGGAAAGTTACACACGACATTAATTCATAAAAAAGGAGAAATATTTTTAAGTGCAGGAGCTATTGGAAGTCCTCAACTTCTCCTTTTAAGTGGGATTGGCCCAAAATCTTATCTTTCATCTTTAAAACTACATCTCGTTCACCACCAACCACATGTGGGTCAATACATGACCGACAATCCCCGTTTTAGTCGTTCTATTATTTTTCCATTCCAGTTACTTGCTTCAACAGCGCAAGTTGTTGGAACATTAGAAAAAAACATCCATTTACAATCATTGGCCAGCCCTTTACCATTTTTCCCTTTACCATCTTATGGTCTTCTTCCTCCTCAATCCACTTCCATCACTTCAAGCTTAGTTATTTTTGTAGGTAAATTTTCTAATGTTAGTTCTAAAGGGTGGCTTCATTTGAATAATTCTTCCACTGATGCGAAGGAGAATCCTATTGTTAGATTCAATTATTATTCACAGCATGGTGATCTTAGTAGATGTGTTAGTGGAATGAGGAAAGTGGAGGATTTGCTTAAGACACAAACTATGGAAAGGATTAAGACACAAGATTTGGAGGGTAATAAAGGATTTCAATTTATGGAACTTCCAATGCCTGAAAATTTGTGGAATGATAGCTCTGTTGAAGAGTATTGCAAAAATACGGTGGCTACTTATTGGCATTATCATGGAGGATGTTTGGTTGGAAAAGTTGTGGATGATAATTATAAAGTGATTGGAATTAAAAATTTGCGTGTTGTTGATGGCTCCACTTTCTCCGACTCGCCCGGAACTAATCCTATGGCCACCCTTATGATGTTAGGCCGATATGTTGGCCTTAAAGTACTGCAACAAAGATCAAGTTAA

Coding sequence (CDS)

ATGGAGTATTCAATAGCAACCTTTCTCCCTCTCATCCTAATCTCCATTGAATTTCTCTCTTCACATGCCATCCCTAATCAAGATATTAGCTACATGAAATTTGTACACAATGCCAATGATCAACCAATACAAGAAAAATATGACTACATAGTAATAGGAGGAGGAACAACAGGCTGTCCATTAGCTACAACATTATCATCAAAATTCTCAGTCCTCCTTCTTGAAAGAGGTAGTGACCCCAACACATATCCTTTTGTGTTGAATGAAGAAACTCCATCCTACACTTTTATTCTAGACGATGATGGCCAAAATCCCATCCAACGCTTTGTCTCTGAGGATGGTGTGGAGAACATACGAGGGCGAGTTCTTGGCGGCGGAAGCATGGTCAACGCCGGGTTCTACTCGAGGGGACATCAAGAGTTTTTTGAAACTGCAGGTGTGGATTGGGACATGGAATTGGTTGAGAAGGCTTACGAATGGGTTGAAGAAACTTTGGTGTCTAGGCCAAATTTAACTGCATGGCAAGCTGCTTTTAGAAATACTTTGTTGGAAGGTGGTGTTGTCCCTGATAATGGGTTTGATTTAAGGCATCTTGTGGGTACTAAAATTGGAGGTTCAATCTTTGATAACAAAGGAAATAGACATGGAGCTGTAGAGCTTCTCAATAAGGCTAATCCTAAAAATCTTAAGGTTGTCATTCAAGCCACAGTTCAAAGAATCATCTTCAAAGGTTTATCAGCAGTTGGAGTTTTATATTCAGATTCAAAAGGAAAGTTACACACGACATTAATTCATAAAAAAGGAGAAATATTTTTAAGTGCAGGAGCTATTGGAAGTCCTCAACTTCTCCTTTTAAGTGGGATTGGCCCAAAATCTTATCTTTCATCTTTAAAACTACATCTCGTTCACCACCAACCACATGTGGGTCAATACATGACCGACAATCCCCGTTTTAGTCGTTCTATTATTTTTCCATTCCAGTTACTTGCTTCAACAGCGCAAGTTGTTGGAACATTAGAAAAAAACATCCATTTACAATCATTGGCCAGCCCTTTACCATTTTTCCCTTTACCATCTTATGGTCTTCTTCCTCCTCAATCCACTTCCATCACTTCAAGCTTAGTTATTTTTGTAGGTAAATTTTCTAATGTTAGTTCTAAAGGGTGGCTTCATTTGAATAATTCTTCCACTGATGCGAAGGAGAATCCTATTGTTAGATTCAATTATTATTCACAGCATGGTGATCTTAGTAGATGTGTTAGTGGAATGAGGAAAGTGGAGGATTTGCTTAAGACACAAACTATGGAAAGGATTAAGACACAAGATTTGGAGGGTAATAAAGGATTTCAATTTATGGAACTTCCAATGCCTGAAAATTTGTGGAATGATAGCTCTGTTGAAGAGTATTGCAAAAATACGGTGGCTACTTATTGGCATTATCATGGAGGATGTTTGGTTGGAAAAGTTGTGGATGATAATTATAAAGTGATTGGAATTAAAAATTTGCGTGTTGTTGATGGCTCCACTTTCTCCGACTCGCCCGGAACTAATCCTATGGCCACCCTTATGATGTTAGGCCGATATGTTGGCCTTAAAGTACTGCAACAAAGATCAAGTTAA
BLAST of CSPI02G05810 vs. Swiss-Prot
Match: MDL3_PRUSE ((R)-mandelonitrile lyase 3 OS=Prunus serotina GN=MDL3 PE=2 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 7.9e-143
Identity = 253/537 (47.11%), Postives = 354/537 (65.92%), Query Frame = 1

Query: 4   SIATFLPLILISIEFLSSHAIPN---QDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP 63
           ++   L + ++ +++    ++ N    D SY+ FV++A D  ++  YDYI++GGGT GCP
Sbjct: 8   AVLLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGGGTAGCP 67

Query: 64  LATTLSSKFSVLLLERGSDPNTYPFVLNEETPSYTFILDDDGQNPIQRFVSEDGVENIRG 123
           LA TLS+ +SVL+LERGS P  YP +L  +   Y    +DDG+ P++RFVSEDG++N+RG
Sbjct: 68  LAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDGIDNVRG 127

Query: 124 RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN 183
           RVLGG SM+NAG Y R +  FF   G++WDM+LV + YEWVE+T+V  P+   WQ     
Sbjct: 128 RVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQTWQTVIGT 187

Query: 184 TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI 243
             LE G++P+NGF + HL GT++ GS FDN G RH + ELLNK +P NL+V +QA V++I
Sbjct: 188 AYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQAAVEKI 247

Query: 244 IF----KGLSAVGVLYSDSKGKLHTTLIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSS 303
           IF     G++A+GV+Y+DS G  H   +  +GE+ LSAG IGSPQLLLLSG+GP+SYL+S
Sbjct: 248 IFSSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPESYLTS 307

Query: 304 LKLHLVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLAS------ 363
           L + +V   P+VGQY+ DNPR   +I+ P  + AST  V+G +  + +  S++S      
Sbjct: 308 LNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLG-ITSDFYQCSISSLPFDTP 367

Query: 364 PLPFFPLPSYGLLPPQSTSITSSLVIFVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYY 423
           P  FFP  SY L  P  T         V K     S G + L NSS+D +  P V+FNYY
Sbjct: 368 PFSFFPTTSYPL--PNQT-----FAHIVNKVPGPLSHGTVTL-NSSSDVRVGPNVKFNYY 427

Query: 424 SQHGDLSRCVSGMRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYC 483
           S   DLS CVSGM+K+ ++L T  +E  K +DL G  GF  + +P+PEN  +D++ E +C
Sbjct: 428 SNLTDLSHCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFC 487

Query: 484 KNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 528
           + +VA+YWHYHGGCLVGKV+DD ++V GI  LRVVDGSTF  +P ++P    +MLGR
Sbjct: 488 RESVASYWHYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGR 535

BLAST of CSPI02G05810 vs. Swiss-Prot
Match: MDL1_PRUSE ((R)-mandelonitrile lyase 1 OS=Prunus serotina GN=MDL1 PE=1 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 1.4e-139
Identity = 254/547 (46.44%), Postives = 357/547 (65.27%), Query Frame = 1

