CSPI01G33450 (gene) Wild cucumber (PI 183967)

BLAST of CSPI01G33450 vs. Swiss-Prot
Match: PGLR_PRUPE (Polygalacturonase OS=Prunus persica PE=2 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 4.1e-116
Identity = 207/384 (53.91%), Postives = 270/384 (70.31%), Query Frame = 1

Query: 7   SLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRSTINVPKG 66
           SL  I  F +   I++   YNV S GAK DGKTDST++FL AW  AC+S     I VP G
Sbjct: 9   SLSLIFVFMINSAIASPLTYNVASLGAKADGKTDSTKAFLSAWAKACASMNPGVIYVPAG 68

Query: 67  RFLLTPITFRGPCKNK-ITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGGSIDGK 126
            F L  + F GPCKN  ITF + GTLVAP DYR +G++  WI F  V+GV+  GG +DG+
Sbjct: 69  TFFLRDVVFSGPCKNNAITFRIAGTLVAPSDYRVIGNAANWIFFHHVNGVTISGGILDGQ 128

Query: 127 GTAYWACKNS-AKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVVKNVKI 186
           GTA WACK    ++CP GA ++ F+ +NNI++SGL S+NSQ  H+VIN   NV ++ V++
Sbjct: 129 GTALWACKACHGESCPSGATTLGFSDSNNIVVSGLASLNSQMFHIVINDFQNVQMQGVRV 188

Query: 187 MAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMRDIKCGPGHGVSIGS 246
                SPNTDGIHVQ S+ VTI  S I TGDDC+SIG GT NL++  + CGPGHG+SIGS
Sbjct: 189 SRSGNSPNTDGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLWIEGVACGPGHGISIGS 248

Query: 247 LGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNPILID 306
           LGKE  E GV+NVT+    F+ + NG+RIK+W  PS GF R+++FQ+  M+NV+NPI+ID
Sbjct: 249 LGKEQEEAGVQNVTVKTVTFSGTQNGLRIKSWGRPSTGFARNILFQHATMVNVENPIVID 308

Query: 307 QNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLEDIKLTYK 366
           Q+YCP+++ C  Q SGV+I+DVTY+ I GTSAT  AV FDCS   PC +IKLED+KLTYK
Sbjct: 309 QHYCPDNKGCPGQVSGVQISDVTYEDIHGTSATEVAVKFDCSPKHPCREIKLEDVKLTYK 368

Query: 367 NKTTTSSCKNIGGSSIGLVVPETC 389
           N+   SSC +  G++ G+V P +C
Sbjct: 369 NQAAESSCSHADGTTEGVVQPTSC 392

BLAST of CSPI01G33450 vs. Swiss-Prot
Match: PGLR6_ARATH (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 3.7e-101
Identity = 189/365 (51.78%), Postives = 254/365 (69.59%), Query Frame = 1

Query: 27  NVISFGAKPDGKTDSTQSFLKAWTSACSSSTRSTINVPKGRFLLTPITFRG-PCKNK-IT 86
           NV+S+GAKPDG  DST++FL AW  AC+S+  +TI VPKGRFL+  + F G  CK   I+
Sbjct: 34  NVLSYGAKPDGSKDSTKAFLAAWDVACASANPTTIIVPKGRFLVGNLVFHGNECKQAPIS 93

Query: 87  FLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGGSIDGKGTAYWACKNSA-KNCPPGA 146
             + G++VAP D+R +  S +WI F  V  VS  GG +D +GT+ W CKN+   NCP GA
Sbjct: 94  IRIAGSIVAPEDFRIIASSKHWIWFEDVTDVSIYGGILDAQGTSLWKCKNNGGHNCPTGA 153

Query: 147 RSITFNWANNIILSGLTSINSQQTHVVINSCNNVVVKNVKIMAPDQSPNTDGIHVQSSTN 206
           +S+ F+ +NNI +SGLTSINSQ+ H+VI++ NNV +  VK+ A + SPNTDGIHV+SS +
Sbjct: 154 KSLVFSGSNNIKISGLTSINSQKFHIVIDNSNNVNIDGVKVSADENSPNTDGIHVESSHS 213

Query: 207 VTITGSTIKTGDDCISIGDGTKNLFMRDIKCGPGHGVSIGSLGKEANENGVENVTLINAV 266
           V IT S I TGDDCISIG G+ N+F++ I+CGPGHG+SIGSLG+   E GV+NVT+ N  
Sbjct: 214 VHITNSRIGTGDDCISIGPGSTNVFIQTIRCGPGHGISIGSLGRAEEEQGVDNVTVSNVD 273

Query: 267 FTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNPILIDQNYCPNHQSCSLQSSGVKI 326
           F  ++NGVRIKTW   SN F R+++FQ+I M  VKNPI+IDQ+YC  H+ C  Q SGVK+
Sbjct: 274 FMGTNNGVRIKTWGKDSNSFARNIVFQHINMKMVKNPIIIDQHYC-LHKPCPKQESGVKV 333

Query: 327 NDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLEDIKLTYKNKTTTSSCKNIGGSSIGLV 386
           ++V Y+ I GTS T  AV  DCS   PC+ I ++D+ L   ++   +SC N  GS+  +V
Sbjct: 334 SNVRYEDIHGTSNTEVAVLLDCSKEKPCTGIVMDDVNLVSVHRPAQASCDNANGSANDVV 393

Query: 387 VPETC 389
               C
Sbjct: 394 PFTPC 397

BLAST of CSPI01G33450 vs. Swiss-Prot
Match: PGLR_GOSBA (Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 4.1e-84
Identity = 164/366 (44.81%), Postives = 232/366 (63.39%), Query Frame = 1

Query: 28  VISFGAKPDGKTDSTQSFLKAWTSACSSSTRSTINVPKGRFLLTPITFRGPCKNKITFLL 87
           V  FGAK DGKTD ++ FL AW  AC+S T ST+ +PKG +LL+ +   GPCK  I   +
Sbjct: 32  VAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCKAPIEINV 91

Query: 88  NGTLVAPLDYRALGDSGYWILFIKVDGVSFVGGSI-DGKGTAYWACKNSAKNCPPGAR-- 147
            GT+ AP D  A  D   W+ F  V+     GG I DG+G+  +  KN+ +N    ++  
Sbjct: 92  QGTIQAPADPSAFKDPN-WVRFYSVENFKMFGGGIFDGQGSIAYE-KNTCENREFRSKLP 151

