CSPI01G25030 (gene) Wild cucumber (PI 183967)

NameCSPI01G25030
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionCleavage and polyadenylation specificity factor CPSF30
LocationChr1 : 20470320 .. 20470733 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGCCCCCACATATGCCAATGGGACGTGGTGCCAGACCCTTCCATGGAATGCAAAGTTTACCACCTGGGATGATGGGTCCGGATGGTTTGTCTTATGGGCCTGTTACGGCCGATGGATTTCCCATGCCTGACATTTTTGGTATGGCTCCTTGTGGTTTTGGTCCATATGGTCCTAGGTTTTCCGGTGATTTCATGGGTCCCTCATCTGCTATGACGTTTTGCGGACGACCTTCTCAACCTGGGGCCATGTTCACCCCTGGTGGGTTTGGGATGATGATGGGACAAGGACGTTGTCCCTTTATGGGTGGGATGGGAGTTACTGGTACTAGCCCAGCTCGACCTGGTCGTCCTGTGGGTGTTTCTCCATTGTATCCACCTCCAACAGGACCCTCAGCTCAGAACATCAATTGA

mRNA sequence

ATGTGGCCCCCACATATGCCAATGGGACGTGGTGCCAGACCCTTCCATGGAATGCAAAGTTTACCACCTGGGATGATGGGTCCGGATGGTTTGTCTTATGGGCCTGTTACGGCCGATGGATTTCCCATGCCTGACATTTTTGGTATGGCTCCTTGTGGTTTTGGTCCATATGGTCCTAGGTTTTCCGGTGATTTCATGGGTCCCTCATCTGCTATGACGTTTTGCGGACGACCTTCTCAACCTGGGGCCATGTTCACCCCTGGTGGGTTTGGGATGATGATGGGACAAGGACGTTGTCCCTTTATGGGTGGGATGGGAGTTACTGGTACTAGCCCAGCTCGACCTGGTCGTCCTGTGGGTGTTTCTCCATTGTATCCACCTCCAACAGGACCCTCAGCTCAGAACATCAATTGA

Coding sequence (CDS)

ATGTGGCCCCCACATATGCCAATGGGACGTGGTGCCAGACCCTTCCATGGAATGCAAAGTTTACCACCTGGGATGATGGGTCCGGATGGTTTGTCTTATGGGCCTGTTACGGCCGATGGATTTCCCATGCCTGACATTTTTGGTATGGCTCCTTGTGGTTTTGGTCCATATGGTCCTAGGTTTTCCGGTGATTTCATGGGTCCCTCATCTGCTATGACGTTTTGCGGACGACCTTCTCAACCTGGGGCCATGTTCACCCCTGGTGGGTTTGGGATGATGATGGGACAAGGACGTTGTCCCTTTATGGGTGGGATGGGAGTTACTGGTACTAGCCCAGCTCGACCTGGTCGTCCTGTGGGTGTTTCTCCATTGTATCCACCTCCAACAGGACCCTCAGCTCAGAACATCAATTGA
BLAST of CSPI01G25030 vs. Swiss-Prot
Match: CPSF_ARATH (30-kDa cleavage and polyadenylation specificity factor 30 OS=Arabidopsis thaliana GN=CPSF30 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.0e-25
Identity = 72/135 (53.33%), Postives = 78/135 (57.78%), Query Frame = 1

Query: 1   MWPPHMPMGRGARPFHGMQSLPPGMMGP-DGLSYGPVTADGFPMPDIFGMAPCGFGPYGP 60
           MWPP MP+GRG RP  GM   P G+MGP D   YGP   +G  MPD FGM P  FGPYGP
Sbjct: 447 MWPPQMPLGRGIRPMPGMGGFPLGVMGPGDAFPYGPGGYNG--MPDPFGMGPRPFGPYGP 506

