CSPI01G13450 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI01G13450
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	rRNA intron-encoded homing endonuclease
Location	Chr1 : 8951330 .. 8951850 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGAGCACCGGTCGGCTCGTCCCTTCTGCCGGCGATGCGCTCCTGGCCTTAACTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGTATACATTAGCATGGGATAACGTCATAGGATTTCGATCCTATTTTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATTGGCGGATGTTGCTTTAAGGACTCCGCCAGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAA

mRNA sequence

ATGGTGAGCACCGGTCGGCTCGTCCCTTCTGCCGGCGATGCGCTCCTGGCCTTAACTGGCCGGGTCGTGCCTCCGGCGCTACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATTGGCGGATGTTGCTTTAAGGACTCCGCCAGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAA

Coding sequence (CDS)

ATGGTGAGCACCGGTCGGCTCGTCCCTTCTGCCGGCGATGCGCTCCTGGCCTTAACTGGCCGGGTCGTGCCTCCGGCGCTACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATTGGCGGATGTTGCTTTAAGGACTCCGCCAGCACCTTATGAGAAATCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAA

Homology

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BLAST of CSPI01G13450 vs. TrEMBL
Match: A0A0A0LDF7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G512980 PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 2.2e-53
Identity = 112/112 (100.00%), Postives = 112/112 (100.00%), Query Frame = 1


Query: 1   MVSTGRLVPSAGDALLALTGRVVPPALRTTAKAFAKDVFINQERKLGARRRSDTVLVSTI 60
           MVSTGRLVPSAGDALLALTGRVVPPALRTTAKAFAKDVFINQERKLGARRRSDTVLVSTI
Sbjct: 1   MVSTGRLVPSAGDALLALTGRVVPPALRTTAKAFAKDVFINQERKLGARRRSDTVLVSTI 60

Query: 61  NDADQGLADVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 113
           NDADQGLADVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 61  NDADQGLADVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 112

[Close]

BLAST of CSPI01G13450 vs. TrEMBL
Match: A0A072TTZ2_MEDTR (Uncharacterized protein OS=Medicago truncatula GN=MTR_0052s0110 PE=4 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 3.0e-42
Identity = 101/129 (78.29%), Postives = 102/129 (79.07%), Query Frame = 1


Query: 1   MVSTGRLVPSAGDALLALTGRVVPPAL-----------------RTTAKAFAKDVFINQE 60
           MV TG LVPSAGDALLAL GR   P +                 RTTAKAFAKDVFINQE
Sbjct: 212 MVCTGWLVPSAGDALLALIGRDSDPIVLAFGIGVMINRDSRGHSRTTAKAFAKDVFINQE 271

Query: 61  RKLGARRRSDTVLVSTINDADQGLADVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGR 113
           RKLGARRRSDTVLVSTINDADQG ADVA RTP APYEKSKSLGSGGSMVARLKLKGIDGR
Sbjct: 272 RKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSGGSMVARLKLKGIDGR 331

[Close]

BLAST of CSPI01G13450 vs. TrEMBL
Match: I1LXT1_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_13G013100 PE=4 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 9.1e-39
Identity = 94/122 (77.05%), Postives = 96/122 (78.69%), Query Frame = 1


Query: 2   VSTGRLVPSAGDALLALTGRVVPPALRTTAKAFAKDVFINQ-----------ERKLGARR 61
           V TGRLVPSAGDALL+LTGRVVPP       AF   V IN+           ERKLGARR
Sbjct: 123 VCTGRLVPSAGDALLSLTGRVVPPDSDPIVLAFGIGVMINRDSRGHSYFIVRERKLGARR 182

Query: 62  RSDTVLVSTINDADQGLADVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEP 113
           RSDTVLVSTINDADQG ADVA RTP APYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEP
Sbjct: 183 RSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEP 242

[Close]

BLAST of CSPI01G13450 vs. TrEMBL
Match: A0A089MX14_PENAM (rRNA intron-encoded homing endonuclease (Fragment) OS=Pennisetum americanum PE=2 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 2.2e-37
Identity = 83/85 (97.65%), Postives = 83/85 (97.65%), Query Frame = 1


Query: 28  RTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGLADVALRTPPAPYEKSKSLGS 87
           RTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQG ADVA RTPPAPYEKSKSLGS
Sbjct: 3   RTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAYRTPPAPYEKSKSLGS 62

Query: 88  GGSMVARLKLKGIDGRAPPGVEPAA 113
           GGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 63  GGSMVARLKLKGIDGRAPPGVEPAA 87

[Close]

