CSPI01G17050 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI01G17050
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	2OG-Fe(II) oxygenase family oxidoreductase
Location	Chr1 : 12847722 .. 12849615 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCACAAAATTCTCAGAACACACAAACACAAACACTCCAACAACAACTTCTCATCAACGGCGGCCACACGCCGGAAAGTTATATTTACAAAGGCGGCTACCACGGCGGAGGTTCCAACAATAATACTCCACTTCCACTGGCAGAGATTCCGGTCGTTGACCTCTCTCAACTTTCATTGCCGTCTGCCGGCGAGGGTCCGTTGAACGACCTCCGTTTGGCTCTTAGTACTTGGGGATGCTTTCAGGTGAGCTTTCATGAACCTCAACACTGCCCTTTTCATATCAACTTTATTTTTAATTCTATTTTCTTCTAATTTTCTGCTTCAAACTTTTAATTTTACATTTCGATTTTTTTTAAAAATATATTTGAGTTTTTAGCTAAATTTTGAGAGGAAAGATTAGTAAATGGAGACAATTATTTTTAAGTTTCAAAACTTATTATTAATATTATGTTTGTGGTTTTTAAAAGATGGGATAATATAAAGAAATACATTATTAGATATACTTTTGTTTATTTGCTTGTGTCCAACATTATTGATGACACATATCTGTTTGTTTTTTTGTATGTTAATTCTATATATTTTTAGACAAAAGTATTGAATGATCAACAATATACAATTGCATAACACTTTTTTCATATTATAAACGTATGACTAGTATAGAGGGTAATCAGATATTAATTTACTTTTACATTTTTTATTTTTACAATTAAAAAAATATAGTGACATATACTCTATTATTATAAATGTTTTTTTTGCAAACTCCCTACTTTTTTGGTTGTATTTACAAATCGATTATGGTACAATTAGTCTTACCTATACTTATGTAACATCAAGAATATATATTTATATATATATATATATATGGAATGTTTGGCAACTAATTATATCTCCATTTTCTAACCATTTGGATTTTTTATTTTTATTTTTACAATTAAATCATTTTCTTGTAATTATTTAAATCTTTCTTGAATTTTTAGCAAAATTCAAAAATAAAATAATATTGACAATAATAAGGAATCCTTTGGTTTTCAAATTTTTATTTGATTTTTTAAAAATATTAATTTAAAACGTAGATTACAGTACAAACTATTTAAACATGGAAATAGTATTCATGTGCTTAATTAAAAATTAAAAATTAAAAAAAAAAAAACTTAAAAAGAAAAACCAACTATCCCAATATTTCCAAAAATGAGCTCTAATTCGAAATAGTAAGTTATATTAAGAATTCCTTTTTTTTTTCAAGAAATGAGAAACCATAGTATGTAGATATGAATTATAGTTTTTTCTTAAAAAATTGAAATAAAATAGTAATGGCTAAATGGTCATGAAACGGAGCAAAAGGGAAAGAGGAGTGATTGTTGTAAATTAATTTAGATTAACTAATTTGAGAGTGTGGTTTCAGGCTATTAATCACAGCATTTCAAGTTCATTTTTGGAGAAGATGAGAAAAATAAGTGAGCAATTTTTCTCATTGCCCATTGAAGAGAAGATGAGATATGGAAGAGAAGTTGATGGAATGGAAGGATATGGGAATGATTTAATTCTTTCAGAACAACAAATTCTAGATTGGTCTGATCGTTTATATTTCGTGACAAATCCTGAAGATGAAAGACGTCTCGAGCTTTGGCCTTTAAATCCTCCATCTTTCAGGTTAGTTTCAACGCTAATCTTTCATATATCTAAATCAGTTCAACATTCTCAAAAATTGTCACTACAAGAATGAAAACCTTTCTCGACGCAAAAATACGTCTAAAAAAACACAAAATGCGTCGGAGAAAGTATTTCCAACATCCATATGCAACGTCGGCTTGAACGTCGGAGAAACAACGTTGGGGACACCTTCCCCGGCACGTGCGACGCGAACGCCAGTGTAATTTTTACCGATGCATAA

