Bhi10G001300 (gene) Wax gourd

NameBhi10G001300
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionCytochrome P450, putative
Locationchr10 : 40931855 .. 40932433 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAATTTAGTGAAAAACCCAGAAATCCAGAACAAGCTTTTGGCAGAGATGAAAGAAGTATTGGGAGATGGATTAAGGGAGGAGGTGAAGGAACAGGATCTGGGGAAACTTCCGTATCTGAAAGCCGTGGTTTTAGAAGGATTAAGAAGACACCCACCAGGGCATTTCCTACAACCACGAGGAGTGAAAGAGGATATACAATTTGATAATTACTTGATACCCAAAAACGGGACTGTGAATTTCTTGGCGGCGGAGATGGGTCGGGATCCAGAGGTGTGGGAAGATCCGATGGCGTTTAAGCCGGAGAGGTTCATGAAGGGCGGCGGAGGAGAAGAAGCGGCGGAGTTTGATGTAACAGGGAGCAAAGAGATAAAGATGATGCCATTCGGAGCAGGGAGGAGGATATGTCCTGGTTATGGTTTGGGGATTCTTCATTTGGAATATTTCTTGGCGAATTTGGTATGGAAATTTGAGTGGAAAGCTGTGGATGGTGAGAATGTTGATCTATCGGAGAAGATAGAGTTCACCTTTGTAATGAAAAAGCCTCTCAAAGCTTACATAGCTCCGAGGTTCTAA

mRNA sequence

ATGGCGAATTTAGTGAAAAACCCAGAAATCCAGAACAAGCTTTTGGCAGAGATGAAAGAAGTATTGGGAGATGGATTAAGGGAGGAGGTGAAGGAACAGGATCTGGGGAAACTTCCGTATCTGAAAGCCGTGGTTTTAGAAGGATTAAGAAGACACCCACCAGGGCATTTCCTACAACCACGAGGAGTGAAAGAGGATATACAATTTGATAATTACTTGATACCCAAAAACGGGACTGTGAATTTCTTGGCGGCGGAGATGGGTCGGGATCCAGAGGTGTGGGAAGATCCGATGGCGTTTAAGCCGGAGAGGTTCATGAAGGGCGGCGGAGGAGAAGAAGCGGCGGAGTTTGATGTAACAGGGAGCAAAGAGATAAAGATGATGCCATTCGGAGCAGGGAGGAGGATATGTCCTGGTTATGGTTTGGGGATTCTTCATTTGGAATATTTCTTGGCGAATTTGGTATGGAAATTTGAGTGGAAAGCTGTGGATGGTGAGAATGTTGATCTATCGGAGAAGATAGAGTTCACCTTTGTAATGAAAAAGCCTCTCAAAGCTTACATAGCTCCGAGGTTCTAA

Coding sequence (CDS)

ATGGCGAATTTAGTGAAAAACCCAGAAATCCAGAACAAGCTTTTGGCAGAGATGAAAGAAGTATTGGGAGATGGATTAAGGGAGGAGGTGAAGGAACAGGATCTGGGGAAACTTCCGTATCTGAAAGCCGTGGTTTTAGAAGGATTAAGAAGACACCCACCAGGGCATTTCCTACAACCACGAGGAGTGAAAGAGGATATACAATTTGATAATTACTTGATACCCAAAAACGGGACTGTGAATTTCTTGGCGGCGGAGATGGGTCGGGATCCAGAGGTGTGGGAAGATCCGATGGCGTTTAAGCCGGAGAGGTTCATGAAGGGCGGCGGAGGAGAAGAAGCGGCGGAGTTTGATGTAACAGGGAGCAAAGAGATAAAGATGATGCCATTCGGAGCAGGGAGGAGGATATGTCCTGGTTATGGTTTGGGGATTCTTCATTTGGAATATTTCTTGGCGAATTTGGTATGGAAATTTGAGTGGAAAGCTGTGGATGGTGAGAATGTTGATCTATCGGAGAAGATAGAGTTCACCTTTGTAATGAAAAAGCCTCTCAAAGCTTACATAGCTCCGAGGTTCTAA

Protein sequence

MANLVKNPEIQNKLLAEMKEVLGDGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFLQPRGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEEAAEFDVTGSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGENVDLSEKIEFTFVMKKPLKAYIAPRF
BLAST of Bhi10G001300 vs. TAIR10
Match: AT1G64940.1 (cytochrome P450, family 87, subfamily A, polypeptide 6)

HSP 1 Score: 258.5 bits (659), Expect = 3.4e-69
Identity = 123/191 (64.40%), Postives = 149/191 (78.01%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLGDGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFLQP 60
           MANLVKNPEIQ +L  E+K ++G+  + EV+EQD  K+PYLKAVV+EGLRRHPPGHF+ P
Sbjct: 326 MANLVKNPEIQRRLYEEIKSIVGEEAK-EVEEQDAQKMPYLKAVVMEGLRRHPPGHFVLP 385

