CSPI01G25170 (gene) Wild cucumber (PI 183967)

BLAST of CSPI01G25170 vs. Swiss-Prot
Match: THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 6.1e-150
Identity = 266/543 (48.99%), Postives = 364/543 (67.03%), Query Frame = 1

Query: 17  CSSSSLW-------------IAASSSINHEEFIQCLFHHSTDTYFITKLVHTPINSSFSS 76
           CS+ S W             I  S +   E F++C   H  +     KLV+T  +  + S
Sbjct: 3   CSAFSFWFVCKIIFFFLSFHIQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMS 62

Query: 77  LLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYV 136
           +LN   +N RF +  TPKPL+I+TPSN SHIQA ++CSK  GLQIR RSGGHD EG+SY+
Sbjct: 63  ILNSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYI 122

Query: 137 AYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVG 196
           +   F+VVDL N+ S+ +DV   TAWVE+GATLGE+YY I +K+  L FP G CPTVGVG
Sbjct: 123 SQVPFVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVG 182

Query: 197 GHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 256
           GHFSGGGYG+L+R YG+AADN+IDA+LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI+ 
Sbjct: 183 GHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIA 242

Query: 257 AWKVKLVPVPATVTICSAKRSLE-EDAIKLIDQWQYVANKLEEDLFLAIFWNGGKVSAQE 316
           AWK+KLV VP+  TI S K+++E    +KL ++WQ +A K ++DL L   +    ++   
Sbjct: 243 AWKIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNH 302

Query: 317 G-GKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAA--SAANRFQI 376
           G  K      F ++F G V+ L+ ++NK+FPELG+ K +CKE SWI++    S    F  
Sbjct: 303 GKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNT 362

Query: 377 EDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 436
            +   E LL+R+     +F +K DYVK+P+ + A+  I ++L  +D+      + PYGG 
Sbjct: 363 ANFKKEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGI 422

Query: 437 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 496
           M +ISES IPFPHRAGI+Y++ Y   WE++  E  ++H+NW+R +Y++ TP+VS++PR A
Sbjct: 423 MEEISESAIPFPHRAGIMYELWYTASWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLA 482

Query: 497 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSNVDPYNFFWHEQSIPLS 543
           Y+NYRDLD+G  N     NY QA ++G KYFG NFNRLVKVK+ VDP NFF +EQSIP  
Sbjct: 483 YLNYRDLDLGKTNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 542

BLAST of CSPI01G25170 vs. Swiss-Prot
Match: CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa GN=CBDAS PE=1 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 3.9e-149
Identity = 264/543 (48.62%), Postives = 372/543 (68.51%), Query Frame = 1

Query: 17  CSSSSLW-------------IAASSSINHEEFIQCLFHHSTDTYFITKLVHTPINSSFSS 76
           CS+ S W             I  S +   E F++C   +  +     KLV+T  N  + S
Sbjct: 3   CSTFSFWFVCKIIFFFFSFNIQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMS 62

Query: 77  LLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYV 136
           +LN    N RF++  TPKPL+I+TPS++SHIQ  ++CSK  GLQIR RSGGHD EG+SY+
Sbjct: 63  VLNSTIHNLRFTSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYI 122

Query: 137 AYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVG 196
           +   F++VDL N++S+ +DV   TAWVE+GATLGE+YY + +K+  L   AG CPTV  G
Sbjct: 123 SQVPFVIVDLRNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAG 182

Query: 197 GHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 256
           GHF GGGYG L+R YG+AADN+IDA+LV+ +G+  DR+SMGEDLFWA+RGGG  SFGI+V
Sbjct: 183 GHFGGGGYGPLMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIV 242

Query: 257 AWKVKLVPVPATVTICSAKRSLE-EDAIKLIDQWQYVANKLEEDLFLAIFWNGGKVSAQE 316
           AWK++LV VP + T+ S K+ +E  + +KL+++WQ +A K ++DL L   +    ++  +
Sbjct: 243 AWKIRLVAVPKS-TMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQ 302

Query: 317 GGKINPIALFF-TLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAA--SAANRFQI 376
           G     I  +F ++FLG V+ L+ ++NK+FPELG+ K +C++ SWI++    S    +  
Sbjct: 303 GKNKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDT 362

Query: 377 EDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 436
           ++   E LL+R+   +G+FK+K DYVK+P+ ++    I ++L  +DI     A+ PYGG 
Sbjct: 363 DNFNKEILLDRSAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGI 422

Query: 437 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 496
           M++ISES IPFPHRAGILY++ YI  WE++  E  ++HLNWIR IY++MTP+VSK+PR A
Sbjct: 423 MDEISESAIPFPHRAGILYELWYICSWEKQ--EDNEKHLNWIRNIYNFMTPYVSKNPRLA 482

Query: 497 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSNVDPYNFFWHEQSIPLS 543
           Y+NYRDLDIG N+     NY QA ++G KYFG NF+RLVKVK+ VDP NFF +EQSIP  
Sbjct: 483 YLNYRDLDIGINDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 542

BLAST of CSPI01G25170 vs. Swiss-Prot
Match: CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.7e-147
Identity = 256/512 (50.00%), Postives = 356/512 (69.53%), Query Frame = 1

Query: 33  EEFIQCLFHHSTDTYFITKLVHTPINSSFSSLLNFPTRNSRFSTPNTPKPLLIITPSNIS 92
           E F++CL  +        KLV+T  +  + S+LN   +N RF++  TPKPL+I TP N+S
Sbjct: 32  ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNVS 91

Query: 93  HIQAAVVCSKSHGLQIRIRSGGHDFEGLSYVAYHQFIVVDLINLKSVTVDVEQSTAWVES 152
           HIQ  ++CSK  GLQIR RSGGHD EG+SY++   F++VDL N+ SV +DV   TAWVES
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVES 151

Query: 153 GATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVD 212
           GATLGE+YY I + +  L FPAG CPTVG GGHFSGGGYG+L+R YG+AADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSAKRSLE-EDAIK 272
            +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  TI S K+++E  + +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 273 LIDQWQYVANKLEEDLFLAIFWNGGKVSAQEGGKINPIALFF-TLFLGNVNELMAILNKT 332
           L+++WQ +A   E++L L   +    ++  +G     I  +F ++F G V+ L+ ++NK+
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELGLTKEECKEKSWIESAA--SAANRFQIEDQPLEALLNRTPITHGSFKLKSDYVKEP 392
           FPELG+ K +CK+ SWI++    S    +   +   E LL+R+     +F +K DYVK+P
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVKKP 391

Query: 393 MTKAAIQGIWKRLESQDIEGVTLAVIPYGGRMNQISESKIPFPHRAGILYQIGYILGWEE 452
           + + A+  I ++L  +D+        PYGG M++ISES IPFPHRAGI Y+I YI  WE+
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASWEK 451

Query: 453 KGVEAEKRHLNWIREIYSYMTPFVSKSPRAAYVNYRDLDIGSNNKYGKINYKQACVFGSK 512
           +  E  ++H+NWIR +Y++ TP+VS++PR AY+NYRDLD+G  N     NY QA ++G K
Sbjct: 452 Q--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEK 511

Query: 513 YFGNNFNRLVKVKSNVDPYNFFWHEQSIPLSP 541
           YFG NFNRLVKVK+ VDP NFF +EQSIP  P
Sbjct: 512 YFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLP 541

