CSPI01G25200 (gene) Wild cucumber (PI 183967)

BLAST of CSPI01G25200 vs. Swiss-Prot
Match: THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 6.0e-142
Identity = 258/516 (50.00%), Postives = 343/516 (66.47%), Query Frame = 1

Query: 33  KDFLQCLSIHSTHIP--------IYTPINSSYSSILRSYAQNPRFSTQYNTLKPLVIIKP 92
           ++FL+C S    HIP        +YT  +  Y SIL S  QN RF +   T KPLVI+ P
Sbjct: 32  ENFLKCFS---KHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISD-TTPKPLVIVTP 91

Query: 93  SHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFVVVDLINLKSIEVDTTNNTA 152
           S+ SH+Q+T++CSK  GLQIR RSGGHD EG+SYIS +PFVVVDL N+ SI++D  + TA
Sbjct: 92  SNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTA 151

Query: 153 WVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDA 212
           WV++GAT+GE+YY I EK+  L+FP G CPTVG+GGHFSGGGYG LMR YGLAADN+IDA
Sbjct: 152 WVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDA 211

Query: 213 YLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLD-K 272
           +LV+ +G V DR++MGE LFWAIRGGGG +FGI+  WK+KLV VP   T   + K+++  
Sbjct: 212 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIH 271

Query: 273 NTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPT---ASFFSLYLGKTDKLVTI 332
              K+  +WQ +A + ++ L++  +    N T    +N T     F S++ G  D LV +
Sbjct: 272 GLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDL 331

Query: 333 MNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPL----EILLSKPTLSNNISYKIKSD 392
           MN + P LG+ K +CKE SWI +T+  +G  N        EILL + +     ++ IK D
Sbjct: 332 MNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDR-SAGKKTAFSIKLD 391

Query: 393 YVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKTPFSHRAEFLYKISYT 452
           YVK+PI + A   I ++L  ++V      L+PYGG M  IS S  PF HRA  +Y++ YT
Sbjct: 392 YVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYT 451

Query: 453 VGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLDIGTNNKYGKTSYEEAS 512
             W +Q    NE+H+NW+R  Y+F TP+VS +PR AY+NYRDLD+G  N     +Y +A 
Sbjct: 452 ASWEKQED--NEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQAR 511

Query: 513 IWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPL 533
           IWG KYFG NF RLV+VKT VDP+NFFR+EQSIPPL
Sbjct: 512 IWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of CSPI01G25200 vs. Swiss-Prot
Match: CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa GN=CBDAS PE=1 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 5.1e-141
Identity = 261/530 (49.25%), Postives = 353/530 (66.60%), Query Frame = 1

Query: 16  IVSLFSTQWSTASSYKSKDFLQCLSIH----STHIP-IYTPINSSYSSILRSYAQNPRFS 75
           I   FS    T+ +   ++FL+C S +    +T++  +YT  N  Y S+L S   N RF+
Sbjct: 15  IFFFFSFNIQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFT 74

Query: 76  TQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFVVVDLI 135
           +   T KPLVI+ PSHVSH+Q T++CSK  GLQIR RSGGHD+EG+SYIS +PFV+VDL 
Sbjct: 75  SD-TTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLR 134

Query: 136 NLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFSGGGYGWL 195
           N++SI++D  + TAWV++GAT+GE+YY + EK+  L+  AG CPTV  GGHF GGGYG L
Sbjct: 135 NMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPL 194

Query: 196 MRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKLVRVPP 255
           MR YGLAADN+IDA+LV+ +G V DR++MGE LFWA+RGGG  SFGI+V WK++LV VP 
Sbjct: 195 MRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPK 254

Query: 256 IVTTCRLDKSLD-KNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPTA---SF 315
             T   + K ++     K+V +WQ +A + ++ LL+  +    N T    +N TA    F
Sbjct: 255 -STMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYF 314

Query: 316 FSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQP----LEILLSK 375
            S++LG  D LV +MN + P LG+ K +C++ SWI + +  +G  N        EILL +
Sbjct: 315 SSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLDR 374

Query: 376 PTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKTP 435
            +   N ++KIK DYVK+PI +  F  I ++L  +++      L+PYGG M  IS S  P
Sbjct: 375 -SAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIP 434

Query: 436 FSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLDIG 495
           F HRA  LY++ Y   W +Q    NE+HLNWIR  Y+FMTP+VS +PR AY+NYRDLDIG
Sbjct: 435 FPHRAGILYELWYICSWEKQED--NEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIG 494

Query: 496 TNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPL 533
            N+     +Y +A IWG KYFG NF RLV+VKT VDP+NFFR+EQSIPPL
Sbjct: 495 INDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 539

BLAST of CSPI01G25200 vs. Swiss-Prot
Match: CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 3.3e-140
Identity = 260/528 (49.24%), Postives = 349/528 (66.10%), Query Frame = 1

Query: 20  FSTQWSTASSYKSKDFLQCLSIH-STHIP----IYTPINSSYSSILRSYAQNPRFSTQYN 79
           F+ Q S A+    ++FL+C S +  T++     +YT  +  Y SIL S  QN RF++   
Sbjct: 21  FNIQISIANP--QENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSD-T 80

Query: 80  TLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFVVVDLINLKS 139
           T KPLVII P +VSH+Q T++CSK  GLQIR RSGGHD EG+SYIS +PFV+VDL N+ S
Sbjct: 81  TPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHS 140

Query: 140 IEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFSGGGYGWLMRKY 199
           +++D  + TAWV++GAT+GE+YY I E +  L+FPAG CPTVG GGHFSGGGYG LMR Y
Sbjct: 141 VKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNY 200

Query: 200 GLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKLVRVPPIVTT 259
           GLAADN+IDA+LV+ +G V DR++MGE LFWAIRGGGG +FGI+  WK++LV VP + T 
Sbjct: 201 GLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTI 260

Query: 260 CRLDKSLD-KNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPT---ASFFSLY 319
             + K+++     K+V +WQ +A   E++LL+  +    N T    +N T   + F S++
Sbjct: 261 FSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIF 320

Query: 320 LGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPL----EILLSKPTLS 379
            G  D LV +MN + P LG+ K +CK+ SWI + +  +G  N        EILL + +  
Sbjct: 321 HGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDR-SGG 380

Query: 380 NNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKTPFSHR 439
              ++ IK DYVK+PI + A   I ++L  ++V       +PYGG M  IS S  PF HR
Sbjct: 381 RKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHR 440

Query: 440 AEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLDIGTNNK 499
           A  +Y+I Y   W +Q    NE+H+NWIR  Y+F TP+VS +PR AY+NYRDLD+G  N 
Sbjct: 441 AGIMYEIWYIASWEKQED--NEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNF 500

Query: 500 YGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTL 535
               +Y +A IWG KYFG NF RLV+VKT VDP NFFR+EQSIPPL L
Sbjct: 501 ESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLPL 542

