CSPI01G25270 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI01G25270
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Toprim domain protein
Location	Chr1 : 20731464 .. 20740158 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GAATTTTATTTAGTAAAGAAAAAAAGGAGAAAAATCCTGCTAGTTGTGCAGGAAAGCCTCCCCCTTCCGTGATCGGGTTCTTTTGTCGTCGGAAGCAAGCCGCCGAAGAAAGTTGGGGTGGTTTCCAGCAACCAAAACCCACTCTTCGCACACCCCTAACTCCTTCCTCTCTTTACCAATATTCATTTCAATGCCCCTGCCGGTTTCTTCAGTCTCTACCTACCTACTATGTTATTTTTAACTCTGTAGTGTAGACTTGAAATGGTGGCCATACTCAGCTGATCACCTTAACCCTACCAATAGCCAGAATGCGTTTTCTTCATCACAATCACCGTCTCTATAACCCCTTTTCAAAACTCTCTTCATTTTCCTCACCATTTCGTCTAATGGGTTCCTTTCCTTTATGCAAATCAACCTCTCTGGTTTTCCTGTCACACCTTTCTTCTTCTTTTTCGCAGAAGTATTTCCTGTACAGAAGCATTTCTCTTCTCCATGGTTCATTTCCTGTACGACCCATGTCCCTTGTGAAACCCTTTGCCATGAAACCAAATGGGGTTTCTTCTTTTACTTCTCATGCCAATGTTCCCAGACCTCCAGGTGCTATTATTGGAACTTTTTATGGTTGTTTAATTGTAGTTGGATTTATGATTATTTCTGCTTAATACTCATGTTGTGGGGTAACTTGATGTTTCTTGGCTGGGTGGTTACTCTTTTTTTTTCCCCTCTTTTTGGATTGGGATGAAATAGGATTTTTGAAGGCAGAAAAGTAGTTTGCAGGCAATGTATTTTGAGGATGGGACTTGGGAGAGGACTTGACGTTTGTTTAGTTTTAATGTTATTCGCTTTCTTCTTTGTGCAGCCCTTTTGGGGAATCCACCAGACAAGGCTTCAATCTCCACTCGATTGAATATTCTAAGAAAAAAATTGCAGGATCTTGACATTGATATCGAAGCTTGTGTTCCTGGGCAGTTTTCCAGTTTGCTTTGTCCAATGGTATTTTGTTTCTAATATCAGTTTCTTTATGCACACTGTGTTCTGTTTTGAGATATTCATCCAATTTGTAGAAGTGAAATGAAAACAGCTGCCAACTTCTATATCTTATTTTTTGTACAACGTAAACATTTAGAGGCCAATTTTAGAATTACTGTATGAACTTATAACTTCCAAGTGCAAAGAGGTTGGTATTATCATTATTATATTTATTTTAAAATTTTAATTTTTTTAACTTTACTCGATTAATTATGTATCAAATTGGTGCCAGTGTAAAGGTGGTGATACAAAGGAACGATCCTTCTCCCTAAATATCTCAGAAGATGGGTAAAGATTTTATAGCTTCTCTCTCGTCGAGTTTTTCTTAGAATCTTTTTATTTGATGTACTCATAGGCTTTGTGTGTTTGTGTTTTTCTTATATGGTTTGTTTTAAATGTTTTTGTGGAAGTTATTTAGGGTGACTTGTAATTATTAAGCTTCTTCCTCAGTTGAGTTTAAGTCATTTTACTTTTTATACTATTAGTATTTGTTATTTTTTTTGAAGAGGAAAGAAGTCTCAATATTATTAATGAATTAAATAGAGACAAATGCTCATAGTACTAGAGGGTATGGGCAAAGAAAGAGAGAGAGGCAGATCAACAAACGCACCCGGACATCTCATTTAGGTTGACACTCCTTAGCGCCCTCATCATGTCCATAGTGTGGAAAGAAATAAAAATGATCTTTTCTCTAGGTTGATAGTAGTCAATACAAAGCCAAAATACAAGCCCAAAATAAAGTAGTCAGTCCATACAGAACATTAGAAAAAAAGCATCAACGATATATTAGAAAAACATTCCTCGTCTCTGCATAGATCCATCTCTTGCCAGATAAGTGAGAATTTGTTAAAAAAGGATGTAACATCCATGGTCCCTTGCCTGCATTCATGAACTAGTTTCTGTACAGTATATTGATGAGACATTTTGATGTTTGGAATACAGTTTTTGGGCTGTGTCCTAAGTATGTTTAGGGTAAAGGTTTGCCAATCTGTGGCTCCATGCTATTAATTAATGTGGATCAAAGAAGAGAATCTTCATGCCCTTCTCCTTTACCTTCCTTGGCTTTTATCTCTTTCTTTGAACTAACTTTGGCCCACCGTGGTCCCCTATCTCCTTCCTCACTGTCTAAGTTTCCCTTCACACGTGCTTCCTCTTTCTCTTACGCATTCTACTGTATTGTAGAATAATAGGATGTCTACCATATTGTATCATAAAACAACATCAAAAAAGGAGAAGAAAAACAAAACAAAAAGACAAACTATACCATATAATATACAATCAATATCAAATCAAGTAATAATTTTAAGTCATTTAAAATTGGCAACTCAACAACCTCACGCAACTTTTCTTTTAGTCAAATAATGGTCAATTTAAAATAGGGGTCATTTATGACTATTTTCCAAAACTCAAGGACTAAGTGTTTACTTGGGAGTTAAATAGACATCAACACTAATCCTCAAGGACCAAGTATATGTTTCCCTACGTTTTGTAACAATCTTTTTGGTTATCAATTTAATTTAATTATAAATATGATTATTTTCAAATTCTTTATTTATTTTTTCTTGATATACATATATGGCTGTGACAAGTTGATGGACTTAAACTCCATAGTTCGTTCACTACTAACCTTAGTTTGATTCTTAGCTTTTTTTTTTTTTTTGTTCAATGAAATTAATACTTTTTTTCTAATGAAAAGTGGTTGGATTTCCTTCCCATCCACTGGACTTTTCAATTTTTCAATTGCATTGACCTGAATGTATTCTACGGCCTTTTTCTTTTTGAGTATCACATGTTTCTAATTTCCATTTCAAATGACCAGGGAGGCTGCTGTTTGGAATTGTTTTCGTGGAAAATGTGGTTGGAAAGGCCATACTCTGGTATTTATAATAATTCAATTAGATATGCTTTCACACAAAATGTGGTTTGGGCCCTTTCACGTAGGGTTGATTTGAATAGTTCTTCAGTTATGTTTTGCTTTGTTTTTTTCTTTCTTTTTTATATATATATATTTTTATGAGATAATTTGGTTTTTGTTTCAATTTCTTGTATTTTGTTCAAATATCATAGAATGTTCCATAAAGTTTAATTGAGTGGTATCCTTACTACTTGTGGGAAGGGGTTGACGATGAAGGGTCTCATTTGGTTGATTGGAAATTAGTGGTTAAGCTAGTGGAACTAGTAGGATTAAGGATTCTTCATGGATTGCTTTGTGGTATAAAGTTATTGTGAGCAGATATGCCTCACACCCATTGATTTTTAAAGCTCAAGGCACAATAGTCTTCTGGGCACACGCACAAGGCGCAAAAGAAAAAGCTTAAGCTTTTTTGTTTAGGTGTACAGTACTACATTAAGAAGAGAGCCTAGACAAAACCCTTAGTTATTACTAGTTTTTTTTTTTAAAAAATAATGAAGACCCTCCAAACGCAGGGTTTTGAGCCTTTGGGCATCACAAAAGACGCATGAATGAGACGCTTCTTGTTTTGTGAGTCTCGCCTTTTCCAGCAAGGCACCAAACTTGGGCTTTGAGCCTAGGTGCGCACCCGAGTGGGCTTTTCAAAAACCGTCCAGAAAGCAGTTGTTCTGTCTAAATACATAAGAAAAATGAAAAATCTATTGGAAGCTATCTGATAGATACCTACATTTAGATAGGGGTAAGGGTAGTTAGTTATATAAGGCAGTTAGATGGTTATAAATAGGGAGGTGGGGAGGGAAGAAGGGCATGAAGAATTTGGTGAAGAATTAGGGCTGCAACATTCCTTGAAAGAGAGGAAGGGCAGAGGGTTAGGGTTCTTTTACTTTTCTTTTGCTGCTTTTATATTGTAATTTTCTGTTTGAGATATCAATAATTTTTTTTTTGAAATGGAGACGAGCTTCTTTATTAATAAAGGAAAATGAGACTAAAGCTCAAAGTACAAGAGAATTATACAAAGAGCAAAAAGTAAAGACAAACAACCAAACAAACTGCCCAAAGACAACCTCAGCGAATACAAGGAAAAAACGACTAAAACAAAACAAATGCAAAGAACAAGCTAAAAACGCGAAAACCAACAGAAAAAATGAAGATAAAACAGAACCAAAACGAAGAGAAATAAATACAAAGCATAACAAAATAACTTTCTCAAAAGGAAACCATTTGGATCTAGAGACTAGCAAAAAGAAGCAACCGGTGAACTCAAAGCTAACTTGCCAAAGGAGACCAAAACCAGAAGAGGAACAAAAGAATCTTTCATAATATCAGCCTCAGCAAAAGGTTGTACATCCTATACAAAGCTTTAACACACCATGTTGAATCTTCAAATTGATGTTCCAATTGAAAACTCATCCCCCATGCATGAAGAAGTACTAAAGATATATACCAAAATATTTCTCCCACAAGCCACTTTAATAGATTCCAGCACCTTTTTCAACCATAACTTGATTCATGACATATCGATAAAATAGAAACACTATATCGGTGTTCTATCACTATCACATATGGATTCCCTTTATTCTCTCAATTTCTCAAATGGTTTGTTGGGCAAGGCTTGAAAAGTTTATTTTGGGAGATCGTTGGACAGGTGGGTATAAAACTTATATTATTAAAATAATAGAAAACCAGTTGGCGTGTCTATTATTTTATTTATACTTTTTTACTTTCCAACATGTTAGGACACTTCCAAGACACATCTAACTATTGTCTTTCAATATCTATCATTGGTAGGTTCTAAACTTTTTCTATATTTGTAAATATTTTAAGCAGTTTTGTCATTTATAGAATCCTTAATAACAACGACAACAATGTGTCAATGTACTGTGCCCTACTTTGCTAGGTTCTAGACCAACATATTCTTTGGGAGAGAAGGGACTCTAGAATTTGGTCTCCTGATCCTTTGCTAGGCTTCTCCTCTTGGTTCCTATTACATATTTTGTTTGTTGCTTTTCCGACCCAGATTAATTCTGCTTTTGTCGGAATTTGGAAGAGTAAAATTCCAAAGAAGTTCAGTTTTTTTCCTGTGACAGGGTTTACATGGTGATTATCATTTAACAACATAATTTTTATGTATTGAGAAAAGGACTATATCATCGGCCAGTTGTACGTGGTTTATGCTACGTGATTATTTACAGGTTCAAAACTGAGATTGATGGCAACAACTTGAGTCGAGCTACTAAGCTAAGGCAACCCACTAGGCCACTACCAAGTTTAAAAGGGAGGGAGATTTGGGATCTCCAGGCCTAAAACCTCTTGATCCAATGATCTTCTCTTCTTTTCTTATTATTATTTAGGCTAATTGAATGGTGGGGTTTTATTTATTTATTATTGTTTTCTTTATTTTTCTTTTCTTTGGGTAGGCCAACTGGCTTTTAATGATGGTGTCCTATCACTCGTGTATTTGTTTTCGATTGCTGTGAGCGTTTCTTATAAAAATGGAAAAAAAGTTTACAATCCAAATTGGTGCCTTTTTTTTCTTATTTCTTCCGTTACTTTACTTTACTGTTTTTATTGGTTTTATTTTTATTTCCATTGAGCATTAGTCTCTTTTTAATATATGAATTTTTTGTTTATTTATTTATGTTGTTCATTTTGAAAAACAAGAACTTATTGCTTCAATCTATATGAATGCGTGATTGAGTTGTTTGTCTTAGCATAGTTTGTGATGGCAGGCCTTTACTGATGGTCGGTCATCATATAAAGATTTAGGACAAGTTGCACTTAAGCAGAACATACGGAAAATTACAGTGGAGAGTCTACAACTTGAACCACTGTGCGATGAGGTTCTTGATTTTATATTTTTCTTCATTTTGATGATAATTATTGCTTTCCGGTCTATGTGAAAGCATATATGTATATTATTGTGTAGTTTTCTAAATATGTTATTTATAGGTGGTCATCAATTCTTTTTCCTTTATTGTCATCCTACATTTTGTTCTATTAGGAATACTTATTTTTCTTGAAATTAAGAATTTAAAATTATCCCAAAGTGCATTAAAACCTGTTAAAACACATTAAAAATAGGTATACTTTCATCTTTATTTTTAGGAATTTTTTTAACAACTTTATAATTTTTTTTCCCTATATGTTTGGAGTGCTAAGTTGTTTAACTAAGAATCGACTATTGAATCGTATAAGTTGATTTCTTTAAACAAATGAAATTTATTTTAAAATTTTTACTATAATTTTGTAAACTAAGCATGGATATAAATATTTCCTTTTCATATGCAATTCAATTGCTCTTATAATTGTGGAACATAGAGAAAATTATTATGTTTCTTGTTTCAGCTGGTTGATTATTTCGCGGAGCGATTGATATCCAAGCAAACATTGTTAAGAAATTCAGTTATGCAGAAAAGATCCAATAATCAGGTAAAAGGAGTTATGTTGAATTGAAGTAGTCATGGAGCATTGGCCTTCGGATGATAATCTCTTAGGGTTCGTTTGGTTTAAGATTTTTCTACCTAAGAATTAGCACATACATTTAAAACTCATGTTTGTTTCACAATTTTCTAACTTTTGCTGGGAATAACTGATTCCCAGGCATCCTTATTTACCACATACCAGGGTTTTTTATTCTCTTCCTAAACAGTGAGTTTCCTCTTTTTCCCCTCTATTTTTTTATTATTTTATTTGAAATACAGTTAACTAATTAATACTAACTTTAAAAAAATAATATAAAATATTTATTTTAATGAATTAGTAACAGTCTAATAAAATATTTACCTAACTTATTTTATAATAAAAAATTATTAAATGAATACAATTATTTCTTTACAAAAATTATTATTTAATTAGCTAACAATATGTTAAATATATTTTAATTGATAAATTAAGATCGATTAAATTTAGTAAGACAAAAATTTATTTTTCAATAATAATAATTGATTACTTTATTGTTTTTTCAATAATCAAGCCATTATTTCTTAAGATTAGTTTTAATTTATATTACTTCATTCATGAAAAACATATAATATTTATAAAACCTTTTTTAGTATTCTTTATTGATATATCATACTCCTATCTCATTTTTTCATTTTTCATTTAATATATTGAATTAAAATATATTTTCTTATAGTATTTAATTGAAATTGAAATTACTAACGATAGAGTTAATTAATTATAAACATGATTAACTATTCGATTGTAATTAATATTATCTTCGTTAAATATTTCAATGGGTTAATTATAATTATCAATTTCTTTAAAAAAATTAATTATAAATCTCAATTTTAAAGTATTTATAGTAAAAAATATTATTGATTCCAAATTAATTAATTATTATCAATATATTTTTTAATTAATTAATCTATTAAGTTTATATAAGAATTTTCTGATTAATTTCATAAATTAAATTCAAACATATTTTTTGTCCTTAAAATTCTAATAGCATTTTCTATGCTCAACCAAACACTGTCATCTTATTCCCAGGTATTCCATTCCTAGGCATCTATGAAACCTTAGACCAAAGGACTCCTTAATGGCATCTAAGCATGCTAAATGTATTGATAAAGTGTGCTTCATTAACTGTGGAAGAATGCTTCAATTTCATAAGATGCAGTTTGATCTTTTGTAAAATTTTGTTGTCTTATAGAGCTGCCTTGACTTATTATTATTTATTTATTAAAAGGAAACAAGACTCTTCATTGAGTGAAAATAAGTATTCTTAATATTCTGCCATTGAATTAGATAAGAAATATCGGGAATGTATTGCATTTTGAATCTTTGGAAAGGAAAACTAAAAATTAAAAAAGAAAAAAAAGGAAACCTAGGAAACAAGTTTACCTTTGACTTCTCTCTCTCTTTTGTGGATTAAGGGGGACGGTTTCTGTAAAGTTACAAGTTTTGACCTTTTTAATGATTCGTTGTGATGGAGCTTCATCATCTTAGTTGCTTTTGTCTTCTGCTTATTTTGTGCATTTGGGGGTGGAGAGGGGCAGATATCAAAGGGAAATTATGTAGTTTTAATTACAAAGCATATTTTGACAGCAAGAATGTTGAAACTTGAAAGCTGTGATTATTTTGGTGATATTGGAGCCATAACTCTTGCCTTGTATAAATTTTAACATGCTTACTGGAGGAAAATTTTAGCATGATTTGCTGCTTCTAATTGCTTACTTGTTTCTACAGATTGCTGTTGCATTTACATATTATCGAGGTGGAGCATTAATTAGTTGCAAGTATCGTGATGCCAAGAAAAGGTTCTGGCAGGTTCTTGGTTCAACTTATAAATAAAACTGTATGATAAATTTTTACTTATCCTGATTCTAAAGGCTTAAAATATATGCTTAGAGTAAATTCAGTTCATCTTAAATCATTTTGATTTTACCTTCTTCCCACTAGAAAAGTGTGCCGTCTGCTTTACTTGTATAATGTTATTGTAAGAAAATATATATAATCATCATCTGATTGTCTACCTTCCTTTATTTCAGGAGGCCAAAACTGAGAAAATATTTTATGGATTGGATGACATAGATGGTGCAAGTGATATCATCATAGTATGAGTTATCTAGTATGGAAAATCACTCCACTTAGATTTATTACTCATAATTTTCCTTTGTAATATAGGTTGAAGGGGAGATAGACAAGCTCTCAATGGCAGAAGCTGGTATCCATAATTGCGTGAGTGTTCCTGATGGTGCACCACCATCAGTTTCCAAAAAAAACGTACCTCCTGCACATCAGGTTACACTTTGA