BLAST of CSPI01G25170 vs. Swiss-Prot
Match: CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 4.8e-147
Identity = 255/512 (49.80%), Postives = 356/512 (69.53%), Query Frame = 1

Query: 33  EEFIQCLFHHSTDTYFITKLVHTPINSSFSSLLNFPTRNSRFSTPNTPKPLLIITPSNIS 92
           E F++C   +        KLV+T  +  + S+LN   +N RF++  TPKPL+IITP N+S
Sbjct: 32  ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVS 91

Query: 93  HIQAAVVCSKSHGLQIRIRSGGHDFEGLSYVAYHQFIVVDLINLKSVTVDVEQSTAWVES 152
           HIQ  ++CSK  GLQIR RSGGHD EG+SY++   F++VDL N+ SV +DV   TAWVE+
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEA 151

Query: 153 GATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVD 212
           GATLGE+YY I + +  L FPAG CPTVG GGHFSGGGYG+L+R YG+AADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 213 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSAKRSLE-EDAIK 272
            +G+  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  TI S K+++E  + +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 273 LIDQWQYVANKLEEDLFLAIFWNGGKVSAQEGGKINPIALFF-TLFLGNVNELMAILNKT 332
           L+++WQ +A   E++L L   +    ++  +G     I  +F ++F G V+ L+ ++NK+
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 333 FPELGLTKEECKEKSWIESAA--SAANRFQIEDQPLEALLNRTPITHGSFKLKSDYVKEP 392
           FPELG+ K +CK+ SWI++    S    +       E LL+R+     +F +K DYVK+P
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKKP 391

Query: 393 MTKAAIQGIWKRLESQDIEGVTLAVIPYGGRMNQISESKIPFPHRAGILYQIGYILGWEE 452
           + + A+  I ++L  +D+        PYGG M++ISES IPFPHRAGI+Y+I YI  WE+
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWEK 451

Query: 453 KGVEAEKRHLNWIREIYSYMTPFVSKSPRAAYVNYRDLDIGSNNKYGKINYKQACVFGSK 512
           +  E  ++H+NWIR +Y++ TP+VS++PR AY+NYRDLD+G  N     NY QA ++G K
Sbjct: 452 Q--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEK 511

Query: 513 YFGNNFNRLVKVKSNVDPYNFFWHEQSIPLSP 541
           YFG NFNRLVKVK+ VDP NFF +EQSIP  P
Sbjct: 512 YFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLP 541

BLAST of CSPI01G25170 vs. Swiss-Prot
Match: THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.4e-146
Identity = 258/543 (47.51%), Postives = 363/543 (66.85%), Query Frame = 1

Query: 17  CSSSSLW-------------IAASSSINHEEFIQCLFHHSTDTYFITKLVHTPINSSFSS 76
           CS+ S W             I  S +   E F++C   +  +     K ++T  +  + S
Sbjct: 3   CSAFSFWFVCKIIFFFLSFNIQISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMS 62

Query: 77  LLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYV 136
           +LN   +N RF++  TPKPL+I+TPSN+SHIQA+++CSK  GLQIR RSGGHD EGLSY+
Sbjct: 63  VLNSTIQNLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYI 122

Query: 137 AYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVG 196
           +   F +VDL N+ +V VD+   TAWVE+GATLGE+YY I + +    FP G CPTVGVG
Sbjct: 123 SQVPFAIVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVG 182

Query: 197 GHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 256
           GHFSGGGYG+L+R YG+AADN+IDA+LV+ +G+  DR+SMGEDLFWAIRGGGG +FGI+ 
Sbjct: 183 GHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIA 242

Query: 257 AWKVKLVPVPATVTICSAKRSLE-EDAIKLIDQWQYVANKLEEDLFLAIFWNGGKVSAQE 316
           AWK+KLV VP+  TI S K+++E    +KL ++WQ +A K ++DL L   +    ++   
Sbjct: 243 AWKIKLVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNH 302

Query: 317 G-GKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAA--SAANRFQI 376
           G  K      F ++FLG V+ L+ ++NK+FPELG+ K +CKE SWI++    S    +  
Sbjct: 303 GKNKTTVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNT 362

Query: 377 EDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 436
            +   E LL+R+     +F +K DYVK+ + + A+  I ++L  +++      + PYGG 
Sbjct: 363 ANFKKEILLDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGI 422

Query: 437 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 496
           M++ISES IPFPHRAGI+Y++ Y   WE++  E  ++H+NW+R +Y++ TP+VS++PR A
Sbjct: 423 MDEISESAIPFPHRAGIMYELWYTATWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLA 482

Query: 497 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSNVDPYNFFWHEQSIPLS 543
           Y+NYRDLD+G  N     NY QA ++G KYFG NFNRLVKVK+  DP NFF +EQSIP  
Sbjct: 483 YLNYRDLDLGKTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 542

BLAST of CSPI01G25170 vs. TrEMBL
Match: B9NA97_POPTR (FAD-binding domain-containing family protein OS=Populus trichocarpa GN=POPTR_0001s43120g PE=4 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 2.8e-178
Identity = 305/531 (57.44%), Postives = 411/531 (77.40%), Query Frame = 1

Query: 9   LSLVFVVVCSSSSLWIAASSSINHEEFIQCLFHHSTDTYFITKLVHTPINSSFSSLLNFP 68
           LS +F ++ S S  W+  +S+  HE F+QCL   S +++ I+KL++TPINSS+SS+L F 
Sbjct: 2   LSFLFFLIFSFS--WV--TSAHTHESFLQCL--DSQNSHSISKLIYTPINSSYSSILQFS 61

Query: 69  TRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYVAYHQF 128
            +N RF+T +TPKPL+I+TP+NISHIQAA++CS+ HG+QIRIRSGGHD+EGLSYV+   F
Sbjct: 62  IQNLRFNTSSTPKPLVIVTPTNISHIQAAIICSQKHGMQIRIRSGGHDYEGLSYVSTFSF 121

Query: 129 IVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSG 188
           +V+DLINL+++ V+ E  TAWV+SG T+GE+YY+I +KSRTL FPA +CPTVGVGGHFSG
Sbjct: 122 VVIDLINLRTINVNAENKTAWVQSGVTIGEVYYRIAEKSRTLAFPASVCPTVGVGGHFSG 181

Query: 189 GGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVK 248
           GGYG L+RKYG+AAD++IDA LVD  G   DRESMGEDLFWAIRGGGG +FG+V+AWK+ 
Sbjct: 182 GGYGMLMRKYGLAADHIIDAQLVDVKGRILDRESMGEDLFWAIRGGGGNTFGVVIAWKIS 241

Query: 249 LVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAIFWNGGKVSAQEGGKINP 308
           LV  P TVT+ + +R+LE++A KL+ +WQ+VA+KL EDLF+ I  N  +V++ E GK N 
Sbjct: 242 LVSAPPTVTVFNVQRTLEQNASKLVHRWQFVADKLHEDLFIRIILN--RVNSTEEGKSNT 301

