CSPI01G32180 (gene) Wild cucumber (PI 183967)

NameCSPI01G32180
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionMatrix metalloproteinase, putative
LocationChr1 : 26951592 .. 26952536 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAATAAACCTTTGGCAATTCTTCTCTTGATCATCTCTATCTTCCTTCCTCTTTGCTTTTCACTTCCATTGATCCAAGTTTCTCCATTTGCATTTCTGAATGACCTCCAAGGATGCAAGAAAGGTGATAATGTCAAAGGCATATCCAAGCTTAAGAACTTTTTCCGTTATTATGGTTATTTAAACCATCAAATCAATGCCACTGGTCATTTGATCGACATTGATGCCAACGACATCTTTGATGATCGCTTGGAGTCTGCCATCAAAACCTACCAACAATACTTCCATCTAAACCCTACTGGATCGCTGAATGCCGAGACGTTATCCCAACTTGCAACACCTCGGTGCGGCAATCCAGATATCATTAATGAAACCACTGGTAGAATGCTATCAGAAGATATCGACAACGTTAGTAGTCATGATCACCATCACCTTCCCCATGCTGTATCACATTATGCTTTCTTCCCAGGAAGGCTTAGGTGGCCATCTACCAAATATCGCCTCACATATGCGTTTCTTCCTGGCACTCGTGCAGATGCAAAAGCACCAGTGGCACGTGCGTTTGCAACATGGGCTCGAAACACACATTTTAAGTTTACTTTGGTCACAAACTATAGAAGAGCTGATTTGAAGATAGGATTTTATAGAGGAAACCATGGAGATGGGTACCCATTTGACGGACCGGGAGGAACTTTGGCACATGCTTTTGCTCCAACCGATGGGAGATTTCATTATGATTCAACAGAGAAATGGGCAGTTGGGGCAGTGAGAGGGAGATATGATTTGCAGACAGTGGCTTTGCATGAGATTGGACATCTTCTTGGACTTGGGCATAGCACAGTTAAAAATGCTATAATGTATCCTTATATAAAATCTGGGTCCACTAAGGGTTTGAATGTTGATGACATTAAAGGGATCAAGGTTTTATACAACAGACGTTAG

mRNA sequence

ATGGGGAATAAACCTTTGGCAATTCTTCTCTTGATCATCTCTATCTTCCTTCCTCTTTGCTTTTCACTTCCATTGATCCAAGTTTCTCCATTTGCATTTCTGAATGACCTCCAAGGATGCAAGAAAGGTGATAATGTCAAAGGCATATCCAAGCTTAAGAACTTTTTCCGTTATTATGGTTATTTAAACCATCAAATCAATGCCACTGGTCATTTGATCGACATTGATGCCAACGACATCTTTGATGATCGCTTGGAGTCTGCCATCAAAACCTACCAACAATACTTCCATCTAAACCCTACTGGATCGCTGAATGCCGAGACGTTATCCCAACTTGCAACACCTCGGTGCGGCAATCCAGATATCATTAATGAAACCACTGGTAGAATGCTATCAGAAGATATCGACAACGTTAGTAGTCATGATCACCATCACCTTCCCCATGCTGTATCACATTATGCTTTCTTCCCAGGAAGGCTTAGGTGGCCATCTACCAAATATCGCCTCACATATGCGTTTCTTCCTGGCACTCGTGCAGATGCAAAAGCACCAGTGGCACGTGCGTTTGCAACATGGGCTCGAAACACACATTTTAAGTTTACTTTGGTCACAAACTATAGAAGAGCTGATTTGAAGATAGGATTTTATAGAGGAAACCATGGAGATGGGTACCCATTTGACGGACCGGGAGGAACTTTGGCACATGCTTTTGCTCCAACCGATGGGAGATTTCATTATGATTCAACAGAGAAATGGGCAGTTGGGGCAGTGAGAGGGAGATATGATTTGCAGACAGTGGCTTTGCATGAGATTGGACATCTTCTTGGACTTGGGCATAGCACAGTTAAAAATGCTATAATGTATCCTTATATAAAATCTGGGTCCACTAAGGGTTTGAATGTTGATGACATTAAAGGGATCAAGGTTTTATACAACAGACGTTAG

Coding sequence (CDS)

ATGGGGAATAAACCTTTGGCAATTCTTCTCTTGATCATCTCTATCTTCCTTCCTCTTTGCTTTTCACTTCCATTGATCCAAGTTTCTCCATTTGCATTTCTGAATGACCTCCAAGGATGCAAGAAAGGTGATAATGTCAAAGGCATATCCAAGCTTAAGAACTTTTTCCGTTATTATGGTTATTTAAACCATCAAATCAATGCCACTGGTCATTTGATCGACATTGATGCCAACGACATCTTTGATGATCGCTTGGAGTCTGCCATCAAAACCTACCAACAATACTTCCATCTAAACCCTACTGGATCGCTGAATGCCGAGACGTTATCCCAACTTGCAACACCTCGGTGCGGCAATCCAGATATCATTAATGAAACCACTGGTAGAATGCTATCAGAAGATATCGACAACGTTAGTAGTCATGATCACCATCACCTTCCCCATGCTGTATCACATTATGCTTTCTTCCCAGGAAGGCTTAGGTGGCCATCTACCAAATATCGCCTCACATATGCGTTTCTTCCTGGCACTCGTGCAGATGCAAAAGCACCAGTGGCACGTGCGTTTGCAACATGGGCTCGAAACACACATTTTAAGTTTACTTTGGTCACAAACTATAGAAGAGCTGATTTGAAGATAGGATTTTATAGAGGAAACCATGGAGATGGGTACCCATTTGACGGACCGGGAGGAACTTTGGCACATGCTTTTGCTCCAACCGATGGGAGATTTCATTATGATTCAACAGAGAAATGGGCAGTTGGGGCAGTGAGAGGGAGATATGATTTGCAGACAGTGGCTTTGCATGAGATTGGACATCTTCTTGGACTTGGGCATAGCACAGTTAAAAATGCTATAATGTATCCTTATATAAAATCTGGGTCCACTAAGGGTTTGAATGTTGATGACATTAAAGGGATCAAGGTTTTATACAACAGACGTTAG
BLAST of CSPI01G32180 vs. Swiss-Prot
Match: 3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 4.7e-62
Identity = 128/291 (43.99%), Postives = 170/291 (58.42%), Query Frame = 1

