CSPI01G27750 (gene) Wild cucumber (PI 183967)

NameCSPI01G27750
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionIAA-amino acid hydrolase ILR1, putative
LocationChr1 : 22556333 .. 22560027 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTGAGAAAACTCAATTTTAACTCGGATATGTTTTCTTTAAAATTCAACAGCTTACTTCAACTCATAATCGAGAGTATAGCATTTGGAAATGACTTTAGATCTTTGATTCATAGCAAAAATCAAACATAGAATAATGTTCTCAATTTGCCCAAAAGAAGGAAGGAAGGAAGAAAACATACGCTCATTGGTTGGGAGAATATTCTCTTTCGTTGAGCTAAGTTGGTGCCAAGAGGTCATTCTTTCCACAGTGAGTCTCATCACTAAATACCCATTTCCATTACTCTCAATCATTCCACCTTCTCAAATTACAACCAAATCAATGCCTCAACGCAGTTTCTTGAATTTGATCCTCATTTTCCATCTCCTCATCTCACTGTTAATTCCTTCGAGTTCTTCTCCAATCCCAGATGAGTTATCTCAAATCCCTTCGTTTTTCCTTCAATATGCTCAACATCCAGAGGTTTTTGATTGGATGGTTGGTATCAGAAGGAAAATACACGAAAACCCTGAATTGGGTTTCCAGGAATTTGAGACTAGCAAGCTTATCAGAGCGGAGCTTGACCGTTTAGGCATTTCTTATGAATATCCAGTGGCCATCACCGGCGTCGTTGGTTTCATCGGAACTGGAAATCCTCCTTTCGTTGCTATTCGTGCAGACATGGATGCTCTACCCCTCCAGGTATACCCCAAAACTTCTTAATCTCAGTCTTCAATTCTGTTTCTTTTGTTTGATTGTGATTGTGGGGATGGTTTTGAAACTGAAAACTCAATGGATTTCATGTTACATGATTATAAAATACGAAACCTAAAAGAGAATATACAACCCCAATGAATTCAAGTTTCATCTTTAACACAATTTTACTTACCTTAGTGCTGAGTTGGGAATAATTGTGTTGCCAATAATTGTCTCACAGGATACAATAGATTAGTTTTCCTATGAAAGCAGTACAATTGTCCACAAGATCCGACCATCTTCACTTTTGTAGAGTAAACCAAACTTCAACAAATTTTTAAATGGAAGGCAAGTTTTATTAGAAAAGTGTTGACATATTTATAGACTAAGAGTGTATTTGGTTTAACTTTTCAAGTATTAATTTTAAAAACATATCATTTTTAGAAGAATTGAAGTTGTTGACCATCACTCAACATAGCTCTTTAAACACTCTCAAAGTGTAATCTTAAATAGTTTTTACTTTTTTTTCCCTAATCAATCCGAACATATCATAATTTGTGGTTATTTGAGTAGTCGTATCTCTTTTTCAAATTTGTTAAAAAATTGAAAAGGTGTATCCTTTGAAGAGTTACATACCCATTTATTTCAACATTTCACCTGTTGTTGCATACATGCCCTTTCATTGAACTTTGAGAGAAATTATTTGTGTAATGTTGGTTGTAATATAGGAGGGTGTTGAATGGGAACACAAAAGCAAAGTTCCTGGAAAGATGCATGCATGCGGCCATGATGCTCATGTTGCTATGCTTCTAGGTGCTGCAAAGATTCTTCAACAGAACAGCCGTCTAATACAGGTTCTTTTTCTTCCTCTTGAAATTTGTGTAGTTTGGATATTGATATGGAGATTAATATCAAGCCCCCAGCAATCCAATCAACACCTCAAATTCATGCACCTGAACCTATAGCAACACGCTCTCTTTTTTTACGACACTGGAATCCCCGACTCAATAACGGTTGAGAAAAAACCTCCCACTACTACCATAGGAGTAAAGAAAAAATAACATAAAAGCAATAAAACTAGGAACACAAGAATTAACATGGAAAACTCTATCCCAAAGGAAAAAACCATGGACTAAAAAGAAATTCCATGGAGAAAATTTGTTACAATCACACAAAAAGCACTCCCCGACCCCAATCACAAAGAAAACACTCTCTTAAAGCATTATATCAATCACACAAATGAGTTAGTCAGTCAAGCTTATCATGCTTCAATTTGGGATGACTTGAAACCAAAATCACTGGCTGTAAAGTTTTTACACTCTCTCAGCATGATGTTTATGCTTATGACTGGCTTAGATATTGTTCATCCTGCTATTCTAAATTTTGGTGTTGAGGTCCTAATTTTAACAGGGAACAGTAGTTCTTGTTTTTCAACCAGCTGAAGAAGGAGGTGGGGGAGCAAAGAAAATGTTAGAGGCTGGAGTACTGGATAAAGTTGATGCCATTTTTGGTTTACATGTAAGTTCCAGTTATCCTTTTGGTATGGCGATCTCAAAGCCTGGTCCTATTCTGGCTGCAAGTGGTTTCTTTGAGGCAGTTATTGGTGGTAAGGGAGGTCATGCTGCTCTTCCTCAACACACAATTGACCCAATTTTAGCAGCTTCCAACATAATTGTCAGTTTGCAACATCTCGTCTCGCGCGAAGCCGATCCATTGGATTCACAGGTACTTTTCCTTTTAAAAATTTCTCGGGTGTACAAGAAGAATCAGATCTCGTTATTAAAATGCTCTGAGTTTGAGAGCACTCCAACGGTAGTCCGAGTAAGAGTTGGAGAGTAGAGTTACTGCTTGTTTAGCTTAAAAGATAGGAGTTTCAAAGGTTTAGGCTGGATTAAGGTTGAGTTTTATATGTTTTCTAGGCTATTAAAAAGCACGTTTTACCAATCAAACAACTCCCAAATTTCTTTGCTGAATTCGTTTTTCAAAAGTCATTGTAGGCTTACCATTATTCTACATGGATTTTGGCTTTTCTTCCTAATTTTCAATTTGGCAAAGAACCACTATGATTGCTTACTTTTATAATTTAGTTAAGATGTTTAAACATATTTGAATCTTTTAGGTCGTTACAATAGCAAAGTTTCAAGGCGGTGGTGCATTTAATGTCATACCAGATTCTGTTACCATTGGAGGCACTTTCAGGGCATTTTCAAAAGAAAGCTTCATTCAACTTAAACAAAGAATTGTAGAGGTATCTATCTTACAAGAAATATATATTTCCTTTACGTCAAAATTCATTCTCATGATGATTCTTTCGTCTTGGAATTAACAATGCCAGGTTATCACAAATCAAGCCAAAGTTCAAAGGTGCAATGCCACTGTTGAGTTCAATGAAGATCAAAAGCCTTTTTTTCCAGTTACAGTAAATAACCAAAATCTTTACAAGCATTTTCGAACAGTCGCGGTTGACATGTTAGGTACTAAGAACATTCTTGATATGCAACCGTTAATGGGAGCAGAAGACTTTGGATTCTACGGTGAAGTGATTCCAGGATTGTTCTACTTTCTTGGGATGAAAAATGAAACTATAGGAAAGTTTGAACAAGGACATTCACCTTATTATATTGTAAATGAAGATGTTCTTCCCTATGGTGCAGCGTTGCAGGCATCAATCGCCACAAGGTACCTTCTAGAGCACTCACCTAAATCTACTTCAACTAAAGAGCACTCTCATGATGAGCTTTAACGATTTGTTGTTGTGGGATAGGAATGTCGTTAGTTGTTAGTAATTAGCTAATAGAGGGTGAGATATGAGAAAGGAGAATGTTTTGTTGTAGTTAGTGAACTATTGCATGATTTATCTCCTAATTTCTTTATCGTGTATTTCAATAAATTAAAAATCTATCCATGTATAGTGTCATTCAAGTTAAGCTCTTTTTGAAGTGAATCTATCACTGTATAGTGTCATCCAAGTATTGACCCTTTTCTGCAGTCGTTCTTTGATGTGATTATCATCTCAATAT

