CSPI01G15420 (gene) Wild cucumber (PI 183967)

BLAST of CSPI01G15420 vs. Swiss-Prot
Match: TM10A_HUMAN (tRNA methyltransferase 10 homolog A OS=Homo sapiens GN=TRMT10A PE=1 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 5.2e-35
Identity = 112/331 (33.84%), Postives = 177/331 (53.47%), Query Frame = 1

Query: 2   EAVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKDI 61
           E  EES  P++   C          P+SK   KKL+KQ+++E ++     + ++QK+K+ 
Sbjct: 23  EDQEESQKPRLGEGCE---------PISKRQMKKLIKQKQWEEQR-----ELRKQKRKE- 82

Query: 62  ERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIVI 121
           +RKRK+ E                     R+ +M   S+  ++K  R          ++I
Sbjct: 83  KRKRKKLE---------------------RQCQMEPNSDGHDRKRVRRDVVHS-TLRLII 142

Query: 122 DLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDK-WI 181
           D  F HLM   +I  L +QI  CYA N R   P   +LT   G+++  +      DK W+
Sbjct: 143 DCSFDHLMVLKDIKKLHKQIQRCYAENRRALHPVQFYLTSHGGQLKKNMDE---NDKGWV 202

Query: 182 IEKECRSYIDALQE--QKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKA 241
             K+     +   E  +KE+L+YLT+DS  +L +LD  K Y++GGLVD N  KG+T K+A
Sbjct: 203 NWKDIHIKPEHYSELIKKEDLIYLTSDSPNILKELDESKAYVIGGLVDHNHHKGLTYKQA 262

Query: 242 QEQEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQR------- 301
            +  I  A+LP+G+++KM+S +VL VN V EI+L+YLETRDW+++FF ++PQR       
Sbjct: 263 SDYGINHAQLPLGNFVKMNSRKVLAVNHVFEIILEYLETRDWQEAFFTILPQRKGAVPTD 313

Query: 302 KRCGGDSNSDHPVDGEENGDGDNKNETKKTK 323
           K C   S+ +  V  EE G   + +E + ++
Sbjct: 323 KACESASHDNQSVRMEEGGSDSDSSEEEYSR 313

BLAST of CSPI01G15420 vs. Swiss-Prot
Match: TM10A_MOUSE (tRNA methyltransferase 10 homolog A OS=Mus musculus GN=Trmt10a PE=2 SV=2)

HSP 1 Score: 149.4 bits (376), Expect = 6.8e-35
Identity = 99/290 (34.14%), Postives = 153/290 (52.76%), Query Frame = 1

Query: 5   EESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKDIERK 64
           EE   P++D             P+SK   KKL+KQ+++E ++  +K + KE++K      
Sbjct: 26  EERQEPRVDAGA---------EPISKRQLKKLMKQKQWEEQREQRKEKRKEKRK------ 85

Query: 65  RKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIVIDLE 124
                                 R+ L + ++   S+  ++K  R   AR     +VID  
Sbjct: 86  ----------------------RKKLERRQLESNSDGNDRKRVRRDVARS-SLRLVIDCS 145

Query: 125 FSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWIIEKE 184
           F  LM   +I  L +QI  CYA N R   P   +LT   G+++  +        W+  K+
Sbjct: 146 FDDLMVLKDIKKLHKQIQRCYAENRRASHPVQFYLTSHGGQLKKNMDE--NDQGWVNWKD 205

Query: 185 CRSYIDALQE--QKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQEQE 244
                +   E  +KE+LVYLT+DS  VL DLD  K Y++GGLVD +  KG+T K+A    
Sbjct: 206 IHIKSEHYSELIKKEDLVYLTSDSPNVLKDLDESKAYVIGGLVDHSHHKGLTFKQATSYG 265

Query: 245 IQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRK 293
           I+ A+LP+  ++KM+S +VL VN V EI+L++LETRDW+++FF ++PQRK
Sbjct: 266 IEHAQLPLADFVKMNSRKVLAVNHVFEIILEFLETRDWQEAFFTILPQRK 275

