Bhi10G001408 (gene) Wax gourd

NameBhi10G001408
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionCopper-transporting ATPase
Locationchr10 : 44813209 .. 44815256 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTAAGGAAAGTATTGGTTTACATACTTACTTTTGTCCCTGTACTTTTAAAATTTGTCCACTTTAACCCATTCATTTTCATTTTTGTTTCAATGTTAAGATGTTAAAATAGTCATTTCTTAGAAAGTACAAAGAAATGGACTTTTTAAAAATACAAGACTAAAATGAATCAAAATGTAAAACACAAATATCAAAATGAACTAAAGTTGAAAGTACAGGGACTGAAGTAGTAGTATTAAGCCGAAAATATTTGTGGAGTATTTTTAGCTTAAATATGTCACGAGAAACACTTAAAAAAAAAAAAAAAAATTAAGAGCACATTACCCTAGATTATGGAATTGATCAACTTTTTAGTTGGATGAATATTGAATTATAAGTTTTCATTTCTTTTATGACCTTACTTTATAGCAGGATTTGTGCTATAGGTTGTTTAATTGTGTTCTTGAGTCTTAAAAAAATTACAGTTTTTTTTTCTTTCCTTTCAACCAGGTGAAAACGGTAGTTTTTGACAAGACTGGAACTCTAACGGTTGGCAGGCCGCAGGTAGTTAGTGTTGTGCTTTTTTCTACAGTTCCAATGCAGGAGCTCTGTGATGCAGCAATAGCAATTGAGGTGAGTGCATTTGTAATTCAAGTTGATAGGTTTTGAATCTTGTATTCAGTTTTGTTTAGAAGTTTCTTATGTAAATTGAAGGTATTTTTGTGTGCTTTTTCATGAAAATTTGTACTTTTTCATGCTAGTAACATGTACACCCTTCGAAATTAAAAGTAAAAATTGAGATCTTACTTTTGTGTAACACTTTGAGTATAGTCAAACAGCAAACACCCTATAGCAAAATCTGTAGTGGAGCATGCGAATAAAATGAGAAGGAAGTTCGGGGCCCGAAGCGAATGTCGCAAACGCATCCAGAACTTCGAGGTGTTCCCGGGAGGAGGGGTTGGAGGAAGAATTGATAGAAAGACAGTTTTAGTAGGAAACCGAAGGCTCATGCGAGCTCACAGCGTTCCTGTCAGCCCTCAAGTTGATAGGTACGCTATAGAAAACGAGCGACTGGCTCAAACATGCGTCTTGGTGGCTATTAATGGCAAGATAGCAGGAGGTTTTGGCGTTATAGATGCACCAAAGCCTGGGACTAAAGCTGTCATATCATTTCTTCGCTCGATTGGCATCTCAACCATATTGGTCACTGGAGATAACTGGGCCACAGCCTTCGCCGTTGCAAGAGGAGTTGGGATTAATGAAGTCTTTGCAGAGATGGATCCAATTGGAAAAGCCAACAAGATTAAATCCTTCAAGGTAAAACTTTAGTTCACATTTGTTAACCATTTTGTTTTTGAAATTTATGCTTGTTTTCTCCTAATTTATTTATCATGATTTTCACATTCCGTAGAGAAACATTTGAATTCTTAGTCAAAATCTAAACACAAAAACTACTTTTTTCTAGTTTTCAAAACTTAATTTGATTTTTGTGAACATAGGTAGAAAGTATACTACACAACATAGAAAGTTAGGTGTGAACGTAATTTTCAAAAACCAAAAACAAAAAACAAAATAGTTACTAAATGAAGTCTTCGTTTCTAGTGCTTTGCTTGCTGCTCCATATAGCAAATTTTATGTTTATGAAATGACTCATCCTTTGTTGAGTTGAACGCAGTCGACTGGGAATATTGTGGCAATGGTGGGAGATGGAGTAAATGACTCACATGCCTTAGCTGCAGCTGATGTTGGTATTGCAATTGGTGCTGGAACAAATATTGCTATAGAAGCAGCTGATATTGTTCTAATGCGAAGCAATTTAGAAGATGTAGTTACTGCGATAGATCTCTCAAGACAAACTGTTTATCGTATATGGCTTAACTACATCTGGGCTCTTGGCTATAATATCATCGGCATGCCTATCGCTGCCGGAATTCTATATCCCTTCTTTGGAATCCGATTGCCACCGTGGCTTGCCGGTGCCTGCATGGCTGCTTCCTCTCTTAGTGTTGTATGTTCTTCTCTCTTATTGAAATGTTATAGGAGACCCCTGAATTTTCAATCTGCTTGA

mRNA sequence

ATGGTGAAAACGGTAGTTTTTGACAAGACTGGAACTCTAACGGTTGGCAGGCCGCAGGTAGTTAGTGTTGTGCTTTTTTCTACAGTTCCAATGCAGGAGCTCTGTGATGCAGCAATAGCAATTGAGTCAAACAGCAAACACCCTATAGCAAAATCTGTAGTGGAGCATGCGAATAAAATGAGAAGGAAGTTCGGGGCCCGAAGCGAATGTCGCAAACGCATCCAGAACTTCGAGGTGTTCCCGGGAGGAGGGGTTGGAGGAAGAATTGATAGAAAGACAGTTTTAGTAGGAAACCGAAGGCTCATGCGAGCTCACAGCGTTCCTGTCAGCCCTCAAGTTGATAGGTACGCTATAGAAAACGAGCGACTGGCTCAAACATGCGTCTTGGTGGCTATTAATGGCAAGATAGCAGGAGGTTTTGGCGTTATAGATGCACCAAAGCCTGGGACTAAAGCTGTCATATCATTTCTTCGCTCGATTGGCATCTCAACCATATTGGTCACTGGAGATAACTGGGCCACAGCCTTCGCCGTTGCAAGAGGAGTTGGGATTAATGAAGTCTTTGCAGAGATGGATCCAATTGGAAAAGCCAACAAGATTAAATCCTTCAAGTCGACTGGGAATATTGTGGCAATGGTGGGAGATGGAGTAAATGACTCACATGCCTTAGCTGCAGCTGATGTTGGTATTGCAATTGGTGCTGGAACAAATATTGCTATAGAAGCAGCTGATATTGTTCTAATGCGAAGCAATTTAGAAGATGTAGTTACTGCGATAGATCTCTCAAGACAAACTGTTTATCGTATATGGCTTAACTACATCTGGGCTCTTGGCTATAATATCATCGGCATGCCTATCGCTGCCGGAATTCTATATCCCTTCTTTGGAATCCGATTGCCACCGTGGCTTGCCGGTGCCTGCATGGCTGCTTCCTCTCTTAGTGTTGTATGTTCTTCTCTCTTATTGAAATGTTATAGGAGACCCCTGAATTTTCAATCTGCTTGA

Coding sequence (CDS)