mRNA sequence

ATGGCACAAAATTCTCAGAACACACAAACACAAACACTCCAACAACAACTTCTCATCAACGGCGGCCACACGCCGGAAAGTTATATTTACAAAGGCGGCTACCACGGCGGAGGTTCCAACAATAATACTCCACTTCCACTGGCAGAGATTCCGGTCGTTGACCTCTCTCAACTTTCATTGCCGTCTGCCGGCGAGGGTCCGTTGAACGACCTCCGTTTGGCTCTTAGTACTTGGGGATGCTTTCAGGCTATTAATCACAGCATTTCAAGTTCATTTTTGGAGAAGATGAGAAAAATAAGTGAGCAATTTTTCTCATTGCCCATTGAAGAGAAGATGAGATATGGAAGAGAAGTTGATGGAATGGAAGGATATGGGAATGATTTAATTCTTTCAGAACAACAAATTCTAGATTGGTCTGATCGTTTATATTTCGTGACAAATCCTGAAGATGAAAGACGTCTCGAGCTTTGGCCTTTAAATCCTCCATCTTTCAGAAACAACGTTGGGGACACCTTCCCCGGCACGTGCGACGCGAACGCCAGTGTAATTTTTACCGATGCATAA

Coding sequence (CDS)

ATGGCACAAAATTCTCAGAACACACAAACACAAACACTCCAACAACAACTTCTCATCAACGGCGGCCACACGCCGGAAAGTTATATTTACAAAGGCGGCTACCACGGCGGAGGTTCCAACAATAATACTCCACTTCCACTGGCAGAGATTCCGGTCGTTGACCTCTCTCAACTTTCATTGCCGTCTGCCGGCGAGGGTCCGTTGAACGACCTCCGTTTGGCTCTTAGTACTTGGGGATGCTTTCAGGCTATTAATCACAGCATTTCAAGTTCATTTTTGGAGAAGATGAGAAAAATAAGTGAGCAATTTTTCTCATTGCCCATTGAAGAGAAGATGAGATATGGAAGAGAAGTTGATGGAATGGAAGGATATGGGAATGATTTAATTCTTTCAGAACAACAAATTCTAGATTGGTCTGATCGTTTATATTTCGTGACAAATCCTGAAGATGAAAGACGTCTCGAGCTTTGGCCTTTAAATCCTCCATCTTTCAGAAACAACGTTGGGGACACCTTCCCCGGCACGTGCGACGCGAACGCCAGTGTAATTTTTACCGATGCATAA

Homology

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BLAST of CSPI01G17050 vs. Swiss-Prot
Match: NCS1_COPJA (S-norcoclaurine synthase 1 OS=Coptis japonica GN=NCS1 PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.7e-22
Identity = 51/120 (42.50%), Postives = 68/120 (56.67%), Query Frame = 1


Query: 49  EIPVVDLSQLSLPSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPI 108
           EIPV+DLS+L         L     A   WG FQ INH +    +EKM+  +E FF LP 
Sbjct: 50  EIPVIDLSRLLDQQYACDELAKFHSACLDWGFFQLINHGVREEVIEKMKVDTEDFFRLPF 109

Query: 109 EEKMRYGREVDGMEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNV 168
           +EK  Y +  +GMEGYG   + SE+Q LDW+D  + +T P  ER +  WP +P SFR  +
Sbjct: 110 KEKNAYRQLPNGMEGYGQAFVTSEEQKLDWADMHFLITKPVQERNMRFWPTSPTSFRETM 169

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BLAST of CSPI01G17050 vs. Swiss-Prot
Match: SRG1_ARATH (Protein SRG1 OS=Arabidopsis thaliana GN=SRG1 PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.7e-20
Identity = 44/120 (36.67%), Postives = 72/120 (60.00%), Query Frame = 1


Query: 49  EIPVVDLSQLSLPSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPI 108
           EIP++D+ +L   +  +  +  L  A   WG FQ +NH I SSFL+K++   + FF+LP+
Sbjct: 52  EIPIIDMKRLCSSTTMDSEVEKLDFACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPM 111

Query: 109 EEKMRYGREVDGMEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNV 168
           EEK ++ +  D +EG+G   ++SE Q LDW+D  +    P + R+  L+P  P  FR+ +
Sbjct: 112 EEKKKFWQRPDEIEGFGQAFVVSEDQKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTL 171

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BLAST of CSPI01G17050 vs. Swiss-Prot
Match: DIOX3_PAPSO (Codeine O-demethylase OS=Papaver somniferum GN=CODM PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 7.5e-15
Identity = 41/121 (33.88%), Postives = 66/121 (54.55%), Query Frame = 1


Query: 50  IPVVDLSQLSLPS--AGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLP 109
           +PV+DL  L  P    G+  L+ L  A   WG FQ +NH + +  ++ ++   + FF+LP
Sbjct: 54  VPVIDLQNLLSPEPVVGKLELDKLHSACKEWGFFQLVNHGVDALLMDNIKSEIKGFFNLP 113

Query: 110 IEEKMRYGREVDGMEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFRNN 169
           + EK +YG++    EG+G   I SE Q LDW++    ++ P   R+  L+P  P  FR  
Sbjct: 114 MNEKTKYGQQDGDFEGFGQPYIESEDQRLDWTEVFSMLSLPLHLRKPHLFPELPLPFRET 173

[Close]

BLAST of CSPI01G17050 vs. Swiss-Prot
Match: LDOX_ARATH (Leucoanthocyanidin dioxygenase OS=Arabidopsis thaliana GN=LDOX PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 9.8e-15
Identity = 37/120 (30.83%), Postives = 64/120 (53.33%), Query Frame = 1


Query: 49  EIPVVDLSQLSLPSAG--EGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSL 108
           ++P +DL  +        E  + +L+ A   WG    INH I +  +E+++K  E+FFSL
Sbjct: 46  QVPTIDLKNIESDDEKIRENCIEELKKASLDWGVMHLINHGIPADLMERVKKAGEEFFSL 105

Query: 109 PIEEKMRYGRE--VDGMEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSF 165
            +EEK +Y  +     ++GYG+ L  +    L+W D  + +  PE++R L +WP  P  +
Sbjct: 106 SVEEKEKYANDQATGKIQGYGSKLANNASGQLEWEDYFFHLAYPEEKRDLSIWPKTPSDY 165

[Close]

BLAST of CSPI01G17050 vs. Swiss-Prot
Match: DIOX1_PAPSO (Thebaine 6-O-demethylase OS=Papaver somniferum GN=T6ODM PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.3e-14
Identity = 45/140 (32.14%), Postives = 70/140 (50.00%), Query Frame = 1


Query: 39  SNNNTPLPLAE--------IPVVDLSQLSLPSA--GEGPLNDLRLALSTWGCFQAINHSI 98
           +N N  LP+          IPV+D+  L  P    G+  L+ L  A   WG FQ +NH +
Sbjct: 36  ANENLLLPMGASVINDHETIPVIDIENLLSPEPIIGKLELDRLHFACKEWGFFQVVNHGV 95

Query: 99  SSSFLEKMRKISEQFFSLPIEEKMRYGREVDGMEGYGNDLILSEQQILDWSDRLYFVTNP 158
            +S ++ ++   + FF+L ++EK +Y +E   +EG+G   I SE Q LDW+D     T P
Sbjct: 96  DASLVDSVKSEIQGFFNLSMDEKTKYEQEDGDVEGFGQGFIESEDQTLDWADIFMMFTLP 155

Query: 159 EDERRLELWPLNPPSFRNNV 169
              R+  L+   P   R  +
Sbjct: 156 LHLRKPHLFSKLPVPLRETI 175

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BLAST of CSPI01G17050 vs. TrEMBL
Match: A0A0A0LWH8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G263960 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 8.7e-87
Identity = 159/164 (96.95%), Postives = 159/164 (96.95%), Query Frame = 1


Query: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSL 60
           MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLS 
Sbjct: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSS 60

Query: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120
           PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG
Sbjct: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 MEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSF 165
           MEGYGNDL  S QQ LDWSDRLYFVTNPEDERRLELWPLNPPSF
Sbjct: 121 MEGYGNDLTFSNQQTLDWSDRLYFVTNPEDERRLELWPLNPPSF 164