Query: 148 -SITFNWANNIILSGLTSINSQQTHVVINSCNNVVVKNVKIMAPDQSPNTDGIHVQSSTN 207
            +I F++  N ++  +TS +S+  H+ + +C N+ ++ +KI APD+SPNTDGIH+  S  
Sbjct: 152 VNIRFDFVTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEG 211

Query: 208 VTITGSTIKTGDDCISIGDGTKNLFMRDIKCGPGHGVSIGSLGKEANENGVENVTLINAV 267
           V I  S IKTGDDCISIGDGTKN+ +++I CGPGHG+SIGSLGK  NE  VE + + N  
Sbjct: 212 VNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCT 271

Query: 268 FTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNPILIDQNYCPNHQSCSLQSSGVKI 327
            T + NG RIKTWP    G V  + F++I M NV +PILIDQ YCP ++    + S VK+
Sbjct: 272 ITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKL 331

Query: 328 NDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLEDIKLTYKN-KTTTSSCKNIGGSSIGL 387
           +++++K+I GTSA  EA+ F CS SSPC +++L DI + +   +  TS C N+   +IG 
Sbjct: 332 SNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIQHNGAEPATSQCLNVKPITIGK 391

Query: 388 VVPETC 389
           + P  C
Sbjct: 392 LNPIPC 395

BLAST of CSPI01G33450 vs. Swiss-Prot
Match: PGLR_GOSHI (Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 6.0e-83
Identity = 166/385 (43.12%), Postives = 239/385 (62.08%), Query Frame = 1

Query: 9   VFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRSTINVPKGRF 68
           V ++ F    ++ + A   V  FGAK DGKTD ++ FL AW  AC+S T ST+ +PKG +
Sbjct: 13  VLLLLFISASKVQSDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKGTY 72

Query: 69  LLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGGSI-DGKGT 128
           LL+ +   GPCK  I   + GT+ AP D  A  D   W+ F  V+     GG I DG+G+
Sbjct: 73  LLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPN-WVRFYSVENFKMFGGGIFDGQGS 132

Query: 129 AYWACKNSAKNCPPGAR---SITFNWANNIILSGLTSINSQQTHVVINSCNNVVVKNVKI 188
             +  KN+ +N    ++   +I F++  N ++  +TS +S+  H+ + +C N+ ++ +KI
Sbjct: 133 IAYE-KNTCENREFRSKLPVNIRFDFLTNALIQDITSKDSKLFHINVFACKNITLERLKI 192

Query: 189 MAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMRDIKCGPGHGVSIGS 248
            APD+SPNTDGIH+  S  V I  S IKTGDDCISIGDGTKN+ +++I CGPGHG+SIGS
Sbjct: 193 EAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGS 252

Query: 249 LGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNPILID 308
           LGK  NE  VE + + N   T + NG RIKTWP    G V  + F++I M NV +PILID
Sbjct: 253 LGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILID 312

Query: 309 QNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLEDIKLTYK 368
           Q YCP ++    + S VK++++++K+I GTSA  EA+ F CS SSPC +++L DI + + 
Sbjct: 313 QQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIKHN 372

Query: 369 N-KTTTSSCKNIGGSSIGLVVPETC 389
             +  TS C N+   + G + P  C
Sbjct: 373 GAEPATSQCLNVKPITSGKLNPIPC 395

BLAST of CSPI01G33450 vs. Swiss-Prot
Match: ADPG1_ARATH (Polygalacturonase ADPG1 OS=Arabidopsis thaliana GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 6.2e-80
Identity = 168/371 (45.28%), Postives = 232/371 (62.53%), Query Frame = 1

Query: 23  AANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRSTINVPKGR-FLLTPITFRGPCKN 82
           A+  +V +FGAK DGKTD TQ+F KAW  ACS++  +T  VPKG+ +LL    FRGPCK+
Sbjct: 65  ASTVSVSNFGAKGDGKTDDTQAFKKAWKKACSTNGVTTFLVPKGKTYLLKSTRFRGPCKS 124

Query: 83  KITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGGS---IDGKGTAYW--ACK-NS 142
              F + GTL A        D  +W++   V+ +S  GGS   I+G G  +W  +CK + 
Sbjct: 125 LRNFQILGTLSASTKRSDYKDKNHWLILEDVNNLSIDGGSTGIINGNGKTWWQNSCKIDK 184

Query: 143 AKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVVKNVKIMAPDQSPNTDG 202
           +K C     ++T     N+ +  L   N+QQ  + I  CN V V NV+I AP  SPNTDG
Sbjct: 185 SKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSNVEITAPGDSPNTDG 244

Query: 203 IHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMRDIKCGPGHGVSIGSLGKEANENGVE 262
           IH+ ++ N+ ++ S I TGDDCISI DGT+NL + D+ CGPGHG+SIGSLG + ++  V 
Sbjct: 245 IHITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGISIGSLGDDNSKAYVS 304

Query: 263 NVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNPILIDQNYCPNHQSCS 322
            + +  A F++SDNGVRIKT+   S G  +++ FQNI M NVKNPI+IDQ+YC +   C 
Sbjct: 305 GINVDGAKFSESDNGVRIKTYQGGS-GTAKNIKFQNIRMENVKNPIIIDQDYC-DKDKCE 364

Query: 323 LQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLEDIKLTYKNKTTTSSCKNI 382
            Q S V++ +V YK+I GTSAT  A+T +CS   PC  I LE++K+    K  T+SCKN 
Sbjct: 365 DQESAVQVKNVVYKNISGTSATDVAITLNCSEKYPCQGIVLENVKI----KGGTASCKNA 424

Query: 383 GGSSIGLVVPE 387
              + G V P+
Sbjct: 425 NVKNQGTVSPK 429

BLAST of CSPI01G33450 vs. TrEMBL
Match: A0A0A0M0F3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G689640 PE=3 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 5.1e-222
Identity = 386/389 (99.23%), Postives = 388/389 (99.74%), Query Frame = 1

Query: 1   MANPTKSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRST 60
           MANPTKSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRST
Sbjct: 1   MANPTKSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRST 60

Query: 61  INVPKGRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGG 120
           INVPKGRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGG
Sbjct: 61  INVPKGRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGG 120