BLAST of CSPI01G15420 vs. Swiss-Prot
Match: TRM10_SCHPO (tRNA (guanine(9)-N1)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=trm10 PE=1 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.6e-34
Identity = 109/302 (36.09%), Postives = 171/302 (56.62%), Query Frame = 1

Query: 25  EPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKDIERKRKEWEEKLASVTEEERAKLI 84
           +P LSK+A K+L +QQ ++A + +K+ + + +KK+               + +EER + I
Sbjct: 29  QPVLSKSALKRLKRQQEWDAGR-EKRAEMRREKKR---------------LRKEERKRKI 88

Query: 85  ESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIVIDLEFSHLMTPWEIHSLVQQIMYC 144
           E+         G+  + ++K+I RL         IV+D  F  LM   EI+SL QQ+  C
Sbjct: 89  EA---------GEVVKSQKKRI-RLGKVVPSSIRIVLDCAFDDLMNDKEINSLCQQVTRC 148

Query: 145 YAVNGRCPSPCHLWLTGCNGEMETQLQR-LPGF-DKWI-IEKECRSYIDALQEQKENLVY 204
           ++ N     P  L+ T   G ++T+    L G  + W       +SY++  + QKE LVY
Sbjct: 149 HSANRTALHPVELFATNFGGRLKTRQDFVLKGQQNNWKRYNPTTKSYLEEFESQKEKLVY 208

Query: 205 LTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQEQEIQTAKLPIGSYLKMSSSQV 264
           L+ADS+  + +LD  KIYI+G +VD+NR+K +   KA EQ I+TAKLPI  Y+K++  ++
Sbjct: 209 LSADSDNTITELDEDKIYIIGAIVDKNRYKNLCQNKASEQGIKTAKLPIDEYIKITDRKI 268

Query: 265 LTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDSNSDHPVD-GEENGDGDNKNETKK 323
           LTVNQV EIL  +LE RDW+ +F EV+P+RK  G    SD   D  E+     N+++++ 
Sbjct: 269 LTVNQVFEILSLWLEYRDWEKAFMEVIPKRK--GILLKSDESFDVSEDTRSQSNQSDSEL 302

BLAST of CSPI01G15420 vs. Swiss-Prot
Match: TM10A_XENTR (tRNA methyltransferase 10 homolog A OS=Xenopus tropicalis GN=trmt10a PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.7e-30
Identity = 111/346 (32.08%), Postives = 182/346 (52.60%), Query Frame = 1

Query: 1   MEAVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQ---AKEQK 60
           +EAV E     +DP       N  +   S++A      Q+   A +++ K Q   +K Q 
Sbjct: 3   LEAVTEPGKCSIDP-------NTKDLLASQHAGNNTPLQENSSAPRSECKAQDAMSKRQM 62

Query: 61  KKDIERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQ 120
           KK +  K+K+WE++     ++ + K    +R L ++   + + D   +       +    
Sbjct: 63  KKFL--KQKQWEDQRELRKQKRKEK--RQKRKLERQAQAEHNIDANSRKRFRHEVQPSAL 122

Query: 121 NIVIDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFD 180
            ++ID  F  LM   ++  L +QI  CYA N R   P  L+LT   G++++ +     +D
Sbjct: 123 RLIIDCSFDDLMALRDVKKLNKQIRRCYAENRRAVHPVQLYLTSHGGQLKSNMDE---YD 182

Query: 181 K-WIIEKECRSYIDALQE--QKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGIT 240
           K WI  K+     +  ++  +KE+LVYLT+DS  VL +LD  K YI+GGLVD N  KGIT
Sbjct: 183 KGWINWKDIHIKPEHYKDLIKKEDLVYLTSDSPEVLSELDETKAYIIGGLVDHNHHKGIT 242

Query: 241 MKKAQEQEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRC 300
            KKA E  I  A+LP+G+++KM++ +VL VN V EI+L +LE ++WK++FF V+PQRK  
Sbjct: 243 YKKALELGISHAQLPLGNFVKMNTRKVLAVNHVFEIILAFLEKKEWKEAFFSVLPQRKGA 302

