CSPI02G02640 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI02G02640
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionProlyl 4-hydroxylase subunit alpha-2
LocationChr2 : 1819684 .. 1822703 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGTATCTATACGCAAATACATCAAGTTACAGGGTAAGAAATGGTCCACATTTCAGCTTTCGAAGATGATCATGGCCCTCGTTTTGGCACTTGGGTTTTTCATGCTTATTGCTCTTCGCTTCTTATCTCCTCCTGAAACTTCTCACCACCGTTTCTCTTCCGTCCGGCATACAGCATTTCTAAGGTAGTCCGGTAATGCAATGGGTGTGATTCTTTAAGCTTGTTTCTTACATGAATCAACTGATTTCGATTTCCATTTTGGTAATATTGTTGTTGTTGTAGTGATGGGTTGGGGAAGAGAGGAGATCAGTGGGTTGAGTTCATTTCTTGGGAGCCTAGAGCTTTTGTATATCATAATTTCTTGGTAAGTTTCTAGAATTGTGGTTTTTTTTTTCTGGAAAAAAAAAAGGAAAATTTGTTTTGTTTTGTTAAAATGGTTGGTTGATATTGGAATGGTGTTGGACAGTCCAAGGAAGAATGCTTGTACTTGATTAGTCTTGCAAAACCTCACATGGAGAAATCAACTGTGGTTGATAACGAAACTGGCAAGAATGTGGAAGACAGGTATATATTTGTTATTTTCTTTGTTGTCCTGTTACTAACAGAAACAGAACTTGTCAATTATTGACTATGAACTAACTCTGGTGGGGCTTACACTATGACACATTTAAAAGTGTAAGAAAAAGTAATCTCAAAGAGAGTAAATTTGAACTAAACTCATATTTTAAAGATGATTTATCTATATTTAGTCCTTTCTCATAAGTTATCTCTTTAGGAATTGATTGGTCGGTTCGGTTCTTGAAAAATCTGTTCAGTAAAAGTCCAACCTGAACTGACCACAATAGAATGGTTATGTATAATAGAAGGGTATAAAGTAGAACAATACAATTACAATGAACAAGGCAAACTTATAATTTTTTGCGGTACAATGTCATGTCCACAGTGTGCGAACCAGTTCTGGGATGTTTCTGAATAGAGGGCAGGACAAAATCGTCAGTAACATAGAGAAAAGAATAGCAGATTTTACTTTCATTCCTATAGGTAAATAATTCAAAACTTTCATATCACCATGGCTAATTTCTGAAGCATTTTGTGATAATTCCTATATGTAAGAACTTATTTAGGAATACTTTTCAAAACAGTTTTTGAAAACATGTTGGAATCACCATTTTTGCTAGTTGTTTTATTTTGTCTGTGTTAGTGATTTCATTCAATACATCAATCTTTACAATGCATTCAATTGTAGTTATATTGAATAGTTCTTCAAAATAGAGTTGCAACCAGAACATAATTCTCAGACTAACCAGACATTGGCGAAAAATTAGTTAATTTCATGTGGGATTTTGTTCACCATTCTTGTCTTTTGGCCCAAACCAAAAAAGGGCTTTGGTGAATGAGCTATTCATTTGCGGCTTGTTGTTTTGTTGATGTCGCTGACATTTTTTGTCTTAATACATTTTGTTTCTGATGTTGCCATTCACAATTTGAAACAGAGCATACAGAGTATGTGGTGTAGTTGAAATGATAAACAATTATTATAATTTAGTTAATCTGGGTTTAGTTGTAAATTTTAAATTGGTTAGTGTTTGATTATTAGTTTTTTTGTTAGCCCCTTTGAGGTTTGTATTGGGAGCTCTTTATTTATTTACTTTTATTTTTAGAACTTTGTTTTAGAGACTAGAGAGTTCTCTCTCAACTCGAAGGACAAATTTCGGATTTGGTCTAATCTCAATTCTGCTTCATCAGCATGGAGAATGTGGGTTTGTTCTGCATTCTAGTTTTGAGGCCCATGGTGAACAGCATTCATTTCTCTTTGTTTTTGTTTATTTTATTTTATTTTTGTTTTACGGGTGGAGATGGTAGACCAAACCTTGACTTCTAAGAAAAGGGACGGTAGACCAAACTTTGACTTCTAAGAAAAAAGATCGTGCCAATTATCGTTGAACTAACCTCACTTTGACTATTTATTTTTTTTATTTTTTTGTTGATGGTTTTTTTATGTCTCTATCATTCTTGGTCTTATTACATTTTGGTGTGAAGAGGATTGTTTTTGTTAAAGAATAAATAAATTTTGGTACTGATATTCACAATTCCAAACAGAGCATGGAGAAGGACTTCAAATTCTCCATTATGAAGTTGGGCAGAAGTATGATGCGCATTATGATTTCTTTGATGATGAGTTCAACCTCAAAGAAATAGGCCAAAGAATGGCCACCCTCCTCATGTATTTGTAAGATTCTAATTTGACAATCTCTCAAACTCATCTAACCTCTTCCCTAGTTTCTCTCTGAATCAATACCAATACCAATGCAGGTCGGATGTTGAAGAAGGGGGCGAGACGGTGTTCCCGGCTGCGAAAGGAAACTTTAGCTCTGTGCCATGGTGGAATGAACTGTCTAAATGTGGTAAAGGTGGACTCTCTGTAAAACCAAAAATGGGAGATGCTTTATTGTTCTGGAGCATGAAGCCGGATACTACCTTAGACCCTACAAGTTTACATGGTGAGTCCTTTTCTTTTTGCTCCCTATTTTACTTCAGCATACCAAGATTGGTTACATCTAAATTATCCTCCCTATTCTTTTGTATTGATATGTAGGTGCCTGTCCTGTCATTAGAGGGAACAAATGGTCATGTACAAAGTGGATACATGTTAATCAATTAACATGAGGTGAGTTACATTTTTTTAAAAAAGTTATTCTATATACATCATTTATATATCACAAGGTGTCCAACTTGGTCGTTAAAAGAATATCATAATTAAAAAATGGACATTTTCAAATATAATTTTGTAAATATATTTTTTTCCTTACTTTGCAAAATAATTCTACCAATAAAACTTGAAATAGTATATATATATATATTATTTCCTGTAAAAGTAGATTGAGTGCTTTTTAAAAAAAAAAACTAATTTGAGTTGTATGATTTTATATGAAAATATGATTTAAAAGATAATTTGCAAATTGCAGGGTGTAATGAAAACACACTTTGTTGGGGAAGAAGACAATTGGAAGACCAC

