CSPI02G02650 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI02G02650
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionProlyl 4-hydroxylase subunit alpha-2
LocationChr2 : 1824846 .. 1826968 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAATATCTAAAGGGAAGTACATCAAGTTACAGGGTAGGAAATGGTCCACATTCCAGCTTTCGAAGATGATCATGGCCCTCGTTTTGGCACTTGGGTTTTTCATGCTTATTGCTCTTCGCTTCTTCTCTCCTCCTGAAACTTCTCACCACCGTTTCCCTTCCGTCCGGCATACAGCATTTCTAAGGTAATCTGGTAATGCAATGGGTGTGATTCTTTAAGCTTGTTTGTTACAGGAATCAACTGATTTTGATTTCCATTTTGGTAATATTGTTGTTGTTGTAGTGATGGGTTGGGGAAGAGAGGGGATCAGTGGGTTGAGTTCATTTCTTGGGAGCCTAGAGCTTTTGTATATCATAATTTCTTGGTAAGTTTTTAGAATTGTGGTTTTTTTTTGGAAAAAAAAAGGAAATTTTGTTTTGTTAAAATGGTTGGCTGATATTGGAATGACGTTGGACAGTCCAAAGAAGAATGCTTGTACTTGATTAGTCTTGCAAAACCCCACATGGAGAAATCAACTGTGGTTGATAGCAAAACTGGCGAGAGTGTGGATAGCAGGTATGTCTTTATCATTTTCTTTGTTGTCCTTTTACTAACAGAAACAGAACTTGTCAATTATTGACTATGAACTAACTCTGGTGGGGCTTACACTATGACACATTTAAAAGTGTAAGAAAAAGTAATCTCAAAGAGAGTAAATTTGAACTAAACTCATATTTTAAAGATGATTTATCTATATTTAGTCCTTTCTCATAAGTTATCTCTTTAGGAATTGATTGGTCGGTTCGGTTCTTGGAAAATCAGTTCGGTAAAAGTCCAACCTGAACTGACTACAATAGAACGGTTATGTATAATAGAAGGGTATAAAGTAGAACAATACAATTACAATGAACAAGGCGAACTTATAACTTTTTTCTGTACAATGCCATGTCCACAGGGTGCGAACCAGTTCTGGGATGTTTCTGAATAGAGGGCAGGACAAAATCATCAGAAACATAGAGAAAAGAATAGCAGATTTTACATTCATTCCCATAGGTAAATAATTCAAAACTTTCATATCACCACGGCTTATTTAGAAATACTTTTCAAAACAGTTTTTGAAAACATGTTCGAACCACTGTTTTTTGCTAGTTGTTTTATTTTGTCTGTGTTAGTGATTTCATTCAATATATCAATCTTTACAATGCATTCAACTGTAGTTATATTGAATAATTCTTCAAAATAGAGTTGCAACCAGAACATAATTCTCAAACAAATCTTTGTTGGACTAAACAAACATTCGCGAAAAATTAGTTAAAATTCATGTGGGATTTTGTTCATTATTCTTGTTTTTGGCCCAAACCAAAGAGGGCTTTTGTGAATGAGCTATTCATTTGCTGCTTGTTGTTTTGTTGATATCACTGGCATTTTGGTCTTAATACATTTTGGTCTTAATACATTTTGGTTAGTGTTTGATTATTAGATTATTAGACTTGTCTCGTTTATTATCATTAACAAAGAGGATTGTTTCTGTTAAAAACAACATTTTGGTAATGATATTCACAATTCCACACAGAGCATGGAGAAGGACTTCAAATTCTCCATTATGAAGTTGGGCAGAAATATGATGCGCATTATGATTACTTTGTTGATGAGTACAACATCAAAAAAGGAGGCCAAAGAATGGCCACCCTTCTCATGTATTTGTAAGATTCTACTTTGACGATCTCTCAAACTCATCTACCCTCTTCTCTAGTTTCTCTCTGAATCGATACCAATGGCAATGCAGGTCCGATGTTGAAGAGGGGGGCGAGACGGTGTTCCCAGCTGCAAAAGGAAACTTCAGCTCTGTGCCATGGTGGAATGAACTATCTGAATGTGGCAAAGGTGGACTCTCTGTAAAACCAAAAATGGGAGATGCTTTATTGTTCTGGAGCATGAAGCCCGATGCTACCTTAGACCCTACAAGTTTACATGGTTAGTCCTTTTTCCTTTTCCTCCCTATTTAACTTTAGCATTAGAAGATTGGTTACATCTAAATTATCCTCCCTAATCTTGTTTTTGATATGTAGGTGCCTGTCCTGTCATTAGAGGGAACAAATGGTCATGTACAAAGTGGATGCATGTTGATAAATATATTTAA

