CSPI02G05770 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI02G05770
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	(R)-mandelonitrile lyase-like
Location	Chr2 : 4226449 .. 4227283 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGTTTGTACATAATGCTACCGATTTACCAACAAAAGAAGAATTTGACTACATAATTATAGGAGGTGGAACTGCAGGGTGTCCATTAGCTACAACATTATCATCAAAGTTCTCAGTTCTCCTTCTTGAAAGAGGCAGTGACCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTCTATTGAAGGCTTTTGATACAGAAGATGATGGCAAAACTCCCTTCCAACGTTTCATCTCGGAGGACGGCTTAGAGAACATTCGAGGGCGAGTCCTCGGCGGTGGAAGCATGGTCAATGCCGGGTTCTATTCAAGGGGACATAAAGAGTTTTTTGAAAGTGCAGGTGTTGATTGGAACATGGAATTGGTGGAGAAAGCTTATGAATGGATAGAAGAGAGTGTGGTATCACAACCGATTTTGAAAGATTGGCAATCTGCTTTTAGAAGTGAAGAAGAATCCCATTGTTCGGTTCAATTATTATTCCCATCCTGATGATCTTGCTCGATGTGTTAAAGGTGTAAGAAAATTGGGAGATGTGTTTAAAACTCCAACGTTGGAAAAGATTAAGACCCAGAATTTGAAGGGTAATAAAGGATTTATGTTTTTGGGGCTTCCATTGCCGGGAAATTTGTGGAATGATAGCGCTGTTGAAGAATATTGTAAGAAAACGGTGGCTACTTATTGGCATTATCATGGAGGATGTTTGGTCGGAAAAGTTGTGGACGGTAATTATAAAGTGATCGAAACTGAAAATTTACGTGTAGTTGATGGCTCCACTTTCTCTGTCTCGCCAGGAACTAATCCTATGGCCACCCTCATGATGCTTGGCCCGTGA

mRNA sequence

ATGAAGTTTGTACATAATGCTACCGATTTACCAACAAAAGAAGAATTTGACTACATAATTATAGGAGGTGGAACTGCAGGGTGTCCATTAGCTACAACATTATCATCAAAGTTCTCAGTTCTCCTTCTTGAAAGAGGCAGTGACCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTCTATTGAAGGCTTTTGATACAGAAGATGATGGCAAAACTCCCTTCCAACGTTTCATCTCGGAGGACGGCTTAGAGAACATTCGAGGGCGAGTCCTCGGCGGTGGAAGCATGGTCAATGCCGGGTTCTATTCAAGGGGACATAAAGAGTTTTTTGAAAGTGCAGAAGTGAAGAAGAATCCCATTGTTCGGTTCAATTATTATTCCCATCCTGATGATCTTGCTCGATGTGTTAAAGGTGTAAGAAAATTGGGAGATGTGTTTAAAACTCCAACGTTGGAAAAGATTAAGACCCAGAATTTGAAGGGTAATAAAGGATTTATGTTTTTGGGGCTTCCATTGCCGGGAAATTTGTGGAATGATAGCGCTGTTGAAGAATATTGTAAGAAAACGGTGGCTACTTATTGGCATTATCATGGAGGATGTTTGGTCGGAAAAGTTGTGGACGGTAATTATAAAGTGATCGAAACTGAAAATTTACGTGTAGTTGATGGCTCCACTTTCTCTGTCTCGCCAGGAACTAATCCTATGGCCACCCTCATGATGCTTGGCCCGTGA

Coding sequence (CDS)