Query: 61  RFSGDFMGPSSAMTFCGRPSQPGAMFTPGGFGMMMGQGRCPFMGGMGVTGTSPARPGRPV 120
           RF GDF GP   M F GRP Q    F  GG+G MMG GR P MGGMG           P 
Sbjct: 507 RFGGDFRGPVPGMMFPGRPPQ---QFPHGGYG-MMGGGRGPHMGGMG---------NAPR 566

Query: 121 GVSPLYPPPTGPSAQ 135
           G  P+Y PP   SA+
Sbjct: 567 GGRPMYYPPATSSAR 566

BLAST of CSPI01G25030 vs. TrEMBL
Match: A0A0A0LY24_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G538270 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 8.4e-71
Identity = 130/137 (94.89%), Postives = 131/137 (95.62%), Query Frame = 1

Query: 1   MWPPHMPMGRGARPFHGMQSLPPGMMGPDGLSYGPVTADGFPMPDIFGMAPCGFGPYGPR 60
           MW PHMPMGRGARPFHGMQS PPGMMGPDGLSYGPVTADGFP+PDIFGMAPCGFGPYGPR
Sbjct: 424 MWTPHMPMGRGARPFHGMQSFPPGMMGPDGLSYGPVTADGFPVPDIFGMAPCGFGPYGPR 483

Query: 61  FSGDFMGPSSAMTFCGRPSQPGAMFTPGGFGMMMGQGRCPFMGGMGVTGTSPARPGRPVG 120
           FSGDFMGPSSAM F GRPSQPGAMFTPG FGMMMGQGR PFMGGMGVTGTSPARPGRPVG
Sbjct: 484 FSGDFMGPSSAMMFRGRPSQPGAMFTPGEFGMMMGQGRGPFMGGMGVTGTSPARPGRPVG 543

Query: 121 VSPLYPPPTGPSAQNIN 138
           VSPLYPPPTGPSAQNIN
Sbjct: 544 VSPLYPPPTGPSAQNIN 560

BLAST of CSPI01G25030 vs. TrEMBL
Match: A0A0A0KSR6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G647360 PE=4 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 7.1e-62
Identity = 117/139 (84.17%), Postives = 121/139 (87.05%), Query Frame = 1

Query: 1   MWPPHMPMGRGARPFHGMQSLPPGMMGPDGLSYGPVTADGFPMPDIFGMAPCGFGPYGP- 60
           MWPPHMPMGRGARPFHGMQ  PPGMMGPDGLSYGPVT DGFPMPDIFGM P GFGPYGP 
Sbjct: 476 MWPPHMPMGRGARPFHGMQGFPPGMMGPDGLSYGPVTPDGFPMPDIFGMTPRGFGPYGPT 535

Query: 61  -RFSGDFMGPSSAMTFCGRPSQPGAMFTPGGFGMMMGQGRCPFMGGMGVTGTSPARPGRP 120
            RFSGDFMGP +AM F GRPSQP AMF P GFGMMMGQGR PFMGGMGV G +PARPGRP
Sbjct: 536 PRFSGDFMGPPTAMMFRGRPSQPAAMFPPSGFGMMMGQGRGPFMGGMGVAGANPARPGRP 595

Query: 121 VGVSPLYPPPTGPSAQNIN 138
           VGVSPLYPPP  PS+QN+N
Sbjct: 596 VGVSPLYPPPAVPSSQNMN 614

BLAST of CSPI01G25030 vs. TrEMBL
Match: A0A061DY09_THECC (Cleavage and polyadenylation specificity factor 30 OS=Theobroma cacao GN=TCM_006095 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 5.5e-46
Identity = 93/135 (68.89%), Postives = 103/135 (76.30%), Query Frame = 1

Query: 1   MWPPHMPMGRGARPFHGMQSLPPGMMGPDGLSYGPVTADGFPMPDIFGMAPCGFGPYGPR 60
           MWPPHMP+ RGARP  GM+  PP MMG DG SYGPVT DGF +PD+FG AP  F PYGPR
Sbjct: 467 MWPPHMPLARGARPMPGMRGFPPMMMGGDGFSYGPVTPDGFGVPDLFG-APRPFPPYGPR 526