mRNA sequence

ATGGCAATATCTAAAGGGAAGTACATCAAGTTACAGGGTAGGAAATGGTCCACATTCCAGCTTTCGAAGATGATCATGGCCCTCGTTTTGGCACTTGGGTTTTTCATGCTTATTGCTCTTCGCTTCTTCTCTCCTCCTGAAACTTCTCACCACCGTTTCCCTTCCGTCCGGCATACAGCATTTCTAAGTGATGGGTTGGGGAAGAGAGGGGATCAGTGGGTTGAGTTCATTTCTTGGGAGCCTAGAGCTTTTGTATATCATAATTTCTTGTCCAAAGAAGAATGCTTGTACTTGATTAGTCTTGCAAAACCCCACATGGAGAAATCAACTGTGGTTGATAGCAAAACTGGCGAGAGTGTGGATAGCAGGGTGCGAACCAGTTCTGGGATGTTTCTGAATAGAGGGCAGGACAAAATCATCAGAAACATAGAGAAAAGAATAGCAGATTTTACATTCATTCCCATAGAGCATGGAGAAGGACTTCAAATTCTCCATTATGAAGTTGGGCAGAAATATGATGCGCATTATGATTACTTTGTTGATGAGTACAACATCAAAAAAGGAGGCCAAAGAATGGCCACCCTTCTCATGTATTTGTCCGATGTTGAAGAGGGGGGCGAGACGGTGTTCCCAGCTGCAAAAGGAAACTTCAGCTCTGTGCCATGGTGGAATGAACTATCTGAATGTGGCAAAGGTGGACTCTCTGTAAAACCAAAAATGGGAGATGCTTTATTGTTCTGGAGCATGAAGCCCGATGCTACCTTAGACCCTACAAGTTTACATGGTGCCTGTCCTGTCATTAGAGGGAACAAATGGTCATGTACAAAGTGGATGCATGTTGATAAATATATTTAA

Coding sequence (CDS)

ATGGCAATATCTAAAGGGAAGTACATCAAGTTACAGGGTAGGAAATGGTCCACATTCCAGCTTTCGAAGATGATCATGGCCCTCGTTTTGGCACTTGGGTTTTTCATGCTTATTGCTCTTCGCTTCTTCTCTCCTCCTGAAACTTCTCACCACCGTTTCCCTTCCGTCCGGCATACAGCATTTCTAAGTGATGGGTTGGGGAAGAGAGGGGATCAGTGGGTTGAGTTCATTTCTTGGGAGCCTAGAGCTTTTGTATATCATAATTTCTTGTCCAAAGAAGAATGCTTGTACTTGATTAGTCTTGCAAAACCCCACATGGAGAAATCAACTGTGGTTGATAGCAAAACTGGCGAGAGTGTGGATAGCAGGGTGCGAACCAGTTCTGGGATGTTTCTGAATAGAGGGCAGGACAAAATCATCAGAAACATAGAGAAAAGAATAGCAGATTTTACATTCATTCCCATAGAGCATGGAGAAGGACTTCAAATTCTCCATTATGAAGTTGGGCAGAAATATGATGCGCATTATGATTACTTTGTTGATGAGTACAACATCAAAAAAGGAGGCCAAAGAATGGCCACCCTTCTCATGTATTTGTCCGATGTTGAAGAGGGGGGCGAGACGGTGTTCCCAGCTGCAAAAGGAAACTTCAGCTCTGTGCCATGGTGGAATGAACTATCTGAATGTGGCAAAGGTGGACTCTCTGTAAAACCAAAAATGGGAGATGCTTTATTGTTCTGGAGCATGAAGCCCGATGCTACCTTAGACCCTACAAGTTTACATGGTGCCTGTCCTGTCATTAGAGGGAACAAATGGTCATGTACAAAGTGGATGCATGTTGATAAATATATTTAA
Homology
[Close]
BLAST of CSPI02G02650 vs. Swiss-Prot
Match: P4H3_ARATH (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana GN=P4H3 PE=2 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 2.1e-117
Identity = 204/286 (71.33%), Postives = 230/286 (80.42%), Query Frame = 1 

Query: 3   ISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFPSV-----R 62
           ++K ++ + Q RKWST  L  + M  +L +   ML+A   FS P  +    P       R
Sbjct: 1   MAKLRHSRFQARKWSTLMLV-LFMLFMLTIVLLMLLAFGVFSLPINNDESSPIDLSYFRR 60

Query: 63  HTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTG 122
                S+GLGKRGDQW E +SWEPRAFVYHNFLSKEEC YLISLAKPHM KSTVVDS+TG
Sbjct: 61  AATERSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETG 120

Query: 123 ESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYD 182
           +S DSRVRTSSG FL RG+DKII+ IEKRIAD+TFIP +HGEGLQ+LHYE GQKY+ HYD
Sbjct: 121 KSKDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYD 180

Query: 183 YFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVK 242
           YFVDE+N K GGQRMAT+LMYLSDVEEGGETVFPAA  NFSSVPW+NELSECGK GLSVK
Sbjct: 181 YFVDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVK 240

Query: 243 PKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           P+MGDALLFWSM+PDATLDPTSLHG CPVIRGNKWS TKWMHV +Y
Sbjct: 241 PRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEY 285

[Close]
BLAST of CSPI02G02650 vs. Swiss-Prot
Match: P4H10_ARATH (Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana GN=P4H10 PE=2 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 3.0e-108
Identity = 187/270 (69.26%), Postives = 216/270 (80.00%), Query Frame = 1 

Query: 22  SKMIMALVLALGFFMLIALRFFSPPETSHHRFPS--------VRHTAFLSDGLGKRGDQW 81
           S ++ A+++   F +LI L F      S++   S        VR T   S     + ++W
Sbjct: 18  STLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIVRKTLQRSGEDDSKNERW 77

Query: 82  VEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLN 141
           VE ISWEPRA VYHNFL+KEEC YLI LAKPHMEKSTVVD KTG+S DSRVRTSSG FL 
Sbjct: 78  VEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKTGKSTDSRVRTSSGTFLA 137

Query: 142 RGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMA 201
           RG+DK IR IEKRI+DFTFIP+EHGEGLQ+LHYE+GQKY+ HYDYF+DEYN + GGQR+A
Sbjct: 138 RGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHYDYFMDEYNTRNGGQRIA 197

Query: 202 TLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDA 261
           T+LMYLSDVEEGGETVFPAAKGN+S+VPWWNELSECGKGGLSVKPKMGDALLFWSM PDA
Sbjct: 198 TVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSVKPKMGDALLFWSMTPDA 257

Query: 262 TLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           TLDP+SLHG C VI+GNKWS TKW+ V +Y
Sbjct: 258 TLDPSSLHGGCAVIKGNKWSSTKWLRVHEY 287

[Close]
BLAST of CSPI02G02650 vs. Swiss-Prot
Match: P4H8_ARATH (Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana GN=P4H8 PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 7.9e-101
Identity = 178/289 (61.59%), Postives = 219/289 (75.78%), Query Frame = 1 

Query: 5   KGKYIKLQGRK-WSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHR---------FP 64
           K K ++ + RK +ST   + +++ L + L   +L+ L  FS P T+              
Sbjct: 4   KPKQLRNKPRKSFSTQTFTVVVLVLFVIL---ILVGLGIFSLPSTNKTSSMPMDLTTIVQ 63

Query: 65  SVRHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS 124
           +++      D     GD+W+E ISWEPRAFVYHNFL+ EEC +LISLAKP M KS VVD 
Sbjct: 64  TIQERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDV 123

Query: 125 KTGESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDA 184
           KTG+S+DSRVRTSSG FLNRG D+I+  IE RI+DFTFIP E+GEGLQ+LHYEVGQ+Y+ 
Sbjct: 124 KTGKSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEP 183

Query: 185 HYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGL 244
           H+DYF DE+N++KGGQR+AT+LMYLSDV+EGGETVFPAAKGN S VPWW+ELS+CGK GL
Sbjct: 184 HHDYFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGL 243

Query: 245 SVKPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           SV PK  DALLFWSMKPDA+LDP+SLHG CPVI+GNKWS TKW HV +Y
Sbjct: 244 SVLPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHEY 289

[Close]
BLAST of CSPI02G02650 vs. Swiss-Prot
Match: P4H5_ARATH (Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana GN=P4H5 PE=2 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 2.6e-99
Identity = 171/289 (59.17%), Postives = 216/289 (74.74%), Query Frame = 1 

Query: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFPS----- 60
           MA    ++++ Q RK  +       + ++L +   +L+ L   S P  + +   +     
Sbjct: 1   MASKSKQHLRYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTN 60

Query: 61  -VRHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS 120
            VR +   S      G++WVE ISWEPRA VYHNFL+ EEC +LISLAKP M KSTVVD 
Sbjct: 61  IVRKSETSSGDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDE 120

Query: 121 KTGESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDA 180
           KTG S DSRVRTSSG FL RG D+++  IEKRI+DFTFIP+E+GEGLQ+LHY+VGQKY+ 
Sbjct: 121 KTGGSKDSRVRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEP 180

Query: 181 HYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGL 240
           HYDYF+DE+N K GGQR+AT+LMYLSDV++GGETVFPAA+GN S+VPWWNELS+CGK GL
Sbjct: 181 HYDYFLDEFNTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGL 240

Query: 241 SVKPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           SV PK  DALLFW+M+PDA+LDP+SLHG CPV++GNKWS TKW HV ++
Sbjct: 241 SVLPKKRDALLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEF 289

[Close]
BLAST of CSPI02G02650 vs. Swiss-Prot
Match: P4H7_ARATH (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana GN=P4H7 PE=2 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 3.4e-67
Identity = 116/207 (56.04%), Postives = 157/207 (75.85%), Query Frame = 1 

Query: 77  ISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLNRGQ 136
           +SW PR F+Y  FLS EEC + I LAK  +EKS V D+ +GESV+S VRTSSGMFL++ Q
Sbjct: 59  LSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKRQ 118

Query: 137 DKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMATLL 196
           D I+ N+E ++A +TF+P E+GE +QILHYE GQKY+ H+DYF D+ N++ GG R+AT+L
Sbjct: 119 DDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATVL 178

Query: 197 MYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDATLD 256
           MYLS+VE+GGETVFP  KG  + +   +  +EC K G +VKP+ GDALLF+++ P+AT D
Sbjct: 179 MYLSNVEKGGETVFPMWKGKATQLK-DDSWTECAKQGYAVKPRKGDALLFFNLHPNATTD 238

Query: 257 PTSLHGACPVIRGNKWSCTKWMHVDKY 284
             SLHG+CPV+ G KWS T+W+HV  +
Sbjct: 239 SNSLHGSCPVVEGEKWSATRWIHVKSF 264

[Close]
BLAST of CSPI02G02650 vs. TrEMBL
Match: A0A0A0LFF5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G009620 PE=4 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 2.6e-151
Identity = 257/259 (99.23%), Postives = 257/259 (99.23%), Query Frame = 1 

