CSPI01G33230 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI01G33230
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Defensin-like (DEFL) family protein
Location	Chr1 : 28110244 .. 28110627 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAAAGTTGGAGTTATTTCTTGCATTCTCATTGCCTTTCTCGTCTTGTATTCAGGTCGTCTGACTTTATTTATTTATCTTTTTAAATGACTTTAGTCTGAAATTATATGGTTGGATGAAAACCTAATTAAGTGCTTTCTTCTAAAGTAGATACTGTTAATTTGTTAACATTAAGTAATTGATGGAACTGCAGGAAGTTTGAAAACAAGAGCTCAGCTGTGTTGCAATGAACATCCTGAACTGGGGAGTTGCATACCTGGAGTAGACGACAAACATGATAATAGTGGTGAAGATGGAAAGTGTTGGAGTCATTGCATTTCTGGCTGTTTGAAAGGAGGATTTTGCAAGCCAAATAACCATATATGCCATTGTTATTGTTGA

mRNA sequence

ATGGCCAAAGTTGGAGTTATTTCTTGCATTCTCATTGCCTTTCTCGTCTTGTATTCAGGAAGTTTGAAAACAAGAGCTCAGCTGTGTTGCAATGAACATCCTGAACTGGGGAGTTGCATACCTGGAGTAGACGACAAACATGATAATAGTGGTGAAGATGGAAAGTGTTGGAGTCATTGCATTTCTGGCTGTTTGAAAGGAGGATTTTGCAAGCCAAATAACCATATATGCCATTGTTATTGTTGA

Coding sequence (CDS)

ATGGCCAAAGTTGGAGTTATTTCTTGCATTCTCATTGCCTTTCTCGTCTTGTATTCAGGAAGTTTGAAAACAAGAGCTCAGCTGTGTTGCAATGAACATCCTGAACTGGGGAGTTGCATACCTGGAGTAGACGACAAACATGATAATAGTGGTGAAGATGGAAAGTGTTGGAGTCATTGCATTTCTGGCTGTTTGAAAGGAGGATTTTGCAAGCCAAATAACCATATATGCCATTGTTATTGTTGA

Homology

[Close]

BLAST of CSPI01G33230 vs. Swiss-Prot
Match: DEF21_ARATH (Defensin-like protein 21 OS=Arabidopsis thaliana GN=At4g14276 PE=2 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 7.3e-15
Identity = 39/95 (41.05%), Postives = 53/95 (55.79%), Query Frame = 1


Query: 1  MAKVGVISCILIAFLVLYSGSLKTRAQ------------LCCNEHPELGSCIPGVDDKHD 60
          M +  V+S +L A +VL  GS++   Q            +CC EHP +G C+P +D   D
Sbjct: 1  MVRTNVVSFVLFAAIVLCIGSIQIDGQKHTAPWIPEDNSICCKEHPSVGRCLPNID---D 60

Query: 61 NSGEDGKCWSHCISGCLKGGFCK--PNNHICHCYC 82
          ++ + GKCW  CI GC  GGFCK   + HICHC C
Sbjct: 61 SAEKGGKCWKFCIEGCETGGFCKLFGHKHICHCNC 92

[Close]

BLAST of CSPI01G33230 vs. Swiss-Prot
Match: DEF20_ARATH (Putative defensin-like protein 20 OS=Arabidopsis thaliana GN=At5g52605 PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 1.6e-14
Identity = 38/91 (41.76%), Postives = 52/91 (57.14%), Query Frame = 1


Query: 1  MAKVGVISCILIAFLVL--------YSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGE 60
          M +  V+S +L A +VL         +  +     +CC EHP +G C+PG+DD   ++ +
Sbjct: 1  MVRTNVVSFVLFAAIVLCILYGQKHIAPWIFEDKSICCKEHPSVGRCLPGIDD---DAEK 60

Query: 61 DGKCWSHCISGCLKGGFCK--PNNHICHCYC 82
          DGKCW  CI GC  GGFCK   + HICHC C
Sbjct: 61 DGKCWKFCIEGCETGGFCKLFEHKHICHCNC 88