ATGGTGAAAACGGTAGTTTTTGACAAGACTGGAACTCTAACGGTTGGCAGGCCGCAGGTAGTTAGTGTTGTGCTTTTTTCTACAGTTCCAATGCAGGAGCTCTGTGATGCAGCAATAGCAATTGAGTCAAACAGCAAACACCCTATAGCAAAATCTGTAGTGGAGCATGCGAATAAAATGAGAAGGAAGTTCGGGGCCCGAAGCGAATGTCGCAAACGCATCCAGAACTTCGAGGTGTTCCCGGGAGGAGGGGTTGGAGGAAGAATTGATAGAAAGACAGTTTTAGTAGGAAACCGAAGGCTCATGCGAGCTCACAGCGTTCCTGTCAGCCCTCAAGTTGATAGGTACGCTATAGAAAACGAGCGACTGGCTCAAACATGCGTCTTGGTGGCTATTAATGGCAAGATAGCAGGAGGTTTTGGCGTTATAGATGCACCAAAGCCTGGGACTAAAGCTGTCATATCATTTCTTCGCTCGATTGGCATCTCAACCATATTGGTCACTGGAGATAACTGGGCCACAGCCTTCGCCGTTGCAAGAGGAGTTGGGATTAATGAAGTCTTTGCAGAGATGGATCCAATTGGAAAAGCCAACAAGATTAAATCCTTCAAGTCGACTGGGAATATTGTGGCAATGGTGGGAGATGGAGTAAATGACTCACATGCCTTAGCTGCAGCTGATGTTGGTATTGCAATTGGTGCTGGAACAAATATTGCTATAGAAGCAGCTGATATTGTTCTAATGCGAAGCAATTTAGAAGATGTAGTTACTGCGATAGATCTCTCAAGACAAACTGTTTATCGTATATGGCTTAACTACATCTGGGCTCTTGGCTATAATATCATCGGCATGCCTATCGCTGCCGGAATTCTATATCCCTTCTTTGGAATCCGATTGCCACCGTGGCTTGCCGGTGCCTGCATGGCTGCTTCCTCTCTTAGTGTTGTATGTTCTTCTCTCTTATTGAAATGTTATAGGAGACCCCTGAATTTTCAATCTGCTTGA

Protein sequence

MVKTVVFDKTGTLTVGRPQVVSVVLFSTVPMQELCDAAIAIESNSKHPIAKSVVEHANKMRRKFGARSECRKRIQNFEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVPVSPQVDRYAIENERLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILVTGDNWATAFAVARGVGINEVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIEAADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPPWLAGACMAASSLSVVCSSLLLKCYRRPLNFQSA
BLAST of Bhi10G001408 vs. TAIR10
Match: AT1G63440.1 (heavy metal atpase 5)

HSP 1 Score: 327.0 bits (837), Expect = 1.3e-89
Identity = 173/327 (52.91%), Postives = 229/327 (70.03%), Query Frame = 0

Query: 2   VKTVVFDKTGTLTVGRPQVVSVVLFSTVPMQELCDAAIAIESNSKHPIAKSVVEHANKMR 61
           V  +VFDKTGTLT+G+P VV   L   + ++E  +   A E NS+HP+AK++VE+A K R
Sbjct: 655 VNCIVFDKTGTLTMGKPVVVKTKLLKNMVLREFYELVAATEVNSEHPLAKAIVEYAKKFR 714

Query: 62  RKFGARSECRKRIQNFEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVPVSPQVDRYAIENE 121
                 +       +F    G GV   +  + ++VGN+ LM  H V +    +    ++E
Sbjct: 715 D--DEENPAWPEACDFVSITGKGVKATVKGREIMVGNKNLMNDHKVIIPDDAEELLADSE 774

Query: 122 RLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILVTGDNWATAFAVARG 181
            +AQT +LV+IN ++ G   V D  KP  +  IS L+S+ I +I+VTGDNW TA ++AR 
Sbjct: 775 DMAQTGILVSINSELIGVLSVSDPLKPSAREAISILKSMNIKSIMVTGDNWGTANSIARE 834

Query: 182 VGINEVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 241
           VGI+ V AE  P  KA K+K  ++ G++VAMVGDG+NDS AL AADVG+AIGAGT+IAIE
Sbjct: 835 VGIDSVIAEAKPEQKAEKVKELQAAGHVVAMVGDGINDSPALVAADVGMAIGAGTDIAIE 894

Query: 242 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP 301
           AADIVLM+SNLEDV+TAIDLSR+T  RI LNY+WALGYN++G+PIAAG+L+P    RLPP
Sbjct: 895 AADIVLMKSNLEDVITAIDLSRKTFSRIRLNYVWALGYNLMGIPIAAGVLFPGTRFRLPP 954

Query: 302 WLAGACMAASSLSVVCSSLLLKCYRRP 329
           W+AGA MAASS+SVVC SLLLK Y+RP
Sbjct: 955 WIAGAAMAASSVSVVCCSLLLKNYKRP 979

BLAST of Bhi10G001408 vs. TAIR10
Match: AT5G44790.1 (copper-exporting ATPase / responsive-to-antagonist 1 / copper-transporting ATPase (RAN1))

HSP 1 Score: 303.9 bits (777), Expect = 1.2e-82
Identity = 166/340 (48.82%), Postives = 231/340 (67.94%), Query Frame = 0

Query: 2   VKTVVFDKTGTLTVGRPQVVSVVLFSTVPMQELCDAAIAIESNSKHPIAKSVVEHANKMR 61
           VK V+FDKTGTLT G+  V +  +FS +   E      + E++S+HP+AK++V +A    
Sbjct: 652 VKYVIFDKTGTLTQGKATVTTTKVFSEMDRGEFLTLVASAEASSEHPLAKAIVAYARHF- 711

Query: 62  RKFGARSE----CRKRIQN---------FEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVP 121
             F   +E      K +QN         F   PG G+   ++ K +LVGNR+LM  +++ 
Sbjct: 712 HFFDESTEDGETNNKDLQNSGWLLDTSDFSALPGKGIQCLVNEKMILVGNRKLMSENAIN 771

Query: 122 VSPQVDRYAIENERLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILVT 181
           +   V+++  + E   +T V+VA NGK+ G  G+ D  K     V+  L  +G+  I+VT
Sbjct: 772 IPDHVEKFVEDLEESGKTGVIVAYNGKLVGVMGIADPLKREAALVVEGLLRMGVRPIMVT 831

Query: 182 GDNWATAFAVARGVGINEVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAADV 241
           GDNW TA AVA+ VGI +V AE+ P GKA+ I+S +  G+ VAMVGDG+NDS ALAAADV
Sbjct: 832 GDNWRTARAVAKEVGIEDVRAEVMPAGKADVIRSLQKDGSTVAMVGDGINDSPALAAADV 891

Query: 242 GIAIGAGTNIAIEAADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAA 301
           G+AIGAGT++AIEAAD VLMR+NLEDV+TAIDLSR+T+ RI LNY++A+ YN++ +PIAA
Sbjct: 892 GMAIGAGTDVAIEAADYVLMRNNLEDVITAIDLSRKTLTRIRLNYVFAMAYNVVSIPIAA 951

Query: 302 GILYPFFGIRLPPWLAGACMAASSLSVVCSSLLLKCYRRP 329
           G+ +P   ++LPPW AGACMA SS+SVVCSSLLL+ Y++P
Sbjct: 952 GVFFPVLRVQLPPWAAGACMALSSVSVVCSSLLLRRYKKP 990

BLAST of Bhi10G001408 vs. TAIR10
Match: AT4G33520.2 (P-type ATP-ase 1)

HSP 1 Score: 179.1 bits (453), Expect = 4.5e-45
Identity = 123/335 (36.72%), Postives = 177/335 (52.84%), Query Frame = 0

Query: 1   MVKTVVFDKTGTLTVGRPQVVSVV--------LFSTVPMQELCDAAIAIESNSKHPIAKS 60
           +V TVVFDKTGTLT G P V  V+        L  T    E+   A A+ESN+ HP+ K+
Sbjct: 591 LVDTVVFDKTGTLTKGHPVVTEVIIPENPRHNLNDTWSEVEVLMLAAAVESNTTHPVGKA 650

Query: 61  VVEHANKMRRKFGARSECRKRIQNFEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVPVSP- 120
           +V+ A     +       +     F   PG G    ++ K V VG    ++ H    +  
Sbjct: 651 IVKAARARNCQ-----TMKAEDGTFTEEPGSGAVAIVNNKRVTVGTLEWVKRHGATGNSL 710

