CSPI01G17060 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI01G17060
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Chr1 : 12856286 .. 12856924 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATAATTGAGACGGTGCTGATAGCCATGGCAAGATCCCTAGACGTGGAGCCCAATAGCTTTACCGATCAGGTAGGGAAGCGACCAACTTTACTCACAAGGTTCAATTTCTATCCCCCGTGTTCGACGCCACATCTTGTTCTTGGACTCAAAGAACACTCCGATGGTTCAGCTATCACCATTCTTCTACTCGACAAACAAGTTGAAGGCCTCCAATTGCGAAAGGATGATCAGTGGTATAGAGTACCTGTTCCTGCCATTGCTGATTCTCTCCTCATCATCATTGGGGAACAAGCCGAAGTAACAATATTAAATACTTATTTTTATTATTATTATTATCTTTATGTTACATACTGGGTTTTTAAAGGATTATTAATGTAATGTGTTATGTGCATGGAGGTTATGAGCAATGGAATCTTCAAGAGCTCTATTCATCGGGCGGTGACGAATTCAGAGAGGCAGAGGATTTCATTGGTGTGTTTCTGCTGCCCAGAAAAAGATATTGAGATCAAGCCAATCGAGGGGCTGATCGACGAGAAGAGACCGAGATTGTTCAAAAGTGTGAAGAACTATTTGGAAACTTATTTTCAGAACTACCAAAAGGGACAGAGACCAGTTGATGGATTGAGGATTTAG

mRNA sequence

ATGGAAATAATTGAGACGGTGCTGATAGCCATGGCAAGATCCCTAGACGTGGAGCCCAATAGCTTTACCGATCAGGTAGGGAAGCGACCAACTTTACTCACAAGGTTCAATTTCTATCCCCCGTGTTCGACGCCACATCTTGTTCTTGGACTCAAAGAACACTCCGATGGTTCAGCTATCACCATTCTTCTACTCGACAAACAAGTTGAAGGCCTCCAATTGCGAAAGGATGATCAGTGGTATAGAGTACCTGTTCCTGCCATTGCTGATTCTCTCCTCATCATCATTGGGGAACAAGCCGAAGTTATGAGCAATGGAATCTTCAAGAGCTCTATTCATCGGGCGGTGACGAATTCAGAGAGGCAGAGGATTTCATTGGTGTGTTTCTGCTGCCCAGAAAAAGATATTGAGATCAAGCCAATCGAGGGGCTGATCGACGAGAAGAGACCGAGATTGTTCAAAAGTGTGAAGAACTATTTGGAAACTTATTTTCAGAACTACCAAAAGGGACAGAGACCAGTTGATGGATTGAGGATTTAG

Coding sequence (CDS)

ATGGAAATAATTGAGACGGTGCTGATAGCCATGGCAAGATCCCTAGACGTGGAGCCCAATAGCTTTACCGATCAGGTAGGGAAGCGACCAACTTTACTCACAAGGTTCAATTTCTATCCCCCGTGTTCGACGCCACATCTTGTTCTTGGACTCAAAGAACACTCCGATGGTTCAGCTATCACCATTCTTCTACTCGACAAACAAGTTGAAGGCCTCCAATTGCGAAAGGATGATCAGTGGTATAGAGTACCTGTTCCTGCCATTGCTGATTCTCTCCTCATCATCATTGGGGAACAAGCCGAAGTTATGAGCAATGGAATCTTCAAGAGCTCTATTCATCGGGCGGTGACGAATTCAGAGAGGCAGAGGATTTCATTGGTGTGTTTCTGCTGCCCAGAAAAAGATATTGAGATCAAGCCAATCGAGGGGCTGATCGACGAGAAGAGACCGAGATTGTTCAAAAGTGTGAAGAACTATTTGGAAACTTATTTTCAGAACTACCAAAAGGGACAGAGACCAGTTGATGGATTGAGGATTTAG

Homology

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BLAST of CSPI01G17060 vs. Swiss-Prot
Match: DIOX2_PAPSO (Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum GN=DIOX2 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 5.3e-26
Identity = 61/174 (35.06%), Postives = 100/174 (57.47%), Query Frame = 1


Query: 11  MARSLDVEPNSFTD--QVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAITILLLDKQ 70
           M ++L V+     +  +V K  T + R N+YPPC  P L +GL  HSD   +TILL   +
Sbjct: 193 MEKALQVQAVEIKEISEVFKDMTQVMRMNYYPPCPQPELAIGLTPHSDFGGLTILLQLNE 252