Query: 32  AFLNDLQGCKKGDNVKGISKLKNFFRYYGYLNHQINATGHLIDIDANDIFDDRLESAIKT 91
           +FLN   GC  G    G+  LK +F+++GY+  + N +G+       D FDD L++A++ 
Sbjct: 45  SFLN-FTGCHAGKKYDGLYMLKQYFQHFGYIT-ETNLSGNF-----TDDFDDILKNAVEM 104

Query: 92  YQQYFHLNPTGSLNAETLSQLATPRCGNPDIINETTGRMLSEDIDNVSSHDHHHLPHAVS 151
           YQ+ F LN TG L+  TL  +  PRCGNPD++N T+          VS        HAV 
Sbjct: 105 YQRNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVK 164

Query: 152 HYAFFPGRLRWPSTKYRLTYAFLP--GTRADAKAPVARAFATWARNTHFKFTLVTNYRRA 211
           HY+FFPG  RWP  +  LTYAF P      + K+  +RAF  W   T   FT V  +  +
Sbjct: 165 HYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTS 224

Query: 212 DLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVG--------AVRGRY 271
           D+ IGFY G HGDG PFDGP  TLAHAF+P  G FH D  E W V         +V    
Sbjct: 225 DISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAV 284

Query: 272 DLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTK-GLNVDDIKGIKVLY 312
           DL++VA+HEIGHLLGLGHS+V+ +IMYP I++G  K  L  DD++G++ LY
Sbjct: 285 DLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

BLAST of CSPI01G32180 vs. Swiss-Prot
Match: 2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.0e-61
Identity = 124/291 (42.61%), Postives = 175/291 (60.14%), Query Frame = 1

Query: 35  NDLQGCKKGDNVKGISKLKNFFRYYGYLNHQINATGHLIDIDANDIFDDRLESAIKTYQQ 94
           ++  GC  G NV G+ ++K +F+ +GY+    +        +  D FDD L++A++ YQ 
Sbjct: 45  SNFTGCHHGQNVDGLYRIKKYFQRFGYIPETFSG-------NFTDDFDDILKAAVELYQT 104

Query: 95  YFHLNPTGSLNAETLSQLATPRCGNPDIINETT----GRMLSEDIDNVSSHDHHHLPHAV 154
            F+LN TG L+A T+  +  PRCGNPD++N T+    GR  + +++   +H      HAV
Sbjct: 105 NFNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTH-----LHAV 164

Query: 155 SHYAFFPGRLRWPSTKYRLTYAFLPGT--RADAKAPVARAFATWARNTHFKFTLVTNYRR 214
             Y  FPG  RWP  +  LTYAF P      + K+  +RAF  W+  T   FTL  ++  
Sbjct: 165 KRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFST 224

Query: 215 ADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVG-------AVRGRY 274
           +D+ IGFY G+HGDG PFDG  GTLAHAF+P  G+FH D+ E W V        +V    
Sbjct: 225 SDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAV 284

Query: 275 DLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTK-GLNVDDIKGIKVLY 312
           DL++VA+HEIGHLLGLGHS+V+ +IMYP I +G  K  L  DD++GI+ LY
Sbjct: 285 DLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

BLAST of CSPI01G32180 vs. Swiss-Prot
Match: 5MMP_ARATH (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 2.9e-59
Identity = 143/331 (43.20%), Postives = 180/331 (54.38%), Query Frame = 1

Query: 9   LLLIISIFL----PLC--FSLPLIQVSPFAFLNDLQ----------GCKKGDNVKGISKL 68
           LLL I IF     P+   F   +  + P  FLN  Q          GC  G+N+ G+SKL
Sbjct: 4   LLLTILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKL 63

Query: 69  KNFFRYYGYLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQL 128
           K +FR +GY+    N T         D FDD L+SAI TYQ+ F+L  TG L++ TL Q+
Sbjct: 64  KQYFRRFGYITTTGNCT---------DDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQI 123

Query: 129 ATPRCGNPDIINETTGRMLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYA 188
             PRCGNPD+I            D VS  +   +      Y+FFPG+ RWP  K  LTYA
Sbjct: 124 VKPRCGNPDLI------------DGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYA 183

Query: 189 FLPGTRA--DAKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPG 248
           F P      + K   +RAF  WA  T   FT   +  RAD+ IGF+ G HGDG PFDG  
Sbjct: 184 FAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAM 243

Query: 249 GTLAHAFAPTDGRFHYDSTEKWAV--GAVRGR-------YDLQTVALHEIGHLLGLGHST 308
           GTLAHA +P  G  H D  E W +  G +  R        DL++VA+HEIGHLLGLGHS+
Sbjct: 244 GTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSS 303

Query: 309 VKNAIMYPYIKSGSTK-GLNVDDIKGIKVLY 312
           V++AIM+P I  G  K  L  DDI+GI+ LY
Sbjct: 304 VEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

BLAST of CSPI01G32180 vs. Swiss-Prot
Match: MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2)

HSP 1 Score: 216.1 bits (549), Expect = 5.6e-55
Identity = 134/320 (41.88%), Postives = 175/320 (54.69%), Query Frame = 1

Query: 9   LLLIISIFLPLCFSLPLIQV-SPFAFLND----LQGCKKGDNVKGISKLKNFFRYYGYLN 68
           LL+ ++    L  SLP +    P+A+  +          G N KG+S +KN+F + GY+ 
Sbjct: 9   LLVALATLYFLATSLPSVSAHGPYAWDGEATYKFTTYHPGQNYKGLSNVKNYFHHLGYIP 68

