CSPI02G04780 (gene) Wild cucumber (PI 183967)

NameCSPI02G04780
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionPectinesterase
LocationChr2 : 3434664 .. 3436206 (-)
   



The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGGTACAACCTCCATTTCTCTTGCTCTTCACTCTGTTCAATGCTGTTGTCTTTGGTTTTGAAGCTGTGAATCAGGCAAGAGACTTTGTCCTTACGGTTCAATCCATTGTTATTGTTGACAAGTCGGGAAATGGCAATTTCCAGACCGTTCAAGCAGCCATTGACTCAGTTCCACCTAATAACAATCATTGGATTAAGATTCAAATAAACCCTGGTGTATACAAGTAATACTAATACAGTACACATTATGAAATTTGGTATTGAGGTTTTGTTCTAATATTGCTGGCAAACCTTTTGCAGAGAAAAGGTGACAATCCCACTAGAAAAGCCTTTCATATATCTCGAAGGAGCTGACAGCAGTAATACGGTTATCACGTTCGATGACCATCAACAAACAGACACTAGTGCTACATTTACTTCACGTCCTCCCAACATTATTGTTAGAGGCATTACTTTTGAGGTGCTGTGGCTTCTAAAAACTGATTTTATATTCATAGCATTATTTGAGATTTTGAAGCTATGTAAGGTGCAAGTTAAGTTTGTAGTTAATGATATATATCGTACGGCGTACGCACCTGAGATAGGTTTGAGCAGGATTCTATAGCTCAATCATTGATCAATGTCTGTCTTGTTACAGAATTAATTGTTGCTTTTATATGGTAAATCAACATGCTTGATTATGTGTTATTGTGATGCACACAGAACTCATTCAACCTTCGAGAAGCTCCAGAGCTGTTTTCTTGCGATGATGGAACTTACATTACACAAGCAATAGCAGCAAGAATCTACGGCGACAAATCAGCTTTTTTCAACTGTGGATTCAAAGGGTATCAAGACACGTTATGGGATGTACAAGGTCGCCATTTCTTCTCACATTGCTACATTGAAGGAGCCATAGACTTCATCTTTGGTAGTGGCCAATCAGTTTATGAGGTACTCAGTTCAAATGTCAATATTCTGTCATTTACCCCATTGAAAGAGACTTACTTTTTAGCAACTTGGGACCATAAATGCATGTTTTTTTGACAATGTAGGATTGCATGATAAATGTTAATGTTGCAAGTCTTCCTCAAGTCTACCAAGGGTACATTACTGCACAAAGTCGACAATCAGCAGCAGATCCAAGTGGATTTGTGTTTAAAGAGTGCACCATAAAGGGTAGTGGCAAAGCTTTGTTGGGGAGAGCATATGGTCCTTTTTCCAGAGTTATATTCAAAGATGCTATAATGGGTTCAGTGGTAGCACCAGAGGGATGGTACGCCTGGCACTTCAAGGGCAAAGAGTAAGTTCAGGGCCAGAGTTCTTTCAAGCTGCACAGTGAAGCATAATAGTCATTTAGGACTTATTGAATTGCCAAGTTCTAACTGGTGTTACATTCTTTAGGGAAAATTTTATGTACGTTGAGGAGAATTGTACCGGACCAGGAGCTTCCACTTCCATGAGAGTTCCATGGGCAAAGACACTTGATGCCTCGCATCTAACAGGCTTCTCCGTGGAATCCTTCATTAACCAAGATGGGTGGATTCCCACAGTCCTTTGA

mRNA sequence

ATGCAGGTACAACCTCCATTTCTCTTGCTCTTCACTCTGTTCAATGCTGTTGTCTTTGGTTTTGAAGCTGTGAATCAGGCAAGAGACTTTGTCCTTACGGTTCAATCCATTGTTATTGTTGACAAGTCGGGAAATGGCAATTTCCAGACCGTTCAAGCAGCCATTGACTCAGTTCCACCTAATAACAATCATTGGATTAAGATTCAAATAAACCCTGGTGTATACAAAGAAAAGGTGACAATCCCACTAGAAAAGCCTTTCATATATCTCGAAGGAGCTGACAGCAGTAATACGGTTATCACGTTCGATGACCATCAACAAACAGACACTAGTGCTACATTTACTTCACGTCCTCCCAACATTATTGTTAGAGGCATTACTTTTGAGAACTCATTCAACCTTCGAGAAGCTCCAGAGCTGTTTTCTTGCGATGATGGAACTTACATTACACAAGCAATAGCAGCAAGAATCTACGGCGACAAATCAGCTTTTTTCAACTGTGGATTCAAAGGGTATCAAGACACGTTATGGGATGTACAAGGTCGCCATTTCTTCTCACATTGCTACATTGAAGGAGCCATAGACTTCATCTTTGGTAGTGGCCAATCAGTTTATGAGGATTGCATGATAAATGTTAATGTTGCAAGTCTTCCTCAAGTCTACCAAGGGTACATTACTGCACAAAGTCGACAATCAGCAGCAGATCCAAGTGGATTTGTGTTTAAAGAGTGCACCATAAAGGGTAGTGGCAAAGCTTTGTTGGGGAGAGCATATGGTCCTTTTTCCAGAGTTATATTCAAAGATGCTATAATGGGTTCAGTGGTAGCACCAGAGGGATGGTACGCCTGGCACTTCAAGGGCAAAGAGGAAAATTTTATGTACGTTGAGGAGAATTGTACCGGACCAGGAGCTTCCACTTCCATGAGAGTTCCATGGGCAAAGACACTTGATGCCTCGCATCTAACAGGCTTCTCCGTGGAATCCTTCATTAACCAAGATGGGTGGATTCCCACAGTCCTTTGA

Coding sequence (CDS)