Query: 4   SIATFLPLILISIEFLSSHAIP---NQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP 63
           +I   L L ++ +++   H++    N D SY++F ++A D  ++  YDY+++GGGT+GCP
Sbjct: 8   AILLVLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVGGGTSGCP 67

Query: 64  LATTLSSKFSVLLLERGSDPNTYPFVLNEETPSYTFILDDDGQNPIQRFVSEDGVENIRG 123
           LA TLS K+ VL+LERGS P  YP VL  +   Y    +DDG+ P++RFVSEDG++N+RG
Sbjct: 68  LAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVRG 127

Query: 124 RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN 183
           RVLGG SM+NAG Y+R +   +  +GVDWDM+LV K YEWVE+T+V +PN   WQ+    
Sbjct: 128 RVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQPWQSVTGT 187

Query: 184 TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI 243
             LE GV P++GF L H  GT+I GS FDNKG RH A ELLNK N  NL+V + A+V++I
Sbjct: 188 AFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKI 247

Query: 244 IFK---GLSAVGVLYSDSKGKLHTTLIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSL 303
           IF    GL+A GV+Y DS G  H   +  KGE+ +SAG IG+PQLLLLSG+GP+SYLSSL
Sbjct: 248 IFSNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSL 307

Query: 304 KLHLVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPL 363
            + +V   P+VGQ++ DNPR   +I+ P  +  +   V+G    N   Q   S LP F  
Sbjct: 308 NIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI--SNDFYQCSFSSLP-FTT 367

Query: 364 PSYGLLPPQSTSI-TSSLVIFVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDL 423
           P +   P  S  +  S+   F  K +   S G L L  SS++ + +P V+FNYYS   DL
Sbjct: 368 PPFSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTL-KSSSNVRVSPNVKFNYYSNPTDL 427

Query: 424 SRCVSGMRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVAT 483
           S CVSGM+K+ +LL T  ++  K +DL G +GF  + +P+P++  +D++ E +C+ +VA+
Sbjct: 428 SHCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCRESVAS 487

Query: 484 YWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRERMRRIGEE 543
           YWHYHGGCLVGKV+D +++V GI  LRVVDGSTF  +P ++P    +MLGR    +I +E
Sbjct: 488 YWHYHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQE 547

BLAST of CSPI02G05810 vs. Swiss-Prot
Match: MDL1_PRUDU ((R)-mandelonitrile lyase 1 OS=Prunus dulcis GN=MDL1 PE=1 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 4.1e-139
Identity = 247/527 (46.87%), Postives = 351/527 (66.60%), Query Frame = 1

Query: 9   LPLILISIEFLSSHAIPN---QDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTL 68
           L L+++ +++   H++ N    D SY+KFV+NA D  ++  YDYIVIGGGT+GCPLA TL
Sbjct: 13  LHLLVLHLQYSEVHSLANTSAHDFSYLKFVYNATDTSLEGSYDYIVIGGGTSGCPLAATL 72

Query: 69  SSKFSVLLLERGSDPNTYPFVLNEETPSYTFILDDDGQNPIQRFVSEDGVENIRGRVLGG 128
           S K+ VLLLERG+    YP  L  +  +Y     DDG+ P++RFVSEDG++N+R R+LGG
Sbjct: 73  SEKYKVLLLERGTIATEYPNTLTADGFAYNLQQQDDGKTPVERFVSEDGIDNVRARILGG 132

Query: 129 GSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRNTLLEG 188
            +++NAG Y+R +  F+   G++WD++LV K YEWVE+ +V +PN  +WQ+      LE 
Sbjct: 133 TTIINAGVYARANISFYSQTGIEWDLDLVNKTYEWVEDAIVVKPNNQSWQSVIGEGFLEA 192

Query: 189 GVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIF--- 248
           G++PDNGF L H  GT++ GS FDN G RH A ELLNK +P NL V +QA+V++I+F   
Sbjct: 193 GILPDNGFSLDHEAGTRLTGSTFDNNGTRHAADELLNKGDPNNLLVAVQASVEKILFSSN 252

Query: 249 -KGLSAVGVLYSDSKGKLHTTLIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHL 308
              LSA+GV+Y+DS G  H   +   GE+ +SAG IG+PQLLLLSG+GP+SYLSSL + +
Sbjct: 253 TSNLSAIGVIYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNITV 312

Query: 309 VHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSYG 368
           V   P+VGQ++ +NPR   +   P  + AS   V+G   ++ + Q   S LP F  P + 
Sbjct: 313 VQPNPYVGQFLYNNPRNFINNFPPNPIEASVVTVLGI--RSDYYQVSLSSLP-FSTPPFS 372

Query: 369 LLPPQSTSI-TSSLVIFVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCV 428
           L P  S  +  S+    V +     S G + L NSS+D +  P ++FNYYS   DL+ CV
Sbjct: 373 LFPTTSYPLPNSTFAHIVSQVPGPLSHGSVTL-NSSSDVRIAPNIKFNYYSNSTDLANCV 432

Query: 429 SGMRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVATYWHY 488
           SGM+K+ DLL+T+ +E  K +D+ G  GF ++ +P+PEN  +D+S E +C + VA+YWHY
Sbjct: 433 SGMKKLGDLLRTKALEPYKARDVLGIDGFNYLGVPLPENQTDDASFETFCLDNVASYWHY 492

Query: 489 HGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 528
           HGG LVGKV+DD+++V+GIK LRVVD STF   P ++P    +MLGR
Sbjct: 493 HGGSLVGKVLDDSFRVMGIKALRVVDASTFPYEPNSHPQGFYLMLGR 535

BLAST of CSPI02G05810 vs. Swiss-Prot
Match: MDL2_PRUSE ((R)-mandelonitrile lyase 2 OS=Prunus serotina GN=MDL2 PE=2 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 9.1e-139
Identity = 250/541 (46.21%), Postives = 355/541 (65.62%), Query Frame = 1

Query: 9   LPLILISIEFLSSHAIPN---QDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTL 68
           L L ++ +++    ++ N    D SY++FV++AND  ++  YDYI++GGGT GCPLA TL
Sbjct: 14  LHLFVLHLQYSEVQSLANTSAHDFSYLEFVYDANDTELEGTYDYIIVGGGTAGCPLAATL 73

Query: 69  SSKFSVLLLERGSDPNTYPFVLNEETPSYTFILDDDGQNPIQRFVSEDGVENIRGRVLGG 128
           S+ +SVL+LERG+ P  YP +L  +   Y    +DDGQ P++RFVS DG++N+RGRVLGG
Sbjct: 74  SANYSVLVLERGTLPTEYPNLLTSDGFIYNLQQEDDGQTPVERFVSGDGIDNVRGRVLGG 133

Query: 129 GSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRNTLLEG 188
            SM+NAG Y R +  FF   G++WDM+LV K Y+WVE+T+V +P+   WQ       LE 
Sbjct: 134 TSMINAGVYVRANTSFFNQTGIEWDMDLVNKTYDWVEDTIVFKPDFQFWQNLTGTAFLEV 193

Query: 189 GVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIF--- 248
           G++PDNGF L HL GT++ GS FDN G RH + ELLNK +P NL+V + A V++IIF   
Sbjct: 194 GILPDNGFSLDHLEGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVHAAVEKIIFSSD 253

Query: 249 -KGLSAVGVLYSDSKGKLHTTLIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHL 308
             G++A+GV+Y+DS G  H   +   GE+ LSAG IGSPQLLLLSG+G +SYL+SL + +
Sbjct: 254 SSGVTAIGVIYTDSNGTTHQAFVRGDGEVILSAGPIGSPQLLLLSGVGLESYLTSLNISV 313

Query: 309 VHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLAS------PLPFF 368
           V   P+VGQY+ DNPR   +I+ P  + AST  V+G +  + +  S++S      P  FF
Sbjct: 314 VASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLG-ITSDFYQCSISSLPFSTAPFGFF 373

Query: 369 PLPSYGLLPPQSTSITSSLVIFVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGD 428
           P P+Y L  P +T         V K     S G + L  S++D +  P V FNYYS   D
Sbjct: 374 PNPTYPL--PNTT-----FAHIVNKVPGPLSHGTV-LLQSTSDVRVAPNVTFNYYSNTTD 433