Query: 61  RGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEEAAEFDVT 120
             V ED     Y +PK GT+NF+ AE+GRDP+VWE+PMAFKPERFM     EEA   D+T
Sbjct: 386 HSVTEDTVLGGYKVPKKGTINFMVAEIGRDPKVWEEPMAFKPERFM-----EEAV--DIT 445

Query: 121 GSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGENVDLSEKIEFTFVM 180
           GS+ IKMMPFGAGRRICPG GL +LHLEY++AN+V +FEW+ V G  VDL+EK+EFT VM
Sbjct: 446 GSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREFEWQEVQGHEVDLTEKLEFTVVM 505

Query: 181 KKPLKAYIAPR 192
           K PLKA   PR
Sbjct: 506 KHPLKALAVPR 508

BLAST of Bhi10G001300 vs. TAIR10
Match: AT3G03470.1 (cytochrome P450, family 87, subfamily A, polypeptide 9)

HSP 1 Score: 257.7 bits (657), Expect = 5.7e-69
Identity = 122/194 (62.89%), Postives = 149/194 (76.80%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLG--DGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFL 60
           MA +VK PEIQ K+  EMK V    +  REE++E+DLGKL YLKAV+LE LRRHPPGH+L
Sbjct: 319 MAIMVKYPEIQRKVYEEMKTVFAGEEEEREEIREEDLGKLSYLKAVILECLRRHPPGHYL 378

Query: 61  QPRGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEEAAEFD 120
               V  D     +LIP+ GT+NF+  EMGRDP++WEDP+ FKPERF++ G   EA +FD
Sbjct: 379 SYHKVTHDTVLGGFLIPRQGTINFMVGEMGRDPKIWEDPLTFKPERFLENG---EACDFD 438

Query: 121 VTGSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGENVDLSEKIEF-T 180
           +TG++EIKMMPFGAGRR+CPGY L +LHLEY++ANLVWKFEWK V+GE VDLSEK +F T
Sbjct: 439 MTGTREIKMMPFGAGRRMCPGYALSLLHLEYYVANLVWKFEWKCVEGEEVDLSEKQQFIT 498

Query: 181 FVMKKPLKAYIAPR 192
            VMK P KA I PR
Sbjct: 499 MVMKNPFKANIYPR 509

BLAST of Bhi10G001300 vs. TAIR10
Match: AT1G64900.1 (cytochrome P450, family 89, subfamily A, polypeptide 2)

HSP 1 Score: 257.3 bits (656), Expect = 7.5e-69
Identity = 125/191 (65.45%), Postives = 149/191 (78.01%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLGDGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFLQP 60
           MANLVK PEIQ +L  E+K V+G+  + EV+E+D+ K+PYLKAVVLEGLRRHPPGHFL P
Sbjct: 319 MANLVKYPEIQERLHEEIKSVVGEEAK-EVEEEDVEKMPYLKAVVLEGLRRHPPGHFLLP 378

Query: 61  RGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEEAAEFDVT 120
             V ED     Y +PKNGT+NF+ AE+GRDP  WE+PMAFKPERFM    GEE A  D+T
Sbjct: 379 HSVTEDTVLGGYKVPKNGTINFMVAEIGRDPVEWEEPMAFKPERFM----GEEEA-VDLT 438

Query: 121 GSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGENVDLSEKIEFTFVM 180
           GS+ IKMMPFGAGRRICPG GL +LHLEY++AN+V +F+WK V G  VDL+EK+EFT VM
Sbjct: 439 GSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREFQWKEVQGHEVDLTEKLEFTVVM 498

Query: 181 KKPLKAYIAPR 192
           K PLKA   PR
Sbjct: 499 KHPLKALAVPR 503

BLAST of Bhi10G001300 vs. TAIR10
Match: AT1G64950.1 (cytochrome P450, family 89, subfamily A, polypeptide 5)

HSP 1 Score: 256.9 bits (655), Expect = 9.8e-69
Identity = 122/191 (63.87%), Postives = 149/191 (78.01%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLGDGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFLQP 60
           MANLVKNP+IQ +L  E+K V+G+    EV+E+D  K+PYL+AVV+EGLRRHPPGHF+ P
Sbjct: 325 MANLVKNPDIQKRLYEEIKSVVGEE-ANEVEEEDAQKMPYLEAVVMEGLRRHPPGHFVLP 384

Query: 61  RGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEEAAEFDVT 120
             V ED     Y +PKNGT+NF+ AE+GRDP+VWE+PMAFKPERFM     EEA   D+T
Sbjct: 385 HSVTEDTVLGGYKVPKNGTINFMVAEIGRDPKVWEEPMAFKPERFM-----EEAV--DIT 444