Query: 301 --------GGDSNSDHPVDGEENGDGDNKNET----KKTKCDEVSS 329
                     +  +    DGE++    + +E+       +C+E +S
Sbjct: 303 IPLTETGEQPECRASEQEDGEDSDSDSSIDESATIQPSVRCEEQNS 334

BLAST of CSPI01G15420 vs. Swiss-Prot
Match: TM10A_BOVIN (tRNA methyltransferase 10 homolog A OS=Bos taurus GN=TRMT10A PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 6.6e-30
Identity = 111/344 (32.27%), Postives = 174/344 (50.58%), Query Frame = 1

Query: 15  PCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKDIERKRKEWEEKLAS 74
           P  S  CN       KN  +   + Q+  + +  + +  ++ KK     K+K+WEE+   
Sbjct: 7   PAFSETCNVER---QKNLSEDGEQNQKPGSSERFQPISKRQMKKL---MKQKQWEEQR-- 66

Query: 75  VTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQ----------NIVIDLE 134
                     E R+  RKE+  ++  +R+ + E  S   D  +           ++ID  
Sbjct: 67  ----------ELRKQKRKEKRKRKQLERQCQPESNSDGSDRKRIRRDVVHSPLRLIIDCS 126

Query: 135 FSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDK-WIIEK 194
           F  LM   +I  L +QI  CYA N R   P   +LT   G+++  +      DK W+  K
Sbjct: 127 FDSLMVLKDIKKLHKQIQRCYAENRRALHPVQFYLTSHGGQLKKNMDEN---DKGWVNWK 186

Query: 195 ECRSYIDALQE--QKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQEQ 254
           +     +   E  QKE+L+YLT+DS  +L +LD  K Y++GGLVD N  KG+T K+A + 
Sbjct: 187 DIHIKPEHYSEFIQKEDLIYLTSDSPNILKELDESKAYVIGGLVDHNHHKGLTYKQASDH 246

Query: 255 EIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRK--------- 314
            I  A+LP+G+++KM+S +VL VN V EI+L+YLETRDW+++FF ++PQRK         
Sbjct: 247 GIDHAQLPLGNFVKMNSRKVLAVNHVFEIILEYLETRDWQEAFFTILPQRKGAVPTDQAC 306

Query: 315 -RCGGDSNSDHPVDGEENGDG---DNKNETKKTKCDEVSSSDNN 333
             C  D      V+G  N D    +N++E   T  +E    +N+
Sbjct: 307 ESCSHDKKFAR-VEGGLNSDSSEEENRHELDSTHEEEKQDKENS 328

BLAST of CSPI01G15420 vs. TrEMBL
Match: A0A0A0LYJ3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G173210 PE=4 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 4.0e-183
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 1

Query: 1   MEAVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           MEAVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD
Sbjct: 1   MEAVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120
           IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV
Sbjct: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120

Query: 121 IDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240

Query: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDSNS 300
           QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDSNS
Sbjct: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDSNS 300

Query: 301 DHPVDGEENGDGDNKNETKKTKCDEVSSSDNNL 334
           DHPVDGEENGDGDNKNETKKTKCDEVSSSDNNL
Sbjct: 301 DHPVDGEENGDGDNKNETKKTKCDEVSSSDNNL 333

BLAST of CSPI01G15420 vs. TrEMBL
Match: B9T580_RICCO (RNA (Guanine-9-) methyltransferase domain containing, putative OS=Ricinus communis GN=RCOM_1065780 PE=4 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 4.3e-121
Identity = 239/331 (72.21%), Postives = 272/331 (82.18%), Query Frame = 1

Query: 4   VEESHNPKMDPPCSSSACNPP-----EPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKK 63
           VEE    ++D     +  NPP     +PPLSKNA+KKLLKQQRYEAKKA+KK   KE KK
Sbjct: 2   VEEDDATQIDQSALENHPNPPPQDQQQPPLSKNAQKKLLKQQRYEAKKAEKKALEKEHKK 61

