Bhi10G001301 (gene) Wax gourd

NameBhi10G001301
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionCytochrome P450, putative
Locationchr10 : 40932488 .. 40933432 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGACCACCTGGTTCATCATCCTCATCTCTCTCTGCATTTGCTATCTTTTCAGTTCCATTTTTATCAAATTCCGAAGCTCCCCAAAGCTTCCACCAGGCCCTCCTTCAATCCCCTTCATTTCCACTTTCCTATGGCTCCGTACATCGCCTCTGCAAATGGAGTCTTACCTCCGCACCGCCGTCGCCAAATACGGCCCCATTATCACCCTCCGCATCGGCTCTCGCCCTTCCATCTTCATCGTCGACCGCACCATTGCCCACAAAGCTCTGCTTCAACACGGCACTCTCTTTGCTGACCGCCCACCACCTCCTCCCTTGACCAAAATCCTCTCAAGCAACCAACACAGCATCAACTCCGCCGCTTACGGCCCACTCTGGCGCCTCCTCCGCCGCAATCTCACTTCCCAAATCCTCCATCCTTCCCGTATGAGATTGTACGGTCGTGCTCGCAAGTGGGTTTTGGATATTCTTCTTAATCGTCTTCTCTCTCACTCTGAATCGGGAAGTCCCGTCTATGTTGTGGATCACTTTCAGTACGCCATGTTCTGTTTGTTGGTGTTAATGTGCTTCGGGGATAAACTTGACGAATCCCAAATCAAAGAAGTCGAGAATGTGCAGCGTACAATGCTACTAAATTTTATGCGTTTTGGTCTCCTCAATTTATATCCGAAATTGACCAAGATTTTTCTTCCAAAGCGCTGGGAGGAGTTTCTCCAACTAAGAAAGAACCAAGAGAGAGTCATAATCCGTCTAACCGAAGCAAGAAGGGAGGCCAAGAAAAACAGAGCAAACAAAGTAAACAAAGGAAACAGAGAAGGTGAAGAAGAAGAAAAAGAAGATGATGAAGAATTCGTGTTGTCATATGTGGATACACTCCTTGAATTACAACTCCCTCACGAGGATAATAGAAAGTTAACCGACGGTGAAATTGTCACATTATGA

mRNA sequence

ATGGAGACCACCTGGTTCATCATCCTCATCTCTCTCTGCATTTGCTATCTTTTCAGTTCCATTTTTATCAAATTCCGAAGCTCCCCAAAGCTTCCACCAGGCCCTCCTTCAATCCCCTTCATTTCCACTTTCCTATGGCTCCGTACATCGCCTCTGCAAATGGAGTCTTACCTCCGCACCGCCGTCGCCAAATACGGCCCCATTATCACCCTCCGCATCGGCTCTCGCCCTTCCATCTTCATCGTCGACCGCACCATTGCCCACAAAGCTCTGCTTCAACACGGCACTCTCTTTGCTGACCGCCCACCACCTCCTCCCTTGACCAAAATCCTCTCAAGCAACCAACACAGCATCAACTCCGCCGCTTACGGCCCACTCTGGCGCCTCCTCCGCCGCAATCTCACTTCCCAAATCCTCCATCCTTCCCGTATGAGATTGTACGGTCGTGCTCGCAAGTGGGTTTTGGATATTCTTCTTAATCGTCTTCTCTCTCACTCTGAATCGGGAAGTCCCGTCTATGTTGTGGATCACTTTCAGTACGCCATGTTCTGTTTGTTGGTGTTAATGTGCTTCGGGGATAAACTTGACGAATCCCAAATCAAAGAAGTCGAGAATGTGCAGCGTACAATGCTACTAAATTTTATGCGTTTTGGTCTCCTCAATTTATATCCGAAATTGACCAAGATTTTTCTTCCAAAGCGCTGGGAGGAGTTTCTCCAACTAAGAAAGAACCAAGAGAGAGTCATAATCCGTCTAACCGAAGCAAGAAGGGAGGCCAAGAAAAACAGAGCAAACAAAGTAAACAAAGGAAACAGAGAAGGTGAAGAAGAAGAAAAAGAAGATGATGAAGAATTCGTGTTGTCATATGTGGATACACTCCTTGAATTACAACTCCCTCACGAGGATAATAGAAAGTTAACCGACGGTGAAATTGTCACATTATGA

Coding sequence (CDS)

ATGGAGACCACCTGGTTCATCATCCTCATCTCTCTCTGCATTTGCTATCTTTTCAGTTCCATTTTTATCAAATTCCGAAGCTCCCCAAAGCTTCCACCAGGCCCTCCTTCAATCCCCTTCATTTCCACTTTCCTATGGCTCCGTACATCGCCTCTGCAAATGGAGTCTTACCTCCGCACCGCCGTCGCCAAATACGGCCCCATTATCACCCTCCGCATCGGCTCTCGCCCTTCCATCTTCATCGTCGACCGCACCATTGCCCACAAAGCTCTGCTTCAACACGGCACTCTCTTTGCTGACCGCCCACCACCTCCTCCCTTGACCAAAATCCTCTCAAGCAACCAACACAGCATCAACTCCGCCGCTTACGGCCCACTCTGGCGCCTCCTCCGCCGCAATCTCACTTCCCAAATCCTCCATCCTTCCCGTATGAGATTGTACGGTCGTGCTCGCAAGTGGGTTTTGGATATTCTTCTTAATCGTCTTCTCTCTCACTCTGAATCGGGAAGTCCCGTCTATGTTGTGGATCACTTTCAGTACGCCATGTTCTGTTTGTTGGTGTTAATGTGCTTCGGGGATAAACTTGACGAATCCCAAATCAAAGAAGTCGAGAATGTGCAGCGTACAATGCTACTAAATTTTATGCGTTTTGGTCTCCTCAATTTATATCCGAAATTGACCAAGATTTTTCTTCCAAAGCGCTGGGAGGAGTTTCTCCAACTAAGAAAGAACCAAGAGAGAGTCATAATCCGTCTAACCGAAGCAAGAAGGGAGGCCAAGAAAAACAGAGCAAACAAAGTAAACAAAGGAAACAGAGAAGGTGAAGAAGAAGAAAAAGAAGATGATGAAGAATTCGTGTTGTCATATGTGGATACACTCCTTGAATTACAACTCCCTCACGAGGATAATAGAAAGTTAACCGACGGTGAAATTGTCACATTATGA

Protein sequence

METTWFIILISLCICYLFSSIFIKFRSSPKLPPGPPSIPFISTFLWLRTSPLQMESYLRTAVAKYGPIITLRIGSRPSIFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQHSINSAAYGPLWRLLRRNLTSQILHPSRMRLYGRARKWVLDILLNRLLSHSESGSPVYVVDHFQYAMFCLLVLMCFGDKLDESQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRWEEFLQLRKNQERVIIRLTEARREAKKNRANKVNKGNREGEEEEKEDDEEFVLSYVDTLLELQLPHEDNRKLTDGEIVTL
BLAST of Bhi10G001301 vs. TAIR10
Match: AT1G64950.1 (cytochrome P450, family 89, subfamily A, polypeptide 5)

HSP 1 Score: 298.9 bits (764), Expect = 3.7e-81
Identity = 185/312 (59.29%), Postives = 227/312 (72.76%), Query Frame = 0