BLAST of CSPI01G25200 vs. Swiss-Prot
Match: CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 3.3e-140
Identity = 261/528 (49.43%), Postives = 348/528 (65.91%), Query Frame = 1

Query: 20  FSTQWSTASSYKSKDFLQCLSIH-STHIP----IYTPINSSYSSILRSYAQNPRFSTQYN 79
           F+ Q S A+    ++FL+CLS +  T++     +YT  +  Y SIL S  QN RF++   
Sbjct: 21  FNIQISIANP--QENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSD-T 80

Query: 80  TLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFVVVDLINLKS 139
           T KPLVI  P +VSH+Q T++CSK  GLQIR RSGGHD EG+SYIS +PFV+VDL N+ S
Sbjct: 81  TPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHS 140

Query: 140 IEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFSGGGYGWLMRKY 199
           +++D  + TAWV+SGAT+GE+YY I E +  L+FPAG CPTVG GGHFSGGGYG LMR Y
Sbjct: 141 VKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNY 200

Query: 200 GLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKLVRVPPIVTT 259
           GLAADN+IDA+LV+ +G V DR++MGE LFWAIRGGGG +FGI+  WK++LV VP + T 
Sbjct: 201 GLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTI 260

Query: 260 CRLDKSLD-KNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPT---ASFFSLY 319
             + K+++     K+V +WQ +A   E++LL+  +    N T    +N T   + F S++
Sbjct: 261 FSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIF 320

Query: 320 LGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPL----EILLSKPTLS 379
            G  D LV +MN + P LG+ K +CK+ SWI + +  +G  N        EILL + +  
Sbjct: 321 HGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDR-SGG 380

Query: 380 NNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKTPFSHR 439
              ++ IK DYVK+PI + A   I ++L  ++V       +PYGG M  IS S  PF HR
Sbjct: 381 RKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHR 440

Query: 440 AEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLDIGTNNK 499
           A   Y+I Y   W +Q    NE+H+NWIR  Y+F TP+VS +PR AY+NYRDLD+G  N 
Sbjct: 441 AGITYEIWYIASWEKQED--NEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNF 500

Query: 500 YGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTL 535
               +Y +A IWG KYFG NF RLV+VKT VDP NFFR+EQSIPPL L
Sbjct: 501 ESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLPL 542

BLAST of CSPI01G25200 vs. Swiss-Prot
Match: THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 5.6e-140
Identity = 256/526 (48.67%), Postives = 347/526 (65.97%), Query Frame = 1

Query: 20  FSTQWSTASSYKSKDFLQCLSIHSTHIP-----IYTPINSSYSSILRSYAQNPRFSTQYN 79
           F+ Q S A+    ++FL+C S +  + P     IYT  +  Y S+L S  QN RF++   
Sbjct: 21  FNIQISIANP--QENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSD-T 80

Query: 80  TLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFVVVDLINLKS 139
           T KPLVI+ PS+VSH+Q++++CSK  GLQIR RSGGHD EGLSYIS +PF +VDL N+ +
Sbjct: 81  TPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHT 140

Query: 140 IEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFSGGGYGWLMRKY 199
           ++VD  + TAWV++GAT+GE+YY I E +   +FP G CPTVG+GGHFSGGGYG LMR Y
Sbjct: 141 VKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNY 200

Query: 200 GLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKLVRVPPIVTT 259
           GLAADN+IDA+LV+ +G V DR++MGE LFWAIRGGGG +FGI+  WK+KLV VP   T 
Sbjct: 201 GLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATI 260

Query: 260 CRLDKSLD-KNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPT---ASFFSLY 319
             + K+++     K+  +WQ +A + ++ L++  +    N T    +N T     F S++
Sbjct: 261 FSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIF 320

Query: 320 LGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPL----EILLSKPTLS 379
           LG  D LV +MN + P LG+ K +CKE SWI +T+  +G  N        EILL + +  
Sbjct: 321 LGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDR-SAG 380

Query: 380 NNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKTPFSHR 439
              ++ IK DYVK+ I + A   I ++L  +EV      L+PYGG M  IS S  PF HR
Sbjct: 381 KKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHR 440

Query: 440 AEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLDIGTNNK 499
           A  +Y++ YT  W +Q    NE+H+NW+R  Y+F TP+VS +PR AY+NYRDLD+G  N 
Sbjct: 441 AGIMYELWYTATWEKQED--NEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNP 500

Query: 500 YGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPL 533
               +Y +A IWG KYFG NF RLV+VKT  DP+NFFR+EQSIPPL
Sbjct: 501 ESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of CSPI01G25200 vs. TrEMBL
Match: B9NA97_POPTR (FAD-binding domain-containing family protein OS=Populus trichocarpa GN=POPTR_0001s43120g PE=4 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 9.2e-174
Identity = 303/528 (57.39%), Postives = 388/528 (73.48%), Query Frame = 1

Query: 11  LISFTIVSLFSTQWSTASSYKSKDFLQCLSIHSTHIP---IYTPINSSYSSILRSYAQNP 70
           ++SF    +FS  W T S++  + FLQCL   ++H     IYTPINSSYSSIL+   QN 
Sbjct: 1   MLSFLFFLIFSFSWVT-SAHTHESFLQCLDSQNSHSISKLIYTPINSSYSSILQFSIQNL 60

Query: 71  RFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFVVV 130
           RF+T  +T KPLVI+ P+++SH+Q+ +ICS+ HG+QIRIRSGGHD EGLSY+S   FVV+
Sbjct: 61  RFNTS-STPKPLVIVTPTNISHIQAAIICSQKHGMQIRIRSGGHDYEGLSYVSTFSFVVI 120

Query: 131 DLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFSGGGY 190
           DLINL++I V+  N TAWVQSG TIGE+YYRIAEKSRTLAFPA VCPTVG+GGHFSGGGY
Sbjct: 121 DLINLRTINVNAENKTAWVQSGVTIGEVYYRIAEKSRTLAFPASVCPTVGVGGHFSGGGY 180

Query: 191 GWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKLVR 250
           G LMRKYGLAAD++IDA LVD  G + DRE+MGE LFWAIRGGGG +FG+V+ WK+ LV 
Sbjct: 181 GMLMRKYGLAADHIIDAQLVDVKGRILDRESMGEDLFWAIRGGGGNTFGVVIAWKISLVS 240

Query: 251 VPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPT--AS 310
            PP VT   + ++L++N  K+V++WQ+VA+++ E L I I L   N T+  K N T  AS
Sbjct: 241 APPTVTVFNVQRTLEQNASKLVHRWQFVADKLHEDLFIRIILNRVNSTEEGKSNTTIQAS 300

Query: 311 FFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLSKPTL 370
           F SL+LG  D+L+ ++  + P LGL K +C E SWIQS L   GF +   L++LL + T 
Sbjct: 301 FNSLFLGGIDRLLPLIQDSFPELGLVKEDCIEMSWIQSVLYFDGFPSNSSLDVLLDR-TP 360