mRNA sequence

ATGGCAGTATCTATACGCAAATACATCAAGTTACAGGGTAAGAAATGGTCCACATTTCAGCTTTCGAAGATGATCATGGCCCTCGTTTTGGCACTTGGGTTTTTCATGCTTATTGCTCTTCGCTTCTTATCTCCTCCTGAAACTTCTCACCACCGTTTCTCTTCCGTCCGGCATACAGCATTTCTAAGTGATGGGTTGGGGAAGAGAGGAGATCAGTGGGTTGAGTTCATTTCTTGGGAGCCTAGAGCTTTTGTATATCATAATTTCTTGTCCAAGGAAGAATGCTTGTACTTGATTAGTCTTGCAAAACCTCACATGGAGAAATCAACTGTGGTTGATAACGAAACTGGCAAGAATGTGGAAGACAGTGTGCGAACCAGTTCTGGGATGTTTCTGAATAGAGGGCAGGACAAAATCGTCAGTAACATAGAGAAAAGAATAGCAGATTTTACTTTCATTCCTATAGAGCATGGAGAAGGACTTCAAATTCTCCATTATGAAGTTGGGCAGAAGTATGATGCGCATTATGATTTCTTTGATGATGAGTTCAACCTCAAAGAAATAGGCCAAAGAATGGCCACCCTCCTCATGTATTTGTCGGATGTTGAAGAAGGGGGCGAGACGGTGTTCCCGGCTGCGAAAGGAAACTTTAGCTCTGTGCCATGGTGGAATGAACTGTCTAAATGTGGTAAAGGTGGACTCTCTGTAAAACCAAAAATGGGAGATGCTTTATTGTTCTGGAGCATGAAGCCGGATACTACCTTAGACCCTACAAGTTTACATGGTGCCTGTCCTGTCATTAGAGGGAACAAATGGTCATGTACAAAGTGGATACATGTTAATCAATTAACATGA

Coding sequence (CDS)

ATGGCAGTATCTATACGCAAATACATCAAGTTACAGGGTAAGAAATGGTCCACATTTCAGCTTTCGAAGATGATCATGGCCCTCGTTTTGGCACTTGGGTTTTTCATGCTTATTGCTCTTCGCTTCTTATCTCCTCCTGAAACTTCTCACCACCGTTTCTCTTCCGTCCGGCATACAGCATTTCTAAGTGATGGGTTGGGGAAGAGAGGAGATCAGTGGGTTGAGTTCATTTCTTGGGAGCCTAGAGCTTTTGTATATCATAATTTCTTGTCCAAGGAAGAATGCTTGTACTTGATTAGTCTTGCAAAACCTCACATGGAGAAATCAACTGTGGTTGATAACGAAACTGGCAAGAATGTGGAAGACAGTGTGCGAACCAGTTCTGGGATGTTTCTGAATAGAGGGCAGGACAAAATCGTCAGTAACATAGAGAAAAGAATAGCAGATTTTACTTTCATTCCTATAGAGCATGGAGAAGGACTTCAAATTCTCCATTATGAAGTTGGGCAGAAGTATGATGCGCATTATGATTTCTTTGATGATGAGTTCAACCTCAAAGAAATAGGCCAAAGAATGGCCACCCTCCTCATGTATTTGTCGGATGTTGAAGAAGGGGGCGAGACGGTGTTCCCGGCTGCGAAAGGAAACTTTAGCTCTGTGCCATGGTGGAATGAACTGTCTAAATGTGGTAAAGGTGGACTCTCTGTAAAACCAAAAATGGGAGATGCTTTATTGTTCTGGAGCATGAAGCCGGATACTACCTTAGACCCTACAAGTTTACATGGTGCCTGTCCTGTCATTAGAGGGAACAAATGGTCATGTACAAAGTGGATACATGTTAATCAATTAACATGA
Homology
[Close]
BLAST of CSPI02G02640 vs. Swiss-Prot
Match: P4H3_ARATH (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana GN=P4H3 PE=2 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 2.5e-110
Identity = 194/281 (69.04%), Postives = 222/281 (79.00%), Query Frame = 1 

Query: 7   KYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFLSPP----ETSHHRFSSVRHTAF- 66
           ++ + Q +KWST  L  + M  +L +   ML+A    S P    E+S    S  R  A  
Sbjct: 5   RHSRFQARKWSTLMLV-LFMLFMLTIVLLMLLAFGVFSLPINNDESSPIDLSYFRRAATE 64

Query: 67  LSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDNETGKNVE 126
            S+GLGKRGDQW E +SWEPRAFVYHNFLSKEEC YLISLAKPHM KSTVVD+ETGK+ +
Sbjct: 65  RSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKD 124

Query: 127 DSVRTSSGMFLNRGQDKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDFFDD 186
             VRTSSG FL RG+DKI+  IEKRIAD+TFIP +HGEGLQ+LHYE GQKY+ HYD+F D
Sbjct: 125 SRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYFVD 184

Query: 187 EFNLKEIGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSKCGKGGLSVKPKMG 246
           EFN K  GQRMAT+LMYLSDVEEGGETVFPAA  NFSSVPW+NELS+CGK GLSVKP+MG
Sbjct: 185 EFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVKPRMG 244

Query: 247 DALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNQ 283
           DALLFWSM+PD TLDPTSLHG CPVIRGNKWS TKW+HV +
Sbjct: 245 DALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGE 284

[Close]
BLAST of CSPI02G02640 vs. Swiss-Prot
Match: P4H10_ARATH (Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana GN=P4H10 PE=2 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 3.2e-102
Identity = 177/269 (65.80%), Postives = 212/269 (78.81%), Query Frame = 1 