Query: 61  FSGDFMGPSSAMTFCGRPSQPGAMFTPGGFGMMMGQGRCPFMGGMGVTGTSPARPGRPVG 120
           FSGDF GP+S M F GRP QPGAMF  GG GMMMG GR PFMGGMG TG +P R GRPV 
Sbjct: 527 FSGDFTGPASGMMFPGRPPQPGAMFPAGGLGMMMGPGRAPFMGGMGPTGANPVRGGRPVS 586

Query: 121 VSPLYPPPTGPSAQN 136
           + P++PPP  PS+QN
Sbjct: 587 MPPMFPPPPAPSSQN 600

BLAST of CSPI01G25030 vs. TrEMBL
Match: A0A0D2R9E9_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_008G046800 PE=4 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 7.2e-46
Identity = 93/135 (68.89%), Postives = 103/135 (76.30%), Query Frame = 1

Query: 1   MWPPHMPMGRGARPFHGMQSLPPGMMGPDGLSYGPVTADGFPMPDIFGMAPCGFGPYGPR 60
           MWPPHMP+ RGARP  GM+  PP MMG DG SYGPVT DGF MPD+FG AP  F PYGPR
Sbjct: 470 MWPPHMPLARGARPMPGMRGFPPMMMGGDGFSYGPVTPDGFGMPDLFG-APRPFAPYGPR 529

Query: 61  FSGDFMGPSSAMTFCGRPSQPGAMFTPGGFGMMMGQGRCPFMGGMGVTGTSPARPGRPVG 120
           FSGDF GP+S M F GRP QPG MF  GG GMMMG GR PFMGGMG TG +PAR GRPVG
Sbjct: 530 FSGDFTGPASGMMFPGRPPQPGGMFPSGGIGMMMGPGRAPFMGGMGPTGANPARGGRPVG 589

Query: 121 VSPLYPPPTGPSAQN 136
           + P++P P  P++QN
Sbjct: 590 MPPMFPLPPAPASQN 603

BLAST of CSPI01G25030 vs. TrEMBL
Match: A0A0D2PNT4_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_008G046800 PE=4 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 7.2e-46
Identity = 93/135 (68.89%), Postives = 103/135 (76.30%), Query Frame = 1

Query: 1   MWPPHMPMGRGARPFHGMQSLPPGMMGPDGLSYGPVTADGFPMPDIFGMAPCGFGPYGPR 60
           MWPPHMP+ RGARP  GM+  PP MMG DG SYGPVT DGF MPD+FG AP  F PYGPR
Sbjct: 469 MWPPHMPLARGARPMPGMRGFPPMMMGGDGFSYGPVTPDGFGMPDLFG-APRPFAPYGPR 528

Query: 61  FSGDFMGPSSAMTFCGRPSQPGAMFTPGGFGMMMGQGRCPFMGGMGVTGTSPARPGRPVG 120
           FSGDF GP+S M F GRP QPG MF  GG GMMMG GR PFMGGMG TG +PAR GRPVG
Sbjct: 529 FSGDFTGPASGMMFPGRPPQPGGMFPSGGIGMMMGPGRAPFMGGMGPTGANPARGGRPVG 588

Query: 121 VSPLYPPPTGPSAQN 136
           + P++P P  P++QN
Sbjct: 589 MPPMFPLPPAPASQN 602

BLAST of CSPI01G25030 vs. TAIR10
Match: AT1G30460.1 (AT1G30460.1 cleavage and polyadenylation specificity factor 30)

HSP 1 Score: 116.7 bits (291), Expect = 1.1e-26
Identity = 72/135 (53.33%), Postives = 78/135 (57.78%), Query Frame = 1