mRNA sequence

ATGTTCTCAATTTGCCCAAAAGAAGGAAGGAAGGAAGAAAACATACGCTCATTGGTTGGGAGAATATTCTCTTTCGTTGAGCTAAGTTGGTGCCAAGAGGTCATTCTTTCCACAGTGAGTCTCATCACTAAATACCCATTTCCATTACTCTCAATCATTCCACCTTCTCAAATTACAACCAAATCAATGCCTCAACGCAGTTTCTTGAATTTGATCCTCATTTTCCATCTCCTCATCTCACTGTTAATTCCTTCGAGTTCTTCTCCAATCCCAGATGAGTTATCTCAAATCCCTTCGTTTTTCCTTCAATATGCTCAACATCCAGAGGTTTTTGATTGGATGGTTGGTATCAGAAGGAAAATACACGAAAACCCTGAATTGGGTTTCCAGGAATTTGAGACTAGCAAGCTTATCAGAGCGGAGCTTGACCGTTTAGGCATTTCTTATGAATATCCAGTGGCCATCACCGGCGTCGTTGGTTTCATCGGAACTGGAAATCCTCCTTTCGTTGCTATTCGTGCAGACATGGATGCTCTACCCCTCCAGGAGGGTGTTGAATGGGAACACAAAAGCAAAGTTCCTGGAAAGATGCATGCATGCGGCCATGATGCTCATGTTGCTATGCTTCTAGGTGCTGCAAAGATTCTTCAACAGAACAGCCGTCTAATACAGGGAACAGTAGTTCTTGTTTTTCAACCAGCTGAAGAAGGAGGTGGGGGAGCAAAGAAAATGTTAGAGGCTGGAGTACTGGATAAAGTTGATGCCATTTTTGGTTTACATGTAAGTTCCAGTTATCCTTTTGGTATGGCGATCTCAAAGCCTGGTCCTATTCTGGCTGCAAGTGGTTTCTTTGAGGCAGTTATTGGTGGTAAGGGAGGTCATGCTGCTCTTCCTCAACACACAATTGACCCAATTTTAGCAGCTTCCAACATAATTGTCAGTTTGCAACATCTCGTCTCGCGCGAAGCCGATCCATTGGATTCACAGGTCGTTACAATAGCAAAGTTTCAAGGCGGTGGTGCATTTAATGTCATACCAGATTCTGTTACCATTGGAGGCACTTTCAGGGCATTTTCAAAAGAAAGCTTCATTCAACTTAAACAAAGAATTGTAGAGGTTATCACAAATCAAGCCAAAGTTCAAAGGTGCAATGCCACTGTTGAGTTCAATGAAGATCAAAAGCCTTTTTTTCCAGTTACAGTAAATAACCAAAATCTTTACAAGCATTTTCGAACAGTCGCGGTTGACATGTTAGGTACTAAGAACATTCTTGATATGCAACCGTTAATGGGAGCAGAAGACTTTGGATTCTACGGTGAAGTGATTCCAGGATTGTTCTACTTTCTTGGGATGAAAAATGAAACTATAGGAAAGTTTGAACAAGGACATTCACCTTATTATATTGTAAATGAAGATGTTCTTCCCTATGGTGCAGCGTTGCAGGCATCAATCGCCACAAGGTACCTTCTAGAGCACTCACCTAAATCTACTTCAACTAAAGAGCACTCTCATGATGAGCTTTAA

Coding sequence (CDS)

ATGTTCTCAATTTGCCCAAAAGAAGGAAGGAAGGAAGAAAACATACGCTCATTGGTTGGGAGAATATTCTCTTTCGTTGAGCTAAGTTGGTGCCAAGAGGTCATTCTTTCCACAGTGAGTCTCATCACTAAATACCCATTTCCATTACTCTCAATCATTCCACCTTCTCAAATTACAACCAAATCAATGCCTCAACGCAGTTTCTTGAATTTGATCCTCATTTTCCATCTCCTCATCTCACTGTTAATTCCTTCGAGTTCTTCTCCAATCCCAGATGAGTTATCTCAAATCCCTTCGTTTTTCCTTCAATATGCTCAACATCCAGAGGTTTTTGATTGGATGGTTGGTATCAGAAGGAAAATACACGAAAACCCTGAATTGGGTTTCCAGGAATTTGAGACTAGCAAGCTTATCAGAGCGGAGCTTGACCGTTTAGGCATTTCTTATGAATATCCAGTGGCCATCACCGGCGTCGTTGGTTTCATCGGAACTGGAAATCCTCCTTTCGTTGCTATTCGTGCAGACATGGATGCTCTACCCCTCCAGGAGGGTGTTGAATGGGAACACAAAAGCAAAGTTCCTGGAAAGATGCATGCATGCGGCCATGATGCTCATGTTGCTATGCTTCTAGGTGCTGCAAAGATTCTTCAACAGAACAGCCGTCTAATACAGGGAACAGTAGTTCTTGTTTTTCAACCAGCTGAAGAAGGAGGTGGGGGAGCAAAGAAAATGTTAGAGGCTGGAGTACTGGATAAAGTTGATGCCATTTTTGGTTTACATGTAAGTTCCAGTTATCCTTTTGGTATGGCGATCTCAAAGCCTGGTCCTATTCTGGCTGCAAGTGGTTTCTTTGAGGCAGTTATTGGTGGTAAGGGAGGTCATGCTGCTCTTCCTCAACACACAATTGACCCAATTTTAGCAGCTTCCAACATAATTGTCAGTTTGCAACATCTCGTCTCGCGCGAAGCCGATCCATTGGATTCACAGGTCGTTACAATAGCAAAGTTTCAAGGCGGTGGTGCATTTAATGTCATACCAGATTCTGTTACCATTGGAGGCACTTTCAGGGCATTTTCAAAAGAAAGCTTCATTCAACTTAAACAAAGAATTGTAGAGGTTATCACAAATCAAGCCAAAGTTCAAAGGTGCAATGCCACTGTTGAGTTCAATGAAGATCAAAAGCCTTTTTTTCCAGTTACAGTAAATAACCAAAATCTTTACAAGCATTTTCGAACAGTCGCGGTTGACATGTTAGGTACTAAGAACATTCTTGATATGCAACCGTTAATGGGAGCAGAAGACTTTGGATTCTACGGTGAAGTGATTCCAGGATTGTTCTACTTTCTTGGGATGAAAAATGAAACTATAGGAAAGTTTGAACAAGGACATTCACCTTATTATATTGTAAATGAAGATGTTCTTCCCTATGGTGCAGCGTTGCAGGCATCAATCGCCACAAGGTACCTTCTAGAGCACTCACCTAAATCTACTTCAACTAAAGAGCACTCTCATGATGAGCTTTAA
BLAST of CSPI01G27750 vs. Swiss-Prot
Match: ILL4_ARATH (IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsis thaliana GN=ILL4 PE=1 SV=2)

HSP 1 Score: 599.7 bits (1545), Expect = 2.9e-170
Identity = 297/440 (67.50%), Postives = 362/440 (82.27%), Query Frame = 1

Query: 68  FLNLILIFHLLISLLIPSSSSPIPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIHENPEL 127
           +++ +LI HLL   LI  SS+     LSQIPS FL  A+  + FDWMVGIRR+IHENPEL
Sbjct: 6   WVSFVLILHLLNPTLISCSSNG----LSQIPSKFLTLAKRNDFFDWMVGIRRRIHENPEL 65

Query: 128 GFQEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQEGVEW 187
           G++E ETSKL+RAEL+++G+SY+YPVA+TGVVG++GTG+ PFVA+RADMDAL +QE VEW
Sbjct: 66  GYEEVETSKLVRAELEKMGVSYKYPVAVTGVVGYVGTGHAPFVALRADMDALAMQEMVEW 125

Query: 188 EHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAKKMLEA 247
           EHKSKVPGKMHACGHDAH  MLLGAAK+L+++   +QGTVVLVFQPAEEGGGGAKK++EA
Sbjct: 126 EHKSKVPGKMHACGHDAHTTMLLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAKKIVEA 185

Query: 248 GVLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTIDPILA 307
           GVL+ V AIFGLHV++    G   S+ GP+LA SGFF+A I GKGGHAALPQHTIDPILA
Sbjct: 186 GVLENVSAIFGLHVTNQLALGQVSSREGPMLAGSGFFKAKISGKGGHAALPQHTIDPILA 245

Query: 308 ASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLK 367
           ASN+IVSLQHLVSREADPLDSQVVT+AKF+GGGAFNVIPDSVTIGGTFRAFS +SF+QLK
Sbjct: 246 ASNVIVSLQHLVSREADPLDSQVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKSFMQLK 305

Query: 368 QRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNQNLYKHFRTVAVDMLGTKNILDMQ 427
           +RI +VIT QA V  CNATV+F E++KPFFP TVN++ L++ F+ V+ DMLG +N ++MQ
Sbjct: 306 KRIEQVITRQASVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIENYVEMQ 365

Query: 428 PLMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQASIATR 487
           PLMG+EDF FY + IPG F F+GM+N+        HSPY+ VNE++LPYGA+L AS+ATR
Sbjct: 366 PLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHASMATR 425

Query: 488 YLLEHSPKSTSTKEHSHDEL 508
           YLLE    ST  K +  DEL
Sbjct: 426 YLLE-LKASTLNKSNKKDEL 440

BLAST of CSPI01G27750 vs. Swiss-Prot
Match: ILL1_ORYSI (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica GN=ILL1 PE=2 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 1.3e-165
Identity = 283/411 (68.86%), Postives = 334/411 (81.27%), Query Frame = 1

Query: 98  PSFFLQYAQHPEVFDWMVGIRRKIHENPELGFQEFETSKLIRAELDRLGISYEYPVAITG 157
           P+  L+ A+  E   WMVG+RR+IHENPELG++EF TS+L+R ELD LGI Y +P A+TG
Sbjct: 33  PAGLLRRAKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTG 92

Query: 158 VVGFIGTGNPPFVAIRADMDALPLQEGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQ 217
           VV  +GTG PPFVA+RADMDALP+QE VEWEHKSKVPGKMH CGHDAHVAMLLG+A+ILQ
Sbjct: 93  VVATVGTGGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQ 152

Query: 218 QNSRLIQGTVVLVFQPAEEGGGGAKKMLEAGVLDKVDAIFGLHVSSSYPFGMAISKPGPI 277
           ++   ++GTVVLVFQPAEEGGGGAKKM++ G ++ ++AIFG+HV+   P G+  S+PGP+
Sbjct: 153 EHRDELKGTVVLVFQPAEEGGGGAKKMIDDGTVENIEAIFGVHVADVVPIGVVASRPGPV 212