mRNA sequence

ATGCGTTTTCTTCATCACAATCACCGTCTCTATAACCCCTTTTCAAAACTCTCTTCATTTTCCTCACCATTTCGTCTAATGGGTTCCTTTCCTTTATGCAAATCAACCTCTCTGGTTTTCCTGTCACACCTTTCTTCTTCTTTTTCGCAGAAGTATTTCCTGTACAGAAGCATTTCTCTTCTCCATGGTTCATTTCCTGTACGACCCATGTCCCTTGTGAAACCCTTTGCCATGAAACCAAATGGGGTTTCTTCTTTTACTTCTCATGCCAATGTTCCCAGACCTCCAGCCCTTTTGGGGAATCCACCAGACAAGGCTTCAATCTCCACTCGATTGAATATTCTAAGAAAAAAATTGCAGGATCTTGACATTGATATCGAAGCTTGTGTTCCTGGGCAGTTTTCCAGTTTGCTTTGTCCAATGTGTAAAGGTGGTGATACAAAGGAACGATCCTTCTCCCTAAATATCTCAGAAGATGGGGAGGCTGCTGTTTGGAATTGTTTTCGTGGAAAATGTGGTTGGAAAGGCCATACTCTGGCCTTTACTGATGGTCGGTCATCATATAAAGATTTAGGACAAGTTGCACTTAAGCAGAACATACGGAAAATTACAGTGGAGAGTCTACAACTTGAACCACTGTGCGATGAGCTGGTTGATTATTTCGCGGAGCGATTGATATCCAAGCAAACATTGTTAAGAAATTCAGTTATGCAGAAAAGATCCAATAATCAGATTGCTGTTGCATTTACATATTATCGAGGTGGAGCATTAATTAGTTGCAAGTATCGTGATGCCAAGAAAAGGTTCTGGCAGGAGGCCAAAACTGAGAAAATATTTTATGGATTGGATGACATAGATGGTGCAAGTGATATCATCATAGTTGAAGGGGAGATAGACAAGCTCTCAATGGCAGAAGCTGGTATCCATAATTGCGTGAGTGTTCCTGATGGTGCACCACCATCAGTTTCCAAAAAAAACGTACCTCCTGCACATCAGGTTACACTTTGA