ATGAAGTTTGTACATAATGCTACCGATTTACCAACAAAAGAAGAATTTGACTACATAATTATAGGAGGTGGAACTGCAGGGTGTCCATTAGCTACAACATTATCATCAAAGTTCTCAGTTCTCCTTCTTGAAAGAGGCAGTGACCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTCTATTGAAGGCTTTTGATACAGAAGATGATGGCAAAACTCCCTTCCAACGTTTCATCTCGGAGGACGGCTTAGAGAACATTCGAGGGCGAGTCCTCGGCGGTGGAAGCATGGTCAATGCCGGGTTCTATTCAAGGGGACATAAAGAGTTTTTTGAAAGTGCAGAAGTGAAGAAGAATCCCATTGTTCGGTTCAATTATTATTCCCATCCTGATGATCTTGCTCGATGTGTTAAAGGTGTAAGAAAATTGGGAGATGTGTTTAAAACTCCAACGTTGGAAAAGATTAAGACCCAGAATTTGAAGGGTAATAAAGGATTTATGTTTTTGGGGCTTCCATTGCCGGGAAATTTGTGGAATGATAGCGCTGTTGAAGAATATTGTAAGAAAACGGTGGCTACTTATTGGCATTATCATGGAGGATGTTTGGTCGGAAAAGTTGTGGACGGTAATTATAAAGTGATCGAAACTGAAAATTTACGTGTAGTTGATGGCTCCACTTTCTCTGTCTCGCCAGGAACTAATCCTATGGCCACCCTCATGATGCTTGGCCCGTGA

Homology

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BLAST of CSPI02G05770 vs. Swiss-Prot
Match: MDLL_ARATH ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.5e-39
Identity = 70/131 (53.44%), Postives = 97/131 (74.05%), Query Frame = 1


Query: 113 SAEVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLP 172
           S +V+ NP+VRFNY+S P DL RCV G RK+G++ ++  ++    +   GN+ F F+G P
Sbjct: 409 STDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAP 468

Query: 173 LPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPG 232
           LP +  ND  + ++C++TV+T WHYHGG +VGKVVD + KVI   +LR+VDGSTF++SPG
Sbjct: 469 LPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPG 528

Query: 233 TNPMATLMMLG 244
           TNP ATLMMLG
Sbjct: 529 TNPQATLMMLG 539

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BLAST of CSPI02G05770 vs. Swiss-Prot
Match: MDL1_PRUSE ((R)-mandelonitrile lyase 1 OS=Prunus serotina GN=MDL1 PE=1 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.8e-37
Identity = 69/144 (47.92%), Postives = 102/144 (70.83%), Query Frame = 1


Query: 100 AGFYSRGHKEFFESAEVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQN 159
           AG  S G      S+ V+ +P V+FNYYS+P DL+ CV G++K+G++  T  L+  K ++
Sbjct: 390 AGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHCVSGMKKIGELLSTDALKPYKVED 449

Query: 160 LKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENL 219
           L G +GF  LG+PLP +  +D+A E +C+++VA+YWHYHGGCLVGKV+DG+++V   + L
Sbjct: 450 LPGIEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGIDAL 509

Query: 220 RVVDGSTFSVSPGTNPMATLMMLG 244
           RVVDGSTF  +P ++P    +MLG
Sbjct: 510 RVVDGSTFPYTPASHPQGFYLMLG 533

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BLAST of CSPI02G05770 vs. Swiss-Prot
Match: MDL3_PRUSE ((R)-mandelonitrile lyase 3 OS=Prunus serotina GN=MDL3 PE=2 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 3.4e-36
Identity = 70/143 (48.95%), Postives = 96/143 (67.13%), Query Frame = 1


Query: 101 GFYSRGHKEFFESAEVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNL 160
           G  S G      S++V+  P V+FNYYS+  DL+ CV G++KLG+V  T  LE  K ++L
Sbjct: 392 GPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTDLSHCVSGMKKLGEVLSTDALEPYKVEDL 451

Query: 161 KGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLR 220
            G  GF  LG+PLP N  +D+A E +C+++VA+YWHYHGGCLVGKV+D  ++V     LR
Sbjct: 452 PGIDGFNILGIPLPENQTDDAAFETFCRESVASYWHYHGGCLVGKVLDDGFRVTGINALR 511