Query: 278 LAASGFFEAVIGGKGGHAALPQHTIDPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQ 337
           +A SGFFEAVI GKGGHAALP HTIDPILAASN+IVSLQ LVSREADPLDSQVVT+ KFQ
Sbjct: 213 MAGSGFFEAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQ 272

Query: 338 GGGAFNVIPDSVTIGGTFRAFSKESFIQLKQRIVEVITNQAKVQRCNATVEFNEDQKPFF 397
           GGGAFNVIPDSVTIGGTFRAF KESF QLKQRI EVI +QA VQRCNA V+F +  +PFF
Sbjct: 273 GGGAFNVIPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFF 332

Query: 398 PVTVNNQNLYKHFRTVAVDMLGTKNILDMQPLMGAEDFGFYGEVIPG-LFYFLGMKNETI 457
           P T+N+  L+  F  VA +M+G KN+ D QPLMGAEDF FY + IP   +YFLGM NET 
Sbjct: 333 PPTINSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETR 392

Query: 458 GKFEQGHSPYYIVNEDVLPYGAALQASIATRYLLEHSPKSTSTKEHSHDEL 508
           G     HSPY+ +NED LPYGAALQAS+ATRYLLEH P +T  K  +HDEL
Sbjct: 393 GPQAPHHSPYFTINEDALPYGAALQASLATRYLLEHQPPTTG-KAKAHDEL 442

BLAST of CSPI01G27750 vs. Swiss-Prot
Match: ILL1_ORYSJ (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica GN=ILL1 PE=2 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 4.8e-165
Identity = 282/411 (68.61%), Postives = 333/411 (81.02%), Query Frame = 1

Query: 98  PSFFLQYAQHPEVFDWMVGIRRKIHENPELGFQEFETSKLIRAELDRLGISYEYPVAITG 157
           P+  L+ A+  E   WMVG+RR+IHENPELG++EF TS+L+R ELD LGI Y +P A+TG
Sbjct: 33  PAGLLRRAKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTG 92

Query: 158 VVGFIGTGNPPFVAIRADMDALPLQEGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQ 217
           VV  +GTG PPFVA+RADMDALP+QE VEWEHKSKVPGKMH CGHDAHVAMLLG+A+ILQ
Sbjct: 93  VVATVGTGGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQ 152

Query: 218 QNSRLIQGTVVLVFQPAEEGGGGAKKMLEAGVLDKVDAIFGLHVSSSYPFGMAISKPGPI 277
           ++   ++GTVVLVFQPAEEGGGGAKKM++ G ++ ++AIFG+HV+   P G+  S+PGP+
Sbjct: 153 EHRDELKGTVVLVFQPAEEGGGGAKKMIDDGAVENIEAIFGVHVADVVPIGVVASRPGPV 212

Query: 278 LAASGFFEAVIGGKGGHAALPQHTIDPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQ 337
           +A SGFFEAVI GKGGHAALP HTIDPILAASN+IVSLQ LVSREADPLDSQVVT+ KFQ
Sbjct: 213 MAGSGFFEAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQ 272

Query: 338 GGGAFNVIPDSVTIGGTFRAFSKESFIQLKQRIVEVITNQAKVQRCNATVEFNEDQKPFF 397
           GGGAFNVIPDSVTIGGTFRAF KESF QLKQRI EVI +QA VQRCNA V+F +  +PFF
Sbjct: 273 GGGAFNVIPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFF 332

Query: 398 PVTVNNQNLYKHFRTVAVDMLGTKNILDMQPLMGAEDFGFYGEVIPG-LFYFLGMKNETI 457
           P T+N+  L+  F  VA +M+G KN+ D QPLMGAEDF FY + IP   +YFLGM NET 
Sbjct: 333 PPTINSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETR 392

Query: 458 GKFEQGHSPYYIVNEDVLPYGAALQASIATRYLLEHSPKSTSTKEHSHDEL 508
           G     HSPY+ +NED LPYGAALQAS+A RYLLEH P +T  K  +HDEL
Sbjct: 393 GPQAPHHSPYFTINEDALPYGAALQASLAARYLLEHQPPTTG-KAKAHDEL 442

BLAST of CSPI01G27750 vs. Swiss-Prot
Match: ILL5_ARATH (IAA-amino acid hydrolase ILR1-like 5 OS=Arabidopsis thaliana GN=ILL5 PE=3 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 2.8e-157
Identity = 272/425 (64.00%), Postives = 341/425 (80.24%), Query Frame = 1

Query: 69  LNLILIFHLLISLLIPSSSSPIPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIHENPELG 128
           ++ +LI HLL S LI  SS+    +LSQIP  FL  A+  + FDWMVGIRR+IHENPELG
Sbjct: 7   VSFVLILHLLNSCLISCSSN----DLSQIPKNFLSLAKREDFFDWMVGIRRRIHENPELG 66

Query: 129 FQEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQEGVEWE 188
           ++E ETSKL++ ELD++G+SY+ PVA+TGV+G++GTG+ PFVA+RADMDALP+QE VEWE
Sbjct: 67  YEEVETSKLVKTELDKMGVSYKNPVAVTGVIGYVGTGHAPFVALRADMDALPIQEMVEWE 126

Query: 189 HKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAKKMLEAG 248
           HKSK+PGKMHACGHDAH  MLLGAAK+L+++   +QGTV+LVFQPAEEGG GAKK++EAG
Sbjct: 127 HKSKIPGKMHACGHDAHTTMLLGAAKLLKEHQEELQGTVILVFQPAEEGGAGAKKIVEAG 186

Query: 249 VLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTIDPILAA 308
           VL+ V AIFGLHVS+    G   S+ G ++A SG F+A I GKGGHAALPQ  IDP+LAA
Sbjct: 187 VLENVGAIFGLHVSNLLGLGQLSSREGLLMAGSGRFKATISGKGGHAALPQFAIDPVLAA 246

Query: 309 SNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLKQ 368
           SN+I+SLQHLVSREADPLDSQVVT+A F+G  AFNVIPDSVTIGGTFRA   +SF QLKQ
Sbjct: 247 SNVILSLQHLVSREADPLDSQVVTVATFEGSDAFNVIPDSVTIGGTFRALLPKSFEQLKQ 306

Query: 369 RIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNQNLYKHFRTVAVDMLGTKNILDMQP 428
           RIV+VIT QA V  CNATV+F ED+ P FP TVNN+ L+  ++ V+VDMLG +N ++  P
Sbjct: 307 RIVQVITTQASVNMCNATVDFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIENYVETLP 366

Query: 429 LMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQASIATRY 488
           +M +EDF FY + IPG F F+GM+N++       HSP++ VNE++LPYGA+L AS+ATRY
Sbjct: 367 VMVSEDFAFYQQAIPGHFSFVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLASLATRY 426

Query: 489 LLEHS 494
           LL+ S
Sbjct: 427 LLDSS 427

BLAST of CSPI01G27750 vs. Swiss-Prot
Match: ILL2_ARATH (IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsis thaliana GN=ILL2 PE=1 SV=2)

HSP 1 Score: 542.7 bits (1397), Expect = 4.2e-153
Identity = 271/433 (62.59%), Postives = 335/433 (77.37%), Query Frame = 1

Query: 71  LILIFHLLISLLIPSSSSP-IPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIHENPELGF 130
           L L F LL+ LL  SS SP I ++ SQI +  L++A+ PEVFDWMV IRRKIHENPELG+
Sbjct: 7   LSLTFQLLLFLLSVSSESPWIAEDTSQIQTKLLEFAKSPEVFDWMVKIRRKIHENPELGY 66

Query: 131 QEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQEGVEWEH 190
           +E ETSKLIR+EL+ +GI Y YPVAITGV+G+IGTG PPFVA+RADMDALP+QEGVEWEH
Sbjct: 67  EELETSKLIRSELELIGIKYRYPVAITGVIGYIGTGEPPFVALRADMDALPIQEGVEWEH 126

Query: 191 KSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAKKMLEAGV 250
           KSK+ GKMHACGHD HV MLLGAAKIL ++   +QGTVVL+FQPAEEG  GAKKM E G 
Sbjct: 127 KSKIAGKMHACGHDGHVTMLLGAAKILHEHRHHLQGTVVLIFQPAEEGLSGAKKMREEGA 186

Query: 251 LDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTIDPILAAS 310
           L  V+AIFG+H+S+  PFG A S+ G  LA +G FEAVI GKGGHAA+PQHTIDP++AAS
Sbjct: 187 LKNVEAIFGIHLSARIPFGKAASRAGSFLAGAGVFEAVITGKGGHAAIPQHTIDPVVAAS 246

Query: 311 NIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLKQR 370
           +I++SLQ LVSRE DPLDS+VVT++K  GG AFNVIPDS+TIGGT RAF+   F QL+QR
Sbjct: 247 SIVLSLQQLVSRETDPLDSKVVTVSKVNGGNAFNVIPDSITIGGTLRAFT--GFTQLQQR 306

Query: 371 IVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNQNLYKHFRTVAVDMLGTKNILDMQPL 430
           + EVIT QA V RCNA+V    + +   P TVNN++LYK F+ V  D+LG +  ++  P+
Sbjct: 307 VKEVITKQAAVHRCNASVNLTPNGREPMPPTVNNKDLYKQFKKVVRDLLGQEAFVEAAPV 366