Query: 26  MALVLALGFFMLIALRFFSPPETSHHRFPSVRHTAFLSDGLGKRGDQWVEFISWEPRAFV 85
           MALVLALGFFMLIALRF SPPETSHHRF SVRHTAFLSDGLGKRGDQWVEFISWEPRAFV
Sbjct: 1   MALVLALGFFMLIALRFLSPPETSHHRFSSVRHTAFLSDGLGKRGDQWVEFISWEPRAFV 60

Query: 86  YHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLNRGQDKIIRNIEK 145
           YHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLNRGQDKIIRNIEK
Sbjct: 61  YHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLNRGQDKIIRNIEK 120

Query: 146 RIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMATLLMYLSDVEEG 205
           RIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMATLLMYLSDVEEG
Sbjct: 121 RIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMATLLMYLSDVEEG 180

Query: 206 GETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDATLDPTSLHGACP 265
           GETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDATLDPTSLHGACP
Sbjct: 181 GETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDATLDPTSLHGACP 240

Query: 266 VIRGNKWSCTKWMHVDKYI 285
           VIRGNKWSCTKWMHVDKYI
Sbjct: 241 VIRGNKWSCTKWMHVDKYI 259

[Close]
BLAST of CSPI02G02650 vs. TrEMBL
Match: A0A061FEJ2_THECC (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein isoform 1 OS=Theobroma cacao GN=TCM_034293 PE=4 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 1.7e-118
Identity = 207/290 (71.38%), Postives = 238/290 (82.07%), Query Frame = 1 

Query: 3   ISKGKYIKLQGRKWSTFQLS-------KMIMALVLALGFFMLIALRFFSPPE--TSHHRF 62
           ++K ++ +LQ +KWST  L         +++ ++L LG F L      SPP   TS+ R 
Sbjct: 1   MAKVRHSRLQAKKWSTVMLVLSMLFMLTVVLLMLLGLGIFSLPMSTDDSPPNDLTSYRRM 60

Query: 63  PSVRHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVD 122
            S R        LGKRG+QW E +SWEPRAF+YHNFLSKEEC YLI+LAKPHM KSTVVD
Sbjct: 61  ASER-----GKELGKRGEQWTEVLSWEPRAFIYHNFLSKEECEYLINLAKPHMAKSTVVD 120

Query: 123 SKTGESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYD 182
           SKTG S DSRVRTSSGMFL RGQDKIIR+IEKRIAD+TFIP+EHGEGLQ+LHYEVGQKYD
Sbjct: 121 SKTGRSKDSRVRTSSGMFLRRGQDKIIRDIEKRIADYTFIPVEHGEGLQVLHYEVGQKYD 180

Query: 183 AHYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGG 242
           AH+DYF+DE+N K GGQRMAT+LMYLSDVEEGGET+FPAAKGNFS+VPWWNELSECGK G
Sbjct: 181 AHFDYFLDEFNTKNGGQRMATMLMYLSDVEEGGETIFPAAKGNFSAVPWWNELSECGKQG 240

Query: 243 LSVKPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           LSVKPKMGDALLFWSM+PDATLDP+SLHG CPVI GNKWS TKW+HV++Y
Sbjct: 241 LSVKPKMGDALLFWSMRPDATLDPSSLHGGCPVIMGNKWSSTKWIHVEEY 285

[Close]
BLAST of CSPI02G02650 vs. TrEMBL
Match: E0CQW5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g00950 PE=4 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 2.2e-118
Identity = 206/287 (71.78%), Postives = 239/287 (83.28%), Query Frame = 1 

Query: 3   ISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPP-----ETSHHRFPSVR 62
           ++KG+Y +  G++WST  L   ++ L+L +   ML+AL   S P       + +   S R
Sbjct: 1   MAKGRYSRGHGKRWSTLALVLSLL-LMLTVVLLMLLALGIVSLPIGTVDSDAANDLSSFR 60

Query: 63  HTAFLS-DGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKT 122
              F   +GLGKRG+QW E +SWEPRAF+YHNFLSKEEC Y+ISLAKP+M+KSTVVDS+T
Sbjct: 61  RKTFDGGEGLGKRGEQWTEIVSWEPRAFIYHNFLSKEECEYMISLAKPYMKKSTVVDSET 120

Query: 123 GESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 182
           G S DSRVRTSSGMFL RG+DKIIR+IEKRIADFTFIP+EHGEGLQ+LHYEVGQKYDAHY
Sbjct: 121 GRSKDSRVRTSSGMFLRRGRDKIIRDIEKRIADFTFIPVEHGEGLQVLHYEVGQKYDAHY 180

Query: 183 DYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSV 242
           DYF+DE+N K GGQR+ATLLMYLSDVEEGGETVFPA K NFSSVPWWNELSECGK GLSV
Sbjct: 181 DYFLDEFNTKNGGQRIATLLMYLSDVEEGGETVFPATKANFSSVPWWNELSECGKKGLSV 240

Query: 243 KPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           KPKMGDALLFWSM+PDATLDP+SLHG CPVI+GNKWS TKWMHV++Y
Sbjct: 241 KPKMGDALLFWSMRPDATLDPSSLHGGCPVIKGNKWSSTKWMHVEEY 286