Query: 121 SIDGKGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVVK 180
           +IDGKGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVVK
Sbjct: 121 TIDGKGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVVK 180

Query: 181 NVKIMAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMRDIKCGPGHGV 240
           NVKIMAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFM DIKCGPGHGV
Sbjct: 181 NVKIMAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMSDIKCGPGHGV 240

Query: 241 SIGSLGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNP 300
           SIGSLGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNP
Sbjct: 241 SIGSLGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNP 300

Query: 301 ILIDQNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLEDIK 360
           ILIDQNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSS+PCSDIKLEDIK
Sbjct: 301 ILIDQNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSNPCSDIKLEDIK 360

Query: 361 LTYKNKTTTSSCKNIGGSSIGLVVPETCF 390
           LTYKNKTTTSSCKNIGGSSIGLVVPETCF
Sbjct: 361 LTYKNKTTTSSCKNIGGSSIGLVVPETCF 389

BLAST of CSPI01G33450 vs. TrEMBL
Match: A0A067E8S7_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g037377mg PE=3 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 4.2e-160
Identity = 265/383 (69.19%), Postives = 330/383 (86.16%), Query Frame = 1

Query: 6   KSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRSTINVPK 65
           K ++F++F  +FI+ S AANYNVI++GAKPDG+TDST+ FLKAW SAC S+  STI VPK
Sbjct: 4   KLIIFLLFVIIFIKSSNAANYNVITYGAKPDGRTDSTKPFLKAWASACRSAKASTIYVPK 63

Query: 66  GRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGGSIDGK 125
           GR+L+    FRGPC+N++T  ++GT+VAP +Y ALG+SGYWILFIK+D +S +GG+ DGK
Sbjct: 64  GRYLIKAAEFRGPCRNRVTLQIDGTIVAPTNYWALGNSGYWILFIKIDRLSVIGGTFDGK 123

Query: 126 GTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVVKNVKIM 185
           G  +WAC+ S KNCP GA SITFNWANNI++SGLTSINSQQTH+VINSCNNV V+NVK++
Sbjct: 124 GAGFWACRKSGKNCPAGASSITFNWANNILISGLTSINSQQTHLVINSCNNVNVRNVKVV 183

Query: 186 APDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMRDIKCGPGHGVSIGSL 245
           APDQSPNTDGIHVQ+ST VTITG T+KTGDDCISIG GT+NLFM +IKCGPGHGVSIGSL
Sbjct: 184 APDQSPNTDGIHVQASTGVTITGVTLKTGDDCISIGPGTRNLFMNNIKCGPGHGVSIGSL 243

Query: 246 GKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNPILIDQ 305
           GK+ NE+GV+N+TL+NAVFT SDNGVRIK+W  PSN FVR+V+FQN++M NV+NPI++DQ
Sbjct: 244 GKDFNEDGVQNITLLNAVFTGSDNGVRIKSWARPSNSFVRNVLFQNLIMNNVQNPIIVDQ 303

Query: 306 NYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLEDIKLTYKN 365
           NYCPN+Q C  QSSGVKI+ VTY++I+GTS T EAVTFDCS ++PC  I+L DIKLTY N
Sbjct: 304 NYCPNNQGCPRQSSGVKISQVTYRNIKGTSKTPEAVTFDCSPTNPCRGIRLHDIKLTYMN 363

Query: 366 KTTTSSCKNIGGSSIGLVVPETC 389
           +  TSSCKNIGG++ G+V+P++C
Sbjct: 364 RAATSSCKNIGGTTFGVVMPKSC 386

BLAST of CSPI01G33450 vs. TrEMBL
Match: B9N8S3_POPTR (Polygalacturonase family protein OS=Populus trichocarpa GN=POPTR_0017s03950g PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 1.8e-158
Identity = 270/389 (69.41%), Postives = 328/389 (84.32%), Query Frame = 1

Query: 1   MANPTKSLVFIVFFHLFIQISTA-ANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRS 60
           MA    S V +  F  F Q + A A+YNVI FGAKPDGKTDSTQ FLKAW++AC S++ S
Sbjct: 1   MAKFVVSYVVLFLFCTFQQSNAASASYNVIKFGAKPDGKTDSTQPFLKAWSAACGSASPS 60

Query: 61  TINVPKGRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVG 120
           TINVPKGR+LL    FRGPCKNKIT  ++GTLVAP DYRALG+SGYWILFIKV+ VS  G
Sbjct: 61  TINVPKGRYLLKATVFRGPCKNKITVQIDGTLVAPTDYRALGNSGYWILFIKVNRVSVFG 120

Query: 121 GSIDGKGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVV 180
           G++D KG  +WAC+ S +NCP GARSITFNWAN+I++SGLTSINSQ  H+VINSCNNV+V
Sbjct: 121 GTLDAKGAGFWACRKSGQNCPVGARSITFNWANDILISGLTSINSQSMHLVINSCNNVLV 180

Query: 181 KNVKIMAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMRDIKCGPGHG 240
           +NV+++APDQSPNTDGIHVQ+ST VTITGST++TGDDCISIG  T+N+ M  IKCGPGHG
Sbjct: 181 RNVRVIAPDQSPNTDGIHVQTSTGVTITGSTLQTGDDCISIGPSTRNMLMSSIKCGPGHG 240

Query: 241 VSIGSLGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKN 300
           +SIGSLGK+ NE GVEN+TL N++F+ SDNGVRIK+W  PSNGFVR+V+FQN++M NV+N
Sbjct: 241 ISIGSLGKDFNEGGVENITLTNSIFSGSDNGVRIKSWARPSNGFVRNVVFQNLIMKNVRN 300

Query: 301 PILIDQNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLEDI 360
           PI++DQNYCPN+Q C  QSSGVKI+ VTY++I+GTSA+ EAVTFDCSSS+PC  IKL+DI
Sbjct: 301 PIIVDQNYCPNNQGCPRQSSGVKISQVTYRNIQGTSASPEAVTFDCSSSNPCRGIKLQDI 360

Query: 361 KLTYKNKTTTSSCKNIGGSSIGLVVPETC 389
           KLTY N   TSSCKNIGG+S G+++PE+C
Sbjct: 361 KLTYMNTAATSSCKNIGGTSSGVLMPESC 389