Query: 431 MGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQASIATRYL 490
           MG+EDF ++ E IPG F  LGM++ET G +   HSP Y +NEDVLPYGAA+ AS+A +YL
Sbjct: 367 MGSEDFSYFAETIPGHFSLLGMQDETNG-YASSHSPLYRINEDVLPYGAAIHASMAVQYL 426

Query: 491 LEHSPKSTSTKEH 503
            E + K + +  H
Sbjct: 427 KEKASKGSVSGFH 436

BLAST of CSPI01G27750 vs. TrEMBL
Match: A0A0A0LYS1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G575150 PE=4 SV=1)

HSP 1 Score: 885.9 bits (2288), Expect = 2.2e-254
Identity = 441/445 (99.10%), Postives = 444/445 (99.78%), Query Frame = 1

Query: 63  MPQRSFLNLILIFHLLISLLIPSSSSPIPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIH 122
           MPQRSFLNLILIFH+LISLLIPSSSSPIPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIH
Sbjct: 1   MPQRSFLNLILIFHILISLLIPSSSSPIPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIH 60

Query: 123 ENPELGFQEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQ 182
           ENPELGFQEFETSKLIRAELDRLG+SYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQ
Sbjct: 61  ENPELGFQEFETSKLIRAELDRLGVSYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQ 120

Query: 183 EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAK 242
           EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAK
Sbjct: 121 EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAK 180

Query: 243 KMLEAGVLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTI 302
           KMLEAGVLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTI
Sbjct: 181 KMLEAGVLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTI 240

Query: 303 DPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKES 362
           DPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKES
Sbjct: 241 DPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300

Query: 363 FIQLKQRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNQNLYKHFRTVAVDMLGTKN 422
           FIQLKQRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNN NLYKHFRTVAVDMLGTKN
Sbjct: 301 FIQLKQRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNHNLYKHFRTVAVDMLGTKN 360

Query: 423 ILDMQPLMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQA 482
           ILDMQPLMGAEDFGFYGEVIPGLFYFLGMKNET+GKFEQGHSPYYIVNEDVLPYGAALQA
Sbjct: 361 ILDMQPLMGAEDFGFYGEVIPGLFYFLGMKNETVGKFEQGHSPYYIVNEDVLPYGAALQA 420

Query: 483 SIATRYLLEHSPKSTSTKEHSHDEL 508
           SIATRYLLEHSPKSTSTKEHSHDEL
Sbjct: 421 SIATRYLLEHSPKSTSTKEHSHDEL 445

BLAST of CSPI01G27750 vs. TrEMBL
Match: M5VQJ2_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005751mg PE=4 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 1.1e-189
Identity = 329/440 (74.77%), Postives = 380/440 (86.36%), Query Frame = 1

Query: 68  FLNLILIFHLLISLLIPSSSSPIPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIHENPEL 127
           +++LI I  +++ LLI S  S  P+E++QIP  FL +A+  E+FD+MVG+RRKIHENPEL
Sbjct: 6   WVSLIFIVVVIMPLLISSDHSLSPEEVAQIPVKFLDHAKRQELFDFMVGVRRKIHENPEL 65

Query: 128 GFQEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQEGVEW 187
           GF+EFETSKLIRAELD++GI Y+YP+A TG+VGFIGTG PPFVAIRADMDAL +QE VEW
Sbjct: 66  GFEEFETSKLIRAELDQMGIPYKYPLAETGIVGFIGTGGPPFVAIRADMDALAMQESVEW 125

Query: 188 EHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAKKMLEA 247
           EHKSKVPGKMHACGHDAHVAMLLGAAKILQ++   +QGTVVLVFQPAEEGGGGAKKML+ 
Sbjct: 126 EHKSKVPGKMHACGHDAHVAMLLGAAKILQEHHHELQGTVVLVFQPAEEGGGGAKKMLDE 185

Query: 248 GVLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTIDPILA 307
           G L+ V AIFGLHV++ YP G  +++ GP  AASGFFEAVI GKGGHAA+PQHTIDPILA
Sbjct: 186 GALEHVGAIFGLHVAADYPIGSVVTRCGPFFAASGFFEAVISGKGGHAAIPQHTIDPILA 245

Query: 308 ASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLK 367
           ASN+IVSLQHLVSREADPL+SQVVT+ KFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLK
Sbjct: 246 ASNVIVSLQHLVSREADPLESQVVTVGKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLK 305

Query: 368 QRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNQNLYKHFRTVAVDMLGTKNILDMQ 427
           QRI EVIT QA VQRCNATV FNE  KPF+PV VN++NL++HFR VA D+LG +NIL+ Q
Sbjct: 306 QRIEEVITRQASVQRCNATVLFNERDKPFYPVAVNDKNLHEHFRNVAGDLLGPQNILERQ 365

Query: 428 PLMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQASIATR 487
           P+MGAEDF FY E IPG F+F+GMKNE+ GKFE GHSP++ VNEDVLPYGAAL AS+ATR
Sbjct: 366 PMMGAEDFSFYQEAIPGYFFFVGMKNESQGKFESGHSPFFRVNEDVLPYGAALHASLATR 425

Query: 488 YLLEHSPKSTSTKEHSHDEL 508
           YLLE+ PKST  K   HDEL
Sbjct: 426 YLLENQPKSTLPKGSFHDEL 445

BLAST of CSPI01G27750 vs. TrEMBL
Match: A0A067EXH6_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g013332mg PE=4 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 4.9e-185
Identity = 322/445 (72.36%), Postives = 371/445 (83.37%), Query Frame = 1

Query: 63  MPQRSFLNLILIFHLLISLLIPSSSSPIPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIH 122
           M     L+L++  +LL    I S  S  P+EL+QIP  FL +A+ PE+F WMV IRRKIH
Sbjct: 1   MGSSKLLSLLVTLYLLAPTSISSDVSLSPEELTQIPVKFLDFAKKPEIFYWMVNIRRKIH 60

Query: 123 ENPELGFQEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQ 182
           ENPELGFQEFETSKLIRAELD++GI Y++PVA+TGVVG+IGTG PPFVA+RADMDAL ++
Sbjct: 61  ENPELGFQEFETSKLIRAELDQMGIPYKFPVAVTGVVGYIGTGQPPFVALRADMDALAME 120

Query: 183 EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAK 242
           E VEWEHKSKVPGKMHACGHDAHVAMLLGAAK+LQ     I+GT+VLVFQPAEEGGGGAK
Sbjct: 121 ESVEWEHKSKVPGKMHACGHDAHVAMLLGAAKMLQVFRHEIKGTIVLVFQPAEEGGGGAK 180

Query: 243 KMLEAGVLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTI 302
           KML+AG L+ V+AIFGLHVSS +P G   S+PGP LAA GFFEAVI GKGGHAA+PQHTI
Sbjct: 181 KMLDAGALENVEAIFGLHVSSLFPVGTVASRPGPTLAAGGFFEAVINGKGGHAAIPQHTI 240

Query: 303 DPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKES 362
           DPI+AASN+IVSLQHLVSREADPLDSQV+T+AKF+GGGAFN+IPDSVTIGGTFRAFSKES
Sbjct: 241 DPIVAASNVIVSLQHLVSREADPLDSQVLTVAKFEGGGAFNIIPDSVTIGGTFRAFSKES 300

Query: 363 FIQLKQRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNQNLYKHFRTVAVDMLGTKN 422
            IQLKQRI EV+  QA VQRCNATV F  D K F+PVTVNN+NL++HF+ VA DMLG +N
Sbjct: 301 IIQLKQRIEEVVMKQASVQRCNATVTF--DDKSFYPVTVNNKNLHEHFQKVAADMLGVQN 360

Query: 423 ILDMQPLMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQA 482
           I + +PLMG EDF F+ E IPG FY+LGM +ET GKFE GHSPY+ VNED LPYGAAL A
Sbjct: 361 IKENRPLMGTEDFSFFAEAIPGYFYYLGMNDETKGKFETGHSPYFRVNEDALPYGAALHA 420

Query: 483 SIATRYLLEHSPKSTSTKEHSHDEL 508
           S+ATRYLLE+ PK+T      HDEL
Sbjct: 421 SLATRYLLENQPKTTLASRSLHDEL 443

BLAST of CSPI01G27750 vs. TrEMBL
Match: A0A061GTU8_THECC (Peptidase M20/M25/M40 family protein isoform 1 OS=Theobroma cacao GN=TCM_037723 PE=4 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 1.1e-184
Identity = 322/440 (73.18%), Postives = 378/440 (85.91%), Query Frame = 1

Query: 71  LILIFHLLISLLIPSSSSPI--PDELSQIPSFFLQYAQHPEVFDWMVGIRRKIHENPELG 130
           + LIF L  S+  P SS+P    + L+QIP  FL++A+ PE+ DWMVG+RRKIHENPELG
Sbjct: 7   VFLIFSLYSSMPTPISSNPSLNKEALAQIPINFLEFAKKPEILDWMVGLRRKIHENPELG 66

Query: 131 FQEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQEGVEWE 190
           F+E+ETSKLIRAELD++GI Y+YPVA+TGV+G+IGTG PPFVA+RADMDAL ++E VEWE
Sbjct: 67  FEEYETSKLIRAELDQMGIPYKYPVAVTGVIGYIGTGQPPFVALRADMDALAMEELVEWE 126