[Close]

BLAST of CSPI01G17050 vs. TrEMBL
Match: A0A0A0LW96_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G212830 PE=3 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 4.5e-51
Identity = 98/111 (88.29%), Postives = 103/111 (92.79%), Query Frame = 1


Query: 58  LSLPSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGRE 117
           L  PSAGEGPLNDLRLALSTWGCFQA NHSISSSFLEK+RKISEQFFSLPIEEKMRYGRE
Sbjct: 281 LKSPSAGEGPLNDLRLALSTWGCFQATNHSISSSFLEKLRKISEQFFSLPIEEKMRYGRE 340

Query: 118 VDGMEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNV 169
           VDGMEGYGNDL  S QQ LDWSDRLYFVT+PEDERRL+LWPLNPPSFR ++
Sbjct: 341 VDGMEGYGNDLTFSNQQTLDWSDRLYFVTSPEDERRLDLWPLNPPSFREDL 391

[Close]

BLAST of CSPI01G17050 vs. TrEMBL
Match: A0A067JH25_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_00345 PE=3 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 8.0e-40
Identity = 81/151 (53.64%), Postives = 106/151 (70.20%), Query Frame = 1


Query: 15  QQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSLPSAGEGPLNDLRLA 74
           Q+L I+G  + E YI KG   G     NT + L E+P+VD+  L  P      L+ LR A
Sbjct: 14  QELAIDGEESLEKYIQKGTDSG---KCNTSVSLIEVPIVDIGLLKSPLTRTEELHKLRSA 73

Query: 75  LSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDGMEGYGNDLILSEQQ 134
           LS+WGCFQ INH ++SSFLE+MR++++QFFSLP+EEK +Y R+   MEGYGND++L E Q
Sbjct: 74  LSSWGCFQVINHGMTSSFLEEMRQVAKQFFSLPMEEKQKYSRKTGSMEGYGNDMVLLEHQ 133

Query: 135 ILDWSDRLYFVTNPEDERRLELWPLNPPSFR 166
           ILDW+DRLY   NPED+R+L  WP NP +FR
Sbjct: 134 ILDWTDRLYLTLNPEDQRQLRFWPENPQTFR 161

[Close]

BLAST of CSPI01G17050 vs. TrEMBL
Match: A0A067KLK8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_10453 PE=4 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.8e-39
Identity = 80/151 (52.98%), Postives = 106/151 (70.20%), Query Frame = 1


Query: 15  QQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSLPSAGEGPLNDLRLA 74
           Q+L I+G  + E YI KG   G     NT +PL E+P+VD+  L  P      L+ LR A
Sbjct: 13  QELAIDGKESLEKYIQKGTDSG---KLNTSVPLIEVPIVDIGLLKSPLTRTEELHKLRSA 72

Query: 75  LSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDGMEGYGNDLILSEQQ 134
           LS+WGCFQ INH ++SSFLE+MR+++ QFFSLP+EEK +Y R+   +EGYGND++L E Q
Sbjct: 73  LSSWGCFQVINHGMTSSFLEEMRQVATQFFSLPMEEKQKYSRKTGSIEGYGNDMVLLEHQ 132

Query: 135 ILDWSDRLYFVTNPEDERRLELWPLNPPSFR 166
           ILDW+DRLY   NPE++R+L  WP NP +FR
Sbjct: 133 ILDWTDRLYLTLNPENQRQLRFWPENPQTFR 160

[Close]

BLAST of CSPI01G17050 vs. TrEMBL
Match: U7E1C4_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0025s00430g PE=4 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.3e-39
Identity = 78/154 (50.65%), Postives = 105/154 (68.18%), Query Frame = 1


Query: 15  QQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSLPSAGEGPLNDLRLA 74
           Q++  NG   P  Y  KG   G     + P+PL EIPVVDL  L+ P      L  L+LA
Sbjct: 13  QEMATNGQEPPVKYFSKGNDAGV---LDAPVPLIEIPVVDLGLLTSPLTSAQELEKLKLA 72