ATGCAGGTACAACCTCCATTTCTCTTGCTCTTCACTCTGTTCAATGCTGTTGTCTTTGGTTTTGAAGCTGTGAATCAGGCAAGAGACTTTGTCCTTACGGTTCAATCCATTGTTATTGTTGACAAGTCGGGAAATGGCAATTTCCAGACCGTTCAAGCAGCCATTGACTCAGTTCCACCTAATAACAATCATTGGATTAAGATTCAAATAAACCCTGGTGTATACAAAGAAAAGGTGACAATCCCACTAGAAAAGCCTTTCATATATCTCGAAGGAGCTGACAGCAGTAATACGGTTATCACGTTCGATGACCATCAACAAACAGACACTAGTGCTACATTTACTTCACGTCCTCCCAACATTATTGTTAGAGGCATTACTTTTGAGAACTCATTCAACCTTCGAGAAGCTCCAGAGCTGTTTTCTTGCGATGATGGAACTTACATTACACAAGCAATAGCAGCAAGAATCTACGGCGACAAATCAGCTTTTTTCAACTGTGGATTCAAAGGGTATCAAGACACGTTATGGGATGTACAAGGTCGCCATTTCTTCTCACATTGCTACATTGAAGGAGCCATAGACTTCATCTTTGGTAGTGGCCAATCAGTTTATGAGGATTGCATGATAAATGTTAATGTTGCAAGTCTTCCTCAAGTCTACCAAGGGTACATTACTGCACAAAGTCGACAATCAGCAGCAGATCCAAGTGGATTTGTGTTTAAAGAGTGCACCATAAAGGGTAGTGGCAAAGCTTTGTTGGGGAGAGCATATGGTCCTTTTTCCAGAGTTATATTCAAAGATGCTATAATGGGTTCAGTGGTAGCACCAGAGGGATGGTACGCCTGGCACTTCAAGGGCAAAGAGGAAAATTTTATGTACGTTGAGGAGAATTGTACCGGACCAGGAGCTTCCACTTCCATGAGAGTTCCATGGGCAAAGACACTTGATGCCTCGCATCTAACAGGCTTCTCCGTGGAATCCTTCATTAACCAAGATGGGTGGATTCCCACAGTCCTTTGA
BLAST of CSPI02G04780 vs. Swiss-Prot
Match: PME66_ARATH (Probable pectinesterase 66 OS=Arabidopsis thaliana GN=PME66 PE=2 SV=2)

HSP 1 Score: 304.3 bits (778), Expect = 1.7e-81
Identity = 154/330 (46.67%), Postives = 210/330 (63.64%), Query Frame = 1

Query: 8   LLLFTLFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPPNNNHWIK 67
           +++ TLF   V+G+ A  +   + +TV      D +G GNF TVQ+AIDS+ P N++WI+
Sbjct: 14  MVVMTLF---VYGYSAAAEQIAYTITV------DLNGGGNFTTVQSAIDSISPPNHNWIR 73

Query: 68  IQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPNIIVRGIT 127
           +    G+Y+EKVTIP EK FIYL+G     TVI +DDHQ TD SATFT+   +I++ GIT
Sbjct: 74  VFTQNGIYREKVTIPKEKGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGIT 133

Query: 128 FENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSH 187
           F+N++N+         ++   I  A+AAR+ GD+    +  F G QDTL+D +GRH++  
Sbjct: 134 FKNTYNIVP-------NNKREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKR 193

Query: 188 CYIEGAIDFIFGSGQSVYEDCMINVNVASL-PQVYQGYITAQSRQSAADPSGFVFKECTI 247
           C I G IDFIFG GQS++++C +N+ +    P    G ITA  R S +D  GFVF +CT+
Sbjct: 194 CIISGGIDFIFGYGQSLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTV 253

Query: 248 KGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGAST 307
            G GK LLGRA+G  +RVIF  + +  VV P GW AW  KG E +  +VE  CTG GA T
Sbjct: 254 TGVGKTLLGRAWGSNARVIFDRSRLSDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADT 313

Query: 308 SMRVPWAKTLDASHLTGFSVESFINQDGWI 337
           S RVPW K L  S + GF+  SFI+QDGWI
Sbjct: 314 SQRVPWLKKLSLSEVDGFASVSFIDQDGWI 327

BLAST of CSPI02G04780 vs. Swiss-Prot
Match: PME55_ARATH (Probable pectinesterase 55 OS=Arabidopsis thaliana GN=PME55 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 9.2e-80
Identity = 145/299 (48.49%), Postives = 192/299 (64.21%), Query Frame = 1

Query: 38  VIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSN 97
           VIVD+SG+GNF T+Q AIDSVP NN HW  I +  G+Y+EK+TIP +KPFI + GA   +
Sbjct: 29  VIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRS 88

Query: 98  TVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAIAARI 157
           T + +DDH     S TF +   N +V+ ITF NS+N     ++    +      A+AA I
Sbjct: 89  TRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKI----NKNPRVPAVAAFI 148

Query: 158 YGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASL 217
            GDKSAF++ GF G QDTLWD  GRH+F  C I+GA+DFI GSGQS+Y+ C+I V    L
Sbjct: 149 GGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQL 208

Query: 218 PQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAP 277
                GYITAQ R +A D +GFVF  C + G GKA LGRA+ P+SRVIF ++ +  VV P
Sbjct: 209 GPGVTGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDP 268

Query: 278 EGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWI 337
            GW+ W+++G E+   Y E  C G G++TS R  W K L AS +   +  SFIN+ GW+
Sbjct: 269 LGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWV 323

BLAST of CSPI02G04780 vs. Swiss-Prot
Match: PME29_ARATH (Probable pectinesterase 29 OS=Arabidopsis thaliana GN=PME29 PE=2 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.0e-78
Identity = 143/302 (47.35%), Postives = 188/302 (62.25%), Query Frame = 1