Query: 71  VEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSERQRISLVC 130
           VEGLQ++ + +W  + V  + ++ ++ +G+  E+M+NG+++S  HRAV NS ++R+S+  
Sbjct: 253 VEGLQIKNEGRW--ISVKPLPNAFVVNVGDVLEIMTNGMYRSVDHRAVVNSTKERLSIAT 312

Query: 131 FCCPEKDIEIKPIEGLIDEKRPRLFKSVKNY---LETYFQNYQKGQRPVDGLRI 180
           F  P  + EI PI  LI    P LF+S   Y   +E +      G+  +D +R+
Sbjct: 313 FHDPNLESEIGPISSLITPNTPALFRSGSTYGELVEEFHSRKLDGKSFLDSMRM 364

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BLAST of CSPI01G17060 vs. Swiss-Prot
Match: FLS_EUSER (Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellianum GN=FLS PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 5.3e-26
Identity = 64/156 (41.03%), Postives = 94/156 (60.26%), Query Frame = 1


Query: 3   IIETVLIAMARSLDVEPNSFTDQVGKRPTL-LTRFNFYPPCSTPHLVLGLKEHSDGSAIT 62
           +++ +   ++  LD+EPNSF D  G    + L + N+YPPC  P L LG+  H+D SAIT
Sbjct: 169 VVDNLFKYLSLGLDLEPNSFKDGAGGDDLVYLMKINYYPPCPRPDLALGVA-HTDMSAIT 228

Query: 63  ILLLDKQVEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSER 122
           +L+   +V GLQ+ KD  WY      I ++L++ IG+Q E+MSNG +KS  HR   N E+
Sbjct: 229 VLV-PNEVPGLQVYKDGHWYDCKY--IPNALIVHIGDQVEIMSNGKYKSVYHRTTVNKEK 288

Query: 123 QRISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVK 158
            R+S   F  P  D E+ PI  L++E+ P  FK+ K
Sbjct: 289 TRMSWPVFLEPPPDHEVGPIPKLVNEENPAKFKTKK 320

[Close]

BLAST of CSPI01G17060 vs. Swiss-Prot
Match: SRG1_ARATH (Protein SRG1 OS=Arabidopsis thaliana GN=SRG1 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 9.0e-26
Identity = 64/185 (34.59%), Postives = 108/185 (58.38%), Query Frame = 1


Query: 1   MEIIETVLIA-MARSLDVEPNSFT---DQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSD 60
           ++ +  +LIA MAR+L+++P       D V    ++  R N+YPPC  P  V+GL  HSD
Sbjct: 178 VQSVAKILIAKMARALEIKPEELEKLFDDVDSVQSM--RMNYYPPCPQPDQVIGLTPHSD 237

Query: 61  GSAITILLLDKQVEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAV 120
              +T+L+    VEGLQ++KD +W  VPV  + ++ ++ IG+  E+++NG ++S  HR V
Sbjct: 238 SVGLTVLMQVNDVEGLQIKKDGKW--VPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGV 297

Query: 121 TNSERQRISLVCFCCPEKDIEIKPIEGLIDEKRPRLFK--SVKNYLETYFQNYQKGQRPV 180
            NSE++R+S+  F       E+ P + L++ ++   FK  ++K Y +  F     G+  +
Sbjct: 298 VNSEKERLSIATFHNVGMYKEVGPAKSLVERQKVARFKRLTMKEYNDGLFSRTLDGKAYL 357

[Close]

BLAST of CSPI01G17060 vs. Swiss-Prot
Match: DIOX3_PAPSO (Codeine O-demethylase OS=Papaver somniferum GN=CODM PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.5e-25
Identity = 54/120 (45.00%), Postives = 81/120 (67.50%), Query Frame = 1


Query: 35  RFNFYPPCSTPHLVLGLKEHSDGSAITILLLDKQVEGLQLRKDDQWYRVPVPAIADSLLI 94
           R N+YPPC  P LVLGL  HSD S +TILL   +VEGLQ+RK+++W  + +  + D+ ++
Sbjct: 216 RMNYYPPCPRPELVLGLTSHSDFSGLTILLQLNEVEGLQIRKEERW--ISIKPLPDAFIV 275