Query: 1   MWPPHMPMGRGARPFHGMQSLPPGMMGP-DGLSYGPVTADGFPMPDIFGMAPCGFGPYGP 60
           MWPP MP+GRG RP  GM   P G+MGP D   YGP   +G  MPD FGM P  FGPYGP
Sbjct: 447 MWPPQMPLGRGIRPMPGMGGFPLGVMGPGDAFPYGPGGYNG--MPDPFGMGPRPFGPYGP 506

Query: 61  RFSGDFMGPSSAMTFCGRPSQPGAMFTPGGFGMMMGQGRCPFMGGMGVTGTSPARPGRPV 120
           RF GDF GP   M F GRP Q    F  GG+G MMG GR P MGGMG           P 
Sbjct: 507 RFGGDFRGPVPGMMFPGRPPQ---QFPHGGYG-MMGGGRGPHMGGMG---------NAPR 566

Query: 121 GVSPLYPPPTGPSAQ 135
           G  P+Y PP   SA+
Sbjct: 567 GGRPMYYPPATSSAR 566

BLAST of CSPI01G25030 vs. NCBI nr
Match: gi|778665246|ref|XP_011648522.1| (PREDICTED: LOW QUALITY PROTEIN: 30-kDa cleavage and polyadenylation specificity factor 30, partial [Cucumis sativus])

HSP 1 Score: 274.2 bits (700), Expect = 1.2e-70
Identity = 130/137 (94.89%), Postives = 131/137 (95.62%), Query Frame = 1

Query: 1   MWPPHMPMGRGARPFHGMQSLPPGMMGPDGLSYGPVTADGFPMPDIFGMAPCGFGPYGPR 60
           MW PHMPMGRGARPFHGMQS PPGMMGPDGLSYGPVTADGFP+PDIFGMAPCGFGPYGPR
Sbjct: 481 MWTPHMPMGRGARPFHGMQSFPPGMMGPDGLSYGPVTADGFPVPDIFGMAPCGFGPYGPR 540

Query: 61  FSGDFMGPSSAMTFCGRPSQPGAMFTPGGFGMMMGQGRCPFMGGMGVTGTSPARPGRPVG 120
           FSGDFMGPSSAM F GRPSQPGAMFTPG FGMMMGQGR PFMGGMGVTGTSPARPGRPVG
Sbjct: 541 FSGDFMGPSSAMMFRGRPSQPGAMFTPGEFGMMMGQGRGPFMGGMGVTGTSPARPGRPVG 600

Query: 121 VSPLYPPPTGPSAQNIN 138
           VSPLYPPPTGPSAQNIN
Sbjct: 601 VSPLYPPPTGPSAQNIN 617

BLAST of CSPI01G25030 vs. NCBI nr
Match: gi|700210833|gb|KGN65929.1| (hypothetical protein Csa_1G538270 [Cucumis sativus])

HSP 1 Score: 274.2 bits (700), Expect = 1.2e-70
Identity = 130/137 (94.89%), Postives = 131/137 (95.62%), Query Frame = 1

Query: 1   MWPPHMPMGRGARPFHGMQSLPPGMMGPDGLSYGPVTADGFPMPDIFGMAPCGFGPYGPR 60
           MW PHMPMGRGARPFHGMQS PPGMMGPDGLSYGPVTADGFP+PDIFGMAPCGFGPYGPR
Sbjct: 424 MWTPHMPMGRGARPFHGMQSFPPGMMGPDGLSYGPVTADGFPVPDIFGMAPCGFGPYGPR 483

Query: 61  FSGDFMGPSSAMTFCGRPSQPGAMFTPGGFGMMMGQGRCPFMGGMGVTGTSPARPGRPVG 120
           FSGDFMGPSSAM F GRPSQPGAMFTPG FGMMMGQGR PFMGGMGVTGTSPARPGRPVG
Sbjct: 484 FSGDFMGPSSAMMFRGRPSQPGAMFTPGEFGMMMGQGRGPFMGGMGVTGTSPARPGRPVG 543