Query: 69  HQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNPDII 128
           +  +          +D FDD L SAIKTYQ+ ++LN TG  +  TL Q+ TPRCG PDII
Sbjct: 69  NAPHF---------DDNFDDTLVSAIKTYQKNYNLNVTGKFDINTLKQIMTPRCGVPDII 128

Query: 129 ---NETTGRMLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRAD 188
              N+TT   +                  +S Y FF    RW +   +LTYAF P  R D
Sbjct: 129 INTNKTTSFGM------------------ISDYTFFKDMPRWQAGTTQLTYAFSPEPRLD 188

Query: 189 A--KAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFA 248
              K+ +ARAF+ W    +  F   T+Y  A++KI F   NHGD YPFDGPGG L HAFA
Sbjct: 189 DTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFA 248

Query: 249 PTDGRFHYDSTEKWAVGA------VRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIK 308
           PTDGR H+D+ E W          V   +DL++VA+HEIGHLLGLGHS+   AIMYP I 
Sbjct: 249 PTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIP 301

Query: 309 SGSTK-GLNVDDIKGIKVLY 312
             + K  L  DDI GI+ LY
Sbjct: 309 PRTRKVNLAQDDIDGIRKLY 301

BLAST of CSPI01G32180 vs. Swiss-Prot
Match: 1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.3e-47
Identity = 112/281 (39.86%), Postives = 152/281 (54.09%), Query Frame = 1

Query: 43  GDNVKGISKLKNFFRYYGYLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTG 102
           G +V G+S+LK +   +GY+N              +D+FD  LESAI  YQ+   L  TG
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIF--------SDVFDGPLESAISLYQENLGLPITG 123

Query: 103 SLNAETLSQLATPRCGNPDIINETTGRMLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRW 162
            L+  T++ ++ PRCG  D     T   ++ D             H  +HY +F G+ +W
Sbjct: 124 RLDTSTVTLMSLPRCGVSD-----THMTINNDF-----------LHTTAHYTYFNGKPKW 183

Query: 163 PSTKYRLTYAFLPG------TRADAKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFY 222
              +  LTYA          T  D K    RAF+ W+      F  V ++  ADLKIGFY
Sbjct: 184 --NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFY 243

Query: 223 RGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAV-----GAVRGRYDLQTVALHEI 282
            G+HGDG PFDG  GTLAHAFAP +GR H D+ E W V     G+     DL++VA HEI
Sbjct: 244 AGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEI 303

Query: 283 GHLLGLGHSTVKNAIMYPYIKSGSTK-GLNVDDIKGIKVLY 312
           GHLLGLGHS+ ++A+MYP ++  + K  L VDD+ G+  LY
Sbjct: 304 GHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

BLAST of CSPI01G32180 vs. TrEMBL
Match: A0A0A0M0J6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G654860 PE=3 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 3.4e-184
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 1

Query: 1   MGNKPLAILLLIISIFLPLCFSLPLIQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYG 60
           MGNKPLAILLLIISIFLPLCFSLPLIQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYG
Sbjct: 1   MGNKPLAILLLIISIFLPLCFSLPLIQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYG 60

Query: 61  YLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNP 120
           YLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNP
Sbjct: 61  YLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNP 120

Query: 121 DIINETTGRMLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRAD 180
           DIINETTGRMLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRAD
Sbjct: 121 DIINETTGRMLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRAD 180

Query: 181 AKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPT 240
           AKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPT
Sbjct: 181 AKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPT 240

Query: 241 DGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLN 300
           DGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLN
Sbjct: 241 DGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLN 300

Query: 301 VDDIKGIKVLYNRR 315
           VDDIKGIKVLYNRR
Sbjct: 301 VDDIKGIKVLYNRR 314

BLAST of CSPI01G32180 vs. TrEMBL
Match: A0A0A0M032_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G654840 PE=3 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 1.6e-146
Identity = 258/313 (82.43%), Postives = 275/313 (87.86%), Query Frame = 1

Query: 1   MGNKPLAILLLIISIFLPLCFSLPLIQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYG 60
           MG+KPLAILL I    LPLCFSLPL QVS  +FLNDLQG KKGDNVKGISKLKNFFR YG
Sbjct: 1   MGSKPLAILLFIS--ILPLCFSLPLRQVSRLSFLNDLQGSKKGDNVKGISKLKNFFRRYG 60

Query: 61  YLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNP 120
           YLNHQIN TGHLID DA+D FDDR ESA+KTYQQYFHLN TGSLNAETLSQLATPRCGNP
Sbjct: 61  YLNHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPRCGNP 120

Query: 121 DIINETTGRMLSEDIDNVSSHDHHH-LPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRA 180
           DI+NE TGRML E+ +N SSHDH+H L HAV HY+FFPGR RWP TKY LTY FLP T A
Sbjct: 121 DILNEATGRMLLENNNNDSSHDHYHQLSHAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHA 180

Query: 181 DAKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAP 240
           DAKAPV RAFATWAR+THFKF+L TN RRADLKIGFYRGNHGDGYPFDG GGTLAHAF P
Sbjct: 181 DAKAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTP 240

Query: 241 TDGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGL 300
           TDGR H+DSTEKW VGAVRGR+DL+TVALHEIGHLLGLGHS VKNAIMYP I+SGSTKGL
Sbjct: 241 TDGRVHFDSTEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTKGL 300

Query: 301 NVDDIKGIKVLYN 313
           N DDI+GI+VLYN
Sbjct: 301 NADDIEGIEVLYN 311

BLAST of CSPI01G32180 vs. TrEMBL
Match: B9RUG6_RICCO (Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 3.0e-92
Identity = 179/322 (55.59%), Postives = 224/322 (69.57%), Query Frame = 1

