CSPI01G03450 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI01G03450
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionEF hand calcium-binding protein family
LocationChr1 : 2157719 .. 2158511 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCGTCGCCGTCGCCGTCTCCGTCGCTGGCTGTTTTGCAATTATTTTATTATTTTTTTCTTTCTCTGTTTGTTTATGAATCAATAGCATTGTGTTCGTCATTTGTGGAATCCCCGATAATGGCTGCACTAGCAGTTGAGCAGCTTAATCAATTACGAGACATTTTTGCACGATTTGACATGGATTCAGACGGAAGTCTTACAATTCTTGAATTAGCGGCATTGCTTCGATCTCTTGGTTTGAAGCCATCCGGTGATCAAATCCATGTTCTTCTTGCTAATATGGATTCGAATGGTAATGGATCTGTTGAGTTTGATGAATTGGTGACAGCGATTATGCCGGATTTCAATGAAGAGGTAATGGTAAATCAAACGCAACTTCTGGAGGTTTTCCGTTCGTTCGATCGAGATGGGAATGGATATATAACAGCGGCGGAACTGGCGGGATCTATGGCGAAAATGGGACAGCCATTGACGTACAGAGAATTAACGGAGATGATGAAGGAAGCAGATACGGATGGCGATGGAGTCATTAGCTTCAATGAATTTGCATCTGTAATGGCTAAATCTGCCGCTGATTTTCTAGGGCTTGCAATTTCTTGAGTGAATTAGGTCAGAATCATTTCTTCTTCTTTCTTCCTCCCCTTCTTCTTCTTCCTCCTTCCTCCTCTTCTTCTTCTTCTCTTCAATTTGAAAGTGTAATATAATTATCAAAAGTTTTCCCTCACTCTTATCTAATCAACAAGCACCAACGCCCCGCCCCGCCCCGCCATCATCTTAGAATCTTCCATTAACA

mRNA sequence

ATGGCTGCACTAGCAGTTGAGCAGCTTAATCAATTACGAGACATTTTTGCACGATTTGACATGGATTCAGACGGAAGTCTTACAATTCTTGAATTAGCGGCATTGCTTCGATCTCTTGGTTTGAAGCCATCCGGTGATCAAATCCATGTTCTTCTTGCTAATATGGATTCGAATGGTAATGGATCTGTTGAGTTTGATGAATTGGTGACAGCGATTATGCCGGATTTCAATGAAGAGGTAATGGTAAATCAAACGCAACTTCTGGAGGTTTTCCGTTCGTTCGATCGAGATGGGAATGGATATATAACAGCGGCGGAACTGGCGGGATCTATGGCGAAAATGGGACAGCCATTGACGTACAGAGAATTAACGGAGATGATGAAGGAAGCAGATACGGATGGCGATGGAGTCATTAGCTTCAATGAATTTGCATCTGTAATGGCTAAATCTGCCGCTGATTTTCTAGGGCTTGCAATTTCTTGA

Coding sequence (CDS)

ATGGCTGCACTAGCAGTTGAGCAGCTTAATCAATTACGAGACATTTTTGCACGATTTGACATGGATTCAGACGGAAGTCTTACAATTCTTGAATTAGCGGCATTGCTTCGATCTCTTGGTTTGAAGCCATCCGGTGATCAAATCCATGTTCTTCTTGCTAATATGGATTCGAATGGTAATGGATCTGTTGAGTTTGATGAATTGGTGACAGCGATTATGCCGGATTTCAATGAAGAGGTAATGGTAAATCAAACGCAACTTCTGGAGGTTTTCCGTTCGTTCGATCGAGATGGGAATGGATATATAACAGCGGCGGAACTGGCGGGATCTATGGCGAAAATGGGACAGCCATTGACGTACAGAGAATTAACGGAGATGATGAAGGAAGCAGATACGGATGGCGATGGAGTCATTAGCTTCAATGAATTTGCATCTGTAATGGCTAAATCTGCCGCTGATTTTCTAGGGCTTGCAATTTCTTGA
Homology
[Close]
BLAST of CSPI01G03450 vs. Swiss-Prot
Match: CML15_ARATH (Probable calcium-binding protein CML15 OS=Arabidopsis thaliana GN=CML15 PE=2 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 8.0e-66
Identity = 123/154 (79.87%), Postives = 142/154 (92.21%), Query Frame = 1 