Query: 121 VSPLYPPPTGPSAQNIN 138
           VSPLYPPPTGPSAQNIN
Sbjct: 544 VSPLYPPPTGPSAQNIN 560

BLAST of CSPI01G25030 vs. NCBI nr
Match: gi|659068585|ref|XP_008445183.1| (PREDICTED: cleavage and polyadenylation specificity factor CPSF30 [Cucumis melo])

HSP 1 Score: 268.1 bits (684), Expect = 8.6e-69
Identity = 128/137 (93.43%), Postives = 128/137 (93.43%), Query Frame = 1

Query: 1   MWPPHMPMGRGARPFHGMQSLPPGMMGPDGLSYGPVTADGFPMPDIFGMAPCGFGPYGPR 60
           MWPPHMPMGRGARPFHGMQS PPGMMGPDGLSYGPVT DGFPMPDIFGMAP GFGPYGPR
Sbjct: 478 MWPPHMPMGRGARPFHGMQSFPPGMMGPDGLSYGPVTPDGFPMPDIFGMAPRGFGPYGPR 537

Query: 61  FSGDFMGPSSAMTFCGRPSQPGAMFTPGGFGMMMGQGRCPFMGGMGVTGTSPARPGRPVG 120
           FSGDFMGP SAM F GRPSQPGAMFTPGGFGMMMGQGR PFMGGMGVTGTSPARPGRPVG
Sbjct: 538 FSGDFMGPPSAMMFRGRPSQPGAMFTPGGFGMMMGQGRGPFMGGMGVTGTSPARPGRPVG 597

Query: 121 VSPLYPPPTGPSAQNIN 138
           VSPLYPPP  PSAQNIN
Sbjct: 598 VSPLYPPPAVPSAQNIN 614

BLAST of CSPI01G25030 vs. NCBI nr
Match: gi|449447537|ref|XP_004141524.1| (PREDICTED: 30-kDa cleavage and polyadenylation specificity factor 30-like [Cucumis sativus])

HSP 1 Score: 244.6 bits (623), Expect = 1.0e-61
Identity = 117/139 (84.17%), Postives = 121/139 (87.05%), Query Frame = 1

Query: 1   MWPPHMPMGRGARPFHGMQSLPPGMMGPDGLSYGPVTADGFPMPDIFGMAPCGFGPYGP- 60
           MWPPHMPMGRGARPFHGMQ  PPGMMGPDGLSYGPVT DGFPMPDIFGM P GFGPYGP 
Sbjct: 476 MWPPHMPMGRGARPFHGMQGFPPGMMGPDGLSYGPVTPDGFPMPDIFGMTPRGFGPYGPT 535

Query: 61  -RFSGDFMGPSSAMTFCGRPSQPGAMFTPGGFGMMMGQGRCPFMGGMGVTGTSPARPGRP 120
            RFSGDFMGP +AM F GRPSQP AMF P GFGMMMGQGR PFMGGMGV G +PARPGRP
Sbjct: 536 PRFSGDFMGPPTAMMFRGRPSQPAAMFPPSGFGMMMGQGRGPFMGGMGVAGANPARPGRP 595

Query: 121 VGVSPLYPPPTGPSAQNIN 138
           VGVSPLYPPP  PS+QN+N
Sbjct: 596 VGVSPLYPPPAVPSSQNMN 614

BLAST of CSPI01G25030 vs. NCBI nr
Match: gi|659119181|ref|XP_008459517.1| (PREDICTED: cleavage and polyadenylation specificity factor CPSF30-like [Cucumis melo])

HSP 1 Score: 241.1 bits (614), Expect = 1.1e-60
Identity = 118/140 (84.29%), Postives = 123/140 (87.86%), Query Frame = 1

Query: 1   MWPPHMPMGRGARPFHGMQSLPPGMMGPDGLSYGPVTADGFPMPDIFGMAPCGFGPYG-- 60
           MWPP MP+GRGARPFHGMQ  PPGMMGPDGLSYGPVT DGFPMPDIFGMAP GFGPYG  
Sbjct: 476 MWPPQMPIGRGARPFHGMQGFPPGMMGPDGLSYGPVTPDGFPMPDIFGMAPRGFGPYGPT 535