Coding sequence (CDS)

ATGCGTTTTCTTCATCACAATCACCGTCTCTATAACCCCTTTTCAAAACTCTCTTCATTTTCCTCACCATTTCGTCTAATGGGTTCCTTTCCTTTATGCAAATCAACCTCTCTGGTTTTCCTGTCACACCTTTCTTCTTCTTTTTCGCAGAAGTATTTCCTGTACAGAAGCATTTCTCTTCTCCATGGTTCATTTCCTGTACGACCCATGTCCCTTGTGAAACCCTTTGCCATGAAACCAAATGGGGTTTCTTCTTTTACTTCTCATGCCAATGTTCCCAGACCTCCAGCCCTTTTGGGGAATCCACCAGACAAGGCTTCAATCTCCACTCGATTGAATATTCTAAGAAAAAAATTGCAGGATCTTGACATTGATATCGAAGCTTGTGTTCCTGGGCAGTTTTCCAGTTTGCTTTGTCCAATGTGTAAAGGTGGTGATACAAAGGAACGATCCTTCTCCCTAAATATCTCAGAAGATGGGGAGGCTGCTGTTTGGAATTGTTTTCGTGGAAAATGTGGTTGGAAAGGCCATACTCTGGCCTTTACTGATGGTCGGTCATCATATAAAGATTTAGGACAAGTTGCACTTAAGCAGAACATACGGAAAATTACAGTGGAGAGTCTACAACTTGAACCACTGTGCGATGAGCTGGTTGATTATTTCGCGGAGCGATTGATATCCAAGCAAACATTGTTAAGAAATTCAGTTATGCAGAAAAGATCCAATAATCAGATTGCTGTTGCATTTACATATTATCGAGGTGGAGCATTAATTAGTTGCAAGTATCGTGATGCCAAGAAAAGGTTCTGGCAGGAGGCCAAAACTGAGAAAATATTTTATGGATTGGATGACATAGATGGTGCAAGTGATATCATCATAGTTGAAGGGGAGATAGACAAGCTCTCAATGGCAGAAGCTGGTATCCATAATTGCGTGAGTGTTCCTGATGGTGCACCACCATCAGTTTCCAAAAAAAACGTACCTCCTGCACATCAGGTTACACTTTGA

Homology

[Close]

BLAST of CSPI01G25270 vs. Swiss-Prot
Match: TWIH_ARATH (Twinkle homolog protein, chloroplastic/mitochondrial OS=Arabidopsis thaliana GN=At1g30680 PE=1 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.7e-70
Identity = 162/332 (48.80%), Postives = 208/332 (62.65%), Query Frame = 1


Query: 1   MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISL 60
           MRFL    +++  F KLS   S   LMGS    +   L   +   SS S  Y   R +S 
Sbjct: 1   MRFLLRLPQIH--FRKLSCSMSV--LMGSKQFLEFCLLPSFASYPSSPS--YSSSRQVSS 60

Query: 61  LHGSF-PV---RPMSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILR 120
           +   F PV   RP+S   P+  + NG+SS+ S   VP P        DK  + +RL  LR
Sbjct: 61  VSRRFRPVLASRPVSKNSPYYQRTNGLSSYNSIPRVPTPVDTEVEA-DKRVVLSRLVTLR 120

Query: 121 KKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKG 180
           +KL +  +D E C PGQ S L+CP C+GG++ E+S SL I+ DG +A WNCFRGKCG KG
Sbjct: 121 RKLAEQGVDAENCPPGQHSGLICPTCEGGNSGEKSLSLFIAPDGSSATWNCFRGKCGLKG 180

Query: 181 HTLAFTDGRSSYKDLGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSV 240
              A  DG  +  D     +++  RKITVE ++LEPLCDE+ DYFA R IS++TL RN V
Sbjct: 181 GVRA--DGGLASAD----PIEKVERKITVEGIELEPLCDEIQDYFAARAISRKTLERNRV 240

Query: 241 MQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEG 300
           MQKR  ++I +AFTY++ G L+SCKYR   K F+QE KT +I YGLDDI+  S++IIVEG
Sbjct: 241 MQKRIGDEIVIAFTYWQRGELVSCKYRSLTKMFFQERKTRRILYGLDDIEKTSEVIIVEG 300

Query: 301 EIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVP 329
           EIDKL+M EAG  NCVSVPDGAP  VS K +P
Sbjct: 301 EIDKLAMEEAGFLNCVSVPDGAPAKVSSKEIP 319

[Close]

BLAST of CSPI01G25270 vs. Swiss-Prot
Match: PRIH_ARATH (Primase homolog protein OS=Arabidopsis thaliana GN=At1g30660 PE=3 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 2.6e-58
Identity = 132/260 (50.77%), Postives = 166/260 (63.85%), Query Frame = 1


Query: 70  MSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEAC 129
           MS   P     NG SS  S   VP   A       K  + ++L  L +KL +  ID + C
Sbjct: 1   MSENVPDHQSTNGFSS--SDFFVPEEEA------GKKVVLSKLVTLMRKLSEQGIDAQNC 60

Query: 130 VPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYK 189
            PG  S L+CP C+ GD+ E+S +L I  DG +A W C R KCG KG  +   DG+   K
Sbjct: 61  PPGVRSCLICPKCEVGDSGEKSLTLYIYPDGSSAKWTC-RRKCGLKG--VLQVDGKLVSK 120

Query: 190 D-LGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVA 249
           D +G+V      RKITVES++LEPLCDE+ D+FA R IS +TL RN VMQKR +++I +A
Sbjct: 121 DPIGKVE-----RKITVESIKLEPLCDEIQDFFAARAISGKTLERNRVMQKRIDDEIVIA 180

Query: 250 FTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGI 309
           FTY++ G L+SCKYR   K+F QE  T KI YGLDDI+  S+IIIVEGE DKL+M EAG 
Sbjct: 181 FTYWQRGELVSCKYRSLTKKFVQERNTRKILYGLDDIEETSEIIIVEGEPDKLAMEEAGF 240