[Close]
BLAST of CSPI02G02650 vs. TrEMBL
Match: A0A0D2S8Z3_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G269000 PE=4 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 3.2e-117
Identity = 207/291 (71.13%), Postives = 240/291 (82.47%), Query Frame = 1 

Query: 3   ISKGKYIKLQGRKWST--------FQLSKMIMALVLALGFFMLIALRFFSPPE--TSHHR 62
           ++K ++ +LQ RKWST        F LS +++ ++L LG F L      S P   TS+ R
Sbjct: 1   MAKVRHSRLQARKWSTVTLVLSMLFMLS-VVLLMLLGLGVFFLPINDDDSAPNDLTSYRR 60

Query: 63  FPSVRHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVV 122
             S R       GLGKRG+QW E +SWEPRAF+YHNFLSKEEC YLI+LAKPHM KSTVV
Sbjct: 61  MASER-----GKGLGKRGEQWTEVLSWEPRAFIYHNFLSKEECEYLINLAKPHMVKSTVV 120

Query: 123 DSKTGESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKY 182
           DSKTG+S DSRVRTSSGMFL RGQDKII++IEKRIAD++FIP+EHGEGLQ+LHYEVGQKY
Sbjct: 121 DSKTGKSKDSRVRTSSGMFLRRGQDKIIKDIEKRIADYSFIPVEHGEGLQVLHYEVGQKY 180

Query: 183 DAHYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKG 242
           DAH+DYF+DE+N K GGQRMAT+LMYLSDVEEGGET+FPAAKGN SSVPWWNELSECGK 
Sbjct: 181 DAHFDYFLDEFNTKNGGQRMATMLMYLSDVEEGGETIFPAAKGNISSVPWWNELSECGKQ 240

Query: 243 GLSVKPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           GL+VKPKMGDALLFWSM+PDATLDP+SLHG CPVI GNKWS TKWMH+++Y
Sbjct: 241 GLAVKPKMGDALLFWSMRPDATLDPSSLHGGCPVIMGNKWSSTKWMHLEEY 285

[Close]
BLAST of CSPI02G02650 vs. TrEMBL
Match: A0A0B0NIF8_GOSAR (Prolyl 4-hydroxylase subunit alpha-2 OS=Gossypium arboreum GN=F383_02226 PE=4 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 5.5e-117
Identity = 205/291 (70.45%), Postives = 238/291 (81.79%), Query Frame = 1 

Query: 3   ISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPE----------TSHHR 62
           ++K ++ +LQ RKWST  L  + M  +L++   ML+ L  FS P           TS+ R
Sbjct: 1   MAKVRHSRLQARKWSTVTLV-LSMLFMLSVVLLMLLGLGIFSLPINNDDSAPNDLTSYRR 60

Query: 63  FPSVRHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVV 122
             S R       GLGKRG+QW E +SWEPRAF+YHNFLSKEEC YLI+LAKPHM KSTVV
Sbjct: 61  MASER-----GKGLGKRGEQWTEVLSWEPRAFIYHNFLSKEECEYLINLAKPHMVKSTVV 120

Query: 123 DSKTGESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKY 182
           DSKTG+S DSRVRTSSGMFL RGQDKIIR+IE RIAD++FIP+EHGEGLQ+LHYEVGQKY
Sbjct: 121 DSKTGKSKDSRVRTSSGMFLRRGQDKIIRDIENRIADYSFIPVEHGEGLQVLHYEVGQKY 180

Query: 183 DAHYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKG 242
           DAH+DYF+DE+N K GGQRMAT+LMYLSDVEEGGET+FPAAKGN S+VPWWNELSECGK 
Sbjct: 181 DAHFDYFLDEFNTKNGGQRMATMLMYLSDVEEGGETIFPAAKGNISAVPWWNELSECGKQ 240

Query: 243 GLSVKPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           GL+VKPKMGDALLFWSM+PDATLDP+SLHG CPVI GNKWS TKWMH+++Y
Sbjct: 241 GLAVKPKMGDALLFWSMRPDATLDPSSLHGGCPVITGNKWSSTKWMHLEEY 285

[Close]
BLAST of CSPI02G02650 vs. TAIR10
Match: AT1G20270.1 (AT1G20270.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 423.3 bits (1087), Expect = 1.2e-118
Identity = 204/286 (71.33%), Postives = 230/286 (80.42%), Query Frame = 1 

Query: 3   ISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFPSV-----R 62
           ++K ++ + Q RKWST  L  + M  +L +   ML+A   FS P  +    P       R
Sbjct: 1   MAKLRHSRFQARKWSTLMLV-LFMLFMLTIVLLMLLAFGVFSLPINNDESSPIDLSYFRR 60

Query: 63  HTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTG 122
                S+GLGKRGDQW E +SWEPRAFVYHNFLSKEEC YLISLAKPHM KSTVVDS+TG
Sbjct: 61  AATERSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETG 120

Query: 123 ESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYD 182
           +S DSRVRTSSG FL RG+DKII+ IEKRIAD+TFIP +HGEGLQ+LHYE GQKY+ HYD
Sbjct: 121 KSKDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYD 180

Query: 183 YFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVK 242
           YFVDE+N K GGQRMAT+LMYLSDVEEGGETVFPAA  NFSSVPW+NELSECGK GLSVK
Sbjct: 181 YFVDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVK 240

Query: 243 PKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           P+MGDALLFWSM+PDATLDPTSLHG CPVIRGNKWS TKWMHV +Y
Sbjct: 241 PRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEY 285