Query: 7   EQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFD 66
           +Q+ QL+DIF RFDMD+DGSLTILELAALLRSLGLKPSGDQIHVLLA+MDSNGNG VEFD
Sbjct: 3   DQIRQLKDIFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEFD 62

Query: 67  ELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEM 126
           ELV  I+PD NEEV++N  QLLE+F+SFDRDGNG+I+AAELAG+MAKMGQPLTY+ELTEM
Sbjct: 63  ELVGTILPDLNEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEM 122

Query: 127 MKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 161
           +KEADT+GDGVISF EFAS+MAKSA D+ GL I+
Sbjct: 123 IKEADTNGDGVISFGEFASIMAKSAVDYFGLKIN 156

[Close]
BLAST of CSPI01G03450 vs. Swiss-Prot
Match: CML16_ARATH (Probable calcium-binding protein CML16 OS=Arabidopsis thaliana GN=CML16 PE=2 SV=2)

HSP 1 Score: 245.0 bits (624), Expect = 5.7e-64
Identity = 123/154 (79.87%), Postives = 137/154 (88.96%), Query Frame = 1 

Query: 7   EQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFD 66
           +Q+ QL+DIFARFDMD DGSLT LELAALLRSLG+KP GDQI +LL  +D NGNGSVEFD
Sbjct: 8   DQIKQLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNGNGSVEFD 67

Query: 67  ELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEM 126
           ELV AI+PD NEEV++NQ QL+EVFRSFDRDGNG ITAAELAGSMAKMG PLTYRELTEM
Sbjct: 68  ELVVAILPDINEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTEM 127

Query: 127 MKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 161
           M EAD++GDGVISFNEF+ +MAKSAADFLGL  S
Sbjct: 128 MTEADSNGDGVISFNEFSHIMAKSAADFLGLTAS 161

[Close]
BLAST of CSPI01G03450 vs. Swiss-Prot
Match: CML14_ORYSJ (Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica GN=CML14 PE=2 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 3.4e-56
Identity = 110/152 (72.37%), Postives = 131/152 (86.18%), Query Frame = 1 

Query: 8   QLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFDE 67
           QL QLR++F RFDM+ DGSLT LELAALLRSLGL+P+GD++H LLA MD+NGNGSVEFDE
Sbjct: 22  QLKQLRELFRRFDMNGDGSLTQLELAALLRSLGLRPTGDEVHALLAGMDANGNGSVEFDE 81

Query: 68  LVTAIMPDFNEEV-MVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEM 127
           L  AI P    +  +V+Q QLLEVFR+FDRDGNG+I+AAELA SMA++GQPLT+ ELT M
Sbjct: 82  LAAAIAPVLTTQTHLVDQAQLLEVFRAFDRDGNGFISAAELARSMARLGQPLTFEELTRM 141

Query: 128 MKEADTDGDGVISFNEFASVMAKSAADFLGLA 159
           M++ADTDGDGVISF EFA+VMAKSA DFLG+A
Sbjct: 142 MRDADTDGDGVISFKEFAAVMAKSALDFLGVA 173

[Close]
BLAST of CSPI01G03450 vs. Swiss-Prot
Match: CML12_ORYSJ (Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica GN=CML12 PE=2 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 2.8e-50
Identity = 104/162 (64.20%), Postives = 129/162 (79.63%), Query Frame = 1 

Query: 2   AALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPS-GDQIHVLLANMDSNGN 61
           A L  EQL QL +IF RFD+D DGSLT LELAALLRSLGL+P+ GD+IH L+A +D++GN
Sbjct: 86  ARLRGEQLRQLHEIFLRFDLDGDGSLTKLELAALLRSLGLRPAAGDEIHALIAAIDADGN 145

Query: 62  GSVEFDELVTA----IMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQ 121
           G+VEFDEL ++    I+      V V+Q +L E FR+FDRDGNG+I+AAELA SMA+MG 
Sbjct: 146 GTVEFDELASSLADLILGPCRPSVAVDQAELAEAFRAFDRDGNGFISAAELARSMARMGH 205

Query: 122 PLTYRELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLA 159
           P+ Y ELT+MM+EADTDGDG+ISF EF ++MAKSA DFLGLA
Sbjct: 206 PICYAELTDMMREADTDGDGLISFEEFTAIMAKSALDFLGLA 247