Query: 371 SNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKTPFSH 430
           S   ++K KSDYVK+PI +   +GIW+R   +++ T  L   PYGGKM  IS S  PF H
Sbjct: 361 STRRNFKAKSDYVKEPIPELGLEGIWERFFDKDINTPILIFSPYGGKMSEISESSIPFPH 420

Query: 431 RAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLDIGTNN 490
           RA  +YKI + + W E+G  A +RH++WIR  YS++ P+VS +PRAAY+NYRDLDIG NN
Sbjct: 421 RAGNIYKIQHLIYWDEEGIVATKRHISWIRRLYSYLAPYVSKTPRAAYVNYRDLDIGINN 480

Query: 491 KYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLT 534
             G TSY +ASIWGLKYF NNF RLV+VKT VDP+NFFR+EQSIPPL+
Sbjct: 481 HAGNTSYRQASIWGLKYFKNNFDRLVRVKTAVDPANFFRNEQSIPPLS 525

BLAST of CSPI01G25200 vs. TrEMBL
Match: U5FWX5_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s16570g PE=4 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 1.2e-170
Identity = 295/528 (55.87%), Postives = 387/528 (73.30%), Query Frame = 1

Query: 11  LISFTIVSLFSTQWSTASSYKSKDFLQCLSIHS-----THIPIYTPINSSYSSILRSYAQ 70
           ++ F ++ LF   W T S++  +DFLQCLS++S     T   IYTP N+SY+++L+   +
Sbjct: 8   MLPFLLILLFPFSWGT-SAHTHEDFLQCLSLYSEDSAATSKVIYTPNNTSYATVLQFSIR 67

Query: 71  NPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFV 130
           N RF++  + L P+VI+ P+ +SH+Q+T+ CS+ H LQIRIRSGGHD EGLSY+S LPFV
Sbjct: 68  NLRFNS--SKLTPVVIVTPTILSHIQATIHCSQKHNLQIRIRSGGHDYEGLSYMSVLPFV 127

Query: 131 VVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFSGG 190
           ++DLINL+ I VD +  TAWVQ+GAT+GELYY IAEKSRTLAFPAG C TVG+GG FSGG
Sbjct: 128 ILDLINLRKITVDLSTKTAWVQAGATLGELYYSIAEKSRTLAFPAGACHTVGVGGQFSGG 187

Query: 191 GYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKL 250
           GYG L+RKYGLAADNVIDA L+DANG V DRE+MGE LFWAIRGGGG SFG+V  WKV L
Sbjct: 188 GYGGLLRKYGLAADNVIDAELIDANGRVLDRESMGEDLFWAIRGGGGNSFGVVTAWKVNL 247

Query: 251 VRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPTAS 310
           V VPP VT   + K L +N  K++++WQYVAN++ E ++I   +   N ++G      A+
Sbjct: 248 VEVPPTVTVFAVPKVLKENATKLIHRWQYVANKLPEDIVIAAYVNRINSSQGGNPTIQAT 307

Query: 311 FFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLSKPTL 370
           F SL+LG  D+L+ +M  + P LGL K +C E SWI+  L  AGF +   L++LL + T 
Sbjct: 308 FTSLFLGGVDRLLPLMQESFPELGLVKDDCIELSWIEFALFLAGFPSNASLDVLLDR-TP 367

Query: 371 SNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKTPFSH 430
            +  S+K KSDYVKQP+ + A +G+W+    +++E+  LF+ PYGGKM  IS S  PF H
Sbjct: 368 QSITSFKAKSDYVKQPLPETALEGMWETFFEKDIESPSLFMVPYGGKMEEISESSIPFPH 427

Query: 431 RAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLDIGTNN 490
           RA  LYKI Y V W E+G  A+ERH++WIR  YS+MTP+VS +PR AY+NYRDLD+G NN
Sbjct: 428 RAGNLYKIHYYVAWTEEGKEASERHISWIRRLYSYMTPYVSKNPREAYVNYRDLDLGINN 487

Query: 491 KYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLT 534
             G TSY++ASIWG KYF NNF +LV++KT VDP+NFFR+EQSIPP +
Sbjct: 488 LAGNTSYKQASIWGRKYFKNNFDKLVRIKTEVDPANFFRNEQSIPPFS 531

BLAST of CSPI01G25200 vs. TrEMBL
Match: U5G2R0_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s16560g PE=4 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 3.6e-170
Identity = 296/528 (56.06%), Postives = 388/528 (73.48%), Query Frame = 1

Query: 11  LISFTIVSLFSTQWSTASSYKSKDFLQCLSIH---STHIP--IYTPINSSYSSILRSYAQ 70
           ++ F +  LFS    T S+   +DFLQCLS+H   ST I   IYTP NSSYSSIL    +
Sbjct: 8   MLPFLLCLLFSLSRVT-SARPHEDFLQCLSLHFEDSTAISKVIYTPKNSSYSSILHFAIR 67

Query: 71  NPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFV 130
           NPRF++  + LKP VI+ P+  SH+Q+ + CS+ H L+IRIRSGGHD EGLSY+S +PFV
Sbjct: 68  NPRFNS--SELKPFVIVTPTDASHIQAAIHCSQKHKLEIRIRSGGHDFEGLSYMSTVPFV 127

Query: 131 VVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFSGG 190
           +VDLINL+SI VD TN TAWVQ+GAT+GELYYRIAEKSRTLAFPAG CPT+G+GGHFSGG
Sbjct: 128 IVDLINLRSITVDATNKTAWVQAGATLGELYYRIAEKSRTLAFPAGSCPTIGVGGHFSGG 187

Query: 191 GYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKL 250
           GY  + RKYGLA+DNVIDA L+DA G + DRE+MGE LFWAIRGGGG SFG+V+ W+++L
Sbjct: 188 GYSTISRKYGLASDNVIDAQLIDAKGRILDRESMGEDLFWAIRGGGGQSFGVVIAWRIEL 247

Query: 251 VRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPTAS 310
           V VPP VT     ++L++N  K++++WQYVAN++ E ++I + +   N ++  K    A+
Sbjct: 248 VEVPPKVTVFTAARTLEQNATKLIHRWQYVANQLPEDIIIDVLVNRVNSSEEGKSTIQAA 307

Query: 311 FFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLSKPTL 370
           FFSL+LG+ D+L+ +M  + P LGL K  C E SWI+S +   GF +   L +LL +   
Sbjct: 308 FFSLFLGEVDQLLLLMQESFPELGLAKDECTEMSWIESVIYIIGFPSNASLNVLLDRTPQ 367

Query: 371 SNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKTPFSH 430
             ++ +K KSDYV++PI + AF+GIW R   +++E  + F+ PYGGKM  IS S TPF H
Sbjct: 368 PPSLQFKAKSDYVQEPIPEIAFEGIWKRFFEKDIEVPEFFMLPYGGKMDEISESSTPFPH 427