Query: 22  SKMIMALVLALGFFMLIALRF--LSPPETS------HHRFSSVRHTAFLSDGLGKRGDQW 81
           S ++ A+++   F +LI L F  LS P  +      +   S VR T   S     + ++W
Sbjct: 18  STLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIVRKTLQRSGEDDSKNERW 77

Query: 82  VEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDNETGKNVEDSVRTSSGMFLN 141
           VE ISWEPRA VYHNFL+KEEC YLI LAKPHMEKSTVVD +TGK+ +  VRTSSG FL 
Sbjct: 78  VEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKTGKSTDSRVRTSSGTFLA 137

Query: 142 RGQDKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDFFDDEFNLKEIGQRMA 201
           RG+DK +  IEKRI+DFTFIP+EHGEGLQ+LHYE+GQKY+ HYD+F DE+N +  GQR+A
Sbjct: 138 RGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHYDYFMDEYNTRNGGQRIA 197

Query: 202 TLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSKCGKGGLSVKPKMGDALLFWSMKPDT 261
           T+LMYLSDVEEGGETVFPAAKGN+S+VPWWNELS+CGKGGLSVKPKMGDALLFWSM PD 
Sbjct: 198 TVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSVKPKMGDALLFWSMTPDA 257

Query: 262 TLDPTSLHGACPVIRGNKWSCTKWIHVNQ 283
           TLDP+SLHG C VI+GNKWS TKW+ V++
Sbjct: 258 TLDPSSLHGGCAVIKGNKWSSTKWLRVHE 286

[Close]
BLAST of CSPI02G02640 vs. Swiss-Prot
Match: P4H5_ARATH (Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana GN=P4H5 PE=2 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 2.9e-95
Identity = 166/288 (57.64%), Postives = 213/288 (73.96%), Query Frame = 1 

Query: 1   MAVSIRKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFLSPPETSHHRFSS----- 60
           MA   +++++ Q +K  +       + ++L +   +L+ L  LS P  + +   +     
Sbjct: 1   MASKSKQHLRYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTN 60

Query: 61  -VRHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDN 120
            VR +   S      G++WVE ISWEPRA VYHNFL+ EEC +LISLAKP M KSTVVD 
Sbjct: 61  IVRKSETSSGDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDE 120

Query: 121 ETGKNVEDSVRTSSGMFLNRGQDKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDA 180
           +TG + +  VRTSSG FL RG D++V  IEKRI+DFTFIP+E+GEGLQ+LHY+VGQKY+ 
Sbjct: 121 KTGGSKDSRVRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEP 180

Query: 181 HYDFFDDEFNLKEIGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSKCGKGGL 240
           HYD+F DEFN K  GQR+AT+LMYLSDV++GGETVFPAA+GN S+VPWWNELSKCGK GL
Sbjct: 181 HYDYFLDEFNTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGL 240

Query: 241 SVKPKMGDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNQ 283
           SV PK  DALLFW+M+PD +LDP+SLHG CPV++GNKWS TKW HV++
Sbjct: 241 SVLPKKRDALLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHE 288

[Close]
BLAST of CSPI02G02640 vs. Swiss-Prot
Match: P4H8_ARATH (Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana GN=P4H8 PE=3 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 5.0e-95
Identity = 166/286 (58.04%), Postives = 209/286 (73.08%), Query Frame = 1 

Query: 6   RKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFLSPPETSHHR---------FSSV 65
           +K  +L+ K   +F      + +++     +L+ L   S P T+              ++
Sbjct: 3   KKPKQLRNKPRKSFSTQTFTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQTI 62

Query: 66  RHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDNET 125
           +      D     GD+W+E ISWEPRAFVYHNFL+ EEC +LISLAKP M KS VVD +T
Sbjct: 63  QERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVKT 122

Query: 126 GKNVEDSVRTSSGMFLNRGQDKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 185
           GK+++  VRTSSG FLNRG D+IV  IE RI+DFTFIP E+GEGLQ+LHYEVGQ+Y+ H+
Sbjct: 123 GKSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPHH 182

Query: 186 DFFDDEFNLKEIGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSKCGKGGLSV 245
           D+F DEFN+++ GQR+AT+LMYLSDV+EGGETVFPAAKGN S VPWW+ELS+CGK GLSV
Sbjct: 183 DYFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLSV 242

Query: 246 KPKMGDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNQ 283
            PK  DALLFWSMKPD +LDP+SLHG CPVI+GNKWS TKW HV++
Sbjct: 243 LPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHE 288

[Close]
BLAST of CSPI02G02640 vs. Swiss-Prot
Match: P4H7_ARATH (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana GN=P4H7 PE=2 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.1e-65
Identity = 117/206 (56.80%), Postives = 154/206 (74.76%), Query Frame = 1 

Query: 77  ISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDNETGKNVEDSVRTSSGMFLNRGQ 136
           +SW PR F+Y  FLS EEC + I LAK  +EKS V DN++G++VE  VRTSSGMFL++ Q
Sbjct: 59  LSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKRQ 118

Query: 137 DKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDFFDDEFNLKEIGQRMATLL 196
           D IVSN+E ++A +TF+P E+GE +QILHYE GQKY+ H+D+F D+ NL+  G R+AT+L
Sbjct: 119 DDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATVL 178

Query: 197 MYLSDVEEGGETVFPAAKGNFSSV--PWWNELSKCGKGGLSVKPKMGDALLFWSMKPDTT 256
           MYLS+VE+GGETVFP  KG  + +    W E   C K G +VKP+ GDALLF+++ P+ T
Sbjct: 179 MYLSNVEKGGETVFPMWKGKATQLKDDSWTE---CAKQGYAVKPRKGDALLFFNLHPNAT 238

Query: 257 LDPTSLHGACPVIRGNKWSCTKWIHV 281
            D  SLHG+CPV+ G KWS T+WIHV
Sbjct: 239 TDSNSLHGSCPVVEGEKWSATRWIHV 261

[Close]
BLAST of CSPI02G02640 vs. TrEMBL
Match: A0A0A0LFF5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G009620 PE=4 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 9.3e-141
Identity = 237/257 (92.22%), Postives = 251/257 (97.67%), Query Frame = 1 