Query: 95  IIGEQAEVMSNGIFKSSIHRAVTNSERQRISLVCFCCPEKDIEIKPIEGLIDEKRPRLFK 154
            +G+  E+M+NGI++S  HRAV NS ++R+S+  F   + + EI PI  L+  + P LFK
Sbjct: 276 NVGDILEIMTNGIYRSVEHRAVVNSTKERLSIATFHDSKLESEIGPISSLVTPETPALFK 333

[Close]

BLAST of CSPI01G17060 vs. Swiss-Prot
Match: DLO1_ARATH (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana GN=DLO1 PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.1e-23
Identity = 59/146 (40.41%), Postives = 91/146 (62.33%), Query Frame = 1


Query: 3   IIETVLIAMARSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAITI 62
           ++  +L A++ SL +E +  ++ +GK    +  FN+YPPC  P L  GL  H D + IT+
Sbjct: 171 LVLRLLEAISESLGLESDHISNILGKHAQHMA-FNYYPPCPEPELTYGLPGHKDPTVITV 230

Query: 63  LLLDKQVEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSERQ 122
           LL D QV GLQ+ KDD+W  V V  I ++ ++ IG+Q +V+SN  +KS +HRAV N+E +
Sbjct: 231 LLQD-QVSGLQVFKDDKW--VAVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENE 290

Query: 123 RISLVCFCCPEKDIEIKPIEGLIDEK 149
           R+S+  F  P  D  I P   L++E+
Sbjct: 291 RLSIPTFYFPSTDAVIGPAHELVNEQ 312

[Close]

BLAST of CSPI01G17060 vs. TrEMBL
Match: A0A0A0LW33_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G263970 PE=4 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 1.2e-96
Identity = 175/179 (97.77%), Postives = 178/179 (99.44%), Query Frame = 1


Query: 1   MEIIETVLIAMARSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAI 60
           MEIIETVLIAMA SLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAI
Sbjct: 20  MEIIETVLIAMASSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAI 79

Query: 61  TILLLDKQVEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSE 120
           TILLLDKQVEGLQLRKDDQWYRVPVPAIADSLL++IGEQAEVMSNGIFKSSIHRAVTNSE
Sbjct: 80  TILLLDKQVEGLQLRKDDQWYRVPVPAIADSLLLVIGEQAEVMSNGIFKSSIHRAVTNSE 139

Query: 121 RQRISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKGQRPVDGLRI 180
           RQRISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKG+RPVDGLRI
Sbjct: 140 RQRISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKGRRPVDGLRI 198

[Close]

BLAST of CSPI01G17060 vs. TrEMBL
Match: A0A0A0LW96_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G212830 PE=3 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 5.4e-94
Identity = 168/179 (93.85%), Postives = 177/179 (98.88%), Query Frame = 1


Query: 1   MEIIETVLIAMARSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAI 60
           MEIIETVLIAMARSL+VEPNSFTDQVG+RPTL TRFNFYPPCSTPHLVLGLKEHSDGSAI
Sbjct: 399 MEIIETVLIAMARSLNVEPNSFTDQVGERPTLFTRFNFYPPCSTPHLVLGLKEHSDGSAI 458

Query: 61  TILLLDKQVEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSE 120
           TILLLDKQVEGLQLRKDDQWYRVPVPAIADSLL++IGEQAEVMSNGIFKSS+HRAVTNSE
Sbjct: 459 TILLLDKQVEGLQLRKDDQWYRVPVPAIADSLLLVIGEQAEVMSNGIFKSSVHRAVTNSE 518

Query: 121 RQRISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKGQRPVDGLRI 180
           RQRIS+VCFCCPEKDIEIKP+EGLIDEKRPRLF+SVKNYLETYFQNYQ+GQR VDGLRI
Sbjct: 519 RQRISVVCFCCPEKDIEIKPVEGLIDEKRPRLFRSVKNYLETYFQNYQEGQRTVDGLRI 577

[Close]

BLAST of CSPI01G17060 vs. TrEMBL
Match: U7E2D8_POPTR (Oxidoreductase family protein OS=Populus trichocarpa GN=POPTR_0025s00440g PE=3 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.1e-54
Identity = 100/177 (56.50%), Postives = 136/177 (76.84%), Query Frame = 1


Query: 3   IIETVLIAMARSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAITI 62
           I+E VL AMARSL++E N F D+ G+R  +  RFNF+PPC  P   LGLK H+DGSAITI
Sbjct: 178 IVEVVLKAMARSLNLEDNCFLDKYGERALMQARFNFFPPCPRPDRSLGLKPHADGSAITI 237