Query: 431 RAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLDIGTNN 490
           RA   Y ++  V W+E+   A++RHL WIR  Y +MTP+VS +PRAAY+NYRDLD+G NN
Sbjct: 428 RAGNRYIVAPVVYWSEETKEASQRHLAWIRRLYRYMTPYVSKNPRAAYVNYRDLDLGVNN 487

Query: 491 KYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLT 534
             G TSY++ASIWG KYF NNF RLV+VKT VDP+NFFR+EQSIPPL+
Sbjct: 488 -LGYTSYKQASIWGRKYFKNNFDRLVRVKTEVDPTNFFRNEQSIPPLS 531

BLAST of CSPI01G25200 vs. TrEMBL
Match: B9GH03_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s46900g PE=4 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 1.7e-167
Identity = 294/523 (56.21%), Postives = 381/523 (72.85%), Query Frame = 1

Query: 16  IVSLFSTQWSTASSYKSKDFLQCLSIH-----STHIPIYTPINSSYSSILRSYAQNPRFS 75
           +V L S+     SS+  +DFL+CLS++     +    +YT  NSSYSS+L    +N RF+
Sbjct: 12  LVFLLSSSSLATSSHTHEDFLRCLSLYFEDPVAISKVVYTSNNSSYSSVLHFSIRNLRFN 71

Query: 76  TQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYISDLPFVVVDLI 135
           +  +TLKPLVI+ P+ +SH+Q+T+ CS+ H LQIR+RSGGHD EGLSY S LPFVVVDLI
Sbjct: 72  S--STLKPLVIVTPTDLSHIQATIHCSRHHNLQIRMRSGGHDFEGLSYRSVLPFVVVDLI 131

Query: 136 NLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFSGGGYGWL 195
           NL++I VD TN TAWVQ+GAT+GELYYRI EKSRTLAFPAG CPTVG+GGHFSGGGYG L
Sbjct: 132 NLRTITVDATNRTAWVQAGATLGELYYRIVEKSRTLAFPAGSCPTVGVGGHFSGGGYGLL 191

Query: 196 MRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKVKLVRVPP 255
           +RKYGLAADN++DA L+DA G + DRE+MG+ LFWAIRGGGG SFG+VV WK+ LV VP 
Sbjct: 192 LRKYGLAADNIVDAQLIDAKGRILDRESMGDDLFWAIRGGGGNSFGVVVAWKINLVEVPS 251

Query: 256 IVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPTASFFSLY 315
            VT   + ++L++N  K+++QWQYVAN++ E L +   +   N ++G      A F SL+
Sbjct: 252 TVTVFTVVRNLEQNATKLIHQWQYVANKLPEDLFLAAVIRRVNSSQGGNTAVQAIFVSLF 311

Query: 316 LGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLSKPTLSNNIS 375
           LGK D+L+  M+ + P+LG+ + +C E SWI+S L   GF     L++LL + + S  I+
Sbjct: 312 LGKVDQLLPWMHGSFPDLGIVRDDCIEMSWIESILYVYGFPRNASLDVLLDRSSQS-LIN 371

Query: 376 YKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKTPFSHRAEFL 435
           +K+KSDYVK+P+++   K IW+R   + +E S +   PYGGKM  IS S  PF HRA  L
Sbjct: 372 FKVKSDYVKEPMAEIVLKEIWERFSDENMEVSAMSFIPYGGKMNKISESSIPFPHRAGNL 431

Query: 436 YKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLDIGTNNKYGKT 495
           YKI +TV W+E+   A+ERHL WIR  Y +MT +VS  PR AYINYRDLDIG NN  G T
Sbjct: 432 YKIIHTVAWSEE--TASERHLAWIRRLYGYMTSYVSQKPREAYINYRDLDIGMNNPVGST 491

Query: 496 SYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLT 534
           SY +ASIWG KYF NNF +LV+VKT VDP+NFFR+EQSIPPL+
Sbjct: 492 SYGQASIWGRKYFKNNFDKLVRVKTEVDPANFFRNEQSIPPLS 529

BLAST of CSPI01G25200 vs. TrEMBL
Match: B9N5Q8_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s16580g PE=4 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 2.2e-167
Identity = 297/533 (55.72%), Postives = 381/533 (71.48%), Query Frame = 1

Query: 6   LNPLFLISFTIVSLFSTQWSTASSYKSKDFLQCLSIHSTHIP-----IYTPINSSYSSIL 65
           L+   ++ F    LFS  WS AS++  +DFLQCLS++S         +YTP NSSY  +L
Sbjct: 5   LSTTTMLPFLCFLLFSFLWS-ASAHTHEDFLQCLSLYSEDSNSFSKLVYTPNNSSYLHVL 64

Query: 66  RSYAQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 125
           +   QN RF+T   T KP VI+ P +VSH+Q+T+ CS+ HG+ +RIRSGGHD EGLSY+S
Sbjct: 65  QGSIQNSRFNTTA-TPKPPVIVTPMNVSHIQATIFCSQKHGMNVRIRSGGHDYEGLSYVS 124

Query: 126 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG 185
            LPFV++DLINL++I VD  N+TAWVQ+GAT+GELYY IAEKS TLAFPAG CPTVG GG
Sbjct: 125 VLPFVIIDLINLRAINVDVENSTAWVQAGATLGELYYSIAEKSGTLAFPAGACPTVGAGG 184

Query: 186 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVG 245
           H SGGGYG LMRKYGLAADN+IDA L+DA G + DR +MGE LFWAIRGGGG +FG+VV 
Sbjct: 185 HLSGGGYGGLMRKYGLAADNIIDAQLIDAKGRILDRASMGEDLFWAIRGGGGNTFGVVVA 244

Query: 246 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKR 305
           WK+KLV VP  VT   + +SL++N  K++++WQYVAN++ E L I   +T  N ++    
Sbjct: 245 WKLKLVPVPHTVTFFSVLRSLEENATKLIHRWQYVANKLPEDLFITAYITKINSSQEGIS 304

Query: 306 NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 365
              A F SL+LG  D+L+ +M    P LGL K +C E SW++  L  +G+++   L++LL
Sbjct: 305 TIQAEFPSLFLGGADRLLPLMQENFPELGLVKDDCTEMSWVEFVLYNSGYSSNSSLDVLL 364

Query: 366 SKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSK 425
           ++ T     ++K KSDYVK+P+ + AF+GIW R     +ET +L L PYGGKM  IS S 
Sbjct: 365 NR-TPQYITNFKAKSDYVKKPMPEIAFEGIWKRFLKVGIETPRLVLVPYGGKMDQISESS 424

Query: 426 TPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLD 485
            PF+HRA  LYKI Y + W EQG  A+ RH+ WIR  YS+  P+VS +PR AYINYRDLD
Sbjct: 425 IPFAHRAGNLYKIQYLLLWNEQGKEASMRHIAWIRRLYSYTAPYVSKNPREAYINYRDLD 484