Query: 26  MALVLALGFFMLIALRFLSPPETSHHRFSSVRHTAFLSDGLGKRGDQWVEFISWEPRAFV 85
           MALVLALGFFMLIALRFLSPPETSHHRFSSVRHTAFLSDGLGKRGDQWVEFISWEPRAFV
Sbjct: 1   MALVLALGFFMLIALRFLSPPETSHHRFSSVRHTAFLSDGLGKRGDQWVEFISWEPRAFV 60

Query: 86  YHNFLSKEECLYLISLAKPHMEKSTVVDNETGKNVEDSVRTSSGMFLNRGQDKIVSNIEK 145
           YHNFLSKEECLYLISLAKPHMEKSTVVD++TG++V+  VRTSSGMFLNRGQDKI+ NIEK
Sbjct: 61  YHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLNRGQDKIIRNIEK 120

Query: 146 RIADFTFIPIEHGEGLQILHYEVGQKYDAHYDFFDDEFNLKEIGQRMATLLMYLSDVEEG 205
           RIADFTFIPIEHGEGLQILHYEVGQKYDAHYD+F DE+N+K+ GQRMATLLMYLSDVEEG
Sbjct: 121 RIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMATLLMYLSDVEEG 180

Query: 206 GETVFPAAKGNFSSVPWWNELSKCGKGGLSVKPKMGDALLFWSMKPDTTLDPTSLHGACP 265
           GETVFPAAKGNFSSVPWWNELS+CGKGGLSVKPKMGDALLFWSMKPD TLDPTSLHGACP
Sbjct: 181 GETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDATLDPTSLHGACP 240

Query: 266 VIRGNKWSCTKWIHVNQ 283
           VIRGNKWSCTKW+HV++
Sbjct: 241 VIRGNKWSCTKWMHVDK 257

[Close]
BLAST of CSPI02G02640 vs. TrEMBL
Match: A0A061FEJ2_THECC (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein isoform 1 OS=Theobroma cacao GN=TCM_034293 PE=4 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 2.0e-111
Identity = 196/285 (68.77%), Postives = 229/285 (80.35%), Query Frame = 1 

Query: 7   KYIKLQGKKWSTFQLS-------KMIMALVLALGFFMLIALRFLSPPE--TSHHRFSSVR 66
           ++ +LQ KKWST  L         +++ ++L LG F L      SPP   TS+ R +S R
Sbjct: 5   RHSRLQAKKWSTVMLVLSMLFMLTVVLLMLLGLGIFSLPMSTDDSPPNDLTSYRRMASER 64

Query: 67  HTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDNETG 126
                   LGKRG+QW E +SWEPRAF+YHNFLSKEEC YLI+LAKPHM KSTVVD++TG
Sbjct: 65  -----GKELGKRGEQWTEVLSWEPRAFIYHNFLSKEECEYLINLAKPHMAKSTVVDSKTG 124

Query: 127 KNVEDSVRTSSGMFLNRGQDKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYD 186
           ++ +  VRTSSGMFL RGQDKI+ +IEKRIAD+TFIP+EHGEGLQ+LHYEVGQKYDAH+D
Sbjct: 125 RSKDSRVRTSSGMFLRRGQDKIIRDIEKRIADYTFIPVEHGEGLQVLHYEVGQKYDAHFD 184

Query: 187 FFDDEFNLKEIGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSKCGKGGLSVK 246
           +F DEFN K  GQRMAT+LMYLSDVEEGGET+FPAAKGNFS+VPWWNELS+CGK GLSVK
Sbjct: 185 YFLDEFNTKNGGQRMATMLMYLSDVEEGGETIFPAAKGNFSAVPWWNELSECGKQGLSVK 244

Query: 247 PKMGDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNQ 283
           PKMGDALLFWSM+PD TLDP+SLHG CPVI GNKWS TKWIHV +
Sbjct: 245 PKMGDALLFWSMRPDATLDPSSLHGGCPVIMGNKWSSTKWIHVEE 284

[Close]
BLAST of CSPI02G02640 vs. TrEMBL
Match: E0CQW5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g00950 PE=4 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 3.5e-111
Identity = 195/282 (69.15%), Postives = 230/282 (81.56%), Query Frame = 1 

Query: 7   KYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFLSPP-----ETSHHRFSSVRHTAF 66
           +Y +  GK+WST  L   ++ L+L +   ML+AL  +S P       + +  SS R   F
Sbjct: 5   RYSRGHGKRWSTLALVLSLL-LMLTVVLLMLLALGIVSLPIGTVDSDAANDLSSFRRKTF 64

Query: 67  LS-DGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDNETGKNV 126
              +GLGKRG+QW E +SWEPRAF+YHNFLSKEEC Y+ISLAKP+M+KSTVVD+ETG++ 
Sbjct: 65  DGGEGLGKRGEQWTEIVSWEPRAFIYHNFLSKEECEYMISLAKPYMKKSTVVDSETGRSK 124

Query: 127 EDSVRTSSGMFLNRGQDKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDFFD 186
           +  VRTSSGMFL RG+DKI+ +IEKRIADFTFIP+EHGEGLQ+LHYEVGQKYDAHYD+F 
Sbjct: 125 DSRVRTSSGMFLRRGRDKIIRDIEKRIADFTFIPVEHGEGLQVLHYEVGQKYDAHYDYFL 184

Query: 187 DEFNLKEIGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSKCGKGGLSVKPKM 246
           DEFN K  GQR+ATLLMYLSDVEEGGETVFPA K NFSSVPWWNELS+CGK GLSVKPKM
Sbjct: 185 DEFNTKNGGQRIATLLMYLSDVEEGGETVFPATKANFSSVPWWNELSECGKKGLSVKPKM 244

Query: 247 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNQ 283
           GDALLFWSM+PD TLDP+SLHG CPVI+GNKWS TKW+HV +
Sbjct: 245 GDALLFWSMRPDATLDPSSLHGGCPVIKGNKWSSTKWMHVEE 285

[Close]
BLAST of CSPI02G02640 vs. TrEMBL
Match: M5XZ46_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009548mg PE=4 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 5.0e-110
Identity = 198/281 (70.46%), Postives = 230/281 (81.85%), Query Frame = 1 