Query: 75  LSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDGMEGYGNDLILSEQQ 134
           LS+WGCFQ INH ++SSFL+K+R++S+QFF  P+EEK +Y RE D +EGYGND+ILS+ Q
Sbjct: 73  LSSWGCFQVINHGMTSSFLDKIREVSKQFFGFPMEEKQKYSREADSIEGYGNDMILSDHQ 132

Query: 135 ILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNV 169
            +DW+DRLY   +PED+R+++ WP NP  FR  +
Sbjct: 133 TVDWTDRLYLTISPEDQRKIKFWPENPKDFRETL 163

[Close]

BLAST of CSPI01G17050 vs. TAIR10
Match: AT5G20550.1 (AT5G20550.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 142.5 bits (358), Expect = 2.6e-34
Identity = 68/155 (43.87%), Postives = 98/155 (63.23%), Query Frame = 1


Query: 15  QQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSLPSA-GEGPLNDLRL 74
           Q+++  G   PE Y+        G + N  +P+ +IP +DLS L  PS  G   L+ L  
Sbjct: 10  QEVVAAGEGIPERYLQPPAVDDNGQHLNAAVPVMDIPAIDLSLLLSPSDDGREELSKLHS 69

Query: 75  ALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDGMEGYGNDLILSEQ 134
           ALSTWG  Q INH I+ + L+K+ K++++F +LP EEK +Y RE+  ++GYGND+IL + 
Sbjct: 70  ALSTWGVVQVINHGITKALLDKIYKLTKEFCALPSEEKQKYAREIGSIQGYGNDMILWDD 129

Query: 135 QILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNV 169
           Q+LDW DRLY  T PED+R+L+ WP  P  FR  +
Sbjct: 130 QVLDWIDRLYITTYPEDQRQLKFWPDVPVGFRETL 164

[Close]

BLAST of CSPI01G17050 vs. TAIR10
Match: AT5G20400.1 (AT5G20400.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 141.0 bits (354), Expect = 7.7e-34
Identity = 68/155 (43.87%), Postives = 99/155 (63.87%), Query Frame = 1


Query: 15  QQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQL-SLPSAGEGPLNDLRL 74
           Q+++  G   PE Y++     G     N  +P  +IP +DL+ L S   AG+  L+ L  
Sbjct: 10  QEVVAAGEGLPERYLHAPTGDGEVQPLNAAVPEMDIPAIDLNLLLSSSEAGQQELSKLHS 69

Query: 75  ALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDGMEGYGNDLILSEQ 134
           ALSTWG  Q +NH I+ +FL+K+ K++++FF+LP EEK +  RE+D ++GYGND+IL + 
Sbjct: 70  ALSTWGVVQVMNHGITKAFLDKIYKLTKEFFALPTEEKQKCAREIDSIQGYGNDMILWDD 129

Query: 135 QILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNV 169
           Q+LDW DRLY  T PED+R+L  WP  P  FR  +
Sbjct: 130 QVLDWIDRLYITTYPEDQRQLNFWPEVPLGFRETL 164

[Close]

BLAST of CSPI01G17050 vs. TAIR10
Match: AT5G54000.1 (AT5G54000.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 141.0 bits (354), Expect = 7.7e-34
Identity = 68/156 (43.59%), Postives = 99/156 (63.46%), Query Frame = 1


Query: 15  QQLLINGGHTPESYIYKGGYHGGGSNN-NTPLPLAEIPVVDLSQL-SLPSAGEGPLNDLR 74
           Q+++  G   PE Y+Y     G G    N  LP  +I ++DL+ L S    G   L+ L 
Sbjct: 10  QEVVAAGEKLPERYLYTPTGDGEGDQPFNGLLPEMKISIIDLNLLFSSSDDGREELSKLH 69

Query: 75  LALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDGMEGYGNDLILSE 134
            A+STWG  Q +NH IS + L+K+ ++++QFF LP +EK +Y RE+   +G+GND+ILS+
Sbjct: 70  SAISTWGVVQVMNHGISEALLDKIHELTKQFFVLPTKEKQKYAREISSFQGFGNDMILSD 129