[Close]
BLAST of CSPI02G02650 vs. TAIR10
Match: AT5G66060.1 (AT5G66060.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 392.9 bits (1008), Expect = 1.7e-109
Identity = 187/270 (69.26%), Postives = 216/270 (80.00%), Query Frame = 1 

Query: 22  SKMIMALVLALGFFMLIALRFFSPPETSHHRFPS--------VRHTAFLSDGLGKRGDQW 81
           S ++ A+++   F +LI L F      S++   S        VR T   S     + ++W
Sbjct: 18  STLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIVRKTLQRSGEDDSKNERW 77

Query: 82  VEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLN 141
           VE ISWEPRA VYHNFL+KEEC YLI LAKPHMEKSTVVD KTG+S DSRVRTSSG FL 
Sbjct: 78  VEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKTGKSTDSRVRTSSGTFLA 137

Query: 142 RGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMA 201
           RG+DK IR IEKRI+DFTFIP+EHGEGLQ+LHYE+GQKY+ HYDYF+DEYN + GGQR+A
Sbjct: 138 RGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHYDYFMDEYNTRNGGQRIA 197

Query: 202 TLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDA 261
           T+LMYLSDVEEGGETVFPAAKGN+S+VPWWNELSECGKGGLSVKPKMGDALLFWSM PDA
Sbjct: 198 TVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSVKPKMGDALLFWSMTPDA 257

Query: 262 TLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           TLDP+SLHG C VI+GNKWS TKW+ V +Y
Sbjct: 258 TLDPSSLHGGCAVIKGNKWSSTKWLRVHEY 287

[Close]
BLAST of CSPI02G02650 vs. TAIR10
Match: AT4G35810.1 (AT4G35810.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 368.2 bits (944), Expect = 4.5e-102
Identity = 178/289 (61.59%), Postives = 219/289 (75.78%), Query Frame = 1 

Query: 5   KGKYIKLQGRK-WSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHR---------FP 64
           K K ++ + RK +ST   + +++ L + L   +L+ L  FS P T+              
Sbjct: 4   KPKQLRNKPRKSFSTQTFTVVVLVLFVIL---ILVGLGIFSLPSTNKTSSMPMDLTTIVQ 63

Query: 65  SVRHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS 124
           +++      D     GD+W+E ISWEPRAFVYHNFL+ EEC +LISLAKP M KS VVD 
Sbjct: 64  TIQERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDV 123

Query: 125 KTGESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDA 184
           KTG+S+DSRVRTSSG FLNRG D+I+  IE RI+DFTFIP E+GEGLQ+LHYEVGQ+Y+ 
Sbjct: 124 KTGKSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEP 183

Query: 185 HYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGL 244
           H+DYF DE+N++KGGQR+AT+LMYLSDV+EGGETVFPAAKGN S VPWW+ELS+CGK GL
Sbjct: 184 HHDYFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGL 243

Query: 245 SVKPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           SV PK  DALLFWSMKPDA+LDP+SLHG CPVI+GNKWS TKW HV +Y
Sbjct: 244 SVLPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHEY 289

[Close]
BLAST of CSPI02G02650 vs. TAIR10
Match: AT2G17720.1 (AT2G17720.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 363.2 bits (931), Expect = 1.4e-100
Identity = 171/289 (59.17%), Postives = 216/289 (74.74%), Query Frame = 1 

Query: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFPS----- 60
           MA    ++++ Q RK  +       + ++L +   +L+ L   S P  + +   +     
Sbjct: 1   MASKSKQHLRYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTN 60

Query: 61  -VRHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS 120
            VR +   S      G++WVE ISWEPRA VYHNFL+ EEC +LISLAKP M KSTVVD 
Sbjct: 61  IVRKSETSSGDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDE 120

Query: 121 KTGESVDSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDA 180
           KTG S DSRVRTSSG FL RG D+++  IEKRI+DFTFIP+E+GEGLQ+LHY+VGQKY+ 
Sbjct: 121 KTGGSKDSRVRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEP 180

Query: 181 HYDYFVDEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGL 240
           HYDYF+DE+N K GGQR+AT+LMYLSDV++GGETVFPAA+GN S+VPWWNELS+CGK GL
Sbjct: 181 HYDYFLDEFNTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGL 240

Query: 241 SVKPKMGDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           SV PK  DALLFW+M+PDA+LDP+SLHG CPV++GNKWS TKW HV ++
Sbjct: 241 SVLPKKRDALLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEF 289

[Close]
BLAST of CSPI02G02650 vs. TAIR10
Match: AT5G18900.1 (AT5G18900.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 250.8 bits (639), Expect = 1.0e-66
Identity = 118/212 (55.66%), Postives = 160/212 (75.47%), Query Frame = 1 

Query: 74  VEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLN 133
           V+ +S +PRAFVY  FL++ EC +++SLAK  +++S V D+ +GES  S VRTSSG F++
Sbjct: 37  VKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGESKFSEVRTSSGTFIS 96

Query: 134 RGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMA 193
           +G+D I+  IE +I+ +TF+P E+GE +Q+L YE GQKYDAH+DYF D+ NI +GG RMA
Sbjct: 97  KGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMA 156