Query: 7   KYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFLSPP----ETSHHRFSSVRH-TAF 66
           +Y +LQ KKWSTF L  + M  +L +   ML+A   +S P    E+S +  SS R  T  
Sbjct: 5   RYGRLQSKKWSTFTLV-LSMLFMLIVVLLMLLAFGIVSLPVITDESSPNDLSSFRRSTVE 64

Query: 67  LSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDNETGKNVE 126
            +DG G+R DQW E ISWEPRAF+YHNFLSKEEC YLI+LAKP M KSTVVD++TGK+ +
Sbjct: 65  RTDGFGEREDQWTEVISWEPRAFIYHNFLSKEECDYLINLAKPDMVKSTVVDSKTGKSKD 124

Query: 127 DSVRTSSGMFLNRGQDKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDFFDD 186
             VRTSSGMFL RG+DKIVS+IEKRIADFTFIP+EHGEGLQILHYEVGQKYDAH+D+F D
Sbjct: 125 SRVRTSSGMFLKRGRDKIVSDIEKRIADFTFIPVEHGEGLQILHYEVGQKYDAHFDYFLD 184

Query: 187 EFNLKEIGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSKCGKGGLSVKPKMG 246
           EFN K  GQR+ATLLMYLSDVEEGGETVFPAAKG+F+SV WW ELS+CGK GLSVKPKMG
Sbjct: 185 EFNTKNGGQRIATLLMYLSDVEEGGETVFPAAKGSFNSVRWWKELSECGKQGLSVKPKMG 244

Query: 247 DALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNQ 283
           DALLFWSM+PD +LDP+SLHG CPVIRGNKWS TKW+H+ +
Sbjct: 245 DALLFWSMRPDASLDPSSLHGGCPVIRGNKWSSTKWMHIEE 284

[Close]
BLAST of CSPI02G02640 vs. TrEMBL
Match: A0A0D2S8Z3_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G269000 PE=4 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 1.9e-109
Identity = 194/286 (67.83%), Postives = 230/286 (80.42%), Query Frame = 1 

Query: 7   KYIKLQGKKWST--------FQLSKMIMALVLALGFFMLIALRFLSPPE--TSHHRFSSV 66
           ++ +LQ +KWST        F LS +++ ++L LG F L      S P   TS+ R +S 
Sbjct: 5   RHSRLQARKWSTVTLVLSMLFMLS-VVLLMLLGLGVFFLPINDDDSAPNDLTSYRRMASE 64

Query: 67  RHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDNET 126
           R       GLGKRG+QW E +SWEPRAF+YHNFLSKEEC YLI+LAKPHM KSTVVD++T
Sbjct: 65  R-----GKGLGKRGEQWTEVLSWEPRAFIYHNFLSKEECEYLINLAKPHMVKSTVVDSKT 124

Query: 127 GKNVEDSVRTSSGMFLNRGQDKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 186
           GK+ +  VRTSSGMFL RGQDKI+ +IEKRIAD++FIP+EHGEGLQ+LHYEVGQKYDAH+
Sbjct: 125 GKSKDSRVRTSSGMFLRRGQDKIIKDIEKRIADYSFIPVEHGEGLQVLHYEVGQKYDAHF 184

Query: 187 DFFDDEFNLKEIGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSKCGKGGLSV 246
           D+F DEFN K  GQRMAT+LMYLSDVEEGGET+FPAAKGN SSVPWWNELS+CGK GL+V
Sbjct: 185 DYFLDEFNTKNGGQRMATMLMYLSDVEEGGETIFPAAKGNISSVPWWNELSECGKQGLAV 244

Query: 247 KPKMGDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNQ 283
           KPKMGDALLFWSM+PD TLDP+SLHG CPVI GNKWS TKW+H+ +
Sbjct: 245 KPKMGDALLFWSMRPDATLDPSSLHGGCPVIMGNKWSSTKWMHLEE 284

[Close]
BLAST of CSPI02G02640 vs. TAIR10
Match: AT1G20270.1 (AT1G20270.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 399.8 bits (1026), Expect = 1.4e-111
Identity = 194/281 (69.04%), Postives = 222/281 (79.00%), Query Frame = 1 

Query: 7   KYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFLSPP----ETSHHRFSSVRHTAF- 66
           ++ + Q +KWST  L  + M  +L +   ML+A    S P    E+S    S  R  A  
Sbjct: 5   RHSRFQARKWSTLMLV-LFMLFMLTIVLLMLLAFGVFSLPINNDESSPIDLSYFRRAATE 64

Query: 67  LSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDNETGKNVE 126
            S+GLGKRGDQW E +SWEPRAFVYHNFLSKEEC YLISLAKPHM KSTVVD+ETGK+ +
Sbjct: 65  RSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKD 124

Query: 127 DSVRTSSGMFLNRGQDKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDFFDD 186
             VRTSSG FL RG+DKI+  IEKRIAD+TFIP +HGEGLQ+LHYE GQKY+ HYD+F D
Sbjct: 125 SRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYFVD 184

Query: 187 EFNLKEIGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSKCGKGGLSVKPKMG 246
           EFN K  GQRMAT+LMYLSDVEEGGETVFPAA  NFSSVPW+NELS+CGK GLSVKP+MG
Sbjct: 185 EFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVKPRMG 244

Query: 247 DALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNQ 283
           DALLFWSM+PD TLDPTSLHG CPVIRGNKWS TKW+HV +
Sbjct: 245 DALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGE 284

[Close]
BLAST of CSPI02G02640 vs. TAIR10
Match: AT5G66060.1 (AT5G66060.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 372.9 bits (956), Expect = 1.8e-103
Identity = 177/269 (65.80%), Postives = 212/269 (78.81%), Query Frame = 1 

Query: 22  SKMIMALVLALGFFMLIALRF--LSPPETS------HHRFSSVRHTAFLSDGLGKRGDQW 81
           S ++ A+++   F +LI L F  LS P  +      +   S VR T   S     + ++W
Sbjct: 18  STLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIVRKTLQRSGEDDSKNERW 77

Query: 82  VEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDNETGKNVEDSVRTSSGMFLN 141
           VE ISWEPRA VYHNFL+KEEC YLI LAKPHMEKSTVVD +TGK+ +  VRTSSG FL 
Sbjct: 78  VEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKTGKSTDSRVRTSSGTFLA 137

Query: 142 RGQDKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDFFDDEFNLKEIGQRMA 201
           RG+DK +  IEKRI+DFTFIP+EHGEGLQ+LHYE+GQKY+ HYD+F DE+N +  GQR+A
Sbjct: 138 RGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHYDYFMDEYNTRNGGQRIA 197

Query: 202 TLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSKCGKGGLSVKPKMGDALLFWSMKPDT 261
           T+LMYLSDVEEGGETVFPAAKGN+S+VPWWNELS+CGKGGLSVKPKMGDALLFWSM PD 
Sbjct: 198 TVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSVKPKMGDALLFWSMTPDA 257

Query: 262 TLDPTSLHGACPVIRGNKWSCTKWIHVNQ 283
           TLDP+SLHG C VI+GNKWS TKW+ V++
Sbjct: 258 TLDPSSLHGGCAVIKGNKWSSTKWLRVHE 286

[Close]
BLAST of CSPI02G02640 vs. TAIR10
Match: AT2G17720.1 (AT2G17720.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 349.7 bits (896), Expect = 1.6e-96
Identity = 166/288 (57.64%), Postives = 213/288 (73.96%), Query Frame = 1 

Query: 1   MAVSIRKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFLSPPETSHHRFSS----- 60
           MA   +++++ Q +K  +       + ++L +   +L+ L  LS P  + +   +     
Sbjct: 1   MASKSKQHLRYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTN 60

Query: 61  -VRHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDN 120
            VR +   S      G++WVE ISWEPRA VYHNFL+ EEC +LISLAKP M KSTVVD 
Sbjct: 61  IVRKSETSSGDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDE 120

Query: 121 ETGKNVEDSVRTSSGMFLNRGQDKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDA 180
           +TG + +  VRTSSG FL RG D++V  IEKRI+DFTFIP+E+GEGLQ+LHY+VGQKY+ 
Sbjct: 121 KTGGSKDSRVRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEP 180

Query: 181 HYDFFDDEFNLKEIGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSKCGKGGL 240
           HYD+F DEFN K  GQR+AT+LMYLSDV++GGETVFPAA+GN S+VPWWNELSKCGK GL
Sbjct: 181 HYDYFLDEFNTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGL 240

Query: 241 SVKPKMGDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNQ 283
           SV PK  DALLFW+M+PD +LDP+SLHG CPV++GNKWS TKW HV++
Sbjct: 241 SVLPKKRDALLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHE 288

[Close]
BLAST of CSPI02G02640 vs. TAIR10
Match: AT4G35810.1 (AT4G35810.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 349.0 bits (894), Expect = 2.8e-96
Identity = 166/286 (58.04%), Postives = 209/286 (73.08%), Query Frame = 1 

Query: 6   RKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFLSPPETSHHR---------FSSV 65
           +K  +L+ K   +F      + +++     +L+ L   S P T+              ++
Sbjct: 3   KKPKQLRNKPRKSFSTQTFTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQTI 62

Query: 66  RHTAFLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDNET 125
           +      D     GD+W+E ISWEPRAFVYHNFL+ EEC +LISLAKP M KS VVD +T
Sbjct: 63  QERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVKT 122

Query: 126 GKNVEDSVRTSSGMFLNRGQDKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHY 185
           GK+++  VRTSSG FLNRG D+IV  IE RI+DFTFIP E+GEGLQ+LHYEVGQ+Y+ H+
Sbjct: 123 GKSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPHH 182

Query: 186 DFFDDEFNLKEIGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSKCGKGGLSV 245
           D+F DEFN+++ GQR+AT+LMYLSDV+EGGETVFPAAKGN S VPWW+ELS+CGK GLSV
Sbjct: 183 DYFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLSV 242

Query: 246 KPKMGDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNQ 283
            PK  DALLFWSMKPD +LDP+SLHG CPVI+GNKWS TKW HV++
Sbjct: 243 LPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHE 288

[Close]
BLAST of CSPI02G02640 vs. TAIR10
Match: AT5G18900.1 (AT5G18900.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 240.7 bits (613), Expect = 1.1e-63
Identity = 114/210 (54.29%), Postives = 156/210 (74.29%), Query Frame = 1 

Query: 74  VEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDNETGKNVEDSVRTSSGMFLN 133
           V+ +S +PRAFVY  FL++ EC +++SLAK  +++S V DN++G++    VRTSSG F++
Sbjct: 37  VKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGESKFSEVRTSSGTFIS 96

Query: 134 RGQDKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDFFDDEFNLKEIGQRMA 193
           +G+D IVS IE +I+ +TF+P E+GE +Q+L YE GQKYDAH+D+F D+ N+   G RMA
Sbjct: 97  KGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMA 156

Query: 194 TLLMYLSDVEEGGETVFPAAKGNFSSVPWWN--ELSKCGKGGLSVKPKMGDALLFWSMKP 253
           T+LMYLS+V +GGETVFP A+     V   N  +LS C K G++VKP+ GDALLF+++ P
Sbjct: 157 TILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKRGIAVKPRKGDALLFFNLHP 216

Query: 254 DTTLDPTSLHGACPVIRGNKWSCTKWIHVN 282
           D   DP SLHG CPVI G KWS TKWIHV+
Sbjct: 217 DAIPDPLSLHGGCPVIEGEKWSATKWIHVD 246

[Close]
BLAST of CSPI02G02640 vs. NCBI nr
Match: gi|659070723|ref|XP_008456352.1| (PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like [Cucumis melo])

HSP 1 Score: 550.4 bits (1417), Expect = 1.8e-153
Identity = 261/282 (92.55%), Postives = 271/282 (96.10%), Query Frame = 1 

Query: 1   MAVSIRKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFLSPPETSHHRFSSVRHTA 60
           MAVSI KYIKLQGKKWSTFQLSKMIMALVLALGFFML AL F SPPETSHHR SSVRHTA
Sbjct: 1   MAVSIGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLSALWFFSPPETSHHRLSSVRHTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDNETGKNV 120
           FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVD+ETGK+V
Sbjct: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSETGKSV 120