Query: 309 I--ALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIEDQPLEA 368
              A F +LFLG ++ L+ ++  +FPELGL KE+C E SWI+S     + F   +  L+ 
Sbjct: 302 TIQASFNSLFLGGIDRLLPLIQDSFPELGLVKEDCIEMSWIQSVL-YFDGFP-SNSSLDV 361

Query: 369 LLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRMNQISES 428
           LL+RTP T  +FK KSDYVKEP+ +  ++GIW+R   +DI    L   PYGG+M++ISES
Sbjct: 362 LLDRTPSTRRNFKAKSDYVKEPIPELGLEGIWERFFDKDINTPILIFSPYGGKMSEISES 421

Query: 429 KIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAYVNYRDL 488
            IPFPHRAG +Y+I +++ W+E+G+ A KRH++WIR +YSY+ P+VSK+PRAAYVNYRDL
Sbjct: 422 SIPFPHRAGNIYKIQHLIYWDEEGIVATKRHISWIRRLYSYLAPYVSKTPRAAYVNYRDL 481

Query: 489 DIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSNVDPYNFFWHEQSIP 538
           DIG NN  G  +Y+QA ++G KYF NNF+RLV+VK+ VDP NFF +EQSIP
Sbjct: 482 DIGINNHAGNTSYRQASIWGLKYFKNNFDRLVRVKTAVDPANFFRNEQSIP 522

BLAST of CSPI01G25170 vs. TrEMBL
Match: G8A1B3_MEDTR (Reticuline oxidase OS=Medicago truncatula GN=MTR_4g094488 PE=4 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 7.7e-176
Identity = 302/531 (56.87%), Postives = 402/531 (75.71%), Query Frame = 1

Query: 9   LSLVFVVVCSSSSLWIAASSSINHEEFIQCLFHHSTDTYFITKLVHTPINSSFSSLLNFP 68
           L++V + +  S + + A  +S + + F+QCL+ +S +   I+K+V+T  NSS+SS+L F 
Sbjct: 8   LTIVLIAIAFSFTSF-AIDTSPHEDNFLQCLYSYSHNITSISKVVYTKTNSSYSSILKFS 67

Query: 69  TRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYVAYHQF 128
            +N RF+T  TPKPL+IITP+ ISHIQ A++CS+ HG+QIRIRSGGHDFEGLS+V+   F
Sbjct: 68  IQNLRFATNETPKPLVIITPTQISHIQTAIICSQHHGMQIRIRSGGHDFEGLSFVSNVPF 127

Query: 129 IVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSG 188
           +++DL N + + VDVE  TAWV+SGATLGELYYKI +KS+TLGFP G+CPTVGVGGHFSG
Sbjct: 128 VIIDLTNFRGIDVDVENRTAWVQSGATLGELYYKIAQKSKTLGFPGGVCPTVGVGGHFSG 187

Query: 189 GGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVK 248
           GGYG+LLRKYG+AADNVIDA+++D  G F DRE+MGEDLFWAIRGGGG SFG++V+WK+K
Sbjct: 188 GGYGTLLRKYGLAADNVIDAHIIDVKGRFLDREAMGEDLFWAIRGGGGASFGVIVSWKIK 247

Query: 249 LVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAIFWNGGKVSAQEGG--KI 308
           LV VP+TVT+ +  R+LE++A KL+ +WQ+VA+KLEE+L + I      +++ + G  K 
Sbjct: 248 LVQVPSTVTVFTVPRTLEQNATKLVHKWQFVAHKLEENLAINIILQRLDLNSSKQGEPKS 307

Query: 309 NPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIEDQPLEA 368
             +ALF +LFLG+V+ L+ ++ + FPELGL +E+C E SWIES      RF  E +PLE 
Sbjct: 308 TVLALFQSLFLGSVDNLLPLMEEKFPELGLVREDCVEMSWIESVLYLF-RFP-EGEPLET 367

Query: 369 LLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRMNQISES 428
           LLNRT     + K KSD+VK P+ +  ++G+W   +    E V + + PYGG M++ISES
Sbjct: 368 LLNRTLAAKDNSKAKSDFVKIPIPETGLEGLWPLFDEDGAEDVLMVLFPYGGIMDKISES 427

Query: 429 KIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAYVNYRDL 488
           +IPFPHR G LY+I Y + W ++G E EK H+NWIR++YSYM PFVSKSPRAAY+NYRDL
Sbjct: 428 EIPFPHRYGTLYKIQYAVHWHQEGDEVEKLHINWIRKLYSYMEPFVSKSPRAAYINYRDL 487

Query: 489 DIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSNVDPYNFFWHEQSIP 538
           DIG NN  G  +YKQA ++G KYF NNF RL KVK+ VDP NFF +EQSIP
Sbjct: 488 DIGVNNINGYTSYKQASIWGVKYFKNNFKRLAKVKTKVDPLNFFRNEQSIP 535

BLAST of CSPI01G25170 vs. TrEMBL
Match: B9N5Q8_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s16580g PE=4 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 3.2e-174
Identity = 304/527 (57.69%), Postives = 387/527 (73.43%), Query Frame = 1

Query: 11  LVFVVVCSSSSLWIAASSSINHEEFIQCLFHHSTDTYFITKLVHTPINSSFSSLLNFPTR 70
           L F+     S LW  ++S+  HE+F+QCL  +S D+   +KLV+TP NSS+  +L    +
Sbjct: 11  LPFLCFLLFSFLW--SASAHTHEDFLQCLSLYSEDSNSFSKLVYTPNNSSYLHVLQGSIQ 70

Query: 71  NSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYVAYHQFIV 130
           NSRF+T  TPKP +I+TP N+SHIQA + CS+ HG+ +RIRSGGHD+EGLSYV+   F++
Sbjct: 71  NSRFNTTATPKPPVIVTPMNVSHIQATIFCSQKHGMNVRIRSGGHDYEGLSYVSVLPFVI 130

Query: 131 VDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGG 190
           +DLINL+++ VDVE STAWV++GATLGELYY I +KS TL FPAG CPTVG GGH SGGG
Sbjct: 131 IDLINLRAINVDVENSTAWVQAGATLGELYYSIAEKSGTLAFPAGACPTVGAGGHLSGGG 190

Query: 191 YGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLV 250
           YG L+RKYG+AADN+IDA L+DA G   DR SMGEDLFWAIRGGGG +FG+VVAWK+KLV
Sbjct: 191 YGGLMRKYGLAADNIIDAQLIDAKGRILDRASMGEDLFWAIRGGGGNTFGVVVAWKLKLV 250

Query: 251 PVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAIFWNGGKVSAQEGGKINPIA 310
           PVP TVT  S  RSLEE+A KLI +WQYVANKL EDLF+  +    K+++ + G     A
Sbjct: 251 PVPHTVTFFSVLRSLEENATKLIHRWQYVANKLPEDLFITAYIT--KINSSQEGISTIQA 310

Query: 311 LFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIEDQPLEALLNR 370
            F +LFLG  + L+ ++ + FPELGL K++C E SW+E      N     +  L+ LLNR
Sbjct: 311 EFPSLFLGGADRLLPLMQENFPELGLVKDDCTEMSWVEFV--LYNSGYSSNSSLDVLLNR 370

Query: 371 TPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRMNQISESKIPF 430
           TP    +FK KSDYVK+PM + A +GIWKR     IE   L ++PYGG+M+QISES IPF
Sbjct: 371 TPQYITNFKAKSDYVKKPMPEIAFEGIWKRFLKVGIETPRLVLVPYGGKMDQISESSIPF 430