Query: 1   MGNKPLAILLLIISIFLPLCFSLPLI-------QVSPFAFLNDLQGCKKGDNVKGISKLK 60
           M +KP AI  + + + + L  S  L        + S F FL  LQGC KGDN+KGI  LK
Sbjct: 1   MASKPFAIFSVTLILLISLLSSAALAHSHSKTEKSSAFDFLKHLQGCHKGDNLKGIHDLK 60

Query: 61  NFFRYYGYLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLA 120
            +   +GYL+++  +  +      +D FDD LE A+KTYQ  +HLN TG L++ET++++ 
Sbjct: 61  KYLENFGYLSYKNQSHSN------DDDFDDLLEYALKTYQFNYHLNVTGFLDSETVTKMM 120

Query: 121 TPRCGNPDIINETTGRMLSEDIDNVSSHDHHHLP---HAVSHYAFFPGRLRWPSTKYRLT 180
            PRCG  DIIN TT RM S +      + HHH     H VSHY FFPG  RWP++KY LT
Sbjct: 121 MPRCGVADIINGTT-RMQSSN-----KNPHHHSSTSFHTVSHYEFFPGNPRWPASKYHLT 180

Query: 181 YAFLPGTRADAKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPG 240
           Y FLPGT   A  PVA+AF TWA NTHF+FT V +YR AD+ IGF+RG+HGDG PFDG G
Sbjct: 181 YGFLPGTPNQAMEPVAKAFQTWAANTHFRFTRVQDYRAADITIGFHRGDHGDGSPFDGRG 240

Query: 241 GTLAHAFAPTDGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPY 300
           GTLAHAFAP DGRFHYD  E WAVGA +G +D++TVALHEIGHLLGLGHS+V+ AIM+P 
Sbjct: 241 GTLAHAFAPQDGRFHYDGDEHWAVGATQGAFDVETVALHEIGHLLGLGHSSVEGAIMHPS 300

Query: 301 IKSGSTKGLNVDDIKGIKVLYN 313
           I+SG+TKGL+ DDI+GI+ LYN
Sbjct: 301 IQSGATKGLHSDDIQGIRALYN 310

BLAST of CSPI01G32180 vs. TrEMBL
Match: S1SMU3_THECC (Matrixin family protein OS=Theobroma cacao GN=TCM_044196 PE=3 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.4e-89
Identity = 180/318 (56.60%), Postives = 213/318 (66.98%), Query Frame = 1

Query: 1   MGNKPLAILLLIISIFLPLCFSLPLIQVS-----PFAFLNDLQGCKKGDNVKGISKLKNF 60
           M    ++ L     + LPL F   L         PF FL  LQGC KGD VK I KLK +
Sbjct: 1   MAYNAISFLSFCTLLVLPLLFQATLADSKDKKPYPFDFLKHLQGCHKGDKVKDIRKLKKY 60

Query: 61  FRYYGYLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATP 120
              +GYL++  N T H  D D    FDD LESAIKTYQ  FHLN  G+L+ ET+S++  P
Sbjct: 61  LEQFGYLSYSKNKT-HANDDD----FDDLLESAIKTYQLNFHLNSNGALDTETVSKMMMP 120

Query: 121 RCGNPDIINETTGRMLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLP 180
           RCG  DIIN T+G    +   + ++       H VSHYAFFP   RWP +K  LTYAFLP
Sbjct: 121 RCGVADIINGTSGMRSGKKKPHRAAGSKS--IHEVSHYAFFPRSPRWPPSKSHLTYAFLP 180

Query: 181 GTRADAKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAH 240
           GTRADA  PVA AF TWA NTHF+F+ + NYR AD+ IGF R +HGDG PFDGPGGTLAH
Sbjct: 181 GTRADAVNPVAGAFQTWAANTHFRFSRIDNYRDADITIGFQRRDHGDGNPFDGPGGTLAH 240

Query: 241 AFAPTDGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGS 300
           AFAPT GRFHYD+ E W+V A  G   L+TVALHEIGHLLGLGHS+++NAIMYP I +G+
Sbjct: 241 AFAPTLGRFHYDADETWSVSARPGTMHLETVALHEIGHLLGLGHSSIENAIMYPSITAGT 300

Query: 301 TKGLNVDDIKGIKVLYNR 314
           +KGL  DDI+GIK LYNR
Sbjct: 301 SKGLARDDIEGIKALYNR 311

BLAST of CSPI01G32180 vs. TrEMBL
Match: A0A061FPL5_THECC (Matrixin family protein OS=Theobroma cacao GN=TCM_044201 PE=3 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 9.2e-89
Identity = 179/318 (56.29%), Postives = 212/318 (66.67%), Query Frame = 1

Query: 1   MGNKPLAILLLIISIFLPLCFSLPLIQVS-----PFAFLNDLQGCKKGDNVKGISKLKNF 60
           M    ++ L     + LPL F   L         PF FL  LQGC KGD VK I KLK +
Sbjct: 1   MAYNAISFLSFCTLLVLPLLFQATLADSKDKKPYPFDFLKHLQGCHKGDKVKDIRKLKKY 60

Query: 61  FRYYGYLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATP 120
              +GYL++  N T H  D D    FDD LESAIKTYQ  FHLN  G+L+ ET+S++  P
Sbjct: 61  LEQFGYLSYSKNKT-HANDND----FDDLLESAIKTYQLNFHLNSNGALDTETVSKMMMP 120

Query: 121 RCGNPDIINETTGRMLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLP 180
           RCG  DIIN T+G    +   + ++       H VSHYAFFP   RWP +K  LTYAFLP
Sbjct: 121 RCGVADIINGTSGMRSGKKKPHRAAGSKS--IHEVSHYAFFPRSPRWPPSKSHLTYAFLP 180

Query: 181 GTRADAKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAH 240
           GTRADA  PVA AF TWA NTHF+F+ + NYR AD+ IGF R +HGDG PFDGPGGTLAH
Sbjct: 181 GTRADAVNPVAGAFQTWAANTHFRFSRIDNYRDADITIGFQRRDHGDGNPFDGPGGTLAH 240