Query: 121 EDSVRTSSGMFLNRGQDKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDFFD 180
           + SVRTSSGMFLNRGQDKI+SNIEKRIADFTFIPIEHGE +QILHY VGQKYDAHYDFF 
Sbjct: 121 DSSVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEDIQILHYAVGQKYDAHYDFFV 180

Query: 181 DEFNLKEIGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSKCGKGGLSVKPKM 240
           DE+NLK +GQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELS+CGK GLS+KPKM
Sbjct: 181 DEYNLKSVGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKSGLSIKPKM 240

Query: 241 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNQ 283
           GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVN+
Sbjct: 241 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNK 282

[Close]
BLAST of CSPI02G02640 vs. NCBI nr
Match: gi|778666404|ref|XP_011648735.1| (PREDICTED: probable prolyl 4-hydroxylase 3 [Cucumis sativus])

HSP 1 Score: 547.7 bits (1410), Expect = 1.2e-152
Identity = 258/282 (91.49%), Postives = 274/282 (97.16%), Query Frame = 1 

Query: 1   MAVSIRKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFLSPPETSHHRFSSVRHTA 60
           MA+S  KYIKLQG+KWSTFQLSKMIMALVLALGFFMLIALRFLSPPETSHHRFSSVRHTA
Sbjct: 1   MAISKGKYIKLQGRKWSTFQLSKMIMALVLALGFFMLIALRFLSPPETSHHRFSSVRHTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDNETGKNV 120
           FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVD++TG++V
Sbjct: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESV 120

Query: 121 EDSVRTSSGMFLNRGQDKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDFFD 180
           +  VRTSSGMFLNRGQDKI+ NIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYD+F 
Sbjct: 121 DSRVRTSSGMFLNRGQDKIIRNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180

Query: 181 DEFNLKEIGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSKCGKGGLSVKPKM 240
           DE+N+K+ GQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELS+CGKGGLSVKPKM
Sbjct: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240

Query: 241 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNQ 283
           GDALLFWSMKPD TLDPTSLHGACPVIRGNKWSCTKW+HV++
Sbjct: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVDK 282

[Close]
BLAST of CSPI02G02640 vs. NCBI nr
Match: gi|659070731|ref|XP_008456388.1| (PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like isoform X2 [Cucumis melo])

HSP 1 Score: 543.5 bits (1399), Expect = 2.2e-151
Identity = 258/282 (91.49%), Postives = 270/282 (95.74%), Query Frame = 1 

Query: 1   MAVSIRKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFLSPPETSHHRFSSVRHTA 60
           MAVS  KYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRF SPPETSHHR  SVR TA
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRLPSVRRTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDNETGKNV 120
           F SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVD++TGK+V
Sbjct: 61  FQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGKSV 120

Query: 121 EDSVRTSSGMFLNRGQDKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDFFD 180
           +  VRTSSGMFLNRGQDKI+SNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYD+F 
Sbjct: 121 DSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180

Query: 181 DEFNLKEIGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSKCGKGGLSVKPKM 240
           DE+N+K+ GQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELS+CGKGGLSVKPKM
Sbjct: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240

Query: 241 GDALLFWSMKPDTTLDPTSLHGACPVIRGNKWSCTKWIHVNQ 283
           GDALLFWSMKPD TLDPTSLHGACPVIRGNKWSCTKW+HVN+
Sbjct: 241 GDALLFWSMKPDATLDPTSLHGACPVIRGNKWSCTKWMHVNK 282

[Close]
BLAST of CSPI02G02640 vs. NCBI nr
Match: gi|700205656|gb|KGN60775.1| (hypothetical protein Csa_2G009620 [Cucumis sativus])

HSP 1 Score: 507.7 bits (1306), Expect = 1.3e-140
Identity = 237/257 (92.22%), Postives = 251/257 (97.67%), Query Frame = 1 

Query: 26  MALVLALGFFMLIALRFLSPPETSHHRFSSVRHTAFLSDGLGKRGDQWVEFISWEPRAFV 85
           MALVLALGFFMLIALRFLSPPETSHHRFSSVRHTAFLSDGLGKRGDQWVEFISWEPRAFV
Sbjct: 1   MALVLALGFFMLIALRFLSPPETSHHRFSSVRHTAFLSDGLGKRGDQWVEFISWEPRAFV 60

Query: 86  YHNFLSKEECLYLISLAKPHMEKSTVVDNETGKNVEDSVRTSSGMFLNRGQDKIVSNIEK 145
           YHNFLSKEECLYLISLAKPHMEKSTVVD++TG++V+  VRTSSGMFLNRGQDKI+ NIEK
Sbjct: 61  YHNFLSKEECLYLISLAKPHMEKSTVVDSKTGESVDSRVRTSSGMFLNRGQDKIIRNIEK 120

Query: 146 RIADFTFIPIEHGEGLQILHYEVGQKYDAHYDFFDDEFNLKEIGQRMATLLMYLSDVEEG 205
           RIADFTFIPIEHGEGLQILHYEVGQKYDAHYD+F DE+N+K+ GQRMATLLMYLSDVEEG
Sbjct: 121 RIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFVDEYNIKKGGQRMATLLMYLSDVEEG 180

Query: 206 GETVFPAAKGNFSSVPWWNELSKCGKGGLSVKPKMGDALLFWSMKPDTTLDPTSLHGACP 265
           GETVFPAAKGNFSSVPWWNELS+CGKGGLSVKPKMGDALLFWSMKPD TLDPTSLHGACP
Sbjct: 181 GETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKMGDALLFWSMKPDATLDPTSLHGACP 240

Query: 266 VIRGNKWSCTKWIHVNQ 283
           VIRGNKWSCTKW+HV++
Sbjct: 241 VIRGNKWSCTKWMHVDK 257

[Close]
BLAST of CSPI02G02640 vs. NCBI nr
Match: gi|659070729|ref|XP_008456383.1| (PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like isoform X1 [Cucumis melo])