Query: 431 PHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAYVNYRDLDIGS 490
            HRAG LY+I Y+L W E+G EA  RH+ WIR +YSY  P+VSK+PR AY+NYRDLD+G 
Sbjct: 431 AHRAGNLYKIQYLLLWNEQGKEASMRHIAWIRRLYSYTAPYVSKNPREAYINYRDLDVGM 490

Query: 491 NNKYGKINYKQACVFGSKYFGNNFNRLVKVKSNVDPYNFFWHEQSIP 538
           NN  G  +YKQA ++G KYF NNF+RL++VK+ VDP NFF +EQSIP
Sbjct: 491 NNIQGNTSYKQASIWGRKYFKNNFDRLIQVKTAVDPDNFFRNEQSIP 531

BLAST of CSPI01G25170 vs. TrEMBL
Match: B9GGI2_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s46620g PE=4 SV=2)

HSP 1 Score: 618.2 bits (1593), Expect = 9.4e-174
Identity = 305/535 (57.01%), Postives = 402/535 (75.14%), Query Frame = 1

Query: 4   SPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLFHHSTDTYFITKLVHTPINSSFSS 63
           S + P   +F+   S    W+A++    HE+F+QCL + +++   I+K+++TPINSS+SS
Sbjct: 6   SSMFPFLFLFLFSFS----WVASAD--RHEDFLQCLHYQNSNA--ISKVIYTPINSSYSS 65

Query: 64  LLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYV 123
           +L F  RN RF+T  TPKPL+I+TP N++HIQAA+ CS+ HGLQIR+RSGGHD+EGLSYV
Sbjct: 66  VLQFSIRNGRFNTSATPKPLVIVTPLNVAHIQAAITCSQKHGLQIRVRSGGHDYEGLSYV 125

Query: 124 AYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVG 183
           A   F+VVDLIN+++VTVDV   TAWV+ GATLGE+YY+I +KSRTL FPAG+CPTVGVG
Sbjct: 126 AVIPFVVVDLINMRTVTVDVANKTAWVQGGATLGEVYYRIAEKSRTLAFPAGVCPTVGVG 185

Query: 184 GHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 243
           GH SGGG G ++RKYG+A D++IDA L+D  G   DR SMGEDLFWAIRGGGG +FG+VV
Sbjct: 186 GHISGGGTGMIMRKYGLAVDHIIDAQLIDVKGRILDRASMGEDLFWAIRGGGGNTFGVVV 245

Query: 244 AWKVKLVPVPATVTICSAKRSL-EEDAIKLIDQWQYVANKLEEDLFLAIFWNGGKVSAQE 303
           AWK++LVP+PA+VT+ +  R+L +EDA KLI QWQY   K +EDLF  IF      S QE
Sbjct: 246 AWKLELVPIPASVTVFNVTRTLMKEDATKLIHQWQYAIEKFDEDLFSRIFIWRSANSTQE 305

Query: 304 GGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIEDQ 363
            GK    A F +LFLG V+ L++ + ++FPELGL +E+C E SWIES    A +F   + 
Sbjct: 306 -GKPMIQAAFTSLFLGGVDRLLSYMQESFPELGLVREDCIEMSWIESTVYFA-QFP-RNT 365

Query: 364 PLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRMNQ 423
            LE LLNR+P +   FK K+D+VKEP+ K A++GIW+RL+  D E   L    YGG+MN+
Sbjct: 366 SLEVLLNRSPRSTIFFKGKTDFVKEPIPKTALEGIWERLDQVDAESAELQFTAYGGKMNE 425

Query: 424 ISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAYVN 483
           I+ES  PFPHRAG LYQI Y + W+E+G+EA  ++++WIR +YSYM P+VSK+PR AYVN
Sbjct: 426 IAESSTPFPHRAGTLYQIHYAISWDEEGIEAYAKYISWIRRLYSYMAPYVSKNPRQAYVN 485

Query: 484 YRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSNVDPYNFFWHEQSIP 538
           YRDLD+G NN  G  +Y+QA ++G+KYF NNF+RLV+VK+ VDP NFF +EQSIP
Sbjct: 486 YRDLDLGVNN-LGNTSYRQASIWGTKYFKNNFDRLVRVKTAVDPANFFRNEQSIP 528

BLAST of CSPI01G25170 vs. TrEMBL
Match: A0A059CEM7_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D01004 PE=4 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 6.1e-173
Identity = 293/505 (58.02%), Postives = 381/505 (75.45%), Query Frame = 1

Query: 33  EEFIQCLFHHSTDTYFITKLVHTPINSSFSSLLNFPTRNSRFSTPNTPKPLLIITPSNIS 92
           E F QCL  HS +T  I+KLV+TPINSS+SS+L+   +N RF TP+ PKPLLI+TP ++S
Sbjct: 31  ENFQQCLSLHSENTTAISKLVYTPINSSYSSVLDITIQNLRFLTPSAPKPLLIVTPLHVS 90

Query: 93  HIQAAVVCSKSHGLQIRIRSGGHDFEGLSYVAYHQFIVVDLINLKSVTVDVEQSTAWVES 152
           H+QAA+ CS+ +GLQ+RIRSGGHD+EGLSY++   F+++D++NL+S+ VDVE  TAWV+S
Sbjct: 91  HVQAAITCSRRYGLQMRIRSGGHDYEGLSYLSKVPFVIMDMVNLRSIAVDVEDETAWVQS 150

Query: 153 GATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVD 212
           GAT+GELYY+I +KS+TLGFPAG+CPTVGVGGHFSGGGYG++LRKYG+A DNVIDA LV+
Sbjct: 151 GATVGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGTMLRKYGLAVDNVIDAQLVN 210

Query: 213 ANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSAKRSLEEDAIKL 272
           A G   DRESMGEDLFWAIRGGGGGSFG+VV+WKVKLVPVP+TVT+ +  R+LE+ A KL
Sbjct: 211 AQGRLLDRESMGEDLFWAIRGGGGGSFGVVVSWKVKLVPVPSTVTVFTLLRTLEQHATKL 270

Query: 273 IDQWQYVANKLEEDLFLAIFWNGGKVSAQEGGKINPIALFFTLFLGNVNELMAILNKTFP 332
           + QWQ + N+L  DLF+ +       ++   G     A F TL+LG V+ L+ +   +FP
Sbjct: 271 VHQWQLIGNELPNDLFMRVILQSS--NSGPNGTRTMEASFNTLYLGGVDALLEVTRSSFP 330

Query: 333 ELGLTKEECKEKSWIESAASAANRFQIEDQPLEALLNRTPITHGSFKLKSDYVKEPMTKA 392
           EL +T+++C E SWIES    A       +PLE LLNR+  T   FK KSDYVKEP+ K+
Sbjct: 331 ELNVTRQDCTEMSWIESILYFAG---FSGEPLEVLLNRSSNTKLYFKHKSDYVKEPIPKS 390

Query: 393 AIQGIWKRLESQDIEGVTLAVIPYGGRMNQISESKIPFPHRAGILYQIGYILGWEEKGVE 452
            ++G+W+R   +D+    +    YGGRM++ISES +PFPHRAG+LY+I ++  W + G E
Sbjct: 391 GLEGMWERFFEEDVGVAQMIWNHYGGRMSEISESALPFPHRAGVLYKIQHLTSWTDGGDE 450