BLAST of CSPI01G13450 vs. TrEMBL
Match: M1DMU8_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400041108 PE=4 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 3.8e-37
Identity = 83/89 (93.26%), Postives = 84/89 (94.38%), Query Frame = 1


Query: 24  PPALRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGLADVALRTPPAPYEKSK 83
           P  +RTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQG ADVA RTPPAPYEKSK
Sbjct: 51  PWLVRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPPAPYEKSK 110

Query: 84  SLGSGGSMVARLKLKGIDGRAPPGVEPAA 113
            LGSGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 111 FLGSGGSMVARLKLKGIDGRAPPGVEPAA 139

[Close]

BLAST of CSPI01G13450 vs. NCBI nr
Match: gi|700202973|gb|KGN58106.1| (hypothetical protein Csa_3G512980 [Cucumis sativus])

HSP 1 Score: 216.1 bits (549), Expect = 3.2e-53
Identity = 112/112 (100.00%), Postives = 112/112 (100.00%), Query Frame = 1


Query: 1   MVSTGRLVPSAGDALLALTGRVVPPALRTTAKAFAKDVFINQERKLGARRRSDTVLVSTI 60
           MVSTGRLVPSAGDALLALTGRVVPPALRTTAKAFAKDVFINQERKLGARRRSDTVLVSTI
Sbjct: 1   MVSTGRLVPSAGDALLALTGRVVPPALRTTAKAFAKDVFINQERKLGARRRSDTVLVSTI 60

Query: 61  NDADQGLADVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 113
           NDADQGLADVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 61  NDADQGLADVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEPAA 112

[Close]

BLAST of CSPI01G13450 vs. NCBI nr
Match: gi|922327215|ref|XP_013443000.1| (rRNA intron-encoded homing endonuclease, putative [Medicago truncatula])

HSP 1 Score: 179.1 bits (453), Expect = 4.3e-42
Identity = 101/129 (78.29%), Postives = 102/129 (79.07%), Query Frame = 1


Query: 1   MVSTGRLVPSAGDALLALTGRVVPPAL-----------------RTTAKAFAKDVFINQE 60
           MV TG LVPSAGDALLAL GR   P +                 RTTAKAFAKDVFINQE
Sbjct: 212 MVCTGWLVPSAGDALLALIGRDSDPIVLAFGIGVMINRDSRGHSRTTAKAFAKDVFINQE 271

Query: 61  RKLGARRRSDTVLVSTINDADQGLADVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGR 113
           RKLGARRRSDTVLVSTINDADQG ADVA RTP APYEKSKSLGSGGSMVARLKLKGIDGR
Sbjct: 272 RKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSGGSMVARLKLKGIDGR 331

[Close]

BLAST of CSPI01G13450 vs. NCBI nr
Match: gi|947068865|gb|KRH17756.1| (hypothetical protein GLYMA_13G013100 [Glycine max])

HSP 1 Score: 167.5 bits (423), Expect = 1.3e-38
Identity = 94/122 (77.05%), Postives = 96/122 (78.69%), Query Frame = 1


Query: 2   VSTGRLVPSAGDALLALTGRVVPPALRTTAKAFAKDVFINQ-----------ERKLGARR 61
           V TGRLVPSAGDALL+LTGRVVPP       AF   V IN+           ERKLGARR
Sbjct: 123 VCTGRLVPSAGDALLSLTGRVVPPDSDPIVLAFGIGVMINRDSRGHSYFIVRERKLGARR 182

Query: 62  RSDTVLVSTINDADQGLADVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEP 113
           RSDTVLVSTINDADQG ADVA RTP APYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEP
Sbjct: 183 RSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVEP 242

[Close]

BLAST of CSPI01G13450 vs. NCBI nr
Match: gi|686983426|gb|AIQ78384.1| (rRNA intron-encoded homing endonuclease, partial [Cenchrus americanus])

HSP 1 Score: 162.9 bits (411), Expect = 3.2e-37
Identity = 83/85 (97.65%), Postives = 83/85 (97.65%), Query Frame = 1


Query: 28  RTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGLADVALRTPPAPYEKSKSLGS 87
           RTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQG ADVA RTPPAPYEKSKSLGS
Sbjct: 3   RTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAYRTPPAPYEKSKSLGS 62

Query: 88  GGSMVARLKLKGIDGRAPPGVEPAA 113
           GGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 63  GGSMVARLKLKGIDGRAPPGVEPAA 87

[Close]

BLAST of CSPI01G13450 vs. NCBI nr
Match: gi|1012318265|gb|KYP31748.1| (hypothetical protein KK1_047782 [Cajanus cajan])