Query: 194 TLLMYLSDVEEGGETVFPAAKGNFSSVPWWN--ELSECGKGGLSVKPKMGDALLFWSMKP 253
           T+LMYLS+V +GGETVFP A+     V   N  +LS+C K G++VKP+ GDALLF+++ P
Sbjct: 157 TILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKRGIAVKPRKGDALLFFNLHP 216

Query: 254 DATLDPTSLHGACPVIRGNKWSCTKWMHVDKY 284
           DA  DP SLHG CPVI G KWS TKW+HVD +
Sbjct: 217 DAIPDPLSLHGGCPVIEGEKWSATKWIHVDSF 248

[Close]
BLAST of CSPI02G02650 vs. NCBI nr
Match: gi|778666404|ref|XP_011648735.1| (PREDICTED: probable prolyl 4-hydroxylase 3 [Cucumis sativus])

HSP 1 Score: 590.9 bits (1522), Expect = 1.2e-165
Identity = 282/284 (99.30%), Postives = 282/284 (99.30%), Query Frame = 1 

Query: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFPSVRHTA 60
           MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRF SPPETSHHRF SVRHTA
Sbjct: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFLSPPETSHHRFSSVRHTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESV 120
           FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESV
Sbjct: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESV 120

Query: 121 DSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180
           DSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV
Sbjct: 121 DSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180

Query: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240
           DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM
Sbjct: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240

Query: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKYI 285
           GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKYI
Sbjct: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKYI 284

[Close]
BLAST of CSPI02G02650 vs. NCBI nr
Match: gi|659070731|ref|XP_008456388.1| (PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like isoform X2 [Cucumis melo])

HSP 1 Score: 581.3 bits (1497), Expect = 9.5e-163
Identity = 276/284 (97.18%), Postives = 280/284 (98.59%), Query Frame = 1 

Query: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFPSVRHTA 60
           MA+SKGKYIKLQG+KWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHR PSVR TA
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRLPSVRRTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESV 120
           F SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTG+SV
Sbjct: 61  FQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGKSV 120

Query: 121 DSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180
           DSRVRTSSGMFLNRGQDKII NIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV
Sbjct: 121 DSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180

Query: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240
           DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM
Sbjct: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240

Query: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKYI 285
           GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHV+KYI
Sbjct: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVNKYI 284

[Close]
BLAST of CSPI02G02650 vs. NCBI nr
Match: gi|659070723|ref|XP_008456352.1| (PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like [Cucumis melo])

HSP 1 Score: 552.0 bits (1421), Expect = 6.2e-154
Identity = 261/284 (91.90%), Postives = 271/284 (95.42%), Query Frame = 1 

Query: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFPSVRHTA 60
           MA+S GKYIKLQG+KWSTFQLSKMIMALVLALGFFML AL FFSPPETSHHR  SVRHTA
Sbjct: 1   MAVSIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLSALWFFSPPETSHHRLSSVRHTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESV 120
           FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDS+TG+SV
Sbjct: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV 120

Query: 121 DSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180
           DS VRTSSGMFLNRGQDKII NIEKRIADFTFIPIEHGE +QILHY VGQKYDAHYD+FV
Sbjct: 121 DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV 180

Query: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240
           DEYN+K  GQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGK GLS+KPKM
Sbjct: 181 DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM 240

Query: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKYI 285
           GDALLFWSMKPD TLDPTSLHGACPVIRGNKWSCTKW+HV+KYI
Sbjct: 241 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNKYI 284

[Close]
BLAST of CSPI02G02650 vs. NCBI nr
Match: gi|700205656|gb|KGN60775.1| (hypothetical protein Csa_2G009620 [Cucumis sativus])

HSP 1 Score: 542.7 bits (1397), Expect = 3.8e-151
Identity = 257/259 (99.23%), Postives = 257/259 (99.23%), Query Frame = 1 

Query: 26  MALVLALGFFMLIALRFFSPPETSHHRFPSVRHTAFLSDGLGKRGDQWVEFISWEPRAFV 85
           MALVLALGFFMLIALRF SPPETSHHRF SVRHTAFLSDGLGKRGDQWVEFISWEPRAFV
Sbjct: 1   MALVLALGFFMLIALRFLSPPETSHHRFSSVRHTAFLSDGLGKRGDQWVEFISWEPRAFV 60

Query: 86  YHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLNRGQDKIIRNIEK 145
           YHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLNRGQDKIIRNIEK
Sbjct: 61  YHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLNRGQDKIIRNIEK 120

Query: 146 RIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMATLLMYLSDVEEG 205
           RIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMATLLMYLSDVEEG
Sbjct: 121 RIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMATLLMYLSDVEEG 180

Query: 206 GETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDATLDPTSLHGACP 265
           GETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDATLDPTSLHGACP
Sbjct: 181 GETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDATLDPTSLHGACP 240

Query: 266 VIRGNKWSCTKWMHVDKYI 285
           VIRGNKWSCTKWMHVDKYI
Sbjct: 241 VIRGNKWSCTKWMHVDKYI 259

[Close]
BLAST of CSPI02G02650 vs. NCBI nr
Match: gi|659070729|ref|XP_008456383.1| (PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like isoform X1 [Cucumis melo])

HSP 1 Score: 531.9 bits (1369), Expect = 6.6e-148
Identity = 258/284 (90.85%), Postives = 264/284 (92.96%), Query Frame = 1 