[Close]
BLAST of CSPI01G03450 vs. Swiss-Prot
Match: CML18_ARATH (Probable calcium-binding protein CML18 OS=Arabidopsis thaliana GN=CML18 PE=1 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 2.4e-38
Identity = 87/152 (57.24%), Postives = 100/152 (65.79%), Query Frame = 1 

Query: 2   AALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNG 61
           A L  EQL +LR+IF  FD + DGSLT LEL +LLRSLGLKPS DQ+  L+   D N NG
Sbjct: 11  AKLGDEQLAELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNNG 70

Query: 62  SVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYR 121
            VEF E V  + PD   +      QL  +FR FDRDGNGYITAAELA SMAK+G  LT  
Sbjct: 71  LVEFSEFVALVEPDL-VKCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALTAE 130

Query: 122 ELTEMMKEADTDGDGVISFNEFASVMAKSAAD 154
           ELT M+KEAD DGDG I F EF   +  +A D
Sbjct: 131 ELTGMIKEADRDGDGCIDFQEFVQAITSAAFD 161

[Close]
BLAST of CSPI01G03450 vs. TrEMBL
Match: A0A0A0LUS7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G015780 PE=4 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 1.0e-80
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 1 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60
           MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN
Sbjct: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 161
           RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS
Sbjct: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 160

[Close]
BLAST of CSPI01G03450 vs. TrEMBL
Match: M5WF45_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa022918mg PE=4 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 3.0e-72
Identity = 142/160 (88.75%), Postives = 154/160 (96.25%), Query Frame = 1 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60
           M ALAV+QLNQLR+IFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN
Sbjct: 1   MPALAVDQLNQLREIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELV+AI+PD NEE++ NQ QLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GSVEFDELVSAILPDMNEEILGNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 161
           +ELTE++KEADTDGDGVISFNEFA++MAKSA+ FLGLA S
Sbjct: 121 KELTEIIKEADTDGDGVISFNEFATIMAKSASSFLGLAPS 160

[Close]
BLAST of CSPI01G03450 vs. TrEMBL
Match: A0A067FQ34_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g042615mg PE=4 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 2.4e-69
Identity = 133/160 (83.12%), Postives = 149/160 (93.12%), Query Frame = 1 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60
           M  L ++QLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMD+NGN
Sbjct: 1   MGGLHIDQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDANGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           G+VEFDELV AI+PD +E +++NQ QLLE+FR+FDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GAVEFDELVNAILPDMDETILINQEQLLEIFRAFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 161
           +ELTEM+KEADT+GDGVISFNEFA+VMAKS  DF GL +S
Sbjct: 121 KELTEMIKEADTNGDGVISFNEFAAVMAKSTLDFFGLGLS 160

[Close]
BLAST of CSPI01G03450 vs. TrEMBL
Match: V4VMQ6_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10023347mg PE=4 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 2.4e-69
Identity = 133/160 (83.12%), Postives = 149/160 (93.12%), Query Frame = 1 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60
           M  L ++QLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMD+NGN
Sbjct: 1   MGGLHIDQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDANGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           G+VEFDELV AI+PD +E +++NQ QLLE+FR+FDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GAVEFDELVNAILPDMDETILINQEQLLEIFRAFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 161
           +ELTEM+KEADT+GDGVISFNEFA+VMAKS  DF GL +S
Sbjct: 121 KELTEMIKEADTNGDGVISFNEFAAVMAKSTLDFFGLGLS 160

[Close]
BLAST of CSPI01G03450 vs. TrEMBL
Match: A0A061FXL0_THECC (EF hand calcium-binding protein family OS=Theobroma cacao GN=TCM_014474 PE=4 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 9.2e-69
Identity = 134/160 (83.75%), Postives = 148/160 (92.50%), Query Frame = 1 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60
           M +L V+Q+NQL+DIFARFDMD+DGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN
Sbjct: 1   MVSLRVDQINQLKDIFARFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           G+VEFDELV AI+PD NE+++VNQ QL EVF  FDRDGNGYITAAELAG MAKMGQ LTY
Sbjct: 61  GAVEFDELVNAILPDMNEQILVNQEQLREVFHLFDRDGNGYITAAELAGCMAKMGQSLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 161
           RELTEM+KEADTDGDGVISFNEF+SVMAKSA DFLG+ +S
Sbjct: 121 RELTEMIKEADTDGDGVISFNEFSSVMAKSALDFLGITLS 160