Query: 64  KDIERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQN 123
           K+ ERKRKEW+E LA+V+EEER KLIESR+ LRKERM KRSE+RE KI+RL+ A+  GQ 
Sbjct: 62  KEAERKRKEWQETLATVSEEERTKLIESRQQLRKERMEKRSEERENKIQRLTEAKSNGQK 121

Query: 124 IVIDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDK 183
           IV+DLEFSHLMT  EIHSLVQQIMYCYAVNGRC  PCHLWLTGCNGEME QL RLPGFDK
Sbjct: 122 IVVDLEFSHLMTSSEIHSLVQQIMYCYAVNGRCALPCHLWLTGCNGEMENQLGRLPGFDK 181

Query: 184 WIIEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKA 243
           W+IEKE RSY++ALQ+ KE+LVYLTAD+ETVLD+LDLKK+YIVGGLVDRNRWKG+TMKKA
Sbjct: 182 WMIEKESRSYMEALQDHKESLVYLTADAETVLDELDLKKVYIVGGLVDRNRWKGLTMKKA 241

Query: 244 QEQEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDS 303
           QEQ IQTAKLPIGSY+KMSSSQVLTVNQVIEILLK+LETRDWK SFF+V+PQRKR    S
Sbjct: 242 QEQGIQTAKLPIGSYMKMSSSQVLTVNQVIEILLKFLETRDWKASFFQVIPQRKRYDTGS 301

Query: 304 NSDHPVDGEENGDGDNKNETKKTKCDEVSSS 330
             D  +DGEE  +  ++   KK KC EV  S
Sbjct: 302 TGDQEIDGEECEENKDEPYEKKKKCLEVPCS 332

BLAST of CSPI01G15420 vs. TrEMBL
Match: A5AJA8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_030149 PE=4 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 2.8e-120
Identity = 241/332 (72.59%), Postives = 278/332 (83.73%), Query Frame = 1

Query: 1   MEAVEESHNPKMDPPCSSSACNPPEPPL--SKNARKKLLKQQRYEAKKADKKLQAKEQKK 60
           MEAVE   N +  P        PP PPL  SKNA+KKLLKQQR EAKKA+KK   KEQKK
Sbjct: 1   MEAVEAERNDQNTPA-------PPPPPLTLSKNAQKKLLKQQRMEAKKAEKKAAMKEQKK 60

Query: 61  KDIERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQN 120
           K+ ERKRKEWEE LASV EEER+KLIESR+ LRKERM KRSE+REKK++RL+ A+++GQN
Sbjct: 61  KEAERKRKEWEEMLASVDEEERSKLIESRKGLRKERMEKRSEEREKKVQRLTNAKEHGQN 120

Query: 121 IVIDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDK 180
           IV+DL+FS LMTP EI+SLVQQIMYCYAVN RC SPCHLWLTGCNG+M +QLQRLPGFDK
Sbjct: 121 IVVDLDFSDLMTPNEINSLVQQIMYCYAVNRRCASPCHLWLTGCNGKMGSQLQRLPGFDK 180

Query: 181 WIIEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKA 240
           WIIEKE +SYI+ALQ++K NLVYLTADSET+LD+LD KKIYIVGGLVDRNRWKGITMKKA
Sbjct: 181 WIIEKENQSYIEALQDEKHNLVYLTADSETILDELDPKKIYIVGGLVDRNRWKGITMKKA 240

Query: 241 QEQEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDS 300
           QEQ IQTAKLPIG+YLKMSSSQVLTVNQVIEILLK+LET+DWK SFF+V+PQRKR G DS
Sbjct: 241 QEQGIQTAKLPIGNYLKMSSSQVLTVNQVIEILLKFLETKDWKASFFQVIPQRKRGGADS 300

Query: 301 N--SDHPVDGEENGDGDNKNETKKTKCDEVSS 329
                   +GEEN +G+++ E KK + +  S+
Sbjct: 301 EDCQGEVEEGEENEEGEDQLEMKKMRTESPSN 325