Query: 121 -QVDRYAIENERLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILVTGD 180
             ++ + I N    Q+ V + ++  +A      D  +     V+  L   GI   +++GD
Sbjct: 711 LALEEHEINN----QSVVYIGVDNTLAAVIRFEDKVREDAAQVVENLTRQGIDVYMLSGD 770

Query: 181 NWATAFAVARGVGIN--EVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAADV 240
               A  VA  VGIN   V A + P  K N I   +    IVAMVGDG+ND+ ALA+++V
Sbjct: 771 KRNAANYVASVVGINHERVIAGVKPAEKKNFINELQKNKKIVAMVGDGINDAAALASSNV 830

Query: 241 GIAIGAGTNIAIEAADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAA 300
           G+A+G G   A E + +VLM + L  ++ A++LSRQT+  +  N  WA GYNI+G+PIAA
Sbjct: 831 GVAMGGGAGAASEVSPVVLMGNRLTQLLDAMELSRQTMKTVKQNLWWAFGYNIVGIPIAA 890

Query: 301 GILYPFFGIRLPPWLAGACMAASSLSVVCSSLLLK 324
           G+L P  G  L P +AGA M  SSL V+ +SLLL+
Sbjct: 891 GVLLPLTGTMLTPSMAGALMGVSSLGVMTNSLLLR 916

BLAST of Bhi10G001408 vs. TAIR10
Match: AT5G21930.1 (P-type ATPase of Arabidopsis 2)

HSP 1 Score: 169.9 bits (429), Expect = 2.7e-42
Identity = 122/342 (35.67%), Postives = 182/342 (53.22%), Query Frame = 0

Query: 2   VKTVVFDKTGTLTVGRPQVVSVVLFSTVPMQELCDAAIAIESNSKHPIAKSVVEHANKMR 61
           +  V  DKTGTLT GRP VVS V       QE+   A A+E  + HPIAK++V  A  + 
Sbjct: 542 IDCVALDKTGTLTEGRP-VVSGVASLGYEEQEVLKMAAAVEKTATHPIAKAIVNEAESLN 601

Query: 62  RKFGARSECRKRIQNFEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVPVSPQVDRYAIEN- 121
            K     E R ++      PG G    ID + V VG+   +    +  +   D   +E+ 
Sbjct: 602 LK---TPETRGQLTE----PGFGTLAEIDGRFVAVGSLEWVSDRFLKKNDSSDMVKLESL 661

Query: 122 -----------ERLAQTCVLVAINGK-IAGGFGVIDAPKPGTKAVISFLRSIGISTILVT 181
                       R ++T V V   G+ I G   + D  +   +  ++ L+  GI T+L++
Sbjct: 662 LDHKLSNTSSTSRYSKTVVYVGREGEGIIGAIAISDCLRQDAEFTVARLQEKGIKTVLLS 721

Query: 182 GDNWATAFAVARGVGINEVFA--EMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAA 241
           GD       VA+ VGI        + P  K   I + +S+G+ VAMVGDG+ND+ +LA A
Sbjct: 722 GDREGAVATVAKNVGIKSESTNYSLSPEKKFEFISNLQSSGHRVAMVGDGINDAPSLAQA 781

Query: 242 DVGIA--IGAGTNIAIEAADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGM 301
           DVGIA  I A  N A  AA ++L+R+ L  VV A+ L++ T+ +++ N  WA+ YN+I +
Sbjct: 782 DVGIALKIEAQENAASNAASVILVRNKLSHVVDALSLAQATMSKVYQNLAWAIAYNVISI 841

Query: 302 PIAAGILYPFFGIRLPPWLAGACMAASSLSVVCSSLLLKCYR 327
           PIAAG+L P +   + P L+G  MA SS+ VV +SLLL+ ++
Sbjct: 842 PIAAGVLLPQYDFAMTPSLSGGLMALSSIFVVSNSLLLQLHK 875

BLAST of Bhi10G001408 vs. TAIR10
Match: AT2G19110.1 (heavy metal atpase 4)

HSP 1 Score: 136.0 bits (341), Expect = 4.4e-32
Identity = 102/339 (30.09%), Postives = 178/339 (52.51%), Query Frame = 0

Query: 2   VKTVVFDKTGTLTVGRPQVVSVVLFS-TVPMQELCDAAIAIESNSKHPIAKSVVEHANKM 61
           +K V FDKTGT+T G   V+     S  + ++ L     ++ES S HP+A ++V++A  +
Sbjct: 395 IKIVAFDKTGTITRGEFIVIDFKSLSRDINLRSLLYWVSSVESKSSHPMAATIVDYAKSV 454

Query: 62  RRKFGARSECRKRIQNFEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVPVSPQVDRYAIEN 121
                 R E    +++++ FPG G+ G+ID   + +GN+++         P+++     +
Sbjct: 455 --SVEPRPE---EVEDYQNFPGEGIYGKIDGNDIFIGNKKIASRAGCSTVPEIE----VD 514

Query: 122 ERLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILVTGDNWATAFAVAR 181
            +  +T   V +  ++AG F + DA + G    ++ L+S+GI T ++TGDN A A     
Sbjct: 515 TKGGKTVGYVYVGERLAGFFNLSDACRSGVSQAMAELKSLGIKTAMLTGDNQAAAMHAQE 574

Query: 182 GVG--INEVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAADVGIAIG-AGTN 241
            +G  ++ V  ++ P  K+  I+ FK  G   AMVGDGVND+ ALA AD+GI++G +G+ 
Sbjct: 575 QLGNVLDVVHGDLLPEDKSRIIQEFKKEGP-TAMVGDGVNDAPALATADIGISMGISGSA 634

Query: 242 IAIEAADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGI 301
           +A +  +I+LM +++  +  A+ L+R+   ++  N         + + + AGIL   F  
Sbjct: 635 LATQTGNIILMSNDIRRIPQAVKLARRARRKVVENV-------CLSIILKAGILALAFAG 694

Query: 302 RLPPWLAGACMAASSLSVVCSSLLL----------KCYR 327
               W A      + L V+ +S+LL          KCYR
Sbjct: 695 HPLIWAAVLVDVGTCLLVIFNSMLLLREKKKIGNKKCYR 716

BLAST of Bhi10G001408 vs. Swiss-Prot
Match: sp|Q6H7M3|HMA4_ORYSJ (Copper-transporting ATPase HMA4 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA4 PE=1 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 4.8e-113
Identity = 203/331 (61.33%), Postives = 263/331 (79.46%), Query Frame = 0

Query: 2   VKTVVFDKTGTLTVGRPQVVSVVLFSTVPMQELCDAAIAIESNSKHPIAKSVVEHANKMR 61
           VK ++FDKTGTLTVG+P VV   +FS +P+ ELCD A   E+NS+HP++K++VE+  K+R
Sbjct: 635 VKAIIFDKTGTLTVGKPSVVQTKVFSKIPLLELCDLAAGAEANSEHPLSKAIVEYTKKLR 694

Query: 62  RKFGARSECRKRIQNFEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVPVSPQVDRYAIENE 121
            ++G+ S+     ++FEV PG GV   ++ K VLVGN+RLM+   VP+S +V+ +  E E
Sbjct: 695 EQYGSHSDHIMESKDFEVHPGAGVSANVEGKLVLVGNKRLMQEFEVPISSEVEGHMSETE 754

Query: 122 RLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILVTGDNWATAFAVARG 181
            LA+TCVLVAI+  I G   V D  KP     IS+L S+GIS+I+VTGDNWATA ++A+ 
Sbjct: 755 ELARTCVLVAIDRTICGALSVSDPLKPEAGRAISYLSSMGISSIMVTGDNWATAKSIAKE 814