BLAST of CSPI01G33450 vs. TrEMBL
Match: A0A059AVN6_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H00250 PE=3 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 7.0e-155
Identity = 259/383 (67.62%), Postives = 323/383 (84.33%), Query Frame = 1

Query: 6   KSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRSTINVPK 65
           K ++F++   L +   T A+YNV+ +GAKPDG TD+T++FL+AW +AC S+  +TI +PK
Sbjct: 3   KQVIFLMLTCLVLISPTEADYNVVRYGAKPDGATDATEAFLRAWKAACGSAQAATIYLPK 62

Query: 66  GRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGGSIDGK 125
           GR++L  + FRGPCK+++T  ++GTLVAP DY ALG+SGYWILF +V+ ++ VGG +D K
Sbjct: 63  GRYVLKAVEFRGPCKSRMTVKIDGTLVAPTDYWALGNSGYWILFRQVNKLTVVGGKLDAK 122

Query: 126 GTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVVKNVKIM 185
           G  YWACKNS ++CPPGARSITFNWAN+I+LSGLTSINSQ+TH+VINSCNN VV+NVK++
Sbjct: 123 GAGYWACKNSGRSCPPGARSITFNWANDIVLSGLTSINSQETHIVINSCNNAVVRNVKVI 182

Query: 186 APDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMRDIKCGPGHGVSIGSL 245
           APDQSPNTDGIHVQSST VTITGST++TGDDCISIG GT NL+M  IKCGPGHG+SIGSL
Sbjct: 183 APDQSPNTDGIHVQSSTGVTITGSTLQTGDDCISIGPGTTNLYMSHIKCGPGHGISIGSL 242

Query: 246 GKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNPILIDQ 305
            ++A E+GV+NVTL NAVFT SDNGVRIK+W  PS  FVR+V+FQNIVM NVKNPI+IDQ
Sbjct: 243 ARDATEDGVQNVTLTNAVFTGSDNGVRIKSWARPSTSFVRNVLFQNIVMTNVKNPIIIDQ 302

Query: 306 NYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLEDIKLTYKN 365
           NYCPN+Q C LQSSG KI+ VTY++I GTSAT  AVTFDCS S+PC +I+L+DI LTY N
Sbjct: 303 NYCPNNQGCPLQSSGAKISQVTYRNIRGTSATQNAVTFDCSGSTPCREIRLQDIHLTYMN 362

Query: 366 KTTTSSCKNIGGSSIGLVVPETC 389
           +  TSSCKNIGG+S GL+VP++C
Sbjct: 363 RAATSSCKNIGGTSSGLLVPQSC 385

BLAST of CSPI01G33450 vs. TrEMBL
Match: B9SSX9_RICCO (Polygalacturonase, putative OS=Ricinus communis GN=RCOM_1268300 PE=3 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 9.1e-155
Identity = 262/388 (67.53%), Postives = 327/388 (84.28%), Query Frame = 1

Query: 1   MANPTKSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRST 60
           MAN    ++ + FF    Q+S AA+YNV+ FGAKPDGKTDSTQ FLKAW +ACSS+T ST
Sbjct: 1   MANKIAIMLALSFF--LFQLSNAASYNVLKFGAKPDGKTDSTQPFLKAWAAACSSATAST 60

Query: 61  INVPKGRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGG 120
           I+VPKGR+L+  I FRGPCK++IT  ++GT+ AP+DYRALG+SGYWILFI+V+ +S +GG
Sbjct: 61  ISVPKGRYLIKAIEFRGPCKSRITVKIDGTIEAPVDYRALGNSGYWILFIQVNQISVLGG 120

Query: 121 SIDGKGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVVK 180
           ++D KG  +WAC+ S K+CP GARSITFNWANN+I+SGLTSINSQ  H+VINSCNNV V+
Sbjct: 121 TLDAKGAGFWACRASGKSCPVGARSITFNWANNVIISGLTSINSQTMHLVINSCNNVQVR 180

Query: 181 NVKIMAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMRDIKCGPGHGV 240
           NVK++APDQSPNTDGIHVQ+ST VTITGST++TGDDCISIG GT+NL M  IKCGPGHG+
Sbjct: 181 NVKLIAPDQSPNTDGIHVQTSTGVTITGSTLQTGDDCISIGPGTRNLLMSHIKCGPGHGI 240

Query: 241 SIGSLGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNP 300
           SIGSLG++ NE+GV+N+TL +AVFT SDNGVRIKTW  PS  FVR+V+FQN++M NVKNP
Sbjct: 241 SIGSLGRQFNEDGVQNITLTDAVFTGSDNGVRIKTWARPSTSFVRNVLFQNLIMRNVKNP 300

Query: 301 ILIDQNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLEDIK 360
           I+IDQ+YCP++  C  Q+SGVKI+ V YK+I+GTS + +AVTF+CS S+PC +I+L DIK
Sbjct: 301 IIIDQDYCPDNIGCPNQNSGVKISQVMYKNIQGTSRSPQAVTFECSPSNPCKEIRLHDIK 360

Query: 361 LTYKNKTTTSSCKNIGGSSIGLVVPETC 389
           LTY NK  TSSCKNIGG+S GL +PE+C
Sbjct: 361 LTYMNKAATSSCKNIGGTSSGLAIPESC 386

BLAST of CSPI01G33450 vs. TAIR10
Match: AT2G43890.1 (AT2G43890.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 524.6 bits (1350), Expect = 5.1e-149
Identity = 239/391 (61.13%), Postives = 316/391 (80.82%), Query Frame = 1

Query: 1   MANPTKSLVFIVFFHLFIQIST---AANYNVISFGAKPDGKTDSTQSFLKAWTSACSSST 60
           M N     V ++FF  F+ + +   A+NYNV+SFGAKPDG+TDST++FL AW +AC S+ 
Sbjct: 1   MDNNKLVAVLLMFFSSFLLMKSSTAASNYNVVSFGAKPDGRTDSTKAFLGAWQAACRSAA 60

Query: 61  RSTINVPKGRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSF 120
             T+ VP+G FLL P+ FRGPC+++ITF + GT+VAP DYR LG+SGYWILF+KV+ +S 
Sbjct: 61  AVTVTVPRGSFLLKPVEFRGPCRSRITFQIYGTIVAPSDYRGLGNSGYWILFVKVNRISI 120