Query: 5   WFIILISLCICYLFSSIFIKFR--SSPKLPPGPPSIPFISTFLWLRTSPLQMESYLRTAV 64
           W +IL SL +  L + +F + R  SS  LPP P   PFI T  WLR     + +YLR+  
Sbjct: 4   WLLILGSLFLSLLLNLLFFRLRDSSSLPLPPDPNYFPFIGTIQWLRQGLGGLNNYLRSVH 63

Query: 65  AKYGPIITLRIGSRPSIFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQHSINSAA 124
            + GPIITLRI SRPSIF+ DR++AH+AL+ +G +FADRPP  P++KI+SSNQH+I+S  
Sbjct: 64  HRLGPIITLRITSRPSIFVADRSLAHQALVLNGAVFADRPPAAPISKIISSNQHNISSCL 123

Query: 125 YGPLWRLLRRNLTSQILHPSRMRLYGRARKWVLDILLNRLLSHSESGSPVYVVDHFQYAM 184
           YG  WRLLRRNLTS+ILHPSR+R Y  AR+WVL+IL +R    +    P+ VVDH  YAM
Sbjct: 124 YGATWRLLRRNLTSEILHPSRVRSYSHARRWVLEILFDR-FGKNRGEEPIVVVDHLHYAM 183

Query: 185 FCLLVLMCFGDKLDESQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRWEEFLQLR 244
           F LLVLMCFGDKLDE QIK+VE VQR  LL F RF +LNL+PK TK+ L KRWEEF Q+R
Sbjct: 184 FALLVLMCFGDKLDEKQIKQVEYVQRRQLLGFSRFNILNLWPKFTKLILRKRWEEFFQMR 243

Query: 245 KNQERVIIRLTEARREAXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLELQLPHED 304
           + Q  V++ L        XXXXXXXXXXXXXXXXXXXXXXX +V SYVDTLLEL+LP ++
Sbjct: 244 REQHDVLLPL--------XXXXXXXXXXXXXXXXXXXXXXXEYVQSYVDTLLELELP-DE 303

Query: 305 NRKLTDGEIVTL 315
            RKL + EIV+L
Sbjct: 304 KRKLNEDEIVSL 305

BLAST of Bhi10G001301 vs. TAIR10
Match: AT2G12190.1 (Cytochrome P450 superfamily protein)

HSP 1 Score: 295.0 bits (754), Expect = 5.3e-80
Identity = 181/312 (58.01%), Postives = 225/312 (72.12%), Query Frame = 0

Query: 5   WFIILISLCICYLFSSIFIKFR--SSPKLPPGPPSIPFISTFLWLRTSPLQMESYLRTAV 64
           W +IL SL +  L + +  + R  SS  LPP P   PF+ T  WLR     + +YLR+  
Sbjct: 4   WLLILGSLFLSLLLNLLLFRRRDSSSLPLPPDPNFFPFLGTLQWLRQGLGGLNNYLRSVH 63

Query: 65  AKYGPIITLRIGSRPSIFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQHSINSAA 124
            + GPIITLRI SRP+IF+ DR++AH+AL+ +G +FADRPP  P++KI+SSNQH+I+S+ 
Sbjct: 64  HRLGPIITLRITSRPAIFVADRSLAHQALVLNGAVFADRPPAAPISKIISSNQHNISSSL 123

Query: 125 YGPLWRLLRRNLTSQILHPSRMRLYGRARKWVLDILLNRLLSHSESGSPVYVVDHFQYAM 184
           YG  WRLLRRNLTS+ILHPSR+R Y  AR+WVL+IL +R    S    P+ VVDH  YAM
Sbjct: 124 YGATWRLLRRNLTSEILHPSRVRSYSHARRWVLEILFDR-FGKSRGEEPIVVVDHLHYAM 183

Query: 185 FCLLVLMCFGDKLDESQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRWEEFLQLR 244
           F LLVLMCFGDKLDE QIK+VE VQR  LL F RF +LNL+PK TK+ L KRWEEF Q+R
Sbjct: 184 FALLVLMCFGDKLDEKQIKQVEYVQRRQLLGFSRFNILNLWPKFTKLILRKRWEEFFQMR 243

Query: 245 KNQERVIIRLTEARREAXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLELQLPHED 304
           + Q  V++ L         XXXXXXXXXXXXXXXXXXXXX  +V SYVDTLLEL+LP ++
Sbjct: 244 REQHDVLLPL--------IXXXXXXXXXXXXXXXXXXXXXKVYVQSYVDTLLELELP-DE 303

Query: 305 NRKLTDGEIVTL 315
            RKL + EIV+L
Sbjct: 304 KRKLNEDEIVSL 305

BLAST of Bhi10G001301 vs. TAIR10
Match: AT1G64940.1 (cytochrome P450, family 87, subfamily A, polypeptide 6)

HSP 1 Score: 282.3 bits (721), Expect = 3.6e-76
Identity = 175/313 (55.91%), Postives = 220/313 (70.29%), Query Frame = 0

Query: 5   WFIILISLCICYLFSSIFIKFR---SSPKLPPGPPSIPFISTFLWLRTSPLQMESYLRTA 64
           W +IL SL +  L + +  + R   SS  LPP P   PFI T  WLR     +++YLR+ 
Sbjct: 4   WLLILGSLFLSLLVNHLLFRRRDSFSSLPLPPDPNFFPFIGTLKWLRKGLGGLDNYLRSV 63

Query: 65  VAKYGPIITLRIGSRPSIFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQHSINSA 124
               GPIITLRI SRP+IF+ DR++AH+AL+ +G +FADRPP   ++KI+SSNQH+I+S 
Sbjct: 64  HHHLGPIITLRITSRPAIFVTDRSLAHQALVLNGAVFADRPPAESISKIISSNQHNISSC 123

Query: 125 AYGPLWRLLRRNLTSQILHPSRMRLYGRARKWVLDILLNRLLSHSESGSPVYVVDHFQYA 184
            YG  WRLLRRNLTS+ILHPSR+R Y  AR+WVL+IL  R    +    P+ VVDH  YA
Sbjct: 124 LYGATWRLLRRNLTSEILHPSRLRSYSHARRWVLEILFGR-FGKNRGEEPIVVVDHLHYA 183

Query: 185 MFCLLVLMCFGDKLDESQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRWEEFLQL 244
           MF LLVLMCFGDKLDE QIK+VE VQR  LL F RF +L L+PK TK+   KRWEEF Q+
Sbjct: 184 MFALLVLMCFGDKLDEKQIKQVEYVQRRQLLGFSRFNILTLWPKFTKLIYRKRWEEFFQM 243

Query: 245 RKNQERVIIRLTEARREAXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLELQLPHE 304
           +  Q+ V++ L        XXXXXXXXXXXXXXXXXXXXX   +V SYVDTLL+++LP +
Sbjct: 244 QSEQQDVLLPL--------XXXXXXXXXXXXXXXXXXXXXNKEYVQSYVDTLLDVELP-D 303

Query: 305 DNRKLTDGEIVTL 315
           + RKL + EIV+L
Sbjct: 304 EKRKLNEDEIVSL 306

BLAST of Bhi10G001301 vs. TAIR10
Match: AT5G61320.1 (cytochrome P450, family 89, subfamily A, polypeptide 3)

HSP 1 Score: 279.3 bits (713), Expect = 3.0e-75
Identity = 149/312 (47.76%), Postives = 201/312 (64.42%), Query Frame = 0