[Close]

BLAST of CSPI01G33230 vs. TrEMBL
Match: A0A0A0LWJ9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573030 PE=4 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 3.0e-23
Identity = 53/84 (63.10%), Postives = 62/84 (73.81%), Query Frame = 1


Query: 1  MAKVGVI-SCILIAFLVLYSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGEDGKCWSH 60
          MAK+G I S + +AFLVLY+GS+ T AQLCCN H ELGSC+PGVDD +     DGKCW H
Sbjct: 1  MAKLGAIFSWVFVAFLVLYAGSMNTNAQLCCNNHRELGSCVPGVDDDY-----DGKCWHH 60

Query: 61 CISGCLKGGFCKP--NNHICHCYC 82
          CI GC +GGFCK     H+CHCYC
Sbjct: 61 CIVGCERGGFCKKLWYGHVCHCYC 79

[Close]

BLAST of CSPI01G33230 vs. TrEMBL
Match: A0A0A0LZ58_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573070 PE=4 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 5.6e-22
Identity = 50/85 (58.82%), Postives = 58/85 (68.24%), Query Frame = 1


Query: 1  MAKVGVISCILIAFLVLYSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGEDGKCWSHC 60
          MAKVG+ISC+L+ FLV YSG+L   A +CCN +P LGSC PGVDD       +GKCW  C
Sbjct: 1  MAKVGLISCVLVVFLVFYSGNLNANAAVCCNNNPSLGSCKPGVDDSPQ---ANGKCWRLC 60

Query: 61 ISGCLKGGFCK----PNNHICHCYC 82
           SGC KGGFCK       H+CHCYC
Sbjct: 61 TSGCTKGGFCKRVGRNGRHVCHCYC 82

[Close]

BLAST of CSPI01G33230 vs. TrEMBL
Match: A0A0A0M1N0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573060 PE=4 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 7.3e-22
Identity = 52/84 (61.90%), Postives = 60/84 (71.43%), Query Frame = 1


Query: 1  MAKVGV-ISCILIAFLVLYSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGEDGKCWSH 60
          MAK+G     + IAFLVLYSGS+ T AQLCCN HPELG C  GVDD +D     GKCW H
Sbjct: 1  MAKLGATFGLVFIAFLVLYSGSMNTNAQLCCNNHPELGECEAGVDDAND-----GKCWLH 60

Query: 61 CISGCLKGGFCK--PNNHICHCYC 82
          CI+GC KGGFCK   + ++CHCYC
Sbjct: 61 CIAGCEKGGFCKHLSDKNVCHCYC 79

[Close]

BLAST of CSPI01G33230 vs. TrEMBL
Match: A0A0A0LYL5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573040 PE=4 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 6.8e-20
Identity = 51/84 (60.71%), Postives = 56/84 (66.67%), Query Frame = 1


Query: 1  MAKVGVISCIL-IAFLVLYSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGEDGKCWSH 60
          MAKVGVIS ++ I FL  YSGSL T AQLCC  HPE G+C PGVDD       DG+CW  
Sbjct: 1  MAKVGVISWVVFIVFLAFYSGSLNTNAQLCCINHPEWGACKPGVDD-----ATDGRCWLK 60

Query: 61 CISGCLKGGFCK--PNNHICHCYC 82
          CI GC KGG CK     H+CHCYC
Sbjct: 61 CIGGCEKGGICKRVGKKHVCHCYC 79

[Close]

BLAST of CSPI01G33230 vs. TrEMBL
Match: R0H1Y0_9BRAS (Uncharacterized protein OS=Capsella rubella GN=CARUB_v10007236mg PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 2.1e-16
Identity = 43/95 (45.26%), Postives = 56/95 (58.95%), Query Frame = 1