[Close]
BLAST of CSPI01G03450 vs. TAIR10
Match: AT1G18530.1 (AT1G18530.1 EF hand calcium-binding protein family)

HSP 1 Score: 251.1 bits (640), Expect = 4.5e-67
Identity = 123/154 (79.87%), Postives = 142/154 (92.21%), Query Frame = 1 

Query: 7   EQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFD 66
           +Q+ QL+DIF RFDMD+DGSLTILELAALLRSLGLKPSGDQIHVLLA+MDSNGNG VEFD
Sbjct: 3   DQIRQLKDIFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEFD 62

Query: 67  ELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEM 126
           ELV  I+PD NEEV++N  QLLE+F+SFDRDGNG+I+AAELAG+MAKMGQPLTY+ELTEM
Sbjct: 63  ELVGTILPDLNEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEM 122

Query: 127 MKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 161
           +KEADT+GDGVISF EFAS+MAKSA D+ GL I+
Sbjct: 123 IKEADTNGDGVISFGEFASIMAKSAVDYFGLKIN 156

[Close]
BLAST of CSPI01G03450 vs. TAIR10
Match: AT3G25600.1 (AT3G25600.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 245.0 bits (624), Expect = 3.2e-65
Identity = 123/154 (79.87%), Postives = 137/154 (88.96%), Query Frame = 1 

Query: 7   EQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFD 66
           +Q+ QL+DIFARFDMD DGSLT LELAALLRSLG+KP GDQI +LL  +D NGNGSVEFD
Sbjct: 8   DQIKQLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNGNGSVEFD 67

Query: 67  ELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRELTEM 126
           ELV AI+PD NEEV++NQ QL+EVFRSFDRDGNG ITAAELAGSMAKMG PLTYRELTEM
Sbjct: 68  ELVVAILPDINEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTEM 127

Query: 127 MKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 161
           M EAD++GDGVISFNEF+ +MAKSAADFLGL  S
Sbjct: 128 MTEADSNGDGVISFNEFSHIMAKSAADFLGLTAS 161

[Close]
BLAST of CSPI01G03450 vs. TAIR10
Match: AT3G03000.1 (AT3G03000.1 EF hand calcium-binding protein family)

HSP 1 Score: 159.8 bits (403), Expect = 1.4e-39
Identity = 87/152 (57.24%), Postives = 100/152 (65.79%), Query Frame = 1 

Query: 2   AALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNG 61
           A L  EQL +LR+IF  FD + DGSLT LEL +LLRSLGLKPS DQ+  L+   D N NG
Sbjct: 11  AKLGDEQLAELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNNG 70

Query: 62  SVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYR 121
            VEF E V  + PD   +      QL  +FR FDRDGNGYITAAELA SMAK+G  LT  
Sbjct: 71  LVEFSEFVALVEPDL-VKCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALTAE 130

Query: 122 ELTEMMKEADTDGDGVISFNEFASVMAKSAAD 154
           ELT M+KEAD DGDG I F EF   +  +A D
Sbjct: 131 ELTGMIKEADRDGDGCIDFQEFVQAITSAAFD 161

[Close]
BLAST of CSPI01G03450 vs. TAIR10
Match: AT1G32250.1 (AT1G32250.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 152.5 bits (384), Expect = 2.2e-37
Identity = 81/151 (53.64%), Postives = 101/151 (66.89%), Query Frame = 1 

Query: 7   EQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGSVEFD 66
           EQ+N+LR+IF  FD + DGSLT LEL +LLR+LG+KPS DQ   L+   D+  NG VEF 
Sbjct: 12  EQINELREIFRSFDRNKDGSLTQLELGSLLRALGVKPSPDQFETLIDKADTKSNGLVEFP 71

Query: 67  ELVTAIMPDF----NEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRE 126
           E V  + P+            + QLL +FR FD DGNG+ITAAELA SMAK+G  LT  E
Sbjct: 72  EFVALVSPELLSPAKRTTPYTEEQLLRLFRIFDTDGNGFITAAELAHSMAKLGHALTVAE 131

Query: 127 LTEMMKEADTDGDGVISFNEFASVMAKSAAD 154
           LT M+KEAD+DGDG I+F EFA  +  +A D
Sbjct: 132 LTGMIKEADSDGDGRINFQEFAKAINSAAFD 162

[Close]
BLAST of CSPI01G03450 vs. TAIR10
Match: AT4G14640.1 (AT4G14640.1 calmodulin 8)