Query: 63  LLLDKQVEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSERQ 122
           +L DK+VEGLQ  KDDQW+RVP+  +  +LLI +G+Q+EVMSNG FKS +HR VTNSER+
Sbjct: 238 VLQDKEVEGLQFLKDDQWFRVPIQ-LPHALLINVGDQSEVMSNGFFKSPVHRVVTNSERE 297

Query: 123 RISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKGQRPVDGLRI 180
           R S+  FC P+ D +I+P++G + E RPRL+K V++Y+  YFQ YQ+G+RP++ ++I
Sbjct: 298 RTSVAVFCSPDPDNDIEPVDGAVSETRPRLYKKVQDYVSKYFQYYQEGKRPIEAVKI 353

[Close]

BLAST of CSPI01G17060 vs. TrEMBL
Match: B9N703_POPTR (Oxidoreductase family protein OS=Populus trichocarpa GN=POPTR_0006s06130g PE=3 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 1.4e-54
Identity = 102/178 (57.30%), Postives = 135/178 (75.84%), Query Frame = 1


Query: 2   EIIETVLIAMARSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAIT 61
           EI E +L AMA SL++E +SF DQ G+RP +  RFNFYPPC  P  +LG+K H+D SAIT
Sbjct: 172 EINEILLRAMAMSLNLEESSFLDQYGERPLVAARFNFYPPCPRPDRILGVKPHADASAIT 231

Query: 62  ILLLDKQVEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSER 121
            LL DK+VEGLQ  KD+QW+RVP+  I  +LLI +G+Q E+MSNGIFKS +HR VTN+E+
Sbjct: 232 FLLQDKEVEGLQFLKDNQWFRVPI--IPHALLINVGDQVEIMSNGIFKSPVHRVVTNTEK 291

Query: 122 QRISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKGQRPVDGLRI 180
           +R +L  FC P+ D EIKP +GLI E RP L+K VK+Y+  YFQ YQ+G+RP++ ++I
Sbjct: 292 ERNTLAVFCIPDSDKEIKPADGLISETRPSLYKKVKDYVSIYFQYYQQGKRPIEAVKI 347

[Close]

BLAST of CSPI01G17060 vs. TrEMBL
Match: M5WFY4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007960mg PE=3 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 5.5e-54
Identity = 98/177 (55.37%), Postives = 136/177 (76.84%), Query Frame = 1


Query: 3   IIETVLIAMARSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAITI 62
           + +TVL AMARSL+++ N F D  G++  +  RFNFYPPCS P +VLG+K H+DG+ IT+
Sbjct: 176 VTKTVLEAMARSLNLDVNCFRDLCGEQGKMDVRFNFYPPCSRPDVVLGVKPHADGTIITL 235

Query: 63  LLLDKQVEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSERQ 122
           LL DKQVEGLQ  KDDQW+R P+  + ++LLI +G+QAE++SNGIFKS +HR VTN +++
Sbjct: 236 LLQDKQVEGLQFLKDDQWFRAPI--VPEALLINVGDQAEILSNGIFKSPVHRVVTNPDKE 295

Query: 123 RISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKGQRPVDGLRI 180
           RISL  FC PE D +I+P E L++E  PRL+K VKNY+  YF+ YQ+G+RP++  +I
Sbjct: 296 RISLAAFCIPESDKDIEPFESLVNESTPRLYKKVKNYVGIYFEYYQQGRRPIEAAKI 350

[Close]

BLAST of CSPI01G17060 vs. TAIR10
Match: AT5G20400.1 (AT5G20400.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 211.1 bits (536), Expect = 5.8e-55
Identity = 96/177 (54.24%), Postives = 131/177 (74.01%), Query Frame = 1


Query: 3   IIETVLIAMARSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAITI 62
           +IE    AMARSL++E NSF D  G+  TL TRFN YPPC +P  V+G+K H+DGSAIT+
Sbjct: 174 VIEQFFKAMARSLELEENSFLDMYGESATLDTRFNMYPPCPSPDKVIGVKPHADGSAITL 233

Query: 63  LLLDKQVEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSERQ 122
           LL DK V GLQ +KD +WY+ P+  + D++LI +G+Q E+MSNGI+KS +HR VTN E++
Sbjct: 234 LLPDKDVGGLQFQKDGKWYKAPI--VPDTILINVGDQMEIMSNGIYKSPVHRVVTNREKE 293