Query: 453 AEKRHLNWIREIYSYMTPFVSKSPRAAYVNYRDLDIGSNNKYGKINYKQACVFGSKYFGN 512
              +H++WIR  YSYM P+VSKSPR AY+NYRDLDIG+NN  G   YKQA  +G KYF N
Sbjct: 451 VASKHVSWIRSFYSYMAPYVSKSPREAYINYRDLDIGTNNVKGDTGYKQASAWGKKYFKN 510

Query: 513 NFNRLVKVKSNVDPYNFFWHEQSIP 538
           NF+RLV+VK+ VD  NFF HEQSIP
Sbjct: 511 NFDRLVRVKTAVDRGNFFRHEQSIP 530

BLAST of CSPI01G25170 vs. TAIR10
Match: AT4G20820.1 (AT4G20820.1 FAD-binding Berberine family protein)

HSP 1 Score: 517.7 bits (1332), Expect = 8.7e-147
Identity = 247/507 (48.72%), Postives = 357/507 (70.41%), Query Frame = 1

Query: 31  NHEEFIQCLFHHSTDTYFITKLVHTPINSSFSSLLNFPTRNSRFSTPNTPKPLLIITPSN 90
           N   F+QCL     D+  ++K++HTP ++SFSS+L    +N RFS P+ PKP+LI+TP  
Sbjct: 33  NQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQ 92

Query: 91  ISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYVAYHQFIVVDLINLKSVTVDVEQSTAWV 150
            S +Q+AV C++  G+ IR RSGGHD+EGLSYV +  F+++DL NL+S+TVDV+  + WV
Sbjct: 93  PSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWV 152

Query: 151 ESGATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYL 210
           ++GAT+GELYY+IGKK+RTL FPAG+CPTVGVGGHFSGGGYG+LLRK+G+AAD+VIDA +
Sbjct: 153 QTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARV 212

Query: 211 VDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSAKRSLEEDAI 270
           VDA G   +R  MGED FWAIRGGGG SF +V++WK+ L+ VP+TVT+ +  +  E+ A+
Sbjct: 213 VDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQSAL 272

Query: 271 KLIDQWQYVANKLEEDLFLAIFWNGGKVSAQEGGKINPIALFFTLFLGNVNELMAILNKT 330
           K+I +WQ+VA+K+ +DLF+ +     K   +        A F  L+LG+V  L+ ++NK 
Sbjct: 273 KIIHRWQFVADKVSDDLFIRVMLQRYKNMVR--------ASFPGLYLGSVKNLLKMVNKE 332

Query: 331 FPELGLTKEECKEKSWIESAASAANRFQIEDQPLEALLNRTPITHGSFKLKSDYVKEPMT 390
           FPELGL +++C E SWIES    A   ++ ++P+  L  RT  +  +FK KSD+V+EPM 
Sbjct: 333 FPELGLEEDDCTEMSWIESVIWFA---ELGEEPINVLTKRTRASL-AFKAKSDFVQEPMP 392

Query: 391 KAAIQGIWKRLESQDIEGVTLAVIPYGGRMNQISESKIPFPHRAGILYQIGYILGWEEKG 450
           K AI  +W+RL+  + E   L   P+GG+M++I++ + PFPHR G +Y+I Y+  W    
Sbjct: 393 KTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYWRG-- 452

Query: 451 VEAEKRHLNWIREIYSYMTPFVSKSPRAAYVNYRDLDIGSNNKYGKINYKQACVFGSKYF 510
            + +++++ W+  +Y  M+ FV+KSPR AY+N RDLD+G      +  Y++   +G KYF
Sbjct: 453 -DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGKSWGVKYF 512

Query: 511 GNNFNRLVKVKSNVDPYNFFWHEQSIP 538
            NNF RLV+VK++VDP +FF  EQSIP
Sbjct: 513 KNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of CSPI01G25170 vs. TAIR10
Match: AT5G44440.1 (AT5G44440.1 FAD-binding Berberine family protein)

HSP 1 Score: 516.9 bits (1330), Expect = 1.5e-146
Identity = 255/534 (47.75%), Postives = 372/534 (69.66%), Query Frame = 1

Query: 10  SLVFVVVCSSSSLW-IAASSSINHEEFIQCLFHHSTDTYFITKLVHTPINSSFSSLLNFP 69
           S +F ++  S ++  + ++   NHE+F++CL +   D     K++HT  +SSF S+L+  
Sbjct: 5   SFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSS 64

Query: 70  TRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYVAYHQ- 129
            +N RFS   TPKP+ IITP   S +Q  + C++ HG+ +R RS GH +EGLSY+AY++ 
Sbjct: 65  IQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKP 124

Query: 130 FIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVGGHFS 189
           F V+DL NL+S+++DV+  T WV++GAT GELYY+IGK +++L FPAGI PTVGVGG FS
Sbjct: 125 FAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFS 184

Query: 190 GGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 249
           GGGYG+LLRKYG+AADN+IDA +VDA+G   DR++MGED FWAIRGGGG SFG++++WKV
Sbjct: 185 GGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKV 244

Query: 250 KLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAIFWNGGKVSAQEGGKIN 309
           KLV VP+T+T+   +++ +++A+++I +WQY A+K+ +DLF+       + + +   K  
Sbjct: 245 KLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFI-------RTTLERSNKNA 304

Query: 310 PIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIEDQPLEAL 369
             ALF  L++G VN L+A++ + FPELGL KE C+E SWIES    A+    + + L  L
Sbjct: 305 VHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD--FPKGESLGVL 364

Query: 370 LNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRMNQISESK 429
            NR   T  SFK K D+V+EP+ +AAIQ IW+RLE+ +     + + P+GG+M++++E +
Sbjct: 365 TNRER-TSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYE 424

Query: 430 IPFPHRAGILYQIGYILGW---EEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAYVNYR 489
            PFPHR G LY+I Y+  W   E+K      ++L W+  +Y +MTP+VSKSPR AYVN++
Sbjct: 425 TPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFK 484

Query: 490 DLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSNVDPYNFFWHEQSIPL 539
           D+D+G      K  Y++   +G KYF NNF RLV+VK+ VDP +FF  EQSIPL
Sbjct: 485 DMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPL 528

BLAST of CSPI01G25170 vs. TAIR10
Match: AT5G44410.1 (AT5G44410.1 FAD-binding Berberine family protein)

HSP 1 Score: 507.7 bits (1306), Expect = 9.0e-144
Identity = 252/539 (46.75%), Postives = 373/539 (69.20%), Query Frame = 1

Query: 6   LIPLSLVFVVVCSSSSLWIAASSS-INHEEFIQCLFHHSTDTYFITKLVHTPINSSFSSL 65
           L+ L + F+++  S S + + S+   NHE F++CL H   +    ++++HT  + S+ S+
Sbjct: 7   LLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINEDD--SRIIHTSKDPSYFSI 66

Query: 66  LNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYVA 125
           LN   +N RF    TPKP+ IITP   + +Q+ + C++ HG+ IR RSGGHD+EGLSY+A
Sbjct: 67  LNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMA 126