Query: 121 VGGSIDGKGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNV 180
           +GG++D +G ++WAC+ S K+CP GARS+TFNWAN++++SGLTSINSQ TH+VINSCNNV
Sbjct: 121 IGGTLDARGASFWACRKSGKSCPVGARSMTFNWANDVVVSGLTSINSQTTHLVINSCNNV 180

Query: 181 VVKNVKIMAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMRDIKCGPG 240
           +V+ VK++APDQSPNTDG+HVQ S  VT+T  T  TGDDCISIG GT+NL+M  + CGPG
Sbjct: 181 IVRKVKLVAPDQSPNTDGLHVQGSAGVTVTDGTFHTGDDCISIGPGTRNLYMSKLNCGPG 240

Query: 241 HGVSIGSLGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINV 300
           HG+SIGSLG++ANE GVEN+TLIN+VF+ SDNGVRIKTW   S GFVR+V+FQN++M NV
Sbjct: 241 HGISIGSLGRDANEAGVENITLINSVFSGSDNGVRIKTWARQSTGFVRNVLFQNLIMKNV 300

Query: 301 KNPILIDQNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLE 360
           +NPI++DQNYCP++Q C  Q SGVKI+ V Y++I+GTS T +A+TFDCS S+PC  I+L 
Sbjct: 301 QNPIIVDQNYCPSNQGCPKQGSGVKISQVVYRNIQGTSRTQQALTFDCSRSNPCQAIRLH 360

Query: 361 DIKLTYKNKTTTSSCKNIGGSSIGLVVPETC 389
           DIKLT+  ++ TS+CKNI G   G+V+P+ C
Sbjct: 361 DIKLTFNGRSATSTCKNIKGVKAGVVMPQGC 391

BLAST of CSPI01G33450 vs. TAIR10
Match: AT1G05660.1 (AT1G05660.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 473.8 bits (1218), Expect = 1.0e-133
Identity = 228/383 (59.53%), Postives = 292/383 (76.24%), Query Frame = 1

Query: 7   SLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRSTINVPKG 66
           SL+F +   + + IS +  +NV+SFGAKPDG TDST +FLKAW  AC S++ +T+ VPKG
Sbjct: 9   SLLFTLLTFIDVSISASNVFNVVSFGAKPDGVTDSTGAFLKAWQGACVSASSATVVVPKG 68

Query: 67  RFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGGSIDGKG 126
            FLL  ITF GPCK+KITF + GT++AP DYR  G+SG+WILF KV+  S VGG+ D + 
Sbjct: 69  TFLLKVITFGGPCKSKITFQVAGTVIAPEDYRTFGNSGFWILFNKVNRFSLVGGTFDARA 128

Query: 127 TAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVVKNVKIMA 186
             +W+C+ S +NCPPG RSI+FN A ++I+SG+ S+NSQ TH+ +N C NVVV+NVK++A
Sbjct: 129 NGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVTHMTLNGCTNVVVRNVKLVA 188

Query: 187 PDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMRDIKCGPGHGVSIGSLG 246
           P  SPNTDG HVQ ST VT TGST++TGDDC++IG GT+NL +  + CGPGHGVSIGSL 
Sbjct: 189 PGNSPNTDGFHVQHSTGVTFTGSTVQTGDDCVAIGPGTRNLLITKLACGPGHGVSIGSLA 248

Query: 247 KEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNPILIDQN 306
           KE  E+GVENVT+ ++VFT S NGVRIK+W  PSNGFVR V FQ++VM NV+NPI+IDQN
Sbjct: 249 KELKEDGVENVTVSSSVFTGSQNGVRIKSWARPSNGFVRTVFFQDLVMKNVENPIIIDQN 308

Query: 307 YCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLEDIKLTY-KN 366
           YCP H+ C  + SGVKI+ VTYK+I+GTSAT EA+   CS SSPC+ I L+DIKLTY K 
Sbjct: 309 YCPTHEGCPNEYSGVKISQVTYKNIQGTSATQEAMKLVCSKSSPCTGITLQDIKLTYNKG 368

Query: 367 KTTTSSCKNIGGSSIGLVVPETC 389
              TS C N  G S+G++ P +C
Sbjct: 369 TPATSFCFNAVGKSLGVIQPTSC 391

BLAST of CSPI01G33450 vs. TAIR10
Match: AT1G05650.1 (AT1G05650.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 462.6 bits (1189), Expect = 2.4e-130
Identity = 225/390 (57.69%), Postives = 296/390 (75.90%), Query Frame = 1

Query: 5   TKSLV-FIVFFHL--FIQISTAAN--YNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRS 64
           TKS + F + F L  FI +S++A+  +NV+SFGAKPDG TDST +FLKAW  AC S+  +
Sbjct: 2   TKSAITFPLIFTLLTFIDVSSSASIVFNVVSFGAKPDGVTDSTAAFLKAWQGACGSAASA 61

Query: 65  TINVPKGRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVG 124
           T+ VP G FLL  ITF GPCK+KITF + GT+VAP DYR  G+SG WILF KV+  S VG
Sbjct: 62  TVVVPTGTFLLKVITFGGPCKSKITFQVTGTVVAPEDYRTFGNSGSWILFNKVNRFSLVG 121

Query: 125 GSIDGKGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVV 184
           G+ D +G+ +W+C+ S +NCPPG RSI+FN A ++I+SG+ S+NSQ +H+ +N C NV V
Sbjct: 122 GTFDARGSGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVSHMTLNGCTNVAV 181

Query: 185 KNVKIMAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMRDIKCGPGHG 244
           +N++++AP  SPNTDG  VQ ST VT+TGST++TGDDC++IG GT+N  +  + CGPGHG
Sbjct: 182 RNIRLVAPGDSPNTDGFTVQFSTGVTLTGSTVQTGDDCVAIGQGTRNFLISKLACGPGHG 241

Query: 245 VSIGSLGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKN 304
           VSIGSL K+ NE+GVENVT+ ++VFT S NGVRIK+W  PS GFVR+V FQN++M NV+N
Sbjct: 242 VSIGSLAKQLNEDGVENVTVSSSVFTGSQNGVRIKSWARPSTGFVRNVFFQNLIMRNVQN 301

Query: 305 PILIDQNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLEDI 364
           PI+IDQNYCP++Q C  + SGVKI  VTYK+I+GTSAT EA+   CS S+PC+ I L+DI
Sbjct: 302 PIIIDQNYCPSNQGCPTEHSGVKITQVTYKNIQGTSATQEAMKLVCSKSNPCTGITLQDI 361