Query: 429 LSRCVSGMRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVA 488
           L+ CVSGM+K+ + L +  ++  K +DL G +GF  + +P+PEN  +D++ E +C+  VA
Sbjct: 434 LAHCVSGMKKIGEFLSSDALKPYKVEDLPGIEGFDILGIPLPENQTDDAAFETFCREAVA 493

Query: 489 TYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRERMRRIGE 537
           +YWHYHGGCLVG+V+DD+++V GI  LRVVDGSTF  +P ++P    +MLGR    +I +
Sbjct: 494 SYWHYHGGCLVGEVLDDDFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGTKILQ 545

BLAST of CSPI02G05810 vs. Swiss-Prot
Match: MDL2_PRUDU ((R)-mandelonitrile lyase 2 OS=Prunus dulcis GN=MDL2 PE=1 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 1.2e-138
Identity = 251/547 (45.89%), Postives = 359/547 (65.63%), Query Frame = 1

Query: 4   SIATFLPLILISIEFLSSHAIP---NQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP 63
           +I   L + ++ +++   H++    + D SY+ F ++A D  ++  YDY+++GGGT+GCP
Sbjct: 8   AILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVGGGTSGCP 67

Query: 64  LATTLSSKFSVLLLERGSDPNTYPFVLNEETPSYTFILDDDGQNPIQRFVSEDGVENIRG 123
           LA TLS K+ VL+LERGS P  YP VL  +   Y    +DDG+ P++RFVSEDG++N+RG
Sbjct: 68  LAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVRG 127

Query: 124 RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN 183
           RVLGG S++NAG Y+R +   +  +GVDWDM+LV + YEWVE+T+V +PN  +WQ+  + 
Sbjct: 128 RVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQSWQSVTKT 187

Query: 184 TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI 243
             LE GV P++GF L H  GT+I GS FDNKG RH A ELLNK N  NL+V + A+V++I
Sbjct: 188 AFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKI 247

Query: 244 IFK---GLSAVGVLYSDSKGKLHTTLIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSL 303
           IF    GL+A GV+Y DS G  H   +  KGE+ +SAG IG+PQLLLLSG+GP+SYLSSL
Sbjct: 248 IFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSL 307

Query: 304 KLHLVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPL 363
            + +V   P+VGQ++ DNPR   +I+ P  +  +   V+G    N   Q   S LP F  
Sbjct: 308 NIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI--SNDFYQCSFSSLP-FTT 367

Query: 364 PSYGLLPPQSTSI-TSSLVIFVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDL 423
           P +G  P  S  +  S+   F  K +   S G L L  SS++ + +P V+FNYYS   DL
Sbjct: 368 PPFGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTL-KSSSNVRVSPNVKFNYYSNLTDL 427

Query: 424 SRCVSGMRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVAT 483
           S CVSGM+K+ +LL T  ++  K +DL G +GF  + +P+P++  +D++ E +C+ +VA+
Sbjct: 428 SHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFCRESVAS 487

Query: 484 YWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGRERMRRIGEE 543
           YWHYHGGCLVGKV+D +++V GI  LRVVDGSTF  +P ++P    +MLGR    +I +E
Sbjct: 488 YWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQE 547

BLAST of CSPI02G05810 vs. TrEMBL
Match: A0A0A0LMB8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G055040 PE=3 SV=1)

HSP 1 Score: 1056.6 bits (2731), Expect = 1.1e-305
Identity = 522/527 (99.05%), Postives = 524/527 (99.43%), Query Frame = 1

Query: 1   MEYSIATFLPLILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP 60
           MEYSIATFLPLILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP
Sbjct: 1   MEYSIATFLPLILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP 60

Query: 61  LATTLSSKFSVLLLERGSDPNTYPFVLNEETPSYTFILDDDGQNPIQRFVSEDGVENIRG 120
           LATTLSSKFSVLLLERGSDPNTYPFVLNEET SYTFILDDDGQNPIQRFVSEDGVENIRG
Sbjct: 61  LATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRG 120

Query: 121 RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN 180
           RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN
Sbjct: 121 RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN 180

Query: 181 TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI 240
           TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI
Sbjct: 181 TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI 240

Query: 241 IFKGLSAVGVLYSDSKGKLHTTLIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLH 300
           IFKGLSAVGVLYSDSKGKLHT LIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLH
Sbjct: 241 IFKGLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLH 300

Query: 301 LVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSY 360
           LVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSY
Sbjct: 301 LVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSY 360

Query: 361 GLLPPQSTSITSSLVIFVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCV 420
           GLLPPQSTSITSSLVI VGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGD+SRCV
Sbjct: 361 GLLPPQSTSITSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDISRCV 420

Query: 421 SGMRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVATYWHY 480
           SG+RKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVATYWHY
Sbjct: 421 SGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVATYWHY 480

Query: 481 HGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 528
           HGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR
Sbjct: 481 HGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 527

BLAST of CSPI02G05810 vs. TrEMBL
Match: A0A0A0LK76_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G055000 PE=3 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 1.1e-228
Identity = 398/531 (74.95%), Postives = 453/531 (85.31%), Query Frame = 1

Query: 1   MEYSIATFLPLILI----SIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGT 60
           ME+S  T L L L+     ++  SSHAIPNQD+SYMKFVHNA D P +E+YDYI+IGGGT
Sbjct: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT 60

Query: 61  TGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEETPSYTFILDDDGQNPIQRFVSEDGVE 120
            GCPLATTLSSKFSVLLLERGSDPN YP VL+E+     F   DDG+NP QRFVSEDGVE
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQA 180
           NIRGRVLGGGSMVNAGFYSRGH+EFF +AGVDWDMELVEKAYEWVEET+VS+P L AWQ+
Sbjct: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQAT 240
           AFR++LLEGGVVPD+GFDLRHLVGTK GGSIFDNKGNRHGAVELLNKANP N+KV I+AT
Sbjct: 181 AFRSSLLEGGVVPDSGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240

Query: 241 VQRIIFKGLSAVGVLYSDSKGKLHTTLIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSS 300
           VQRI+F GLSA GVLYSDSKGKLH  +I KKGEI +SAGAIGSPQLLLLSGIGPKS+LSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS 300

Query: 301 LKLHLVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFP 360
           LKL +V HQP+VGQ M+DNPRF  +II PF +L S+ +VVG L+ NI++QS+ASP P   
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI 360

Query: 361 LPSYGLLPPQSTSITSSLVIFVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDL 420
              + LLPPQ+TSI  +L +FVGKFS V S+G L L NSST+ K++PIV FNYYS   DL
Sbjct: 361 PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRL-NSSTNVKKSPIVGFNYYSHPDDL 420

Query: 421 SRCVSGMRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVAT 480
            RCV G+RK+ DLLKT+TME+IKT++LEGNKGF+F+ +P+PENLWNDSSVEEYCK TV T
Sbjct: 421 GRCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTT 480

Query: 481 YWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 528
           YWHYHGGCLVGKVVD NYKVIGIKNLRVVDGSTFS+SPGTNPMATLMMLGR
Sbjct: 481 YWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 530

BLAST of CSPI02G05810 vs. TrEMBL
Match: A0A0A0LK79_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G055050 PE=3 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 7.3e-228
Identity = 395/527 (74.95%), Postives = 444/527 (84.25%), Query Frame = 1

Query: 1   MEYSIATFLPLILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP 60
           M   I  F  + +  +  ++SH IPNQD SYMKFV+ A++    E+YDYI+IGGGT GCP
Sbjct: 5   MANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGTAGCP 64

Query: 61  LATTLSSKFSVLLLERGSDPNTYPFVLNEETPSYTFILDDDGQNPIQRFVSEDGVENIRG 120
           LA TLSSKFSVLLLERG+DPN YP VLNE+     F+ +DDGQNP Q F+SEDGVEN+RG
Sbjct: 65  LAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRG 124

Query: 121 RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN 180
           RVLGGGSM+NAGFYSRGH++FFETAGVDWD ELVEKAYEWVEET+VS+P+L+ WQAAFR+
Sbjct: 125 RVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQAAFRS 184

Query: 181 TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI 240
            LLEGGV  D GFDLRHLVGTK GGSIFDNKGNRHGAVELLNK  PKNLKV  QATVQRI
Sbjct: 185 ALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQATVQRI 244