Query: 486 IGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLT 534
           +G NN  G TSY++ASIWG KYF NNF RL+QVKT VDP NFFR+EQSIPPL+
Sbjct: 485 VGMNNIQGNTSYKQASIWGRKYFKNNFDRLIQVKTAVDPDNFFRNEQSIPPLS 534

BLAST of CSPI01G25200 vs. TAIR10
Match: AT5G44400.1 (AT5G44400.1 FAD-binding Berberine family protein)

HSP 1 Score: 484.2 bits (1245), Expect = 1.1e-136
Identity = 248/542 (45.76%), Postives = 347/542 (64.02%), Query Frame = 1

Query: 8   PLFLISFTIVSLFSTQWSTASSYKSKDFLQCLSIHSTHIPIYTPIN----------SSYS 67
           PLF I     SL++   +++ +     F+ C+   +TH+  Y P+           S +S
Sbjct: 8   PLFSILVLYFSLYTITPTSSLASLQDQFINCVQ-RNTHV--YFPLEKTFFAPTKNVSMFS 67

Query: 68  SILRSYAQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLS 127
            +L S AQN RF  + +  KP  I  P H SH+Q+++ICSK   + +R+RSGGHD EGLS
Sbjct: 68  QVLESTAQNLRFLKK-SMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLS 127

Query: 128 YIS--DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPT 187
           Y+S  D PF+++DL  ++ + ++  +N+AWVQSGAT+GELYYRIAEKS+   FPAG+C +
Sbjct: 128 YVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSS 187

Query: 188 VGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSF 247
           +GIGGH +GG YG +MRKYGL ADNV+DA +VDANG + DR AMGE  FWAIRGG GGSF
Sbjct: 188 LGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSF 247

Query: 248 GIVVGWKVKLVRVPPIVTTCRLDKSLDKNT-KKIVYQWQYVANRMEEKLLIGINL----T 307
           GI++ WK+KLV VP  VT   + K+L ++   KI+ +WQ VA+++ E+L I +      T
Sbjct: 248 GIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGT 307

Query: 308 GGNPTKGVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGF 367
           GGN T       T S+ +L+LG    L+ +M  + P LGLT  +C E SW++S    +GF
Sbjct: 308 GGNKT------VTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGF 367

Query: 368 TNGQPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYG 427
               P  +LL   +    +S+K KSD+VK PI +   +GI+ +L  +++    +   PYG
Sbjct: 368 PTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYG 427

Query: 428 GKMRNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPR 487
           G M  I  S+ PF HR   L+K+ Y   W +     + RH+NWIR+ YS+MTP+VS++PR
Sbjct: 428 GMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKRPS-RHINWIRDLYSYMTPYVSSNPR 487

Query: 488 AAYINYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIP 533
            AY+NYRDLD+G N K  KT  ++A +WG  YF NNF RL+ +K  VDP NFFRHEQSIP
Sbjct: 488 EAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 536

BLAST of CSPI01G25200 vs. TAIR10
Match: AT5G44440.1 (AT5G44440.1 FAD-binding Berberine family protein)

HSP 1 Score: 483.4 bits (1243), Expect = 1.8e-136
Identity = 250/530 (47.17%), Postives = 342/530 (64.53%), Query Frame = 1

Query: 10  FLISFTIVSLFSTQWSTASSYKSKDFLQCLSIH---STHIP--IYTPINSSYSSILRSYA 69
           FL +  + SL  +   +A     +DFL+CLS     +T  P  I+T  +SS+ SIL S  
Sbjct: 6   FLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSI 65

Query: 70  QNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS-DLP 129
           QNPRFS    T KP+ II P   S +Q+ + C++ HG+ +R RS GH  EGLSYI+ + P
Sbjct: 66  QNPRFSVS-ETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKP 125

Query: 130 FVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGHFS 189
           F V+DL NL+SI +D  N T WVQ+GAT GELYY I + +++LAFPAG+ PTVG+GG FS
Sbjct: 126 FAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFS 185

Query: 190 GGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGWKV 249
           GGGYG L+RKYGLAADN+IDA +VDA+G + DR+AMGE  FWAIRGGGG SFG+++ WKV
Sbjct: 186 GGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKV 245

Query: 250 KLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRNPT 309
           KLV VP  +T  ++ K+  K   +I+ +WQY A+++ + L I   L   N     K    
Sbjct: 246 KLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN-----KNAVH 305

Query: 310 ASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLSKP 369
           A F  LY+G  + L+ +M    P LGL K  C+E SWI+S L  A F  G+ L +L ++ 
Sbjct: 306 ALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLTNRE 365

Query: 370 TLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKTPF 429
             S  +S+K K D+V++PI + A + IW RL++ E    ++ L P+GGKM  ++  +TPF
Sbjct: 366 RTS--LSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPF 425

Query: 430 SHRAEFLYKISYTVGWAEQ---GSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLD 489
            HR   LY+I Y   W E+        +++L W+   Y FMTP+VS SPR AY+N++D+D
Sbjct: 426 PHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMD 485

Query: 490 IGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIP 531
           +G      KT YEE   WG+KYF NNF+RLV+VKT VDP++FF  EQSIP
Sbjct: 486 LGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of CSPI01G25200 vs. TAIR10
Match: AT5G44360.1 (AT5G44360.1 FAD-binding Berberine family protein)

HSP 1 Score: 481.1 bits (1237), Expect = 8.9e-136
Identity = 253/536 (47.20%), Postives = 353/536 (65.86%), Query Frame = 1

Query: 10  FLISFTIVSLFSTQWSTASSYKSKD-FLQCLSIHSTHIPI------YTPINSSYSSILRS 69
           F +S  +V L     S +SS  SKD FL C+S HS    I      + P +  Y+   +S
Sbjct: 7   FALSLFLVFLVKWVNSDSSSSPSKDQFLSCMSTHSDSSFINPKSFIHKPDSRVYTDFSQS 66

Query: 70  Y-AQNPRFST-QYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 129
             +QN RF T  + + KP++I+ P   + +Q +++CS+  G+++R +SGGHD EGLSY+S
Sbjct: 67  LISQNYRFLTLNFTSQKPILIVTPRTDTEIQRSLLCSRKLGVKVRTKSGGHDYEGLSYLS 126

Query: 130 -DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIG 189
              PF+++DL+N++SIE++  + TAWV +GATIGELYY+IA+ S+   FPAG CP+VG+G
Sbjct: 127 LHSPFIILDLVNVRSIEINLADETAWVGAGATIGELYYKIAKSSKIHGFPAGTCPSVGVG 186

Query: 190 GHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFD-REAMGEGLFWAIRGGGGGSFGIV 249
           GHFSGGG+G +MRK+GLAADNV+DA  VDANG +++ R  MGE LFWAIRGGG  SFG+V
Sbjct: 187 GHFSGGGFGAMMRKHGLAADNVVDARFVDANGRIYNSRREMGEDLFWAIRGGGAASFGVV 246