Query: 1  MAKVGVISCILIAFLVLYSGSLKTRAQ------------LCCNEHPELGSCIPGVDDKHD 60
          M++  +IS +L A +V+  GS K   Q            +CC EHP LG C+PG+DD   
Sbjct: 1  MSRTKIISLVLFASIVMCMGSNKIDGQKNITPWVNEINSICCREHPSLGRCLPGIDD--- 60

Query: 61 NSGEDGKCWSHCISGCLKGGFCK--PNNHICHCYC 82
          N+ +DGKCW  C+ GC +GGFCK     HICHCYC
Sbjct: 61 NADKDGKCWKFCVEGCERGGFCKLFEKKHICHCYC 92

[Close]

BLAST of CSPI01G33230 vs. TAIR10
Match: AT4G14276.1 (AT4G14276.1 Defensin-like (DEFL) family protein)

HSP 1 Score: 80.9 bits (198), Expect = 4.1e-16
Identity = 39/95 (41.05%), Postives = 53/95 (55.79%), Query Frame = 1


Query: 1  MAKVGVISCILIAFLVLYSGSLKTRAQ------------LCCNEHPELGSCIPGVDDKHD 60
          M +  V+S +L A +VL  GS++   Q            +CC EHP +G C+P +D   D
Sbjct: 1  MVRTNVVSFVLFAAIVLCIGSIQIDGQKHTAPWIPEDNSICCKEHPSVGRCLPNID---D 60

Query: 61 NSGEDGKCWSHCISGCLKGGFCK--PNNHICHCYC 82
          ++ + GKCW  CI GC  GGFCK   + HICHC C
Sbjct: 61 SAEKGGKCWKFCIEGCETGGFCKLFGHKHICHCNC 92

[Close]

BLAST of CSPI01G33230 vs. TAIR10
Match: AT5G52605.1 (AT5G52605.1 Defensin-like (DEFL) family protein)

HSP 1 Score: 79.7 bits (195), Expect = 9.1e-16
Identity = 38/91 (41.76%), Postives = 52/91 (57.14%), Query Frame = 1


Query: 1  MAKVGVISCILIAFLVL--------YSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGE 60
          M +  V+S +L A +VL         +  +     +CC EHP +G C+PG+DD   ++ +
Sbjct: 1  MVRTNVVSFVLFAAIVLCILYGQKHIAPWIFEDKSICCKEHPSVGRCLPGIDD---DAEK 60

Query: 61 DGKCWSHCISGCLKGGFCK--PNNHICHCYC 82
          DGKCW  CI GC  GGFCK   + HICHC C
Sbjct: 61 DGKCWKFCIEGCETGGFCKLFEHKHICHCNC 88

[Close]

BLAST of CSPI01G33230 vs. TAIR10
Match: AT5G39645.1 (AT5G39645.1 Defensin-like (DEFL) family protein)

HSP 1 Score: 49.3 bits (116), Expect = 1.3e-06
Identity = 23/58 (39.66%), Postives = 32/58 (55.17%), Query Frame = 1


Query: 1  MAKVGVISCILIAFLVLYSGSLKTRAQ------------LCCNEHPELGSCIPGVDDK 47
          M K  V+S +L A +VL  GS++   Q            +CC EHP LG C+PG+DD+
Sbjct: 1  MVKTNVVSFVLFAAIVLCIGSIQINGQKHIAPWISEEKSICCREHPSLGRCLPGIDDR 58

[Close]

BLAST of CSPI01G33230 vs. NCBI nr
Match: gi|700211037|gb|KGN66133.1| (hypothetical protein Csa_1G573030 [Cucumis sativus])

HSP 1 Score: 115.5 bits (288), Expect = 4.3e-23
Identity = 53/84 (63.10%), Postives = 62/84 (73.81%), Query Frame = 1


Query: 1  MAKVGVI-SCILIAFLVLYSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGEDGKCWSH 60
          MAK+G I S + +AFLVLY+GS+ T AQLCCN H ELGSC+PGVDD +     DGKCW H
Sbjct: 1  MAKLGAIFSWVFVAFLVLYAGSMNTNAQLCCNNHRELGSCVPGVDDDY-----DGKCWHH 60