Query: 241 IFKGLSAVGVLYSDSKGKLHTTLIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLH 300
           IF GLSA GV YSDSKGKLHT  I KKGEI LSAGAIGSPQLLLLSG+GPKSYLSSL+L 
Sbjct: 245 IFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSSLRLP 304

Query: 301 LVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSY 360
           +V  QPHVG++M+DNPRFS +I+ PFQL++S+AQVVGTL+ NIHLQS ASPLPFF  PS+
Sbjct: 305 VVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFAPPSF 364

Query: 361 GLLPPQSTSITSSLVIFVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCV 420
            LLPPQ TSI  SL IFVGKFS+V S+G L L NSS D KE+PIVRFNYYS   DL+RCV
Sbjct: 365 SLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRL-NSSIDVKESPIVRFNYYSHPDDLARCV 424

Query: 421 SGMRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVATYWHY 480
            G+RKV DLLKT TME+IKTQDLEGNK FQF+ L +PENL ND++VEEYC+ TV TYWHY
Sbjct: 425 RGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTYWHY 484

Query: 481 HGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 528
           HGGCLVGKVVDDN+KVIGI NLRVVDGSTFS SPGTNPMATLMMLGR
Sbjct: 485 HGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGR 530

BLAST of CSPI02G05810 vs. TrEMBL
Match: A0A0A0LZU8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G627390 PE=3 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 2.0e-222
Identity = 389/529 (73.53%), Postives = 442/529 (83.55%), Query Frame = 1

Query: 3   YSIATFLPLILI----SIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTG 62
           YS A  L L LI     ++  SSHAIPNQD+SYMKFVHNA D P +E++DYI+IGGGT G
Sbjct: 4   YSKANILILNLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPRKEEFDYIIIGGGTAG 63

Query: 63  CPLATTLSSKFSVLLLERGSDPNTYPFVLNEETPSYTFILDDDGQNPIQRFVSEDGVENI 122
           CPLATTLSSKFSVLLLERGSDPN YP VLNE+     F  +DDG+ P Q FVSEDGVENI
Sbjct: 64  CPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGVENI 123

Query: 123 RGRVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAF 182
           RGRVLGGGSMVNAGFYSR H+EFFE+AGVDWDMELVEKAYEWVEE++VS+P L  WQ+AF
Sbjct: 124 RGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQSAF 183

Query: 183 RNTLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQ 242
           R+ LLEGG+VPDNGFDLRHLVGTK GGSI D+KGNRHGAVELLNKA+PKNLKV I+ATV+
Sbjct: 184 RSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEATVR 243

Query: 243 RIIFKGLSAVGVLYSDSKGKLHTTLIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLK 302
           RI+F  LSA GV Y DSKGKLHT  IH+KGEIFLSAGAIGSPQLLLLSGIGPKS+LSSLK
Sbjct: 244 RILFSDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSHLSSLK 303

Query: 303 LHLVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLP 362
           L +V HQPHVGQ MTDNPRF  +II PFQ++ ++ +VVG L+ NI+ QS+ASP PF   P
Sbjct: 304 LPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSPFLVPP 363

Query: 363 SYGLLPPQSTSITSSLVIFVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSR 422
           ++ LLPP  TSI  +L IF GKFS V SKG L L NSS D K++PIV+FNYYS   DL+R
Sbjct: 364 TFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKL-NSSIDVKKSPIVQFNYYSHPDDLAR 423

Query: 423 CVSGMRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVATYW 482
           CV G+RK+ D+ KT T+E+IKTQ+L+GNKGF F+ LP+P NLWNDS+VEEYCK TVATYW
Sbjct: 424 CVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYW 483

Query: 483 HYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 528
           HYHGGCLVGKVVD NYKVI  +NLRVVDGSTFS SPGTNPMATLMMLGR
Sbjct: 484 HYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGR 531

BLAST of CSPI02G05810 vs. TrEMBL
Match: A0A0A0LMB3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G050000 PE=3 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 9.9e-201
Identity = 344/503 (68.39%), Postives = 414/503 (82.31%), Query Frame = 1

Query: 26  NQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPF 85
           +QD+SYMKFVHNAN  P +E+YDYI+IGGGT GCPLA TLSSKFSVLLLERG+ PN +P 
Sbjct: 31  DQDVSYMKFVHNANKFPEKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGNIPNKFPS 90

Query: 86  VLNEETPSYTFILDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETA 145
           VLN++     F   DDG+NP QRFVSEDGVEN+RGR+LGG SM+NAGFYSR H+EFFET 
Sbjct: 91  VLNKQGLMNAFTDKDDGENPFQRFVSEDGVENLRGRILGGSSMINAGFYSRAHKEFFETQ 150

Query: 146 GV-DWDMELVEKAYEWVEETLVSRPNLTAWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIG 205
            + +WDME+V +AYEWVEETLVS PNL++WQ AFR  LLE GV  DNGF+LRHLVGTKIG
Sbjct: 151 EIIEWDMEMVREAYEWVEETLVSEPNLSSWQFAFRKALLEVGVDHDNGFELRHLVGTKIG 210

Query: 206 GSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTTLI 265
           GSIFDN+GNRHGAVELLNK   +NLKV +QATV+RI+F GLSA GVLYSDSKGK HT  I
Sbjct: 211 GSIFDNQGNRHGAVELLNKGESENLKVAVQATVKRILFSGLSANGVLYSDSKGKSHTAFI 270

Query: 266 HKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHLVHHQPHVGQYMTDNPRFSRSIIF 325
           H+KGEI LSAGAIGSPQLLLLSG+G  S+LSSL L L  HQPHVGQ+M+DNPRF  +I+ 
Sbjct: 271 HEKGEIILSAGAIGSPQLLLLSGVGSTSHLSSLNLPLFLHQPHVGQFMSDNPRFGVNIVL 330

Query: 326 PFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQSTSITSSLVIFVGKFSNV 385
           PF L  +T +VVG LEKN + +SL+S +PF   PS+ LLPPQSTS+  SLV+  GKFS V
Sbjct: 331 PFPLPTTTVEVVGILEKNTYFESLSSFIPFSIPPSFSLLPPQSTSLNMSLVLISGKFSKV 390

Query: 386 SSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGMRKVEDLLKTQTMERIKTQDLE 445
            S G L L NSSTD +++P+VRFNY+S   DL++C+ G+RK++DLL TQT+E IKT+DLE
Sbjct: 391 DSLGSLWL-NSSTDVRKSPMVRFNYFSHPRDLAQCIGGLRKIQDLLNTQTIENIKTKDLE 450

Query: 446 GNKGFQFMELPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRV 505
           G K  QF+ +P+PEN+ +D+ V E+CK TV T+WH+HGGC+VGKVVD  Y+V+GI+NLRV
Sbjct: 451 GKKTLQFLGIPLPENMADDTLVGEFCKRTVTTFWHFHGGCVVGKVVDGTYRVMGIENLRV 510

Query: 506 VDGSTFSDSPGTNPMATLMMLGR 528
           VDGSTFS+SPGTNPMAT+MMLGR
Sbjct: 511 VDGSTFSESPGTNPMATIMMLGR 532

BLAST of CSPI02G05810 vs. TAIR10
Match: AT1G73050.1 (AT1G73050.1 Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 478.0 bits (1229), Expect = 8.4e-135
Identity = 255/544 (46.88%), Postives = 347/544 (63.79%), Query Frame = 1

Query: 7   TFLPLILISIEFLSSHAIP--NQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATT 66
           T L ++L+      S+A P  N+   +M+F+ NA D   ++ YDYI++GGGT GCPLA T
Sbjct: 12  TALVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAAT 71

Query: 67  LSSKFSVLLLERGSDPNTYPFVLNEETPSYTFILDDDGQNPIQRFVSEDGVENIRGRVLG 126
           LS  F VLLLERG  P   P V++ +    T    ++  +P Q F+SE+GV N RGRVLG
Sbjct: 72  LSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLG 131

Query: 127 GGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRNTLLE 186
           G S +NAGFYSR  ++FFE +G+ WD+  V ++YEWVE  +V RP L  WQ A R+ LLE
Sbjct: 132 GSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLE 191