Query: 35  QSIVIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGAD 94
           Q  V VD+SG+GNF T+Q AIDSVP NN HW  I +  G+Y+EK+ IP EKPFI L GA 
Sbjct: 31  QQQVFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAG 90

Query: 95  SSNTVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAIA 154
              T + +DDH     S TF++   N +V+ ITF NS+N     ++    +    T A+A
Sbjct: 91  KRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKM----NKNPRTPAVA 150

Query: 155 ARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNV 214
           A I GDKSAF++ GF G QDTLWD  GRH+F  C I+GA+DFIFG+GQS+Y+ C+I V  
Sbjct: 151 ALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLG 210

Query: 215 ASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSV 274
             L     GYITAQ R +  D +GF+F  C + G+G A LGR +  +SRVIF ++ +  V
Sbjct: 211 GQLEPGLAGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDV 270

Query: 275 VAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDG 334
           V PEGW AW+F G E   ++ E  C G GA+   RV W K L  S +   +  SFIN+ G
Sbjct: 271 VVPEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGG 328

Query: 335 WI 337
           W+
Sbjct: 331 WV 328

BLAST of CSPI02G04780 vs. Swiss-Prot
Match: PME10_ARATH (Putative pectinesterase 10 OS=Arabidopsis thaliana GN=PME10 PE=2 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 6.2e-76
Identity = 141/305 (46.23%), Postives = 192/305 (62.95%), Query Frame = 1

Query: 34  VQSIVIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGA 93
           +   +IV+ +    F+TVQ+AIDS+P  N  WI+I I+ G+Y EKVTIP  K +IY++G 
Sbjct: 37  IAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQGG 96

Query: 94  DSSNTVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAI 153
               T+I + DHQ T+TSATFTS P NII+ GITF+N +N+  +         +    A+
Sbjct: 97  GIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS--------SSPTKPAV 156

Query: 154 AARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVN 213
           AA + GDK A  +  F G+QDTL+D  GRH++  C I G IDFIFG  QS++E C + + 
Sbjct: 157 AAMMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLR 216

Query: 214 VASLP--QVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIM 273
           V   P  +VY G ITAQ R S  D  GFVFK+CT+ GSGKALLGRA+  +SRVIF  ++ 
Sbjct: 217 VGIYPPNEVY-GTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMF 276

Query: 274 GSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFIN 333
              + P GW AW  KG+E +  +VE  CTG GA TS RVPW        +  F+  +FI+
Sbjct: 277 SDNILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFID 332

Query: 334 QDGWI 337
           ++GW+
Sbjct: 337 EEGWL 332

BLAST of CSPI02G04780 vs. Swiss-Prot
Match: PME52_ARATH (Putative pectinesterase 52 OS=Arabidopsis thaliana GN=PME52 PE=2 SV=2)

HSP 1 Score: 258.1 bits (658), Expect = 1.4e-67
Identity = 128/245 (52.24%), Postives = 161/245 (65.71%), Query Frame = 1

Query: 98  TVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTY-ITQAIAAR 157
           T IT++ H  TD S+TFTS P +I+VR ++  N++N      L   +  ++ I  A+A  
Sbjct: 48  TTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYN--RLTSLTKANGMSWDIKPAVAIS 107

Query: 158 IYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVAS 217
           +YGDKSAF+NC F G QDT+WD  GRH F +CYIEGAIDFIFGSGQSVYEDC IN    +
Sbjct: 108 VYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINATAGA 167

Query: 218 LP-QVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVV 277
           L  +V  GYITAQ R S +DPSGFVF   ++ GS    LGRAYGPFSRVIF    + SVV
Sbjct: 168 LASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDLSSVV 227

Query: 278 APEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHL-TGFSVESFINQDG 337
            PEGWY+WH+ G E +F Y E  C G G+  S RVPW   L + +    FS+ +FI+QD 
Sbjct: 228 HPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFIDQDQ 287

Query: 338 WIPTV 340
           WI  +
Sbjct: 288 WISNI 290

BLAST of CSPI02G04780 vs. TrEMBL
Match: A0A0A0LH00_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_2G034610 PE=4 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 3.6e-200
Identity = 338/340 (99.41%), Postives = 340/340 (100.00%), Query Frame = 1

Query: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPP 60
           MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLT+QS+VIVDKSGNGNFQTVQAAIDSVPP
Sbjct: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120
           NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN
Sbjct: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120

Query: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180
           IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ
Sbjct: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180

Query: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240
           GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV
Sbjct: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240

Query: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300
           FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT
Sbjct: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300

Query: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL 341
           GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL
Sbjct: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL 340

BLAST of CSPI02G04780 vs. TrEMBL
Match: A0A061F313_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_026775 PE=4 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 8.1e-99
Identity = 186/331 (56.19%), Postives = 226/331 (68.28%), Query Frame = 1

Query: 8   LLLFTLFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPPNNNHWIK 67
           LL+ +L  ++     A N    F  T+    +VDKSG GNF ++Q+AIDS+P NN  WIK
Sbjct: 11  LLILSLRTSMADDCSAGNGGSQFASTI----VVDKSGKGNFNSIQSAIDSIPSNNAQWIK 70

Query: 68  IQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPNIIVRGIT 127
           ++INPGVY EKV IP++KP I LEG D S T ITF  H +TD S TFTS   NI+ +GIT
Sbjct: 71  VRINPGVYTEKVVIPIDKPCIVLEGQDRSVTTITFYAHDRTDRSCTFTSFADNIVAKGIT 130

Query: 128 FENSFNLREAPELFSCDDGTY-ITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFS 187
           F+NS+N     E  S +     ++QA+AARI GDKSAFF CGF G QDTLWD  GRH+F 
Sbjct: 131 FKNSYNHLLLLERLSSERPIPGVSQAVAARILGDKSAFFQCGFLGLQDTLWDAIGRHYFY 190