Query: 4   TWF-IILISLCICYLFSSIFIKFRSSPKLPPGPPSIPFISTFLWLRTSPLQMESYLRTAV 63
           TWF I  +S  + +L    F +  ++  LPP P   P    F WLR       SYLR+  
Sbjct: 3   TWFHIFCVSFTVNFLLYLFFRRTNNNLPLPPNPNFFPMPGPFQWLRQGFDDFYSYLRSIH 62

Query: 64  AKYGPIITLRIGSRPSIFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQHSINSAA 123
            + GPII+LRI S P+IF+ DR++AHKAL+ +G +F+DRPP  P  KI++SNQH+I+S +
Sbjct: 63  HRLGPIISLRIFSVPAIFVSDRSLAHKALVLNGAVFSDRPPALPTGKIITSNQHTISSGS 122

Query: 124 YGPLWRLLRRNLTSQILHPSRMRLYGRARKWVLDILLNRLLSHSESGSPVYVVDHFQYAM 183
           YG  WRLLRRNLTS+ILHPSR++ Y  AR+ VL+ L +R+ +H E   P+ VVDH +YAM
Sbjct: 123 YGATWRLLRRNLTSEILHPSRVKSYSNARRSVLENLCSRIRNHGEEAKPIVVVDHLRYAM 182

Query: 184 FCLLVLMCFGDKLDESQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRWEEFLQLR 243
           F LLVLMCFGDKLDE QIK+VE VQR  L+   RF +LN++P  TK+FL KRWEEFL  R
Sbjct: 183 FSLLVLMCFGDKLDEEQIKQVEFVQRRELITLPRFNILNVFPSFTKLFLRKRWEEFLTFR 242

Query: 244 KNQERVIIRLTEARREAXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLELQLPHED 303
           +  + V++ L  +RR+                          ++ SYVDTLL+L+LP ++
Sbjct: 243 REHKNVLLPLIRSRRK---------------IMIESKDSGKEYIQSYVDTLLDLELP-DE 298

Query: 304 NRKLTDGEIVTL 315
            RKL + EIV+L
Sbjct: 303 KRKLNEDEIVSL 298

BLAST of Bhi10G001301 vs. TAIR10
Match: AT3G03470.1 (cytochrome P450, family 87, subfamily A, polypeptide 9)

HSP 1 Score: 259.6 bits (662), Expect = 2.5e-69
Identity = 143/324 (44.14%), Postives = 204/324 (62.96%), Query Frame = 0

Query: 1   METTWFIILISLCICYLFSSIFIK---FRSSPKLPPGPPSIPFISTFLWLRTSPL-QMES 60
           ME T  I LI   I  L  SIF+K   F S+ KLPPGPP  P I   +WL+ +     + 
Sbjct: 1   MEITTIIFLI---ISSLTFSIFLKLIFFFSTHKLPPGPPRFPVIGNIIWLKKNNFSDFQG 60

Query: 61  YLRTAVAKYGPIITLRIGSRPSIFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQH 120
            LR   +++GPIITL +GS+PSI++ DR++AH+AL+Q+G +F+DR    P TK+++SNQH
Sbjct: 61  VLRDLASRHGPIITLHVGSKPSIWVTDRSLAHQALVQNGAVFSDRSLALPTTKVITSNQH 120

Query: 121 SINSAAYGPLWRLLRRNLTSQILHPSRMRLYGRARKWVLDILLNRL-LSHSESGSPVYVV 180
            I+S+ YG LWR LRRNLTS+IL PSR++ +  +RKW L+IL++       E G     +
Sbjct: 121 DIHSSVYGSLWRTLRRNLTSEILQPSRVKAHAPSRKWSLEILVDLFETEQREKGHISDAL 180

Query: 181 DHFQYAMFCLLVLMCFGDKLDESQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRW 240
           DH ++AMF LL LMCFG+KL + +I+E+E  Q  ML+++ +F +LN++P +TK  L ++W
Sbjct: 181 DHLRHAMFYLLALMCFGEKLRKEEIREIEEAQYQMLISYTKFSVLNIFPSVTKFLLRRKW 240

Query: 241 EEFLQLRKNQERVIIRLTEARREAXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLE 300
           +EFL+LRK+QE VI+R   AR +                           VL YVDTLL 
Sbjct: 241 KEFLELRKSQESVILRYVNARSKETTGD----------------------VLCYVDTLLN 299

Query: 301 LQLPHED-----NRKLTDGEIVTL 315
           L++P E+      RKL+D EIV+L
Sbjct: 301 LEIPTEEKEGGKKRKLSDSEIVSL 299

BLAST of Bhi10G001301 vs. Swiss-Prot
Match: sp|Q9SRQ1|C89A9_ARATH (Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 4.4e-68
Identity = 143/324 (44.14%), Postives = 204/324 (62.96%), Query Frame = 0

Query: 1   METTWFIILISLCICYLFSSIFIK---FRSSPKLPPGPPSIPFISTFLWLRTSPL-QMES 60
           ME T  I LI   I  L  SIF+K   F S+ KLPPGPP  P I   +WL+ +     + 
Sbjct: 1   MEITTIIFLI---ISSLTFSIFLKLIFFFSTHKLPPGPPRFPVIGNIIWLKKNNFSDFQG 60

Query: 61  YLRTAVAKYGPIITLRIGSRPSIFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQH 120
            LR   +++GPIITL +GS+PSI++ DR++AH+AL+Q+G +F+DR    P TK+++SNQH
Sbjct: 61  VLRDLASRHGPIITLHVGSKPSIWVTDRSLAHQALVQNGAVFSDRSLALPTTKVITSNQH 120

Query: 121 SINSAAYGPLWRLLRRNLTSQILHPSRMRLYGRARKWVLDILLNRL-LSHSESGSPVYVV 180
            I+S+ YG LWR LRRNLTS+IL PSR++ +  +RKW L+IL++       E G     +
Sbjct: 121 DIHSSVYGSLWRTLRRNLTSEILQPSRVKAHAPSRKWSLEILVDLFETEQREKGHISDAL 180

Query: 181 DHFQYAMFCLLVLMCFGDKLDESQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRW 240
           DH ++AMF LL LMCFG+KL + +I+E+E  Q  ML+++ +F +LN++P +TK  L ++W
Sbjct: 181 DHLRHAMFYLLALMCFGEKLRKEEIREIEEAQYQMLISYTKFSVLNIFPSVTKFLLRRKW 240

Query: 241 EEFLQLRKNQERVIIRLTEARREAXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLE 300
           +EFL+LRK+QE VI+R   AR +                           VL YVDTLL 
Sbjct: 241 KEFLELRKSQESVILRYVNARSKETTGD----------------------VLCYVDTLLN 299

Query: 301 LQLPHED-----NRKLTDGEIVTL 315
           L++P E+      RKL+D EIV+L
Sbjct: 301 LEIPTEEKEGGKKRKLSDSEIVSL 299

BLAST of Bhi10G001301 vs. Swiss-Prot
Match: sp|Q42602|C89A2_ARATH (Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2)

HSP 1 Score: 188.3 bits (477), Expect = 1.3e-46
Identity = 107/282 (37.94%), Postives = 152/282 (53.90%), Query Frame = 0

Query: 33  PGPPSIPFISTFLWLRTSPLQMESYLRTAVAKYGPIITLRIGSRPSIFIVDRTIAHKALL 92
           P P  +PF+ T  WLR     +ESYLR+   + GPI+TLRI SRP+IF+ DR++ H+AL+
Sbjct: 33  PDPNFLPFLGTLQWLREGLGGLESYLRSVHHRLGPIVTLRITSRPAIFVADRSLTHEALV 92