Query: 310 HNCVSVPDGAPPSVSKKNVP 329
            NCVSVPDGAP +VS K +P
Sbjct: 241 FNCVSVPDGAPETVSSKEIP 244

[Close]

BLAST of CSPI01G25270 vs. TrEMBL
Match: A0A0A0LYM8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G541890 PE=4 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 9.2e-172
Identity = 306/333 (91.89%), Postives = 317/333 (95.20%), Query Frame = 1


Query: 1   MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSF---SQKYFLYRS 60
           MRFLH+NH LY PFSKLSSFSSP  LMGSFPLCKSTSLVFLSHLSSS    SQKYFLYRS
Sbjct: 1   MRFLHNNHCLYTPFSKLSSFSSPSCLMGSFPLCKSTSLVFLSHLSSSSSSSSQKYFLYRS 60

Query: 61  ISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRK 120
           ISLLHGSFPVRP+SLVKPFAMKPNGVSSFTSHANVPRPPALL NPPDKAS STRLNILRK
Sbjct: 61  ISLLHGSFPVRPLSLVKPFAMKPNGVSSFTSHANVPRPPALLENPPDKASSSTRLNILRK 120

Query: 121 KLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGH 180
           KLQDLDIDIEACVPGQF SLLCPMCKGGD++ERSFSLNISEDG AAVWNCFRGKCGWKGH
Sbjct: 121 KLQDLDIDIEACVPGQFYSLLCPMCKGGDSEERSFSLNISEDGGAAVWNCFRGKCGWKGH 180

Query: 181 TLAFTDGRSSYKDLGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVM 240
           TLAFTDGRSSYKDLGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVM
Sbjct: 181 TLAFTDGRSSYKDLGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVM 240

Query: 241 QKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGE 300
           QKRS+NQIAVAFTYYRGGALISCKYRDA K+FWQE  TE+IFYG+DDIDGASDIIIVEGE
Sbjct: 241 QKRSDNQIAVAFTYYRGGALISCKYRDANKKFWQEPNTERIFYGIDDIDGASDIIIVEGE 300

Query: 301 IDKLSMAEAGIHNCVSVPDGAPPSVSKKNVPPA 331
           +DKLSMAEAGIHNCVSVPDGAP SVS+K+VPPA
Sbjct: 301 MDKLSMAEAGIHNCVSVPDGAPASVSEKDVPPA 333

[Close]

BLAST of CSPI01G25270 vs. TrEMBL
Match: A0A0A0LVL8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G542410 PE=4 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 1.9e-145
Identity = 256/258 (99.22%), Postives = 256/258 (99.22%), Query Frame = 1


Query: 78  MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL 137
           MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL
Sbjct: 1   MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSL 60

Query: 138 LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALK 197
           LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAF DGRSSYK LGQVALK
Sbjct: 61  LCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFADGRSSYKHLGQVALK 120

Query: 198 QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL 257
           QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL
Sbjct: 121 QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGAL 180

Query: 258 ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG 317
           ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG
Sbjct: 181 ISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDG 240

Query: 318 APPSVSKKNVPPAHQVTL 336
           APPSVSKKNVPPAHQVTL
Sbjct: 241 APPSVSKKNVPPAHQVTL 258

[Close]

BLAST of CSPI01G25270 vs. TrEMBL
Match: A0A059CFW7_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D01628 PE=4 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 1.9e-84
Identity = 169/293 (57.68%), Postives = 204/293 (69.62%), Query Frame = 1


Query: 41  LSHLSSSFSQKYFLYRSISLLHGSFP-VRPMSLVKPFAMKPNGVSSFTSHANVPRPPALL 100
           LS  SSS S     +R    LH S    R  S + P  +K NGV     H+++PRP  L 
Sbjct: 53  LSSSSSSSSLSRVRFRGAYGLHFSCARSRAGSRMNPSCLKANGVPH-RPHSSIPRPVQLE 112

Query: 101 GNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISED 160
           G  P    I T L +L+ +L++L ID++ C+PGQ+ SLLCPMCKGG + ERS S+ +S D
Sbjct: 113 G--PVGRGIETPLKVLKMRLEELGIDMQTCLPGQYHSLLCPMCKGGRSMERSLSIFVSPD 172

Query: 161 GEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALKQNIRKITVESLQLEPLCDELVD 220
           G +AVW CFRG CGWKG T A    RSSY    Q +  + IR+I+ ESL LEPLC+ELV 
Sbjct: 173 GGSAVWTCFRGSCGWKGSTRAAAKPRSSYATSNQTSKVKTIREISEESLGLEPLCNELVA 232

Query: 221 YFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIF 280
           YFAERLIS +TL RN+VMQK+  NQI +AFTY R G L+SCKYRD KK+FWQEA TEKIF
Sbjct: 233 YFAERLISAETLRRNAVMQKKCENQIVIAFTYRREGKLVSCKYRDVKKKFWQEAHTEKIF 292

Query: 281 YGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ 333
           YGLDDI G S+IIIVEGE+DKL+M EAG  NCVSVPDGAPPS S K++PP  Q
Sbjct: 293 YGLDDIKGKSEIIIVEGEMDKLAMEEAGFRNCVSVPDGAPPSTSDKDLPPEGQ 342

[Close]

BLAST of CSPI01G25270 vs. TrEMBL
Match: A0A059CGG0_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D01628 PE=4 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 1.9e-84
Identity = 169/293 (57.68%), Postives = 204/293 (69.62%), Query Frame = 1


Query: 41  LSHLSSSFSQKYFLYRSISLLHGSFP-VRPMSLVKPFAMKPNGVSSFTSHANVPRPPALL 100
           LS  SSS S     +R    LH S    R  S + P  +K NGV     H+++PRP  L 
Sbjct: 53  LSSSSSSSSLSRVRFRGAYGLHFSCARSRAGSRMNPSCLKANGVPH-RPHSSIPRPVQLE 112

Query: 101 GNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISED 160
           G  P    I T L +L+ +L++L ID++ C+PGQ+ SLLCPMCKGG + ERS S+ +S D
Sbjct: 113 G--PVGRGIETPLKVLKMRLEELGIDMQTCLPGQYHSLLCPMCKGGRSMERSLSIFVSPD 172

Query: 161 GEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALKQNIRKITVESLQLEPLCDELVD 220
           G +AVW CFRG CGWKG T A    RSSY    Q +  + IR+I+ ESL LEPLC+ELV 
Sbjct: 173 GGSAVWTCFRGSCGWKGSTRAAAKPRSSYATSNQTSKVKTIREISEESLGLEPLCNELVA 232