Query: 126 YHQ-FIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVG 185
             + F+V+DL NL+S+T+DV+  T WV+SGAT+GELYY+IGK S++L FPAG+ PTVG+G
Sbjct: 127 KSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIG 186

Query: 186 GHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 245
           G F GGGYG+L+RKYG++ADNVIDA++VDANG F DR+ MGED FWAIRGGGG SF +V+
Sbjct: 187 GQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVL 246

Query: 246 AWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAIFWNGGKVSAQEG 305
           +WK++L+ VP+ VT+    ++ E++A+ +I++WQY+A+K+  DLF         + A   
Sbjct: 247 SWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLF---------IRAMLQ 306

Query: 306 GKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIEDQP 365
            +    A F  L+LG V++L+A++   FPELGL    C+E SWIES       + I+ + 
Sbjct: 307 KETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVL-----WFIKGES 366

Query: 366 LEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRMNQI 425
           +E L  R   T  SFK K D+++EP+ K AIQ +W+R E+ +     + + P+GG+M++I
Sbjct: 367 MEILAKRKR-TSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEI 426

Query: 426 SESKIPFPHRAGILYQIGYILGW---EEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY 485
           ++++IPFPHR G LY+I Y+  W   E+K     +++L W+  +Y +MTP+VSKSPR AY
Sbjct: 427 ADNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAY 486

Query: 486 VNYRDLDIGSNNKYG-KINYKQACVFGSKYFGNNFNRLVKVKSNVDPYNFFWHEQSIPL 539
           VN+RD+D+G       K  Y++A V+G KYF NNF+RLV+VK+NVDP +FF  EQSIP+
Sbjct: 487 VNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPI 528

BLAST of CSPI01G25170 vs. TAIR10
Match: AT5G44400.1 (AT5G44400.1 FAD-binding Berberine family protein)

HSP 1 Score: 506.1 bits (1302), Expect = 2.6e-143
Identity = 258/543 (47.51%), Postives = 367/543 (67.59%), Query Frame = 1

Query: 1   MKLSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLFHHSTDTYF-ITKLVHTPIN- 60
           M +S  +PL  + V+  S  ++   +S +   ++FI C+   +T  YF + K    P   
Sbjct: 1   MGISKPLPLFSILVLYFSLYTITPTSSLASLQDQFINCV-QRNTHVYFPLEKTFFAPTKN 60

Query: 61  -SSFSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDF 120
            S FS +L    +N RF   + PKP  I +P + SH+QA+++CSK   + +R+RSGGHD+
Sbjct: 61  VSMFSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDY 120

Query: 121 EGLSYVAY--HQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAG 180
           EGLSYV+     FI++DL  ++ V ++++ ++AWV+SGAT+GELYY+I +KS+  GFPAG
Sbjct: 121 EGLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAG 180

Query: 181 ICPTVGVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGG 240
           +C ++G+GGH +GG YGS++RKYG+ ADNV+DA +VDANG+  DR +MGED FWAIRGG 
Sbjct: 181 LCSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGA 240

Query: 241 GGSFGIVVAWKVKLVPVPATVTICSAKRSLEED-AIKLIDQWQYVANKLEEDLFLAIFWN 300
           GGSFGI++AWK+KLVPVP TVT+ +  ++L++D   K+I +WQ VA+KL E+LF+ + +N
Sbjct: 241 GGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFN 300

Query: 301 GGKVSAQEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAA 360
                A  GG       +  LFLG    LM ++ K+FPELGLT ++C E SW+ES A  +
Sbjct: 301 ----VAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYIS 360

Query: 361 NRFQIEDQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVI 420
             F         L  ++P    SFK KSD+VK P+ ++ +QGI+K+L  +DI    +   
Sbjct: 361 G-FPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIP--LMIWN 420

Query: 421 PYGGRMNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSK 480
           PYGG M +I ES+IPFPHR G+L+++ Y+  W +   +   RH+NWIR++YSYMTP+VS 
Sbjct: 421 PYGGMMAKIPESQIPFPHRKGVLFKVQYVTSWLDSD-KRPSRHINWIRDLYSYMTPYVSS 480

Query: 481 SPRAAYVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSNVDPYNFFWHEQ 538
           +PR AYVNYRDLD+G N K  K   KQA V+G+ YF NNFNRL+ +K+ VDP NFF HEQ
Sbjct: 481 NPREAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQ 534

BLAST of CSPI01G25170 vs. TAIR10
Match: AT1G30700.1 (AT1G30700.1 FAD-binding Berberine family protein)

HSP 1 Score: 499.2 bits (1284), Expect = 3.2e-141
Identity = 258/537 (48.04%), Postives = 360/537 (67.04%), Query Frame = 1

Query: 9   LSLVFVVVCSSSSLWIAASSSINHEEFIQCLFHHSTDTYFITKLVHTPINSSFSSLLNFP 68
           L L FVV      +W ++SSS N E F QCL  +S   + I+  +    N S+SS+L   
Sbjct: 7   LVLFFVVF-----IWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQAN 66

Query: 69  TRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYVAYH-- 128
            RN RF+T +TPKP LII  ++ SH+QAA+ C K H LQ++IRSGGHD++GLSYV Y   
Sbjct: 67  IRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGK 126

Query: 129 QFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVGGHF 188
            F V+D+ NL+SV VDV   TAWV++GA LGE+YY I +KS+TL +PAGICPTVGVGGH 
Sbjct: 127 PFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHI 186

Query: 189 SGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVVAWK 248
           SGGGYG+++RKYG+  DN IDA +VD NG+  DR+ MGEDL+WAI GGGGGS+G+V+A+K
Sbjct: 187 SGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYK 246

Query: 249 VKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAIFWN--GGKVSAQEGG 308
           + LV VP  VT+    R+LE++A  +I +WQ VA KL ++LF+    +   G VS+Q+  
Sbjct: 247 INLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTV 306

Query: 309 KINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIEDQPL 368
           +      F  +FLG+   L++ILN+ FPELGL + +C E SWI+S     N  Q+     
Sbjct: 307 RTT----FIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTN-IQVGSSET 366

Query: 369 EALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRMNQIS 428
             L    P+ +   K KSDYV+EP+++  ++ IWK++   ++E  T+A  PYGG M +IS
Sbjct: 367 LLLQRNQPVNY--LKRKSDYVREPISRTGLESIWKKM--IELEIPTMAFNPYGGEMGRIS 426

Query: 429 ESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAYVNYR 488
            +  PFP+RAG L++I Y   W ++ +    R++   R++Y +MTPFVSK+PR ++ NYR
Sbjct: 427 STVTPFPYRAGNLWKIQYGANWRDETL--TDRYMELTRKLYQFMTPFVSKNPRQSFFNYR 486

Query: 489 DLDIGSNNKYGKI-NYKQACVFGSKYFGNNFNRLVKVKSNVDPYNFFWHEQSIPLSP 541
           D+D+G N+  GKI +Y +   +G KYF  NF RLVK+K+ VD  NFF +EQSIP+ P
Sbjct: 487 DVDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVLP 527

BLAST of CSPI01G25170 vs. NCBI nr
Match: gi|778662006|ref|XP_011659211.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 535/544 (98.35%), Postives = 539/544 (99.08%), Query Frame = 1