Query: 135 QQILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNV 169
            Q+LDW DRLY +T PED+R+L+ WP NP  FR  +
Sbjct: 130 DQVLDWVDRLYLITYPEDQRQLKFWPENPSGFRETL 165

[Close]

BLAST of CSPI01G17050 vs. TAIR10
Match: AT1G49390.1 (AT1G49390.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 136.0 bits (341), Expect = 2.5e-32
Identity = 66/157 (42.04%), Postives = 97/157 (61.78%), Query Frame = 1


Query: 15  QQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSLPSA-GEGPLNDLRL 74
           Q+++  G   PE Y++     G     N  +P  +IP +DLS L   S  G+  +  L  
Sbjct: 10  QEVVAAGQGLPERYLHAPTGEGESQPLNGAVPEMDIPAIDLSLLFSSSVDGQEEMKKLHS 69

Query: 75  ALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDGMEGYGNDLILSEQ 134
           ALSTWG  Q +NH I+ +FL+K+ K+++QFF+LP EEK +  RE   ++GYGND+ILS+ 
Sbjct: 70  ALSTWGVVQVMNHGITEAFLDKIYKLTKQFFALPTEEKHKCARETGNIQGYGNDMILSDN 129

Query: 135 QILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNVGD 171
           Q+LDW DRL+  T PED+R+L+ WP  P  F   + +
Sbjct: 130 QVLDWIDRLFLTTYPEDKRQLKFWPQVPVGFSETLDE 166

[Close]

BLAST of CSPI01G17050 vs. TAIR10
Match: AT1G78550.1 (AT1G78550.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 103.2 bits (256), Expect = 1.8e-22
Identity = 52/137 (37.96%), Postives = 77/137 (56.20%), Query Frame = 1


Query: 48  AEIPVVDLSQLSLPSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLP 107
           +EIPV+D+++L   SA +  L  L  A   WG FQ +NH I SSFLEK+    ++FF+LP
Sbjct: 51  SEIPVIDMTRLCSVSAMDSELKKLDFACQDWGFFQLVNHGIDSSFLEKLETEVQEFFNLP 110

Query: 108 IEEKMRYGREVDGMEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFRNN 167
           ++EK +  +     EG+G   I+SE Q LDW D     T P   R+  L+   PP FR  
Sbjct: 111 MKEKQKLWQRSGEFEGFGQVNIVSENQKLDWGDMFILTTEPIRSRKSHLFSKLPPPFRET 170

Query: 168 VGDTFPGTCDANASVIF 185
           + +T+     + A ++F
Sbjct: 171 L-ETYSSEVKSIAKILF 186

[Close]

BLAST of CSPI01G17050 vs. NCBI nr
Match: gi|778660153|ref|XP_011655698.1| (PREDICTED: protein SRG1-like [Cucumis sativus])

HSP 1 Score: 330.9 bits (847), Expect = 1.5e-87
Identity = 160/170 (94.12%), Postives = 163/170 (95.88%), Query Frame = 1


Query: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSL 60
           MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLS 
Sbjct: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSS 60

Query: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120
           PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG
Sbjct: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 MEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNVGD 171
           MEGYGNDL  S QQ LDWSDRLYFVTNPEDERRLELWPLNPPSFR ++ +
Sbjct: 121 MEGYGNDLTFSNQQTLDWSDRLYFVTNPEDERRLELWPLNPPSFREDLDE 170

[Close]

BLAST of CSPI01G17050 vs. NCBI nr
Match: gi|700210096|gb|KGN65192.1| (hypothetical protein Csa_1G263960 [Cucumis sativus])

HSP 1 Score: 327.8 bits (839), Expect = 1.3e-86
Identity = 159/164 (96.95%), Postives = 159/164 (96.95%), Query Frame = 1


Query: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSL 60
           MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLS 
Sbjct: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSS 60

Query: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120
           PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG
Sbjct: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 MEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSF 165
           MEGYGNDL  S QQ LDWSDRLYFVTNPEDERRLELWPLNPPSF
Sbjct: 121 MEGYGNDLTFSNQQTLDWSDRLYFVTNPEDERRLELWPLNPPSF 164

[Close]