Query: 121 GSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGENVDLSEKIEFTFVM 180
           GS+ IKMMPFGAGRRICPG GL +LHLEY++AN+V +F+WK V G  VDL+EK+EFT VM
Sbjct: 445 GSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREFDWKEVQGHEVDLTEKLEFTVVM 504

Query: 181 KKPLKAYIAPR 192
           K PLKA   PR
Sbjct: 505 KHPLKALAVPR 507

BLAST of Bhi10G001300 vs. TAIR10
Match: AT1G64930.1 (cytochrome P450, family 87, subfamily A, polypeptide 7)

HSP 1 Score: 245.4 bits (625), Expect = 2.9e-65
Identity = 118/191 (61.78%), Postives = 146/191 (76.44%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLGDGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFLQP 60
           MANLVKN EIQ +L  E+  V+G+  +  V+E+D  K+PYLKAVV+E LRRHPPG+ + P
Sbjct: 324 MANLVKNQEIQERLYEEITNVVGEEAK-VVEEKDTQKMPYLKAVVMEALRRHPPGNTVLP 383

Query: 61  RGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEEAAEFDVT 120
             V ED     Y +PK GT+NFL AE+GRDP+VWE+PMAFKPERFM    GEE A  D+T
Sbjct: 384 HSVTEDTVLGGYKVPKKGTINFLVAEIGRDPKVWEEPMAFKPERFM----GEEEA-VDIT 443

Query: 121 GSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGENVDLSEKIEFTFVM 180
           GS+ IKMMPFGAGRRICPG GL +LHLEY++AN+V +F+WK V+G  VDL+EK+EFT +M
Sbjct: 444 GSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREFQWKEVEGHEVDLTEKVEFTVIM 503

Query: 181 KKPLKAYIAPR 192
           K PLKA   PR
Sbjct: 504 KHPLKAIAVPR 508

BLAST of Bhi10G001300 vs. Swiss-Prot
Match: sp|Q9SRQ1|C89A9_ARATH (Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.0e-67
Identity = 122/194 (62.89%), Postives = 149/194 (76.80%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLG--DGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFL 60
           MA +VK PEIQ K+  EMK V    +  REE++E+DLGKL YLKAV+LE LRRHPPGH+L
Sbjct: 319 MAIMVKYPEIQRKVYEEMKTVFAGEEEEREEIREEDLGKLSYLKAVILECLRRHPPGHYL 378

Query: 61  QPRGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEEAAEFD 120
               V  D     +LIP+ GT+NF+  EMGRDP++WEDP+ FKPERF++ G   EA +FD
Sbjct: 379 SYHKVTHDTVLGGFLIPRQGTINFMVGEMGRDPKIWEDPLTFKPERFLENG---EACDFD 438

Query: 121 VTGSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGENVDLSEKIEF-T 180
           +TG++EIKMMPFGAGRR+CPGY L +LHLEY++ANLVWKFEWK V+GE VDLSEK +F T
Sbjct: 439 MTGTREIKMMPFGAGRRMCPGYALSLLHLEYYVANLVWKFEWKCVEGEEVDLSEKQQFIT 498

Query: 181 FVMKKPLKAYIAPR 192
            VMK P KA I PR
Sbjct: 499 MVMKNPFKANIYPR 509

BLAST of Bhi10G001300 vs. Swiss-Prot
Match: sp|Q42602|C89A2_ARATH (Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2)

HSP 1 Score: 257.3 bits (656), Expect = 1.3e-67
Identity = 125/191 (65.45%), Postives = 149/191 (78.01%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLGDGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFLQP 60
           MANLVK PEIQ +L  E+K V+G+  + EV+E+D+ K+PYLKAVVLEGLRRHPPGHFL P
Sbjct: 319 MANLVKYPEIQERLHEEIKSVVGEEAK-EVEEEDVEKMPYLKAVVLEGLRRHPPGHFLLP 378

Query: 61  RGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEEAAEFDVT 120
             V ED     Y +PKNGT+NF+ AE+GRDP  WE+PMAFKPERFM    GEE A  D+T
Sbjct: 379 HSVTEDTVLGGYKVPKNGTINFMVAEIGRDPVEWEEPMAFKPERFM----GEEEA-VDLT 438

Query: 121 GSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGENVDLSEKIEFTFVM 180
           GS+ IKMMPFGAGRRICPG GL +LHLEY++AN+V +F+WK V G  VDL+EK+EFT VM
Sbjct: 439 GSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREFQWKEVQGHEVDLTEKLEFTVVM 498