Query: 188 HCYIEGAIDFIFGSGQSVYEDCMINVNVASLP-QVYQGYITAQSRQSAADPSGFVFKECT 247
            C+IEGA+DFIFG GQS+YEDC INV   +   Q+  GYITAQ RQS+ DPSGFVFK   
Sbjct: 191 QCHIEGAVDFIFGYGQSLYEDCSINVTAGAFSSQIPLGYITAQGRQSSNDPSGFVFKRGE 250

Query: 248 IKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGAS 307
           I G+ +  LGRAYGP+SRVIF++  M + V  EGW AW + GKEENFMY E NC G G+ 
Sbjct: 251 IFGNTQPYLGRAYGPYSRVIFQETTMNAEVVSEGWDAWRYPGKEENFMYAEVNCQGAGSD 310

Query: 308 TSMRVPWAKTLDASHLTGFSVESFINQDGWI 337
           TS RVPW K L+ S L  FS  SFI+ DGWI
Sbjct: 311 TSRRVPWEKKLNPSQLNQFSRSSFIDNDGWI 337

BLAST of CSPI02G04780 vs. TrEMBL
Match: V4T5U1_9ROSI (Pectinesterase (Fragment) OS=Citrus clementina GN=CICLE_v10003837mg PE=4 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 3.8e-96
Identity = 181/309 (58.58%), Postives = 215/309 (69.58%), Query Frame = 1

Query: 38  VIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSN 97
           V VD+S +  ++TVQ+AIDS+P  N+ WIKI I  G YKEK+ I   KP I LEG  S  
Sbjct: 42  VTVDQSSS-QYRTVQSAIDSIPAENSQWIKIHIKAGTYKEKIKIQRNKPCILLEGEGSGV 101

Query: 98  TVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNL---------REAPELFSCDDGTY 157
           T IT+DDHQ TDTSATF+S   N++ +GITFENS+NL         R   +L +   G  
Sbjct: 102 TKITYDDHQSTDTSATFSSFADNVVAKGITFENSYNLAVEILAELDRRGRDLGTLQAGND 161

Query: 158 ITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDC 217
           ITQA+AARIYGDKSAF++C F G QDTLWDVQGRHFF  CYIEGAIDFIFG+GQSVYEDC
Sbjct: 162 ITQALAARIYGDKSAFYDCRFLGVQDTLWDVQGRHFFKSCYIEGAIDFIFGNGQSVYEDC 221

Query: 218 MINVNVASLPQVY-QGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFK 277
            +N     L      GYITAQSR SA DP GFVF+   + G+G+A LGRAYGP+SRVIF 
Sbjct: 222 SVNSTAGLLIGGRGSGYITAQSRGSANDPGGFVFRGGEVTGTGQAYLGRAYGPYSRVIFY 281

Query: 278 DAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVE 337
           +A + SVV P GW AW+ +G+E NFMY E NC GPG+ TS RV W K +D   L  +S  
Sbjct: 282 NAWLSSVVTPPGWNAWNLQGQEGNFMYAEVNCKGPGSDTSNRVSWEKKIDPKLLYKYSTS 341

BLAST of CSPI02G04780 vs. TrEMBL
Match: B9STE8_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_1318720 PE=4 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 1.4e-95
Identity = 176/344 (51.16%), Postives = 242/344 (70.35%), Query Frame = 1

Query: 3   VQPPFLLLFTL-------FNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAI 62
           +Q P+LL+F +       + AVV+G  AV + +++   V S V V KSG+  F+T+Q AI
Sbjct: 1   MQLPWLLVFIIIIMWLNVYLAVVYG--AV-ECKNWSENVASTVTVGKSGHEQFKTIQTAI 60

Query: 63  DSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFT 122
           DS+P +NN WIKI ++PGVY EKV IP EKP I+LEG+  S + I F+ H++TDTSATF+
Sbjct: 61  DSIPQSNNKWIKITVSPGVYMEKVNIPEEKPCIFLEGSGRSLSTIVFNAHEETDTSATFS 120

Query: 123 SRPPNIIVRGITFENSFN--LREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQ 182
           S   N +  GITF+NS+N  L+E  E         I QA+AA+++GDKSAF+ CGF G+Q
Sbjct: 121 SLADNFLATGITFQNSYNRALKEEDEK--------IRQAVAAKLFGDKSAFYECGFVGFQ 180

Query: 183 DTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLP-QVYQGYITAQSRQS 242
           DTLWD +GRH+F +CYIEGAIDFIFG+GQS Y+DC++N    ++   V  GYITAQSR S
Sbjct: 181 DTLWDEKGRHYFYNCYIEGAIDFIFGNGQSFYQDCLLNATSPAVAGNVEAGYITAQSRGS 240

Query: 243 AADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENF 302
             + +GFVF++ ++ GS +  LGRAYGP+SRVIF +    ++V+P+GW AWHF+G++ N 
Sbjct: 241 NTETTGFVFRKGSVSGSSQTYLGRAYGPYSRVIFHETTFNAIVSPQGWNAWHFQGRQGNL 300

Query: 303 MYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWI 337
           +Y E +C GPG+ TS RVPW K LD   +  FS  SFI++DGW+
Sbjct: 301 VYTEIDCKGPGSDTSKRVPWMKKLDQEEICKFSRSSFIDEDGWL 333

BLAST of CSPI02G04780 vs. TrEMBL
Match: A0A067GSJ9_CITSI (Pectinesterase OS=Citrus sinensis GN=CISIN_1g038332mg PE=4 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 5.4e-95
Identity = 186/331 (56.19%), Postives = 223/331 (67.37%), Query Frame = 1