HSP 1 Score: 119.0 bits (297), Expect = 2.7e-27
Identity = 56/145 (38.62%), Postives = 93/145 (64.14%), Query Frame = 1 

Query: 3   ALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGNGS 62
           AL  +Q+ + ++ F  FD D DG +T+ ELA ++RSL   P+  ++H ++  +DS+ NG+
Sbjct: 5   ALTKDQITEFKEAFCLFDKDGDGCITVEELATVIRSLDQNPTEQELHDIITEIDSDSNGT 64

Query: 63  VEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTYRE 122
           +EF E +  +     E     + +L E F+ FD+D NGYI+A+EL+  M  +G+ LT  E
Sbjct: 65  IEFAEFLNLMAKKLQESDA--EEELKEAFKVFDKDQNGYISASELSHVMINLGEKLTDEE 124

Query: 123 LTEMMKEADTDGDGVISFNEFASVM 148
           + +M+KEAD DGDG ++++EF  +M
Sbjct: 125 VEQMIKEADLDGDGQVNYDEFVKMM 147

[Close]
BLAST of CSPI01G03450 vs. NCBI nr
Match: gi|778656252|ref|XP_004138337.2| (PREDICTED: probable calcium-binding protein CML15 [Cucumis sativus])

HSP 1 Score: 307.4 bits (786), Expect = 1.5e-80
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 1 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60
           MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN
Sbjct: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 161
           RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS
Sbjct: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 160

[Close]
BLAST of CSPI01G03450 vs. NCBI nr
Match: gi|659106481|ref|XP_008453344.1| (PREDICTED: probable calcium-binding protein CML15 [Cucumis melo])

HSP 1 Score: 305.8 bits (782), Expect = 4.4e-80
Identity = 159/160 (99.38%), Postives = 160/160 (100.00%), Query Frame = 1 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60
           MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN
Sbjct: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELVTAIMPDFNEEVMVNQT+LLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GSVEFDELVTAIMPDFNEEVMVNQTRLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 161
           RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS
Sbjct: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 160

[Close]
BLAST of CSPI01G03450 vs. NCBI nr
Match: gi|694319324|ref|XP_009346595.1| (PREDICTED: probable calcium-binding protein CML15 [Pyrus x bretschneideri])

HSP 1 Score: 282.0 bits (720), Expect = 6.7e-73
Identity = 143/160 (89.38%), Postives = 154/160 (96.25%), Query Frame = 1 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60
           M AL V+QLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMD+NGN
Sbjct: 1   MPALRVDQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDANGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELV+AI+PD NEE++VNQ QLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GSVEFDELVSAILPDMNEEILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 161
           +ELTEM+KEADTDGDGVISFNEFA++MAKSA+ FLGLA S
Sbjct: 121 KELTEMIKEADTDGDGVISFNEFATIMAKSASSFLGLAPS 160

[Close]
BLAST of CSPI01G03450 vs. NCBI nr
Match: gi|1009132004|ref|XP_015883151.1| (PREDICTED: probable calcium-binding protein CML15 [Ziziphus jujuba])

HSP 1 Score: 282.0 bits (720), Expect = 6.7e-73
Identity = 142/160 (88.75%), Postives = 155/160 (96.88%), Query Frame = 1 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60
           + AL V+QLNQL+DIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN
Sbjct: 2   LGALHVDQLNQLKDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 61

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELV AI+PD +EE+++NQ QLLEVFRSFDRDGNGYI+AAELAGSMAKMGQPLTY
Sbjct: 62  GSVEFDELVNAILPDMSEEILINQEQLLEVFRSFDRDGNGYISAAELAGSMAKMGQPLTY 121

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 161
           +ELTEM+KEADTDGDGVISFNEFASVMAKSA+DFLGLA+S
Sbjct: 122 KELTEMIKEADTDGDGVISFNEFASVMAKSASDFLGLAMS 161

[Close]
BLAST of CSPI01G03450 vs. NCBI nr
Match: gi|657966069|ref|XP_008374715.1| (PREDICTED: probable calcium-binding protein CML15 [Malus domestica])

HSP 1 Score: 280.8 bits (717), Expect = 1.5e-72
Identity = 142/160 (88.75%), Postives = 154/160 (96.25%), Query Frame = 1 

