Bhi11G000938 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi11G000938
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	Trypsin inhibitor 1
Location	chr11 : 33263031 .. 33263494 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATTAAGAGAGAAAAAAATGGAGTGGAAGAAAATTGGATTGGTGGCTATGGTGGGGATGCTGGTGATGGCTGCTTTGGTGGAGGAGTGTGCAGCCATTGAAGCACAATCTGTGGAAGGTTTGAATAAAATTGAATTCCCAAGAAAGATGATGAAGGAAGGAAGAATGTGCCCTAGAATCCTTGAGGAGTGCACTACTGATGATGATTGCATGAATGAGTGTATTTGCCTCCCCAATGGCTTTTGTGGTTGAAAGGAACCTCTCTTATAATATAATATAATATATACCTTAATCTATCTTCCATCTTTTGTGTCTTTGTGTGTTATTCTTTGTCTATTTCTATGGTAATAAAGTTTGTCTAATGTATTTGATCAATTTGTGGAACACCCATCAAGTAATGATATGTAACCTTTCCAATTTCTATGTGTATTTGATTCCTCTATATTTCCTTTCAATGCTCTTAAC

mRNA sequence

CATTAAGAGAGAAAAAAATGGAGTGGAAGAAAATTGGATTGGTGGCTATGGTGGGGATGCTGGTGATGGCTGCTTTGGTGGAGGAGTGTGCAGCCATTGAAGCACAATCTGTGGAAGGTTTGAATAAAATTGAATTCCCAAGAAAGATGATGAAGGAAGGAAGAATGTGCCCTAGAATCCTTGAGGAGTGCACTACTGATGATGATTGCATGAATGAGTGTATTTGCCTCCCCAATGGCTTTTGTGGTTGAAAGGAACCTCTCTTATAATATAATATAATATATACCTTAATCTATCTTCCATCTTTTGTGTCTTTGTGTGTTATTCTTTGTCTATTTCTATGGTAATAAAGTTTGTCTAATGTATTTGATCAATTTGTGGAACACCCATCAAGTAATGATATGTAACCTTTCCAATTTCTATGTGTATTTGATTCCTCTATATTTCCTTTCAATGCTCTTAAC

Coding sequence (CDS)

ATGGAGTGGAAGAAAATTGGATTGGTGGCTATGGTGGGGATGCTGGTGATGGCTGCTTTGGTGGAGGAGTGTGCAGCCATTGAAGCACAATCTGTGGAAGGTTTGAATAAAATTGAATTCCCAAGAAAGATGATGAAGGAAGGAAGAATGTGCCCTAGAATCCTTGAGGAGTGCACTACTGATGATGATTGCATGAATGAGTGTATTTGCCTCCCCAATGGCTTTTGTGGTTGA

Protein sequence

MEWKKIGLVAMVGMLVMAALVEECAAIEAQSVEGLNKIEFPRKMMKEGRMCPRILEECTTDDDCMNECICLPNGFCG

Homology

[Close]

BLAST of Bhi11G000938 vs. Swiss-Prot
Match: sp|P34950|ITR5_LUFAE (Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 2.2e-08
Identity = 28/63 (44.44%), Postives = 38/63 (60.32%), Query Frame = 0


Query: 17 MAALVEECAAIEAQSVEGLNKIEFPRKMM--KEGRMCPRILEECTTDDDCMNECICLPNG 76
          MA++ E    +E   +      + PRK+M  + G +CPRIL  C TDDDCM +C CL NG
Sbjct: 1  MASVAESSGVVEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLSNG 60

Query: 77 FCG 78
          +CG
Sbjct: 61 YCG 63

[Close]

BLAST of Bhi11G000938 vs. Swiss-Prot
Match: sp|Q9S8I2|ITR3_LUFAE (Trypsin inhibitor 3 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 3.1e-07
Identity = 21/29 (72.41%), Postives = 25/29 (86.21%), Query Frame = 0


Query: 49 RMCPRILEECTTDDDCMNECICLPNGFCG 78
          R+CPRIL EC++D DC+ ECICL NGFCG
Sbjct: 1  RICPRILMECSSDSDCLAECICLENGFCG 29