Query: 221 VVDGSTFSVSPGTNPMATLMMLG 244
           VVDGSTF  +P ++P    +MLG
Sbjct: 512 VVDGSTFPSTPASHPQGFYLMLG 534

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BLAST of CSPI02G05770 vs. Swiss-Prot
Match: MDL2_PRUDU ((R)-mandelonitrile lyase 2 OS=Prunus dulcis GN=MDL2 PE=1 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 4.5e-36
Identity = 68/144 (47.22%), Postives = 100/144 (69.44%), Query Frame = 1


Query: 100 AGFYSRGHKEFFESAEVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQN 159
           AG  S G      S+ V+ +P V+FNYYS+  DL+ CV G++K+G++  T  L+  K ++
Sbjct: 390 AGPLSYGSLTLKSSSNVRVSPNVKFNYYSNLTDLSHCVSGMKKIGELLSTDALKPYKVED 449

Query: 160 LKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENL 219
           L G +GF  LG+PLP +  +D+A E +C+++VA+YWHYHGGCLVGKV+DG+++V     L
Sbjct: 450 LPGVEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGINAL 509

Query: 220 RVVDGSTFSVSPGTNPMATLMMLG 244
           RVVDGSTF  +P ++P    +MLG
Sbjct: 510 RVVDGSTFPYTPASHPQGFYLMLG 533

[Close]

BLAST of CSPI02G05770 vs. Swiss-Prot
Match: MDL1_PRUDU ((R)-mandelonitrile lyase 1 OS=Prunus dulcis GN=MDL1 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 7.2e-34
Identity = 66/143 (46.15%), Postives = 95/143 (66.43%), Query Frame = 1


Query: 101 GFYSRGHKEFFESAEVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNL 160
           G  S G      S++V+  P ++FNYYS+  DLA CV G++KLGD+ +T  LE  K +++
Sbjct: 392 GPLSHGSVTLNSSSDVRIAPNIKFNYYSNSTDLANCVSGMKKLGDLLRTKALEPYKARDV 451

Query: 161 KGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLR 220
            G  GF +LG+PLP N  +D++ E +C   VA+YWHYHGG LVGKV+D +++V+  + LR
Sbjct: 452 LGIDGFNYLGVPLPENQTDDASFETFCLDNVASYWHYHGGSLVGKVLDDSFRVMGIKALR 511

Query: 221 VVDGSTFSVSPGTNPMATLMMLG 244
           VVD STF   P ++P    +MLG
Sbjct: 512 VVDASTFPYEPNSHPQGFYLMLG 534

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BLAST of CSPI02G05770 vs. TrEMBL
Match: A0A0A0LZU8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G627390 PE=3 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.0e-71
Identity = 129/141 (91.49%), Postives = 135/141 (95.74%), Query Frame = 1


Query: 103 YSRGHKEFFESAEVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKG 162
           +S+G  +   S +VKK+PIV+FNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKG
Sbjct: 390 HSKGSLKLNSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKG 449

Query: 163 NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVV 222
           NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVV
Sbjct: 450 NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVV 509

Query: 223 DGSTFSVSPGTNPMATLMMLG 244
           DGSTFSVSPGTNPMATLMMLG
Sbjct: 510 DGSTFSVSPGTNPMATLMMLG 530

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BLAST of CSPI02G05770 vs. TrEMBL
Match: A0A0A0LH79_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G055010 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.3e-69
Identity = 124/141 (87.94%), Postives = 134/141 (95.04%), Query Frame = 1


Query: 103 YSRGHKEFFESAEVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKG 162
           +S+G  +   S +VKK+PIV+FNYYSHPDDLARCVKGVRKLGDVFKTPT+EKIKTQ+LKG
Sbjct: 74  HSKGSLKLNSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLKG 133

Query: 163 NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVV 222
           NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVI TENLRVV
Sbjct: 134 NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIGTENLRVV 193

Query: 223 DGSTFSVSPGTNPMATLMMLG 244
           DGSTFS+SPGTNPMAT+MMLG
Sbjct: 194 DGSTFSISPGTNPMATVMMLG 214

[Close]