Query: 181 KKPLKAYIAPR 192
           K PLKA   PR
Sbjct: 499 KHPLKALAVPR 503

BLAST of Bhi10G001300 vs. Swiss-Prot
Match: sp|P37123|C77A1_SOLME (Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 7.2e-45
Identity = 90/192 (46.88%), Postives = 120/192 (62.50%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLGDGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFLQP 60
           +  L++NP IQN+L  E+K ++GD   ++V E D+ K+PYL AVV E LR+HPP +F   
Sbjct: 315 IGRLMENPTIQNQLYQEIKTIVGD---KKVDENDIEKMPYLNAVVKELLRKHPPTYFTLT 374

Query: 61  RGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEEAAEFDVT 120
             V E ++   Y IP +  V F    +  DP VW DP  F P+RF+ G       + D+T
Sbjct: 375 HSVTEPVKLAGYDIPMDTNVEFFVHGISHDPNVWSDPEKFDPDRFLSG-----REDADIT 434

Query: 121 GSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGEN-VDLSEKIEFTFV 180
           G KE+KMMPFG GRRICPG G+  +H+   LA +V +FEW A  G N VD SEK+EFT V
Sbjct: 435 GVKEVKMMPFGVGRRICPGLGMATVHVNLMLARMVQEFEWFAYPGNNKVDFSEKLEFTVV 494

Query: 181 MKKPLKAYIAPR 192
           MK PL+A +  R
Sbjct: 495 MKNPLRAKVKLR 498

BLAST of Bhi10G001300 vs. Swiss-Prot
Match: sp|O48928|C77A3_SOYBN (Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 7.7e-39
Identity = 85/192 (44.27%), Postives = 115/192 (59.90%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLGDGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFLQP 60
           +A L+ NP +Q KL  E+K  +G+   ++V E+D+ K+PYL AVV E LR+HPP HF+  
Sbjct: 323 IAQLIANPNVQTKLYEEIKRTVGE---KKVDEKDVEKMPYLHAVVKELLRKHPPTHFVLT 382

Query: 61  RGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEEAAEFDVT 120
             V E      Y IP +  V      +  DP+ W +P  F PERF+   GGEEA   D+T
Sbjct: 383 HAVTEPTTLGGYDIPIDANVEVYTPAIAEDPKNWLNPEKFDPERFI--SGGEEA---DIT 442

Query: 121 GSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGE-NVDLSEKIEFTFV 180
           G   +KMMPFG GRRICPG  +  +H+   +A +V +FEW A   E  +D + K EFT V
Sbjct: 443 GVTGVKMMPFGVGRRICPGLAMATVHIHLMMARMVQEFEWGAYPPEKKMDFTGKWEFTVV 502

Query: 181 MKKPLKAYIAPR 192
           MK+ L+A I PR
Sbjct: 503 MKESLRATIKPR 506

BLAST of Bhi10G001300 vs. Swiss-Prot
Match: sp|Q9LZ31|C77A4_ARATH (Cytochrome P450 77A4 OS=Arabidopsis thaliana OX=3702 GN=CYP77A4 PE=2 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 6.5e-38
Identity = 80/192 (41.67%), Postives = 111/192 (57.81%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLGDGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFLQP 60
           +A L+ NPEIQ++L  E+K  +GD  R  V E+D+ K+ +L+A V E LR+HPP +F   
Sbjct: 327 IAQLIANPEIQSRLYDEIKSTVGDDRR--VDEKDVDKMVFLQAFVKELLRKHPPTYFSLT 386

Query: 61  RGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEEAAEFDVT 120
             V E      Y IP    V      +  DP +W +P  F P+RFM G       + D+T
Sbjct: 387 HAVMETTTLAGYDIPAGVNVEVYLPGISEDPRIWNNPKKFDPDRFMLG-----KEDADIT 446

Query: 121 GSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKA-VDGENVDLSEKIEFTFV 180
           G   +KM+PFG GRRICPG  +  +H+   LA +V +FEW A   G  +D + K+EFT V
Sbjct: 447 GISGVKMIPFGVGRRICPGLAMATIHVHLMLARMVQEFEWCAHPPGSEIDFAGKLEFTVV 506

Query: 181 MKKPLKAYIAPR 192
           MK PL+A + PR
Sbjct: 507 MKNPLRAMVKPR 511

BLAST of Bhi10G001300 vs. TrEMBL
Match: tr|A0A0A0LNK3|A0A0A0LNK3_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G437040 PE=3 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 4.8e-94
Identity = 166/191 (86.91%), Postives = 178/191 (93.19%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLGDGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFLQP 60
           MANLVKNPEIQ+KL  EMKEV+GDG REEVKE+DLGKLPYLKAVVLEGLRRHPPGHFLQ 
Sbjct: 363 MANLVKNPEIQHKLFTEMKEVMGDGTREEVKEEDLGKLPYLKAVVLEGLRRHPPGHFLQA 422