Query: 93  QHGTLFADRPPPPPLTKILSSNQHSINSAAYGPLWRLLRRNLTSQILHPSRMRLYGRARK 152
            +G ++ADRPPP  ++KI+  ++H+I+S +YG  WRLLRRN+TS+ILHPSR+R Y  AR 
Sbjct: 93  LNGAVYADRPPPAVISKIV--DEHNISSGSYGATWRLLRRNITSEILHPSRVRSYSHARH 152

Query: 153 WVLDILLNRLLSHSESGSPVYVVDHFQYAMFCLLVLMCFGDKLDESQIKEVENVQRTMLL 212
           WVL+IL  R  +H     P+ ++ H                                   
Sbjct: 153 WVLEILFERFRNHG-GEEPIVLIHHXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 212

Query: 213 NFMRFGLLNLYPKLTKIFLPKRWEEFLQLRKNQERVIIRLTEARREAXXXXXXXXXXXXX 272
                         TK+ L KRW+EFLQ+R+ Q  V++ L  ARR+              
Sbjct: 213 XXXXXXXXXXXXXXTKLILRKRWQEFLQIRRQQRDVLLPLIRARRK-----------IVE 272

Query: 273 XXXXXXXXXXXXFVLSYVDTLLELQLPHEDNRKLTDGEIVTL 315
                       +V SYVDTLL+L+LP E+NRKL + +I+ L
Sbjct: 273 ERKRSEQEDKKDYVQSYVDTLLDLELP-EENRKLNEEDIMNL 299

BLAST of Bhi10G001301 vs. Swiss-Prot
Match: sp|Q9LZ31|C77A4_ARATH (Cytochrome P450 77A4 OS=Arabidopsis thaliana OX=3702 GN=CYP77A4 PE=2 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.1e-37
Identity = 100/321 (31.15%), Postives = 168/321 (52.34%), Query Frame = 0

Query: 1   METTWFIILISLCICYLFSSIFIKFRSSPK----LPPGPPSIPFISTFLWLRTSPLQMES 60
           ++ T+F I+IS      F  I  ++ S+ K    LPPGPP  P +        S      
Sbjct: 12  LDFTFFAIIIS-----GFVFIITRWNSNSKKRLNLPPGPPGWPVVGNLFQFARSGKPFFE 71

Query: 61  YLRTAVAKYGPIITLRIGSRPSIFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQH 120
           Y       YGPI TLR+G+R  I + D T+ H+AL+Q G LFA RP   P   I S N+ 
Sbjct: 72  YAEDLKKTYGPIFTLRMGTRTMIILSDATLVHEALIQRGALFASRPAENPTRTIFSCNKF 131

Query: 121 SINSAAYGPLWRLLRRNLTSQILHPSRMRLYGRARKWVLDILLNRLLSHS-ESGSPVYVV 180
           ++N+A YGP+WR LRRN+   +L  +R++ +G+ R+  +D L+ R+ S + ++   ++V+
Sbjct: 132 TVNAAKYGPVWRSLRRNMVQNMLSSTRLKEFGKLRQSAMDKLIERIKSEARDNDGLIWVL 191

Query: 181 DHFQYAMFCLLVLMCFGDKLDESQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRW 240
            + ++A FC+L+ MCFG ++DE  I++++ + +T+L+       ++ Y  +   F  K  
Sbjct: 192 KNARFAAFCILLEMCFGIEMDEETIEKMDEILKTVLMTV--DPRIDDYLPILAPFFSKER 251

Query: 241 EEFLQLRKNQERVIIRLTEARREAXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLE 300
           +  L++R+ Q   ++ + E RR A                            SY+DTL +
Sbjct: 252 KRALEVRREQVDYVVGVIERRRRAIQNPGSDKTASS---------------FSYLDTLFD 307

Query: 301 LQLPHEDNRKLT--DGEIVTL 315
           L++   + RK T  + E+VTL
Sbjct: 312 LKI---EGRKTTPSNEELVTL 307

BLAST of Bhi10G001301 vs. Swiss-Prot
Match: sp|O48928|C77A3_SOYBN (Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 5.3e-37
Identity = 89/297 (29.97%), Postives = 156/297 (52.53%), Query Frame = 0

Query: 22  FIKFRSSPK---LPPGPPSIPFISTFLWLRTSPLQMESYLRTAVAKYGPIITLRIGSRPS 81
           F+K +S  K   LPPGPP  P +     +  S      Y+     KYG I TL++G+R  
Sbjct: 25  FLKQKSKSKKFNLPPGPPGWPIVGNLFQVARSGKPFFEYVNDVRLKYGSIFTLKMGTRTM 84

Query: 82  IFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQHSINSAAYGPLWRLLRRNLTSQI 141
           I + D  + H+A++Q G  +A RPP  P   I S N+ ++N+A YGP+W+ LRRN+   +
Sbjct: 85  IILTDAKLVHEAMIQKGATYATRPPENPTRTIFSENKFTVNAATYGPVWKSLRRNMVQNM 144

Query: 142 LHPSRMRLYGRARKWVLDILLNRLLSHSE-SGSPVYVVDHFQYAMFCLLVLMCFGDKLDE 201
           L  +R++ +   R   +D L+NRL   +E +   V+V+   ++A+FC+LV MCFG ++DE
Sbjct: 145 LSSTRLKEFRSVRDNAMDKLINRLKDEAEKNNGVVWVLKDARFAVFCILVAMCFGLEMDE 204

Query: 202 SQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRWEEFLQLRKNQERVIIRLTEARR 261
             ++ ++ V +++L+       ++ Y  +   F  K+ ++ L++R+ Q   ++ + E RR
Sbjct: 205 ETVERIDQVMKSVLITL--DPRIDDYLPILSPFFSKQRKKALEVRREQVEFLVPIIEQRR 264

Query: 262 EAXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLELQLPHEDNRKLTDGEIVTL 315
            A                            SY+DTL +L++  + +   +D E+V+L
Sbjct: 265 RAIQNPGSDHTAT---------------TFSYLDTLFDLKVEGKKSAP-SDAELVSL 303

BLAST of Bhi10G001301 vs. Swiss-Prot
Match: sp|P37123|C77A1_SOLME (Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 7.2e-34
Identity = 101/315 (32.06%), Postives = 160/315 (50.79%), Query Frame = 0

Query: 4   TWFIILISLCICYLFSSIFIKFRSSPKLPPGPPSIPFISTFLWLRTSPLQMESYLRTAVA 63
           T F +L SL I  L      K   +P LPPGPP  P +     +  S  Q   Y+R    
Sbjct: 3   TAFSLLFSLFIFLLTRKPKSK---TPNLPPGPPGWPIVGNLFQVAGSGKQFFEYIRDLKP 62

Query: 64  KYGPIITLRIGSRPSIFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQHSINSAAY 123
           KYG I TL++GSR  I +    +AH+AL+Q G +FA RP   P   I S N+ S+N+A Y
Sbjct: 63  KYGSIFTLKMGSRTMIIVASAELAHEALIQKGQIFASRPRENPTRTIFSCNKFSVNAAVY 122