Query: 241 AFAPTDGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGS 300
           AFAPT GRFHYD+ E W+V A  G   L+TVALHEIGHLLGL HS+++NAIMYP I +G+
Sbjct: 241 AFAPTIGRFHYDADETWSVSARPGTMHLETVALHEIGHLLGLSHSSIENAIMYPSITAGT 300

Query: 301 TKGLNVDDIKGIKVLYNR 314
           +KGL  DDI+GIK LYNR
Sbjct: 301 SKGLARDDIEGIKALYNR 311

BLAST of CSPI01G32180 vs. TAIR10
Match: AT1G24140.1 (AT1G24140.1 Matrixin family protein)

HSP 1 Score: 239.6 bits (610), Expect = 2.6e-63
Identity = 128/291 (43.99%), Postives = 170/291 (58.42%), Query Frame = 1

Query: 32  AFLNDLQGCKKGDNVKGISKLKNFFRYYGYLNHQINATGHLIDIDANDIFDDRLESAIKT 91
           +FLN   GC  G    G+  LK +F+++GY+  + N +G+       D FDD L++A++ 
Sbjct: 45  SFLN-FTGCHAGKKYDGLYMLKQYFQHFGYIT-ETNLSGNF-----TDDFDDILKNAVEM 104

Query: 92  YQQYFHLNPTGSLNAETLSQLATPRCGNPDIINETTGRMLSEDIDNVSSHDHHHLPHAVS 151
           YQ+ F LN TG L+  TL  +  PRCGNPD++N T+          VS        HAV 
Sbjct: 105 YQRNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVK 164

Query: 152 HYAFFPGRLRWPSTKYRLTYAFLP--GTRADAKAPVARAFATWARNTHFKFTLVTNYRRA 211
           HY+FFPG  RWP  +  LTYAF P      + K+  +RAF  W   T   FT V  +  +
Sbjct: 165 HYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTS 224

Query: 212 DLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVG--------AVRGRY 271
           D+ IGFY G HGDG PFDGP  TLAHAF+P  G FH D  E W V         +V    
Sbjct: 225 DISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAV 284

Query: 272 DLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTK-GLNVDDIKGIKVLY 312
           DL++VA+HEIGHLLGLGHS+V+ +IMYP I++G  K  L  DD++G++ LY
Sbjct: 285 DLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

BLAST of CSPI01G32180 vs. TAIR10
Match: AT1G70170.1 (AT1G70170.1 matrix metalloproteinase)

HSP 1 Score: 236.9 bits (603), Expect = 1.7e-62
Identity = 124/291 (42.61%), Postives = 175/291 (60.14%), Query Frame = 1

Query: 35  NDLQGCKKGDNVKGISKLKNFFRYYGYLNHQINATGHLIDIDANDIFDDRLESAIKTYQQ 94
           ++  GC  G NV G+ ++K +F+ +GY+    +        +  D FDD L++A++ YQ 
Sbjct: 45  SNFTGCHHGQNVDGLYRIKKYFQRFGYIPETFSG-------NFTDDFDDILKAAVELYQT 104

Query: 95  YFHLNPTGSLNAETLSQLATPRCGNPDIINETT----GRMLSEDIDNVSSHDHHHLPHAV 154
            F+LN TG L+A T+  +  PRCGNPD++N T+    GR  + +++   +H      HAV
Sbjct: 105 NFNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTH-----LHAV 164

Query: 155 SHYAFFPGRLRWPSTKYRLTYAFLPGT--RADAKAPVARAFATWARNTHFKFTLVTNYRR 214
             Y  FPG  RWP  +  LTYAF P      + K+  +RAF  W+  T   FTL  ++  
Sbjct: 165 KRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFST 224

Query: 215 ADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVG-------AVRGRY 274
           +D+ IGFY G+HGDG PFDG  GTLAHAF+P  G+FH D+ E W V        +V    
Sbjct: 225 SDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAV 284

Query: 275 DLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTK-GLNVDDIKGIKVLY 312
           DL++VA+HEIGHLLGLGHS+V+ +IMYP I +G  K  L  DD++GI+ LY
Sbjct: 285 DLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

BLAST of CSPI01G32180 vs. TAIR10
Match: AT1G59970.1 (AT1G59970.1 Matrixin family protein)

HSP 1 Score: 230.3 bits (586), Expect = 1.6e-60
Identity = 143/331 (43.20%), Postives = 180/331 (54.38%), Query Frame = 1

Query: 9   LLLIISIFL----PLC--FSLPLIQVSPFAFLNDLQ----------GCKKGDNVKGISKL 68
           LLL I IF     P+   F   +  + P  FLN  Q          GC  G+N+ G+SKL
Sbjct: 4   LLLTILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKL 63

Query: 69  KNFFRYYGYLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQL 128
           K +FR +GY+    N T         D FDD L+SAI TYQ+ F+L  TG L++ TL Q+
Sbjct: 64  KQYFRRFGYITTTGNCT---------DDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQI 123

Query: 129 ATPRCGNPDIINETTGRMLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYA 188
             PRCGNPD+I            D VS  +   +      Y+FFPG+ RWP  K  LTYA
Sbjct: 124 VKPRCGNPDLI------------DGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYA 183

Query: 189 FLPGTRA--DAKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPG 248
           F P      + K   +RAF  WA  T   FT   +  RAD+ IGF+ G HGDG PFDG  
Sbjct: 184 FAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAM 243

Query: 249 GTLAHAFAPTDGRFHYDSTEKWAV--GAVRGR-------YDLQTVALHEIGHLLGLGHST 308
           GTLAHA +P  G  H D  E W +  G +  R        DL++VA+HEIGHLLGLGHS+
Sbjct: 244 GTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSS 303

