CSPI01G07610 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI01G07610
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Tumor-related protein
Location	Chr1 : 4824344 .. 4824832 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCATTTTGCATTACTCACTTGTCTTTTCATTGCCATTGTCATTGCCTCACCTGAGGTACGCTTCTGCAGAGCTGATGCGTCACCGGATCCCGTCCTCGACACCGATGGAAAGAAGCTCAGAATCAGCAACAAATACTACATCCTCCCAGCAATTCAAGGCAGTGTTGGTGGTGGAGGTTTCGCCATCGGGAATATCCGAAAAGAATACGATAGATGTGGTGTCAACGTCGTCCAGGAGTGTTACGAACAACCCGATGGTTTTTCCACTACATTTTTACCAATAAACCTTAAAAAGGGTGTGGTTTGAGTCTCCACCGATTTGAACATCGAGTTTGATGCGACTACAAGGTGTGTGAAATCGACGGTGTGGAGATTATGAACATTTGATCGATATTTGAGGTAATATTTCGTAACGATTGGCGGAACAAAGAGAATCCGGGGCAAGAGACGACGGAAAACTGGTTCAAAATAGAGCAATATGGGAAGG

mRNA sequence

ATGCATTTTGCATTACTCACTTGTCTTTTCATTGCCATTGTCATTGCCTCACCTGAGGTACGCTTCTGCAGAGCTGATGCGTCACCGGATCCCGTCCTCGACACCGATGGAAAGAAGCTCAGAATCAGCAACAAATACTACATCCTCCCAGCAATTCAAGGCAGTGTTGGTGGTGGAGGTTTCGCCATCGGGAATATCCGAAAAGAATACGATAGATGTGGTGTCAACGTCGTCCAGGAGTGTTACGAACAACCCGATGGTTTTTCCACTACATTTTTACCAATAAACCTTAAAAAGGGTGTGGTTTGA

Coding sequence (CDS)

ATGCATTTTGCATTACTCACTTGTCTTTTCATTGCCATTGTCATTGCCTCACCTGAGGTACGCTTCTGCAGAGCTGATGCGTCACCGGATCCCGTCCTCGACACCGATGGAAAGAAGCTCAGAATCAGCAACAAATACTACATCCTCCCAGCAATTCAAGGCAGTGTTGGTGGTGGAGGTTTCGCCATCGGGAATATCCGAAAAGAATACGATAGATGTGGTGTCAACGTCGTCCAGGAGTGTTACGAACAACCCGATGGTTTTTCCACTACATTTTTACCAATAAACCTTAAAAAGGGTGTGGTTTGA

Homology

[Close]

BLAST of CSPI01G07610 vs. Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 53.5 bits (127), Expect = 1.6e-06
Identity = 32/91 (35.16%), Postives = 43/91 (47.25%), Query Frame = 1


Query: 12  AIVIASPEVRFCRADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGGFAIGNIRKEYD 71
           A++ A+       AD++P+PVLD DG+KLR    YYI+P ++   GGG            
Sbjct: 16  ALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDH-GGGLTVSATTPNGTF 75

Query: 72  RCGVNVVQECYEQPDGFSTTFLPINLKKGVV 103
            C   VVQ   E        F P N K+ VV
Sbjct: 76  VCPPRVVQTRKEVDHDRPLAFFPENPKEDVV 105

[Close]

BLAST of CSPI01G07610 vs. Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 3.5e-06
Identity = 33/94 (35.11%), Postives = 47/94 (50.00%), Query Frame = 1


Query: 9   LFIAIVIASPEVRFCRADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGGFAIGNIRK 68
           + +     S    F  A+A+  PVLDTDG +L+   +YY+L +I G+ GGGG A+G  R 
Sbjct: 8   VLLLFAFTSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGA-GGGGLALG--RA 67

Query: 69  EYDRCGVNVVQECYEQPDGFSTTFLPINLKKGVV 103
               C   VVQ   +  +G    F   + K  VV
Sbjct: 68  TGQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVV 98

[Close]

BLAST of CSPI01G07610 vs. TrEMBL
Match: A0A0A0LR02_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043210 PE=4 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 7.5e-32
Identity = 70/73 (95.89%), Postives = 70/73 (95.89%), Query Frame = 1


Query: 1  MHFALLTCLFIAIVIASPEVRFCRADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGG 60
          MHFALLTCLFIAIVIAS EVRFC ADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGG 
Sbjct: 1  MHFALLTCLFIAIVIASSEVRFCGADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGS 60