Query: 191 HKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAKKMLEAG 250
           HKSKVPGKMHACGHDAHVAMLLGAAK+LQ++   +QGT+VLVFQPAEEGGGGAKKML+AG
Sbjct: 127 HKSKVPGKMHACGHDAHVAMLLGAAKMLQEHQNDLQGTIVLVFQPAEEGGGGAKKMLDAG 186

Query: 251 VLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTIDPILAA 310
            L+ VDAIF LHVSS +P G+  S+PGPILAA+ FFEAVI GKGGHAA+PQHT+DPILAA
Sbjct: 187 ALENVDAIFALHVSSRFPVGVVASRPGPILAATSFFEAVINGKGGHAAIPQHTVDPILAA 246

Query: 311 SNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLKQ 370
           SN+IVSLQHL+SREADPLDSQVV+I KFQGGGAFNVIPDSVTIGGTFRAFSKESF+QLKQ
Sbjct: 247 SNVIVSLQHLISREADPLDSQVVSIGKFQGGGAFNVIPDSVTIGGTFRAFSKESFVQLKQ 306

Query: 371 RIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNQNLYKHFRTVAVDMLGTKNILDMQP 430
           RI EVI  QA VQRCNATV F  D+K  +PV++NN+ L+++FR VA DMLG +NI++MQP
Sbjct: 307 RIEEVIMKQASVQRCNATVIF--DEKSSYPVSLNNKELHEYFRKVAGDMLGLENIVEMQP 366

Query: 431 LMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQASIATRY 490
           +MG EDF F+ E IPGLF+FLGMKNET G+F  GHSPYY VNEDVLPYGA L AS+ATRY
Sbjct: 367 IMGGEDFAFFSEAIPGLFFFLGMKNETQGQFHSGHSPYYRVNEDVLPYGAVLHASLATRY 426

Query: 491 LLEHSPKSTSTKEHS-HDEL 508
           LLE+ PK TS  + S HDEL
Sbjct: 427 LLENLPKHTSPPKGSYHDEL 444

BLAST of CSPI01G27750 vs. TrEMBL
Match: A0A061GMB4_THECC (Peptidase M20/M25/M40 family protein isoform 5 OS=Theobroma cacao GN=TCM_037723 PE=4 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 1.2e-183
Identity = 323/441 (73.24%), Postives = 378/441 (85.71%), Query Frame = 1

Query: 71  LILIFHLLISLLIPSSSSPI--PDELSQIPSFFLQYAQHPEVFDWMVGIRRKIHENPELG 130
           + LIF L  S+  P SS+P    + L+QIP  FL++A+ PE+ DWMVG+RRKIHENPELG
Sbjct: 7   VFLIFSLYSSMPTPISSNPSLNKEALAQIPINFLEFAKKPEILDWMVGLRRKIHENPELG 66

Query: 131 FQEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQEGVEWE 190
           F+E+ETSKLIRAELD++GI Y+YPVA+TGV+G+IGTG PPFVA+RADMDAL ++E VEWE
Sbjct: 67  FEEYETSKLIRAELDQMGIPYKYPVAVTGVIGYIGTGQPPFVALRADMDALAMEELVEWE 126

Query: 191 HKSKVPGKMHACGHDAHVAMLLGAAKILQQNSR-LIQGTVVLVFQPAEEGGGGAKKMLEA 250
           HKSKVPGKMHACGHDAHVAMLLGAAK+LQ++   L QGT+VLVFQPAEEGGGGAKKML+A
Sbjct: 127 HKSKVPGKMHACGHDAHVAMLLGAAKMLQEHQNDLQQGTIVLVFQPAEEGGGGAKKMLDA 186

Query: 251 GVLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTIDPILA 310
           G L+ VDAIF LHVSS +P G+  S+PGPILAA+ FFEAVI GKGGHAA+PQHT+DPILA
Sbjct: 187 GALENVDAIFALHVSSRFPVGVVASRPGPILAATSFFEAVINGKGGHAAIPQHTVDPILA 246

Query: 311 ASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLK 370
           ASN+IVSLQHL+SREADPLDSQVV+I KFQGGGAFNVIPDSVTIGGTFRAFSKESF+QLK
Sbjct: 247 ASNVIVSLQHLISREADPLDSQVVSIGKFQGGGAFNVIPDSVTIGGTFRAFSKESFVQLK 306

Query: 371 QRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNQNLYKHFRTVAVDMLGTKNILDMQ 430
           QRI EVI  QA VQRCNATV F  D+K  +PV++NN+ L+++FR VA DMLG +NI++MQ
Sbjct: 307 QRIEEVIMKQASVQRCNATVIF--DEKSSYPVSLNNKELHEYFRKVAGDMLGLENIVEMQ 366

Query: 431 PLMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQASIATR 490
           P+MG EDF F+ E IPGLF+FLGMKNET G+F  GHSPYY VNEDVLPYGA L AS+ATR
Sbjct: 367 PIMGGEDFAFFSEAIPGLFFFLGMKNETQGQFHSGHSPYYRVNEDVLPYGAVLHASLATR 426

Query: 491 YLLEHSPKSTSTKEHS-HDEL 508
           YLLE+ PK TS  + S HDEL
Sbjct: 427 YLLENLPKHTSPPKGSYHDEL 445

BLAST of CSPI01G27750 vs. TAIR10
Match: AT1G51760.1 (AT1G51760.1 peptidase M20/M25/M40 family protein)

HSP 1 Score: 599.7 bits (1545), Expect = 1.6e-171
Identity = 297/440 (67.50%), Postives = 362/440 (82.27%), Query Frame = 1

Query: 68  FLNLILIFHLLISLLIPSSSSPIPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIHENPEL 127
           +++ +LI HLL   LI  SS+     LSQIPS FL  A+  + FDWMVGIRR+IHENPEL
Sbjct: 6   WVSFVLILHLLNPTLISCSSNG----LSQIPSKFLTLAKRNDFFDWMVGIRRRIHENPEL 65

Query: 128 GFQEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQEGVEW 187
           G++E ETSKL+RAEL+++G+SY+YPVA+TGVVG++GTG+ PFVA+RADMDAL +QE VEW
Sbjct: 66  GYEEVETSKLVRAELEKMGVSYKYPVAVTGVVGYVGTGHAPFVALRADMDALAMQEMVEW 125

Query: 188 EHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAKKMLEA 247
           EHKSKVPGKMHACGHDAH  MLLGAAK+L+++   +QGTVVLVFQPAEEGGGGAKK++EA
Sbjct: 126 EHKSKVPGKMHACGHDAHTTMLLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAKKIVEA 185

Query: 248 GVLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTIDPILA 307
           GVL+ V AIFGLHV++    G   S+ GP+LA SGFF+A I GKGGHAALPQHTIDPILA
Sbjct: 186 GVLENVSAIFGLHVTNQLALGQVSSREGPMLAGSGFFKAKISGKGGHAALPQHTIDPILA 245

Query: 308 ASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLK 367
           ASN+IVSLQHLVSREADPLDSQVVT+AKF+GGGAFNVIPDSVTIGGTFRAFS +SF+QLK
Sbjct: 246 ASNVIVSLQHLVSREADPLDSQVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKSFMQLK 305

Query: 368 QRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNQNLYKHFRTVAVDMLGTKNILDMQ 427
           +RI +VIT QA V  CNATV+F E++KPFFP TVN++ L++ F+ V+ DMLG +N ++MQ
Sbjct: 306 KRIEQVITRQASVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIENYVEMQ 365

Query: 428 PLMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQASIATR 487
           PLMG+EDF FY + IPG F F+GM+N+        HSPY+ VNE++LPYGA+L AS+ATR
Sbjct: 366 PLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHASMATR 425

Query: 488 YLLEHSPKSTSTKEHSHDEL 508
           YLLE    ST  K +  DEL
Sbjct: 426 YLLE-LKASTLNKSNKKDEL 440

BLAST of CSPI01G27750 vs. TAIR10
Match: AT1G51780.1 (AT1G51780.1 IAA-leucine resistant (ILR)-like gene 5)

HSP 1 Score: 556.6 bits (1433), Expect = 1.6e-158
Identity = 272/425 (64.00%), Postives = 341/425 (80.24%), Query Frame = 1

Query: 69  LNLILIFHLLISLLIPSSSSPIPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIHENPELG 128
           ++ +LI HLL S LI  SS+    +LSQIP  FL  A+  + FDWMVGIRR+IHENPELG
Sbjct: 7   VSFVLILHLLNSCLISCSSN----DLSQIPKNFLSLAKREDFFDWMVGIRRRIHENPELG 66

Query: 129 FQEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQEGVEWE 188
           ++E ETSKL++ ELD++G+SY+ PVA+TGV+G++GTG+ PFVA+RADMDALP+QE VEWE
Sbjct: 67  YEEVETSKLVKTELDKMGVSYKNPVAVTGVIGYVGTGHAPFVALRADMDALPIQEMVEWE 126

Query: 189 HKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAKKMLEAG 248
           HKSK+PGKMHACGHDAH  MLLGAAK+L+++   +QGTV+LVFQPAEEGG GAKK++EAG
Sbjct: 127 HKSKIPGKMHACGHDAHTTMLLGAAKLLKEHQEELQGTVILVFQPAEEGGAGAKKIVEAG 186