Query: 61  RGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEEAAEFDVT 120
           RGVKEDIQF+NYLIPKNGTVNFLAAE+GRDP VWE+PMAFKPERFM G GGEEAA FDVT
Sbjct: 423 RGVKEDIQFENYLIPKNGTVNFLAAEIGRDPTVWEEPMAFKPERFMNGDGGEEAAGFDVT 482

Query: 121 GSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGENVDLSEKIEFTFVM 180
           GSKEIKMMPFGAGRRICPGYGLGILHLEYFLANL+WKF+W+ V+G+NVDLSEK+EFT VM
Sbjct: 483 GSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLLWKFDWRGVEGDNVDLSEKLEFTVVM 542

Query: 181 KKPLKAYIAPR 192
           KKPLKA I  R
Sbjct: 543 KKPLKANIILR 553

BLAST of Bhi10G001300 vs. TrEMBL
Match: tr|A0A1S3CAR2|A0A1S3CAR2_CUCME (cytochrome P450 89A2-like OS=Cucumis melo OX=3656 GN=LOC103498359 PE=3 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 6.2e-86
Identity = 151/193 (78.24%), Postives = 171/193 (88.60%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLGDGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFLQP 60
           MANLVKNPEIQNKL AE+K V+GDG REEVKE++LGKLPYLKAVVLEGLRRHPPGHF+ P
Sbjct: 339 MANLVKNPEIQNKLYAEIKGVMGDGSREEVKEEELGKLPYLKAVVLEGLRRHPPGHFVLP 398

Query: 61  RGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEE-AAEFDV 120
             VKED + +NY+IPKNG+VNF+ AEMG DP+VWEDPM FKPERFMKGG GEE   EFD+
Sbjct: 399 HAVKEDTELENYVIPKNGSVNFMVAEMGWDPKVWEDPMEFKPERFMKGGAGEEGGVEFDI 458

Query: 121 TGSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGENVDLSEKIEFTFV 180
           TGSKEIKMMPFGAGRRICPG+GL ILHLEYF+ NLVWKFEWK V+GE+VDL+EK+EFT V
Sbjct: 459 TGSKEIKMMPFGAGRRICPGFGLAILHLEYFIGNLVWKFEWKGVEGEDVDLTEKVEFTVV 518

Query: 181 MKKPLKAYIAPRF 193
           M+KPLKA +  RF
Sbjct: 519 MEKPLKANVICRF 531

BLAST of Bhi10G001300 vs. TrEMBL
Match: tr|A0A0A0LTQ9|A0A0A0LTQ9_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G435540 PE=3 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 2.4e-85
Identity = 150/192 (78.12%), Postives = 168/192 (87.50%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLGDGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFLQP 60
           MANLVK PEIQNKLL EMK V+GDG  EEVKE+DLGKLPYLKAV+LEGLRRHPP HFL P
Sbjct: 321 MANLVKYPEIQNKLLVEMKGVMGDGSEEEVKEEDLGKLPYLKAVILEGLRRHPPAHFLLP 380

Query: 61  RGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEEAAEFDVT 120
             VKE+ +  NY+IPKNGT N++ AEMGRDP+VWEDPMAFKPERFMK  GGEE  EFD+T
Sbjct: 381 HAVKEETKLGNYVIPKNGTTNYMVAEMGRDPKVWEDPMAFKPERFMK--GGEEGIEFDIT 440

Query: 121 GSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGENVDLSEKIEFTFVM 180
           GSKEIKMMPFGAGRR+CPG+GL ILHLEYF+ANLVW+FEWKAVDG+ VDLSEK+E T VM
Sbjct: 441 GSKEIKMMPFGAGRRMCPGFGLAILHLEYFVANLVWRFEWKAVDGDEVDLSEKVELTIVM 500

Query: 181 KKPLKAYIAPRF 193
           KKPL+A + PRF
Sbjct: 501 KKPLQANLRPRF 510

BLAST of Bhi10G001300 vs. TrEMBL
Match: tr|A0A0A0LNB6|A0A0A0LNB6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G437050 PE=3 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.2e-84
Identity = 149/193 (77.20%), Postives = 169/193 (87.56%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLGDGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFLQP 60
           MANLVKNPEIQNKL AE+K V+GDG REEV E++LGKLPYLKAVVLEGLRRHPPGHF+ P
Sbjct: 268 MANLVKNPEIQNKLYAEIKGVMGDGSREEVMEEELGKLPYLKAVVLEGLRRHPPGHFVLP 327

Query: 61  RGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEE-AAEFDV 120
             VKED + +NY+IPKNG+VNF+ AEMG DP+VWEDPM FKPERFMKGG GEE   EFD+
Sbjct: 328 HAVKEDTELENYVIPKNGSVNFMVAEMGWDPKVWEDPMEFKPERFMKGGAGEEGVVEFDI 387