Query: 123 RISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKGQRPVDGLRI 180
           RIS+  FC P  D EI+P+  L+ E RPRL+K+VK Y+E YF+ YQ+G+RP++   I
Sbjct: 294 RISVATFCIPGADKEIQPVNELVSEARPRLYKTVKKYVELYFKYYQQGRRPIEAALI 348

[Close]

BLAST of CSPI01G17060 vs. TAIR10
Match: AT1G49390.1 (AT1G49390.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 197.2 bits (500), Expect = 8.6e-51
Identity = 88/177 (49.72%), Postives = 127/177 (71.75%), Query Frame = 1


Query: 3   IIETVLIAMARSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAITI 62
           +IE    AMARSL++E N F +  G+   + +RFNF+PPC  P  V+G+K H+DGSAIT+
Sbjct: 174 LIEKFFKAMARSLELEENCFLEMYGENAVMNSRFNFFPPCPRPDKVIGIKPHADGSAITL 233

Query: 63  LLLDKQVEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSERQ 122
           LL DK VEGLQ  KD +WY+ P+  + D++LI +G+Q E+MSNGI+KS +HR VTN E++
Sbjct: 234 LLPDKDVEGLQFLKDGKWYKAPI--VPDTILITLGDQMEIMSNGIYKSPVHRVVTNREKE 293

Query: 123 RISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKGQRPVDGLRI 180
           RIS+  FC P  D EI P +GL+ E RPRL+K+V  Y++ +++ YQ+G+R ++   I
Sbjct: 294 RISVATFCVPGLDKEIHPADGLVTEARPRLYKTVTKYVDLHYKYYQQGRRTIEAALI 348

[Close]

BLAST of CSPI01G17060 vs. TAIR10
Match: AT5G20550.1 (AT5G20550.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 195.7 bits (496), Expect = 2.5e-50
Identity = 90/172 (52.33%), Postives = 124/172 (72.09%), Query Frame = 1


Query: 3   IIETVLIAMARSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAITI 62
           +   V  AMA SL++E N F D  G+  T+ TRFN YPPC  P  V+G++ H+D SA T+
Sbjct: 174 VFNQVFKAMAISLELEENCFLDMCGENATMDTRFNMYPPCPRPDKVIGVRPHADKSAFTL 233

Query: 63  LLLDKQVEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSERQ 122
           LL DK VEGLQ  KD +WY+ PV A +D++LI +G+Q E+MSNGI+KS +HR VTN+E++
Sbjct: 234 LLPDKNVEGLQFLKDGKWYKAPVVA-SDTILINVGDQMEIMSNGIYKSPVHRVVTNTEKE 293

Query: 123 RISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKGQRPV 175
           RIS+  FC P  D EI+P++GL+ E RPRL+K VKNY++   + Y +GQRP+
Sbjct: 294 RISVATFCIPGADKEIQPVDGLVSEARPRLYKPVKNYVDLLNKYYIQGQRPI 344

[Close]

BLAST of CSPI01G17060 vs. TAIR10
Match: AT5G54000.1 (AT5G54000.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 187.2 bits (474), Expect = 8.9e-48
Identity = 86/177 (48.59%), Postives = 122/177 (68.93%), Query Frame = 1


Query: 3   IIETVLIAMARSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAITI 62
           ++E    A+ARSL++E N F +  G+  TL TRFN YPPC  P  VLGLK HSDGSA T+
Sbjct: 175 VVEKFFKALARSLELEDNCFLEMHGENATLETRFNIYPPCPRPDKVLGLKPHSDGSAFTL 234

Query: 63  LLLDKQVEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSERQ 122
           +L DK VEGLQ  KD +WY+  +  +  ++LI +G+  EVMSNGI+KS +HR V N +++
Sbjct: 235 ILPDKNVEGLQFLKDGKWYKASI--LPHTILINVGDTMEVMSNGIYKSPVHRVVLNGKKE 294

Query: 123 RISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKGQRPVDGLRI 180
           RI +  FC  ++D EI+P+ GL+ E RPRL+K+VK   + +F  YQ+G+RP++   I
Sbjct: 295 RIYVATFCNADEDKEIQPLNGLVSEARPRLYKAVKKSEKNFFDYYQQGRRPIEAAMI 349