BLAST of CSPI01G17050 vs. NCBI nr
Match: gi|778659909|ref|XP_011655280.1| (PREDICTED: protein SRG1-like [Cucumis sativus])

HSP 1 Score: 324.3 bits (830), Expect = 1.4e-85
Identity = 156/168 (92.86%), Postives = 161/168 (95.83%), Query Frame = 1


Query: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSL 60
           MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLS 
Sbjct: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSS 60

Query: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120
           PSAGEGPLNDLRLALSTWGCFQA NHSISSSFLEK+RKISEQFFSLPIEEKMRYGREVDG
Sbjct: 61  PSAGEGPLNDLRLALSTWGCFQATNHSISSSFLEKLRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 MEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNV 169
           MEGYGNDL  S QQ LDWSDRLYFVT+PEDERRL+LWPLNPPSFR ++
Sbjct: 121 MEGYGNDLTFSNQQTLDWSDRLYFVTSPEDERRLDLWPLNPPSFREDL 168

[Close]

BLAST of CSPI01G17050 vs. NCBI nr
Match: gi|659131148|ref|XP_008465534.1| (PREDICTED: protein SRG1-like isoform X2 [Cucumis melo])

HSP 1 Score: 305.1 bits (780), Expect = 8.7e-80
Identity = 148/168 (88.10%), Postives = 153/168 (91.07%), Query Frame = 1


Query: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSL 60
           MAQNSQ  QTQTLQQQLLINGGHTPESYIYKGGYHGG SNNNTPLPLA+IPV+DLSQLS 
Sbjct: 1   MAQNSQIPQTQTLQQQLLINGGHTPESYIYKGGYHGGDSNNNTPLPLAQIPVLDLSQLSS 60

Query: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120
            S GE PLN LRLALSTWGCFQA NH ISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG
Sbjct: 61  ASVGEAPLNHLRLALSTWGCFQATNHGISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 MEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFRNNV 169
           MEGYGNDLI S QQILDWSDRLY VTNP+DERRLE WPLNPPSFR ++
Sbjct: 121 MEGYGNDLIFSNQQILDWSDRLYLVTNPKDERRLEFWPLNPPSFREDL 168

[Close]

BLAST of CSPI01G17050 vs. NCBI nr
Match: gi|659131146|ref|XP_008465533.1| (PREDICTED: protein SRG1-like isoform X1 [Cucumis melo])

HSP 1 Score: 304.7 bits (779), Expect = 1.1e-79
Identity = 148/166 (89.16%), Postives = 152/166 (91.57%), Query Frame = 1


Query: 1   MAQNSQNTQTQTLQQQLLINGGHTPESYIYKGGYHGGGSNNNTPLPLAEIPVVDLSQLSL 60
           MAQNSQ  QTQTLQQQLLINGGHTPESYIYKGGYHGG SNNNTPLPLA+IPV+DLSQLS 
Sbjct: 1   MAQNSQIPQTQTLQQQLLINGGHTPESYIYKGGYHGGDSNNNTPLPLAQIPVLDLSQLSS 60

Query: 61  PSAGEGPLNDLRLALSTWGCFQAINHSISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120
            S GE PLN LRLALSTWGCFQA NH ISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG
Sbjct: 61  ASVGEAPLNHLRLALSTWGCFQATNHGISSSFLEKMRKISEQFFSLPIEEKMRYGREVDG 120

Query: 121 MEGYGNDLILSEQQILDWSDRLYFVTNPEDERRLELWPLNPPSFRN 167
           MEGYGNDLI S QQILDWSDRLY VTNP+DERRLE WPLNPPSFR+
Sbjct: 121 MEGYGNDLIFSNQQILDWSDRLYLVTNPKDERRLEFWPLNPPSFRS 166

The following BLAST results are available for this feature:

BLAST of CSPI01G17050 vs. Swiss-Prot


Match Name	E-value	Identity	Description
NCS1_COPJA	1.7e-22	42.50	S-norcoclaurine synthase 1 OS=Coptis japonica GN=NCS1 PE=1 SV=1	[more]
SRG1_ARATH	2.7e-20	36.67	Protein SRG1 OS=Arabidopsis thaliana GN=SRG1 PE=2 SV=1	[more]
DIOX3_PAPSO	7.5e-15	33.88	Codeine O-demethylase OS=Papaver somniferum GN=CODM PE=1 SV=1	[more]
LDOX_ARATH	9.8e-15	30.83	Leucoanthocyanidin dioxygenase OS=Arabidopsis thaliana GN=LDOX PE=1 SV=1	[more]
DIOX1_PAPSO	1.3e-14	32.14	Thebaine 6-O-demethylase OS=Papaver somniferum GN=T6ODM PE=1 SV=1	[more]

BLAST of CSPI01G17050 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LWH8_CUCSA	8.7e-87	96.95	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G263960 PE=4 SV=1	[more]
A0A0A0LW96_CUCSA	4.5e-51	88.29	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G212830 PE=3 SV=1	[more]
A0A067JH25_JATCU	8.0e-40	53.64	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_00345 PE=3 SV=1	[more]
A0A067KLK8_JATCU	1.8e-39	52.98	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_10453 PE=4 SV=1	[more]
U7E1C4_POPTR	2.3e-39	50.65	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0025s00430g PE=4 SV=1	[more]

BLAST of CSPI01G17050 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G20550.1	2.6e-34	43.87	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT5G20400.1	7.7e-34	43.87	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT5G54000.1	7.7e-34	43.59	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT1G49390.1	2.5e-32	42.04	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]
AT1G78550.1	1.8e-22	37.96	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot...	[more]

BLAST of CSPI01G17050 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778660153\|ref\|XP_011655698.1\|	1.5e-87	94.12	PREDICTED: protein SRG1-like [Cucumis sativus]	[more]
gi\|700210096\|gb\|KGN65192.1\|	1.3e-86	96.95	hypothetical protein Csa_1G263960 [Cucumis sativus]	[more]
gi\|778659909\|ref\|XP_011655280.1\|	1.4e-85	92.86	PREDICTED: protein SRG1-like [Cucumis sativus]	[more]
gi\|659131148\|ref\|XP_008465534.1\|	8.7e-80	88.10	PREDICTED: protein SRG1-like isoform X2 [Cucumis melo]	[more]
gi\|659131146\|ref\|XP_008465533.1\|	1.1e-79	89.16	PREDICTED: protein SRG1-like isoform X1 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR026992	DIOX_N
IPR027443	IPNS-like

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G17050.1	CSPI01G17050.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 50..158 score: 1.2
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 42..168 score: 5.8
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 15..166 score: 7.6
None	No IPR available	PANTHER	PTHR10209:SF107	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE-LIKE PROTEIN-RELATED	coord: 15..166 score: 7.6
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 13..168 score: 2.06

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI01G17050	Csa1G263960	Cucumber (Chinese Long) v2	cpicuB002
CSPI01G17050	CmaCh11G005090	Cucurbita maxima (Rimu)	cmacpiB097

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI01G17050	Cucurbita pepo (Zucchini)	cpecpiB351
CSPI01G17050	Cucurbita pepo (Zucchini)	cpecpiB640
CSPI01G17050	Melon (DHL92) v3.6.1	cpimedB051
CSPI01G17050	Cucumber (Gy14) v2	cgybcpiB002
CSPI01G17050	Silver-seed gourd	carcpiB0138
CSPI01G17050	Silver-seed gourd	carcpiB0608
CSPI01G17050	Cucumber (Chinese Long) v3	cpicucB000
CSPI01G17050	Watermelon (97103) v2	cpiwmbB068
CSPI01G17050	Wax gourd	cpiwgoB099
CSPI01G17050	Cucumber (Gy14) v1	cgycpiB518
CSPI01G17050	Cucurbita maxima (Rimu)	cmacpiB059
CSPI01G17050	Cucurbita moschata (Rifu)	cmocpiB051
CSPI01G17050	Cucurbita moschata (Rifu)	cmocpiB090
CSPI01G17050	Melon (DHL92) v3.5.1	cpimeB057
CSPI01G17050	Watermelon (Charleston Gray)	cpiwcgB071
CSPI01G17050	Watermelon (97103) v1	cpiwmB086