Query: 121 TGSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGENVDLSEKIEFTFV 180
           TGSKE KMMPFGAGRR+CPG+GL ILHLEYF+ NLVWKFEWK V+GE+VDLSEK+EFT V
Sbjct: 388 TGSKERKMMPFGAGRRVCPGFGLAILHLEYFIGNLVWKFEWKGVEGEDVDLSEKVEFTVV 447

Query: 181 MKKPLKAYIAPRF 193
           M+KPLKA +  RF
Sbjct: 448 MEKPLKANVICRF 460

BLAST of Bhi10G001300 vs. TrEMBL
Match: tr|A0A0A0LQX4|A0A0A0LQX4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G435520 PE=3 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 3.2e-82
Identity = 148/191 (77.49%), Postives = 164/191 (85.86%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLGDGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFLQP 60
           MANLVKNPEIQNKLL+EMK V+GDG REEVKE+DL KLPYLKA++LEGLRRHPPGHFL P
Sbjct: 323 MANLVKNPEIQNKLLSEMKGVMGDGSREEVKEEDLEKLPYLKAMILEGLRRHPPGHFLLP 382

Query: 61  RGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEEAAEFDVT 120
             VKED   +NY+IPKNG VNF+ AE+G DP+VWEDPMAFKPERFMKG  G E  EFD+T
Sbjct: 383 HAVKEDTILENYVIPKNGIVNFMVAEIGWDPKVWEDPMAFKPERFMKGNEG-EGVEFDIT 442

Query: 121 GSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGENVDLSEKIEFTFVM 180
           GSKEIKMMPFGAGRR+CPG GL ILHLEYF+ANLVW+FEWK V GE V L EK+EFT VM
Sbjct: 443 GSKEIKMMPFGAGRRMCPGLGLAILHLEYFIANLVWRFEWKEVKGEEVSLLEKVEFTVVM 502

Query: 181 KKPLKAYIAPR 192
           +KPLKA I PR
Sbjct: 503 EKPLKANIIPR 512

BLAST of Bhi10G001300 vs. NCBI nr
Match: XP_011650075.1 (PREDICTED: cytochrome P450 89A2-like [Cucumis sativus] >KGN63393.1 hypothetical protein Csa_2G437040 [Cucumis sativus])

HSP 1 Score: 352.8 bits (904), Expect = 7.2e-94
Identity = 166/191 (86.91%), Postives = 178/191 (93.19%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLGDGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFLQP 60
           MANLVKNPEIQ+KL  EMKEV+GDG REEVKE+DLGKLPYLKAVVLEGLRRHPPGHFLQ 
Sbjct: 363 MANLVKNPEIQHKLFTEMKEVMGDGTREEVKEEDLGKLPYLKAVVLEGLRRHPPGHFLQA 422

Query: 61  RGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEEAAEFDVT 120
           RGVKEDIQF+NYLIPKNGTVNFLAAE+GRDP VWE+PMAFKPERFM G GGEEAA FDVT
Sbjct: 423 RGVKEDIQFENYLIPKNGTVNFLAAEIGRDPTVWEEPMAFKPERFMNGDGGEEAAGFDVT 482

Query: 121 GSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGENVDLSEKIEFTFVM 180
           GSKEIKMMPFGAGRRICPGYGLGILHLEYFLANL+WKF+W+ V+G+NVDLSEK+EFT VM
Sbjct: 483 GSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLLWKFDWRGVEGDNVDLSEKLEFTVVM 542

Query: 181 KKPLKAYIAPR 192
           KKPLKA I  R
Sbjct: 543 KKPLKANIILR 553

BLAST of Bhi10G001300 vs. NCBI nr
Match: XP_022944210.1 (cytochrome P450 89A2-like [Cucurbita moschata])

HSP 1 Score: 327.4 bits (838), Expect = 3.2e-86
Identity = 152/191 (79.58%), Postives = 169/191 (88.48%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLGDGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFLQP 60
           MANLVK PEIQ+KL AEMK V+GDG  EEVKE+DLGKLPYLKAVVLEGLRRHPPGHF+ P
Sbjct: 329 MANLVKYPEIQHKLFAEMKGVMGDGTGEEVKEEDLGKLPYLKAVVLEGLRRHPPGHFVLP 388

Query: 61  RGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEEAAEFDVT 120
             VK+D   +NY+IPKNGTVNF+ AEMG DPEVWEDPMAFKPERFMKGG  EE A FD+T
Sbjct: 389 HAVKDDTMLENYVIPKNGTVNFMVAEMGWDPEVWEDPMAFKPERFMKGGEEEEVAGFDIT 448