Query: 221 YFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIF 280
           YFAERLIS +TL RN+VMQK+  NQI +AFTY R G L+SCKYRD KK+FWQEA TEKIF
Sbjct: 233 YFAERLISAETLRRNAVMQKKCENQIVIAFTYRREGKLVSCKYRDVKKKFWQEAHTEKIF 292

Query: 281 YGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ 333
           YGLDDI G S+IIIVEGE+DKL+M EAG  NCVSVPDGAPPS S K++PP  Q
Sbjct: 293 YGLDDIKGKSEIIIVEGEMDKLAMEEAGFRNCVSVPDGAPPSTSDKDLPPEGQ 342

[Close]

BLAST of CSPI01G25270 vs. TrEMBL
Match: W9RPH9_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_026953 PE=4 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 2.6e-81
Identity = 164/304 (53.95%), Postives = 217/304 (71.38%), Query Frame = 1


Query: 27  MGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAM-KPNGVSS 86
           MGS    KST   F S+  +  S +  L  +  L   +FP +P S  +P  + K NG SS
Sbjct: 1   MGSKQFLKST---FFSNPLTPASHRR-LSNTRKLPFSAFPSKPTSRTQPCCLIKTNGYSS 60

Query: 87  FTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGG 146
             S A+ PR   +L +P +K +  ++  IL++KL+DL ++ +  VPGQF+ L+CPMC GG
Sbjct: 61  -VSEASDPRA-VVLEDPEEKNA--SQFRILKQKLEDLGLECDISVPGQFNHLICPMCNGG 120

Query: 147 DTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALKQNIRKITV 206
           D +ERS SL I +DG +A+W CFR KCGW+G T AF + + +Y+   ++A  + IR+IT+
Sbjct: 121 DQEERSLSLFIEQDGSSALWVCFRAKCGWRGSTRAFAESKPAYERPNKIARIKKIREITI 180

Query: 207 ESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDA 266
           E L LEP CDE+V YF+ER+ISK+T+ RN+VMQKR ++Q A+AFTY+R G LISCKYRD 
Sbjct: 181 EDLGLEPPCDEIVAYFSERMISKETMQRNAVMQKRYDDQFAIAFTYWRNGNLISCKYRDI 240

Query: 267 KKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKK 326
            K+FWQEA TEKIFYGLDDI  ASDIIIVEGE+DKL+M EAG  NCVSVPDGAPP VS+K
Sbjct: 241 NKKFWQEADTEKIFYGLDDIKEASDIIIVEGEMDKLAMEEAGFRNCVSVPDGAPPCVSEK 296

Query: 327 NVPP 330
           ++PP
Sbjct: 301 DLPP 296

[Close]

BLAST of CSPI01G25270 vs. TAIR10
Match: AT1G30680.1 (AT1G30680.1 toprim domain-containing protein)

HSP 1 Score: 267.7 bits (683), Expect = 9.7e-72
Identity = 162/332 (48.80%), Postives = 208/332 (62.65%), Query Frame = 1


Query: 1   MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISL 60
           MRFL    +++  F KLS   S   LMGS    +   L   +   SS S  Y   R +S 
Sbjct: 1   MRFLLRLPQIH--FRKLSCSMSV--LMGSKQFLEFCLLPSFASYPSSPS--YSSSRQVSS 60

Query: 61  LHGSF-PV---RPMSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILR 120
           +   F PV   RP+S   P+  + NG+SS+ S   VP P        DK  + +RL  LR
Sbjct: 61  VSRRFRPVLASRPVSKNSPYYQRTNGLSSYNSIPRVPTPVDTEVEA-DKRVVLSRLVTLR 120

Query: 121 KKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKG 180
           +KL +  +D E C PGQ S L+CP C+GG++ E+S SL I+ DG +A WNCFRGKCG KG
Sbjct: 121 RKLAEQGVDAENCPPGQHSGLICPTCEGGNSGEKSLSLFIAPDGSSATWNCFRGKCGLKG 180

Query: 181 HTLAFTDGRSSYKDLGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSV 240
              A  DG  +  D     +++  RKITVE ++LEPLCDE+ DYFA R IS++TL RN V
Sbjct: 181 GVRA--DGGLASAD----PIEKVERKITVEGIELEPLCDEIQDYFAARAISRKTLERNRV 240

Query: 241 MQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEG 300
           MQKR  ++I +AFTY++ G L+SCKYR   K F+QE KT +I YGLDDI+  S++IIVEG
Sbjct: 241 MQKRIGDEIVIAFTYWQRGELVSCKYRSLTKMFFQERKTRRILYGLDDIEKTSEVIIVEG 300

Query: 301 EIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVP 329
           EIDKL+M EAG  NCVSVPDGAP  VS K +P
Sbjct: 301 EIDKLAMEEAGFLNCVSVPDGAPAKVSSKEIP 319

[Close]

BLAST of CSPI01G25270 vs. TAIR10
Match: AT1G30660.1 (AT1G30660.1 nucleic acid binding;nucleic acid binding)

HSP 1 Score: 227.3 bits (578), Expect = 1.5e-59
Identity = 132/260 (50.77%), Postives = 166/260 (63.85%), Query Frame = 1


Query: 70  MSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEAC 129
           MS   P     NG SS  S   VP   A       K  + ++L  L +KL +  ID + C
Sbjct: 1   MSENVPDHQSTNGFSS--SDFFVPEEEA------GKKVVLSKLVTLMRKLSEQGIDAQNC 60

Query: 130 VPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYK 189
            PG  S L+CP C+ GD+ E+S +L I  DG +A W C R KCG KG  +   DG+   K
Sbjct: 61  PPGVRSCLICPKCEVGDSGEKSLTLYIYPDGSSAKWTC-RRKCGLKG--VLQVDGKLVSK 120

Query: 190 D-LGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVA 249
           D +G+V      RKITVES++LEPLCDE+ D+FA R IS +TL RN VMQKR +++I +A
Sbjct: 121 DPIGKVE-----RKITVESIKLEPLCDEIQDFFAARAISGKTLERNRVMQKRIDDEIVIA 180

Query: 250 FTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGI 309
           FTY++ G L+SCKYR   K+F QE  T KI YGLDDI+  S+IIIVEGE DKL+M EAG 
Sbjct: 181 FTYWQRGELVSCKYRSLTKKFVQERNTRKILYGLDDIEETSEIIIVEGEPDKLAMEEAGF 240

Query: 310 HNCVSVPDGAPPSVSKKNVP 329
            NCVSVPDGAP +VS K +P
Sbjct: 241 FNCVSVPDGAPETVSSKEIP 244

[Close]

BLAST of CSPI01G25270 vs. NCBI nr
Match: gi|778662024|ref|XP_011659240.1| (PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial-like isoform X2 [Cucumis sativus])