Query: 365 KLTY-KNKTTTSSCKNIGGSSIGLVVPETC 389
           KLTY K    TS C N  G ++G++ P +C
Sbjct: 362 KLTYNKGTPATSLCFNAVGKNLGVIQPTSC 391

BLAST of CSPI01G33450 vs. TAIR10
Match: AT2G43880.1 (AT2G43880.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 439.9 bits (1130), Expect = 1.7e-123
Identity = 207/393 (52.67%), Postives = 290/393 (73.79%), Query Frame = 1

Query: 1   MANPTKSLVFIVFF--HLFIQISTA---ANYNVISFGAKPDGKTDSTQSFLKAWTSACSS 60
           MAN       I+FF  ++F  I ++    ++NV  +GA+ DG+ D+T+SFL AW+ AC S
Sbjct: 1   MANNISFPCAIIFFSINIFFLIKSSHAMPSFNVQRYGARGDGRADATKSFLTAWSLACGS 60

Query: 61  STRSTINVPKGRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGV 120
             R+ + VP+G +L+  + F GPCKN ITF  +GTLVAP +Y  +G+SGYWILF KV+ +
Sbjct: 61  RARAMVYVPRGTYLVKNLVFWGPCKNIITFKNDGTLVAPANYWDIGNSGYWILFAKVNRI 120

Query: 121 SFVGGSIDGKGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCN 180
           S  GG+ID +G  YW+C+    +CP GARSI+F+W NN++LSGL+S NSQ  HV ++  +
Sbjct: 121 SVYGGTIDARGAGYWSCRKKGSHCPQGARSISFSWCNNVLLSGLSSFNSQNMHVTVHHSS 180

Query: 181 NVVVKNVKIMAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMRDIKCG 240
           NV ++NV+I AP  SPNTDGIHVQSS+ VTI+G TI TGDDCI++  G++N+++  + CG
Sbjct: 181 NVRIENVRIRAPSGSPNTDGIHVQSSSGVTISGGTIATGDDCIALSQGSRNIWIERVNCG 240

Query: 241 PGHGVSIGSLGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMI 300
           PGHG+SIGSLG  ANE GV+NVT+ ++VFTK+ NGVRIKTW  PS GFV +V+F+N++M 
Sbjct: 241 PGHGISIGSLGDYANEEGVQNVTVTSSVFTKTQNGVRIKTWARPSRGFVNNVVFRNLIMN 300

Query: 301 NVKNPILIDQNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSSPCSDIK 360
           NV+NP++IDQNYCPN + C  QSSGVKI+ VT+ +I+GTS T  A+  DCS S+ C+ ++
Sbjct: 301 NVENPVIIDQNYCPNGKGCPRQSSGVKISGVTFANIKGTSTTPIAMKLDCSGSNHCTGLR 360

Query: 361 LEDIKLTYKNKTTTSSCKNIGGSSIGLVVPETC 389
           L+DIKLTY  +++ S C+N  G + G++VP  C
Sbjct: 361 LQDIKLTYMRRSSASYCRNAHGRASGVMVPRNC 393

BLAST of CSPI01G33450 vs. TAIR10
Match: AT3G59850.1 (AT3G59850.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 437.2 bits (1123), Expect = 1.1e-122
Identity = 211/384 (54.95%), Postives = 275/384 (71.61%), Query Frame = 1

Query: 7   SLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRSTINVPKG 66
           SL+ ++ F L +  S+A  YN++S+GAKPDGKTDST++F   W  AC+S    TI VPKG
Sbjct: 4   SLLIVLLFLLSVSSSSAQTYNILSYGAKPDGKTDSTKAFTVLWAKACASVKPVTILVPKG 63

Query: 67  RFLLTPITFRGP-CKNK-ITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGGSIDG 126
           RFLL  I F G  CK K +TF + GTLVAP DYR +G   YWILF  +DG+S  GG +D 
Sbjct: 64  RFLLRSIIFDGSKCKRKSVTFRIQGTLVAPSDYRVIGKENYWILFQHLDGISVYGGVLDA 123

Query: 127 KGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVVKNVKI 186
           +G + W+CK S KNCP GA SI F  + N+++SGLTS+NSQ  HV IN C+NV +  VK+
Sbjct: 124 QGASLWSCKKSGKNCPSGATSIGFQSSRNVVISGLTSLNSQMFHVAINGCSNVKLDGVKV 183

Query: 187 MAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMRDIKCGPGHGVSIGS 246
            A   SPNTDGIHVQSS+ V+I  S I TGDDC+SIG GT  L++ ++ CGPGHG+SIGS
Sbjct: 184 SADGNSPNTDGIHVQSSSTVSILNSKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGS 243

Query: 247 LGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNPILID 306
           LGKE+ E GV+N+T+  A FT ++NGVRIK+W  PSNGF +++ FQ+ VM NV+NPI+ID
Sbjct: 244 LGKESVEVGVQNITVKTATFTGTENGVRIKSWARPSNGFAKNIRFQHCVMNNVQNPIVID 303

Query: 307 QNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLEDIKLTYK 366
           QNYCP +++C  Q SG+KI+DV +  I GTSAT   V  DCSS  PC+ I+++D+KLTY+
Sbjct: 304 QNYCPGNENCPNQVSGIKISDVMFFDIHGTSATEVGVKLDCSSKKPCTGIRIQDVKLTYR 363

Query: 367 NKTTTSSCKNIGGSSIGLVVPETC 389
           NK  T+ C + GGS  G   P +C
Sbjct: 364 NKPATTDCSHAGGSEAGFQRPNSC 387

BLAST of CSPI01G33450 vs. NCBI nr
Match: gi|778665426|ref|XP_004142576.2| (PREDICTED: polygalacturonase-like [Cucumis sativus])

HSP 1 Score: 778.1 bits (2008), Expect = 7.3e-222
Identity = 386/389 (99.23%), Postives = 388/389 (99.74%), Query Frame = 1

Query: 1   MANPTKSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRST 60
           MANPTKSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRST
Sbjct: 1   MANPTKSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRST 60

Query: 61  INVPKGRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGG 120
           INVPKGRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGG
Sbjct: 61  INVPKGRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGG 120