[Close]

BLAST of Bhi11G000938 vs. Swiss-Prot
Match: sp|P25849|ITR1_LUFAE (Trypsin inhibitor 1 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 1.6e-06
Identity = 20/29 (68.97%), Postives = 24/29 (82.76%), Query Frame = 0


Query: 49 RMCPRILEECTTDDDCMNECICLPNGFCG 78
          R+CPRIL EC++D DC+ ECICL  GFCG
Sbjct: 1  RICPRILMECSSDSDCLAECICLEQGFCG 29

[Close]

BLAST of Bhi11G000938 vs. Swiss-Prot
Match: sp|P35628|ITR4_LUFAE (Trypsin inhibitor 4 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 5.9e-06
Identity = 19/28 (67.86%), Postives = 23/28 (82.14%), Query Frame = 0


Query: 50 MCPRILEECTTDDDCMNECICLPNGFCG 78
          +CPRIL  C++D DC+ ECICL NGFCG
Sbjct: 1  ICPRILMPCSSDSDCLAECICLENGFCG 28

[Close]

BLAST of Bhi11G000938 vs. Swiss-Prot
Match: sp|P10293|ITR3_CUCPE (Trypsin inhibitor 3 OS=Cucurbita pepo OX=3663 PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 7.7e-06
Identity = 19/31 (61.29%), Postives = 24/31 (77.42%), Query Frame = 0


Query: 47 EGRMCPRILEECTTDDDCMNECICLPNGFCG 78
          E R+CP+IL EC  D DC+ ECICL +G+CG
Sbjct: 2  EERVCPKILMECKKDSDCLAECICLEHGYCG 32

[Close]

BLAST of Bhi11G000938 vs. TrEMBL
Match: tr|A0A0A0KT01|A0A0A0KT01_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000805 PE=3 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 2.6e-27
Identity = 64/79 (81.01%), Postives = 73/79 (92.41%), Query Frame = 0


Query: 1  MEWKKIGLVAMVGMLVMAALVEEC-AAIEAQSVEGLNKIEFPRKMM-KEGRMCPRILEEC 60
          MEWKKIG++A+VGMLVMAALVE+C AAIE++SV+GLNK E PRKMM +E RMCPRILEEC
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC 60

Query: 61 TTDDDCMNECICLPNGFCG 78
          TTDDDCMN+CICL NGFCG
Sbjct: 61 TTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of Bhi11G000938 vs. TrEMBL
Match: tr|A0A0A0KY34|A0A0A0KY34_CUCSA (Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.0e-07
Identity = 36/83 (43.37%), Postives = 50/83 (60.24%), Query Frame = 0


Query: 1  MEWKKIGLVAMVGMLVMAALVEECA-AIEAQSV----EGLNKIEFPRKMMKE-GRMCPRI 60
          MEWKKI LVAM+GML+MA   E     ++ + +    +G+N  E+ + + K+    CPRI
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVN--EYSQNIGKDTAPGCPRI 60

Query: 61 LEECTTDDDCMNECICLPNGFCG 78
          L +C TD DC   C C P G+CG
Sbjct: 61 LMKCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of Bhi11G000938 vs. NCBI nr
Match: KGN52760.1 (hypothetical protein Csa_4G000805 [Cucumis sativus])

HSP 1 Score: 129.8 bits (325), Expect = 4.0e-27
Identity = 64/79 (81.01%), Postives = 73/79 (92.41%), Query Frame = 0


Query: 1  MEWKKIGLVAMVGMLVMAALVEEC-AAIEAQSVEGLNKIEFPRKMM-KEGRMCPRILEEC 60
          MEWKKIG++A+VGMLVMAALVE+C AAIE++SV+GLNK E PRKMM +E RMCPRILEEC
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC 60

Query: 61 TTDDDCMNECICLPNGFCG 78
          TTDDDCMN+CICL NGFCG
Sbjct: 61 TTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of Bhi11G000938 vs. NCBI nr
Match: XP_011654311.1 (PREDICTED: uncharacterized protein LOC105435333 [Cucumis sativus])