Query: 309 VKNAIMYPYIKSGSTK-GLNVDDIKGIKVLY 312
           V++AIM+P I  G  K  L  DDI+GI+ LY
Sbjct: 304 VEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

BLAST of CSPI01G32180 vs. TAIR10
Match: AT4G16640.1 (AT4G16640.1 Matrixin family protein)

HSP 1 Score: 189.9 bits (481), Expect = 2.4e-48
Identity = 112/281 (39.86%), Postives = 152/281 (54.09%), Query Frame = 1

Query: 43  GDNVKGISKLKNFFRYYGYLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTG 102
           G +V G+S+LK +   +GY+N              +D+FD  LESAI  YQ+   L  TG
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIF--------SDVFDGPLESAISLYQENLGLPITG 123

Query: 103 SLNAETLSQLATPRCGNPDIINETTGRMLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRW 162
            L+  T++ ++ PRCG  D     T   ++ D             H  +HY +F G+ +W
Sbjct: 124 RLDTSTVTLMSLPRCGVSD-----THMTINNDF-----------LHTTAHYTYFNGKPKW 183

Query: 163 PSTKYRLTYAFLPG------TRADAKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFY 222
              +  LTYA          T  D K    RAF+ W+      F  V ++  ADLKIGFY
Sbjct: 184 --NRDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFY 243

Query: 223 RGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAV-----GAVRGRYDLQTVALHEI 282
            G+HGDG PFDG  GTLAHAFAP +GR H D+ E W V     G+     DL++VA HEI
Sbjct: 244 AGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEI 303

Query: 283 GHLLGLGHSTVKNAIMYPYIKSGSTK-GLNVDDIKGIKVLY 312
           GHLLGLGHS+ ++A+MYP ++  + K  L VDD+ G+  LY
Sbjct: 304 GHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

BLAST of CSPI01G32180 vs. TAIR10
Match: AT2G45040.1 (AT2G45040.1 Matrixin family protein)

HSP 1 Score: 175.3 bits (443), Expect = 6.1e-44
Identity = 107/275 (38.91%), Postives = 138/275 (50.18%), Query Frame = 1

Query: 49  ISKLKNFFRYYGYLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAET 108
           I ++K   + YGYL     +             D   E A+  YQ+   L  TG  +++T
Sbjct: 50  IPEIKRHLQQYGYLPQNKESD------------DVSFEQALVRYQKNLGLPITGKPDSDT 109

Query: 109 LSQLATPRCGNPDIINETTGRMLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWP-STKY 168
           LSQ+  PRCG PD +   T                    H    Y +FPGR RW      
Sbjct: 110 LSQILLPRCGFPDDVEPKTAPF-----------------HTGKKYVYFPGRPRWTRDVPL 169

Query: 169 RLTYAFLPGTRADAKAPV------ARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHG 228
           +LTYAF         AP        RAF  WA      F    +Y  AD+KIGF+ G+HG
Sbjct: 170 KLTYAFSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHG 229

Query: 229 DGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVGAVRGR----YDLQTVALHEIGHLLGL 288
           DG PFDG  G LAH F+P +GR H D  E WAV     +     DL++VA+HEIGH+LGL
Sbjct: 230 DGEPFDGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGL 289

Query: 289 GHSTVKNAIMYPYIKSGSTK-GLNVDDIKGIKVLY 312
           GHS+VK+A MYP +K  S K  LN+DD+ G++ LY
Sbjct: 290 GHSSVKDAAMYPTLKPRSKKVNLNMDDVVGVQSLY 295

BLAST of CSPI01G32180 vs. NCBI nr
Match: gi|449442791|ref|XP_004139164.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 652.1 bits (1681), Expect = 4.9e-184
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 1

Query: 1   MGNKPLAILLLIISIFLPLCFSLPLIQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYG 60
           MGNKPLAILLLIISIFLPLCFSLPLIQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYG
Sbjct: 1   MGNKPLAILLLIISIFLPLCFSLPLIQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYG 60

Query: 61  YLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNP 120
           YLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNP
Sbjct: 61  YLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNP 120

Query: 121 DIINETTGRMLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRAD 180
           DIINETTGRMLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRAD
Sbjct: 121 DIINETTGRMLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRAD 180

Query: 181 AKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPT 240
           AKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPT
Sbjct: 181 AKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPT 240

Query: 241 DGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLN 300
           DGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLN
Sbjct: 241 DGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLN 300

Query: 301 VDDIKGIKVLYNRR 315
           VDDIKGIKVLYNRR
Sbjct: 301 VDDIKGIKVLYNRR 314

BLAST of CSPI01G32180 vs. NCBI nr
Match: gi|659085906|ref|XP_008443669.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 609.4 bits (1570), Expect = 3.6e-171
Identity = 291/314 (92.68%), Postives = 302/314 (96.18%), Query Frame = 1

Query: 1   MGNKPLAILLLIISIFLPLCFSLPLIQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYG 60
           MGNK LAILL I S+FLPLCFSLPL+QVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYG
Sbjct: 1   MGNKTLAILLFI-SVFLPLCFSLPLLQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYG 60

Query: 61  YLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNP 120
           YLNHQINATGHLID DA+D FDDRLE AIKTYQQYFHLNPTGSLNAET+SQLATPRCGNP
Sbjct: 61  YLNHQINATGHLIDTDADDTFDDRLEFAIKTYQQYFHLNPTGSLNAETISQLATPRCGNP 120

Query: 121 DIINETTGRMLSEDIDNVSSHDHHHLPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRAD 180
           DIINE+TGRMLSE  +N SSHDHHHLPHAVSHYAFFPGR RWPSTKYRLTYAFLPGTRAD
Sbjct: 121 DIINESTGRMLSEHNNNDSSHDHHHLPHAVSHYAFFPGRRRWPSTKYRLTYAFLPGTRAD 180

Query: 181 AKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPT 240
           AKAPV RAFATWARNTHFKF+L+TNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPT
Sbjct: 181 AKAPVTRAFATWARNTHFKFSLITNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPT 240