Query: 8   LLLFTLFNAVVFGFEAVNQARDFV-LTVQSIVIVDKSGNGNFQTVQAAIDSVPPNNNHWI 67
           L++FTL + +   F A    +D     V   V VD+S +  ++TVQ+AIDS+P  NN WI
Sbjct: 8   LIVFTLLSTI--SFRAARADQDCKGSNVAYTVTVDQSSS-QYRTVQSAIDSIPAENNQWI 67

Query: 68  KIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPNIIVRGI 127
           K+ I  G YKEKV I   KP I LEG  S  T IT+DDHQ TDTSATF+S   N++ +GI
Sbjct: 68  KVHIKAGTYKEKVKIQRNKPCILLEGEGSGVTKITYDDHQSTDTSATFSSFADNVVAKGI 127

Query: 128 TFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFS 187
           TFE      E  +L +   G  ITQA+AARIYGDKSAF++C F G QDTLWDVQGRHFF 
Sbjct: 128 TFEV-----EGRDLGTLQAGNDITQALAARIYGDKSAFYDCRFLGVQDTLWDVQGRHFFK 187

Query: 188 HCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVY-QGYITAQSRQSAADPSGFVFKECT 247
            CYIEGAIDFIFG+GQSVYEDC +N     L      GYITAQSR SA DP GFVF+   
Sbjct: 188 SCYIEGAIDFIFGNGQSVYEDCSVNSTAGLLIGGRGSGYITAQSRGSANDPGGFVFRGGE 247

Query: 248 IKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGAS 307
           + G+G+A LGRAYGP+SRVIF +A + SVV P GW AW+ +G+E NFMY E NC GPG+ 
Sbjct: 248 VTGTGQAYLGRAYGPYSRVIFYNAWLSSVVTPPGWNAWNLQGQEGNFMYAEVNCKGPGSD 307

Query: 308 TSMRVPWAKTLDASHLTGFSVESFINQDGWI 337
           TS RV W K +D   L  +S   FINQDGWI
Sbjct: 308 TSNRVSWEKKIDPKLLYKYSTSYFINQDGWI 330

BLAST of CSPI02G04780 vs. TAIR10
Match: AT2G47280.1 (AT2G47280.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 304.3 bits (778), Expect = 9.5e-83
Identity = 154/330 (46.67%), Postives = 210/330 (63.64%), Query Frame = 1

Query: 8   LLLFTLFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPPNNNHWIK 67
           +++ TLF   V+G+ A  +   + +TV      D +G GNF TVQ+AIDS+ P N++WI+
Sbjct: 14  MVVMTLF---VYGYSAAAEQIAYTITV------DLNGGGNFTTVQSAIDSISPPNHNWIR 73

Query: 68  IQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPNIIVRGIT 127
           +    G+Y+EKVTIP EK FIYL+G     TVI +DDHQ TD SATFT+   +I++ GIT
Sbjct: 74  VFTQNGIYREKVTIPKEKGFIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGIT 133

Query: 128 FENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSH 187
           F+N++N+         ++   I  A+AAR+ GD+    +  F G QDTL+D +GRH++  
Sbjct: 134 FKNTYNIVP-------NNKREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKR 193

Query: 188 CYIEGAIDFIFGSGQSVYEDCMINVNVASL-PQVYQGYITAQSRQSAADPSGFVFKECTI 247
           C I G IDFIFG GQS++++C +N+ +    P    G ITA  R S +D  GFVF +CT+
Sbjct: 194 CIISGGIDFIFGYGQSLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTV 253

Query: 248 KGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGAST 307
            G GK LLGRA+G  +RVIF  + +  VV P GW AW  KG E +  +VE  CTG GA T
Sbjct: 254 TGVGKTLLGRAWGSNARVIFDRSRLSDVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADT 313

Query: 308 SMRVPWAKTLDASHLTGFSVESFINQDGWI 337
           S RVPW K L  S + GF+  SFI+QDGWI
Sbjct: 314 SQRVPWLKKLSLSEVDGFASVSFIDQDGWI 327

BLAST of CSPI02G04780 vs. TAIR10
Match: AT5G18990.1 (AT5G18990.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 298.5 bits (763), Expect = 5.2e-81
Identity = 145/299 (48.49%), Postives = 192/299 (64.21%), Query Frame = 1

Query: 38  VIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSN 97
           VIVD+SG+GNF T+Q AIDSVP NN HW  I +  G+Y+EK+TIP +KPFI + GA   +
Sbjct: 29  VIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRS 88

Query: 98  TVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAIAARI 157
           T + +DDH     S TF +   N +V+ ITF NS+N     ++    +      A+AA I
Sbjct: 89  TRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKI----NKNPRVPAVAAFI 148

Query: 158 YGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASL 217
            GDKSAF++ GF G QDTLWD  GRH+F  C I+GA+DFI GSGQS+Y+ C+I V    L
Sbjct: 149 GGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQL 208

Query: 218 PQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAP 277
                GYITAQ R +A D +GFVF  C + G GKA LGRA+ P+SRVIF ++ +  VV P
Sbjct: 209 GPGVTGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDP 268

Query: 278 EGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWI 337
            GW+ W+++G E+   Y E  C G G++TS R  W K L AS +   +  SFIN+ GW+
Sbjct: 269 LGWWEWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWV 323

BLAST of CSPI02G04780 vs. TAIR10
Match: AT3G24130.1 (AT3G24130.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 295.0 bits (754), Expect = 5.8e-80
Identity = 143/302 (47.35%), Postives = 188/302 (62.25%), Query Frame = 1

Query: 35  QSIVIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGAD 94
           Q  V VD+SG+GNF T+Q AIDSVP NN HW  I +  G+Y+EK+ IP EKPFI L GA 
Sbjct: 31  QQQVFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAG 90