BLAST of CSPI01G15420 vs. TrEMBL
Match: W9QDU2_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_010619 PE=4 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 3.7e-120
Identity = 238/327 (72.78%), Postives = 271/327 (82.87%), Query Frame = 1

Query: 24  PEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKDIERKRKEWEEKLASVTEEERAKL 83
           P+ PLSKNA+KKLLKQQ+YEAKKA+KK QAKEQKKKD ERKRKEWEE LASV EEER+KL
Sbjct: 19  PQQPLSKNAQKKLLKQQKYEAKKAEKKAQAKEQKKKDAERKRKEWEETLASVPEEERSKL 78

Query: 84  IESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIVIDLEFSHLMTPWEIHSLVQQ--- 143
           IESR++LRKERM KRSE+REKKIERL+ A+  GQNI+IDL+FSHLMTP EI+SLVQQ   
Sbjct: 79  IESRKSLRKERMEKRSEEREKKIERLTNAKHSGQNIIIDLDFSHLMTPSEINSLVQQFSS 138

Query: 144 -------------------IMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWII 203
                              IMY YA+NGRC +P HLWLTGC GEM +QLQRLPGFDKWII
Sbjct: 139 LPFLSSFRRRSFVTDKSLQIMYSYAMNGRCANPGHLWLTGCAGEMGSQLQRLPGFDKWII 198

Query: 204 EKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQEQ 263
           EKE RSYI+ALQ+QKENLVYLTADSETVLD+LD KK+YI+GGLVDRNRWKGITMKKAQEQ
Sbjct: 199 EKENRSYIEALQDQKENLVYLTADSETVLDELDPKKLYIIGGLVDRNRWKGITMKKAQEQ 258

Query: 264 EIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDSNSD 323
            IQTAKLPIGSYLKMSSSQVLTVNQ IEILLK+LET+DWK SFF+V+PQRKRC  D+   
Sbjct: 259 GIQTAKLPIGSYLKMSSSQVLTVNQAIEILLKFLETKDWKTSFFQVIPQRKRCEADTQEH 318

Query: 324 HPVDGEENGDGDNKNETKKTKCDEVSS 329
           H  +GEEN + D++ +TK+ +C E  S
Sbjct: 319 HEAEGEENQESDDQTDTKR-QCIEAPS 344

BLAST of CSPI01G15420 vs. TrEMBL
Match: A0A067KUI9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_08957 PE=4 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 1.5e-118
Identity = 238/317 (75.08%), Postives = 266/317 (83.91%), Query Frame = 1

Query: 14  PPCSSSACNPPEP-PLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKDIERKRKEWEEKL 73
           PP        P+P PLSKNA+KKLLKQQ++EAKKA+KK   KEQKKK+ ERKRKEWEE L
Sbjct: 53  PPQDEQQPPSPQPQPLSKNAQKKLLKQQKFEAKKAEKKALMKEQKKKEAERKRKEWEETL 112

Query: 74  ASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIVIDLEFSHLMTPW 133
           A V+EEER KLIESRR LRKERM KRSE+RE KI+RL+ A+  GQ IVIDLEFSHLMT  
Sbjct: 113 AGVSEEEREKLIESRRELRKERMEKRSEERENKIQRLTEAKSNGQKIVIDLEFSHLMTSS 172

Query: 134 EIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWIIEKECRSYIDAL 193
           EIHSLVQQIMYCYAVNGRC +P  LWLTGC GEME+QLQRLPGFDKWIIEKE RSYI+AL
Sbjct: 173 EIHSLVQQIMYCYAVNGRCATPGLLWLTGCAGEMESQLQRLPGFDKWIIEKESRSYIEAL 232

Query: 194 QEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQEQEIQTAKLPIGS 253
           Q+QKENLVYLTAD+ET LD+LDLK+IYIVGGLVDRNRWKG+TMKKAQEQ IQTAKLPIG+
Sbjct: 233 QDQKENLVYLTADAETELDELDLKRIYIVGGLVDRNRWKGLTMKKAQEQGIQTAKLPIGN 292