Query: 182 VGINEVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 241
           VGI  VFAE+DP+GKA KIK  +  G  VAMVGDG+NDS ALAAADVG+AIGAGT++AIE
Sbjct: 815 VGIGTVFAEIDPVGKAEKIKDLQMKGLTVAMVGDGINDSPALAAADVGLAIGAGTDVAIE 874

Query: 242 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP 301
           AADIVLMRS+LEDV+TAIDLSR+T+ RI LNY+WALGYN++GMP+AAG+L+PF GIRLPP
Sbjct: 875 AADIVLMRSSLEDVITAIDLSRKTLSRIRLNYVWALGYNVLGMPVAAGVLFPFTGIRLPP 934

Query: 302 WLAGACMAASSLSVVCSSLLLKCYRRPLNFQ 333
           WLAGACMAASS+SVVCSSLLL+ Y++PL+ +
Sbjct: 935 WLAGACMAASSVSVVCSSLLLQLYKKPLHVE 965

BLAST of Bhi10G001408 vs. Swiss-Prot
Match: sp|Q9SH30|HMA5_ARATH (Probable copper-transporting ATPase HMA5 OS=Arabidopsis thaliana OX=3702 GN=HMA5 PE=1 SV=2)

HSP 1 Score: 327.0 bits (837), Expect = 2.4e-88
Identity = 173/327 (52.91%), Postives = 229/327 (70.03%), Query Frame = 0

Query: 2   VKTVVFDKTGTLTVGRPQVVSVVLFSTVPMQELCDAAIAIESNSKHPIAKSVVEHANKMR 61
           V  +VFDKTGTLT+G+P VV   L   + ++E  +   A E NS+HP+AK++VE+A K R
Sbjct: 655 VNCIVFDKTGTLTMGKPVVVKTKLLKNMVLREFYELVAATEVNSEHPLAKAIVEYAKKFR 714

Query: 62  RKFGARSECRKRIQNFEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVPVSPQVDRYAIENE 121
                 +       +F    G GV   +  + ++VGN+ LM  H V +    +    ++E
Sbjct: 715 D--DEENPAWPEACDFVSITGKGVKATVKGREIMVGNKNLMNDHKVIIPDDAEELLADSE 774

Query: 122 RLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILVTGDNWATAFAVARG 181
            +AQT +LV+IN ++ G   V D  KP  +  IS L+S+ I +I+VTGDNW TA ++AR 
Sbjct: 775 DMAQTGILVSINSELIGVLSVSDPLKPSAREAISILKSMNIKSIMVTGDNWGTANSIARE 834

Query: 182 VGINEVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 241
           VGI+ V AE  P  KA K+K  ++ G++VAMVGDG+NDS AL AADVG+AIGAGT+IAIE
Sbjct: 835 VGIDSVIAEAKPEQKAEKVKELQAAGHVVAMVGDGINDSPALVAADVGMAIGAGTDIAIE 894

Query: 242 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP 301
           AADIVLM+SNLEDV+TAIDLSR+T  RI LNY+WALGYN++G+PIAAG+L+P    RLPP
Sbjct: 895 AADIVLMKSNLEDVITAIDLSRKTFSRIRLNYVWALGYNLMGIPIAAGVLFPGTRFRLPP 954

Query: 302 WLAGACMAASSLSVVCSSLLLKCYRRP 329
           W+AGA MAASS+SVVC SLLLK Y+RP
Sbjct: 955 WIAGAAMAASSVSVVCCSLLLKNYKRP 979

BLAST of Bhi10G001408 vs. Swiss-Prot
Match: sp|A3AWA4|HMA5_ORYSJ (Copper-transporting ATPase HMA5 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA5 PE=2 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 2.5e-85
Identity = 168/327 (51.38%), Postives = 224/327 (68.50%), Query Frame = 0

Query: 2   VKTVVFDKTGTLTVGRPQVVSVVLFSTVPMQELCDAAIAIESNSKHPIAKSVVEHANKMR 61
           V  +VFDKTGTLT+G+P VV+  L   + ++E      A E NS+HP+ K+VVEHA K  
Sbjct: 675 VDCIVFDKTGTLTIGKPVVVNTRLLKNMVLREFYAYVAAAEVNSEHPLGKAVVEHAKKFH 734

Query: 62  RKFGARSECRKRIQNFEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVPVSPQVDRYAIENE 121
            +    S      ++F    G GV  +I  + V+VGN+  M    + +  +      E E
Sbjct: 735 SE---ESHVWTEARDFISVTGHGVKAKISGRAVMVGNKSFMLTSGIDIPVEALEILTEEE 794

Query: 122 RLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILVTGDNWATAFAVARG 181
             AQT ++VA++ ++ G   V D  KP  + VIS+L+S+ + +I+VTGDNW TA A+++ 
Sbjct: 795 EKAQTAIIVAMDQEVVGIISVSDPIKPNAREVISYLKSMKVESIMVTGDNWGTANAISKE 854

Query: 182 VGINEVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 241
           VGI    AE  P  KA K+K  +S G  VAMVGDG+NDS AL +ADVG+AIGAGT++AIE
Sbjct: 855 VGIENTVAEAKPEQKAEKVKELQSAGRTVAMVGDGINDSPALVSADVGLAIGAGTDVAIE 914

Query: 242 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP 301
           AADIVLM+SNLEDV+TAIDLSR+T +RI +NY+WALGYNIIG+PIAAG+L+P    RLPP
Sbjct: 915 AADIVLMKSNLEDVITAIDLSRKTFFRIRMNYVWALGYNIIGIPIAAGVLFPSTRFRLPP 974

Query: 302 WLAGACMAASSLSVVCSSLLLKCYRRP 329
           W+AGA MAASS+SVVC SLLL+ Y+ P
Sbjct: 975 WVAGAAMAASSVSVVCWSLLLRYYKSP 998

BLAST of Bhi10G001408 vs. Swiss-Prot
Match: sp|Q9S7J8|HMA7_ARATH (Copper-transporting ATPase RAN1 OS=Arabidopsis thaliana OX=3702 GN=RAN1 PE=1 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 2.2e-81
Identity = 166/340 (48.82%), Postives = 231/340 (67.94%), Query Frame = 0

Query: 2   VKTVVFDKTGTLTVGRPQVVSVVLFSTVPMQELCDAAIAIESNSKHPIAKSVVEHANKMR 61
           VK V+FDKTGTLT G+  V +  +FS +   E      + E++S+HP+AK++V +A    
Sbjct: 652 VKYVIFDKTGTLTQGKATVTTTKVFSEMDRGEFLTLVASAEASSEHPLAKAIVAYARHF- 711

Query: 62  RKFGARSE----CRKRIQN---------FEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVP 121
             F   +E      K +QN         F   PG G+   ++ K +LVGNR+LM  +++ 
Sbjct: 712 HFFDESTEDGETNNKDLQNSGWLLDTSDFSALPGKGIQCLVNEKMILVGNRKLMSENAIN 771

Query: 122 VSPQVDRYAIENERLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILVT 181
           +   V+++  + E   +T V+VA NGK+ G  G+ D  K     V+  L  +G+  I+VT
Sbjct: 772 IPDHVEKFVEDLEESGKTGVIVAYNGKLVGVMGIADPLKREAALVVEGLLRMGVRPIMVT 831

Query: 182 GDNWATAFAVARGVGINEVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAADV 241
           GDNW TA AVA+ VGI +V AE+ P GKA+ I+S +  G+ VAMVGDG+NDS ALAAADV
Sbjct: 832 GDNWRTARAVAKEVGIEDVRAEVMPAGKADVIRSLQKDGSTVAMVGDGINDSPALAAADV 891

Query: 242 GIAIGAGTNIAIEAADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAA 301
           G+AIGAGT++AIEAAD VLMR+NLEDV+TAIDLSR+T+ RI LNY++A+ YN++ +PIAA
Sbjct: 892 GMAIGAGTDVAIEAADYVLMRNNLEDVITAIDLSRKTLTRIRLNYVFAMAYNVVSIPIAA 951

Query: 302 GILYPFFGIRLPPWLAGACMAASSLSVVCSSLLLKCYRRP 329
           G+ +P   ++LPPW AGACMA SS+SVVCSSLLL+ Y++P
Sbjct: 952 GVFFPVLRVQLPPWAAGACMALSSVSVVCSSLLLRRYKKP 990

BLAST of Bhi10G001408 vs. Swiss-Prot
Match: sp|A0A0P0X004|HMA9_ORYSJ (Cation-transporting ATPase HMA5 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA9 PE=2 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 5.4e-80
Identity = 163/341 (47.80%), Postives = 233/341 (68.33%), Query Frame = 0

Query: 2   VKTVVFDKTGTLTVGRPQVVSVVLFSTVPMQELCDAAIAIESNSKHPIAKSVVEHA---- 61
           V  V+FDKTGTLT G+  V +  +FS + + +      + E++S+HP+AK++VE+A    
Sbjct: 652 VNYVIFDKTGTLTQGKAVVTTAKVFSGMDLGDFLTLVASAEASSEHPLAKAIVEYAFHFH 711

Query: 62  --NKM--------RRKFGARSECRKRIQNFEVFPGGGVGGRIDRKTVLVGNRRLMRAHSV 121
              K+        +RK    S+   ++++F   PG GV   I+ K VLVGNR L+  + V
Sbjct: 712 FFGKLPTSKDGIEQRKEDRLSQLLLQVEDFSALPGKGVQCLINGKRVLVGNRTLVTENGV 771

Query: 122 PVSPQVDRYAIENERLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILV 181
            V P+ + + ++ E  A+T +LV+ +    G  G+ D  K     V+  L+ +G+  +++
Sbjct: 772 NVPPEAENFLVDLELNAKTGILVSYDDDFVGLMGITDPLKREAAVVVEGLKKMGVHPVML 831

Query: 182 TGDNWATAFAVARGVGINEVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAAD 241
           TGDNW TA AVA+ VGI +V AE+ P GKA+ ++S +  G+IVAMVGDG+NDS ALAAAD
Sbjct: 832 TGDNWRTAKAVAKEVGIEDVRAEVMPAGKADVVRSLQKDGSIVAMVGDGINDSPALAAAD 891

Query: 242 VGIAIGAGTNIAIEAADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIA 301
           VG+AIG GT+IAIEAAD VL+R+NLEDV+TAIDLSR+T  RI  NY +A+ YN++ +P+A
Sbjct: 892 VGMAIGGGTDIAIEAADYVLVRNNLEDVITAIDLSRKTFSRIRWNYFFAMAYNVVAIPVA 951

Query: 302 AGILYPFFGIRLPPWLAGACMAASSLSVVCSSLLLKCYRRP 329
           AG L+PF  +++PPWLAGACMA SS+SVVCSSLLL+ YR+P
Sbjct: 952 AGALFPFTRLQMPPWLAGACMAFSSVSVVCSSLLLRRYRKP 992

BLAST of Bhi10G001408 vs. TrEMBL
Match: tr|A0A0A0LLU8|A0A0A0LLU8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G279210 PE=3 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 5.6e-167
Identity = 303/332 (91.27%), Postives = 318/332 (95.78%), Query Frame = 0

Query: 2   VKTVVFDKTGTLTVGRPQVVSVVLFSTVPMQELCDAAIAIESNSKHPIAKSVVEHANKMR 61
           VKTVVFDKTGTLTVGRP+VVSVVLFST PMQE+CDAAIAIESNS HP AK VVEHA KMR
Sbjct: 585 VKTVVFDKTGTLTVGRPEVVSVVLFSTFPMQEVCDAAIAIESNSDHPFAKPVVEHAKKMR 644

Query: 62  RKFGARSECRKRIQNFEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVPVSPQVDRYAIENE 121
           RKFGARSEC KR+QNFEVFPGGGVGG+IDRKTVLVGN+RLMR H+V ++PQVDRY IENE
Sbjct: 645 RKFGARSECCKRVQNFEVFPGGGVGGKIDRKTVLVGNKRLMRVHNVDITPQVDRYTIENE 704

Query: 122 RLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILVTGDNWATAFAVARG 181
           RLAQTCVLVAINGKIAGGFGV+D PKPGTKA+ISFLRSIGISTI++TGDN ATAFAVARG
Sbjct: 705 RLAQTCVLVAINGKIAGGFGVLDTPKPGTKAIISFLRSIGISTIMITGDNLATAFAVARG 764

Query: 182 VGINEVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 241
           VGIN+VFAEMDPI KAN+IKS K+ GNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE
Sbjct: 765 VGINKVFAEMDPIEKANQIKSLKTRGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 824

Query: 242 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP 301
           AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP
Sbjct: 825 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP 884

Query: 302 WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS 334
           WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS
Sbjct: 885 WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS 916

BLAST of Bhi10G001408 vs. TrEMBL
Match: tr|A0A1S4E2G3|A0A1S4E2G3_CUCME (probable copper-transporting ATPase HMA5 OS=Cucumis melo OX=3656 GN=LOC103497370 PE=3 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 7.4e-167
Identity = 302/332 (90.96%), Postives = 320/332 (96.39%), Query Frame = 0

Query: 2   VKTVVFDKTGTLTVGRPQVVSVVLFSTVPMQELCDAAIAIESNSKHPIAKSVVEHANKMR 61
           VKTVVFDKTGTLTVGRP+VVSVVLFST PM E+CDAAIAIESNS+HP AK +VEHA KMR
Sbjct: 560 VKTVVFDKTGTLTVGRPEVVSVVLFSTFPMLEVCDAAIAIESNSEHPFAKPIVEHAKKMR 619

Query: 62  RKFGARSECRKRIQNFEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVPVSPQVDRYAIENE 121
           +KFGARSEC KR+QNFEVFPGGGVGG+IDRKTVLVGN+RLMR H+V ++PQVDRY IENE
Sbjct: 620 KKFGARSECSKRVQNFEVFPGGGVGGKIDRKTVLVGNKRLMRVHNVDITPQVDRYTIENE 679

Query: 122 RLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILVTGDNWATAFAVARG 181
           RLAQTCVLVAI+GKIAGGFGV+DAPKPGTKAVISFLRSIGISTI++TGDN ATAFAVARG
Sbjct: 680 RLAQTCVLVAIDGKIAGGFGVLDAPKPGTKAVISFLRSIGISTIMITGDNLATAFAVARG 739

Query: 182 VGINEVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 241
           VGIN+VFAEMDPI KAN+I+S KSTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE
Sbjct: 740 VGINKVFAEMDPIEKANQIRSLKSTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 799

Query: 242 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP 301
           AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP
Sbjct: 800 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP 859

Query: 302 WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS 334
           WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS
Sbjct: 860 WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS 891

BLAST of Bhi10G001408 vs. TrEMBL
Match: tr|A0A2G5DYB6|A0A2G5DYB6_AQUCA (Uncharacterized protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_01400830v1 PE=3 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 3.2e-122
Identity = 220/333 (66.07%), Postives = 280/333 (84.08%), Query Frame = 0