Query: 61 CISGCLKGGFCKP--NNHICHCYC 82
          CI GC +GGFCK     H+CHCYC
Sbjct: 61 CIVGCERGGFCKKLWYGHVCHCYC 79

[Close]

BLAST of CSPI01G33230 vs. NCBI nr
Match: gi|700211041|gb|KGN66137.1| (hypothetical protein Csa_1G573070 [Cucumis sativus])

HSP 1 Score: 111.3 bits (277), Expect = 8.0e-22
Identity = 50/85 (58.82%), Postives = 58/85 (68.24%), Query Frame = 1


Query: 1  MAKVGVISCILIAFLVLYSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGEDGKCWSHC 60
          MAKVG+ISC+L+ FLV YSG+L   A +CCN +P LGSC PGVDD       +GKCW  C
Sbjct: 1  MAKVGLISCVLVVFLVFYSGNLNANAAVCCNNNPSLGSCKPGVDDSPQ---ANGKCWRLC 60

Query: 61 ISGCLKGGFCK----PNNHICHCYC 82
           SGC KGGFCK       H+CHCYC
Sbjct: 61 TSGCTKGGFCKRVGRNGRHVCHCYC 82

[Close]

BLAST of CSPI01G33230 vs. NCBI nr
Match: gi|700211040|gb|KGN66136.1| (hypothetical protein Csa_1G573060 [Cucumis sativus])

HSP 1 Score: 110.9 bits (276), Expect = 1.0e-21
Identity = 52/84 (61.90%), Postives = 60/84 (71.43%), Query Frame = 1


Query: 1  MAKVGV-ISCILIAFLVLYSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGEDGKCWSH 60
          MAK+G     + IAFLVLYSGS+ T AQLCCN HPELG C  GVDD +D     GKCW H
Sbjct: 1  MAKLGATFGLVFIAFLVLYSGSMNTNAQLCCNNHPELGECEAGVDDAND-----GKCWLH 60

Query: 61 CISGCLKGGFCK--PNNHICHCYC 82
          CI+GC KGGFCK   + ++CHCYC
Sbjct: 61 CIAGCEKGGFCKHLSDKNVCHCYC 79

[Close]

BLAST of CSPI01G33230 vs. NCBI nr
Match: gi|700211038|gb|KGN66134.1| (hypothetical protein Csa_1G573040 [Cucumis sativus])

HSP 1 Score: 104.4 bits (259), Expect = 9.8e-20
Identity = 51/84 (60.71%), Postives = 56/84 (66.67%), Query Frame = 1


Query: 1  MAKVGVISCIL-IAFLVLYSGSLKTRAQLCCNEHPELGSCIPGVDDKHDNSGEDGKCWSH 60
          MAKVGVIS ++ I FL  YSGSL T AQLCC  HPE G+C PGVDD       DG+CW  
Sbjct: 1  MAKVGVISWVVFIVFLAFYSGSLNTNAQLCCINHPEWGACKPGVDD-----ATDGRCWLK 60

Query: 61 CISGCLKGGFCK--PNNHICHCYC 82
          CI GC KGG CK     H+CHCYC
Sbjct: 61 CIGGCEKGGICKRVGKKHVCHCYC 79

[Close]

BLAST of CSPI01G33230 vs. NCBI nr
Match: gi|727555801|ref|XP_010450072.1| (PREDICTED: putative defensin-like protein 20 [Camelina sativa])

HSP 1 Score: 95.5 bits (236), Expect = 4.6e-17
Identity = 45/95 (47.37%), Postives = 54/95 (56.84%), Query Frame = 1


Query: 1  MAKVGVISCILIAFLVLYSGSLKTRAQ------------LCCNEHPELGSCIPGVDDKHD 60
          M    ++S  L A +VL  G  K   Q            +CC EHP LG C+PG+DDK D
Sbjct: 1  MLSTKIVSLFLFAAIVLCIGFSKVEGQKNIAPWVYNIKSICCREHPSLGRCLPGIDDKAD 60