Query: 249 VLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTIDPILAA 308
           VL+ V AIFGLHVS+    G   S+ G ++A SG F+A I GKGGHAALPQ  IDP+LAA
Sbjct: 187 VLENVGAIFGLHVSNLLGLGQLSSREGLLMAGSGRFKATISGKGGHAALPQFAIDPVLAA 246

Query: 309 SNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLKQ 368
           SN+I+SLQHLVSREADPLDSQVVT+A F+G  AFNVIPDSVTIGGTFRA   +SF QLKQ
Sbjct: 247 SNVILSLQHLVSREADPLDSQVVTVATFEGSDAFNVIPDSVTIGGTFRALLPKSFEQLKQ 306

Query: 369 RIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNQNLYKHFRTVAVDMLGTKNILDMQP 428
           RIV+VIT QA V  CNATV+F ED+ P FP TVNN+ L+  ++ V+VDMLG +N ++  P
Sbjct: 307 RIVQVITTQASVNMCNATVDFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIENYVETLP 366

Query: 429 LMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQASIATRY 488
           +M +EDF FY + IPG F F+GM+N++       HSP++ VNE++LPYGA+L AS+ATRY
Sbjct: 367 VMVSEDFAFYQQAIPGHFSFVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLASLATRY 426

Query: 489 LLEHS 494
           LL+ S
Sbjct: 427 LLDSS 427

BLAST of CSPI01G27750 vs. TAIR10
Match: AT5G56660.1 (AT5G56660.1 IAA-leucine resistant (ILR)-like 2)

HSP 1 Score: 542.7 bits (1397), Expect = 2.4e-154
Identity = 271/433 (62.59%), Postives = 335/433 (77.37%), Query Frame = 1

Query: 71  LILIFHLLISLLIPSSSSP-IPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIHENPELGF 130
           L L F LL+ LL  SS SP I ++ SQI +  L++A+ PEVFDWMV IRRKIHENPELG+
Sbjct: 7   LSLTFQLLLFLLSVSSESPWIAEDTSQIQTKLLEFAKSPEVFDWMVKIRRKIHENPELGY 66

Query: 131 QEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQEGVEWEH 190
           +E ETSKLIR+EL+ +GI Y YPVAITGV+G+IGTG PPFVA+RADMDALP+QEGVEWEH
Sbjct: 67  EELETSKLIRSELELIGIKYRYPVAITGVIGYIGTGEPPFVALRADMDALPIQEGVEWEH 126

Query: 191 KSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAKKMLEAGV 250
           KSK+ GKMHACGHD HV MLLGAAKIL ++   +QGTVVL+FQPAEEG  GAKKM E G 
Sbjct: 127 KSKIAGKMHACGHDGHVTMLLGAAKILHEHRHHLQGTVVLIFQPAEEGLSGAKKMREEGA 186

Query: 251 LDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTIDPILAAS 310
           L  V+AIFG+H+S+  PFG A S+ G  LA +G FEAVI GKGGHAA+PQHTIDP++AAS
Sbjct: 187 LKNVEAIFGIHLSARIPFGKAASRAGSFLAGAGVFEAVITGKGGHAAIPQHTIDPVVAAS 246

Query: 311 NIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLKQR 370
           +I++SLQ LVSRE DPLDS+VVT++K  GG AFNVIPDS+TIGGT RAF+   F QL+QR
Sbjct: 247 SIVLSLQQLVSRETDPLDSKVVTVSKVNGGNAFNVIPDSITIGGTLRAFT--GFTQLQQR 306

Query: 371 IVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNQNLYKHFRTVAVDMLGTKNILDMQPL 430
           + EVIT QA V RCNA+V    + +   P TVNN++LYK F+ V  D+LG +  ++  P+
Sbjct: 307 VKEVITKQAAVHRCNASVNLTPNGREPMPPTVNNKDLYKQFKKVVRDLLGQEAFVEAAPV 366

Query: 431 MGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQASIATRYL 490
           MG+EDF ++ E IPG F  LGM++ET G +   HSP Y +NEDVLPYGAA+ AS+A +YL
Sbjct: 367 MGSEDFSYFAETIPGHFSLLGMQDETNG-YASSHSPLYRINEDVLPYGAAIHASMAVQYL 426

Query: 491 LEHSPKSTSTKEH 503
            E + K + +  H
Sbjct: 427 KEKASKGSVSGFH 436

BLAST of CSPI01G27750 vs. TAIR10
Match: AT5G56650.1 (AT5G56650.1 IAA-leucine resistant (ILR)-like 1)

HSP 1 Score: 530.8 bits (1366), Expect = 9.3e-151
Identity = 270/441 (61.22%), Postives = 334/441 (75.74%), Query Frame = 1

Query: 69  LNLILIFHLLISLLIPSSSSP--IPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIHENPE 128
           LN  L F LL+ LL  SS SP  +  ++S+IP  FL+ A+ PEVFD MV IRRKIHENPE
Sbjct: 3   LNNFLTFQLLLLLLRVSSESPWIVAGDVSRIPINFLELAKSPEVFDSMVRIRRKIHENPE 62

Query: 129 LGFQEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQEGVE 188
           LG++EFETSK IR+ELD +G+ Y +PVAITG++G+IGTG PPFVA+RADMDALP+QE VE
Sbjct: 63  LGYEEFETSKFIRSELDLIGVKYRFPVAITGIIGYIGTGEPPFVALRADMDALPIQEAVE 122

Query: 189 WEHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAKKMLE 248
           WEHKSK PGKMHACGHD HVAMLLGAAKILQQ+ + +QGTVVL+FQPAEEG  GAK M E
Sbjct: 123 WEHKSKNPGKMHACGHDGHVAMLLGAAKILQQHRQHLQGTVVLIFQPAEEGLSGAKMMRE 182

Query: 249 AGVLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTIDPIL 308
            G L  V+AIFG+H+S   PFG A S  G  +A +G FEAVI GKGGHAA+PQHTIDP++
Sbjct: 183 EGALKNVEAIFGIHLSPRTPFGKAASLAGSFMAGAGAFEAVITGKGGHAAIPQHTIDPVV 242

Query: 309 AASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQL 368
           AAS+I++SLQHLVSRE DP DS+VVT+ K  GG AFNVIPDS+TIGGT RAF+   F QL
Sbjct: 243 AASSIVLSLQHLVSRETDPSDSKVVTVTKVNGGNAFNVIPDSITIGGTLRAFT--GFTQL 302

Query: 369 KQRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNQNLYKHFRTVAVDMLGTKNILDM 428
           ++RI E+IT QA V RCNA+V    +     P TVNN +LYK F+ V  D+LG +  ++ 
Sbjct: 303 QERIKEIITKQAAVHRCNASVNLAPNGNQPMPPTVNNMDLYKKFKKVVRDLLGQEAFVEA 362

Query: 429 QPLMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQASIAT 488
            P MG+EDF ++ E IPG F  LGM++ET G +   HSP+Y +NEDVLPYGAA+ A++A 
Sbjct: 363 VPEMGSEDFSYFAETIPGHFSLLGMQDETQG-YASSHSPHYRINEDVLPYGAAIHATMAV 422

Query: 489 RYLLEHSPKSTSTKEHSHDEL 508
           +YL + + K + +    HDEL
Sbjct: 423 QYLKDKASKGSVS--GFHDEL 438

BLAST of CSPI01G27750 vs. TAIR10
Match: AT1G44350.1 (AT1G44350.1 IAA-leucine resistant (ILR)-like gene 6)

HSP 1 Score: 439.5 bits (1129), Expect = 2.8e-123
Identity = 213/388 (54.90%), Postives = 274/388 (70.62%), Query Frame = 1

Query: 102 LQYAQHPEVFDWMVGIRRKIHENPELGFQEFETSKLIRAELDRLGISYEYPVAITGVVGF 161
           L+    P+   W+  +RR IHENPEL F+E+ETS+LIR+ELDR+GI Y YP+A TG+  +
Sbjct: 77  LRLTYQPDNVAWLKRVRRTIHENPELAFEEYETSRLIRSELDRMGIMYRYPLAKTGIRAW 136

Query: 162 IGTGNPPFVAIRADMDALPLQEGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSR 221
           IG+G PPFVA+RADMDALP+QE VEWEH SKV GKMHACGHDAHV MLLGAA IL+    
Sbjct: 137 IGSGGPPFVAVRADMDALPIQEAVEWEHISKVAGKMHACGHDAHVTMLLGAAHILKAREH 196

Query: 222 LIQGTVVLVFQPAEEGGGGAKKMLEAGVLDKVDAIFGLHVSSSYPFGMAISKPGPILAAS 281
           L++GTVVL+FQPAEE G GAK M+E G LD V+AIF +HVS  +P G+  S+ GP+LA  
Sbjct: 197 LLKGTVVLLFQPAEEAGNGAKNMIEDGALDDVEAIFAVHVSHIHPTGVIGSRSGPLLAGC 256

Query: 282 GFFEAVIGGKGGHAALPQHTIDPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGA 341
           G F AVI  +    A      + +LAAS+ ++SLQ +VSREA PLDSQVV++  F GG +
Sbjct: 257 GIFRAVITSEDSRGA-----ANLLLAASSAVISLQGIVSREASPLDSQVVSVTSFDGGHS 316