Query: 124 GPLWRLLRRNLTSQILHPSRMRLYGRARKWVLDILLNRL-LSHSESGSPVYVVDHFQYAM 183
           GP+WR LRRN+   +L PSR++ +   R+  +D L+ R+ +   E+   V+ + + ++A+
Sbjct: 123 GPVWRSLRRNMVQNMLSPSRLKEFREFREIAMDKLIERIRVDAKENNDVVWALKNARFAV 182

Query: 184 FCLLVLMCFGDKLD-ESQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRWEEFLQL 243
           F +LV MCFG ++D E  I+ V+ + + +L+  +   + +  P L      K+ +   ++
Sbjct: 183 FYILVAMCFGVEMDNEEMIERVDQMMKDVLI-VLDPRIDDFLPILRLFVGYKQRKRVNEV 242

Query: 244 RKNQERVIIRLTEARREAXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLELQLPHE 303
           RK Q   ++ L E RR                              SY+DTL ++++   
Sbjct: 243 RKRQIETLVPLIEKRRSVVQNPGSDKTAAS---------------FSYLDTLFDVKV--- 295

Query: 304 DNRKL--TDGEIVTL 315
           + RK   T+ E+VTL
Sbjct: 303 EGRKSGPTNAELVTL 295

BLAST of Bhi10G001301 vs. TrEMBL
Match: tr|A0A0A0LNK3|A0A0A0LNK3_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G437040 PE=3 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 4.5e-134
Identity = 250/314 (79.62%), Postives = 265/314 (84.39%), Query Frame = 0

Query: 1   METTWFIILISLCICYLFSSIFIKFRSSPKLPPGPPSIPFISTFLWLRTSPLQMESYLRT 60
           M+T + IILIS  ICYL SSIF KF++S KLPPGPPSIP +STFLW RTSPLQMESYLRT
Sbjct: 33  MDTWFIIILISFSICYLLSSIFTKFQTSTKLPPGPPSIPILSTFLWFRTSPLQMESYLRT 92

Query: 61  AVAKYGPIITLRIGSRPSIFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQHSINS 120
           AVAKYGPI+TLRIGSRPSIFI DRTIAHKAL QHG LFADRPP PPLTKIL+SNQHSINS
Sbjct: 93  AVAKYGPIVTLRIGSRPSIFIADRTIAHKALFQHGALFADRPPVPPLTKILTSNQHSINS 152

Query: 121 AAYGPLWRLLRRNLTSQILHPSRMRLYGRARKWVLDILLNRLLSHSESGSPVYVVDHFQY 180
           AAYGPLWRLLRRNLTSQILHPSR+R YG AR+WVL ILLNRL SHSESGS VYVVDHFQY
Sbjct: 153 AAYGPLWRLLRRNLTSQILHPSRIRSYGHAREWVLGILLNRLFSHSESGSSVYVVDHFQY 212

Query: 181 AMFCLLVLMCFGDKLDESQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRWEEFLQ 240
           AMFCL+VLMCFGDKL+ESQIKEVENVQRTMLLNF RFGLLNL PKLTK FLPKRWEEFLQ
Sbjct: 213 AMFCLMVLMCFGDKLEESQIKEVENVQRTMLLNFRRFGLLNLSPKLTKFFLPKRWEEFLQ 272

Query: 241 LRKNQERVIIRLTEARREAXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLELQLPH 300
           LR+NQERVII L EARREA                         FVLSYVDTLLELQLP+
Sbjct: 273 LRRNQERVIIPLIEARREA---IKSRANRGKREGEKQEQEDGKEFVLSYVDTLLELQLPN 332

Query: 301 EDNRKLTDGEIVTL 315
           EDNRKLT+ E+VTL
Sbjct: 333 EDNRKLTNSEMVTL 343

BLAST of Bhi10G001301 vs. TrEMBL
Match: tr|Q6E439|Q6E439_CUCME (ACT11D09.3 OS=Cucumis melo OX=3656 GN=ACT11D09.3 PE=3 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 9.5e-108
Identity = 202/310 (65.16%), Postives = 242/310 (78.06%), Query Frame = 0

Query: 5   WFIILISLCICYLFSSIFIKFRSSPKLPPGPPSIPFISTFLWLRTSPLQMESYLRTAVAK 64
           WFI LISL IC L +SIF  F++S KLPPGPPSIP ++ FLWLR S LQ+ES LR+ VAK
Sbjct: 4   WFIFLISLSICSLLTSIFTHFQTSTKLPPGPPSIPILTNFLWLRRSSLQIESLLRSFVAK 63

Query: 65  YGPIITLRIGSRPSIFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQHSINSAAYG 124
           YGP++TLRIGSRP++FI DR+IAHK L+Q+G LFADRPP   + K+++SNQH+I+SA+YG
Sbjct: 64  YGPVLTLRIGSRPTVFIADRSIAHKILVQNGALFADRPPALSVGKVITSNQHNISSASYG 123

Query: 125 PLWRLLRRNLTSQILHPSRMRLYGRARKWVLDILLNRLLSHSESGSPVYVVDHFQYAMFC 184
           PLWRLLRRNLTSQILHPSR+R Y  ARKWVLDILLNRL S SESG+PV V+++FQYAMFC
Sbjct: 124 PLWRLLRRNLTSQILHPSRVRSYSEARKWVLDILLNRLQSQSESGNPVSVIENFQYAMFC 183

Query: 185 LLVLMCFGDKLDESQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRWEEFLQLRKN 244
           LLVLMCFGDKLDESQI+EVENV+R M+L+F RF +LN +PK TKI   KRWE F QLRKN
Sbjct: 184 LLVLMCFGDKLDESQIREVENVERAMILSFQRFNILNFWPKFTKILFRKRWEAFFQLRKN 243

Query: 245 QERVIIRLTEARREAXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLELQLPHEDNR 304
           QE+V+ RL EARR+A                          V+SYVDTLLEL+LP ++ R
Sbjct: 244 QEKVLTRLIEARRKA-----------NGNRENKAQNEEEEIVVSYVDTLLELELP-DEKR 301

Query: 305 KLTDGEIVTL 315
           KL D E+VTL
Sbjct: 304 KLNDDELVTL 301

BLAST of Bhi10G001301 vs. TrEMBL
Match: tr|A0A1S3CAR2|A0A1S3CAR2_CUCME (cytochrome P450 89A2-like OS=Cucumis melo OX=3656 GN=LOC103498359 PE=3 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 6.2e-107
Identity = 217/302 (71.85%), Postives = 252/302 (83.44%), Query Frame = 0

Query: 13  CICYLFSSIFIKFRSSPKLPPGPPSIPFISTFLWLRTSPLQMESYLRTAVAKYGPIITLR 72
           CIC L +SIF  FRSS KLPPGPPSIP ++   WLR SPLQMES LRT V+KYGPIITL 
Sbjct: 29  CICSLLNSIFSHFRSSTKLPPGPPSIPILTNLRWLRKSPLQMESLLRTFVSKYGPIITLP 88

Query: 73  IGSRPSIFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQHSINSAAYGPLWRLLRR 132
           IGSRP++FI DR+IAHKAL+ +G LFADRPP PP+TKI SSNQH+IN+A+YGPLWRLLRR
Sbjct: 89  IGSRPAVFIADRSIAHKALVLNGALFADRPPAPPVTKIASSNQHNINTASYGPLWRLLRR 148