Query: 2   VKTVVFDKTGTLTVGRPQVVSVVLFSTVPMQELCDAAIAIESNSKHPIAKSVVEHANKMR 61
           VKTVVFDKTGTLTVG+P VVS +LFS VP+Q+ CD A   E+NS+HPI K+VVE+  K+ 
Sbjct: 649 VKTVVFDKTGTLTVGKPVVVSTMLFSDVPIQDFCDMAAIAEANSEHPIGKAVVEYTKKLH 708

Query: 62  RKFGARSECRKRIQNFEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVPVSPQVDRYAIENE 121
           +++G+ +E    +++FEV+PG GVGG ++ K VLVGN+RLM+ H++PV+P V+ Y  E+E
Sbjct: 709 QQYGSNTEHMTEVKDFEVYPGSGVGGNVNGKRVLVGNKRLMKTHNIPVNPDVEDYMSESE 768

Query: 122 RLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILVTGDNWATAFAVARG 181
           +LA+TCVLVA+NG ++G F V D  KP  + VISFLR++GI++I+VTGDNWATA A+A+ 
Sbjct: 769 QLARTCVLVAVNGIVSGAFAVTDPVKPEAERVISFLRTMGITSIMVTGDNWATATAIAKE 828

Query: 182 VGINEVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 241
           VGI  VFAE DP GKANKIK  +  G+ VAMVGDG+NDS AL AADVG+AIGAGT++AIE
Sbjct: 829 VGIQTVFAETDPQGKANKIKDLQMEGDAVAMVGDGINDSPALVAADVGMAIGAGTDVAIE 888

Query: 242 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP 301
           AAD+VL++SNLEDVVTAIDLSR+T+ RI LNY+WALGYN++GMPIAAGIL+PF GIRLPP
Sbjct: 889 AADVVLIKSNLEDVVTAIDLSRKTLSRIRLNYVWALGYNVLGMPIAAGILFPFTGIRLPP 948

Query: 302 WLAGACMAASSLSVVCSSLLLKCYRRPLNFQSA 335
           WLAGACMAASSLSVVCSSLLL+ Y++PL+FQ +
Sbjct: 949 WLAGACMAASSLSVVCSSLLLQSYKKPLHFQDS 981

BLAST of Bhi10G001408 vs. TrEMBL
Match: tr|A0A1R3HDW9|A0A1R3HDW9_COCAP (Cation-transporting P-type ATPase OS=Corchorus capsularis OX=210143 GN=CCACVL1_19922 PE=3 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 2.7e-121
Identity = 219/328 (66.77%), Postives = 278/328 (84.76%), Query Frame = 0

Query: 2   VKTVVFDKTGTLTVGRPQVVSVVLFSTVPMQELCDAAIAIESNSKHPIAKSVVEHANKMR 61
           VK +VFDKTGTLTVG+P+VVSV+LFS++ M+E CD AIA E+NS+HPIAK+ +EHA K+R
Sbjct: 584 VKAIVFDKTGTLTVGKPEVVSVMLFSSMSMEEFCDVAIAAEANSEHPIAKAFLEHARKLR 643

Query: 62  RKFGARSECRKRIQNFEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVPVSPQVDRYAIENE 121
           +K  + ++     ++FEV PG GV G++  K VLVGN+RLM++++V V P ++ Y  ENE
Sbjct: 644 QKIESNNQHLTEARDFEVHPGTGVSGKVGDKMVLVGNKRLMQSYNVTVGPDIESYISENE 703

Query: 122 RLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILVTGDNWATAFAVARG 181
           +LA+TCVLVAI+G++AG F V D  KP  + VIS+LRS+GIS+I+VTGDNWATA A+A+ 
Sbjct: 704 QLARTCVLVAIDGEVAGAFAVTDPVKPEAQHVISYLRSMGISSIMVTGDNWATAAAIAKE 763

Query: 182 VGINEVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 241
           VGI  VFAE DP+GKA++IK F+  G  VAMVGDG+NDS AL AADVG+AIGAGT++AIE
Sbjct: 764 VGIEHVFAETDPVGKADRIKEFQMKGLTVAMVGDGINDSPALVAADVGMAIGAGTDVAIE 823

Query: 242 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP 301
           AAD+VL++SNLEDVVTAIDLSR+T+ RIWLNY+WALGYNI+G+P+AAG+LYPF GIRLPP
Sbjct: 824 AADVVLIKSNLEDVVTAIDLSRKTIARIWLNYVWALGYNILGVPVAAGLLYPFTGIRLPP 883

Query: 302 WLAGACMAASSLSVVCSSLLLKCYRRPL 330
           WLAGACMAASSLSVVCSSLLL+ YR+PL
Sbjct: 884 WLAGACMAASSLSVVCSSLLLQSYRKPL 911

BLAST of Bhi10G001408 vs. TrEMBL
Match: tr|A0A067JRB6|A0A067JRB6_JATCU (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_25905 PE=3 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 3.6e-121
Identity = 225/333 (67.57%), Postives = 277/333 (83.18%), Query Frame = 0

Query: 2   VKTVVFDKTGTLTVGRPQVVSVVLFSTVPMQELCDAAIAIESNSKHPIAKSVVEHANKMR 61
           V  VVFDKTGTLT+G+P VVS V+FS+  M+E CD A + E NS+HPIAK+VVEHA ++R
Sbjct: 632 VNAVVFDKTGTLTIGKPVVVSAVVFSSFSMEEFCDMATSAEVNSEHPIAKAVVEHAKRLR 691

Query: 62  RKFGARSECRKRIQNFEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVPVSPQVDRYAIENE 121
           +  GA+SE    +++FEV  G GV G++  + VLVGNRRLM+A +V V P+V+ Y  ENE
Sbjct: 692 QNIGAKSEHITEVKDFEVHTGAGVTGKVGDRMVLVGNRRLMQACNVMVGPEVENYIAENE 751

Query: 122 RLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILVTGDNWATAFAVARG 181
           +LA+TCVLV+I+GKIAG F V D  KP  + VISFLRS+GIS+++VTGDNWATA A+A+ 
Sbjct: 752 QLARTCVLVSIDGKIAGAFAVTDPVKPEAEHVISFLRSMGISSVMVTGDNWATAAAIAKE 811

Query: 182 VGINEVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 241
           VGI EVFAE DP+GKA++IK  +  G IVAMVGDG+NDS AL AADVG+AIGAGT++AIE
Sbjct: 812 VGIKEVFAETDPLGKADRIKDLQGKGMIVAMVGDGINDSPALVAADVGMAIGAGTDVAIE 871

Query: 242 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP 301
           AADIVL++SNLEDVVTAIDLSR+T++RI LNY+WALGYNI+GMPIAAGILYPF GIRLPP
Sbjct: 872 AADIVLIKSNLEDVVTAIDLSRKTIFRIRLNYVWALGYNILGMPIAAGILYPFTGIRLPP 931

Query: 302 WLAGACMAASSLSVVCSSLLLKCYRRPLNFQSA 335
           WLAGACMAASSLSVVCSSLLL+ Y++PL   S+
Sbjct: 932 WLAGACMAASSLSVVCSSLLLQSYKKPLRVSSS 964

BLAST of Bhi10G001408 vs. NCBI nr
Match: XP_022947845.1 (copper-transporting ATPase HMA4-like [Cucurbita moschata])

HSP 1 Score: 600.9 bits (1548), Expect = 2.6e-168
Identity = 305/332 (91.87%), Postives = 320/332 (96.39%), Query Frame = 0