[Close]

BLAST of CSPI01G17060 vs. TAIR10
Match: AT3G21420.1 (AT3G21420.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 132.9 bits (333), Expect = 2.0e-31
Identity = 74/182 (40.66%), Postives = 114/182 (62.64%), Query Frame = 1


Query: 2   EIIETVLIAMARSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAIT 61
           E+ + +L  +A SL ++   F +  G+    + R N+YPPCS+P LVLGL  HSDGSA+T
Sbjct: 184 ELCKRLLKYIAISLGLKEERFEEMFGEAVQAV-RMNYYPPCSSPDLVLGLSPHSDGSALT 243

Query: 62  ILLLDKQ-VEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSE 121
           +L   K    GLQ+ KD+ W  VPV  + ++L+I IG+  EV+SNG +KS  HRAVTN E
Sbjct: 244 VLQQSKNSCVGLQILKDNTW--VPVKPLPNALVINIGDTIEVLSNGKYKSVEHRAVTNRE 303

Query: 122 RQRISLVCFCCPEKDIEIKPIEGLI-DEKRPRLFKSVK--NYLETYFQNYQKGQRPVDGL 180
           ++R+++V F  P  ++EI+P+  L+ DE  P  ++S    +Y   Y  N  +G++ +D  
Sbjct: 304 KERLTIVTFYAPNYEVEIEPMSELVDDETNPCKYRSYNHGDYSYHYVSNKLQGKKSLDFA 362

[Close]

BLAST of CSPI01G17060 vs. NCBI nr
Match: gi|778660153|ref|XP_011655698.1| (PREDICTED: protein SRG1-like [Cucumis sativus])

HSP 1 Score: 360.5 bits (924), Expect = 1.7e-96
Identity = 175/179 (97.77%), Postives = 178/179 (99.44%), Query Frame = 1


Query: 1   MEIIETVLIAMARSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAI 60
           MEIIETVLIAMA SLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAI
Sbjct: 176 MEIIETVLIAMASSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAI 235

Query: 61  TILLLDKQVEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSE 120
           TILLLDKQVEGLQLRKDDQWYRVPVPAIADSLL++IGEQAEVMSNGIFKSSIHRAVTNSE
Sbjct: 236 TILLLDKQVEGLQLRKDDQWYRVPVPAIADSLLLVIGEQAEVMSNGIFKSSIHRAVTNSE 295

Query: 121 RQRISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKGQRPVDGLRI 180
           RQRISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKG+RPVDGLRI
Sbjct: 296 RQRISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKGRRPVDGLRI 354

[Close]

BLAST of CSPI01G17060 vs. NCBI nr
Match: gi|700210098|gb|KGN65194.1| (hypothetical protein Csa_1G263970 [Cucumis sativus])

HSP 1 Score: 360.5 bits (924), Expect = 1.7e-96
Identity = 175/179 (97.77%), Postives = 178/179 (99.44%), Query Frame = 1


Query: 1   MEIIETVLIAMARSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAI 60
           MEIIETVLIAMA SLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAI
Sbjct: 20  MEIIETVLIAMASSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAI 79

Query: 61  TILLLDKQVEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSE 120
           TILLLDKQVEGLQLRKDDQWYRVPVPAIADSLL++IGEQAEVMSNGIFKSSIHRAVTNSE
Sbjct: 80  TILLLDKQVEGLQLRKDDQWYRVPVPAIADSLLLVIGEQAEVMSNGIFKSSIHRAVTNSE 139

Query: 121 RQRISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKGQRPVDGLRI 180
           RQRISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKG+RPVDGLRI
Sbjct: 140 RQRISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKGRRPVDGLRI 198

[Close]

BLAST of CSPI01G17060 vs. NCBI nr
Match: gi|778659909|ref|XP_011655280.1| (PREDICTED: protein SRG1-like [Cucumis sativus])

HSP 1 Score: 351.7 bits (901), Expect = 7.7e-94
Identity = 168/179 (93.85%), Postives = 177/179 (98.88%), Query Frame = 1


Query: 1   MEIIETVLIAMARSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAI 60
           MEIIETVLIAMARSL+VEPNSFTDQVG+RPTL TRFNFYPPCSTPHLVLGLKEHSDGSAI
Sbjct: 176 MEIIETVLIAMARSLNVEPNSFTDQVGERPTLFTRFNFYPPCSTPHLVLGLKEHSDGSAI 235