Query: 1   MKLSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLFHHSTDTYFITKLVHTPINSS 60
           MK SPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCL HHS DTY ITKLVHTPINSS
Sbjct: 1   MKFSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLLHHSPDTYLITKLVHTPINSS 60

Query: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120
           FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL
Sbjct: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120

Query: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAIFWNGGKVSA 300
           IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEE+LFLAIFWNGGKVSA
Sbjct: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEELFLAIFWNGGKVSA 300

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           QEGGKINPIALFFTLFLGNVNELMAIL+KTFPELGLTKEECKEKSWIESAASAANRFQIE
Sbjct: 301 QEGGKINPIALFFTLFLGNVNELMAILSKTFPELGLTKEECKEKSWIESAASAANRFQIE 360

Query: 361 DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRM 420
           DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRM
Sbjct: 361 DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRM 420

Query: 421 NQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY 480
           NQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY
Sbjct: 421 NQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY 480

Query: 481 VNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSNVDPYNFFWHEQSIPLSP 540
           VNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKS+VDPYNFFWHEQSIPLS 
Sbjct: 481 VNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSDVDPYNFFWHEQSIPLSS 540

Query: 541 NHMV 545
           +HMV
Sbjct: 541 DHMV 544

BLAST of CSPI01G25170 vs. NCBI nr
Match: gi|659068694|ref|XP_008445721.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 1023.8 bits (2646), Expect = 1.1e-295
Identity = 500/542 (92.25%), Postives = 520/542 (95.94%), Query Frame = 1

Query: 1   MKLSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLFHHSTDTYFITKLVHTPINSS 60
           M LSPL+PLSLVF+VVCSSSSLW A SSSI HEEF+QCL HHS DT  I KLVHTPINSS
Sbjct: 14  MMLSPLVPLSLVFIVVCSSSSLWAATSSSIKHEEFLQCLLHHSPDTSSINKLVHTPINSS 73

Query: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120
           FS+L NFPTRNSRFSTPNTPKPLLIITPSNISHIQAAV+CSKSHGLQIRIRSGGHDFEGL
Sbjct: 74  FSTLFNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVICSKSHGLQIRIRSGGHDFEGL 133

Query: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYVAYH+FIVVDLINL+SVTVDVEQSTAWV+SGATLGELYYKIGKKSRTLGFPAGICPTV
Sbjct: 134 SYVAYHRFIVVDLINLRSVTVDVEQSTAWVQSGATLGELYYKIGKKSRTLGFPAGICPTV 193

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG
Sbjct: 194 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 253

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAIFWNGGKVSA 300
           +VVAWKVKLVPVPA+VTICSAKRS+EE+AIKLID+WQYVANKLEEDLFLAI WNGGKVSA
Sbjct: 254 VVVAWKVKLVPVPASVTICSAKRSVEEEAIKLIDRWQYVANKLEEDLFLAILWNGGKVSA 313

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q GGKINPIALFF LFLGNVNELMAILNKTFPELG TKEECKEKSWIESAASAANRFQIE
Sbjct: 314 QGGGKINPIALFFPLFLGNVNELMAILNKTFPELGFTKEECKEKSWIESAASAANRFQIE 373

Query: 361 DQPLEALLNRTPITHG-SFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGR 420
           DQPLEALLNRTPI HG SFKLKSDYVKEPMTK AI+GIWKRLESQDIEGVTLA+IPYGGR
Sbjct: 374 DQPLEALLNRTPINHGSSFKLKSDYVKEPMTKTAIEGIWKRLESQDIEGVTLALIPYGGR 433

Query: 421 MNQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAA 480
           M++ISES IPF HRAGILYQIGYILGW+EKGVEAE+RHLNWIREIYSYMTPFVSKSPR A
Sbjct: 434 MSRISESNIPFSHRAGILYQIGYILGWDEKGVEAEERHLNWIREIYSYMTPFVSKSPRTA 493

Query: 481 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSNVDPYNFFWHEQSIPLS 540
           YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVK+ VDPYNFFWHEQSIPLS
Sbjct: 494 YVNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKTKVDPYNFFWHEQSIPLS 553

Query: 541 PN 542
            N
Sbjct: 554 SN 555

BLAST of CSPI01G25170 vs. NCBI nr
Match: gi|659068692|ref|XP_008445709.1| (PREDICTED: cannabidiolic acid synthase-like [Cucumis melo])

HSP 1 Score: 873.2 bits (2255), Expect = 2.3e-250
Identity = 421/538 (78.25%), Postives = 479/538 (89.03%), Query Frame = 1

Query: 1   MKLSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLFHHSTDTYFITKLVHTPINSS 60
           MK S L+PLSL F+VVCSSSS+W +ASS +NHEEF+ CL HHS D+  ITKLV+TPIN+S
Sbjct: 1   MKFSLLLPLSLAFIVVCSSSSVWPSASSHVNHEEFLPCLLHHSPDSKSITKLVYTPINTS 60

Query: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120
           +S +LNF  RN RFSTPNTPKPLLIITPSNISH+QAAV+CSKSHG+QIRIRSGGHDFEGL
Sbjct: 61  YSPVLNFSIRNLRFSTPNTPKPLLIITPSNISHVQAAVICSKSHGIQIRIRSGGHDFEGL 120

Query: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYVAYH F+VVDLINL SVT++VEQSTAWV+SGATLGELYY+I +KSRTL FPAG CPTV
Sbjct: 121 SYVAYHPFVVVDLINLSSVTIEVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNCPTV 180

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGG+G+LLRKYG+AADNVIDAY+VDANGEFHDR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYIVDANGEFHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAIFWNGGKVSA 300
           IVVAWKVKLVPVPATVTIC+  R+LEE+AIKLIDQWQYVANKL+EDLFL I   GGK+ A
Sbjct: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLIDQWQYVANKLDEDLFLGINLLGGKIVA 300

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q G KINPIALFF+LFLG  +ELMAILNKTFP+LGLTKEECKE SWIES     N  QIE
Sbjct: 301 Q-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360

Query: 361 DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRM 420
           DQPLE LLNRTP+ +G+ K+KSDYVKEP++KA I+ +W+RL+SQDIEG  L  IPYGGRM
Sbjct: 361 DQPLEVLLNRTPLANGNIKMKSDYVKEPISKATIKEVWQRLKSQDIEGANLVFIPYGGRM 420

Query: 421 NQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY 480
           +QISES+IPF HRAG LY+IGY+ GW E G+ AEK HLNWIR+IYSYMTPFV+KSPRAAY
Sbjct: 421 SQISESEIPFSHRAGNLYKIGYLTGWFEPGLNAEKTHLNWIRDIYSYMTPFVTKSPRAAY 480

Query: 481 VNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSNVDPYNFFWHEQSIPL 539
           VNYRDLDIGSN+KYGK +YK+A V+GSKYFG+NF+RLV VK+ VDPYNFF HEQSIP+
Sbjct: 481 VNYRDLDIGSNSKYGKTSYKRAHVWGSKYFGSNFDRLVYVKNEVDPYNFFRHEQSIPV 537

BLAST of CSPI01G25170 vs. NCBI nr
Match: gi|778662003|ref|XP_011659204.1| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 859.0 bits (2218), Expect = 4.5e-246
Identity = 415/544 (76.29%), Postives = 472/544 (86.76%), Query Frame = 1