Query: 61 NSGEDGKCWSHCISGCLKGGFCK--PNNHICHCYC 82
          N   DGKCW +C+ GC +GGFCK   N HICHCYC
Sbjct: 61 N---DGKCWKYCVEGCERGGFCKLFGNKHICHCYC 92

The following BLAST results are available for this feature:

BLAST of CSPI01G33230 vs. Swiss-Prot


Match Name	E-value	Identity	Description
DEF21_ARATH	7.3e-15	41.05	Defensin-like protein 21 OS=Arabidopsis thaliana GN=At4g14276 PE=2 SV=1	[more]
DEF20_ARATH	1.6e-14	41.76	Putative defensin-like protein 20 OS=Arabidopsis thaliana GN=At5g52605 PE=3 SV=1	[more]

BLAST of CSPI01G33230 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LWJ9_CUCSA	3.0e-23	63.10	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573030 PE=4 SV=1	[more]
A0A0A0LZ58_CUCSA	5.6e-22	58.82	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573070 PE=4 SV=1	[more]
A0A0A0M1N0_CUCSA	7.3e-22	61.90	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573060 PE=4 SV=1	[more]
A0A0A0LYL5_CUCSA	6.8e-20	60.71	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G573040 PE=4 SV=1	[more]
R0H1Y0_9BRAS	2.1e-16	45.26	Uncharacterized protein OS=Capsella rubella GN=CARUB_v10007236mg PE=4 SV=1	[more]

BLAST of CSPI01G33230 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G14276.1	4.1e-16	41.05	Defensin-like (DEFL) family protein	[more]
AT5G52605.1	9.1e-16	41.76	Defensin-like (DEFL) family protein	[more]
AT5G39645.1	1.3e-06	39.66	Defensin-like (DEFL) family protein	[more]

BLAST of CSPI01G33230 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700211037\|gb\|KGN66133.1\|	4.3e-23	63.10	hypothetical protein Csa_1G573030 [Cucumis sativus]	[more]
gi\|700211041\|gb\|KGN66137.1\|	8.0e-22	58.82	hypothetical protein Csa_1G573070 [Cucumis sativus]	[more]
gi\|700211040\|gb\|KGN66136.1\|	1.0e-21	61.90	hypothetical protein Csa_1G573060 [Cucumis sativus]	[more]
gi\|700211038\|gb\|KGN66134.1\|	9.8e-20	60.71	hypothetical protein Csa_1G573040 [Cucumis sativus]	[more]
gi\|727555801\|ref\|XP_010450072.1\|	4.6e-17	47.37	PREDICTED: putative defensin-like protein 20 [Camelina sativa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR022618	Defensin-like

Vocabulary: **Cellular Component**
Term	Definition
GO:0005576	extracellular region

Vocabulary: **Biological Process**
Term	Definition
GO:0050832	defense response to fungus

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050832	defense response to fungus
biological_process	GO:0008150	biological_process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G33230.1	CSPI01G33230.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR022618	Defensin-like protein	PFAM	PF10868	Defensin_like	coord: 28..81 score: 1.0
None	No IPR available	PANTHER	PTHR34453	FAMILY NOT NAMED	coord: 2..81 score: 1.9
None	No IPR available	PANTHER	PTHR34453:SF1	DEFENSIN-LIKE (DEFL) FAMILY PROTEIN-RELATED	coord: 2..81 score: 1.9

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI01G33230	Cucsa.027340	Cucumber (Gy14) v1	cgycpiB584
CSPI01G33230	CsGy1G031950	Cucumber (Gy14) v2	cgybcpiB002

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI01G33230	Cucumber (Chinese Long) v3	cpicucB000
CSPI01G33230	Cucumber (Chinese Long) v2	cpicuB001
CSPI01G33230	Melon (DHL92) v3.5.1	cpimeB044
CSPI01G33230	Bottle gourd (USVL1VR-Ls)	cpilsiB059
CSPI01G33230	Melon (DHL92) v3.6.1	cpimedB038