Query: 121 SIDGKGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVVK 180
           +IDGKGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVVK
Sbjct: 121 TIDGKGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVVK 180

Query: 181 NVKIMAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMRDIKCGPGHGV 240
           NVKIMAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFM DIKCGPGHGV
Sbjct: 181 NVKIMAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMSDIKCGPGHGV 240

Query: 241 SIGSLGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNP 300
           SIGSLGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNP
Sbjct: 241 SIGSLGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNP 300

Query: 301 ILIDQNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLEDIK 360
           ILIDQNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSS+PCSDIKLEDIK
Sbjct: 301 ILIDQNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSNPCSDIKLEDIK 360

Query: 361 LTYKNKTTTSSCKNIGGSSIGLVVPETCF 390
           LTYKNKTTTSSCKNIGGSSIGLVVPETCF
Sbjct: 361 LTYKNKTTTSSCKNIGGSSIGLVVPETCF 389

BLAST of CSPI01G33450 vs. NCBI nr
Match: gi|659121741|ref|XP_008460791.1| (PREDICTED: polygalacturonase-like [Cucumis melo])

HSP 1 Score: 687.2 bits (1772), Expect = 1.7e-194
Identity = 334/390 (85.64%), Postives = 364/390 (93.33%), Query Frame = 1

Query: 1   MANPTKSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRST 60
           MAN  KSLVFIVFFHLFIQIS+A +YNVI+FGAKPDGKTDSTQSFLKAW SACSS TRST
Sbjct: 1   MANSRKSLVFIVFFHLFIQISSAGSYNVITFGAKPDGKTDSTQSFLKAWKSACSSLTRST 60

Query: 61  INVPKGRFLLTPITFRGPCKNK-ITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVG 120
           INVPKGRFLLT ITFRGPCKN  I FLLNGTLVAPLDY ALGDSGYWILF KV+GVSF+G
Sbjct: 61  INVPKGRFLLTAITFRGPCKNNNIIFLLNGTLVAPLDYNALGDSGYWILFTKVNGVSFIG 120

Query: 121 GSIDGKGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVV 180
           G+IDGKGTAYWACK S K CPPGARSITFNWA+NII+SGLTSINS+QTH+VINSCNNVVV
Sbjct: 121 GNIDGKGTAYWACKGSGKKCPPGARSITFNWASNIIISGLTSINSRQTHIVINSCNNVVV 180

Query: 181 KNVKIMAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMRDIKCGPGHG 240
           KNVKIMAPDQSPNTDGIHVQSST VTI+G+TIKTGDDCISIGDGTKNLFM DIKCGPGHG
Sbjct: 181 KNVKIMAPDQSPNTDGIHVQSSTGVTISGTTIKTGDDCISIGDGTKNLFMSDIKCGPGHG 240

Query: 241 VSIGSLGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKN 300
           VSIGSLGKE NENGVENVTL+NAVFTKSDNGVRIKTWPTPSNGFV+HV+F+NI+M +VK+
Sbjct: 241 VSIGSLGKEVNENGVENVTLVNAVFTKSDNGVRIKTWPTPSNGFVKHVVFENIIMNDVKH 300

Query: 301 PILIDQNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLEDI 360
           PILIDQNYCPN+Q CS+Q SGVKI+DVTYK+IEGTSATSE +TFDCSS SPCS+I+L+DI
Sbjct: 301 PILIDQNYCPNNQRCSVQGSGVKISDVTYKNIEGTSATSEGITFDCSSKSPCSNIRLQDI 360

Query: 361 KLTYKNKTTTSSCKNIGGSSIGLVVPETCF 390
           KLTYKNK  TSSCKNI GS+IGLV P++CF
Sbjct: 361 KLTYKNKAATSSCKNIDGSTIGLVFPQSCF 390

BLAST of CSPI01G33450 vs. NCBI nr
Match: gi|641832567|gb|KDO51599.1| (hypothetical protein CISIN_1g037377mg [Citrus sinensis])

HSP 1 Score: 572.4 bits (1474), Expect = 6.1e-160
Identity = 265/383 (69.19%), Postives = 330/383 (86.16%), Query Frame = 1

Query: 6   KSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRSTINVPK 65
           K ++F++F  +FI+ S AANYNVI++GAKPDG+TDST+ FLKAW SAC S+  STI VPK
Sbjct: 4   KLIIFLLFVIIFIKSSNAANYNVITYGAKPDGRTDSTKPFLKAWASACRSAKASTIYVPK 63

Query: 66  GRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGGSIDGK 125
           GR+L+    FRGPC+N++T  ++GT+VAP +Y ALG+SGYWILFIK+D +S +GG+ DGK
Sbjct: 64  GRYLIKAAEFRGPCRNRVTLQIDGTIVAPTNYWALGNSGYWILFIKIDRLSVIGGTFDGK 123

Query: 126 GTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVVKNVKIM 185
           G  +WAC+ S KNCP GA SITFNWANNI++SGLTSINSQQTH+VINSCNNV V+NVK++
Sbjct: 124 GAGFWACRKSGKNCPAGASSITFNWANNILISGLTSINSQQTHLVINSCNNVNVRNVKVV 183

Query: 186 APDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMRDIKCGPGHGVSIGSL 245
           APDQSPNTDGIHVQ+ST VTITG T+KTGDDCISIG GT+NLFM +IKCGPGHGVSIGSL
Sbjct: 184 APDQSPNTDGIHVQASTGVTITGVTLKTGDDCISIGPGTRNLFMNNIKCGPGHGVSIGSL 243

Query: 246 GKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNPILIDQ 305
           GK+ NE+GV+N+TL+NAVFT SDNGVRIK+W  PSN FVR+V+FQN++M NV+NPI++DQ
Sbjct: 244 GKDFNEDGVQNITLLNAVFTGSDNGVRIKSWARPSNSFVRNVLFQNLIMNNVQNPIIVDQ 303

Query: 306 NYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLEDIKLTYKN 365
           NYCPN+Q C  QSSGVKI+ VTY++I+GTS T EAVTFDCS ++PC  I+L DIKLTY N
Sbjct: 304 NYCPNNQGCPRQSSGVKISQVTYRNIKGTSKTPEAVTFDCSPTNPCRGIRLHDIKLTYMN 363