Query: 121 GSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGENVDLSEKIEFTFVM 180
           GSKEIKMMPFGAGRR+CPG+GL ILHLEYF+ANLVW+FEWKAV+G+ VDLSEK+EFT VM
Sbjct: 449 GSKEIKMMPFGAGRRMCPGFGLAILHLEYFIANLVWRFEWKAVEGDGVDLSEKVEFTVVM 508

Query: 181 KKPLKAYIAPR 192
           +KPLKA I PR
Sbjct: 509 EKPLKANIIPR 519

BLAST of Bhi10G001300 vs. NCBI nr
Match: XP_008459160.1 (PREDICTED: cytochrome P450 89A2-like [Cucumis melo])

HSP 1 Score: 325.9 bits (834), Expect = 9.4e-86
Identity = 151/193 (78.24%), Postives = 171/193 (88.60%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLGDGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFLQP 60
           MANLVKNPEIQNKL AE+K V+GDG REEVKE++LGKLPYLKAVVLEGLRRHPPGHF+ P
Sbjct: 339 MANLVKNPEIQNKLYAEIKGVMGDGSREEVKEEELGKLPYLKAVVLEGLRRHPPGHFVLP 398

Query: 61  RGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEE-AAEFDV 120
             VKED + +NY+IPKNG+VNF+ AEMG DP+VWEDPM FKPERFMKGG GEE   EFD+
Sbjct: 399 HAVKEDTELENYVIPKNGSVNFMVAEMGWDPKVWEDPMEFKPERFMKGGAGEEGGVEFDI 458

Query: 121 TGSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGENVDLSEKIEFTFV 180
           TGSKEIKMMPFGAGRRICPG+GL ILHLEYF+ NLVWKFEWK V+GE+VDL+EK+EFT V
Sbjct: 459 TGSKEIKMMPFGAGRRICPGFGLAILHLEYFIGNLVWKFEWKGVEGEDVDLTEKVEFTVV 518

Query: 181 MKKPLKAYIAPRF 193
           M+KPLKA +  RF
Sbjct: 519 MEKPLKANVICRF 531

BLAST of Bhi10G001300 vs. NCBI nr
Match: XP_023512645.1 (cytochrome P450 89A2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 324.3 bits (830), Expect = 2.7e-85
Identity = 152/192 (79.17%), Postives = 172/192 (89.58%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLGDGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFLQP 60
           MANLVK PEIQ+KL AEMK V+GDG  EEVKE+DLGKLPYLKAVVLEGLRRHPPG FL+ 
Sbjct: 329 MANLVKYPEIQHKLFAEMKGVMGDGTSEEVKEEDLGKLPYLKAVVLEGLRRHPPGLFLEA 388

Query: 61  RGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEEAAEFDVT 120
           RGVKEDI+F+NY+IPKN TVNF+A EMGR  +VWEDPMAFKPERFM GG    AA FDVT
Sbjct: 389 RGVKEDIEFENYVIPKNATVNFMATEMGRGADVWEDPMAFKPERFMGGGA---AAAFDVT 448

Query: 121 GSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGENVDLSEKIEFTFVM 180
           GS+EIKMMPFGAGRR+CPGYGLGILHLEY+LAN++W+FEWK+VDG++VDLSEK+EFT VM
Sbjct: 449 GSREIKMMPFGAGRRMCPGYGLGILHLEYYLANMIWRFEWKSVDGDDVDLSEKVEFTVVM 508

Query: 181 KKPLKAYIAPRF 193
           K PLKA I+PRF
Sbjct: 509 KNPLKAIISPRF 517

BLAST of Bhi10G001300 vs. NCBI nr
Match: XP_011650074.1 (PREDICTED: cytochrome P450 89A2-like [Cucumis sativus] >KGN63391.1 hypothetical protein Csa_2G435540 [Cucumis sativus])

HSP 1 Score: 323.9 bits (829), Expect = 3.6e-85
Identity = 150/192 (78.12%), Postives = 168/192 (87.50%), Query Frame = 0

Query: 1   MANLVKNPEIQNKLLAEMKEVLGDGLREEVKEQDLGKLPYLKAVVLEGLRRHPPGHFLQP 60
           MANLVK PEIQNKLL EMK V+GDG  EEVKE+DLGKLPYLKAV+LEGLRRHPP HFL P
Sbjct: 321 MANLVKYPEIQNKLLVEMKGVMGDGSEEEVKEEDLGKLPYLKAVILEGLRRHPPAHFLLP 380

Query: 61  RGVKEDIQFDNYLIPKNGTVNFLAAEMGRDPEVWEDPMAFKPERFMKGGGGEEAAEFDVT 120
             VKE+ +  NY+IPKNGT N++ AEMGRDP+VWEDPMAFKPERFMK  GGEE  EFD+T
Sbjct: 381 HAVKEETKLGNYVIPKNGTTNYMVAEMGRDPKVWEDPMAFKPERFMK--GGEEGIEFDIT 440