Query: 342 FNVIPDSVTIGGTFRAFSKESFIQLKQRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTV 401
            +V PD+V +GGTFRAFS  SF  LK+RI EV+ +Q  V  C ATV F E Q   +P T 
Sbjct: 317 LDVAPDTVVLGGTFRAFSNSSFYYLKKRIQEVLMDQVGVFGCQATVNFFEKQNAIYPPTT 376

Query: 402 NNQNLYKHFRTVAVDMLGTKNILDMQPLMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQ 461
           NN   Y H + V +D+LG  +      +MGAEDF FY E+IP  FYF+G++NE +G    
Sbjct: 377 NNDATYNHLKKVTIDLLGDSHFTLAPQMMGAEDFAFYSEIIPAAFYFIGIRNEELGSVHI 436

Query: 462 GHSPYYIVNEDVLPYGAALQASIATRYL 490
            HSP+++++ED LP GAA+ A++A RYL
Sbjct: 437 AHSPHFMIDEDSLPVGAAVHAAVAERYL 459

BLAST of CSPI01G27750 vs. NCBI nr
Match: gi|449435806|ref|XP_004135685.1| (PREDICTED: IAA-amino acid hydrolase ILR1-like 4 [Cucumis sativus])

HSP 1 Score: 885.9 bits (2288), Expect = 3.2e-254
Identity = 441/445 (99.10%), Postives = 444/445 (99.78%), Query Frame = 1

Query: 63  MPQRSFLNLILIFHLLISLLIPSSSSPIPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIH 122
           MPQRSFLNLILIFH+LISLLIPSSSSPIPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIH
Sbjct: 1   MPQRSFLNLILIFHILISLLIPSSSSPIPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIH 60

Query: 123 ENPELGFQEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQ 182
           ENPELGFQEFETSKLIRAELDRLG+SYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQ
Sbjct: 61  ENPELGFQEFETSKLIRAELDRLGVSYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQ 120

Query: 183 EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAK 242
           EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAK
Sbjct: 121 EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAK 180

Query: 243 KMLEAGVLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTI 302
           KMLEAGVLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTI
Sbjct: 181 KMLEAGVLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTI 240

Query: 303 DPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKES 362
           DPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKES
Sbjct: 241 DPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300

Query: 363 FIQLKQRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNQNLYKHFRTVAVDMLGTKN 422
           FIQLKQRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNN NLYKHFRTVAVDMLGTKN
Sbjct: 301 FIQLKQRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNHNLYKHFRTVAVDMLGTKN 360

Query: 423 ILDMQPLMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQA 482
           ILDMQPLMGAEDFGFYGEVIPGLFYFLGMKNET+GKFEQGHSPYYIVNEDVLPYGAALQA
Sbjct: 361 ILDMQPLMGAEDFGFYGEVIPGLFYFLGMKNETVGKFEQGHSPYYIVNEDVLPYGAALQA 420

Query: 483 SIATRYLLEHSPKSTSTKEHSHDEL 508
           SIATRYLLEHSPKSTSTKEHSHDEL
Sbjct: 421 SIATRYLLEHSPKSTSTKEHSHDEL 445

BLAST of CSPI01G27750 vs. NCBI nr
Match: gi|659099850|ref|XP_008450808.1| (PREDICTED: IAA-amino acid hydrolase ILR1-like 4 [Cucumis melo])

HSP 1 Score: 844.3 bits (2180), Expect = 1.1e-241
Identity = 422/445 (94.83%), Postives = 434/445 (97.53%), Query Frame = 1

Query: 63  MPQRSFLNLILIFHLLISLLIPSSSSPIPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIH 122
           MP  S L+LILIFHLLISLLIPSSSS IP+ELSQIPSFFLQYA+HPEVFDWMV IRRKIH
Sbjct: 1   MPLCSSLSLILIFHLLISLLIPSSSSSIPEELSQIPSFFLQYAKHPEVFDWMVDIRRKIH 60

Query: 123 ENPELGFQEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQ 182
           ENPELGFQEFETSKLIRAELDRLGISYEYPVA+TGVVGFIGTGNPPFVAIRADMDAL LQ
Sbjct: 61  ENPELGFQEFETSKLIRAELDRLGISYEYPVAVTGVVGFIGTGNPPFVAIRADMDALALQ 120

Query: 183 EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAK 242
           EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAK
Sbjct: 121 EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAK 180

Query: 243 KMLEAGVLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTI 302
           K+LEAGVLD VDAIFGLH+SS YPFG+AISKPGPILAASGFFE+VIGGKGGHAALPQHTI
Sbjct: 181 KILEAGVLDNVDAIFGLHLSSRYPFGLAISKPGPILAASGFFESVIGGKGGHAALPQHTI 240

Query: 303 DPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKES 362
           DPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKES
Sbjct: 241 DPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300

Query: 363 FIQLKQRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNQNLYKHFRTVAVDMLGTKN 422
           FIQLKQRIVEVIT QAKVQRCNATVEFNEDQKPFFPVT+N+QNLYKHFRTVAVDMLGTKN
Sbjct: 301 FIQLKQRIVEVITKQAKVQRCNATVEFNEDQKPFFPVTLNDQNLYKHFRTVAVDMLGTKN 360

Query: 423 ILDMQPLMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQA 482
           ILDMQPLMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQA
Sbjct: 361 ILDMQPLMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQA 420

Query: 483 SIATRYLLEHSPKSTSTKEHSHDEL 508
           SIA RYL+E+SPKSTSTK HSHDEL
Sbjct: 421 SIAARYLIEYSPKSTSTKGHSHDEL 445

BLAST of CSPI01G27750 vs. NCBI nr
Match: gi|645274666|ref|XP_008242448.1| (PREDICTED: IAA-amino acid hydrolase ILR1-like 4 [Prunus mume])

HSP 1 Score: 671.8 bits (1732), Expect = 9.5e-190
Identity = 329/440 (74.77%), Postives = 380/440 (86.36%), Query Frame = 1

Query: 68  FLNLILIFHLLISLLIPSSSSPIPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIHENPEL 127
           +++LI I  +++ LLI S  S  P+E++QIP  FL +A+  E+FD+MVG+RRKIHENPEL
Sbjct: 6   WVSLIFIVLVIMPLLISSDHSLSPEEVAQIPVKFLDHAKRQELFDFMVGVRRKIHENPEL 65

Query: 128 GFQEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQEGVEW 187
           GF+EFETSKLIRAELD++GI Y+YP+A TG+VGFIGTG PPFVAIRADMDAL +QE VEW
Sbjct: 66  GFEEFETSKLIRAELDQMGIPYKYPLAETGIVGFIGTGGPPFVAIRADMDALAMQESVEW 125

Query: 188 EHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAKKMLEA 247
           EHKSKVPGKMHACGHDAHVAMLLGAAKILQ++   +QGTVVLVFQPAEEGGGGAKKML+ 
Sbjct: 126 EHKSKVPGKMHACGHDAHVAMLLGAAKILQEHHHELQGTVVLVFQPAEEGGGGAKKMLDE 185

Query: 248 GVLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTIDPILA 307
           G L+ V AIFGLHV++ YP G  +++ GP  AASGFFEAVI GKGGHAA+PQHTIDPILA
Sbjct: 186 GALEHVGAIFGLHVAADYPIGSVVTRSGPFFAASGFFEAVISGKGGHAAIPQHTIDPILA 245

Query: 308 ASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLK 367
           ASN+IVSLQHLVSREADPL+SQVVT+ KFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLK
Sbjct: 246 ASNVIVSLQHLVSREADPLESQVVTVGKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLK 305

Query: 368 QRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNQNLYKHFRTVAVDMLGTKNILDMQ 427
           QRI EVIT QA VQRCNATV FNE  KPF+PV VN++NL++HFR VA D+LG +NIL+ Q
Sbjct: 306 QRIEEVITRQASVQRCNATVLFNERDKPFYPVAVNDKNLHEHFRNVAGDLLGPQNILERQ 365

Query: 428 PLMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQASIATR 487
           P+MGAEDF FY E IPG F+F+GMKNE+ GKFE GHSP++ VNEDVLPYGAAL AS+ATR
Sbjct: 366 PMMGAEDFSFYQEAIPGYFFFVGMKNESQGKFESGHSPFFRVNEDVLPYGAALHASLATR 425

Query: 488 YLLEHSPKSTSTKEHSHDEL 508
           YLLE+ PKST  K   HDEL
Sbjct: 426 YLLENQPKSTLPKGSFHDEL 445

BLAST of CSPI01G27750 vs. NCBI nr
Match: gi|595803729|ref|XP_007202052.1| (hypothetical protein PRUPE_ppa005751mg [Prunus persica])

HSP 1 Score: 671.0 bits (1730), Expect = 1.6e-189
Identity = 329/440 (74.77%), Postives = 380/440 (86.36%), Query Frame = 1

Query: 68  FLNLILIFHLLISLLIPSSSSPIPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIHENPEL 127
           +++LI I  +++ LLI S  S  P+E++QIP  FL +A+  E+FD+MVG+RRKIHENPEL
Sbjct: 6   WVSLIFIVVVIMPLLISSDHSLSPEEVAQIPVKFLDHAKRQELFDFMVGVRRKIHENPEL 65