Query: 61 FAIGNIRKEYDRC 74
          FAIGNIRKEYDRC
Sbjct: 61 FAIGNIRKEYDRC 73

[Close]

BLAST of CSPI01G07610 vs. TrEMBL
Match: A0A0A0LT81_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043220 PE=4 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.3e-20
Identity = 59/101 (58.42%), Postives = 69/101 (68.32%), Query Frame = 1


Query: 2   HFALLTCLFIAIVIASPEVRFCRADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGGF 61
           +FALL  LFI  VIAS EVRFCRADASPD VLDTDGKKLR  ++YYIL     S   GG 
Sbjct: 3   NFALLCFLFI--VIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY--SRNSGGL 62

Query: 62  AIGNIRKEYDRCGVNVVQECYEQPDGFSTTFLPINLKKGVV 103
           +IG I   Y++C +N++ E Y+   G   TF PIN KKGVV
Sbjct: 63  SIGGI-YGYEKCPINILPESYDYLHGLPATFSPINPKKGVV 98

[Close]

BLAST of CSPI01G07610 vs. TrEMBL
Match: A0A0A0LTW2_CUCSA (Tumor-related protein OS=Cucumis sativus GN=Csa_1G043200 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 8.0e-18
Identity = 51/94 (54.26%), Postives = 65/94 (69.15%), Query Frame = 1


Query: 10  FIAIVIASPE-VRFCRADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGGFAIGNIRK 69
           F+ I++AS + +RF  ADASP+ VLD DGKKLR    YYILP  +G   GGG  +GN++ 
Sbjct: 10  FLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGR--GGGLTLGNLQS 69

Query: 70  EYDRCGVNVVQECYEQPDGFSTTFLPINLKKGVV 103
           E  +C +NVVQE  E  +GF TTF P+N KKGVV
Sbjct: 70  E--KCPLNVVQEQLEVMNGFPTTFHPVNPKKGVV 99

[Close]

BLAST of CSPI01G07610 vs. TrEMBL
Match: A0A061EZK2_THECC (Kunitz family trypsin and protease inhibitor protein OS=Theobroma cacao GN=TCM_025233 PE=4 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.0e-12
Identity = 40/78 (51.28%), Postives = 49/78 (62.82%), Query Frame = 1


Query: 25  ADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGGFAIGNIRKEYDRCGVNVVQECYEQ 84
           A+A+PDPVLD  GKKLR    YYILP  +G   GGG  + +   E   C ++VVQE  E 
Sbjct: 15  ANAAPDPVLDISGKKLRTGTDYYILPVFRGR--GGGLTLASTGNE--SCPLDVVQEQLEV 74

Query: 85  PDGFSTTFLPINLKKGVV 103
            DG   TF P+N+KKGVV
Sbjct: 75  SDGLPVTFSPVNIKKGVV 88

[Close]

BLAST of CSPI01G07610 vs. TrEMBL
Match: V4TXV0_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10023572mg PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 1.7e-12
Identity = 40/79 (50.63%), Postives = 50/79 (63.29%), Query Frame = 1


Query: 24  RADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGGFAIGNIRKEYDRCGVNVVQECYE 83
           R +ASPDPVLD  GK+LR  +KYYILP  +G   GGG  +   R     C ++VVQE + 
Sbjct: 24  RGNASPDPVLDIAGKQLRAGSKYYILPVTKGQ--GGGLTLAG-RSNNKTCPLDVVQEQHS 83

Query: 84  QPDGFSTTFLPINLKKGVV 103
             +GF  TF P+N KKGVV
Sbjct: 84  LRNGFPVTFSPVNPKKGVV 99

[Close]

BLAST of CSPI01G07610 vs. TAIR10
Match: AT1G17860.1 (AT1G17860.1 Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 53.5 bits (127), Expect = 8.8e-08
Identity = 32/101 (31.68%), Postives = 50/101 (49.50%), Query Frame = 1


Query: 1   MHFALLTCLFIAIVIASPEVRFCRADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGG 60
           M   L   L +A+ I+    R    +A+ +PV D +GK L     YYILP I+G   GGG
Sbjct: 1   MSSLLYIFLLLAVFISH---RGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGR--GGG 60

Query: 61  FAIGNIRKEYDRCGVNVVQECYEQPDGFSTTFLPINLKKGV 102
             + N++ E   C  +V+Q+ +E   G    F P +  + +
Sbjct: 61  LTMSNLKTE--TCPTSVIQDQFEVSQGLPVKFSPYDKSRTI 94