Query: 1   MAALAVEQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDSNGN 60
           M AL V+QLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMD+NGN
Sbjct: 1   MPALRVDQLNQLRDIFARFDMDSDGSLTILELAALLRSLGLKPSGDQIHVLLANMDANGN 60

Query: 61  GSVEFDELVTAIMPDFNEEVMVNQTQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120
           GSVEFDELV+AI+PD NEE++VNQ QLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY
Sbjct: 61  GSVEFDELVSAILPDMNEEILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGQPLTY 120

Query: 121 RELTEMMKEADTDGDGVISFNEFASVMAKSAADFLGLAIS 161
           +ELTEM+KEADTDGDGVISFNEF+++MAKSA+ FLGLA S
Sbjct: 121 KELTEMIKEADTDGDGVISFNEFSTIMAKSASSFLGLAPS 160

The following BLAST results are available for this feature:
BLAST of CSPI01G03450 vs. Swiss-Prot
Match NameE-valueIdentityDescription
CML15_ARATH8.0e-6679.87Probable calcium-binding protein CML15 OS=Arabidopsis thaliana GN=CML15 PE=2 SV=... [more]
CML16_ARATH5.7e-6479.87Probable calcium-binding protein CML16 OS=Arabidopsis thaliana GN=CML16 PE=2 SV=... [more]
CML14_ORYSJ3.4e-5672.37Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica GN=CML14 ... [more]
CML12_ORYSJ2.8e-5064.20Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica GN=CML12 ... [more]
CML18_ARATH2.4e-3857.24Probable calcium-binding protein CML18 OS=Arabidopsis thaliana GN=CML18 PE=1 SV=... [more]
BLAST of CSPI01G03450 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LUS7_CUCSA1.0e-80100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G015780 PE=4 SV=1[more]
M5WF45_PRUPE3.0e-7288.75Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa022918mg PE=4 SV=1[more]
A0A067FQ34_CITSI2.4e-6983.13Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g042615mg PE=4 SV=1[more]
V4VMQ6_9ROSI2.4e-6983.13Uncharacterized protein OS=Citrus clementina GN=CICLE_v10023347mg PE=4 SV=1[more]
A0A061FXL0_THECC9.2e-6983.75EF hand calcium-binding protein family OS=Theobroma cacao GN=TCM_014474 PE=4 SV=... [more]
BLAST of CSPI01G03450 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G18530.14.5e-6779.87 EF hand calcium-binding protein family[more]
AT3G25600.13.2e-6579.87 Calcium-binding EF-hand family protein[more]
AT3G03000.11.4e-3957.24 EF hand calcium-binding protein family[more]
AT1G32250.12.2e-3753.64 Calcium-binding EF-hand family protein[more]
AT4G14640.12.7e-2738.62 calmodulin 8[more]
BLAST of CSPI01G03450 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778656252|ref|XP_004138337.2|1.5e-80100.00PREDICTED: probable calcium-binding protein CML15 [Cucumis sativus][more]
gi|659106481|ref|XP_008453344.1|4.4e-8099.38PREDICTED: probable calcium-binding protein CML15 [Cucumis melo][more]
gi|694319324|ref|XP_009346595.1|6.7e-7389.38PREDICTED: probable calcium-binding protein CML15 [Pyrus x bretschneideri][more]
gi|1009132004|ref|XP_015883151.1|6.7e-7388.75PREDICTED: probable calcium-binding protein CML15 [Ziziphus jujuba][more]
gi|657966069|ref|XP_008374715.1|1.5e-7288.75PREDICTED: probable calcium-binding protein CML15 [Malus domestica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010200 response to chitin
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006468 protein phosphorylation
biological_process GO:0009069 serine family amino acid metabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0032440 2-alkenal reductase [NAD(P)] activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G03450.1CSPI01G03450.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 85..147
score: 1.2E-14coord: 10..71