Query: 61  TILLLDKQVEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSE 120
           TILLLDKQVEGLQLRKDDQWYRVPVPAIADSLL++IGEQAEVMSNGIFKSS+HRAVTNSE
Sbjct: 236 TILLLDKQVEGLQLRKDDQWYRVPVPAIADSLLLVIGEQAEVMSNGIFKSSVHRAVTNSE 295

Query: 121 RQRISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKGQRPVDGLRI 180
           RQRIS+VCFCCPEKDIEIKP+EGLIDEKRPRLF+SVKNYLETYFQNYQ+GQR VDGLRI
Sbjct: 296 RQRISVVCFCCPEKDIEIKPVEGLIDEKRPRLFRSVKNYLETYFQNYQEGQRTVDGLRI 354

[Close]

BLAST of CSPI01G17060 vs. NCBI nr
Match: gi|700210001|gb|KGN65097.1| (hypothetical protein Csa_1G212830 [Cucumis sativus])

HSP 1 Score: 351.7 bits (901), Expect = 7.7e-94
Identity = 168/179 (93.85%), Postives = 177/179 (98.88%), Query Frame = 1


Query: 1   MEIIETVLIAMARSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAI 60
           MEIIETVLIAMARSL+VEPNSFTDQVG+RPTL TRFNFYPPCSTPHLVLGLKEHSDGSAI
Sbjct: 399 MEIIETVLIAMARSLNVEPNSFTDQVGERPTLFTRFNFYPPCSTPHLVLGLKEHSDGSAI 458

Query: 61  TILLLDKQVEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSE 120
           TILLLDKQVEGLQLRKDDQWYRVPVPAIADSLL++IGEQAEVMSNGIFKSS+HRAVTNSE
Sbjct: 459 TILLLDKQVEGLQLRKDDQWYRVPVPAIADSLLLVIGEQAEVMSNGIFKSSVHRAVTNSE 518

Query: 121 RQRISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKGQRPVDGLRI 180
           RQRIS+VCFCCPEKDIEIKP+EGLIDEKRPRLF+SVKNYLETYFQNYQ+GQR VDGLRI
Sbjct: 519 RQRISVVCFCCPEKDIEIKPVEGLIDEKRPRLFRSVKNYLETYFQNYQEGQRTVDGLRI 577

[Close]

BLAST of CSPI01G17060 vs. NCBI nr
Match: gi|659131148|ref|XP_008465534.1| (PREDICTED: protein SRG1-like isoform X2 [Cucumis melo])

HSP 1 Score: 338.2 bits (866), Expect = 8.9e-90
Identity = 161/179 (89.94%), Postives = 173/179 (96.65%), Query Frame = 1


Query: 1   MEIIETVLIAMARSLDVEPNSFTDQVGKRPTLLTRFNFYPPCSTPHLVLGLKEHSDGSAI 60
           M+II+TVLIAMARSL+VEPNSFTDQVGKRPTL  RFNFYPPCSTPHLVLGLKEHSDG+AI
Sbjct: 176 MKIIDTVLIAMARSLNVEPNSFTDQVGKRPTLFKRFNFYPPCSTPHLVLGLKEHSDGTAI 235

Query: 61  TILLLDKQVEGLQLRKDDQWYRVPVPAIADSLLIIIGEQAEVMSNGIFKSSIHRAVTNSE 120
           T+LLLDKQVEGL+LRKDDQWYRVPVPA+ADSLLIIIGEQAE+MSNGIFKS IHRAVTNSE
Sbjct: 236 TVLLLDKQVEGLELRKDDQWYRVPVPAVADSLLIIIGEQAEIMSNGIFKSLIHRAVTNSE 295

Query: 121 RQRISLVCFCCPEKDIEIKPIEGLIDEKRPRLFKSVKNYLETYFQNYQKGQRPVDGLRI 180
           RQRIS+V FCCPEKDIEIKPIEGLIDEKRPRLF+S KNYL+TYFQNYQKG+R VDGLRI
Sbjct: 296 RQRISVVSFCCPEKDIEIKPIEGLIDEKRPRLFRSTKNYLQTYFQNYQKGERSVDGLRI 354

The following BLAST results are available for this feature:

BLAST of CSPI01G17060 vs. Swiss-Prot


Match Name	E-value	Identity	Description
DIOX2_PAPSO	5.3e-26	35.06	Probable 2-oxoglutarate/Fe(II)-dependent dioxygenase OS=Papaver somniferum GN=DI...	[more]
FLS_EUSER	5.3e-26	41.03	Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellian...	[more]
SRG1_ARATH	9.0e-26	34.59	Protein SRG1 OS=Arabidopsis thaliana GN=SRG1 PE=2 SV=1	[more]
DIOX3_PAPSO	1.5e-25	45.00	Codeine O-demethylase OS=Papaver somniferum GN=CODM PE=1 SV=1	[more]
DLO1_ARATH	1.1e-23	40.41	Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana GN=DLO1 PE=1 SV=1	[more]

BLAST of CSPI01G17060 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LW33_CUCSA	1.2e-96	97.77	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G263970 PE=4 SV=1	[more]
A0A0A0LW96_CUCSA	5.4e-94	93.85	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G212830 PE=3 SV=1	[more]
U7E2D8_POPTR	1.1e-54	56.50	Oxidoreductase family protein OS=Populus trichocarpa GN=POPTR_0025s00440g PE=3 S...	[more]
B9N703_POPTR	1.4e-54	57.30	Oxidoreductase family protein OS=Populus trichocarpa GN=POPTR_0006s06130g PE=3 S...	[more]
M5WFY4_PRUPE	5.5e-54	55.37	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007960mg PE=3 SV=1	[more]

BLAST of CSPI01G17060 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G20400.1	5.8e-55	54.24	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT1G49390.1	8.6e-51	49.72	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT5G20550.1	2.5e-50	52.33	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT5G54000.1	8.9e-48	48.59	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT3G21420.1	2.0e-31	40.66	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]

BLAST of CSPI01G17060 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778660153\|ref\|XP_011655698.1\|	1.7e-96	97.77	PREDICTED: protein SRG1-like [Cucumis sativus]	[more]
gi\|700210098\|gb\|KGN65194.1\|	1.7e-96	97.77	hypothetical protein Csa_1G263970 [Cucumis sativus]	[more]
gi\|778659909\|ref\|XP_011655280.1\|	7.7e-94	93.85	PREDICTED: protein SRG1-like [Cucumis sativus]	[more]
gi\|700210001\|gb\|KGN65097.1\|	7.7e-94	93.85	hypothetical protein Csa_1G212830 [Cucumis sativus]	[more]
gi\|659131148\|ref\|XP_008465534.1\|	8.9e-90	89.94	PREDICTED: protein SRG1-like isoform X2 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR005123	Oxoglu/Fe-dep_dioxygenase
IPR027443	IPNS-like

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G17060.1	CSPI01G17060.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 34..132 score: 5.9
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROFILE	PS51471	FE2OG_OXY	coord: 30..132 score: 11
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 2..174 score: 1.1
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 2..173 score: 5.6
None	No IPR available	PANTHER	PTHR10209:SF107	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE-LIKE PROTEIN-RELATED	coord: 2..173 score: 5.6
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 2..162 score: 1.1

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI01G17060	Wax gourd	cpiwgoB099
CSPI01G17060	Cucumber (Gy14) v1	cgycpiB518
CSPI01G17060	Cucurbita maxima (Rimu)	cmacpiB059
CSPI01G17060	Cucurbita maxima (Rimu)	cmacpiB097
CSPI01G17060	Cucurbita moschata (Rifu)	cmocpiB051
CSPI01G17060	Cucurbita moschata (Rifu)	cmocpiB090
CSPI01G17060	Cucumber (Chinese Long) v2	cpicuB002
CSPI01G17060	Melon (DHL92) v3.5.1	cpimeB057
CSPI01G17060	Watermelon (Charleston Gray)	cpiwcgB071
CSPI01G17060	Watermelon (97103) v1	cpiwmB086
CSPI01G17060	Cucurbita pepo (Zucchini)	cpecpiB351
CSPI01G17060	Cucurbita pepo (Zucchini)	cpecpiB640
CSPI01G17060	Melon (DHL92) v3.6.1	cpimedB051
CSPI01G17060	Cucumber (Gy14) v2	cgybcpiB002
CSPI01G17060	Silver-seed gourd	carcpiB0138
CSPI01G17060	Silver-seed gourd	carcpiB0608
CSPI01G17060	Cucumber (Chinese Long) v3	cpicucB000
CSPI01G17060	Watermelon (97103) v2	cpiwmbB068