HSP 1 Score: 162.2 bits (409), Expect = 5.5e-37
Identity = 92/123 (74.80%), Postives = 94/123 (76.42%), Query Frame = 1


Query: 1   MVSTGRLVPSAGDALLALTGRVVPPALRTTAKAFAKDVFINQ-----------ERKLGAR 60
           +V T RLVPSAGDALL L GRVVPP       AF   V IN+           ERKLGAR
Sbjct: 72  LVCTDRLVPSAGDALLDLIGRVVPPDSDPIVLAFGIGVMINRDSRGHSYFIVRERKLGAR 131

Query: 61  RRSDTVLVSTINDADQGLADVALRTPPAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVE 113
           RRSDTVLVSTINDADQG ADVA RTP APYEKSKSLGSGGSMVARLKLKGIDGRAPPGVE
Sbjct: 132 RRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSGGSMVARLKLKGIDGRAPPGVE 191

The following BLAST results are available for this feature:

BLAST of CSPI01G13450 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of CSPI01G13450 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LDF7_CUCSA	2.2e-53	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G512980 PE=4 SV=1	[more]
A0A072TTZ2_MEDTR	3.0e-42	78.29	Uncharacterized protein OS=Medicago truncatula GN=MTR_0052s0110 PE=4 SV=1	[more]
I1LXT1_SOYBN	9.1e-39	77.05	Uncharacterized protein OS=Glycine max GN=GLYMA_13G013100 PE=4 SV=1	[more]
A0A089MX14_PENAM	2.2e-37	97.65	rRNA intron-encoded homing endonuclease (Fragment) OS=Pennisetum americanum PE=2...	[more]
M1DMU8_SOLTU	3.8e-37	93.26	Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400041108 PE=4 SV=1	[more]

BLAST of CSPI01G13450 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CSPI01G13450 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700202973\|gb\|KGN58106.1\|	3.2e-53	100.00	hypothetical protein Csa_3G512980 [Cucumis sativus]	[more]
gi\|922327215\|ref\|XP_013443000.1\|	4.3e-42	78.29	rRNA intron-encoded homing endonuclease, putative [Medicago truncatula]	[more]
gi\|947068865\|gb\|KRH17756.1\|	1.3e-38	77.05	hypothetical protein GLYMA_13G013100 [Glycine max]	[more]
gi\|686983426\|gb\|AIQ78384.1\|	3.2e-37	97.65	rRNA intron-encoded homing endonuclease, partial [Cenchrus americanus]	[more]
gi\|1012318265\|gb\|KYP31748.1\|	5.5e-37	74.80	hypothetical protein KK1_047782 [Cajanus cajan]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006506	GPI anchor biosynthetic process
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0016758	transferase activity, transferring hexosyl groups

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G13450.1	CSPI01G13450.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34410	FAMILY NOT NAMED	coord: 28..112 score: 5.4

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI01G13450	Cucumber (Gy14) v2	cgybcpiB001
CSPI01G13450	Silver-seed gourd	carcpiB0477
CSPI01G13450	Silver-seed gourd	carcpiB1105
CSPI01G13450	Cucumber (Chinese Long) v3	cpicucB001
CSPI01G13450	Watermelon (97103) v2	cpiwmbB034
CSPI01G13450	Wax gourd	cpiwgoB017
CSPI01G13450	Wax gourd	cpiwgoB049
CSPI01G13450	Wild cucumber (PI 183967)	cpicpiB017
CSPI01G13450	Cucurbita maxima (Rimu)	cmacpiB205
CSPI01G13450	Cucurbita maxima (Rimu)	cmacpiB434
CSPI01G13450	Cucurbita moschata (Rifu)	cmocpiB192
CSPI01G13450	Cucurbita moschata (Rifu)	cmocpiB425
CSPI01G13450	Cucumber (Chinese Long) v2	cpicuB000
CSPI01G13450	Cucumber (Chinese Long) v2	cpicuB026
CSPI01G13450	Melon (DHL92) v3.5.1	cpimeB011
CSPI01G13450	Watermelon (Charleston Gray)	cpiwcgB007
CSPI01G13450	Watermelon (Charleston Gray)	cpiwcgB040
CSPI01G13450	Watermelon (97103) v1	cpiwmB007
CSPI01G13450	Watermelon (97103) v1	cpiwmB012
CSPI01G13450	Cucurbita pepo (Zucchini)	cpecpiB518
CSPI01G13450	Cucurbita pepo (Zucchini)	cpecpiB530
CSPI01G13450	Bottle gourd (USVL1VR-Ls)	cpilsiB046
CSPI01G13450	Melon (DHL92) v3.6.1	cpimedB011