HSP 1 Score: 497.3 bits (1279), Expect = 1.8e-137
Identity = 240/262 (91.60%), Postives = 250/262 (95.42%), Query Frame = 1 

Query: 1   MAVSIRKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFLSPPETSHHRFSSVRHTA 60
           MAVS  KYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRF SPPETSHHR  SVR TA
Sbjct: 1   MAVSKGKYIKLQGKKWSTFQLSKMIMALVLALGFFMLIALRFFSPPETSHHRLPSVRRTA 60

Query: 61  FLSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDNETGKNV 120
           F SDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVD++TGK+V
Sbjct: 61  FQSDGLGKRGDQWVEFISWEPRAFVYHNFLSKEECLYLISLAKPHMEKSTVVDSKTGKSV 120

Query: 121 EDSVRTSSGMFLNRGQDKIVSNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDFFD 180
           +  VRTSSGMFLNRGQDKI+SNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYD+F 
Sbjct: 121 DSRVRTSSGMFLNRGQDKIISNIEKRIADFTFIPIEHGEGLQILHYEVGQKYDAHYDYFV 180

Query: 181 DEFNLKEIGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSKCGKGGLSVKPKM 240
           DE+N+K+ GQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELS+CGKGGLSVKPKM
Sbjct: 181 DEYNIKKGGQRMATLLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSECGKGGLSVKPKM 240

Query: 241 GDALLFWSMKPDTTLDPTSLHG 263
           GDALLFWSMKPD TLDPTSLHG
Sbjct: 241 GDALLFWSMKPDATLDPTSLHG 262

The following BLAST results are available for this feature:
BLAST of CSPI02G02640 vs. Swiss-Prot
Match NameE-valueIdentityDescription
P4H3_ARATH2.5e-11069.04Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana GN=P4H3 PE=2 SV=1[more]
P4H10_ARATH3.2e-10265.80Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana GN=P4H10 PE=2 SV=1[more]
P4H5_ARATH2.9e-9557.64Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana GN=P4H5 PE=2 SV=1[more]
P4H8_ARATH5.0e-9558.04Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana GN=P4H8 PE=3 SV=1[more]
P4H7_ARATH1.1e-6556.80Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana GN=P4H7 PE=2 SV=1[more]
BLAST of CSPI02G02640 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LFF5_CUCSA9.3e-14192.22Uncharacterized protein OS=Cucumis sativus GN=Csa_2G009620 PE=4 SV=1[more]
A0A061FEJ2_THECC2.0e-11168.772-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein isoform ... [more]
E0CQW5_VITVI3.5e-11169.15Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g00950 PE=4 SV=... [more]
M5XZ46_PRUPE5.0e-11070.46Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009548mg PE=4 SV=1[more]
A0A0D2S8Z3_GOSRA1.9e-10967.83Uncharacterized protein OS=Gossypium raimondii GN=B456_009G269000 PE=4 SV=1[more]
BLAST of CSPI02G02640 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G20270.11.4e-11169.04 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT5G66060.11.8e-10365.80 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT2G17720.11.6e-9657.64 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT4G35810.12.8e-9658.04 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT5G18900.11.1e-6354.29 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
BLAST of CSPI02G02640 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659070723|ref|XP_008456352.1|1.8e-15392.55PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like [Cucumis melo][more]
gi|778666404|ref|XP_011648735.1|1.2e-15291.49PREDICTED: probable prolyl 4-hydroxylase 3 [Cucumis sativus][more]
gi|659070731|ref|XP_008456388.1|2.2e-15191.49PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like isoform X2 [Cucumis melo][more]
gi|700205656|gb|KGN60775.1|1.3e-14092.22hypothetical protein Csa_2G009620 [Cucumis sativus][more]
gi|659070729|ref|XP_008456383.1|1.8e-13791.60PREDICTED: prolyl 4-hydroxylase subunit alpha-1-like isoform X1 [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR006620Pro_4_hyd_alph
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506iron ion binding
GO:0031418L-ascorbic acid binding
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005768 endosome
cellular_component GO:0005802 trans-Golgi network
molecular_function GO:0005506 iron ion binding
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G02640.1CSPI02G02640.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF136402OG-FeII_Oxy_3coord: 161..279
score: 1.1
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 157..280
score: 12
IPR006620Prolyl 4-hydroxylase, alpha subunitSMARTSM00702p4hccoord: 81..279
score: 6.0
NoneNo IPR availablePANTHERPTHR10869PROLYL 4-HYDROXYLASE ALPHA SUBUNITcoord: 12..281
score: 3.3E
NoneNo IPR availablePANTHERPTHR10869:SF782-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEINcoord: 12..281
score: 3.3E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI02G02640Cla013627Watermelon (97103) v1cpiwmB149
CSPI02G02640MELO3C004414Melon (DHL92) v3.5.1cpimeB139
CSPI02G02640ClCG02G016950Watermelon (Charleston Gray)cpiwcgB128
CSPI02G02640CsaV3_2G003820Cucumber (Chinese Long) v3cpicucB080
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CSPI02G02640Cucumber (Gy14) v2cgybcpiB056
CSPI02G02640Silver-seed gourdcarcpiB0286
CSPI02G02640Silver-seed gourdcarcpiB1109
CSPI02G02640Watermelon (97103) v2cpiwmbB120
CSPI02G02640Wax gourdcpiwgoB135
CSPI02G02640Wax gourdcpiwgoB137
CSPI02G02640Cucumber (Gy14) v1cgycpiB187
CSPI02G02640Cucurbita maxima (Rimu)cmacpiB174
CSPI02G02640Cucurbita maxima (Rimu)cmacpiB800
CSPI02G02640Cucurbita moschata (Rifu)cmocpiB159
CSPI02G02640Cucurbita moschata (Rifu)cmocpiB787
CSPI02G02640Cucumber (Chinese Long) v2cpicuB065
CSPI02G02640Cucurbita pepo (Zucchini)cpecpiB100
CSPI02G02640Cucurbita pepo (Zucchini)cpecpiB821
CSPI02G02640Bottle gourd (USVL1VR-Ls)cpilsiB096
CSPI02G02640Melon (DHL92) v3.6.1cpimedB132