[Close]

BLAST of CSPI01G07610 vs. NCBI nr
Match: gi|659066985|ref|XP_008437071.1| (PREDICTED: miraculin-like [Cucumis melo])

HSP 1 Score: 168.7 bits (426), Expect = 5.3e-39
Identity = 84/100 (84.00%), Postives = 89/100 (89.00%), Query Frame = 1


Query: 3   FALLTCLFIAIVIASPEVRFCRADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGGFA 62
           FALL+ LFIAIVIAS E+RFCRADASPD VLDTDGKKLR+SNKYYILPA +GS GGGG A
Sbjct: 4   FALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGS-GGGGLA 63

Query: 63  IGNIRKEYDRCGVNVVQECYEQPDGFSTTFLPINLKKGVV 103
           IGNIRKEYDRCG+NVVQE YEQ DG  TTFLPIN KKGVV
Sbjct: 64  IGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVV 102

[Close]

BLAST of CSPI01G07610 vs. NCBI nr
Match: gi|700209122|gb|KGN64218.1| (hypothetical protein Csa_1G043210 [Cucumis sativus])

HSP 1 Score: 144.4 bits (363), Expect = 1.1e-31
Identity = 70/73 (95.89%), Postives = 70/73 (95.89%), Query Frame = 1


Query: 1  MHFALLTCLFIAIVIASPEVRFCRADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGG 60
          MHFALLTCLFIAIVIAS EVRFC ADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGG 
Sbjct: 1  MHFALLTCLFIAIVIASSEVRFCGADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGS 60

Query: 61 FAIGNIRKEYDRC 74
          FAIGNIRKEYDRC
Sbjct: 61 FAIGNIRKEYDRC 73

[Close]

BLAST of CSPI01G07610 vs. NCBI nr
Match: gi|659066987|ref|XP_008437082.1| (PREDICTED: miraculin [Cucumis melo])

HSP 1 Score: 109.4 bits (272), Expect = 3.8e-21
Identity = 59/101 (58.42%), Postives = 70/101 (69.31%), Query Frame = 1


Query: 2   HFALLTCLFIAIVIASPEVRFCRADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGGF 61
           +FALL  LFI  VIAS EVRFCRADASPD VLDTDGKKLR  ++YYIL     S   GG 
Sbjct: 3   NFALLCFLFI--VIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY--SRNSGGL 62

Query: 62  AIGNIRKEYDRCGVNVVQECYEQPDGFSTTFLPINLKKGVV 103
           +IG I   Y++C +N++ E Y+  DG   TF P+N KKGVV
Sbjct: 63  SIGGI-YGYEKCPINILPESYDYLDGLPATFSPVNPKKGVV 98

[Close]

BLAST of CSPI01G07610 vs. NCBI nr
Match: gi|449439731|ref|XP_004137639.1| (PREDICTED: miraculin [Cucumis sativus])

HSP 1 Score: 107.1 bits (266), Expect = 1.9e-20
Identity = 59/101 (58.42%), Postives = 69/101 (68.32%), Query Frame = 1


Query: 2   HFALLTCLFIAIVIASPEVRFCRADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGGF 61
           +FALL  LFI  VIAS EVRFCRADASPD VLDTDGKKLR  ++YYIL     S   GG 
Sbjct: 3   NFALLCFLFI--VIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY--SRNSGGL 62

Query: 62  AIGNIRKEYDRCGVNVVQECYEQPDGFSTTFLPINLKKGVV 103
           +IG I   Y++C +N++ E Y+   G   TF PIN KKGVV
Sbjct: 63  SIGGI-YGYEKCPINILPESYDYLHGLPATFSPINPKKGVV 98

[Close]

BLAST of CSPI01G07610 vs. NCBI nr
Match: gi|659066983|ref|XP_008437058.1| (PREDICTED: miraculin-like [Cucumis melo])

HSP 1 Score: 104.8 bits (260), Expect = 9.4e-20
Identity = 54/93 (58.06%), Postives = 65/93 (69.89%), Query Frame = 1


Query: 10  FIAIVIASPEVRFCRADASPDPVLDTDGKKLRISNKYYILPAIQGSVGGGGFAIGNIRKE 69
           FI I++AS E+RF  ADASP+ VLD DGKKLR    YYILP  +G   GGG  +GN++ E
Sbjct: 10  FIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGR--GGGLTLGNLQSE 69