Query: 95  SSNTVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAIA 154
              T + +DDH     S TF++   N +V+ ITF NS+N     ++    +    T A+A
Sbjct: 91  KRLTRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKM----NKNPRTPAVA 150

Query: 155 ARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNV 214
           A I GDKSAF++ GF G QDTLWD  GRH+F  C I+GA+DFIFG+GQS+Y+ C+I V  
Sbjct: 151 ALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLG 210

Query: 215 ASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSV 274
             L     GYITAQ R +  D +GF+F  C + G+G A LGR +  +SRVIF ++ +  V
Sbjct: 211 GQLEPGLAGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDV 270

Query: 275 VAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDG 334
           V PEGW AW+F G E   ++ E  C G GA+   RV W K L  S +   +  SFIN+ G
Sbjct: 271 VVPEGWDAWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGG 328

Query: 335 WI 337
           W+
Sbjct: 331 WV 328

BLAST of CSPI02G04780 vs. TAIR10
Match: AT2G19150.1 (AT2G19150.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 285.8 bits (730), Expect = 3.5e-77
Identity = 141/305 (46.23%), Postives = 192/305 (62.95%), Query Frame = 1

Query: 34  VQSIVIVDKSGNGNFQTVQAAIDSVPPNNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGA 93
           +   +IV+ +    F+TVQ+AIDS+P  N  WI+I I+ G+Y EKVTIP  K +IY++G 
Sbjct: 37  IAKTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQGG 96

Query: 94  DSSNTVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTYITQAI 153
               T+I + DHQ T+TSATFTS P NII+ GITF+N +N+  +         +    A+
Sbjct: 97  GIEKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIASS--------SSPTKPAV 156

Query: 154 AARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVN 213
           AA + GDK A  +  F G+QDTL+D  GRH++  C I G IDFIFG  QS++E C + + 
Sbjct: 157 AAMMLGDKYAIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLR 216

Query: 214 VASLP--QVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIM 273
           V   P  +VY G ITAQ R S  D  GFVFK+CT+ GSGKALLGRA+  +SRVIF  ++ 
Sbjct: 217 VGIYPPNEVY-GTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMF 276

Query: 274 GSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHLTGFSVESFIN 333
              + P GW AW  KG+E +  +VE  CTG GA TS RVPW        +  F+  +FI+
Sbjct: 277 SDNILPIGWDAWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFID 332

Query: 334 QDGWI 337
           ++GW+
Sbjct: 337 EEGWL 332

BLAST of CSPI02G04780 vs. TAIR10
Match: AT5G26810.1 (AT5G26810.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 258.1 bits (658), Expect = 7.8e-69
Identity = 128/245 (52.24%), Postives = 161/245 (65.71%), Query Frame = 1

Query: 98  TVITFDDHQQTDTSATFTSRPPNIIVRGITFENSFNLREAPELFSCDDGTY-ITQAIAAR 157
           T IT++ H  TD S+TFTS P +I+VR ++  N++N      L   +  ++ I  A+A  
Sbjct: 48  TTITYNGHAATDVSSTFTSYPSHIVVRNLSIMNTYN--RLTSLTKANGMSWDIKPAVAIS 107

Query: 158 IYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVAS 217
           +YGDKSAF+NC F G QDT+WD  GRH F +CYIEGAIDFIFGSGQSVYEDC IN    +
Sbjct: 108 VYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYIEGAIDFIFGSGQSVYEDCHINATAGA 167

Query: 218 LP-QVYQGYITAQSRQSAADPSGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVV 277
           L  +V  GYITAQ R S +DPSGFVF   ++ GS    LGRAYGPFSRVIF    + SVV
Sbjct: 168 LASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGSTSVYLGRAYGPFSRVIFIQTDLSSVV 227

Query: 278 APEGWYAWHFKGKEENFMYVEENCTGPGASTSMRVPWAKTLDASHL-TGFSVESFINQDG 337
            PEGWY+WH+ G E +F Y E  C G G+  S RVPW   L + +    FS+ +FI+QD 
Sbjct: 228 HPEGWYSWHYGGYEMSFTYAEVECKGAGSDMSRRVPWIDKLHSFYTKQQFSISNFIDQDQ 287

Query: 338 WIPTV 340
           WI  +
Sbjct: 288 WISNI 290

BLAST of CSPI02G04780 vs. NCBI nr
Match: gi|700205889|gb|KGN61008.1| (hypothetical protein Csa_2G034610 [Cucumis sativus])

HSP 1 Score: 705.3 bits (1819), Expect = 5.2e-200
Identity = 338/340 (99.41%), Postives = 340/340 (100.00%), Query Frame = 1

Query: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPP 60
           MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLT+QS+VIVDKSGNGNFQTVQAAIDSVPP
Sbjct: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTIQSVVIVDKSGNGNFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120
           NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN
Sbjct: 61  NNNHWIKIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPN 120

Query: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180
           IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ
Sbjct: 121 IIVRGITFENSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQ 180

Query: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240
           GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV
Sbjct: 181 GRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFV 240

Query: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300
           FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT
Sbjct: 241 FKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCT 300

Query: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL 341
           GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL
Sbjct: 301 GPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWIPTVL 340

BLAST of CSPI02G04780 vs. NCBI nr
Match: gi|659071556|ref|XP_008460603.1| (PREDICTED: probable pectinesterase 66 [Cucumis melo])

HSP 1 Score: 600.5 bits (1547), Expect = 1.8e-168
Identity = 303/387 (78.29%), Postives = 317/387 (81.91%), Query Frame = 1

Query: 1   MQVQPPFLLLFTLFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPP 60
           MQ+QPPFLLLFTLFNAVVFGFEAVN AR F LTVQS+V+VDKSG G+FQTVQAAIDSVPP
Sbjct: 1   MQLQPPFLLLFTLFNAVVFGFEAVNHARAFDLTVQSVVVVDKSGRGDFQTVQAAIDSVPP 60