Query: 254 YLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDSNSDH-PVDGEENGD 313
           +LKMSSSQVLTVNQVIEILLK+LET+DWKDSFF+V+PQRKRC  DS   H  VD EEN +
Sbjct: 293 HLKMSSSQVLTVNQVIEILLKFLETKDWKDSFFQVIPQRKRCDADSTEHHRGVDEEENEE 352

Query: 314 GDNKNETKKTKCDEVSS 329
            +   E KK    + SS
Sbjct: 353 KEESCENKKRCIQDPSS 369

BLAST of CSPI01G15420 vs. TAIR10
Match: AT5G47680.1 (AT5G47680.1 FUNCTIONS IN: molecular_function unknown)

HSP 1 Score: 389.4 bits (999), Expect = 2.2e-108
Identity = 215/323 (66.56%), Postives = 255/323 (78.95%), Query Frame = 1

Query: 3   AVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKDIE 62
           A     N   D P S  A     PPLSKNA+KK LKQQRYEAKKA+KK Q KE K+K+ E
Sbjct: 4   ATGNDDNGCFDKPESEPAPVNVSPPLSKNAQKKQLKQQRYEAKKAEKKAQEKEHKRKEGE 63

Query: 63  RKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIVID 122
           RK KEWEE LA+ TEEER KLIESR++LRKERM KRSE++EKKIERL+ A++ GQ IV+D
Sbjct: 64  RKLKEWEETLANATEEERLKLIESRKSLRKERMEKRSEEKEKKIERLNQAKEIGQKIVVD 123

Query: 123 LEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWIIE 182
           ++F+HLM+  EI SLVQQIMYCYAVNGR  SPCHLWLTG  G+M T+L +LPGF+KW IE
Sbjct: 124 VDFAHLMSESEISSLVQQIMYCYAVNGRSTSPCHLWLTGVQGKMSTELDKLPGFEKWFIE 183

Query: 183 KECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQEQE 242
           KE R YI+A+ +QK+NLVYLTADSETVLDDLD K IYI+GGLVDRNR+KGITM KAQEQ 
Sbjct: 184 KESRCYIEAMADQKDNLVYLTADSETVLDDLDPKHIYIIGGLVDRNRFKGITMTKAQEQG 243

Query: 243 IQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDSNSDH 302
           I+TAKLPIG Y+KMSSSQVLTVNQV+EIL+K+LETRDWK +FF V+PQRKR G D     
Sbjct: 244 IKTAKLPIGEYMKMSSSQVLTVNQVLEILVKFLETRDWKTAFFTVIPQRKRTGLD----- 303

Query: 303 PVDGEENGDGDNKNETKKTKCDE 326
           PVD  +      +++ K T+ +E
Sbjct: 304 PVDCSKLEHISEEHQEKDTEANE 321

BLAST of CSPI01G15420 vs. NCBI nr
Match: gi|449443504|ref|XP_004139517.1| (PREDICTED: tRNA (guanine(9)-N1)-methyltransferase [Cucumis sativus])

HSP 1 Score: 648.7 bits (1672), Expect = 5.7e-183
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 1

Query: 1   MEAVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           MEAVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD
Sbjct: 1   MEAVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120
           IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV
Sbjct: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120

Query: 121 IDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180

Query: 181 IEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE
Sbjct: 181 IEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240

Query: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDSNS 300
           QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDSNS
Sbjct: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDSNS 300

Query: 301 DHPVDGEENGDGDNKNETKKTKCDEVSSSDNNL 334
           DHPVDGEENGDGDNKNETKKTKCDEVSSSDNNL
Sbjct: 301 DHPVDGEENGDGDNKNETKKTKCDEVSSSDNNL 333

BLAST of CSPI01G15420 vs. NCBI nr
Match: gi|659128585|ref|XP_008464275.1| (PREDICTED: tRNA (guanine(9)-N1)-methyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 593.2 bits (1528), Expect = 2.8e-166
Identity = 307/327 (93.88%), Postives = 315/327 (96.33%), Query Frame = 1