Query: 366 KTTTSSCKNIGGSSIGLVVPETC 389
           +  TSSCKNIGG++ G+V+P++C
Sbjct: 364 RAATSSCKNIGGTTFGVVMPKSC 386

BLAST of CSPI01G33450 vs. NCBI nr
Match: gi|568880478|ref|XP_006493145.1| (PREDICTED: polygalacturonase-like [Citrus sinensis])

HSP 1 Score: 571.6 bits (1472), Expect = 1.0e-159
Identity = 266/383 (69.45%), Postives = 329/383 (85.90%), Query Frame = 1

Query: 6   KSLVFIVFFHLFIQISTAANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRSTINVPK 65
           K ++F++F  +FI+ S AANYNVI++GAKPDG+TDST+ FLKAW SAC S+  STI VPK
Sbjct: 4   KLIIFLLFVIIFIKSSNAANYNVITYGAKPDGRTDSTKPFLKAWASACRSAKASTIYVPK 63

Query: 66  GRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVGGSIDGK 125
           GR+L+    FRGPC+N++T  ++GT+VAP +Y ALG+SGYWILFIK+D +S +GG+ DGK
Sbjct: 64  GRYLIKAAEFRGPCRNRVTLQIDGTIVAPTNYWALGNSGYWILFIKIDRLSVIGGTFDGK 123

Query: 126 GTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVVKNVKIM 185
           G  +WAC+ S KNCP GA SITFNWANNI++SGLTSINSQQTH+VINSCNNV V+NVK++
Sbjct: 124 GAGFWACRKSGKNCPAGASSITFNWANNILISGLTSINSQQTHLVINSCNNVNVRNVKVV 183

Query: 186 APDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMRDIKCGPGHGVSIGSL 245
           APDQSPNTDGIHVQ+ST VTITG T+KTGDDCISIG GT+NLFM +IKCGPGHGVSIGSL
Sbjct: 184 APDQSPNTDGIHVQASTGVTITGVTLKTGDDCISIGPGTRNLFMNNIKCGPGHGVSIGSL 243

Query: 246 GKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKNPILIDQ 305
           GK  NE+GV+N+TL+NAVFT SDNGVRIK+W  PSN FVR+V+FQNI+M NV+NPI++DQ
Sbjct: 244 GKGFNEDGVQNITLLNAVFTGSDNGVRIKSWARPSNSFVRNVLFQNIIMNNVQNPIIVDQ 303

Query: 306 NYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLEDIKLTYKN 365
           NYCPN+Q C  QSSGVKI+ VTY++I+GTS T EAVTFDCS ++PC  I+L DIKLTY N
Sbjct: 304 NYCPNNQGCPRQSSGVKISQVTYRNIKGTSKTPEAVTFDCSPTNPCRGIRLHDIKLTYMN 363

Query: 366 KTTTSSCKNIGGSSIGLVVPETC 389
           +  TSSCKNIGG++ G+V+P++C
Sbjct: 364 RAATSSCKNIGGTTFGVVMPKSC 386

BLAST of CSPI01G33450 vs. NCBI nr
Match: gi|566211241|ref|XP_006372694.1| (polygalacturonase family protein [Populus trichocarpa])

HSP 1 Score: 567.0 bits (1460), Expect = 2.5e-158
Identity = 270/389 (69.41%), Postives = 328/389 (84.32%), Query Frame = 1

Query: 1   MANPTKSLVFIVFFHLFIQISTA-ANYNVISFGAKPDGKTDSTQSFLKAWTSACSSSTRS 60
           MA    S V +  F  F Q + A A+YNVI FGAKPDGKTDSTQ FLKAW++AC S++ S
Sbjct: 1   MAKFVVSYVVLFLFCTFQQSNAASASYNVIKFGAKPDGKTDSTQPFLKAWSAACGSASPS 60

Query: 61  TINVPKGRFLLTPITFRGPCKNKITFLLNGTLVAPLDYRALGDSGYWILFIKVDGVSFVG 120
           TINVPKGR+LL    FRGPCKNKIT  ++GTLVAP DYRALG+SGYWILFIKV+ VS  G
Sbjct: 61  TINVPKGRYLLKATVFRGPCKNKITVQIDGTLVAPTDYRALGNSGYWILFIKVNRVSVFG 120

Query: 121 GSIDGKGTAYWACKNSAKNCPPGARSITFNWANNIILSGLTSINSQQTHVVINSCNNVVV 180
           G++D KG  +WAC+ S +NCP GARSITFNWAN+I++SGLTSINSQ  H+VINSCNNV+V
Sbjct: 121 GTLDAKGAGFWACRKSGQNCPVGARSITFNWANDILISGLTSINSQSMHLVINSCNNVLV 180

Query: 181 KNVKIMAPDQSPNTDGIHVQSSTNVTITGSTIKTGDDCISIGDGTKNLFMRDIKCGPGHG 240
           +NV+++APDQSPNTDGIHVQ+ST VTITGST++TGDDCISIG  T+N+ M  IKCGPGHG
Sbjct: 181 RNVRVIAPDQSPNTDGIHVQTSTGVTITGSTLQTGDDCISIGPSTRNMLMSSIKCGPGHG 240

Query: 241 VSIGSLGKEANENGVENVTLINAVFTKSDNGVRIKTWPTPSNGFVRHVIFQNIVMINVKN 300
           +SIGSLGK+ NE GVEN+TL N++F+ SDNGVRIK+W  PSNGFVR+V+FQN++M NV+N
Sbjct: 241 ISIGSLGKDFNEGGVENITLTNSIFSGSDNGVRIKSWARPSNGFVRNVVFQNLIMKNVRN 300

Query: 301 PILIDQNYCPNHQSCSLQSSGVKINDVTYKSIEGTSATSEAVTFDCSSSSPCSDIKLEDI 360
           PI++DQNYCPN+Q C  QSSGVKI+ VTY++I+GTSA+ EAVTFDCSSS+PC  IKL+DI
Sbjct: 301 PIIVDQNYCPNNQGCPRQSSGVKISQVTYRNIQGTSASPEAVTFDCSSSNPCRGIKLQDI 360

Query: 361 KLTYKNKTTTSSCKNIGGSSIGLVVPETC 389
           KLTY N   TSSCKNIGG+S G+++PE+C
Sbjct: 361 KLTYMNTAATSSCKNIGGTSSGVLMPESC 389