HSP 1 Score: 129.8 bits (325), Expect = 4.0e-27
Identity = 64/79 (81.01%), Postives = 73/79 (92.41%), Query Frame = 0


Query: 1   MEWKKIGLVAMVGMLVMAALVEEC-AAIEAQSVEGLNKIEFPRKMM-KEGRMCPRILEEC 60
           MEWKKIG++A+VGMLVMAALVE+C AAIE++SV+GLNK E PRKMM +E RMCPRILEEC
Sbjct: 126 MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMNEETRMCPRILEEC 185

Query: 61  TTDDDCMNECICLPNGFCG 78
           TTDDDCMN+CICL NGFCG
Sbjct: 186 TTDDDCMNDCICLSNGFCG 204

[Close]

BLAST of Bhi11G000938 vs. NCBI nr
Match: XP_022984819.1 (trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 82.0 bits (201), Expect = 9.5e-13
Identity = 42/81 (51.85%), Postives = 53/81 (65.43%), Query Frame = 0


Query: 1  MEWKKIGLVAMVGMLVMAALVEECAAIEAQSV----EGLNKIEFPRKMMKEGRMCPRILE 60
          MEWK+  L+AMVG+L+MAA  E  A    + +    +G+N  +FPRKMM   + CPRIL 
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVN--DFPRKMMNGVQGCPRILM 60

Query: 61 ECTTDDDCMNECICLPNGFCG 78
          EC  D DC+  CIC PNGFCG
Sbjct: 61 ECELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of Bhi11G000938 vs. NCBI nr
Match: XP_023552845.1 (uncharacterized protein LOC111810370 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 81.3 bits (199), Expect = 1.6e-12
Identity = 40/81 (49.38%), Postives = 52/81 (64.20%), Query Frame = 0


Query: 1   MEWKKIGLVAMVGMLVMAALVEECAAIEAQSV----EGLNKIEFPRKMMKEGRMCPRILE 60
           MEWK+  L+AMVG+L+MAA  +  A    + +    +G+N  +FPRKMM     CPRIL 
Sbjct: 85  MEWKRGALIAMVGLLLMAAFADSAAVHGGEVIQLVSDGVN--DFPRKMMNAALGCPRILM 144

Query: 61  ECTTDDDCMNECICLPNGFCG 78
           EC  D DC+  C+C PNGFCG
Sbjct: 145 ECEVDSDCLPGCVCRPNGFCG 163

[Close]

BLAST of Bhi11G000938 vs. NCBI nr
Match: KGN52761.1 (Trypsin inhibitor 1 [Cucumis sativus])

HSP 1 Score: 63.2 bits (152), Expect = 4.6e-07
Identity = 36/83 (43.37%), Postives = 50/83 (60.24%), Query Frame = 0


Query: 1  MEWKKIGLVAMVGMLVMAALVEECA-AIEAQSV----EGLNKIEFPRKMMKE-GRMCPRI 60
          MEWKKI LVAM+GML+MA   E     ++ + +    +G+N  E+ + + K+    CPRI
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVN--EYSQNIGKDTAPGCPRI 60

Query: 61 LEECTTDDDCMNECICLPNGFCG 78
          L +C TD DC   C C P G+CG
Sbjct: 61 LMKCKTDSDCYPGCTCKPMGYCG 81

The following BLAST results are available for this feature:

BLAST of Bhi11G000938 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of Bhi11G000938 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|P34950\|ITR5_LUFAE	2.2e-08	44.44	Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
sp\|Q9S8I2\|ITR3_LUFAE	3.1e-07	72.41	Trypsin inhibitor 3 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
sp\|P25849\|ITR1_LUFAE	1.6e-06	68.97	Trypsin inhibitor 1 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
sp\|P35628\|ITR4_LUFAE	5.9e-06	67.86	Trypsin inhibitor 4 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
sp\|P10293\|ITR3_CUCPE	7.7e-06	61.29	Trypsin inhibitor 3 OS=Cucurbita pepo OX=3663 PE=1 SV=1	[more]