Query: 250 VGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIG--INLTGGNPTK 309
           + WKVKLVRVP  VT  R +  L +N  KIV++WQ +A  +++ L I   ++++GG    
Sbjct: 247 LSWKVKLVRVPEKVTCFRRNLPLTQNMTKIVHRWQQIAAELDDNLFIRVIVSISGG---- 306

Query: 310 GVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPL 369
               +   +F + YLG  DKL+ +MN   P LGLT  +C E +WI S ++   +  GQPL
Sbjct: 307 ----SVQTTFQANYLGGIDKLIPLMNQKFPELGLTFQDCSEMTWIDS-IMYFNWKKGQPL 366

Query: 370 EILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNI 429
           E LL +    N++ +K KSD+VK PI +   +GIW R    EVE+  + + P GGKM  I
Sbjct: 367 ETLLDRGQRYNDLYFKAKSDFVKNPIPEIGLEGIWTRF--HEVESPIMIMEPLGGKMYEI 426

Query: 430 SSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINY 489
             ++TPF HR   LY I Y V W  +  G  E+H+ W+R  Y +M  +VS SPR AY+NY
Sbjct: 427 GETETPFPHRRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASPRGAYLNY 486

Query: 490 RDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPL 533
           RDLD+G N     TS+E+A +WG +YFG+NFKRL  VK  +DP+NFFR+EQS+PPL
Sbjct: 487 RDLDLGMNRGV-NTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQSVPPL 530

BLAST of CSPI01G25200 vs. TAIR10
Match: AT1G30700.1 (AT1G30700.1 FAD-binding Berberine family protein)

HSP 1 Score: 480.7 bits (1236), Expect = 1.2e-135
Identity = 247/532 (46.43%), Postives = 352/532 (66.17%), Query Frame = 1

Query: 9   LFLISFTIVSLFSTQWSTASSYKSKDFLQCLSI-----HSTHIPIYTPINSSYSSILRSY 68
           L L+ F +V ++    S++SS  S+ F QCL+      H     I+   N SYSS+L++ 
Sbjct: 5   LILVLFFVVFIWQ---SSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQAN 64

Query: 69  AQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYI--SD 128
            +N RF+T  +T KP +II  +H SH+Q+ + C K H LQ++IRSGGHD +GLSY+  S 
Sbjct: 65  IRNLRFNTT-STPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSG 124

Query: 129 LPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGGH 188
            PF V+D+ NL+S++VD  + TAWVQ+GA +GE+YY I EKS+TLA+PAG+CPTVG+GGH
Sbjct: 125 KPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGH 184

Query: 189 FSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVGW 248
            SGGGYG +MRKYGL  DN IDA +VD NG + DR+ MGE L+WAI GGGGGS+G+V+ +
Sbjct: 185 ISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAY 244

Query: 249 KVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKRN 308
           K+ LV VP  VT  R+ ++L++N   I+++WQ VA ++ ++L I   +   N T   ++ 
Sbjct: 245 KINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKT 304

Query: 309 PTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILLS 368
              +F +++LG T  L++I+N   P LGL +++C ETSWIQS L       G    +LL 
Sbjct: 305 VRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQ 364

Query: 369 KPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSKT 428
           +    N +  K KSDYV++PIS+   + IW ++   E+ T  +   PYGG+M  ISS+ T
Sbjct: 365 RNQPVNYL--KRKSDYVREPISRTGLESIWKKMIELEIPT--MAFNPYGGEMGRISSTVT 424

Query: 429 PFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLDI 488
           PF +RA  L+KI Y   W ++     +R++   R+ Y FMTPFVS +PR ++ NYRD+D+
Sbjct: 425 PFPYRAGNLWKIQYGANWRDE--TLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDL 484

Query: 489 GTNNKYGK-TSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPL 533
           G N+  GK +SY E   +G KYF  NF+RLV++KT VD  NFFR+EQSIP L
Sbjct: 485 GINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of CSPI01G25200 vs. TAIR10
Match: AT4G20820.1 (AT4G20820.1 FAD-binding Berberine family protein)

HSP 1 Score: 477.6 bits (1228), Expect = 9.8e-135
Identity = 253/540 (46.85%), Postives = 349/540 (64.63%), Query Frame = 1

Query: 1   MKFFRLNPLFLISFTIVSLFSTQWSTASSYKSKD--FLQCLSI--HSTHIP---IYTPIN 60
           MKF       LI FT  +L  +     S+ +S    FLQCLS+  + ++I    I+TP +
Sbjct: 1   MKFQSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPND 60

Query: 61  SSYSSILRSYAQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDT 120
           +S+SS+L S  QN RFS   +  KP++I+ P   S +QS V C++  G+ IR RSGGHD 
Sbjct: 61  TSFSSVLASSIQNQRFSAP-DVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDY 120

Query: 121 EGLSYISDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVC 180
           EGLSY++  PFV++DL NL+SI VD  N + WVQ+GATIGELYY I +K+RTLAFPAGVC
Sbjct: 121 EGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVC 180

Query: 181 PTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGG 240
           PTVG+GGHFSGGGYG L+RK+GLAAD+VIDA +VDA G + +R  MGE  FWAIRGGGG 
Sbjct: 181 PTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGS 240

Query: 241 SFGIVVGWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGN 300
           SF +V+ WK+ L+ VP  VT   + K  +++  KI+++WQ+VA+++ + L I + L    
Sbjct: 241 SFCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML---- 300

Query: 301 PTKGVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNG 360
             +  K    ASF  LYLG    L+ ++N   P LGL + +C E SWI+S +  A     
Sbjct: 301 --QRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAEL-GE 360

Query: 361 QPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKM 420
           +P+ +L  +   S  +++K KSD+V++P+ + A   +W RL+  E E +QL   P+GGKM
Sbjct: 361 EPINVLTKRTRAS--LAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKM 420

Query: 421 RNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAY 480
             I+  +TPF HR   +Y+I Y   W        E+++ W+   Y  M+ FV+ SPR AY
Sbjct: 421 SEIADYETPFPHRKGNIYEIQYLNYWR---GDVKEKYMRWVERVYDDMSEFVAKSPRGAY 480

Query: 481 INYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLT 534
           IN RDLD+G      ++ YEE   WG+KYF NNF+RLV+VKT+VDPS+FF  EQSIPP T
Sbjct: 481 INLRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPPFT 527

BLAST of CSPI01G25200 vs. NCBI nr
Match: gi|449435918|ref|XP_004135741.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 1069.7 bits (2765), Expect = 1.7e-309
Identity = 526/535 (98.32%), Postives = 529/535 (98.88%), Query Frame = 1