Query: 187 GGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIF-- 246
            GV P NGF L H VGTKIGGS FD  G RH + +LL  A   N++V + ATV+R++   
Sbjct: 192 VGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLAS 251

Query: 247 ------KGLSAVGVLYSDSKGKLHTTLIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSS 306
                   +SA+GV+Y D  G+ H  LI  +GE+ LSAGA+GSPQLL LSGIGP+SYLS+
Sbjct: 252 SPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLST 311

Query: 307 LKLHLVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPF-F 366
             + +   QPHVG ++ DNPR   SI+ P  +  S  QVVG  E    L++ ++ +PF  
Sbjct: 312 WGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFAS 371

Query: 367 PLPSYGLLPPQSTSITSSLVIFVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGD 426
           PL S  +  P S  +   +   + K     S G L L  +STD + NP+VRFNY+S   D
Sbjct: 372 PLHSVFIRAPAS-PLYVPVTTIMEKILGPVSIGLLRL--ASTDVRINPVVRFNYFSDPQD 431

Query: 427 LSRCVSGMRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVA 486
           L RCV+G RK+ ++L+++ M+    ++  GN+ F+F+  P+P +  ND  + ++C+ TV+
Sbjct: 432 LERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVS 491

Query: 487 TYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLG--------R 532
           T WHYHGG +VGKVVD + KVIG+ +LR+VDGSTF+ SPGTNP ATLMMLG        R
Sbjct: 492 TIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLR 551

BLAST of CSPI02G05810 vs. TAIR10
Match: AT1G12570.1 (AT1G12570.1 Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 385.6 bits (989), Expect = 5.7e-107
Identity = 230/551 (41.74%), Postives = 310/551 (56.26%), Query Frame = 1

Query: 18  FLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERG 77
           FL S    +       F+ +A   P    YDYI+IGGGT GCPLA TLS   SVLLLERG
Sbjct: 17  FLHSPICSSDKAPNYSFMRDATGSPTTSYYDYIIIGGGTAGCPLAATLSQNASVLLLERG 76

Query: 78  SDPNTYPFVLNEETPSYTFILDDDGQN-PIQRFVSEDGVENIRGRVLGGGSMVNAGFYSR 137
             P   P +      ++   L D  ++ P QRFVSEDGV N R RVLGGGS +NAGFY+R
Sbjct: 77  DSPYNNPNITR--LSAFGAALSDLSESSPSQRFVSEDGVINARARVLGGGSALNAGFYTR 136

Query: 138 GHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRNTLLEGGVVPDNGFDLR 197
              ++    G  WD  L  ++Y+WVE  +  +P +  WQ A R+ LLE G+VP+NGF   
Sbjct: 137 AGTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAGIVPNNGFTYD 196

Query: 198 HLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLS-----AVGVL 257
           H+ GTK GG+IFD  GNRH A +LL  A+PK + V++ ATV RI+F+        A GV+
Sbjct: 197 HINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTRGTTKPIANGVV 256

Query: 258 YSDSKGKLHTTLIHKKG---EIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHLVHHQPHV 317
           Y D  G+ H   + K+G   EI LSAG +GSPQLL+LSG+GP + L +  + +V  QPHV
Sbjct: 257 YRDRTGQAHRAYL-KEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHV 316

Query: 318 GQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSL---------------ASPLP 377
           GQ M DNP  +  +  P  +  S  +VVG   +  ++++                +S   
Sbjct: 317 GQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGSSGSSSTRD 376

Query: 378 FFPL--PSYGLLPPQSTSITSSLVIFVGKFSNVSSKGWL---HLNNSSTDAKENPIVRFN 437
           ++ +  P   LL   S +  SS   F G F      G L   HL   + + K+NPIV FN
Sbjct: 377 YYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLELKTRNPKDNPIVTFN 436

Query: 438 YYSQHGDLSRCVSGMRKVEDLLKTQTMERIKTQDLEGNKGFQF---MELPMPENLWN--- 497
           Y+    DL RCV G++ +E +++++   R K  D+     F++   +    P NL     
Sbjct: 437 YFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADV----SFEYLLNLTASTPVNLRPPRS 496

Query: 498 ------DSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGT 528
                   S EE+C++TV T WHYHGGC+VG+VVD +YKVIGI  LRV+D ST    PGT
Sbjct: 497 GPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMSTVGYCPGT 556

BLAST of CSPI02G05810 vs. TAIR10
Match: AT5G51930.1 (AT5G51930.1 Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 374.8 bits (961), Expect = 1.0e-103
Identity = 220/529 (41.59%), Postives = 307/529 (58.03%), Query Frame = 1

Query: 34  FVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEETPS 93
           F+ +A   P    +DYI+IGGGT GC LA TLS   SVL+LERG  P   P    +   S
Sbjct: 60  FMKDATLAPKNASFDYIIIGGGTAGCALAATLSQNASVLVLERGGSPYENP-TATDMGNS 119

Query: 94  YTFILDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDWDMEL 153
              +L++   +  Q F+SEDGV N R RVLGGGS++N GFYSR   ++ E A  +W+ME 
Sbjct: 120 VNTLLNNTPNSWSQLFISEDGVYNTRPRVLGGGSVINGGFYSRAGNDYVEEA--EWEMEE 179

Query: 154 VEKAYEWVEETLVSRPNLTAWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGN 213
           VE AYEWVE+ LV  P +  WQ AF++ LLE G  PDNGF   H+ GTKIGG+IFD  G+
Sbjct: 180 VEAAYEWVEKKLVFEPQVIEWQKAFKDGLLEAGESPDNGFTYDHIYGTKIGGTIFDRAGH 239

Query: 214 RHGAVELLNKANPKNLKVVIQATVQRIIFKGLSAVGVLYSDSKGKLHTTLIHKK--GEIF 273
           RH A  LL  ANP  + V + A+V +++F    A  VL+ D+ G  H   +  K   E+ 
Sbjct: 240 RHTAANLLEYANPNRIVVYLHASVHKVLFT-TEAYEVLFEDANGVFHKANLANKATNEVI 299

Query: 274 LSAGAIGSPQLLLLSGIGPKSYLSSLKLH-LVHHQPHVGQYMTDNPRFSRSIIFPFQLLA 333
           LSAGA+GSPQLL+LSG+GP  +L +  ++ LV  QP VGQ M DNP    +I  P  +  
Sbjct: 300 LSAGALGSPQLLMLSGVGPAVHLEAHGVNPLVLDQPMVGQGMADNPMNFVAIPSPQPVEL 359

Query: 334 STAQVVGTLEKNIHLQSLASPLPFFPLPSY---GLL-----PPQSTS---ITSSLVIFVG 393
           S  Q VG  + + +++ L+     F +      G+L        +TS   +T S+ + + 
Sbjct: 360 SLIQAVGITKFDSYIEGLSGLSLSFDITRRFFDGVLNLLNETSHTTSRKILTQSIAVLLK 419

Query: 394 KF-----------------SNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSG 453
            F                    +SKG + L N  T+ ++NP V FNYY +  DL++CV G
Sbjct: 420 SFDVKLEVRMNGGLIFQKVDGPASKGHMKLRN--TNPRDNPSVTFNYYQEPEDLNKCVKG 479

Query: 454 MRKVEDLLKTQTMERIKTQDLEGNKGFQFM-ELPM---PENLWNDSSVEEYCKNTVATYW 513
           +  +  ++ ++   + K   +   +    M  LP+   P ++ +  +++++C +TV + W
Sbjct: 480 LNTIIRMINSKAFSKYKYPGVTARELLNLMLALPINLRPRHVTSAFNLKQFCIDTVTSVW 539

Query: 514 HYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 528
           HYHGGC VGKVVD NYKV+GI  LRV+DGSTF  SPGTNP AT+MMLGR
Sbjct: 540 HYHGGCQVGKVVDKNYKVLGIDGLRVIDGSTFLKSPGTNPQATVMMLGR 582

BLAST of CSPI02G05810 vs. TAIR10
Match: AT3G56060.1 (AT3G56060.1 Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 373.6 bits (958), Expect = 2.2e-103
Identity = 233/583 (39.97%), Postives = 332/583 (56.95%), Query Frame = 1