Query: 121 GSKEIKMMPFGAGRRICPGYGLGILHLEYFLANLVWKFEWKAVDGENVDLSEKIEFTFVM 180
           GSKEIKMMPFGAGRR+CPG+GL ILHLEYF+ANLVW+FEWKAVDG+ VDLSEK+E T VM
Sbjct: 441 GSKEIKMMPFGAGRRMCPGFGLAILHLEYFVANLVWRFEWKAVDGDEVDLSEKVELTIVM 500

Query: 181 KKPLKAYIAPRF 193
           KKPL+A + PRF
Sbjct: 501 KKPLQANLRPRF 510

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G64940.13.4e-6964.40cytochrome P450, family 87, subfamily A, polypeptide 6[more]
AT3G03470.15.7e-6962.89cytochrome P450, family 87, subfamily A, polypeptide 9[more]
AT1G64900.17.5e-6965.45cytochrome P450, family 89, subfamily A, polypeptide 2[more]
AT1G64950.19.8e-6963.87cytochrome P450, family 89, subfamily A, polypeptide 5[more]
AT1G64930.12.9e-6561.78cytochrome P450, family 87, subfamily A, polypeptide 7[more]
Match NameE-valueIdentityDescription
sp|Q9SRQ1|C89A9_ARATH1.0e-6762.89Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1[more]
sp|Q42602|C89A2_ARATH1.3e-6765.45Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2[more]
sp|P37123|C77A1_SOLME7.2e-4546.88Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=... [more]
sp|O48928|C77A3_SOYBN7.7e-3944.27Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1[more]
sp|Q9LZ31|C77A4_ARATH6.5e-3841.67Cytochrome P450 77A4 OS=Arabidopsis thaliana OX=3702 GN=CYP77A4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A0A0LNK3|A0A0A0LNK3_CUCSA4.8e-9486.91Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G437040 PE=3 SV=1[more]
tr|A0A1S3CAR2|A0A1S3CAR2_CUCME6.2e-8678.24cytochrome P450 89A2-like OS=Cucumis melo OX=3656 GN=LOC103498359 PE=3 SV=1[more]
tr|A0A0A0LTQ9|A0A0A0LTQ9_CUCSA2.4e-8578.13Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G435540 PE=3 SV=1[more]
tr|A0A0A0LNB6|A0A0A0LNB6_CUCSA1.2e-8477.20Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G437050 PE=3 SV=1[more]
tr|A0A0A0LQX4|A0A0A0LQX4_CUCSA3.2e-8277.49Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G435520 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_011650075.17.2e-9486.91PREDICTED: cytochrome P450 89A2-like [Cucumis sativus] >KGN63393.1 hypothetical ... [more]
XP_022944210.13.2e-8679.58cytochrome P450 89A2-like [Cucurbita moschata][more]
XP_008459160.19.4e-8678.24PREDICTED: cytochrome P450 89A2-like [Cucumis melo][more]
XP_023512645.12.7e-8579.17cytochrome P450 89A2-like [Cucurbita pepo subsp. pepo][more]
XP_011650074.13.6e-8578.13PREDICTED: cytochrome P450 89A2-like [Cucumis sativus] >KGN63391.1 hypothetical ... [more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0005506iron ion binding
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0004497monooxygenase activity
GO:0020037heme binding
Vocabulary: INTERPRO
TermDefinition
IPR017972Cyt_P450_CS
IPR036396Cyt_P450_sf
IPR001128Cyt_P450
IPR002403Cyt_P450_E_grp-IV
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi10M001300Bhi10M001300mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 88..106
score: 35.22
coord: 38..54
score: 38.91
coord: 121..137
score: 37.56
coord: 137..155
score: 24.09
IPR001128Cytochrome P450PRINTSPR00385P450coord: 6..19
score: 35.83
coord: 128..137
score: 60.88
coord: 43..54
score: 40.67
coord: 137..148
score: 30.19
IPR001128Cytochrome P450PFAMPF00067p450coord: 2..183
e-value: 1.4E-48
score: 165.7
IPR036396Cytochrome P450 superfamilyGENE3DG3DSA:1.10.630.10coord: 1..189
e-value: 1.1E-56
score: 194.5
IPR036396Cytochrome P450 superfamilySUPERFAMILYSSF48264Cytochrome P450coord: 1..191
NoneNo IPR availablePANTHERPTHR24298FAMILY NOT NAMEDcoord: 1..188
NoneNo IPR availablePANTHERPTHR24298:SF45CYTOCHROME P450 89A2-RELATEDcoord: 1..188
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 130..139