Query: 133 NLTSQILHPSRMRLYGRARKWVLDILLNRLLSHSESGSPVYVVDHFQYAMFCLLVLMCFG 192
           NLTSQILHPSR++ YGRARKWVLD+L+NR +SHS+SG+PV VVDHFQYAMFCLLVLMCFG
Sbjct: 149 NLTSQILHPSRVKSYGRARKWVLDVLINRFVSHSQSGNPVCVVDHFQYAMFCLLVLMCFG 208

Query: 193 DKLDESQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRWEEFLQLRKNQERVIIRL 252
           DKL+ESQIKE+ENV  TML+N  RF +LN +P LTKI L KRWE FL+L+ N++ V+I L
Sbjct: 209 DKLNESQIKEIENVHHTMLINLRRFNVLNFWPILTKILLRKRWETFLKLKNNRDEVLIPL 268

Query: 253 TEARREAXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLELQLPHEDNRKLTDGEIV 312
            EARR          XXXXXXXXXXXXXXXXXFV+SYVDTLL+L+ P E+ RKLTD EIV
Sbjct: 269 IEARR----------XXXXXXXXXXXXXXXXXFVVSYVDTLLDLEHP-EEKRKLTDEEIV 319

Query: 313 TL 315
            +
Sbjct: 329 AI 319

BLAST of Bhi10G001301 vs. TrEMBL
Match: tr|A0A0A0LNB1|A0A0A0LNB1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G435530 PE=3 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.1e-106
Identity = 197/310 (63.55%), Postives = 240/310 (77.42%), Query Frame = 0

Query: 5   WFIILISLCICYLFSSIFIKFRSSPKLPPGPPSIPFISTFLWLRTSPLQMESYLRTAVAK 64
           WF+IL+SLCI  L +SIF  FR+S  LPPGPPSIP  + F WLR SPLQ+ES LR+ V K
Sbjct: 4   WFVILVSLCISSLLTSIFTHFRTSSNLPPGPPSIPIFTNFQWLRRSPLQIESLLRSFVTK 63

Query: 65  YGPIITLRIGSRPSIFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQHSINSAAYG 124
           YGP++TL IG+ P +FI DR+IAHKAL +HG LFADRPP PPL+K++SSNQ+SI+SA+YG
Sbjct: 64  YGPVVTLPIGNHPVVFIADRSIAHKALFKHGALFADRPPAPPLSKVISSNQYSISSASYG 123

Query: 125 PLWRLLRRNLTSQILHPSRMRLYGRARKWVLDILLNRLLSHSESGSPVYVVDHFQYAMFC 184
           PLWRLLRRNLTSQILH SR+  Y +AR+WVLDILL+RL SHS+S  PV VV++FQYAMFC
Sbjct: 124 PLWRLLRRNLTSQILHSSRIVFYSQARRWVLDILLSRLQSHSQSKIPVLVVENFQYAMFC 183

Query: 185 LLVLMCFGDKLDESQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRWEEFLQLRKN 244
           LLVLMCFGDKL+ESQI+EVE V+R ++L+F RF + N +PK TKI L KRWE FLQ+RKN
Sbjct: 184 LLVLMCFGDKLEESQIREVEKVERELILSFQRFNIFNFWPKFTKILLRKRWEAFLQIRKN 243

Query: 245 QERVIIRLTEARREAXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLELQLPHEDNR 304
           QE V+ RL E RR+A                         FV+SYVDTLL+L+LP+EDNR
Sbjct: 244 QENVLNRLIEERRKA----------NKNRANKAQNEEKEEFVVSYVDTLLDLELPNEDNR 303

Query: 305 KLTDGEIVTL 315
           KL++ EIVTL
Sbjct: 304 KLSNEEIVTL 303

BLAST of Bhi10G001301 vs. TrEMBL
Match: tr|A0A0A0LNJ6|A0A0A0LNJ6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G435510 PE=3 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 5.2e-106
Identity = 198/310 (63.87%), Postives = 243/310 (78.39%), Query Frame = 0

Query: 5   WFIILISLCICYLFSSIFIKFRSSPKLPPGPPSIPFISTFLWLRTSPLQMESYLRTAVAK 64
           WFI +ISL +C L +SIF  F++S KLPPGPPSIP ++ FLWLR S LQ+ES LR+ VAK
Sbjct: 4   WFIFIISLSLCSLLTSIFTHFQTSTKLPPGPPSIPILTNFLWLRRSSLQIESLLRSFVAK 63

Query: 65  YGPIITLRIGSRPSIFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQHSINSAAYG 124
           YGP++TLRIGSRP++FI DR+IAHK L+Q+G LFADRPP   ++K+++SNQH+I+SA+YG
Sbjct: 64  YGPVLTLRIGSRPTVFIADRSIAHKILVQNGALFADRPPALSVSKVVTSNQHNISSASYG 123

Query: 125 PLWRLLRRNLTSQILHPSRMRLYGRARKWVLDILLNRLLSHSESGSPVYVVDHFQYAMFC 184
           PLWRLLRRNLTSQILHPSR+R Y  ARKWVLDILLNRL S SESG+PV VV++FQYAMFC
Sbjct: 124 PLWRLLRRNLTSQILHPSRVRSYSEARKWVLDILLNRLQSQSESGNPVSVVENFQYAMFC 183

Query: 185 LLVLMCFGDKLDESQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRWEEFLQLRKN 244
           LLVLMCFGDKL+ESQI+EVENV+R ++L F RF +LN +PK+TKI   KRWE F QLRKN
Sbjct: 184 LLVLMCFGDKLEESQIREVENVERQLILCFQRFNILNFWPKVTKILFRKRWEAFFQLRKN 243

Query: 245 QERVIIRLTEARREAXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLELQLPHEDNR 304
           QE+V+ RL +ARR+A                          V+SYVDTLLEL+LP ++ R
Sbjct: 244 QEKVLTRLIDARRKA--------------NENRAQNEEEEIVVSYVDTLLELELP-DEKR 298

Query: 305 KLTDGEIVTL 315
           KL D E+VTL
Sbjct: 304 KLNDDELVTL 298

BLAST of Bhi10G001301 vs. NCBI nr
Match: XP_011650075.1 (PREDICTED: cytochrome P450 89A2-like [Cucumis sativus] >KGN63393.1 hypothetical protein Csa_2G437040 [Cucumis sativus])

HSP 1 Score: 486.5 bits (1251), Expect = 6.8e-134
Identity = 250/314 (79.62%), Postives = 265/314 (84.39%), Query Frame = 0

Query: 1   METTWFIILISLCICYLFSSIFIKFRSSPKLPPGPPSIPFISTFLWLRTSPLQMESYLRT 60
           M+T + IILIS  ICYL SSIF KF++S KLPPGPPSIP +STFLW RTSPLQMESYLRT
Sbjct: 33  MDTWFIIILISFSICYLLSSIFTKFQTSTKLPPGPPSIPILSTFLWFRTSPLQMESYLRT 92

Query: 61  AVAKYGPIITLRIGSRPSIFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQHSINS 120
           AVAKYGPI+TLRIGSRPSIFI DRTIAHKAL QHG LFADRPP PPLTKIL+SNQHSINS
Sbjct: 93  AVAKYGPIVTLRIGSRPSIFIADRTIAHKALFQHGALFADRPPVPPLTKILTSNQHSINS 152

Query: 121 AAYGPLWRLLRRNLTSQILHPSRMRLYGRARKWVLDILLNRLLSHSESGSPVYVVDHFQY 180
           AAYGPLWRLLRRNLTSQILHPSR+R YG AR+WVL ILLNRL SHSESGS VYVVDHFQY
Sbjct: 153 AAYGPLWRLLRRNLTSQILHPSRIRSYGHAREWVLGILLNRLFSHSESGSSVYVVDHFQY 212