Query: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRFPSVRHTA 60
           MA+SKGKYIKLQG+KWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHR PSVR TA
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRLPSVRRTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESV 120
           F SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTG+SV
Sbjct: 61  FQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGKSV 120

Query: 121 DSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180
           DSRVRTSSGMFLNRGQDKII NIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV
Sbjct: 121 DSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180

Query: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240
           DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM
Sbjct: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240

Query: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDKYI 285
           GDALLFWSMKPDATLDPTSLHG         W  + W   D ++
Sbjct: 241 GDALLFWSMKPDATLDPTSLHGCNE--NTLCWGRSNWKTTDDFL 282

The following BLAST results are available for this feature:
BLAST of CSPI02G02650 vs. Swiss-Prot
Match NameE-valueIdentityDescription
P4H3_ARATH2.1e-11771.33Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana GN=P4H3 PE=2 SV=1[more]
P4H10_ARATH3.0e-10869.26Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana GN=P4H10 PE=2 SV=1[more]
P4H8_ARATH7.9e-10161.59Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana GN=P4H8 PE=3 SV=1[more]
P4H5_ARATH2.6e-9959.17Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana GN=P4H5 PE=2 SV=1[more]
P4H7_ARATH3.4e-6756.04Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana GN=P4H7 PE=2 SV=1[more]
BLAST of CSPI02G02650 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LFF5_CUCSA2.6e-15199.23Uncharacterized protein OS=Cucumis sativus GN=Csa_2G009620 PE=4 SV=1[more]
A0A061FEJ2_THECC1.7e-11871.382-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein isoform ... [more]
E0CQW5_VITVI2.2e-11871.78Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g00950 PE=4 SV=... [more]
A0A0D2S8Z3_GOSRA3.2e-11771.13Uncharacterized protein OS=Gossypium raimondii GN=B456_009G269000 PE=4 SV=1[more]
A0A0B0NIF8_GOSAR5.5e-11770.45Prolyl 4-hydroxylase subunit alpha-2 OS=Gossypium arboreum GN=F383_02226 PE=4 SV... [more]
BLAST of CSPI02G02650 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G20270.11.2e-11871.33 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT5G66060.11.7e-10969.26 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT4G35810.14.5e-10261.59 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT2G17720.11.4e-10059.17 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT5G18900.11.0e-6655.66 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
BLAST of CSPI02G02650 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778666404|ref|XP_011648735.1|1.2e-16599.30PREDICTED: probable prolyl 4-hydroxylase 3 [Cucumis sativus][more]
gi|659070731|ref|XP_008456388.1|9.5e-16397.18PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like isoform X2 [Cucumis melo][more]
gi|659070723|ref|XP_008456352.1|6.2e-15491.90PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like [Cucumis melo][more]
gi|700205656|gb|KGN60775.1|3.8e-15199.23hypothetical protein Csa_2G009620 [Cucumis sativus][more]
gi|659070729|ref|XP_008456383.1|6.6e-14890.85PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like isoform X1 [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR006620Pro_4_hyd_alph
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506iron ion binding
GO:0031418L-ascorbic acid binding
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005768 endosome
cellular_component GO:0005802 trans-Golgi network
cellular_component GO:0005794 Golgi apparatus
molecular_function GO:0005506 iron ion binding
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G02650.1CSPI02G02650.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF136402OG-FeII_Oxy_3coord: 161..279
score: 1.2
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 157..280
score: 12
IPR006620Prolyl 4-hydroxylase, alpha subunitSMARTSM00702p4hccoord: 81..279
score: 5.2
NoneNo IPR availablePANTHERPTHR10869PROLYL 4-HYDROXYLASE ALPHA SUBUNITcoord: 12..283
score: 6.9E
NoneNo IPR availablePANTHERPTHR10869:SF782-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEINcoord: 12..283
score: 6.9E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI02G02650CmaCh12G011450Cucurbita maxima (Rimu)cmacpiB174
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CSPI02G02650Cucurbita pepo (Zucchini)cpecpiB100
CSPI02G02650Cucurbita pepo (Zucchini)cpecpiB821
CSPI02G02650Bottle gourd (USVL1VR-Ls)cpilsiB096
CSPI02G02650Melon (DHL92) v3.6.1cpimedB132
CSPI02G02650Cucumber (Gy14) v2cgybcpiB056
CSPI02G02650Silver-seed gourdcarcpiB0286
CSPI02G02650Silver-seed gourdcarcpiB1109
CSPI02G02650Cucumber (Chinese Long) v3cpicucB080
CSPI02G02650Watermelon (97103) v2cpiwmbB120
CSPI02G02650Wax gourdcpiwgoB135
CSPI02G02650Wax gourdcpiwgoB137
CSPI02G02650Cucumber (Gy14) v1cgycpiB187
CSPI02G02650Cucurbita maxima (Rimu)cmacpiB800
CSPI02G02650Cucurbita moschata (Rifu)cmocpiB159
CSPI02G02650Cucurbita moschata (Rifu)cmocpiB787
CSPI02G02650Cucumber (Chinese Long) v2cpicuB065
CSPI02G02650Melon (DHL92) v3.5.1cpimeB139
CSPI02G02650Watermelon (Charleston Gray)cpiwcgB128
CSPI02G02650Watermelon (97103) v1cpiwmB149