Query: 1   MEYSIATFLPLILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP 60
           M + I  F   IL+++ F+   +  + +    +F+ +A   P    +DYI+IGGGT GC 
Sbjct: 1   MNFHIFRFFQFILVAV-FIFHDSCYSDEAGNYRFMKDATLAPKLSHFDYIIIGGGTAGCA 60

Query: 61  LATTLSSKFSVLLLERGSDPNTYPFVLNEETPSYTFILDDDGQNPIQRFVSEDGVENIRG 120
           LA TLS   +VL+LERG  P   P   +    + T +L+    +  Q F+SEDGV N R 
Sbjct: 61  LAATLSQNATVLVLERGGSPYDDPAATDIGNFANT-LLNITPNSWSQLFISEDGVFNSRA 120

Query: 121 RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN 180
           RVLGGG+++NAGFYSR  ++F   AG  W+ + VE AYEWVE+ +V  P +  WQ+AFR+
Sbjct: 121 RVLGGGTVINAGFYSRAEEDFVAEAG--WERDEVEAAYEWVEKKVVFEPPVNKWQSAFRD 180

Query: 181 TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI 240
            LLE GV P NGF   H+VGTK GG+IFD  G+RH A  LL  ANP  + V + A+V +I
Sbjct: 181 GLLEAGVTPYNGFTYEHIVGTKFGGTIFDRDGHRHTAANLLEYANPNMIVVYLHASVHKI 240

Query: 241 IF--KG---LSAVGVLYSDSKGKLH----TTLIHKKGEIFLSAGAIGSPQLLLLSGIGPK 300
           +F  KG     A GV++ D+ G  +     T      E+ LSAGAI SPQLL+LSG+GP 
Sbjct: 241 LFTIKGNQRPKAYGVIFLDANGVSYKAELATQDSTMSEVILSAGAIASPQLLMLSGVGPA 300

Query: 301 SYLSSLKLH-LVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQ---- 360
           ++L++ +++ ++  QP VGQ M DNP     I  P  +  S  Q VG  +   +++    
Sbjct: 301 AHLAAYRVNPVIVDQPMVGQGMGDNPMNPVFIPSPEPVEVSLVQAVGITKFGSYIEGGSA 360

Query: 361 ---SLASPLPFFP----LPSYGLLPPQSTS-----------ITSSLVIFVGKFSNVSSKG 420
              S++    FF     L     LP QS S           +T+   + + K +   S+G
Sbjct: 361 LSLSISLTRSFFDGVLNLLKKTKLPTQSISKFFKSLDLTLNVTTKAGVIIQKVNGPLSRG 420

Query: 421 WLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGMRKVEDLLKTQTMERIKTQDLEGNKG 480
            L L N++ D  +NP V FNY+    DL++CV G+  +  ++ ++   + K   L   +G
Sbjct: 421 HLELRNTNPD--DNPSVTFNYFKDPEDLNKCVEGLSTIIKVIDSKGYSKYK-YPLASARG 480

Query: 481 FQFMELPMPENL-----WNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLR 539
              + L +P NL      +   +E+YC +TV T +HYHGGC VGKVVD+NYKV+G+  LR
Sbjct: 481 LLNLILALPTNLRPRHITSTFDLEQYCIDTVMTIYHYHGGCQVGKVVDNNYKVLGVDALR 540

BLAST of CSPI02G05810 vs. TAIR10
Match: AT1G72970.1 (AT1G72970.1 Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 370.9 bits (951), Expect = 1.5e-102
Identity = 233/547 (42.60%), Postives = 309/547 (56.49%), Query Frame = 1

Query: 30  SYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSKFSVLLLERGSDPNT---YPFV 89
           S   F  N  D      YDYIVIGGGT GCPLA TLS  FSVL+LERG  P T     F+
Sbjct: 50  SSSSFSSNGQDS----SYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFL 109

Query: 90  LNEETPSYTFILDDDGQNPIQRFVSEDGVENIRGRVLGGGSMVNAGFYSRGHQEFFETAG 149
            N     +  + D    +  Q FVS DGV N R RVLGGGS +NAGFYSR    F + AG
Sbjct: 110 RN----FHIGLADISASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG 169

Query: 150 VDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRNTLLEGGVVPDNGFDLRHLVGTKIGGS 209
             WD +LV+++Y WVE  +V +P LT WQ A R++LLE GV P NGF   H+ GTKIGG+
Sbjct: 170 --WDPKLVKESYPWVEREIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGT 229

Query: 210 IFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSA----VGVLYSDSKGKLHTT 269
           IFD  G RH A ELL  ANP+ L+V+I ATVQ+I+F          GV++ D KG  H  
Sbjct: 230 IFDRFGRRHTAAELLAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQA 289

Query: 270 LI-HKKG-EIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHLVHHQPHVGQYMTDNPR--- 329
           L+ ++KG E+ LS+GAIGSPQ+L+LSGIGPK  L  LK+ +V    HVG+ M DNP    
Sbjct: 290 LLSNRKGSEVILSSGAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTI 349

Query: 330 -------FSRSIIFPFQLLASTAQV-----VGTLEKNIH----LQSLASPLPFFPLPSYG 389
                    +S+I    +      V      G   ++IH    + S  + L F  +P+  
Sbjct: 350 LVPSKAPIEQSLIQTVGITKMGVYVEASTGFGQSPESIHTHYGIMSNKNEL-FSTIPAKQ 409

Query: 390 LLPPQSTS-ITSSLV---------IFVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYS 449
             P  + + IT +             + K +   S+G L L N++ D  +NP V FNY+ 
Sbjct: 410 RRPEATQAYITRNKYQLHEAFNGSFILEKLAYPISRGHLSLVNTNVD--DNPSVTFNYFK 469

Query: 450 QHGDLSRCVSGMRKVEDLLKT-----------QTMERIKTQDLEGNKGFQFMELPMPENL 509
              DL RCV  +R V  ++ +           Q + ++ +  ++ N   +      P+ L
Sbjct: 470 HPVDLQRCVEAIRLVSKVVTSNRFLNYTQCDKQNVHKMLSLSVKANINLR------PKQL 529

Query: 510 WNDSSVEEYCKNTVATYWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMA 528
            +  S+ ++CK+TV T WHYHGGCLVGKVV  N KV+G+  LRV+DGSTF +SPGTNP A
Sbjct: 530 NDTKSMAQFCKDTVVTIWHYHGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQA 577

BLAST of CSPI02G05810 vs. NCBI nr
Match: gi|778667413|ref|XP_011648923.1| (PREDICTED: (R)-mandelonitrile lyase 3-like isoform X2 [Cucumis sativus])

HSP 1 Score: 1056.6 bits (2731), Expect = 1.6e-305
Identity = 522/527 (99.05%), Postives = 524/527 (99.43%), Query Frame = 1

Query: 1   MEYSIATFLPLILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP 60
           MEYSIATFLPLILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP
Sbjct: 1   MEYSIATFLPLILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP 60

Query: 61  LATTLSSKFSVLLLERGSDPNTYPFVLNEETPSYTFILDDDGQNPIQRFVSEDGVENIRG 120
           LATTLSSKFSVLLLERGSDPNTYPFVLNEET SYTFILDDDGQNPIQRFVSEDGVENIRG
Sbjct: 61  LATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRG 120

Query: 121 RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN 180
           RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN
Sbjct: 121 RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN 180

Query: 181 TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI 240
           TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI
Sbjct: 181 TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI 240

Query: 241 IFKGLSAVGVLYSDSKGKLHTTLIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLH 300
           IFKGLSAVGVLYSDSKGKLHT LIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLH
Sbjct: 241 IFKGLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLH 300

Query: 301 LVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSY 360
           LVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSY
Sbjct: 301 LVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSY 360

Query: 361 GLLPPQSTSITSSLVIFVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCV 420
           GLLPPQSTSITSSLVI VGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGD+SRCV
Sbjct: 361 GLLPPQSTSITSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDISRCV 420

Query: 421 SGMRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVATYWHY 480
           SG+RKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVATYWHY
Sbjct: 421 SGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVATYWHY 480

Query: 481 HGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 528
           HGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR
Sbjct: 481 HGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 527