Query: 2   VKTVVFDKTGTLTVGRPQVVSVVLFSTVPMQELCDAAIAIESNSKHPIAKSVVEHANKMR 61
           VK VVFDKTGTLTVGRP+VVSVVLFST PMQELCD AIAIESNSKHP+AK VV+HA KMR
Sbjct: 574 VKMVVFDKTGTLTVGRPEVVSVVLFSTFPMQELCDVAIAIESNSKHPLAKCVVDHAMKMR 633

Query: 62  RKFGARSECRKRIQNFEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVPVSPQVDRYAIENE 121
           +KFGAR+ECRKRIQNFEVFPGGGVGG+IDRK VLVGNRRLMRAH++ V PQVDRY IENE
Sbjct: 634 KKFGARTECRKRIQNFEVFPGGGVGGKIDRKPVLVGNRRLMRAHNIVVGPQVDRYVIENE 693

Query: 122 RLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILVTGDNWATAFAVARG 181
           RLAQTC+LVAING++AGGFGVIDAPK GTKAVIS+LRSIGIS+I+VTGDNWATAFAVARG
Sbjct: 694 RLAQTCILVAINGRVAGGFGVIDAPKSGTKAVISYLRSIGISSIMVTGDNWATAFAVARG 753

Query: 182 VGINEVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 241
           VGINEVFAEMDPIGKANKIKS K+TGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE
Sbjct: 754 VGINEVFAEMDPIGKANKIKSLKTTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 813

Query: 242 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP 301
           AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPF GIRLPP
Sbjct: 814 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFTGIRLPP 873

Query: 302 WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS 334
           WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS
Sbjct: 874 WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS 905

BLAST of Bhi10G001408 vs. NCBI nr
Match: XP_023007109.1 (copper-transporting ATPase HMA4-like, partial [Cucurbita maxima])

HSP 1 Score: 600.9 bits (1548), Expect = 2.6e-168
Identity = 305/332 (91.87%), Postives = 320/332 (96.39%), Query Frame = 0

Query: 2   VKTVVFDKTGTLTVGRPQVVSVVLFSTVPMQELCDAAIAIESNSKHPIAKSVVEHANKMR 61
           VK VVFDKTGTLTVGRP+VVSVVLFST PMQELCD AIAIESNSKHP+AK VV+HA KMR
Sbjct: 557 VKMVVFDKTGTLTVGRPEVVSVVLFSTFPMQELCDVAIAIESNSKHPLAKCVVDHAMKMR 616

Query: 62  RKFGARSECRKRIQNFEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVPVSPQVDRYAIENE 121
           +KFGAR+ECRKRIQNFEVFPGGGVGG+IDRK VLVGNRRLMRAH++ V PQVDRY IENE
Sbjct: 617 KKFGARTECRKRIQNFEVFPGGGVGGKIDRKPVLVGNRRLMRAHNIVVGPQVDRYVIENE 676

Query: 122 RLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILVTGDNWATAFAVARG 181
           RLAQTC+LVAING++AGGFGVIDAPK GTKAVIS+LRSIGIS+I+VTGDNWATAFAVARG
Sbjct: 677 RLAQTCILVAINGRVAGGFGVIDAPKSGTKAVISYLRSIGISSIMVTGDNWATAFAVARG 736

Query: 182 VGINEVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 241
           VGINEVFAEMDPIGKANKIKS K+TGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE
Sbjct: 737 VGINEVFAEMDPIGKANKIKSLKTTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 796

Query: 242 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP 301
           AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPF GIRLPP
Sbjct: 797 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFTGIRLPP 856

Query: 302 WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS 334
           WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS
Sbjct: 857 WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS 888

BLAST of Bhi10G001408 vs. NCBI nr
Match: XP_023533115.1 (copper-transporting ATPase HMA4-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 600.9 bits (1548), Expect = 2.6e-168
Identity = 305/332 (91.87%), Postives = 320/332 (96.39%), Query Frame = 0

Query: 2   VKTVVFDKTGTLTVGRPQVVSVVLFSTVPMQELCDAAIAIESNSKHPIAKSVVEHANKMR 61
           VK VVFDKTGTLTVGRP+VVSVVLFST PMQELCD AIAIESNSKHP+AK VV+HA KMR
Sbjct: 551 VKMVVFDKTGTLTVGRPEVVSVVLFSTFPMQELCDVAIAIESNSKHPLAKCVVDHAMKMR 610

Query: 62  RKFGARSECRKRIQNFEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVPVSPQVDRYAIENE 121
           +KFGAR+ECRKRIQNFEVFPGGGVGG+IDRK VLVGNRRLMRAH++ V PQVDRY IENE
Sbjct: 611 KKFGARTECRKRIQNFEVFPGGGVGGKIDRKPVLVGNRRLMRAHNIVVGPQVDRYVIENE 670

Query: 122 RLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILVTGDNWATAFAVARG 181
           RLAQTC+LVAING++AGGFGVIDAPK GTKAVIS+LRSIGIS+I+VTGDNWATAFAVARG
Sbjct: 671 RLAQTCILVAINGRVAGGFGVIDAPKSGTKAVISYLRSIGISSIMVTGDNWATAFAVARG 730

Query: 182 VGINEVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 241
           VGINEVFAEMDPIGKANKIKS K+TGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE
Sbjct: 731 VGINEVFAEMDPIGKANKIKSLKTTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 790

Query: 242 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP 301
           AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPF GIRLPP
Sbjct: 791 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFTGIRLPP 850

Query: 302 WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS 334
           WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS
Sbjct: 851 WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS 882

BLAST of Bhi10G001408 vs. NCBI nr
Match: XP_011649326.1 (PREDICTED: probable copper-transporting ATPase HMA5 isoform X1 [Cucumis sativus] >XP_011649328.1 PREDICTED: probable copper-transporting ATPase HMA5 isoform X1 [Cucumis sativus] >KGN61984.1 hypothetical protein Csa_2G279210 [Cucumis sativus])

HSP 1 Score: 595.9 bits (1535), Expect = 8.5e-167
Identity = 303/332 (91.27%), Postives = 318/332 (95.78%), Query Frame = 0

Query: 2   VKTVVFDKTGTLTVGRPQVVSVVLFSTVPMQELCDAAIAIESNSKHPIAKSVVEHANKMR 61
           VKTVVFDKTGTLTVGRP+VVSVVLFST PMQE+CDAAIAIESNS HP AK VVEHA KMR
Sbjct: 585 VKTVVFDKTGTLTVGRPEVVSVVLFSTFPMQEVCDAAIAIESNSDHPFAKPVVEHAKKMR 644

Query: 62  RKFGARSECRKRIQNFEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVPVSPQVDRYAIENE 121
           RKFGARSEC KR+QNFEVFPGGGVGG+IDRKTVLVGN+RLMR H+V ++PQVDRY IENE
Sbjct: 645 RKFGARSECCKRVQNFEVFPGGGVGGKIDRKTVLVGNKRLMRVHNVDITPQVDRYTIENE 704

Query: 122 RLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILVTGDNWATAFAVARG 181
           RLAQTCVLVAINGKIAGGFGV+D PKPGTKA+ISFLRSIGISTI++TGDN ATAFAVARG
Sbjct: 705 RLAQTCVLVAINGKIAGGFGVLDTPKPGTKAIISFLRSIGISTIMITGDNLATAFAVARG 764

Query: 182 VGINEVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 241
           VGIN+VFAEMDPI KAN+IKS K+ GNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE
Sbjct: 765 VGINKVFAEMDPIEKANQIKSLKTRGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 824

Query: 242 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP 301
           AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP
Sbjct: 825 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP 884

Query: 302 WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS 334
           WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS
Sbjct: 885 WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS 916

BLAST of Bhi10G001408 vs. NCBI nr
Match: XP_004149709.2 (PREDICTED: probable copper-transporting ATPase HMA5 isoform X2 [Cucumis sativus])