Query: 1   MEAVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           ME VE  H+PK+DPPCS SACNPPEP LSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD
Sbjct: 1   MEDVEGGHSPKIDPPCSPSACNPPEPSLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120
           IERKRKEWEEKLA VTEEERAKLIESRRNLRKERMGKRSEDREKKIERL+TA+D GQNIV
Sbjct: 61  IERKRKEWEEKLACVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120

Query: 121 IDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSHLMTP EIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEM +QLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMGSQLQRLPGFDKWI 180

Query: 181 IEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKE RSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGIT+KKAQE
Sbjct: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITLKKAQE 240

Query: 241 QEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDSNS 300
           QEIQTA+LPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFF+VVPQRKRCG DSNS
Sbjct: 241 QEIQTARLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFQVVPQRKRCGADSNS 300

Query: 301 DHPVDGEENGDGDNKNETKKTKCDEVS 328
           DHPVDGEENGDGDNK ETKKTKCDEVS
Sbjct: 301 DHPVDGEENGDGDNKQETKKTKCDEVS 327

BLAST of CSPI01G15420 vs. NCBI nr
Match: gi|659128587|ref|XP_008464276.1| (PREDICTED: tRNA (guanine(9)-N1)-methyltransferase isoform X2 [Cucumis melo])

HSP 1 Score: 467.6 bits (1202), Expect = 1.8e-128
Identity = 243/260 (93.46%), Postives = 250/260 (96.15%), Query Frame = 1

Query: 1   MEAVEESHNPKMDPPCSSSACNPPEPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60
           ME VE  H+PK+DPPCS SACNPPEP LSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD
Sbjct: 1   MEDVEGGHSPKIDPPCSPSACNPPEPSLSKNARKKLLKQQRYEAKKADKKLQAKEQKKKD 60

Query: 61  IERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQNIV 120
           IERKRKEWEEKLA VTEEERAKLIESRRNLRKERMGKRSEDREKKIERL+TA+D GQNIV
Sbjct: 61  IERKRKEWEEKLACVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLNTAKDCGQNIV 120

Query: 121 IDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDKWI 180
           IDLEFSHLMTP EIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEM +QLQRLPGFDKWI
Sbjct: 121 IDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMGSQLQRLPGFDKWI 180

Query: 181 IEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKAQE 240
           IEKE RSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGIT+KKAQE
Sbjct: 181 IEKERRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITLKKAQE 240

Query: 241 QEIQTAKLPIGSYLKMSSSQ 261
           QEIQTA+LPIGSYLKMSSSQ
Sbjct: 241 QEIQTARLPIGSYLKMSSSQ 260

BLAST of CSPI01G15420 vs. NCBI nr
Match: gi|255585408|ref|XP_002533399.1| (PREDICTED: tRNA (guanine(9)-N1)-methyltransferase [Ricinus communis])

HSP 1 Score: 442.6 bits (1137), Expect = 6.2e-121
Identity = 239/331 (72.21%), Postives = 272/331 (82.18%), Query Frame = 1

Query: 4   VEESHNPKMDPPCSSSACNPP-----EPPLSKNARKKLLKQQRYEAKKADKKLQAKEQKK 63
           VEE    ++D     +  NPP     +PPLSKNA+KKLLKQQRYEAKKA+KK   KE KK
Sbjct: 2   VEEDDATQIDQSALENHPNPPPQDQQQPPLSKNAQKKLLKQQRYEAKKAEKKALEKEHKK 61

Query: 64  KDIERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQN 123
           K+ ERKRKEW+E LA+V+EEER KLIESR+ LRKERM KRSE+RE KI+RL+ A+  GQ 
Sbjct: 62  KEAERKRKEWQETLATVSEEERTKLIESRQQLRKERMEKRSEERENKIQRLTEAKSNGQK 121

Query: 124 IVIDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDK 183
           IV+DLEFSHLMT  EIHSLVQQIMYCYAVNGRC  PCHLWLTGCNGEME QL RLPGFDK
Sbjct: 122 IVVDLEFSHLMTSSEIHSLVQQIMYCYAVNGRCALPCHLWLTGCNGEMENQLGRLPGFDK 181