BLAST of CSPI02G05770 vs. TrEMBL
Match: A0A0A0LK76_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G055000 PE=3 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 2.2e-61
Identity = 112/141 (79.43%), Postives = 122/141 (86.52%), Query Frame = 1


Query: 103 YSRGHKEFFESAEVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKG 162
           +S G      S  VKK+PIV FNYYSHPDDL RCVKGVRK+GD+ KT T+EKIKT+NL+G
Sbjct: 389 HSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLLKTRTMEKIKTKNLEG 448

Query: 163 NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVV 222
           NKGF FLG+PLP NLWNDS+VEEYCKKTV TYWHYHGGCLVGKVVDGNYKVI  +NLRVV
Sbjct: 449 NKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNLRVV 508

Query: 223 DGSTFSVSPGTNPMATLMMLG 244
           DGSTFS SPGTNPMATLMMLG
Sbjct: 509 DGSTFSESPGTNPMATLMMLG 529

[Close]

BLAST of CSPI02G05770 vs. TrEMBL
Match: A0A0A0LK79_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G055050 PE=3 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 5.9e-59
Identity = 109/141 (77.30%), Postives = 123/141 (87.23%), Query Frame = 1


Query: 103 YSRGHKEFFESAEVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKG 162
           +S G      S +VK++PIVRFNYYSHPDDLARCV+GVRK+GD+ KTPT+EKIKTQ+L+G
Sbjct: 389 HSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVRGVRKVGDLLKTPTMEKIKTQDLEG 448

Query: 163 NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVV 222
           NK F FLGL LP NL ND+AVEEYC+KTV TYWHYHGGCLVGKVVD N+KVI  +NLRVV
Sbjct: 449 NKRFQFLGLSLPENLLNDTAVEEYCQKTVTTYWHYHGGCLVGKVVDDNHKVIGIDNLRVV 508

Query: 223 DGSTFSVSPGTNPMATLMMLG 244
           DGSTFSVSPGTNPMATLMMLG
Sbjct: 509 DGSTFSVSPGTNPMATLMMLG 529

[Close]

BLAST of CSPI02G05770 vs. TrEMBL
Match: A0A0A0LJ80_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G055020 PE=3 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 1.4e-57
Identity = 109/116 (93.97%), Postives = 114/116 (98.28%), Query Frame = 1


Query: 1   MKFVHNATDLPTKEEFDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQG 60
           MKFVHNATDLPTKEE+DYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNE+G
Sbjct: 37  MKFVHNATDLPTKEEYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEKG 96

Query: 61  LLKAFDTEDDGKTPFQRFISEDGLENIRGRVLGGGSMVNAGFYSRGHKEFFESAEV 117
           L KAFDTEDDGKTPFQRF+SEDG+ENIRGR+LGGGSMVNAGFYSRGHKEFFESA V
Sbjct: 97  LSKAFDTEDDGKTPFQRFVSEDGVENIRGRILGGGSMVNAGFYSRGHKEFFESAGV 152

[Close]

BLAST of CSPI02G05770 vs. TAIR10
Match: AT1G73050.1 (AT1G73050.1 Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 164.5 bits (415), Expect = 8.4e-41
Identity = 70/131 (53.44%), Postives = 97/131 (74.05%), Query Frame = 1


Query: 113 SAEVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLP 172
           S +V+ NP+VRFNY+S P DL RCV G RK+G++ ++  ++    +   GN+ F F+G P
Sbjct: 409 STDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAP 468

Query: 173 LPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPG 232
           LP +  ND  + ++C++TV+T WHYHGG +VGKVVD + KVI   +LR+VDGSTF++SPG
Sbjct: 469 LPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPG 528

Query: 233 TNPMATLMMLG 244
           TNP ATLMMLG
Sbjct: 529 TNPQATLMMLG 539

[Close]

BLAST of CSPI02G05770 vs. TAIR10
Match: AT1G12570.1 (AT1G12570.1 Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 131.3 bits (329), Expect = 7.9e-31
Identity = 68/152 (44.74%), Postives = 90/152 (59.21%), Query Frame = 1


Query: 101 GFYSRGHKEFFESAEVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNL 160
           G  S GH E  ++   K NPIV FNY+ HPDDL RCV+G++ +  V ++    + K  ++
Sbjct: 408 GPLSTGHLEL-KTRNPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADV 467

Query: 161 KGNKGFMFLGLPLPGNLWNDSA---------VEEYCKKTVATYWHYHGGCLVGKVVDGNY 220
                 + L    P NL    +          EE+C+ TV T WHYHGGC+VG+VVDG+Y
Sbjct: 468 SFEY-LLNLTASTPVNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDY 527

Query: 221 KVIETENLRVVDGSTFSVSPGTNPMATLMMLG 244
           KVI  + LRV+D ST    PGTNP AT+MMLG
Sbjct: 528 KVIGIDRLRVIDMSTVGYCPGTNPQATVMMLG 557

[Close]

BLAST of CSPI02G05770 vs. TAIR10
Match: AT5G51950.1 (AT5G51950.1 Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 131.0 bits (328), Expect = 1.0e-30
Identity = 69/149 (46.31%), Postives = 90/149 (60.40%), Query Frame = 1


Query: 100 AGFYSRGHKEFFESAEVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQN 159
           AG  SRGH E   +     NP VRFNYY  P+DL  CV+G+  +  V  +    K K  +
Sbjct: 419 AGPISRGHLEL-RNTNPDDNPSVRFNYYQEPEDLQICVEGINTIIKVINSKAFSKFKYPD 478

Query: 160 LKGNKGFMFLGLPLPGNL-----WNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVI 219
              + G + L L +P NL      +   + ++C  TV T WHYHGGC VG+VVD NY+V+
Sbjct: 479 ATIH-GLLDLMLSVPTNLRPRHITSMFNLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVL 538

Query: 220 ETENLRVVDGSTFSVSPGTNPMATLMMLG 244
             ++LRV+DGSTF  SPGTNP AT+MMLG
Sbjct: 539 GIDSLRVIDGSTFLKSPGTNPQATVMMLG 565

[Close]

BLAST of CSPI02G05770 vs. TAIR10
Match: AT3G56060.1 (AT3G56060.1 Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 130.2 bits (326), Expect = 1.8e-30
Identity = 70/148 (47.30%), Postives = 89/148 (60.14%), Query Frame = 1


Query: 101 GFYSRGHKEFFESAEVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNL 160
           G  SRGH E   +     NP V FNY+  P+DL +CV+G+  +  V  +    K K   L
Sbjct: 411 GPLSRGHLEL-RNTNPDDNPSVTFNYFKDPEDLNKCVEGLSTIIKVIDSKGYSKYKYP-L 470

Query: 161 KGNKGFMFLGLPLPGNL-----WNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIE 220
              +G + L L LP NL      +   +E+YC  TV T +HYHGGC VGKVVD NYKV+ 
Sbjct: 471 ASARGLLNLILALPTNLRPRHITSTFDLEQYCIDTVMTIYHYHGGCQVGKVVDNNYKVLG 530

Query: 221 TENLRVVDGSTFSVSPGTNPMATLMMLG 244
            + LR++DGSTF  SPGTNP AT+MMLG
Sbjct: 531 VDALRIIDGSTFLKSPGTNPQATIMMLG 556

[Close]

BLAST of CSPI02G05770 vs. TAIR10
Match: AT5G51930.1 (AT5G51930.1 Glucose-methanol-choline (GMC) oxidoreductase family protein)

HSP 1 Score: 129.4 bits (324), Expect = 3.0e-30
Identity = 63/144 (43.75%), Postives = 89/144 (61.81%), Query Frame = 1


Query: 104 SRGHKEFFESAEVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKGN 163
           S+GH +   +   + NP V FNYY  P+DL +CVKG+  +  +  +    K K   +   
Sbjct: 439 SKGHMKL-RNTNPRDNPSVTFNYYQEPEDLNKCVKGLNTIIRMINSKAFSKYKYPGVTAR 498