HSP 1 Score: 595.9 bits (1535), Expect = 8.5e-167
Identity = 303/332 (91.27%), Postives = 318/332 (95.78%), Query Frame = 0

Query: 2   VKTVVFDKTGTLTVGRPQVVSVVLFSTVPMQELCDAAIAIESNSKHPIAKSVVEHANKMR 61
           VKTVVFDKTGTLTVGRP+VVSVVLFST PMQE+CDAAIAIESNS HP AK VVEHA KMR
Sbjct: 560 VKTVVFDKTGTLTVGRPEVVSVVLFSTFPMQEVCDAAIAIESNSDHPFAKPVVEHAKKMR 619

Query: 62  RKFGARSECRKRIQNFEVFPGGGVGGRIDRKTVLVGNRRLMRAHSVPVSPQVDRYAIENE 121
           RKFGARSEC KR+QNFEVFPGGGVGG+IDRKTVLVGN+RLMR H+V ++PQVDRY IENE
Sbjct: 620 RKFGARSECCKRVQNFEVFPGGGVGGKIDRKTVLVGNKRLMRVHNVDITPQVDRYTIENE 679

Query: 122 RLAQTCVLVAINGKIAGGFGVIDAPKPGTKAVISFLRSIGISTILVTGDNWATAFAVARG 181
           RLAQTCVLVAINGKIAGGFGV+D PKPGTKA+ISFLRSIGISTI++TGDN ATAFAVARG
Sbjct: 680 RLAQTCVLVAINGKIAGGFGVLDTPKPGTKAIISFLRSIGISTIMITGDNLATAFAVARG 739

Query: 182 VGINEVFAEMDPIGKANKIKSFKSTGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 241
           VGIN+VFAEMDPI KAN+IKS K+ GNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE
Sbjct: 740 VGINKVFAEMDPIEKANQIKSLKTRGNIVAMVGDGVNDSHALAAADVGIAIGAGTNIAIE 799

Query: 242 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP 301
           AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP
Sbjct: 800 AADIVLMRSNLEDVVTAIDLSRQTVYRIWLNYIWALGYNIIGMPIAAGILYPFFGIRLPP 859

Query: 302 WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS 334
           WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS
Sbjct: 860 WLAGACMAASSLSVVCSSLLLKCYRRPLNFQS 891

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G63440.11.3e-8952.91heavy metal atpase 5[more]
AT5G44790.11.2e-8248.82copper-exporting ATPase / responsive-to-antagonist 1 / copper-transporting ATPas... [more]
AT4G33520.24.5e-4536.72P-type ATP-ase 1[more]
AT5G21930.12.7e-4235.67P-type ATPase of Arabidopsis 2[more]
AT2G19110.14.4e-3230.09heavy metal atpase 4[more]
Match NameE-valueIdentityDescription
sp|Q6H7M3|HMA4_ORYSJ4.8e-11361.33Copper-transporting ATPase HMA4 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA4... [more]
sp|Q9SH30|HMA5_ARATH2.4e-8852.91Probable copper-transporting ATPase HMA5 OS=Arabidopsis thaliana OX=3702 GN=HMA5... [more]
sp|A3AWA4|HMA5_ORYSJ2.5e-8551.38Copper-transporting ATPase HMA5 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA5... [more]
sp|Q9S7J8|HMA7_ARATH2.2e-8148.82Copper-transporting ATPase RAN1 OS=Arabidopsis thaliana OX=3702 GN=RAN1 PE=1 SV=... [more]
sp|A0A0P0X004|HMA9_ORYSJ5.4e-8047.80Cation-transporting ATPase HMA5 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA9... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0LLU8|A0A0A0LLU8_CUCSA5.6e-16791.27Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G279210 PE=3 SV=1[more]
tr|A0A1S4E2G3|A0A1S4E2G3_CUCME7.4e-16790.96probable copper-transporting ATPase HMA5 OS=Cucumis melo OX=3656 GN=LOC103497370... [more]
tr|A0A2G5DYB6|A0A2G5DYB6_AQUCA3.2e-12266.07Uncharacterized protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_01400830v1 PE=3... [more]
tr|A0A1R3HDW9|A0A1R3HDW9_COCAP2.7e-12166.77Cation-transporting P-type ATPase OS=Corchorus capsularis OX=210143 GN=CCACVL1_1... [more]
tr|A0A067JRB6|A0A067JRB6_JATCU3.6e-12167.57Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_25905 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022947845.12.6e-16891.87copper-transporting ATPase HMA4-like [Cucurbita moschata][more]
XP_023007109.12.6e-16891.87copper-transporting ATPase HMA4-like, partial [Cucurbita maxima][more]
XP_023533115.12.6e-16891.87copper-transporting ATPase HMA4-like, partial [Cucurbita pepo subsp. pepo][more]
XP_011649326.18.5e-16791.27PREDICTED: probable copper-transporting ATPase HMA5 isoform X1 [Cucumis sativus]... [more]
XP_004149709.28.5e-16791.27PREDICTED: probable copper-transporting ATPase HMA5 isoform X2 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0000166nucleotide binding
Vocabulary: Cellular Component
TermDefinition
GO:0016021integral component of membrane
Vocabulary: INTERPRO
TermDefinition
IPR036412HAD-like_sf
IPR018303ATPase_P-typ_P_site
IPR023299ATPase_P-typ_cyto_dom_N
IPR023214HAD_sf
IPR001757P_typ_ATPase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098655 cation transmembrane transport
biological_process GO:0030001 metal ion transport
biological_process GO:0060003 copper ion export
biological_process GO:0008152 metabolic process
biological_process GO:0006812 cation transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0019829 cation-transporting ATPase activity
molecular_function GO:0005507 copper ion binding
molecular_function GO:0004008 copper-exporting ATPase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0000166 nucleotide binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi10M001408Bhi10M001408mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001757P-type ATPasePRINTSPR00120HATPASEcoord: 213..229
score: 63.53
coord: 244..269
score: 35.58
IPR001757P-type ATPaseTIGRFAMTIGR01494TIGR01494coord: 2..290
e-value: 1.0E-48
score: 163.9
NoneNo IPR availablePRINTSPR00119CATATPASEcoord: 6..20
score: 56.53
coord: 213..232
score: 63.32
coord: 236..248
score: 46.37
coord: 160..170
score: 50.15
coord: 138..149
score: 31.87
NoneNo IPR availablePFAMPF00702Hydrolasecoord: 2..227
e-value: 2.9E-41
score: 142.0
NoneNo IPR availableSFLDSFLDG00002C1.7:_P-type_atpase_likecoord: 2..265
e-value: 0.0
score: 162.1
NoneNo IPR availableSFLDSFLDF00027p-type_atpasecoord: 2..265
e-value: 0.0
score: 162.1
NoneNo IPR availablePANTHERPTHR24093:SF417SUBFAMILY NOT NAMEDcoord: 2..326
NoneNo IPR availablePANTHERPTHR24093CATION TRANSPORTING ATPASEcoord: 2..326
IPR023214HAD superfamilyGENE3DG3DSA:3.40.50.1000coord: 146..263
e-value: 6.7E-83
score: 280.2
coord: 2..16
e-value: 6.7E-83
score: 280.2
IPR023299P-type ATPase, cytoplasmic domain NGENE3DG3DSA:3.40.1110.10coord: 17..145
e-value: 6.7E-83
score: 280.2
IPR018303P-type ATPase, phosphorylation sitePROSITEPS00154ATPASE_E1_E2coord: 8..14
IPR036412HAD-like superfamilySUPERFAMILYSSF56784HAD-likecoord: 70..319
coord: 4..24