Query: 128 GFQEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQEGVEW 187
           GF+EFETSKLIRAELD++GI Y+YP+A TG+VGFIGTG PPFVAIRADMDAL +QE VEW
Sbjct: 66  GFEEFETSKLIRAELDQMGIPYKYPLAETGIVGFIGTGGPPFVAIRADMDALAMQESVEW 125

Query: 188 EHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAKKMLEA 247
           EHKSKVPGKMHACGHDAHVAMLLGAAKILQ++   +QGTVVLVFQPAEEGGGGAKKML+ 
Sbjct: 126 EHKSKVPGKMHACGHDAHVAMLLGAAKILQEHHHELQGTVVLVFQPAEEGGGGAKKMLDE 185

Query: 248 GVLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTIDPILA 307
           G L+ V AIFGLHV++ YP G  +++ GP  AASGFFEAVI GKGGHAA+PQHTIDPILA
Sbjct: 186 GALEHVGAIFGLHVAADYPIGSVVTRCGPFFAASGFFEAVISGKGGHAAIPQHTIDPILA 245

Query: 308 ASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLK 367
           ASN+IVSLQHLVSREADPL+SQVVT+ KFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLK
Sbjct: 246 ASNVIVSLQHLVSREADPLESQVVTVGKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLK 305

Query: 368 QRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNQNLYKHFRTVAVDMLGTKNILDMQ 427
           QRI EVIT QA VQRCNATV FNE  KPF+PV VN++NL++HFR VA D+LG +NIL+ Q
Sbjct: 306 QRIEEVITRQASVQRCNATVLFNERDKPFYPVAVNDKNLHEHFRNVAGDLLGPQNILERQ 365

Query: 428 PLMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQASIATR 487
           P+MGAEDF FY E IPG F+F+GMKNE+ GKFE GHSP++ VNEDVLPYGAAL AS+ATR
Sbjct: 366 PMMGAEDFSFYQEAIPGYFFFVGMKNESQGKFESGHSPFFRVNEDVLPYGAALHASLATR 425

Query: 488 YLLEHSPKSTSTKEHSHDEL 508
           YLLE+ PKST  K   HDEL
Sbjct: 426 YLLENQPKSTLPKGSFHDEL 445

BLAST of CSPI01G27750 vs. NCBI nr
Match: gi|720088118|ref|XP_010244354.1| (PREDICTED: IAA-amino acid hydrolase ILR1-like 1 [Nelumbo nucifera])

HSP 1 Score: 661.4 bits (1705), Expect = 1.3e-186
Identity = 323/446 (72.42%), Postives = 377/446 (84.53%), Query Frame = 1

Query: 62  SMPQRSFLNLILIFHLLISLLIPSSSSPIPDELSQIPSFFLQYAQHPEVFDWMVGIRRKI 121
           +M   +++ LI I  L + + I S SS      ++I + FL  A+ PE+ DWM+GIRRKI
Sbjct: 7   NMEFSTWVCLIFILQLFLPMPISSDSSLSTKGFAEISANFLNLAKKPELADWMIGIRRKI 66

Query: 122 HENPELGFQEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALPL 181
           HENPELG+QEFETSKLIRAELD++GI Y++PVA+TGVVGFIGTG PPFVAIRADMDALP+
Sbjct: 67  HENPELGYQEFETSKLIRAELDKMGIPYKHPVAVTGVVGFIGTGGPPFVAIRADMDALPM 126

Query: 182 QEGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGA 241
           QE VEWEHKSK+PGKMHACGHDAHVA+LLGA KILQ++   +QGTVVLVFQPAEEGGGGA
Sbjct: 127 QENVEWEHKSKIPGKMHACGHDAHVAILLGATKILQEHQNNLQGTVVLVFQPAEEGGGGA 186

Query: 242 KKMLEAGVLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHT 301
           KKML+AG+L+ V+AIFGLHVS   P G   S+PGPI+A SGFFEAVI GKGGHAA+PQHT
Sbjct: 187 KKMLDAGILENVEAIFGLHVSVDLPIGKVASRPGPIMAGSGFFEAVISGKGGHAAIPQHT 246

Query: 302 IDPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKE 361
           IDPILAASN+IVSLQHLVSREADPLDSQVVT+AKFQGGGAFNVIPDSVTIGGTFRAFSKE
Sbjct: 247 IDPILAASNVIVSLQHLVSREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKE 306

Query: 362 SFIQLKQRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNQNLYKHFRTVAVDMLGTK 421
           SF+QLKQRI EVIT QA VQRC+ATV+F  DQKP FPVTVN+Q+L+++F+ VA DMLG +
Sbjct: 307 SFMQLKQRIEEVITGQAAVQRCSATVDFFLDQKPHFPVTVNSQDLHEYFQQVAGDMLGIR 366

Query: 422 NILDMQPLMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQ 481
           N+ DMQPLMG+EDF F+ E +PG ++FLGMKN+T GK E GHSPY+ +NED LPYGAALQ
Sbjct: 367 NVKDMQPLMGSEDFAFFSEAVPGYYFFLGMKNDTRGKLEPGHSPYFTINEDALPYGAALQ 426

Query: 482 ASIATRYLLEHSPKSTSTKEHSHDEL 508
           AS+A RYLLE  PKS   +   HDEL
Sbjct: 427 ASLAARYLLEFHPKSHFQETKFHDEL 452

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ILL4_ARATH2.9e-17067.50IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsis thaliana GN=ILL4 PE=1 SV=2[more]
ILL1_ORYSI1.3e-16568.86IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica GN=ILL1 PE=2 ... [more]
ILL1_ORYSJ4.8e-16568.61IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica GN=ILL1 PE=... [more]
ILL5_ARATH2.8e-15764.00IAA-amino acid hydrolase ILR1-like 5 OS=Arabidopsis thaliana GN=ILL5 PE=3 SV=1[more]
ILL2_ARATH4.2e-15362.59IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsis thaliana GN=ILL2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0LYS1_CUCSA2.2e-25499.10Uncharacterized protein OS=Cucumis sativus GN=Csa_1G575150 PE=4 SV=1[more]
M5VQJ2_PRUPE1.1e-18974.77Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005751mg PE=4 SV=1[more]
A0A067EXH6_CITSI4.9e-18572.36Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g013332mg PE=4 SV=1[more]
A0A061GTU8_THECC1.1e-18473.18Peptidase M20/M25/M40 family protein isoform 1 OS=Theobroma cacao GN=TCM_037723 ... [more]
A0A061GMB4_THECC1.2e-18373.24Peptidase M20/M25/M40 family protein isoform 5 OS=Theobroma cacao GN=TCM_037723 ... [more]
Match NameE-valueIdentityDescription
AT1G51760.11.6e-17167.50 peptidase M20/M25/M40 family protein[more]
AT1G51780.11.6e-15864.00 IAA-leucine resistant (ILR)-like gene 5[more]
AT5G56660.12.4e-15462.59 IAA-leucine resistant (ILR)-like 2[more]
AT5G56650.19.3e-15161.22 IAA-leucine resistant (ILR)-like 1[more]
AT1G44350.12.8e-12354.90 IAA-leucine resistant (ILR)-like gene 6[more]
Match NameE-valueIdentityDescription
gi|449435806|ref|XP_004135685.1|3.2e-25499.10PREDICTED: IAA-amino acid hydrolase ILR1-like 4 [Cucumis sativus][more]
gi|659099850|ref|XP_008450808.1|1.1e-24194.83PREDICTED: IAA-amino acid hydrolase ILR1-like 4 [Cucumis melo][more]
gi|645274666|ref|XP_008242448.1|9.5e-19074.77PREDICTED: IAA-amino acid hydrolase ILR1-like 4 [Prunus mume][more]
gi|595803729|ref|XP_007202052.1|1.6e-18974.77hypothetical protein PRUPE_ppa005751mg [Prunus persica][more]
gi|720088118|ref|XP_010244354.1|1.3e-18672.42PREDICTED: IAA-amino acid hydrolase ILR1-like 1 [Nelumbo nucifera][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002933Peptidase_M20
IPR011650Peptidase_M20_dimer
IPR017439Amidohydrolase
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0016787hydrolase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
biological_process GO:0006558 L-phenylalanine metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0047980 hippurate hydrolase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G27750.1CSPI01G27750.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 171..484
score: 7.0
IPR011650Peptidase M20, dimerisation domainGENE3DG3DSA:3.30.70.360coord: 279..397
score: 3.0
IPR011650Peptidase M20, dimerisation domainPFAMPF07687M20_dimercoord: 285..379
score: 5.9
IPR011650Peptidase M20, dimerisation domainunknownSSF55031Bacterial exopeptidase dimerisation domaincoord: 279..398
score: 1.01
IPR017439AmidohydrolaseTIGRFAMsTIGR01891TIGR01891coord: 114..476
score: 2.7E
NoneNo IPR availableGENE3DG3DSA:3.40.630.10coord: 398..493
score: 8.2E-105coord: 109..276
score: 8.2E
NoneNo IPR availablePANTHERPTHR11014PEPTIDASE M20 FAMILY MEMBERcoord: 44..493
score: 8.0E
NoneNo IPR availablePANTHERPTHR11014:SF55IAA-AMINO ACID HYDROLASE ILR1-LIKE 1-RELATEDcoord: 44..493
score: 8.0E
NoneNo IPR availableunknownSSF53187Zn-dependent exopeptidasescoord: 107..295
score: 9.58E-88coord: 400..491
score: 9.58

The following gene(s) are paralogous to this gene:

None