Query: 241 DGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLN 300
           DGRFHYDSTEKWAVGAV+GRYDLQTVALHEIGHLLGLGHSTV+NAIMYPYI+SGSTKGLN
Sbjct: 241 DGRFHYDSTEKWAVGAVKGRYDLQTVALHEIGHLLGLGHSTVRNAIMYPYIRSGSTKGLN 300

Query: 301 VDDIKGIKVLYNRR 315
            DDIKGIKVLYNRR
Sbjct: 301 ADDIKGIKVLYNRR 313

BLAST of CSPI01G32180 vs. NCBI nr
Match: gi|449442789|ref|XP_004139163.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 526.9 bits (1356), Expect = 2.4e-146
Identity = 258/313 (82.43%), Postives = 275/313 (87.86%), Query Frame = 1

Query: 1   MGNKPLAILLLIISIFLPLCFSLPLIQVSPFAFLNDLQGCKKGDNVKGISKLKNFFRYYG 60
           MG+KPLAILL I    LPLCFSLPL QVS  +FLNDLQG KKGDNVKGISKLKNFFR YG
Sbjct: 1   MGSKPLAILLFIS--ILPLCFSLPLRQVSRLSFLNDLQGSKKGDNVKGISKLKNFFRRYG 60

Query: 61  YLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLATPRCGNP 120
           YLNHQIN TGHLID DA+D FDDR ESA+KTYQQYFHLN TGSLNAETLSQLATPRCGNP
Sbjct: 61  YLNHQINVTGHLIDHDADDTFDDRFESAVKTYQQYFHLNSTGSLNAETLSQLATPRCGNP 120

Query: 121 DIINETTGRMLSEDIDNVSSHDHHH-LPHAVSHYAFFPGRLRWPSTKYRLTYAFLPGTRA 180
           DI+NE TGRML E+ +N SSHDH+H L HAV HY+FFPGR RWP TKY LTY FLP T A
Sbjct: 121 DILNEATGRMLLENNNNDSSHDHYHQLSHAVPHYSFFPGRPRWPPTKYHLTYEFLPNTHA 180

Query: 181 DAKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPGGTLAHAFAP 240
           DAKAPV RAFATWAR+THFKF+L TN RRADLKIGFYRGNHGDGYPFDG GGTLAHAF P
Sbjct: 181 DAKAPVTRAFATWARHTHFKFSLATNSRRADLKIGFYRGNHGDGYPFDGSGGTLAHAFTP 240

Query: 241 TDGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPYIKSGSTKGL 300
           TDGR H+DSTEKW VGAVRGR+DL+TVALHEIGHLLGLGHS VKNAIMYP I+SGSTKGL
Sbjct: 241 TDGRVHFDSTEKWVVGAVRGRFDLETVALHEIGHLLGLGHSRVKNAIMYPTIESGSTKGL 300

Query: 301 NVDDIKGIKVLYN 313
           N DDI+GI+VLYN
Sbjct: 301 NADDIEGIEVLYN 311

BLAST of CSPI01G32180 vs. NCBI nr
Match: gi|255552736|ref|XP_002517411.1| (PREDICTED: metalloendoproteinase 3-MMP [Ricinus communis])

HSP 1 Score: 346.7 bits (888), Expect = 4.4e-92
Identity = 179/322 (55.59%), Postives = 224/322 (69.57%), Query Frame = 1

Query: 1   MGNKPLAILLLIISIFLPLCFSLPLI-------QVSPFAFLNDLQGCKKGDNVKGISKLK 60
           M +KP AI  + + + + L  S  L        + S F FL  LQGC KGDN+KGI  LK
Sbjct: 1   MASKPFAIFSVTLILLISLLSSAALAHSHSKTEKSSAFDFLKHLQGCHKGDNLKGIHDLK 60

Query: 61  NFFRYYGYLNHQINATGHLIDIDANDIFDDRLESAIKTYQQYFHLNPTGSLNAETLSQLA 120
            +   +GYL+++  +  +      +D FDD LE A+KTYQ  +HLN TG L++ET++++ 
Sbjct: 61  KYLENFGYLSYKNQSHSN------DDDFDDLLEYALKTYQFNYHLNVTGFLDSETVTKMM 120

Query: 121 TPRCGNPDIINETTGRMLSEDIDNVSSHDHHHLP---HAVSHYAFFPGRLRWPSTKYRLT 180
            PRCG  DIIN TT RM S +      + HHH     H VSHY FFPG  RWP++KY LT
Sbjct: 121 MPRCGVADIINGTT-RMQSSN-----KNPHHHSSTSFHTVSHYEFFPGNPRWPASKYHLT 180

Query: 181 YAFLPGTRADAKAPVARAFATWARNTHFKFTLVTNYRRADLKIGFYRGNHGDGYPFDGPG 240
           Y FLPGT   A  PVA+AF TWA NTHF+FT V +YR AD+ IGF+RG+HGDG PFDG G
Sbjct: 181 YGFLPGTPNQAMEPVAKAFQTWAANTHFRFTRVQDYRAADITIGFHRGDHGDGSPFDGRG 240

Query: 241 GTLAHAFAPTDGRFHYDSTEKWAVGAVRGRYDLQTVALHEIGHLLGLGHSTVKNAIMYPY 300
           GTLAHAFAP DGRFHYD  E WAVGA +G +D++TVALHEIGHLLGLGHS+V+ AIM+P 
Sbjct: 241 GTLAHAFAPQDGRFHYDGDEHWAVGATQGAFDVETVALHEIGHLLGLGHSSVEGAIMHPS 300

Query: 301 IKSGSTKGLNVDDIKGIKVLYN 313
           I+SG+TKGL+ DDI+GI+ LYN
Sbjct: 301 IQSGATKGLHSDDIQGIRALYN 310

BLAST of CSPI01G32180 vs. NCBI nr
Match: gi|645249271|ref|XP_008230675.1| (PREDICTED: metalloendoproteinase 1-like [Prunus mume])