Query: 70  YDRCGVNVVQECYEQPDGFSTTFLPINLKKGVV 103
              C VNVVQE +E  +GF TTF P+N KKGVV
Sbjct: 70  I--CPVNVVQEQFELMNGFPTTFHPVNPKKGVV 98

The following BLAST results are available for this feature:

BLAST of CSPI01G07610 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MIRA_SYNDU	1.6e-06	35.16	Miraculin OS=Synsepalum dulcificum PE=1 SV=3	[more]
ASP_THECC	3.5e-06	35.11	21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1	[more]

BLAST of CSPI01G07610 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LR02_CUCSA	7.5e-32	95.89	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043210 PE=4 SV=1	[more]
A0A0A0LT81_CUCSA	1.3e-20	58.42	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G043220 PE=4 SV=1	[more]
A0A0A0LTW2_CUCSA	8.0e-18	54.26	Tumor-related protein OS=Cucumis sativus GN=Csa_1G043200 PE=4 SV=1	[more]
A0A061EZK2_THECC	1.0e-12	51.28	Kunitz family trypsin and protease inhibitor protein OS=Theobroma cacao GN=TCM_0...	[more]
V4TXV0_9ROSI	1.7e-12	50.63	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10023572mg PE=4 SV=1	[more]

BLAST of CSPI01G07610 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G17860.1	8.8e-08	31.68	Kunitz family trypsin and protease inhibitor protein	[more]

BLAST of CSPI01G07610 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659066985\|ref\|XP_008437071.1\|	5.3e-39	84.00	PREDICTED: miraculin-like [Cucumis melo]	[more]
gi\|700209122\|gb\|KGN64218.1\|	1.1e-31	95.89	hypothetical protein Csa_1G043210 [Cucumis sativus]	[more]
gi\|659066987\|ref\|XP_008437082.1\|	3.8e-21	58.42	PREDICTED: miraculin [Cucumis melo]	[more]
gi\|449439731\|ref\|XP_004137639.1\|	1.9e-20	58.42	PREDICTED: miraculin [Cucumis sativus]	[more]
gi\|659066983\|ref\|XP_008437058.1\|	9.4e-20	58.06	PREDICTED: miraculin-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002160	Prot_inh_Kunz-lg
IPR011065	Kunitz_inhibitor_STI-like_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0004866	endopeptidase inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004866	endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G07610.1	CSPI01G07610.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 32..99 score: 7.3
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 32..48 scor
IPR011065	Kunitz inhibitor ST1-like	unknown	SSF50386	STI-like	coord: 29..102 score: 1.48
None	No IPR available	GENE3D	G3DSA:2.80.10.50		coord: 28..102 score: 8.7
None	No IPR available	PANTHER	PTHR33107	FAMILY NOT NAMED	coord: 1..102 score: 2.9
None	No IPR available	PANTHER	PTHR33107:SF2	SUBFAMILY NOT NAMED	coord: 1..102 score: 2.9

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI01G07610	Silver-seed gourd	carcpiB0418
CSPI01G07610	Silver-seed gourd	carcpiB0531
CSPI01G07610	Cucumber (Chinese Long) v3	cpicucB001
CSPI01G07610	Watermelon (97103) v2	cpiwmbB010
CSPI01G07610	Wax gourd	cpiwgoB028
CSPI01G07610	Cucurbita maxima (Rimu)	cmacpiB394
CSPI01G07610	Cucurbita pepo (Zucchini)	cpecpiB025
CSPI01G07610	Cucumber (Gy14) v1	cgycpiB160
CSPI01G07610	Cucurbita maxima (Rimu)	cmacpiB218
CSPI01G07610	Cucurbita moschata (Rifu)	cmocpiB204
CSPI01G07610	Cucurbita moschata (Rifu)	cmocpiB383
CSPI01G07610	Cucumber (Chinese Long) v2	cpicuB000
CSPI01G07610	Melon (DHL92) v3.5.1	cpimeB024
CSPI01G07610	Watermelon (Charleston Gray)	cpiwcgB024
CSPI01G07610	Watermelon (97103) v1	cpiwmB042
CSPI01G07610	Cucurbita pepo (Zucchini)	cpecpiB505
CSPI01G07610	Bottle gourd (USVL1VR-Ls)	cpilsiB021
CSPI01G07610	Melon (DHL92) v3.6.1	cpimedB023
CSPI01G07610	Cucumber (Gy14) v2	cgybcpiB001