BLAST of Bhi11G000938 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KT01\|A0A0A0KT01_CUCSA	2.6e-27	81.01	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000805 PE=3 SV=1	[more]
tr\|A0A0A0KY34\|A0A0A0KY34_CUCSA	3.0e-07	43.37	Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1	[more]

BLAST of Bhi11G000938 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN52760.1	4.0e-27	81.01	hypothetical protein Csa_4G000805 [Cucumis sativus]	[more]
XP_011654311.1	4.0e-27	81.01	PREDICTED: uncharacterized protein LOC105435333 [Cucumis sativus]	[more]
XP_022984819.1	9.5e-13	51.85	trypsin inhibitor 1-like [Cucurbita maxima]	[more]
XP_023552845.1	1.6e-12	49.38	uncharacterized protein LOC111810370 [Cucurbita pepo subsp. pepo]	[more]
KGN52761.1	4.6e-07	43.37	Trypsin inhibitor 1 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004866	endopeptidase inhibitor activity
GO:0004867	serine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR011052	Proteinase_amylase_inhib_sf
IPR000737	Prot_inh_squash

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi11M000938	Bhi11M000938	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00286	PTI_1	coord: 49..77 e-value: 1.2E-11 score: 54.7
None	No IPR available	GENE3D	G3DSA:3.10.450.30		coord: 36..77 e-value: 3.9E-13 score: 51.6
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 49..77 e-value: 2.1E-14 score: 53.1
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 51..70
IPR000737	Proteinase inhibitor I7, squash	CDD	cd00150	PlantTI	coord: 51..77 e-value: 2.76512E-9 score: 46.9871
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	SSF57027	Plant inhibitors of proteinases and amylases	coord: 47..77

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi11G000938	Cucumber (Gy14) v1	cgywgoB443
Bhi11G000938	Cucumber (Gy14) v2	cgybwgoB342
Bhi11G000938	Cucumber (Gy14) v2	cgybwgoB619
Bhi11G000938	Cucurbita maxima (Rimu)	cmawgoB0410
Bhi11G000938	Cucurbita maxima (Rimu)	cmawgoB0634
Bhi11G000938	Cucurbita maxima (Rimu)	cmawgoB0814
Bhi11G000938	Cucurbita moschata (Rifu)	cmowgoB0405
Bhi11G000938	Cucurbita moschata (Rifu)	cmowgoB0635
Bhi11G000938	Cucurbita moschata (Rifu)	cmowgoB0810
Bhi11G000938	Cucurbita pepo (Zucchini)	cpewgoB0266
Bhi11G000938	Cucurbita pepo (Zucchini)	cpewgoB0311
Bhi11G000938	Cucurbita pepo (Zucchini)	cpewgoB0477
Bhi11G000938	Wild cucumber (PI 183967)	cpiwgoB358
Bhi11G000938	Wild cucumber (PI 183967)	cpiwgoB659
Bhi11G000938	Cucumber (Chinese Long) v3	cucwgoB360
Bhi11G000938	Cucumber (Chinese Long) v3	cucwgoB674
Bhi11G000938	Cucumber (Chinese Long) v2	cuwgoB356
Bhi11G000938	Cucumber (Chinese Long) v2	cuwgoB651
Bhi11G000938	Bottle gourd (USVL1VR-Ls)	lsiwgoB292
Bhi11G000938	Bottle gourd (USVL1VR-Ls)	lsiwgoB307
Bhi11G000938	Melon (DHL92) v3.6.1	medwgoB200
Bhi11G000938	Melon (DHL92) v3.6.1	medwgoB569
Bhi11G000938	Melon (DHL92) v3.5.1	mewgoB198
Bhi11G000938	Melon (DHL92) v3.5.1	mewgoB564
Bhi11G000938	Watermelon (Charleston Gray)	wcgwgoB078
Bhi11G000938	Watermelon (Charleston Gray)	wcgwgoB259
Bhi11G000938	Watermelon (97103) v2	wgowmbB001
Bhi11G000938	Watermelon (97103) v2	wgowmbB033
Bhi11G000938	Watermelon (97103) v1	wgowmB007
Bhi11G000938	Silver-seed gourd	carwgoB0381
Bhi11G000938	Silver-seed gourd	carwgoB0456