Query: 1   MKLSPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLFHHSTDTYFITKLVHTPINSS 60
           MK S L+PLSL F+ VCSSSS+W +ASS +NHEEF+QCL HHS  +  I KL +TPIN+S
Sbjct: 1   MKFSLLLPLSLAFIAVCSSSSVWPSASSHVNHEEFLQCLLHHSPHSKSIAKLAYTPINTS 60

Query: 61  FSSLLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGL 120
           +SS+LNF  RN RFS PNTPKPL IITP+NISH+QAAV+CSKSHGLQIRIRSGGHDFEGL
Sbjct: 61  YSSVLNFSIRNLRFSIPNTPKPLFIITPTNISHVQAAVICSKSHGLQIRIRSGGHDFEGL 120

Query: 121 SYVAYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTV 180
           SYVAYH FI+VDLI+L SVT++V+QSTAWV+SGATLGELYY+I +KSRTL FPAG  PTV
Sbjct: 121 SYVAYHPFIIVDLIDLSSVTIEVKQSTAWVQSGATLGELYYRIAEKSRTLAFPAGNSPTV 180

Query: 181 GVGGHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHFSGGG+G+LLRKYG+AADNVIDAYLVDA+G FHDR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGFGTLLRKYGLAADNVIDAYLVDAHGVFHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAIFWNGGKVSA 300
           IVVAWKVKLVPVPATVTIC+  R+LEE+AIKL+DQWQYVANKL+EDLFL I   GGK+SA
Sbjct: 241 IVVAWKVKLVPVPATVTICTISRTLEEEAIKLVDQWQYVANKLDEDLFLGINLLGGKISA 300

Query: 301 QEGGKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIE 360
           Q G KINPIALFF+LFLG  +ELMAILNKTFP+LGLTKEECKE SWIES     N  QIE
Sbjct: 301 Q-GDKINPIALFFSLFLGKADELMAILNKTFPQLGLTKEECKETSWIESVVYTGNGLQIE 360

Query: 361 DQPLEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRM 420
           DQPLE LLNRTP+  G+ K+KSDYVKEP+ KA I+ IW+RLESQDIEG  L  +PYGGRM
Sbjct: 361 DQPLEVLLNRTPLATGNIKMKSDYVKEPIPKATIEEIWQRLESQDIEGANLVFVPYGGRM 420

Query: 421 NQISESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAY 480
           +QIS+S+IPF HRAG LY+IGY+ GW E GV AEK HLNWIR+IY YMTPFVSKSPRAAY
Sbjct: 421 SQISDSEIPFSHRAGNLYKIGYLTGWFEPGVNAEKTHLNWIRDIYGYMTPFVSKSPRAAY 480

Query: 481 VNYRDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSNVDPYNFFWHEQSIPLSP 540
           VNYRDLDIGSN+KYGK +YK+A V+G KYFGNNFNR+V VK+ VDPYNFF HEQSIP+  
Sbjct: 481 VNYRDLDIGSNSKYGKTSYKRARVWGLKYFGNNFNRMVYVKNKVDPYNFFRHEQSIPVLL 540

Query: 541 NHMV 545
            H +
Sbjct: 541 KHKI 543

BLAST of CSPI01G25170 vs. NCBI nr
Match: gi|778661997|ref|XP_004151192.2| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 765.4 bits (1975), Expect = 6.8e-218
Identity = 372/534 (69.66%), Postives = 444/534 (83.15%), Query Frame = 1

Query: 4   SPLIPLSLVFVVVCSSSSLWIAASSSINHEEFIQCLFHHSTDTYFITKLVHTPINSSFSS 63
           SPLIP SL+ +VV SSSSLW++AS    H  F+QCL  HS+ +Y I+K+VHTPINSS+SS
Sbjct: 5   SPLIPFSLILIVV-SSSSLWVSASPH-KHHNFLQCLSEHSSKSYPISKVVHTPINSSYSS 64

Query: 64  LLNFPTRNSRFSTPNTPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEGLSYV 123
           +LNF  RN RFS P TPKPLLIITPS++SHI+AAV+CSKSHGLQIR RSGGHD+EGLSYV
Sbjct: 65  VLNFSIRNLRFSKPETPKPLLIITPSHVSHIRAAVICSKSHGLQIRTRSGGHDYEGLSYV 124

Query: 124 AYHQFIVVDLINLKSVTVDVEQSTAWVESGATLGELYYKIGKKSRTLGFPAGICPTVGVG 183
           A H FI++DLINL+S+ +DVE +TAWVESG TLGELYY+IG+KSRTL FPAGICPTVGVG
Sbjct: 125 ASHPFIILDLINLRSMKIDVESNTAWVESGTTLGELYYRIGEKSRTLAFPAGICPTVGVG 184

Query: 184 GHFSGGGYGSLLRKYGVAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSFGIVV 243
           GHFSGGGYG +LRK+G+AADNVIDAYLVDANG+ HDRESMGEDLFWAIRGGGGGSFGIVV
Sbjct: 185 GHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVV 244

Query: 244 AWKVKLVPVPATVTICSAKRSLEEDAIKLIDQWQYVANKLEEDLFLAIFWNGGKVSAQEG 303
           AWK+KLV VPATVT+C+  R+L+ D IKL+ QWQYVANKL+E+LFL I   GG ++  + 
Sbjct: 245 AWKIKLVSVPATVTMCNTYRNLKGDGIKLVHQWQYVANKLDENLFLGIILTGGNITTTQE 304

Query: 304 GKINPIALFFTLFLGNVNELMAILNKTFPELGLTKEECKEKSWIESAASAANRFQIEDQP 363
           G  NPIA FF+LFLG VNEL+A L+ TFPELGL K++C E SWIES   A+   Q  +  
Sbjct: 305 GITNPIATFFSLFLGKVNELVATLSTTFPELGLIKQDCIETSWIESTLIASTGVQTVES- 364

Query: 364 LEALLNRTPITHGSFKLKSDYVKEPMTKAAIQGIWKRLESQDIEGVTLAVIPYGGRMNQI 423
           LE LLNRTP    + K+KSDY+KEP++ A I+GIW+RL++QDIE   L  IPYGGRM+QI
Sbjct: 365 LEPLLNRTPSNLENEKIKSDYIKEPISIATIEGIWQRLKAQDIETSQLIFIPYGGRMSQI 424

Query: 424 SESKIPFPHRAGILYQIGYILGWEEKGVEAEKRHLNWIREIYSYMTPFVSKSPRAAYVNY 483
           SES+ PF HR G LY+IGYILGW+E+ ++A+K+H++WIREIY YMTPFVSKSPR+AY NY
Sbjct: 425 SESETPFSHRVGNLYKIGYILGWKEQSLKAKKKHISWIREIYEYMTPFVSKSPRSAYANY 484

Query: 484 RDLDIGSNNKYGKINYKQACVFGSKYFGNNFNRLVKVKSNVDPYNFFWHEQSIP 538
           RDLDIG N KYGK + KQA ++G KYFGNNF RLV VK+ VDPY+FF HEQSIP
Sbjct: 485 RDLDIGVNKKYGKTSVKQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIP 535