Query: 61  PRFSGDFMGPSSAMTFCGRPSQPGAMFTPGGFGMMMGQGR-CPFMGGMGVTGTSPARPGR 120
           PRFS DFMGP +AM F GRPSQPGAMF PGGFGMMMGQGR  PFMGGMGVTG +PARPGR
Sbjct: 536 PRFSSDFMGPPTAMMFRGRPSQPGAMFPPGGFGMMMGQGRGGPFMGGMGVTGANPARPGR 595

Query: 121 PVGVSPLYPPPTGPSAQNIN 138
           PVGVSPLYPPP  PS+QN+N
Sbjct: 596 PVGVSPLYPPPAVPSSQNMN 615

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CPSF_ARATH2.0e-2553.3330-kDa cleavage and polyadenylation specificity factor 30 OS=Arabidopsis thalian... [more]
Match NameE-valueIdentityDescription
A0A0A0LY24_CUCSA8.4e-7194.89Uncharacterized protein OS=Cucumis sativus GN=Csa_1G538270 PE=4 SV=1[more]
A0A0A0KSR6_CUCSA7.1e-6284.17Uncharacterized protein OS=Cucumis sativus GN=Csa_5G647360 PE=4 SV=1[more]
A0A061DY09_THECC5.5e-4668.89Cleavage and polyadenylation specificity factor 30 OS=Theobroma cacao GN=TCM_006... [more]
A0A0D2R9E9_GOSRA7.2e-4668.89Uncharacterized protein OS=Gossypium raimondii GN=B456_008G046800 PE=4 SV=1[more]
A0A0D2PNT4_GOSRA7.2e-4668.89Uncharacterized protein OS=Gossypium raimondii GN=B456_008G046800 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G30460.11.1e-2653.33 cleavage and polyadenylation specificity factor 30[more]
Match NameE-valueIdentityDescription
gi|778665246|ref|XP_011648522.1|1.2e-7094.89PREDICTED: LOW QUALITY PROTEIN: 30-kDa cleavage and polyadenylation specificity ... [more]
gi|700210833|gb|KGN65929.1|1.2e-7094.89hypothetical protein Csa_1G538270 [Cucumis sativus][more]
gi|659068585|ref|XP_008445183.1|8.6e-6993.43PREDICTED: cleavage and polyadenylation specificity factor CPSF30 [Cucumis melo][more]
gi|449447537|ref|XP_004141524.1|1.0e-6184.17PREDICTED: 30-kDa cleavage and polyadenylation specificity factor 30-like [Cucum... [more]
gi|659119181|ref|XP_008459517.1|1.1e-6084.29PREDICTED: cleavage and polyadenylation specificity factor CPSF30-like [Cucumis ... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051716 cellular response to stimulus
biological_process GO:0006378 mRNA polyadenylation
biological_process GO:0044763 single-organism cellular process
biological_process GO:0050896 response to stimulus
biological_process GO:0050794 regulation of cellular process
biological_process GO:0031124 mRNA 3'-end processing
biological_process GO:0008150 biological_process
biological_process GO:0006950 response to stress
biological_process GO:0048583 regulation of response to stimulus
biological_process GO:0043068 positive regulation of programmed cell death
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
cellular_component GO:0005634 nucleus
cellular_component GO:0044424 intracellular part
cellular_component GO:0005575 cellular_component
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0003723 RNA binding
molecular_function GO:0046872 metal ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G25030.1CSPI01G25030.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR12357YTH YT521-B HOMOLOGY DOMAIN-CONTAININGcoord: 1..78
score: 5.0
NoneNo IPR availablePANTHERPTHR12357:SF3FI21232P1coord: 1..78
score: 5.0

The following gene(s) are paralogous to this gene:

None