Query: 1   MKFFRLNPLFLISFTIVSLFSTQWSTASSYKSKDFLQCLSIHSTHIPIYTPINSSYSSIL 60
           MKFF LNPLFLISFTIVSLFSTQWSTASSYK+KDFLQCLSIHSTHIPIYTPINSSYSSIL
Sbjct: 1   MKFFPLNPLFLISFTIVSLFSTQWSTASSYKNKDFLQCLSIHSTHIPIYTPINSSYSSIL 60

Query: 61  RSYAQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120
           RSYAQN RFSTQY TLKPLVIIKPSHVSHLQSTVICSKSH LQIRIRSGGHDTEGLSYIS
Sbjct: 61  RSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG 180
           DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG
Sbjct: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG 180

Query: 181 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVG 240
           HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGE LFWAIRGGGGGSFGIVVG
Sbjct: 181 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGGGGGSFGIVVG 240

Query: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKR 300
           WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKR
Sbjct: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKR 300

Query: 301 NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360
           NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL
Sbjct: 301 NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360

Query: 361 SKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSK 420
           SKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKM NISSSK
Sbjct: 361 SKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMSNISSSK 420

Query: 421 TPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLD 480
           TPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAY+NYRDLD
Sbjct: 421 TPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYVNYRDLD 480

Query: 481 IGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTLS 536
           IGTNNKYGKTSYEEASIWGLKYFGNNFK+LVQVKTTVDPSNFFRHEQSIPPLTLS
Sbjct: 481 IGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSIPPLTLS 535

BLAST of CSPI01G25200 vs. NCBI nr
Match: gi|659068699|ref|XP_008445749.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 1021.5 bits (2640), Expect = 5.2e-295
Identity = 497/535 (92.90%), Postives = 513/535 (95.89%), Query Frame = 1

Query: 1   MKFFRLNPLFLISFTIVSLFSTQWSTASSYKSKDFLQCLSIHSTHIPIYTPINSSYSSIL 60
           MK F LNPL   SFT+VSL STQWSTASSYK+KDFL+CLSIHST IPIYTPINSSY+S L
Sbjct: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60

Query: 61  RSYAQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120
           RSYAQNPRFSTQY TLKPL IIKPSHVSH+QSTVICSKSHGLQIRIRSGGHDTEGLSYIS
Sbjct: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG 180
           DLPF+VVDLINLKSIEVDTTNNT WVQSGATIGELYYRIAEKSRTLAFPAGVCPTVG+GG
Sbjct: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGVGG 180

Query: 181 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVG 240
            FSGGGYGWLMRKYGLAADNVIDAYLVDANG VFD EAMGEGLFWAIRGGGGGSFGIVVG
Sbjct: 181 QFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGGGGGSFGIVVG 240

Query: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTKGVKR 300
           WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVAN+MEE LLIGINLTGGNPTKGVKR
Sbjct: 241 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANKMEENLLIGINLTGGNPTKGVKR 300

Query: 301 NPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360
           NPTASFFSLYLGKTDKL+ IMNTT PNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL
Sbjct: 301 NPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAGFTNGQPLEILL 360

Query: 361 SKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNISSSK 420
           +KPTLSNNISYKIKSDYVKQPIS+HAFKGIWDRL+S EVETSQLFLFPYGGKM NISSSK
Sbjct: 361 TKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPYGGKMNNISSSK 420

Query: 421 TPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINYRDLD 480
           TPFSHRAEFLYKISYTVGWAEQG+GANERHLNWIRE YSFMTPFVSNSPRAAY+NYRDLD
Sbjct: 421 TPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSPRAAYVNYRDLD 480

Query: 481 IGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPLTLS 536
           IGTNNK GKTSYEEASIWG+KYFGNNFKRLVQVKT VDPSNFFRH+QSIPPLTLS
Sbjct: 481 IGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSIPPLTLS 535

BLAST of CSPI01G25200 vs. NCBI nr
Match: gi|659068690|ref|XP_008445695.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 678.7 bits (1750), Expect = 8.2e-192
Identity = 337/536 (62.87%), Postives = 408/536 (76.12%), Query Frame = 1

Query: 5   RLNPLFLISFTIVSLFSTQWSTASSYKSKDFLQCLSIHSTHI-----PIYTPINSSYSSI 64
           +L PLF +   +V   S  W +A  ++ ++FL CL  HS+        +YTPINSSYSS+
Sbjct: 6   QLTPLFHVLIIVVVSSSPLWVSAFPHEHQNFLHCLPEHSSKSYPISKVVYTPINSSYSSV 65

Query: 65  LRSYAQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYI 124
           L    +N RFS    T KPL+II PSHVSH+Q+ VICSKSHGLQIR RSGGHD EGLSY+
Sbjct: 66  LNFSIRNFRFSKP-ETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGLSYV 125

Query: 125 SDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIG 184
           +  PF++VDLIN++SI VD  NNTAWV+SGAT+GELYYRI EKSRTLAFPAG+CPTVG+G
Sbjct: 126 AYRPFIIVDLINIRSISVDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTVGVG 185

Query: 185 GHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVV 244
           GHFSGGGYG+L+RKYGLAADNVIDAYLVDANG   DRE+MGE LFWAIRGGGGGSFGIVV
Sbjct: 186 GHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMAHDRESMGEDLFWAIRGGGGGSFGIVV 245

Query: 245 GWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGN-PTKGV 304
            WKVKLV VP +VT C   K+L+ +  K+++QWQYVAN++ E + +GI LTGGN  T+G 
Sbjct: 246 AWKVKLVPVPALVTICSTIKNLEGDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTSTQGG 305

Query: 305 KRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAG--FTNGQPL 364
            + PTA+F+SL+LGK D+LV  ++TT P  GL K +C E  WI+STLI A   F   + L
Sbjct: 306 IKKPTATFYSLFLGKVDELVATLSTTFPEFGLIKKDCLEVRWIESTLIIASGVFQTVESL 365

Query: 365 EILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNI 424
           + LL++ T S   S KIKSDY+K+PI + A +GIW RLK+ ++E  QL   PYG +M  I
Sbjct: 366 KPLLNR-TPSTIDSTKIKSDYIKKPIPKAAIEGIWQRLKAGDIEAPQLVFVPYGARMSQI 425

Query: 425 SSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINY 484
           S S+TPFSHRA +LYKI Y VGW  Q   A  RH++W RE Y +MTPFVS SPR AY+NY
Sbjct: 426 SESETPFSHRAGYLYKIGYMVGWKGQNLKAKNRHISWTRELYEYMTPFVSKSPRTAYVNY 485

Query: 485 RDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPL 533
           RDLDIG+NNKYGKTSY++ASIWGLKYFGNNFKRLV VK  VDP +FFRHEQSIP L
Sbjct: 486 RDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKNKVDPHDFFRHEQSIPTL 539

BLAST of CSPI01G25200 vs. NCBI nr
Match: gi|778662000|ref|XP_011659198.1| (PREDICTED: cannabidiolic acid synthase-like 1 [Cucumis sativus])