BLAST of CSPI02G05810 vs. NCBI nr
Match: gi|778667410|ref|XP_011648922.1| (PREDICTED: (R)-mandelonitrile lyase 3-like isoform X1 [Cucumis sativus])

HSP 1 Score: 1023.1 bits (2644), Expect = 2.0e-295
Identity = 504/527 (95.64%), Postives = 511/527 (96.96%), Query Frame = 1

Query: 1   MEYSIATFLPLILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP 60
           M   I  F  + +  +  ++SH IPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP
Sbjct: 5   MANQILLFTLISIFQLGLIASHTIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCP 64

Query: 61  LATTLSSKFSVLLLERGSDPNTYPFVLNEETPSYTFILDDDGQNPIQRFVSEDGVENIRG 120
           LATTLSSKFSVLLLERGSDPNTYPFVLNEET SYTFILDDDGQNPIQRFVSEDGVENIRG
Sbjct: 65  LATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYTFILDDDGQNPIQRFVSEDGVENIRG 124

Query: 121 RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN 180
           RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN
Sbjct: 125 RVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFRN 184

Query: 181 TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI 240
           TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI
Sbjct: 185 TLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRI 244

Query: 241 IFKGLSAVGVLYSDSKGKLHTTLIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLH 300
           IFKGLSAVGVLYSDSKGKLHT LIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLH
Sbjct: 245 IFKGLSAVGVLYSDSKGKLHTALIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLH 304

Query: 301 LVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSY 360
           LVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSY
Sbjct: 305 LVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSY 364

Query: 361 GLLPPQSTSITSSLVIFVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCV 420
           GLLPPQSTSITSSLVI VGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGD+SRCV
Sbjct: 365 GLLPPQSTSITSSLVIIVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDISRCV 424

Query: 421 SGMRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVATYWHY 480
           SG+RKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVATYWHY
Sbjct: 425 SGVRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVATYWHY 484

Query: 481 HGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 528
           HGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR
Sbjct: 485 HGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 531

BLAST of CSPI02G05810 vs. NCBI nr
Match: gi|659069812|ref|XP_008451913.1| (PREDICTED: (R)-mandelonitrile lyase 3-like [Cucumis melo])

HSP 1 Score: 1010.7 bits (2612), Expect = 1.0e-291
Identity = 497/528 (94.13%), Postives = 514/528 (97.35%), Query Frame = 1

Query: 1   MEYSIATFLPL-ILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGC 60
           ME+S+ATFL L ILISIEF+SSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGC
Sbjct: 1   MEHSLATFLLLLILISIEFISSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGC 60

Query: 61  PLATTLSSKFSVLLLERGSDPNTYPFVLNEETPSYTFILDDDGQNPIQRFVSEDGVENIR 120
           PLATTLSSKFSVLLLERGSDPNTYPFVLNEET SY FIL+DDGQNPIQRFVSEDGVENIR
Sbjct: 61  PLATTLSSKFSVLLLERGSDPNTYPFVLNEETLSYVFILEDDGQNPIQRFVSEDGVENIR 120

Query: 121 GRVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQAAFR 180
           GRVLGGGSMVNAGFYSRGH+EFFETAGVDW+MELVEKAYEWVEETLVSRPNL+AWQ AFR
Sbjct: 121 GRVLGGGSMVNAGFYSRGHREFFETAGVDWEMELVEKAYEWVEETLVSRPNLSAWQVAFR 180

Query: 181 NTLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQR 240
           NTLLEGGVVPDNGFDLRHL+GTK GGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQR
Sbjct: 181 NTLLEGGVVPDNGFDLRHLMGTKTGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQR 240

Query: 241 IIFKGLSAVGVLYSDSKGKLHTTLIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKL 300
           IIF GLSAVGVLYSDSKGKLHT LIHKKGEI LSAGAIGSPQLLLLSGIGPKSYLSSLKL
Sbjct: 241 IIFNGLSAVGVLYSDSKGKLHTALIHKKGEILLSAGAIGSPQLLLLSGIGPKSYLSSLKL 300

Query: 301 HLVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPS 360
           H+VHHQPHVGQ+MTDNPRFSRSI+FPFQLLAS+AQVVGTLEKNIHLQSLASPLPF+PLPS
Sbjct: 301 HVVHHQPHVGQFMTDNPRFSRSIVFPFQLLASSAQVVGTLEKNIHLQSLASPLPFYPLPS 360

Query: 361 YGLLPPQSTSITSSLVIFVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRC 420
           YGLLPPQSTSIT SLV+FVGKFSNVSSKGWLHLNNSSTD+KE+PIVRFNYYSQH DLSRC
Sbjct: 361 YGLLPPQSTSITPSLVVFVGKFSNVSSKGWLHLNNSSTDSKESPIVRFNYYSQHDDLSRC 420

Query: 421 VSGMRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVATYWH 480
           V G+RKVEDLLKTQTMER KTQDLEGNKGFQFM LPMPENLWNDSSVEEYCKNTVATYWH
Sbjct: 421 VRGVRKVEDLLKTQTMERFKTQDLEGNKGFQFMGLPMPENLWNDSSVEEYCKNTVATYWH 480

Query: 481 YHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 528
           YHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR
Sbjct: 481 YHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 528

BLAST of CSPI02G05810 vs. NCBI nr
Match: gi|659069842|ref|XP_008452065.1| (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo])

HSP 1 Score: 817.4 bits (2110), Expect = 1.7e-233
Identity = 401/520 (77.12%), Postives = 446/520 (85.77%), Query Frame = 1

Query: 9   LPLILISIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGTTGCPLATTLSSK 68
           L + +  ++ LSS AIPNQD+SYMKFVHNA D P +E+YDYI+IGGGT GCPLATTLSSK
Sbjct: 13  LTISIFPLQLLSSEAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSK 72

Query: 69  FSVLLLERGSDPNTYPFVLNEETPSYTFILDDDGQNPIQRFVSEDGVENIRGRVLGGGSM 128
           FS+L+LERGSDPN YP VLN +  +  F   DDG+NP Q FVSEDGVENIRGRVLGGGSM
Sbjct: 73  FSILVLERGSDPNKYPSVLNAQGINNAFAAGDDGKNPFQYFVSEDGVENIRGRVLGGGSM 132

Query: 129 VNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLT-AWQAAFRNTLLEGGV 188
           VNAGFYSRGH+EFFE AGVDWDMELVEKAYEWVEET+VS+P L  AWQAAFR+ LLEGGV
Sbjct: 133 VNAGFYSRGHREFFENAGVDWDMELVEKAYEWVEETVVSQPILNNAWQAAFRSALLEGGV 192

Query: 189 VPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQATVQRIIFKGLSA 248
           VPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANP N+KV IQATVQ+I+F GLSA
Sbjct: 193 VPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPTNIKVAIQATVQKILFSGLSA 252

Query: 249 VGVLYSDSKGKLHTTLIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSSLKLHLVHHQPH 308
            GV YSDSKGKLHT  IHK GEI +SAGAIGSPQLLLLSGIGPKS+LSSLKL +V HQPH
Sbjct: 253 TGVSYSDSKGKLHTASIHKNGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPH 312

Query: 309 VGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFPLPSYGLLPPQS 368
           VGQ+MTDNPRF  +I+ PFQLL S  +VVG L++NI++QS A P  F   P++ LLPPQS
Sbjct: 313 VGQFMTDNPRFGTTIVLPFQLLPSAGKVVGILQENIYIQSFAGPSTFLVPPAFSLLPPQS 372

Query: 369 TSITSSLVIFVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDLSRCVSGMRKVE 428
           TSI  +L  FVGKFS+V+SKGWLHL NSS D K++PIVRFNYYS H DL RCV G+RKV 
Sbjct: 373 TSINPTLASFVGKFSDVNSKGWLHL-NSSLDVKKSPIVRFNYYSHHDDLDRCVRGVRKVG 432

Query: 429 DLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVATYWHYHGGCLVG 488
           DLLKTQT+E+IKTQDLEG K F F+ LP+ ENLWNDSSV+EYCK TV TYWHYHGGCLVG
Sbjct: 433 DLLKTQTIEKIKTQDLEGEKAFMFLGLPLTENLWNDSSVKEYCKKTVTTYWHYHGGCLVG 492