score: 5.6
IPR002048EF-hand domainSMARTSM00054efh_1coord: 86..114
score: 5.7E-4coord: 11..39
score: 0.046coord: 122..150
score: 8.4E-8coord: 47..75
score:
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 82..117
score: 14.039coord: 7..42
score: 13.481coord: 43..78
score: 9.13coord: 118..153
score: 14
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 5..149
score: 2.1
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 4..148
score: 2.84
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 20..32
score: -coord: 56..68
score: -coord: 95..107
score: -coord: 131..143
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 1..159
score: 2.4E
NoneNo IPR availablePANTHERPTHR10891:SF638CALCIUM-BINDING PROTEIN CML15-RELATEDcoord: 1..159
score: 2.4E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI01G03450Cla020347Watermelon (97103) v1cpiwmB040
CSPI01G03450Cla019006Watermelon (97103) v1cpiwmB100
CSPI01G03450Csa1G015780Cucumber (Chinese Long) v2cpicuB000
CSPI01G03450Csa5G608150Cucumber (Chinese Long) v2cpicuB038
CSPI01G03450MELO3C017273Melon (DHL92) v3.5.1cpimeB056
CSPI01G03450MELO3C012195Melon (DHL92) v3.5.1cpimeB006
CSPI01G03450ClCG06G015160Watermelon (Charleston Gray)cpiwcgB084
CSPI01G03450Lsi06G013640Bottle gourd (USVL1VR-Ls)cpilsiB071
CSPI01G03450Lsi04G003100Bottle gourd (USVL1VR-Ls)cpilsiB054
CSPI01G03450MELO3C017273.2Melon (DHL92) v3.6.1cpimedB050
CSPI01G03450MELO3C012195.2Melon (DHL92) v3.6.1cpimedB006
CSPI01G03450CsaV3_1G003480Cucumber (Chinese Long) v3cpicucB001
CSPI01G03450CsaV3_5G033780Cucumber (Chinese Long) v3cpicucB043
CSPI01G03450Cla97C05G105150Watermelon (97103) v2cpiwmbB064
CSPI01G03450Bhi02G000015Wax gourdcpiwgoB115
CSPI01G03450Cucsa.198740Cucumber (Gy14) v1cgycpiB310
CSPI01G03450Cucsa.284110Cucumber (Gy14) v1cgycpiB428
CSPI01G03450CmaCh04G015540Cucurbita maxima (Rimu)cmacpiB695
CSPI01G03450CmaCh10G002610Cucurbita maxima (Rimu)cmacpiB062
CSPI01G03450CmoCh11G002590Cucurbita moschata (Rifu)cmocpiB097
CSPI01G03450CmoCh10G002790Cucurbita moschata (Rifu)cmocpiB053
CSPI01G03450Cp4.1LG04g14140Cucurbita pepo (Zucchini)cpecpiB646
CSPI01G03450Cp4.1LG01g13290Cucurbita pepo (Zucchini)cpecpiB381
CSPI01G03450Cp4.1LG18g07230Cucurbita pepo (Zucchini)cpecpiB353
CSPI01G03450CsGy5G024260Cucumber (Gy14) v2cgybcpiB201
CSPI01G03450CsGy1G003410Cucumber (Gy14) v2cgybcpiB001
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CSPI01G03450CSPI05G24760Wild cucumber (PI 183967)cpicpiB030
The following block(s) are covering this gene:
GeneOrganismBlock
CSPI01G03450Wild cucumber (PI 183967)cpicpiB001
CSPI01G03450Cucurbita maxima (Rimu)cmacpiB086
CSPI01G03450Cucurbita maxima (Rimu)cmacpiB106
CSPI01G03450Cucurbita maxima (Rimu)cmacpiB223
CSPI01G03450Cucurbita maxima (Rimu)cmacpiB397
CSPI01G03450Cucurbita moschata (Rifu)cmocpiB079
CSPI01G03450Cucurbita moschata (Rifu)cmocpiB388
CSPI01G03450Cucumber (Chinese Long) v2cpicuB006
CSPI01G03450Watermelon (Charleston Gray)cpiwcgB061
CSPI01G03450Watermelon (97103) v1cpiwmB068
CSPI01G03450Cucurbita pepo (Zucchini)cpecpiB029
CSPI01G03450Cucurbita pepo (Zucchini)cpecpiB509
CSPI01G03450Bottle gourd (USVL1VR-Ls)cpilsiB025
CSPI01G03450Cucumber (Gy14) v2cgybcpiB005
CSPI01G03450Silver-seed gourdcarcpiB0419
CSPI01G03450Silver-seed gourdcarcpiB0497
CSPI01G03450Silver-seed gourdcarcpiB0550
CSPI01G03450Silver-seed gourdcarcpiB0627
CSPI01G03450Silver-seed gourdcarcpiB0628
CSPI01G03450Cucumber (Chinese Long) v3cpicucB004
CSPI01G03450Watermelon (97103) v2cpiwmbB057
CSPI01G03450Watermelon (97103) v2cpiwmbB080