Query: 164 KGF-MFLGLPL---PGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENL 223
           +   + L LP+   P ++ +   ++++C  TV + WHYHGGC VGKVVD NYKV+  + L
Sbjct: 499 ELLNLMLALPINLRPRHVTSAFNLKQFCIDTVTSVWHYHGGCQVGKVVDKNYKVLGIDGL 558

Query: 224 RVVDGSTFSVSPGTNPMATLMMLG 244
           RV+DGSTF  SPGTNP AT+MMLG
Sbjct: 559 RVIDGSTFLKSPGTNPQATVMMLG 581

[Close]

BLAST of CSPI02G05770 vs. NCBI nr
Match: gi|778665376|ref|XP_011648549.1| (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis sativus])

HSP 1 Score: 278.1 bits (710), Expect = 1.5e-71
Identity = 129/141 (91.49%), Postives = 135/141 (95.74%), Query Frame = 1


Query: 103 YSRGHKEFFESAEVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKG 162
           +S+G  +   S +VKK+PIV+FNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKG
Sbjct: 390 HSKGSLKLNSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKG 449

Query: 163 NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVV 222
           NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVV
Sbjct: 450 NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVV 509

Query: 223 DGSTFSVSPGTNPMATLMMLG 244
           DGSTFSVSPGTNPMATLMMLG
Sbjct: 510 DGSTFSVSPGTNPMATLMMLG 530

[Close]

BLAST of CSPI02G05770 vs. NCBI nr
Match: gi|700211433|gb|KGN66529.1| (hypothetical protein Csa_1G627390 [Cucumis sativus])

HSP 1 Score: 278.1 bits (710), Expect = 1.5e-71
Identity = 129/141 (91.49%), Postives = 135/141 (95.74%), Query Frame = 1


Query: 103 YSRGHKEFFESAEVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKG 162
           +S+G  +   S +VKK+PIV+FNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKG
Sbjct: 390 HSKGSLKLNSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKG 449

Query: 163 NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVV 222
           NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVV
Sbjct: 450 NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVV 509

Query: 223 DGSTFSVSPGTNPMATLMMLG 244
           DGSTFSVSPGTNPMATLMMLG
Sbjct: 510 DGSTFSVSPGTNPMATLMMLG 530

[Close]

BLAST of CSPI02G05770 vs. NCBI nr
Match: gi|778674055|ref|XP_011650118.1| (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis sativus])

HSP 1 Score: 271.2 bits (692), Expect = 1.8e-69
Identity = 124/141 (87.94%), Postives = 134/141 (95.04%), Query Frame = 1


Query: 103 YSRGHKEFFESAEVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKG 162
           +S+G  +   S +VKK+PIV+FNYYSHPDDLARCVKGVRKLGDVFKTPT+EKIKTQ+LKG
Sbjct: 258 HSKGSLKLNSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLKG 317

Query: 163 NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVV 222
           NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVI TENLRVV
Sbjct: 318 NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIGTENLRVV 377

Query: 223 DGSTFSVSPGTNPMATLMMLG 244
           DGSTFS+SPGTNPMAT+MMLG
Sbjct: 378 DGSTFSISPGTNPMATVMMLG 398

[Close]

BLAST of CSPI02G05770 vs. NCBI nr
Match: gi|700205994|gb|KGN61113.1| (hypothetical protein Csa_2G055010 [Cucumis sativus])

HSP 1 Score: 271.2 bits (692), Expect = 1.8e-69
Identity = 124/141 (87.94%), Postives = 134/141 (95.04%), Query Frame = 1


Query: 103 YSRGHKEFFESAEVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKG 162
           +S+G  +   S +VKK+PIV+FNYYSHPDDLARCVKGVRKLGDVFKTPT+EKIKTQ+LKG
Sbjct: 74  HSKGSLKLNSSIDVKKSPIVQFNYYSHPDDLARCVKGVRKLGDVFKTPTMEKIKTQDLKG 133

Query: 163 NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVV 222
           NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVI TENLRVV
Sbjct: 134 NKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIGTENLRVV 193