HSP 1 Score: 666.4 bits (1718), Expect = 2.7e-188
Identity = 329/332 (99.10%), Postives = 330/332 (99.40%), Query Frame = 1


Query: 1   MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISL 60
           MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISL
Sbjct: 1   MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISL 60

Query: 61  LHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQ 120
           LHGSFPVRP+SLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQ
Sbjct: 61  LHGSFPVRPLSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQ 120

Query: 121 DLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLA 180
           DLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLA
Sbjct: 121 DLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLA 180

Query: 181 FTDGRSSYKDLGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKR 240
           F DGRSSYK LGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKR
Sbjct: 181 FADGRSSYKHLGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKR 240

Query: 241 SNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK 300
           SNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK
Sbjct: 241 SNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK 300

Query: 301 LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ 333
           LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ
Sbjct: 301 LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ 332

[Close]

BLAST of CSPI01G25270 vs. NCBI nr
Match: gi|778662022|ref|XP_011659237.1| (PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial-like isoform X1 [Cucumis sativus])

HSP 1 Score: 666.4 bits (1718), Expect = 2.7e-188
Identity = 329/332 (99.10%), Postives = 330/332 (99.40%), Query Frame = 1


Query: 1   MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISL 60
           MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISL
Sbjct: 1   MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISL 60

Query: 61  LHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQ 120
           LHGSFPVRP+SLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQ
Sbjct: 61  LHGSFPVRPLSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQ 120

Query: 121 DLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLA 180
           DLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLA
Sbjct: 121 DLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLA 180

Query: 181 FTDGRSSYKDLGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKR 240
           F DGRSSYK LGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKR
Sbjct: 181 FADGRSSYKHLGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKR 240

Query: 241 SNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK 300
           SNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK
Sbjct: 241 SNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK 300

Query: 301 LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ 333
           LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ
Sbjct: 301 LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ 332

[Close]

BLAST of CSPI01G25270 vs. NCBI nr
Match: gi|778662016|ref|XP_011659233.1| (PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial [Cucumis sativus])

HSP 1 Score: 610.9 bits (1574), Expect = 1.3e-171
Identity = 306/333 (91.89%), Postives = 317/333 (95.20%), Query Frame = 1


Query: 1   MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSF---SQKYFLYRS 60
           MRFLH+NH LY PFSKLSSFSSP  LMGSFPLCKSTSLVFLSHLSSS    SQKYFLYRS
Sbjct: 1   MRFLHNNHCLYTPFSKLSSFSSPSCLMGSFPLCKSTSLVFLSHLSSSSSSSSQKYFLYRS 60

Query: 61  ISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRK 120
           ISLLHGSFPVRP+SLVKPFAMKPNGVSSFTSHANVPRPPALL NPPDKAS STRLNILRK
Sbjct: 61  ISLLHGSFPVRPLSLVKPFAMKPNGVSSFTSHANVPRPPALLENPPDKASSSTRLNILRK 120

Query: 121 KLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGH 180
           KLQDLDIDIEACVPGQF SLLCPMCKGGD++ERSFSLNISEDG AAVWNCFRGKCGWKGH
Sbjct: 121 KLQDLDIDIEACVPGQFYSLLCPMCKGGDSEERSFSLNISEDGGAAVWNCFRGKCGWKGH 180

Query: 181 TLAFTDGRSSYKDLGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVM 240
           TLAFTDGRSSYKDLGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVM
Sbjct: 181 TLAFTDGRSSYKDLGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVM 240

Query: 241 QKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGE 300
           QKRS+NQIAVAFTYYRGGALISCKYRDA K+FWQE  TE+IFYG+DDIDGASDIIIVEGE
Sbjct: 241 QKRSDNQIAVAFTYYRGGALISCKYRDANKKFWQEPNTERIFYGIDDIDGASDIIIVEGE 300

Query: 301 IDKLSMAEAGIHNCVSVPDGAPPSVSKKNVPPA 331
           +DKLSMAEAGIHNCVSVPDGAP SVS+K+VPPA
Sbjct: 301 MDKLSMAEAGIHNCVSVPDGAPASVSEKDVPPA 333

[Close]

BLAST of CSPI01G25270 vs. NCBI nr
Match: gi|659068706|ref|XP_008445781.1| (PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 568.9 bits (1465), Expect = 5.8e-159
Identity = 288/347 (83.00%), Postives = 305/347 (87.90%), Query Frame = 1


Query: 1   MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSS------FSQKYFL 60
           MRFLHHN  LYNPFSKLSSFSSPF LMGSFPLCKSTSLVFLSHLSSS      +SQKYFL
Sbjct: 1   MRFLHHNQCLYNPFSKLSSFSSPFCLMGSFPLCKSTSLVFLSHLSSSSSSSSSYSQKYFL 60

Query: 61  YRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNI 120
           YRSISLLHGSFPVRP SLVKPFAMKPNG SSFTSHANVPRPPA   NP DKA  ST LNI
Sbjct: 61  YRSISLLHGSFPVRPTSLVKPFAMKPNGFSSFTSHANVPRPPAFSENPRDKALSSTLLNI 120

Query: 121 LRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGW 180
           LRKKLQDL IDIEACVPGQFS LLCPMCKGGD+KERSF+L+ISEDG AAVWNCFRGKCGW
Sbjct: 121 LRKKLQDLGIDIEACVPGQFSGLLCPMCKGGDSKERSFALHISEDGGAAVWNCFRGKCGW 180

Query: 181 KGHTLAFTDGRSSY---------KDLGQVALKQNIRKITVESLQLEPLCDELVDYFAERL 240
           KGHTLA  + RSSY         +DLG+VALKQNIRKITVESLQLEPLCD++V YFAERL
Sbjct: 181 KGHTLALAEDRSSYQDLGRVALKQDLGRVALKQNIRKITVESLQLEPLCDQVVAYFAERL 240

Query: 241 ISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDI 300
           ISK+TLLRNSVMQK   +QIA+AFTYYRGGAL+SCKYRD  K+FWQEA TEKIFYG+DDI
Sbjct: 241 ISKETLLRNSVMQKTFGDQIAIAFTYYRGGALVSCKYRDVNKKFWQEANTEKIFYGIDDI 300

Query: 301 DGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ 333
           +GASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKK+VP A Q
Sbjct: 301 EGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKDVPSADQ 347

[Close]

BLAST of CSPI01G25270 vs. NCBI nr
Match: gi|659068709|ref|XP_008445786.1| (PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial isoform X2 [Cucumis melo])

HSP 1 Score: 568.9 bits (1465), Expect = 5.8e-159
Identity = 288/347 (83.00%), Postives = 305/347 (87.90%), Query Frame = 1