Query: 61  NNNHWIKIQINPGVY------KEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATF 120
           NNN WIKIQINPGVY      +EKVTIP EKPFIYLEGAD SNT+ITFDDHQQTDTSATF
Sbjct: 61  NNNQWIKIQINPGVYNWQTFCREKVTIPPEKPFIYLEGADRSNTIITFDDHQQTDTSATF 120

Query: 121 TSRPPNIIVRGITFE-------------------------NSFNLREAPELFSCDDGTYI 180
           TSRPPNII RGITFE                         NSFNL EAPELF CDDGTYI
Sbjct: 121 TSRPPNIIARGITFEVLWLLKTEFIFIASFEILKPKIVAFNSFNLEEAPELFVCDDGTYI 180

Query: 181 TQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCYIEGAIDFIFGSGQSVYE--- 240
           TQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFF +CYIEGAIDFIFGSGQSVY+   
Sbjct: 181 TQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFQNCYIEGAIDFIFGSGQSVYKVLS 240

Query: 241 ----------------DCMINVNVASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGSG 300
                           DC+INVNVASLPQV+QG+ITAQ R+SAADPSGFVFK CTI GSG
Sbjct: 241 SNVNVSVIYTIGRDFYDCLINVNVASLPQVHQGFITAQGRRSAADPSGFVFKGCTITGSG 300

Query: 301 KALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMRV 338
           KALLGRAYGPFSRVIFKDAIMG VVAPEGW AW+FK KE+NFMYVEENCTGPGASTSMRV
Sbjct: 301 KALLGRAYGPFSRVIFKDAIMGPVVAPEGWDAWNFKSKEKNFMYVEENCTGPGASTSMRV 360

BLAST of CSPI02G04780 vs. NCBI nr
Match: gi|778674025|ref|XP_011650111.1| (PREDICTED: putative pectinesterase 52 [Cucumis sativus])

HSP 1 Score: 451.4 bits (1160), Expect = 1.4e-123
Identity = 211/211 (100.00%), Postives = 211/211 (100.00%), Query Frame = 1

Query: 130 NSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCY 189
           NSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCY
Sbjct: 3   NSFNLREAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFSHCY 62

Query: 190 IEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGS 249
           IEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGS
Sbjct: 63  IEGAIDFIFGSGQSVYEDCMINVNVASLPQVYQGYITAQSRQSAADPSGFVFKECTIKGS 122

Query: 250 GKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMR 309
           GKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMR
Sbjct: 123 GKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGASTSMR 182

Query: 310 VPWAKTLDASHLTGFSVESFINQDGWIPTVL 341
           VPWAKTLDASHLTGFSVESFINQDGWIPTVL
Sbjct: 183 VPWAKTLDASHLTGFSVESFINQDGWIPTVL 213

BLAST of CSPI02G04780 vs. NCBI nr
Match: gi|590644768|ref|XP_007031173.1| (Pectinesterase, putative [Theobroma cacao])

HSP 1 Score: 368.6 bits (945), Expect = 1.2e-98
Identity = 186/331 (56.19%), Postives = 226/331 (68.28%), Query Frame = 1

Query: 8   LLLFTLFNAVVFGFEAVNQARDFVLTVQSIVIVDKSGNGNFQTVQAAIDSVPPNNNHWIK 67
           LL+ +L  ++     A N    F  T+    +VDKSG GNF ++Q+AIDS+P NN  WIK
Sbjct: 11  LLILSLRTSMADDCSAGNGGSQFASTI----VVDKSGKGNFNSIQSAIDSIPSNNAQWIK 70

Query: 68  IQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPNIIVRGIT 127
           ++INPGVY EKV IP++KP I LEG D S T ITF  H +TD S TFTS   NI+ +GIT
Sbjct: 71  VRINPGVYTEKVVIPIDKPCIVLEGQDRSVTTITFYAHDRTDRSCTFTSFADNIVAKGIT 130

Query: 128 FENSFNLREAPELFSCDDGTY-ITQAIAARIYGDKSAFFNCGFKGYQDTLWDVQGRHFFS 187
           F+NS+N     E  S +     ++QA+AARI GDKSAFF CGF G QDTLWD  GRH+F 
Sbjct: 131 FKNSYNHLLLLERLSSERPIPGVSQAVAARILGDKSAFFQCGFLGLQDTLWDAIGRHYFY 190

Query: 188 HCYIEGAIDFIFGSGQSVYEDCMINVNVASLP-QVYQGYITAQSRQSAADPSGFVFKECT 247
            C+IEGA+DFIFG GQS+YEDC INV   +   Q+  GYITAQ RQS+ DPSGFVFK   
Sbjct: 191 QCHIEGAVDFIFGYGQSLYEDCSINVTAGAFSSQIPLGYITAQGRQSSNDPSGFVFKRGE 250

Query: 248 IKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVEENCTGPGAS 307
           I G+ +  LGRAYGP+SRVIF++  M + V  EGW AW + GKEENFMY E NC G G+ 
Sbjct: 251 IFGNTQPYLGRAYGPYSRVIFQETTMNAEVVSEGWDAWRYPGKEENFMYAEVNCQGAGSD 310

Query: 308 TSMRVPWAKTLDASHLTGFSVESFINQDGWI 337
           TS RVPW K L+ S L  FS  SFI+ DGWI
Sbjct: 311 TSRRVPWEKKLNPSQLNQFSRSSFIDNDGWI 337

BLAST of CSPI02G04780 vs. NCBI nr
Match: gi|568837944|ref|XP_006472977.1| (PREDICTED: putative pectinesterase 52 [Citrus sinensis])

HSP 1 Score: 366.7 bits (940), Expect = 4.4e-98
Identity = 190/340 (55.88%), Postives = 230/340 (67.65%), Query Frame = 1