Query: 489 KVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 528
           KVVD NYKVIGI+NLRVVDGSTFS SPGTNPMATLMMLGR
Sbjct: 493 KVVDGNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGR 531

BLAST of CSPI02G05810 vs. NCBI nr
Match: gi|700205993|gb|KGN61112.1| (hypothetical protein Csa_2G055000 [Cucumis sativus])

HSP 1 Score: 800.8 bits (2067), Expect = 1.6e-228
Identity = 398/531 (74.95%), Postives = 453/531 (85.31%), Query Frame = 1

Query: 1   MEYSIATFLPLILI----SIEFLSSHAIPNQDISYMKFVHNANDQPIQEKYDYIVIGGGT 60
           ME+S  T L L L+     ++  SSHAIPNQD+SYMKFVHNA D P +E+YDYI+IGGGT
Sbjct: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT 60

Query: 61  TGCPLATTLSSKFSVLLLERGSDPNTYPFVLNEETPSYTFILDDDGQNPIQRFVSEDGVE 120
            GCPLATTLSSKFSVLLLERGSDPN YP VL+E+     F   DDG+NP QRFVSEDGVE
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVE 120

Query: 121 NIRGRVLGGGSMVNAGFYSRGHQEFFETAGVDWDMELVEKAYEWVEETLVSRPNLTAWQA 180
           NIRGRVLGGGSMVNAGFYSRGH+EFF +AGVDWDMELVEKAYEWVEET+VS+P L AWQ+
Sbjct: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 181 AFRNTLLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPKNLKVVIQAT 240
           AFR++LLEGGVVPD+GFDLRHLVGTK GGSIFDNKGNRHGAVELLNKANP N+KV I+AT
Sbjct: 181 AFRSSLLEGGVVPDSGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240

Query: 241 VQRIIFKGLSAVGVLYSDSKGKLHTTLIHKKGEIFLSAGAIGSPQLLLLSGIGPKSYLSS 300
           VQRI+F GLSA GVLYSDSKGKLH  +I KKGEI +SAGAIGSPQLLLLSGIGPKS+LSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS 300

Query: 301 LKLHLVHHQPHVGQYMTDNPRFSRSIIFPFQLLASTAQVVGTLEKNIHLQSLASPLPFFP 360
           LKL +V HQP+VGQ M+DNPRF  +II PF +L S+ +VVG L+ NI++QS+ASP P   
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI 360

Query: 361 LPSYGLLPPQSTSITSSLVIFVGKFSNVSSKGWLHLNNSSTDAKENPIVRFNYYSQHGDL 420
              + LLPPQ+TSI  +L +FVGKFS V S+G L L NSST+ K++PIV FNYYS   DL
Sbjct: 361 PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRL-NSSTNVKKSPIVGFNYYSHPDDL 420

Query: 421 SRCVSGMRKVEDLLKTQTMERIKTQDLEGNKGFQFMELPMPENLWNDSSVEEYCKNTVAT 480
            RCV G+RK+ DLLKT+TME+IKT++LEGNKGF+F+ +P+PENLWNDSSVEEYCK TV T
Sbjct: 421 GRCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTT 480

Query: 481 YWHYHGGCLVGKVVDDNYKVIGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 528
           YWHYHGGCLVGKVVD NYKVIGIKNLRVVDGSTFS+SPGTNPMATLMMLGR
Sbjct: 481 YWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 530

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
MDL3_PRUSE7.9e-14347.11(R)-mandelonitrile lyase 3 OS=Prunus serotina GN=MDL3 PE=2 SV=1[more]
MDL1_PRUSE1.4e-13946.44(R)-mandelonitrile lyase 1 OS=Prunus serotina GN=MDL1 PE=1 SV=1[more]
MDL1_PRUDU4.1e-13946.87(R)-mandelonitrile lyase 1 OS=Prunus dulcis GN=MDL1 PE=1 SV=1[more]
MDL2_PRUSE9.1e-13946.21(R)-mandelonitrile lyase 2 OS=Prunus serotina GN=MDL2 PE=2 SV=1[more]
MDL2_PRUDU1.2e-13845.89(R)-mandelonitrile lyase 2 OS=Prunus dulcis GN=MDL2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LMB8_CUCSA1.1e-30599.05Uncharacterized protein OS=Cucumis sativus GN=Csa_2G055040 PE=3 SV=1[more]
A0A0A0LK76_CUCSA1.1e-22874.95Uncharacterized protein OS=Cucumis sativus GN=Csa_2G055000 PE=3 SV=1[more]
A0A0A0LK79_CUCSA7.3e-22874.95Uncharacterized protein OS=Cucumis sativus GN=Csa_2G055050 PE=3 SV=1[more]
A0A0A0LZU8_CUCSA2.0e-22273.53Uncharacterized protein OS=Cucumis sativus GN=Csa_1G627390 PE=3 SV=1[more]
A0A0A0LMB3_CUCSA9.9e-20168.39Uncharacterized protein OS=Cucumis sativus GN=Csa_2G050000 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G73050.18.4e-13546.88 Glucose-methanol-choline (GMC) oxidoreductase family protein[more]
AT1G12570.15.7e-10741.74 Glucose-methanol-choline (GMC) oxidoreductase family protein[more]
AT5G51930.11.0e-10341.59 Glucose-methanol-choline (GMC) oxidoreductase family protein[more]
AT3G56060.12.2e-10339.97 Glucose-methanol-choline (GMC) oxidoreductase family protein[more]
AT1G72970.11.5e-10242.60 Glucose-methanol-choline (GMC) oxidoreductase family protein[more]
Match NameE-valueIdentityDescription
gi|778667413|ref|XP_011648923.1|1.6e-30599.05PREDICTED: (R)-mandelonitrile lyase 3-like isoform X2 [Cucumis sativus][more]
gi|778667410|ref|XP_011648922.1|2.0e-29595.64PREDICTED: (R)-mandelonitrile lyase 3-like isoform X1 [Cucumis sativus][more]
gi|659069812|ref|XP_008451913.1|1.0e-29194.13PREDICTED: (R)-mandelonitrile lyase 3-like [Cucumis melo][more]
gi|659069842|ref|XP_008452065.1|1.7e-23377.12PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo][more]
gi|700205993|gb|KGN61112.1|1.6e-22874.95hypothetical protein Csa_2G055000 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000172GMC_OxRdtase_N
IPR007867GMC_OxRtase_C
IPR023753FAD/NAD-binding_dom
IPR000172GMC_OxRdtase_N
IPR007867GMC_OxRtase_C
IPR023753FAD/NAD-binding_dom
Vocabulary: Molecular Function
TermDefinition
GO:0016614oxidoreductase activity, acting on CH-OH group of donors
GO:0050660flavin adenine dinucleotide binding
GO:0016491oxidoreductase activity
GO:0016614oxidoreductase activity, acting on CH-OH group of donors
GO:0050660flavin adenine dinucleotide binding
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors
molecular_function GO:0016491 oxidoreductase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G05810.2CSPI02G05810.2mRNA
CSPI02G05810.1CSPI02G05810.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 47..316
score: 1.4
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00623GMC_OXRED_1coord: 120..143
scor
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 275..289
scor
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 386..527
score: 1.2
IPR023753FAD/NAD(P)-binding domainGENE3DG3DSA:3.50.50.60coord: 396..410
score: 1.6E-86coord: 478..537
score: 1.6E-86coord: 212..323
score: 1.6E-86coord: 36..166
score: 1.6
IPR023753FAD/NAD(P)-binding domainunknownSSF51905FAD/NAD(P)-binding domaincoord: 482..536
score: 6.6E-49coord: 30..309
score: 6.6
NoneNo IPR availablePANTHERPTHR11552GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASEcoord: 23..537
score: 4.2E
NoneNo IPR availablePANTHERPTHR11552:SF82(R)-MANDELONITRILE LYASE-LIKEcoord: 23..537
score: 4.2E
NoneNo IPR availableunknownSSF54373FAD-linked reductases, C-terminal domaincoord: 373..483
score: 9.81

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI02G05810Cla022835Watermelon (97103) v1cpiwmB166
CSPI02G05810Csa2G055000Cucumber (Chinese Long) v2cpicuB065
CSPI02G05810ClCG11G012330Watermelon (Charleston Gray)cpiwcgB110
The following gene(s) are paralogous to this gene:

None