Query: 1   MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSS------FSQKYFL 60
           MRFLHHN  LYNPFSKLSSFSSPF LMGSFPLCKSTSLVFLSHLSSS      +SQKYFL
Sbjct: 1   MRFLHHNQCLYNPFSKLSSFSSPFCLMGSFPLCKSTSLVFLSHLSSSSSSSSSYSQKYFL 60

Query: 61  YRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNI 120
           YRSISLLHGSFPVRP SLVKPFAMKPNG SSFTSHANVPRPPA   NP DKA  ST LNI
Sbjct: 61  YRSISLLHGSFPVRPTSLVKPFAMKPNGFSSFTSHANVPRPPAFSENPRDKALSSTLLNI 120

Query: 121 LRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGW 180
           LRKKLQDL IDIEACVPGQFS LLCPMCKGGD+KERSF+L+ISEDG AAVWNCFRGKCGW
Sbjct: 121 LRKKLQDLGIDIEACVPGQFSGLLCPMCKGGDSKERSFALHISEDGGAAVWNCFRGKCGW 180

Query: 181 KGHTLAFTDGRSSY---------KDLGQVALKQNIRKITVESLQLEPLCDELVDYFAERL 240
           KGHTLA  + RSSY         +DLG+VALKQNIRKITVESLQLEPLCD++V YFAERL
Sbjct: 181 KGHTLALAEDRSSYQDLGRVALKQDLGRVALKQNIRKITVESLQLEPLCDQVVAYFAERL 240

Query: 241 ISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDI 300
           ISK+TLLRNSVMQK   +QIA+AFTYYRGGAL+SCKYRD  K+FWQEA TEKIFYG+DDI
Sbjct: 241 ISKETLLRNSVMQKTFGDQIAIAFTYYRGGALVSCKYRDVNKKFWQEANTEKIFYGIDDI 300

Query: 301 DGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ 333
           +GASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKK+VP A Q
Sbjct: 301 EGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKDVPSADQ 347

The following BLAST results are available for this feature:

BLAST of CSPI01G25270 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TWIH_ARATH	1.7e-70	48.80	Twinkle homolog protein, chloroplastic/mitochondrial OS=Arabidopsis thaliana GN=...	[more]
PRIH_ARATH	2.6e-58	50.77	Primase homolog protein OS=Arabidopsis thaliana GN=At1g30660 PE=3 SV=1	[more]

BLAST of CSPI01G25270 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LYM8_CUCSA	9.2e-172	91.89	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G541890 PE=4 SV=1	[more]
A0A0A0LVL8_CUCSA	1.9e-145	99.22	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G542410 PE=4 SV=1	[more]
A0A059CFW7_EUCGR	1.9e-84	57.68	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D01628 PE=4 SV=1	[more]
A0A059CGG0_EUCGR	1.9e-84	57.68	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D01628 PE=4 SV=1	[more]
W9RPH9_9ROSA	2.6e-81	53.95	Uncharacterized protein OS=Morus notabilis GN=L484_026953 PE=4 SV=1	[more]

BLAST of CSPI01G25270 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G30680.1	9.7e-72	48.80	toprim domain-containing protein	[more]
AT1G30660.1	1.5e-59	50.77	nucleic acid binding;nucleic acid binding	[more]

BLAST of CSPI01G25270 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778662024\|ref\|XP_011659240.1\|	2.7e-188	99.10	PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial-like isoform X2 ...	[more]
gi\|778662022\|ref\|XP_011659237.1\|	2.7e-188	99.10	PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial-like isoform X1 ...	[more]
gi\|778662016\|ref\|XP_011659233.1\|	1.3e-171	91.89	PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial [Cucumis sativus...	[more]
gi\|659068706\|ref\|XP_008445781.1\|	5.8e-159	83.00	PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial isoform X1 [Cucu...	[more]
gi\|659068709\|ref\|XP_008445786.1\|	5.8e-159	83.00	PREDICTED: twinkle homolog protein, chloroplastic/mitochondrial isoform X2 [Cucu...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006171	TOPRIM_domain
IPR027032	Twinkle-like protein

Vocabulary: **Molecular Function**
Term	Definition
GO:0003697	single-stranded DNA binding
GO:0043139	5'-3' DNA helicase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032508	DNA duplex unwinding
biological_process	GO:0006260	DNA replication
cellular_component	GO:0005657	replication fork
molecular_function	GO:0043139	5'-3' DNA helicase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003697	single-stranded DNA binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G25270.1	CSPI01G25270.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006171	Toprim domain	PFAM	PF13662	Toprim_4	coord: 290..323 score: 6.
IPR027032	Twinkle-like protein	PANTHER	PTHR12873	T7-LIKE MITOCHONDRIAL DNA HELICASE	coord: 109..323 score: 1.8
None	No IPR available	GENE3D	G3DSA:3.40.1360.10		coord: 276..323 score: 1.5
None	No IPR available	PANTHER	PTHR12873:SF2	SUBFAMILY NOT NAMED	coord: 109..323 score: 1.8
None	No IPR available	unknown	SSF56731	DNA primase core	coord: 221..320 score: 2.35

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI01G25270	Cla019748	Watermelon (97103) v1	cpiwmB016

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI01G25270	Wax gourd	cpiwgoB054
CSPI01G25270	Cucumber (Gy14) v1	cgycpiB262
CSPI01G25270	Cucurbita maxima (Rimu)	cmacpiB221
CSPI01G25270	Cucurbita maxima (Rimu)	cmacpiB396
CSPI01G25270	Cucurbita moschata (Rifu)	cmocpiB045
CSPI01G25270	Cucurbita moschata (Rifu)	cmocpiB207
CSPI01G25270	Cucurbita moschata (Rifu)	cmocpiB384
CSPI01G25270	Cucumber (Chinese Long) v2	cpicuB001
CSPI01G25270	Melon (DHL92) v3.5.1	cpimeB028
CSPI01G25270	Watermelon (Charleston Gray)	cpiwcgB044
CSPI01G25270	Watermelon (Charleston Gray)	cpiwcgB045
CSPI01G25270	Cucurbita pepo (Zucchini)	cpecpiB026
CSPI01G25270	Cucurbita pepo (Zucchini)	cpecpiB508
CSPI01G25270	Bottle gourd (USVL1VR-Ls)	cpilsiB022
CSPI01G25270	Melon (DHL92) v3.6.1	cpimedB027
CSPI01G25270	Cucumber (Gy14) v2	cgybcpiB002
CSPI01G25270	Silver-seed gourd	carcpiB0430
CSPI01G25270	Silver-seed gourd	carcpiB0485
CSPI01G25270	Cucumber (Chinese Long) v3	cpicucB000
CSPI01G25270	Watermelon (97103) v2	cpiwmbB039