Query: 181 AMFCLLVLMCFGDKLDESQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRWEEFLQ 240
           AMFCL+VLMCFGDKL+ESQIKEVENVQRTMLLNF RFGLLNL PKLTK FLPKRWEEFLQ
Sbjct: 213 AMFCLMVLMCFGDKLEESQIKEVENVQRTMLLNFRRFGLLNLSPKLTKFFLPKRWEEFLQ 272

Query: 241 LRKNQERVIIRLTEARREAXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLELQLPH 300
           LR+NQERVII L EARREA                         FVLSYVDTLLELQLP+
Sbjct: 273 LRRNQERVIIPLIEARREA---IKSRANRGKREGEKQEQEDGKEFVLSYVDTLLELQLPN 332

Query: 301 EDNRKLTDGEIVTL 315
           EDNRKLT+ E+VTL
Sbjct: 333 EDNRKLTNSEMVTL 343

BLAST of Bhi10G001301 vs. NCBI nr
Match: XP_022944211.1 (cytochrome P450 89A2-like [Cucurbita moschata])

HSP 1 Score: 470.3 bits (1209), Expect = 5.1e-129
Identity = 269/310 (86.77%), Postives = 278/310 (89.68%), Query Frame = 0

Query: 5   WFIILISLCICYLFSSIFIKFRSSPKLPPGPPSIPFISTFLWLRTSPLQMESYLRTAVAK 64
           WFIILISLCICY+ SSIF  FRSS KLPPGPPSIP ISTFLWLRTSPLQMESYLRTAVAK
Sbjct: 4   WFIILISLCICYVLSSIFSHFRSSTKLPPGPPSIPIISTFLWLRTSPLQMESYLRTAVAK 63

Query: 65  YGPIITLRIGSRPSIFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQHSINSAAYG 124
           YGPI+TLRIGSR SIFI DRTIAH ALLQHG LFADRPP PPL KILSSNQHSINSA YG
Sbjct: 64  YGPIVTLRIGSRISIFIADRTIAHNALLQHGALFADRPPVPPLVKILSSNQHSINSAPYG 123

Query: 125 PLWRLLRRNLTSQILHPSRMRLYGRARKWVLDILLNRLLSHSESGSPVYVVDHFQYAMFC 184
           PLWRL RRNLTSQILHPSR+R Y  ARKWVLDILL RLL HSESG+PV VV HFQYAMFC
Sbjct: 124 PLWRLFRRNLTSQILHPSRVRSYAPARKWVLDILLKRLLPHSESGTPVCVVHHFQYAMFC 183

Query: 185 LLVLMCFGDKLDESQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRWEEFLQLRKN 244
           LLVLMCFGDKLDE +IKEVENVQRTMLLN  RFGLLNL PKLTKIFLPKRWEEFLQLR+N
Sbjct: 184 LLVLMCFGDKLDEPKIKEVENVQRTMLLNLRRFGLLNLSPKLTKIFLPKRWEEFLQLRRN 243

Query: 245 QERVIIRLTEARREAXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLELQLPHEDNR 304
           QERVII L EARR+  XXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLEL LP E NR
Sbjct: 244 QERVIIPLIEARRK-DXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLELHLPEEGNR 303

Query: 305 KLTDGEIVTL 315
           K+TDGE+V L
Sbjct: 304 KVTDGEMVGL 312

BLAST of Bhi10G001301 vs. NCBI nr
Match: XP_022986503.1 (cytochrome P450 89A2-like [Cucurbita maxima])

HSP 1 Score: 469.2 bits (1206), Expect = 1.1e-128
Identity = 268/310 (86.45%), Postives = 279/310 (90.00%), Query Frame = 0

Query: 5   WFIILISLCICYLFSSIFIKFRSSPKLPPGPPSIPFISTFLWLRTSPLQMESYLRTAVAK 64
           WFIILISLCICY+ SSIF  F+SS KLPPGPPSIP ISTFLWL TSPLQMESYLRTAVAK
Sbjct: 4   WFIILISLCICYVLSSIFSHFQSSTKLPPGPPSIPIISTFLWLHTSPLQMESYLRTAVAK 63

Query: 65  YGPIITLRIGSRPSIFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQHSINSAAYG 124
           YGPI+TLRIGSR SIFI DRTIAH ALLQHG LFADRPP PPL KILSSNQHSINSA YG
Sbjct: 64  YGPIVTLRIGSRISIFIADRTIAHNALLQHGALFADRPPVPPLVKILSSNQHSINSAPYG 123

Query: 125 PLWRLLRRNLTSQILHPSRMRLYGRARKWVLDILLNRLLSHSESGSPVYVVDHFQYAMFC 184
           PLWRL RRNLTSQILHPSR+R Y  ARKWVLDILL RLL +SES SPV VV+HFQYAMFC
Sbjct: 124 PLWRLFRRNLTSQILHPSRVRSYAPARKWVLDILLKRLLPNSESVSPVCVVEHFQYAMFC 183

Query: 185 LLVLMCFGDKLDESQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRWEEFLQLRKN 244
           LLVLMCFGDKLDE +IKEVENVQRTMLLN  RFGLLNL PKLTKIFLPKRWEEFLQLR+N
Sbjct: 184 LLVLMCFGDKLDEPKIKEVENVQRTMLLNLRRFGLLNLSPKLTKIFLPKRWEEFLQLRRN 243

Query: 245 QERVIIRLTEARREAXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLELQLPHEDNR 304
           QERVII L EARR+AXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLEL LP E NR
Sbjct: 244 QERVIIPLIEARRKAXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLELHLPEEGNR 303

Query: 305 KLTDGEIVTL 315
           K+TDGE+V L
Sbjct: 304 KVTDGEMVGL 313

BLAST of Bhi10G001301 vs. NCBI nr
Match: XP_022943742.1 (cytochrome P450 89A2-like isoform X1 [Cucurbita moschata] >XP_022943743.1 cytochrome P450 89A2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 468.4 bits (1204), Expect = 1.9e-128
Identity = 268/316 (84.81%), Postives = 283/316 (89.56%), Query Frame = 0

Query: 5   WFIILISLCICYLFSSIFIKFRSSPKLPPGPPSIPFISTFLWLRTSPLQMESYLRTAVAK 64
           WFIIL+SLCI Y+ SSI   FRSS KLPPGPPSIP ISTFLWLRTSPLQ+ESYLRTAVAK
Sbjct: 4   WFIILVSLCISYVLSSILSHFRSSTKLPPGPPSIPIISTFLWLRTSPLQLESYLRTAVAK 63

Query: 65  YGPIITLRIGSRPSIFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQHSINSAAYG 124
           YGPI+TLRIGSR SIFI DRTIAH ALLQHG LFADRPPPPPLTKIL SNQHSI+SA YG
Sbjct: 64  YGPIVTLRIGSRISIFIADRTIAHNALLQHGALFADRPPPPPLTKILFSNQHSIHSAPYG 123

Query: 125 PLWRLLRRNLTSQILHPSRMRLYGRARKWVLDILLNRLLSHSESGSPVYVVDHFQYAMFC 184
           PLWRLLRRNLTSQILHPSR+R Y  ARKWVLDILL RLLSHSESG+PV V DHFQYAMFC
Sbjct: 124 PLWRLLRRNLTSQILHPSRVRSYAPARKWVLDILLKRLLSHSESGTPVCVADHFQYAMFC 183

Query: 185 LLVLMCFGDKLDESQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRWEEFLQLRKN 244
           LLVLMCFGDKLDE +IKEVENVQRTMLLN  RFGLLNL PKLTKIFLPKRWEEFLQLRKN
Sbjct: 184 LLVLMCFGDKLDEPKIKEVENVQRTMLLNLSRFGLLNLSPKLTKIFLPKRWEEFLQLRKN 243

Query: 245 QERVIIRLTEARREA------XXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLELQL 304
           QERVII L EARR+A      XXXXXXXXXXXXXXXXXXXXXXXXXFV+SYVDTLL+L++
Sbjct: 244 QERVIIPLIEARRKANQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFVVSYVDTLLDLEI 303

Query: 305 PHEDNRKLTDGEIVTL 315
           P EDNRKLT+ EIVT+
Sbjct: 304 PDEDNRKLTNEEIVTI 319

BLAST of Bhi10G001301 vs. NCBI nr
Match: XP_022986505.1 (cytochrome P450 89A2-like [Cucurbita maxima])

HSP 1 Score: 465.3 bits (1196), Expect = 1.6e-127
Identity = 245/310 (79.03%), Postives = 260/310 (83.87%), Query Frame = 0

Query: 5   WFIILISLCICYLFSSIFIKFRSSPKLPPGPPSIPFISTFLWLRTSPLQMESYLRTAVAK 64
           WFIIL+SLCI YL SSIF  F SS KLPPGPPSIP ISTFLWLRTSPLQ+ESYLRTAVAK
Sbjct: 4   WFIILVSLCISYLLSSIFSHFGSSTKLPPGPPSIPIISTFLWLRTSPLQLESYLRTAVAK 63

Query: 65  YGPIITLRIGSRPSIFIVDRTIAHKALLQHGTLFADRPPPPPLTKILSSNQHSINSAAYG 124
           YGPI+TLRIGSR SIFI DRTIAH ALLQHG LFADRPPPPPLTKIL SNQHSI+SA YG
Sbjct: 64  YGPIVTLRIGSRISIFIADRTIAHNALLQHGALFADRPPPPPLTKILFSNQHSIHSAPYG 123

Query: 125 PLWRLLRRNLTSQILHPSRMRLYGRARKWVLDILLNRLLSHSESGSPVYVVDHFQYAMFC 184
           PLWRLLRRNLTSQILHPSR+R Y  ARKWVLDILL RL SHSESG+PV VVDHFQYAMFC
Sbjct: 124 PLWRLLRRNLTSQILHPSRVRSYAPARKWVLDILLKRLHSHSESGTPVCVVDHFQYAMFC 183

Query: 185 LLVLMCFGDKLDESQIKEVENVQRTMLLNFMRFGLLNLYPKLTKIFLPKRWEEFLQLRKN 244
           LLVLMCFGDKLDE +IKEVENVQRTMLL+  RFGLLN  PKLTKIFLPKRWEEFLQLRK+
Sbjct: 184 LLVLMCFGDKLDEPKIKEVENVQRTMLLSLSRFGLLNSSPKLTKIFLPKRWEEFLQLRKD 243

Query: 245 QERVIIRLTEARREAXXXXXXXXXXXXXXXXXXXXXXXXXFVLSYVDTLLELQLPHEDNR 304
           QERVII L EARR+A               XXX       FVLSYVDTLLEL+ P E+NR
Sbjct: 244 QERVIIPLIEARRKA------KQNRGNREEXXXGKEEDEDFVLSYVDTLLELRHPEEENR 303

Query: 305 KLTDGEIVTL 315
           K+TDGE+V+L
Sbjct: 304 KITDGEMVSL 307

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G64950.13.7e-8159.29cytochrome P450, family 89, subfamily A, polypeptide 5[more]
AT2G12190.15.3e-8058.01Cytochrome P450 superfamily protein[more]
AT1G64940.13.6e-7655.91cytochrome P450, family 87, subfamily A, polypeptide 6[more]
AT5G61320.13.0e-7547.76cytochrome P450, family 89, subfamily A, polypeptide 3[more]
AT3G03470.12.5e-6944.14cytochrome P450, family 87, subfamily A, polypeptide 9[more]
Match NameE-valueIdentityDescription
sp|Q9SRQ1|C89A9_ARATH4.4e-6844.14Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1[more]
sp|Q42602|C89A2_ARATH1.3e-4637.94Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2[more]
sp|Q9LZ31|C77A4_ARATH3.1e-3731.15Cytochrome P450 77A4 OS=Arabidopsis thaliana OX=3702 GN=CYP77A4 PE=2 SV=1[more]
sp|O48928|C77A3_SOYBN5.3e-3729.97Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1[more]
sp|P37123|C77A1_SOLME7.2e-3432.06Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
tr|A0A0A0LNK3|A0A0A0LNK3_CUCSA4.5e-13479.62Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G437040 PE=3 SV=1[more]
tr|Q6E439|Q6E439_CUCME9.5e-10865.16ACT11D09.3 OS=Cucumis melo OX=3656 GN=ACT11D09.3 PE=3 SV=1[more]
tr|A0A1S3CAR2|A0A1S3CAR2_CUCME6.2e-10771.85cytochrome P450 89A2-like OS=Cucumis melo OX=3656 GN=LOC103498359 PE=3 SV=1[more]
tr|A0A0A0LNB1|A0A0A0LNB1_CUCSA1.1e-10663.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G435530 PE=3 SV=1[more]
tr|A0A0A0LNJ6|A0A0A0LNJ6_CUCSA5.2e-10663.87Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G435510 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_011650075.16.8e-13479.62PREDICTED: cytochrome P450 89A2-like [Cucumis sativus] >KGN63393.1 hypothetical ... [more]
XP_022944211.15.1e-12986.77cytochrome P450 89A2-like [Cucurbita moschata][more]
XP_022986503.11.1e-12886.45cytochrome P450 89A2-like [Cucurbita maxima][more]
XP_022943742.11.9e-12884.81cytochrome P450 89A2-like isoform X1 [Cucurbita moschata] >XP_022943743.1 cytoch... [more]
XP_022986505.11.6e-12779.03cytochrome P450 89A2-like [Cucurbita maxima][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037heme binding
GO:0005506iron ion binding
Vocabulary: INTERPRO
TermDefinition
IPR001128Cyt_P450
IPR036396Cyt_P450_sf
IPR002401Cyt_P450_E_grp-I
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi10M001301Bhi10M001301mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 242..262
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 261..275
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 261..283
NoneNo IPR availablePANTHERPTHR24298FAMILY NOT NAMEDcoord: 4..314
NoneNo IPR availablePANTHERPTHR24298:SF45CYTOCHROME P450 89A2-RELATEDcoord: 4..314
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 181..199
score: 15.53
coord: 62..81
score: 32.67
coord: 86..107
score: 26.77
IPR036396Cytochrome P450 superfamilyGENE3DG3DSA:1.10.630.10coord: 25..314
e-value: 4.1E-35
score: 123.4
IPR036396Cytochrome P450 superfamilySUPERFAMILYSSF48264Cytochrome P450coord: 23..312
IPR001128Cytochrome P450PFAMPF00067p450coord: 32..312
e-value: 4.1E-27
score: 95.0