Query: 8   LLLFTLFNAVVFGFEAVNQARDFV-LTVQSIVIVDKSGNGNFQTVQAAIDSVPPNNNHWI 67
           L++FTL + +   F A    +D     V   V VD+S +  ++TVQ+AIDS+P  NN WI
Sbjct: 8   LIVFTLLSTI--SFRAARADQDCKGSNVAYTVTVDQSSS-QYRTVQSAIDSIPAENNQWI 67

Query: 68  KIQINPGVYKEKVTIPLEKPFIYLEGADSSNTVITFDDHQQTDTSATFTSRPPNIIVRGI 127
           K+ I  G YKEKV I   KP I LEG  S  T IT+DDHQ TDTSATF+S   N++ +GI
Sbjct: 68  KVHIKAGTYKEKVKIQRNKPCILLEGEGSGVTKITYDDHQSTDTSATFSSFADNVVAKGI 127

Query: 128 TFENSFNL---------REAPELFSCDDGTYITQAIAARIYGDKSAFFNCGFKGYQDTLW 187
           TFENS+NL         R   +L +   G  ITQA+AARIYGDKSAF++C F G QDTLW
Sbjct: 128 TFENSYNLAVEILAELDRRDIDLGTLQAGNDITQALAARIYGDKSAFYDCRFLGVQDTLW 187

Query: 188 DVQGRHFFSHCYIEGAIDFIFGSGQSVYEDCMINVNVASL-PQVYQGYITAQSRQSAADP 247
           DVQGRHFF  CYIEGAIDFIFG+GQSVYEDC +N     L  +   GYITAQSR+SA DP
Sbjct: 188 DVQGRHFFKSCYIEGAIDFIFGNGQSVYEDCSVNSTAGLLFGRGVSGYITAQSRESANDP 247

Query: 248 SGFVFKECTIKGSGKALLGRAYGPFSRVIFKDAIMGSVVAPEGWYAWHFKGKEENFMYVE 307
            GFVF+   + G+G+A LGRAYGP+SRVIF +A + SVV P GW AW+ +G+E NFMY E
Sbjct: 248 GGFVFRGGEVTGTGQAYLGRAYGPYSRVIFYNAWLSSVVTPPGWNAWNLQGQEGNFMYAE 307

Query: 308 ENCTGPGASTSMRVPWAKTLDASHLTGFSVESFINQDGWI 337
            NC GPG+ TS RV W K +D   L  +S   FINQDGWI
Sbjct: 308 VNCKGPGSDTSNRVSWEKKIDPKLLYKYSTSYFINQDGWI 344

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME66_ARATH1.7e-8146.67Probable pectinesterase 66 OS=Arabidopsis thaliana GN=PME66 PE=2 SV=2[more]
PME55_ARATH9.2e-8048.49Probable pectinesterase 55 OS=Arabidopsis thaliana GN=PME55 PE=2 SV=1[more]
PME29_ARATH1.0e-7847.35Probable pectinesterase 29 OS=Arabidopsis thaliana GN=PME29 PE=2 SV=1[more]
PME10_ARATH6.2e-7646.23Putative pectinesterase 10 OS=Arabidopsis thaliana GN=PME10 PE=2 SV=1[more]
PME52_ARATH1.4e-6752.24Putative pectinesterase 52 OS=Arabidopsis thaliana GN=PME52 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0LH00_CUCSA3.6e-20099.41Pectinesterase OS=Cucumis sativus GN=Csa_2G034610 PE=4 SV=1[more]
A0A061F313_THECC8.1e-9956.19Pectinesterase OS=Theobroma cacao GN=TCM_026775 PE=4 SV=1[more]
V4T5U1_9ROSI3.8e-9658.58Pectinesterase (Fragment) OS=Citrus clementina GN=CICLE_v10003837mg PE=4 SV=1[more]
B9STE8_RICCO1.4e-9551.16Pectinesterase OS=Ricinus communis GN=RCOM_1318720 PE=4 SV=1[more]
A0A067GSJ9_CITSI5.4e-9556.19Pectinesterase OS=Citrus sinensis GN=CISIN_1g038332mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G47280.19.5e-8346.67 Pectin lyase-like superfamily protein[more]
AT5G18990.15.2e-8148.49 Pectin lyase-like superfamily protein[more]
AT3G24130.15.8e-8047.35 Pectin lyase-like superfamily protein[more]
AT2G19150.13.5e-7746.23 Pectin lyase-like superfamily protein[more]
AT5G26810.17.8e-6952.24 Pectin lyase-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|700205889|gb|KGN61008.1|5.2e-20099.41hypothetical protein Csa_2G034610 [Cucumis sativus][more]
gi|659071556|ref|XP_008460603.1|1.8e-16878.29PREDICTED: probable pectinesterase 66 [Cucumis melo][more]
gi|778674025|ref|XP_011650111.1|1.4e-123100.00PREDICTED: putative pectinesterase 52 [Cucumis sativus][more]
gi|590644768|ref|XP_007031173.1|1.2e-9856.19Pectinesterase, putative [Theobroma cacao][more]
gi|568837944|ref|XP_006472977.1|4.4e-9855.88PREDICTED: putative pectinesterase 52 [Citrus sinensis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000070Pectinesterase_cat
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005618 cell wall
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G04780.1CSPI02G04780.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 38..332
score: 3.5
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 33..338
score: 8.05
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 35..337
score: 1.2E
NoneNo IPR availablePANTHERPTHR31321FAMILY NOT NAMEDcoord: 6..336
score: 4.3E
NoneNo IPR availablePANTHERPTHR31321:SF32PECTINESTERASE 52-RELATEDcoord: 6..336
score: 4.3E

The following gene(s) are paralogous to this gene:

None