HSP 1 Score: 676.0 bits (1743), Expect = 5.3e-191
Identity = 340/540 (62.96%), Postives = 413/540 (76.48%), Query Frame = 1

Query: 5   RLNPLFLISFTIVSLFSTQWSTASSYKSKDFLQCLSIHSTHI-----PIYTPINSSYSSI 64
           +L PLFL+  TIV   S  W +A  +K ++F+QCLS HS+        +YTPINSSYSS+
Sbjct: 6   QLIPLFLV-LTIVLSASPSWVSAFPHKHQNFVQCLSEHSSKSYPISKVVYTPINSSYSSV 65

Query: 65  LRSYAQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYI 124
           L    +N RFS    T KPL+II PSHVSH+Q+ VICSKSH LQIR RSGGHD EGLSY+
Sbjct: 66  LNFSIRNLRFSKP-ETPKPLLIITPSHVSHIQAAVICSKSHDLQIRTRSGGHDYEGLSYV 125

Query: 125 SDLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIG 184
           +  PF++VDLINL+SI +D  NNTAWV+SGAT+GELYYRI EKSRTLAFPAG+CPTVG+G
Sbjct: 126 AYHPFIIVDLINLRSISIDIVNNTAWVESGATLGELYYRIGEKSRTLAFPAGICPTVGVG 185

Query: 185 GHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVV 244
           GHFSGGGYG+L+RKYGLAADNVIDAYLVDANG V DRE+MGE LFWAIRGG GGSFGIVV
Sbjct: 186 GHFSGGGYGFLLRKYGLAADNVIDAYLVDANGMVHDRESMGEDLFWAIRGGSGGSFGIVV 245

Query: 245 GWKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPTK--G 304
            WKVKLV VP +VT C   K+L+ +  K+++QWQYVAN++ E + +GI LTGGN +   G
Sbjct: 246 AWKVKLVPVPAMVTICSTIKNLEDDAVKVIHQWQYVANKLHEDIFLGIVLTGGNTSTQGG 305

Query: 305 VK---RNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAG--FTN 364
           +K   +NP A+F+SL+LGK D+LV  ++T  P LGL K +C E  W++STLI A   F  
Sbjct: 306 IKNPIKNPIATFYSLFLGKVDELVATLSTKFPELGLIKQDCLEVRWVESTLIIASGVFQT 365

Query: 365 GQPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGK 424
            + LE LL++ T +   S KIKSDY+K+PI + A +GIW RLK++++E  Q    PYG +
Sbjct: 366 IESLEPLLNR-TPTTLDSTKIKSDYIKKPIPKAAIEGIWQRLKARDIEGPQFVFAPYGAR 425

Query: 425 MRNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAA 484
           M  IS S+TPFSHRA +LY+I Y VGW  Q   A  RH++WIRE Y +M PFVS SPRAA
Sbjct: 426 MSQISKSETPFSHRAGYLYQIGYMVGWKGQNLKAKNRHISWIRELYEYMAPFVSKSPRAA 485

Query: 485 YINYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPL 533
           Y NYRDLDIG+NNKYGKTSY++ASIWGLKYFGNNFKRLV VKT VDP +FFRHEQSIP L
Sbjct: 486 YTNYRDLDIGSNNKYGKTSYKQASIWGLKYFGNNFKRLVYVKTKVDPHDFFRHEQSIPTL 542

BLAST of CSPI01G25200 vs. NCBI nr
Match: gi|778661997|ref|XP_004151192.2| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 675.6 bits (1742), Expect = 7.0e-191
Identity = 340/536 (63.43%), Postives = 415/536 (77.43%), Query Frame = 1

Query: 6   LNPLFLISFTIVSLFSTQWSTASSYKSKDFLQCLSIHSTHI-----PIYTPINSSYSSIL 65
           L P  LI   + S  S+ W +AS +K  +FLQCLS HS+        ++TPINSSYSS+L
Sbjct: 7   LIPFSLILIVVSS--SSLWVSASPHKHHNFLQCLSEHSSKSYPISKVVHTPINSSYSSVL 66

Query: 66  RSYAQNPRFSTQYNTLKPLVIIKPSHVSHLQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 125
               +N RFS    T KPL+II PSHVSH+++ VICSKSHGLQIR RSGGHD EGLSY++
Sbjct: 67  NFSIRNLRFSKP-ETPKPLLIITPSHVSHIRAAVICSKSHGLQIRTRSGGHDYEGLSYVA 126

Query: 126 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIAEKSRTLAFPAGVCPTVGIGG 185
             PF+++DLINL+S+++D  +NTAWV+SG T+GELYYRI EKSRTLAFPAG+CPTVG+GG
Sbjct: 127 SHPFIILDLINLRSMKIDVESNTAWVESGTTLGELYYRIGEKSRTLAFPAGICPTVGVGG 186

Query: 186 HFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEGLFWAIRGGGGGSFGIVVG 245
           HFSGGGYG ++RK+GLAADNVIDAYLVDANG V DRE+MGE LFWAIRGGGGGSFGIVV 
Sbjct: 187 HFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVA 246

Query: 246 WKVKLVRVPPIVTTCRLDKSLDKNTKKIVYQWQYVANRMEEKLLIGINLTGGNPT---KG 305
           WK+KLV VP  VT C   ++L  +  K+V+QWQYVAN+++E L +GI LTGGN T   +G
Sbjct: 247 WKIKLVSVPATVTMCNTYRNLKGDGIKLVHQWQYVANKLDENLFLGIILTGGNITTTQEG 306

Query: 306 VKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAA-GFTNGQPL 365
           +  NP A+FFSL+LGK ++LV  ++TT P LGL K +C ETSWI+STLIA+ G    + L
Sbjct: 307 I-TNPIATFFSLFLGKVNELVATLSTTFPELGLIKQDCIETSWIESTLIASTGVQTVESL 366

Query: 366 EILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPYGGKMRNI 425
           E LL++ T SN  + KIKSDY+K+PIS    +GIW RLK+Q++ETSQL   PYGG+M  I
Sbjct: 367 EPLLNR-TPSNLENEKIKSDYIKEPISIATIEGIWQRLKAQDIETSQLIFIPYGGRMSQI 426

Query: 426 SSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSPRAAYINY 485
           S S+TPFSHR   LYKI Y +GW EQ   A ++H++WIRE Y +MTPFVS SPR+AY NY
Sbjct: 427 SESETPFSHRVGNLYKIGYILGWKEQSLKAKKKHISWIREIYEYMTPFVSKSPRSAYANY 486

Query: 486 RDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKRLVQVKTTVDPSNFFRHEQSIPPL 533
           RDLDIG N KYGKTS ++ASIWGLKYFGNNFKRLV VKT VDP +FFRHEQSIP L
Sbjct: 487 RDLDIGVNKKYGKTSVKQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537