Query: 223 DGSTFSVSPGTNPMATLMMLG 244
           DGSTFS+SPGTNPMAT+MMLG
Sbjct: 194 DGSTFSISPGTNPMATVMMLG 214

[Close]

BLAST of CSPI02G05770 vs. NCBI nr
Match: gi|659069829|ref|XP_008451990.1| (PREDICTED: (R)-mandelonitrile lyase 1-like isoform X1 [Cucumis melo])

HSP 1 Score: 256.9 bits (655), Expect = 3.5e-65
Identity = 120/140 (85.71%), Postives = 128/140 (91.43%), Query Frame = 1


Query: 104 SRGHKEFFESAEVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKGN 163
           S+G  +   S +VKK+PIV+FNYYSHP DLARCVK VRKLGDVFKTPT+EKIKTQ+L+GN
Sbjct: 391 SKGSLKLNSSIDVKKSPIVQFNYYSHPGDLARCVKSVRKLGDVFKTPTMEKIKTQDLEGN 450

Query: 164 KGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVVD 223
           K FMFLGLPLP NLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVI  ENLRVVD
Sbjct: 451 KRFMFLGLPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIGIENLRVVD 510

Query: 224 GSTFSVSPGTNPMATLMMLG 244
           GSTFSVSPGTNPMATLMMLG
Sbjct: 511 GSTFSVSPGTNPMATLMMLG 530

The following BLAST results are available for this feature:

BLAST of CSPI02G05770 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MDLL_ARATH	1.5e-39	53.44	(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana GN=At1g73050 PE=2 SV=1	[more]
MDL1_PRUSE	1.8e-37	47.92	(R)-mandelonitrile lyase 1 OS=Prunus serotina GN=MDL1 PE=1 SV=1	[more]
MDL3_PRUSE	3.4e-36	48.95	(R)-mandelonitrile lyase 3 OS=Prunus serotina GN=MDL3 PE=2 SV=1	[more]
MDL2_PRUDU	4.5e-36	47.22	(R)-mandelonitrile lyase 2 OS=Prunus dulcis GN=MDL2 PE=1 SV=1	[more]
MDL1_PRUDU	7.2e-34	46.15	(R)-mandelonitrile lyase 1 OS=Prunus dulcis GN=MDL1 PE=1 SV=1	[more]

BLAST of CSPI02G05770 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LZU8_CUCSA	1.0e-71	91.49	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G627390 PE=3 SV=1	[more]
A0A0A0LH79_CUCSA	1.3e-69	87.94	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G055010 PE=4 SV=1	[more]
A0A0A0LK76_CUCSA	2.2e-61	79.43	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G055000 PE=3 SV=1	[more]
A0A0A0LK79_CUCSA	5.9e-59	77.30	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G055050 PE=3 SV=1	[more]
A0A0A0LJ80_CUCSA	1.4e-57	93.97	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G055020 PE=3 SV=1	[more]

BLAST of CSPI02G05770 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G73050.1	8.4e-41	53.44	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G12570.1	7.9e-31	44.74	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51950.1	1.0e-30	46.31	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT3G56060.1	1.8e-30	47.30	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51930.1	3.0e-30	43.75	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]