HSP 1 Score: 340.9 bits (873), Expect = 2.4e-90
Identity = 170/284 (59.86%), Postives = 204/284 (71.83%), Query Frame = 1

Query: 29  SPFAFLNDLQGCKKGDNVKGISKLKNFFRYYGYLNHQINATGHLIDIDANDIFDDRLESA 88
           SPF FL  L+GC KGD V+GI  LK +   +GYL+   N  GH  D D    FDD+LESA
Sbjct: 32  SPFEFLEHLKGCHKGDKVQGIQDLKKYLGKFGYLSS--NNNGHFNDDD----FDDQLESA 91

Query: 89  IKTYQQYFHLNPTGSLNAETLSQLATPRCGNPDIINETTGRMLSEDIDNVSSHDHHHLPH 148
           IKTYQ  +HL  TG+L+AET+S +  PRCG  DIIN T+     +       H HHH  H
Sbjct: 92  IKTYQLNYHLKATGTLDAETVSNMMMPRCGVADIINGTSSMRSGK---QRHPHHHHHGGH 151

Query: 149 AVSHYAFFPGRLRWPSTKYRLTYAFLPGTRADAKAPVARAFATWARNTHFKFTLVTNYRR 208
            V+HY FF G  +WP++KY LTYAFL GT A+A  PVARAF TWA NTHF F+   + + 
Sbjct: 152 TVAHYTFFRGNPKWPASKYHLTYAFLQGTPAEATGPVARAFQTWAANTHFTFS--QSNQN 211

Query: 209 ADLKIGFYRGNHGDGYPFDGPGGTLAHAFAPTDGRFHYDSTEKWAVGAVRGRYDLQTVAL 268
            DL + F+RGNHGDG PFDGPGGT+AHAFAPT+GRFHYD+ E+++VGAV G YDL+TVAL
Sbjct: 212 PDLTVSFHRGNHGDGSPFDGPGGTIAHAFAPTNGRFHYDADERFSVGAVSGAYDLETVAL 271

Query: 269 HEIGHLLGLGHSTVKNAIMYPYIKSGSTKGLNVDDIKGIKVLYN 313
           HEIGHLLGLGHS+V  AIMYP I  G+TKGL+ DDI+GIK LYN
Sbjct: 272 HEIGHLLGLGHSSVLGAIMYPTISPGATKGLHGDDIQGIKALYN 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
3MMP_ARATH4.7e-6243.99Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1[more]
2MMP_ARATH3.0e-6142.61Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1[more]
5MMP_ARATH2.9e-5943.20Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1[more]
MEP1_SOYBN5.6e-5541.88Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2[more]
1MMP_ARATH4.3e-4739.86Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0M0J6_CUCSA3.4e-184100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G654860 PE=3 SV=1[more]
A0A0A0M032_CUCSA1.6e-14682.43Uncharacterized protein OS=Cucumis sativus GN=Csa_1G654840 PE=3 SV=1[more]
B9RUG6_RICCO3.0e-9255.59Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1[more]
S1SMU3_THECC2.4e-8956.60Matrixin family protein OS=Theobroma cacao GN=TCM_044196 PE=3 SV=1[more]
A0A061FPL5_THECC9.2e-8956.29Matrixin family protein OS=Theobroma cacao GN=TCM_044201 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G24140.12.6e-6343.99 Matrixin family protein[more]
AT1G70170.11.7e-6242.61 matrix metalloproteinase[more]
AT1G59970.11.6e-6043.20 Matrixin family protein[more]
AT4G16640.12.4e-4839.86 Matrixin family protein[more]
AT2G45040.16.1e-4438.91 Matrixin family protein[more]
Match NameE-valueIdentityDescription
gi|449442791|ref|XP_004139164.1|4.9e-184100.00PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|659085906|ref|XP_008443669.1|3.6e-17192.68PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
gi|449442789|ref|XP_004139163.1|2.4e-14682.43PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|255552736|ref|XP_002517411.1|4.4e-9255.59PREDICTED: metalloendoproteinase 3-MMP [Ricinus communis][more]
gi|645249271|ref|XP_008230675.1|2.4e-9059.86PREDICTED: metalloendoproteinase 1-like [Prunus mume][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001818Pept_M10_metallopeptidase
IPR002477Peptidoglycan-bd-like
IPR006026Peptidase_Metallo
IPR021158Pept_M10A_Zn_BS
IPR021190Pept_M10A
IPR024079MetalloPept_cat_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004222metalloendopeptidase activity
GO:0008270zinc ion binding
GO:0008237metallopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Cellular Component
TermDefinition
GO:0031012extracellular matrix
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G32180.1CSPI01G32180.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 166..311
score: 4.2
IPR002477Peptidoglycan binding-likeGENE3DG3DSA:1.10.101.10coord: 49..114
score: 2.8
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 50..112
score: 3.
IPR002477Peptidoglycan binding-likeunknownSSF47090PGBD-likecoord: 41..122
score: 1.73
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 158..313
score: 7.4
IPR021158Peptidase M10A, cysteine switch, zinc binding sitePROSITEPS00546CYSTEINE_SWITCHcoord: 115..122
scor
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 299..312
score: 2.6E-35coord: 209..237
score: 2.6E-35coord: 112..125
score: 2.6E-35coord: 185..200
score: 2.6E-35coord: 266..291
score: 2.6
IPR024079Metallopeptidase, catalytic domainGENE3DG3DSA:3.40.390.10coord: 115..312
score: 1.9
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 11..128
score: 2.5E-124coord: 148..311
score: 2.5E
NoneNo IPR availablePANTHERPTHR10201:SF140F20P5.11 PROTEINcoord: 11..128
score: 2.5E-124coord: 148..311
score: 2.5E
NoneNo IPR availableunknownSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 155..312
score: 1.2

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None