Query: 184 WIIEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKA 243
           W+IEKE RSY++ALQ+ KE+LVYLTAD+ETVLD+LDLKK+YIVGGLVDRNRWKG+TMKKA
Sbjct: 182 WMIEKESRSYMEALQDHKESLVYLTADAETVLDELDLKKVYIVGGLVDRNRWKGLTMKKA 241

Query: 244 QEQEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDS 303
           QEQ IQTAKLPIGSY+KMSSSQVLTVNQVIEILLK+LETRDWK SFF+V+PQRKR    S
Sbjct: 242 QEQGIQTAKLPIGSYMKMSSSQVLTVNQVIEILLKFLETRDWKASFFQVIPQRKRYDTGS 301

Query: 304 NSDHPVDGEENGDGDNKNETKKTKCDEVSSS 330
             D  +DGEE  +  ++   KK KC EV  S
Sbjct: 302 TGDQEIDGEECEENKDEPYEKKKKCLEVPCS 332

BLAST of CSPI01G15420 vs. NCBI nr
Match: gi|731437668|ref|XP_010647426.1| (PREDICTED: tRNA (guanine(9)-N1)-methyltransferase-like [Vitis vinifera])

HSP 1 Score: 439.9 bits (1130), Expect = 4.0e-120
Identity = 241/332 (72.59%), Postives = 278/332 (83.73%), Query Frame = 1

Query: 1   MEAVEESHNPKMDPPCSSSACNPPEPPL--SKNARKKLLKQQRYEAKKADKKLQAKEQKK 60
           MEAVE   N +  P        PP PPL  SKNA+KKLLKQQR EAKKA+KK   KEQKK
Sbjct: 1   MEAVEAERNDQNTPA-------PPPPPLTLSKNAQKKLLKQQRMEAKKAEKKAAMKEQKK 60

Query: 61  KDIERKRKEWEEKLASVTEEERAKLIESRRNLRKERMGKRSEDREKKIERLSTARDYGQN 120
           K+ ERKRKEWEE LASV EEER+KLIESR+ LRKERM KRSE+REKK++RL+ A+++GQN
Sbjct: 61  KEAERKRKEWEEMLASVDEEERSKLIESRKGLRKERMEKRSEEREKKVQRLTNAKEHGQN 120

Query: 121 IVIDLEFSHLMTPWEIHSLVQQIMYCYAVNGRCPSPCHLWLTGCNGEMETQLQRLPGFDK 180
           IV+DL+FS LMTP EI+SLVQQIMYCYAVN RC SPCHLWLTGCNG+M +QLQRLPGFDK
Sbjct: 121 IVVDLDFSDLMTPNEINSLVQQIMYCYAVNRRCASPCHLWLTGCNGKMGSQLQRLPGFDK 180

Query: 181 WIIEKECRSYIDALQEQKENLVYLTADSETVLDDLDLKKIYIVGGLVDRNRWKGITMKKA 240
           WIIEKE +SYI+ALQ++K NLVYLTADSET+LD+LD KKIYIVGGLVDRNRWKGITMKKA
Sbjct: 181 WIIEKENQSYIEALQDEKHNLVYLTADSETILDELDPKKIYIVGGLVDRNRWKGITMKKA 240

Query: 241 QEQEIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKDSFFEVVPQRKRCGGDS 300
           QEQ IQTAKLPIG+YLKMSSSQVLTVNQVIEILLK+LET+DWK SFF+V+PQRKR G DS
Sbjct: 241 QEQGIQTAKLPIGNYLKMSSSQVLTVNQVIEILLKFLETKDWKASFFQVIPQRKRGGADS 300

Query: 301 N--SDHPVDGEENGDGDNKNETKKTKCDEVSS 329
                   +GEEN +G+++ E KK + +  S+
Sbjct: 301 EDCQGEVEEGEENEEGEDQLEMKKMRTESPSN 325