BLAST of CSPI02G05770 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778665376\|ref\|XP_011648549.1\|	1.5e-71	91.49	PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis sativus]	[more]
gi\|700211433\|gb\|KGN66529.1\|	1.5e-71	91.49	hypothetical protein Csa_1G627390 [Cucumis sativus]	[more]
gi\|778674055\|ref\|XP_011650118.1\|	1.8e-69	87.94	PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis sativus]	[more]
gi\|700205994\|gb\|KGN61113.1\|	1.8e-69	87.94	hypothetical protein Csa_2G055010 [Cucumis sativus]	[more]
gi\|659069829\|ref\|XP_008451990.1\|	3.5e-65	85.71	PREDICTED: (R)-mandelonitrile lyase 1-like isoform X1 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000172	GMC_OxRdtase_N
IPR007867	GMC_OxRtase_C
IPR023753	FAD/NAD-binding_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0016614	oxidoreductase activity, acting on CH-OH group of donors
GO:0050660	flavin adenine dinucleotide binding
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016614	oxidoreductase activity, acting on CH-OH group of donors
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0016829	lyase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI02G05770.1	CSPI02G05770.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PFAM	PF00732	GMC_oxred_N	coord: 16..117 score: 4.1
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PROSITE	PS00623	GMC_OXRED_1	coord: 89..112 scor
IPR007867	Glucose-methanol-choline oxidoreductase, C-terminal	PFAM	PF05199	GMC_oxred_C	coord: 113..243 score: 9.2
IPR023753	FAD/NAD(P)-binding domain	GENE3D	G3DSA:3.50.50.60		coord: 176..243 score: 1.6E-19coord: 119..131 score: 1.6E-19coord: 10..113 score: 8.0
IPR023753	FAD/NAD(P)-binding domain	unknown	SSF51905	FAD/NAD(P)-binding domain	coord: 12..243 score: 3.19
None	No IPR available	PANTHER	PTHR11552	GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE	coord: 113..243 score: 3.4
None	No IPR available	PANTHER	PTHR11552:SF82	(R)-MANDELONITRILE LYASE-LIKE	coord: 113..243 score: 3.4
None	No IPR available	PROFILE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..28 score:

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI02G05770	CsaV3_2G008120	Cucumber (Chinese Long) v3	cpicucB080
CSPI02G05770	Cla97C11G218200	Watermelon (97103) v2	cpiwmbB111
CSPI02G05770	gCucsa.089570.2	Cucumber (Gy14) v1	cgycpiB097
CSPI02G05770	CmoCh05G007130	Cucurbita moschata (Rifu)	cmocpiB784
CSPI02G05770	CsGy2G005920	Cucumber (Gy14) v2	cgybcpiB056

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI02G05770	Cucumber (Chinese Long) v3	cpicucB077
CSPI02G05770	Watermelon (97103) v2	cpiwmbB152
CSPI02G05770	Wax gourd	cpiwgoB138
CSPI02G05770	Wax gourd	cpiwgoB212
CSPI02G05770	Wild cucumber (PI 183967)	cpicpiB012
CSPI02G05770	Cucumber (Gy14) v1	cgycpiB099
CSPI02G05770	Cucurbita maxima (Rimu)	cmacpiB071
CSPI02G05770	Cucurbita maxima (Rimu)	cmacpiB074
CSPI02G05770	Cucurbita maxima (Rimu)	cmacpiB114
CSPI02G05770	Cucurbita maxima (Rimu)	cmacpiB795
CSPI02G05770	Cucurbita moschata (Rifu)	cmocpiB065
CSPI02G05770	Cucurbita moschata (Rifu)	cmocpiB107
CSPI02G05770	Cucumber (Chinese Long) v2	cpicuB064
CSPI02G05770	Cucumber (Chinese Long) v2	cpicuB065
CSPI02G05770	Melon (DHL92) v3.5.1	cpimeB115
CSPI02G05770	Melon (DHL92) v3.5.1	cpimeB139
CSPI02G05770	Watermelon (Charleston Gray)	cpiwcgB110
CSPI02G05770	Watermelon (97103) v1	cpiwmB166
CSPI02G05770	Watermelon (97103) v1	cpiwmB177
CSPI02G05770	Cucurbita pepo (Zucchini)	cpecpiB095
CSPI02G05770	Cucurbita pepo (Zucchini)	cpecpiB373
CSPI02G05770	Bottle gourd (USVL1VR-Ls)	cpilsiB096
CSPI02G05770	Bottle gourd (USVL1VR-Ls)	cpilsiB135
CSPI02G05770	Melon (DHL92) v3.6.1	cpimedB111
CSPI02G05770	Melon (DHL92) v3.6.1	cpimedB132
CSPI02G05770	Silver-seed gourd	carcpiB0064
CSPI02G05770	Silver-seed gourd	carcpiB0824