Bhi10G001254 (gene) Wax gourd

BLAST of Bhi10G001254 vs. TAIR10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5)

HSP 1 Score: 325.1 bits (832), Expect = 4.8e-89
Identity = 159/309 (51.46%), Postives = 213/309 (68.93%), Query Frame = 0

Query: 1   MVCGKKFY-----EEDYSNKFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRVKKLA 60
           MV GK++Y     + D +   REL+ +IMA  G+ N  D++P  NW+  T F  + K L 
Sbjct: 189 MVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--TNFENQTKILG 248

Query: 61  KTSDEFLQQLIDEIRNQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGIDTTA 120
              D  LQ+L+DE R + + G T+I HLLS Q  EP+YY+D  IKG+I  ++LAG DT++
Sbjct: 249 NRLDRVLQKLVDEKRAEKEKGQTLIDHLLSFQETEPEYYTDVIIKGIILALVLAGTDTSS 308

Query: 121 VTLEWTLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLNPPVP 180
           VTLEW +S LLN+PE+LEKA+ EID  IG +RLV E+++ +L YLQ I+SE+LRL P VP
Sbjct: 309 VTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETLRLYPAVP 368

Query: 181 LLVPHCALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEVDYSY 240
           LL+PH +  +ECK+ GYD+PR T++L NVWA+HRD  +WE+   FKPER     E   + 
Sbjct: 369 LLLPHFS-SDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERFEKEGE---AR 428

Query: 241 KLIPFGSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEGKGLTMPKTRPLV 300
           KL+PFG GRRACPG  + +R+V L L  LIQSFEW+RV   L+DM EG+G+TMPK  PL 
Sbjct: 429 KLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGITMPKATPLR 488

Query: 301 AKCKPRPIM 305
           A CK R I+
Sbjct: 489 AMCKARAIV 491

BLAST of Bhi10G001254 vs. TAIR10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3)

HSP 1 Score: 322.4 bits (825), Expect = 3.1e-88
Identity = 166/316 (52.53%), Postives = 216/316 (68.35%), Query Frame = 0

Query: 1   MVCGKKFY-----EEDYSNKFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRVKKLA 60
           M+ GK +Y     ++  + + REL+ + M   GA N  D++P+  WI  TG  KR+KK+A
Sbjct: 188 MLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI--TGSEKRIKKIA 247

Query: 61  KTSDEFLQQLIDEIR-NQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGIDTT 120
              DEFLQ L+DE R  +    NTM+ HLL LQ  +P+YY+D  IKG++  ++LAG DT+
Sbjct: 248 SRLDEFLQGLVDERREGKEKRQNTMVDHLLCLQETQPEYYTDNIIKGIMLSLILAGTDTS 307

Query: 121 AVTLEWTLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLNPPV 180
           AVTLEWTLS LLN+P++L KA+ EID+ +G  RLV E++L  L YLQ I+SESLRL P  
Sbjct: 308 AVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLRLYPAS 367

Query: 181 PLLVPHCALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEVDYS 240
           PLLVPH A  E+CK+ GY +PR T++L N WAIHRD  IW+D TSFKPER     E   +
Sbjct: 368 PLLVPHVA-SEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERFEKEGE---A 427

Query: 241 YKLIPFGSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNE-GKGLTMPKTRP 300
            KL+ FG GRRACPG G+AQR+  LT+ SLIQ FEW+R+    +DM E G G+ MPK  P
Sbjct: 428 QKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGGGVIMPKAIP 487

Query: 301 LVAKCKPRPIMKIILN 310
           LVA CK RP++  ILN
Sbjct: 488 LVAMCKARPVVGKILN 497

BLAST of Bhi10G001254 vs. TAIR10
Match: AT1G66540.1 (Cytochrome P450 superfamily protein)

HSP 1 Score: 320.1 bits (819), Expect = 1.5e-87
Identity = 161/307 (52.44%), Postives = 217/307 (70.68%), Query Frame = 0

Query: 1   MVCGKKFY-----EEDYSNKFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRVKKLA 60
           MV GK +Y     ++  + + R+L+ + M+  GA +  D +P+  WI  T F +RVKK+A
Sbjct: 75  MVTGKCYYGDGAEDDPEAKRVRQLIAEAMSCFGAGHAADHLPMLRWI--TDFERRVKKIA 134

Query: 61  KTSDEFLQQLIDEIR-NQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGIDTT 120
              DEF Q+L+DE R  +    NTMI HLLSLQ  +P+YY+D TIKG +  ++LAG DT+
Sbjct: 135 ARLDEFFQRLVDEKRVAKEKKENTMIDHLLSLQVSQPEYYTDHTIKGTMLSLILAGTDTS 194

Query: 121 AVTLEWTLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLNPPV 180
           AVTLEW LS LLNNPEVL+K + EID+ IG +RL+ E+++P+L YLQ I+SE+LRL P  
Sbjct: 195 AVTLEWALSSLLNNPEVLKKVRDEIDNQIGLDRLLEESDIPNLPYLQNIVSETLRLYPAG 254

Query: 181 PLLVPHCALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEVDYS 240
           PLLVPH +  E+CK+ GYD+P  T++L+NVWAIHRD  +W+D  SFKPER     E   +
Sbjct: 255 PLLVPHIS-SEDCKVGGYDMPCGTMLLVNVWAIHRDPRLWDDPASFKPERFEKEGE---T 314

Query: 241 YKLIPFGSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEGKGLTMPKTRPL 300
           +KL+ FG GRRACPG G+A+R+V L+L SLIQ FEW+R+    +DM EG GLTMP+  PL
Sbjct: 315 HKLLTFGLGRRACPGSGLARRLVSLSLGSLIQCFEWERIGEEEVDMTEGGGLTMPRAIPL 374

Query: 301 VAKCKPR 302
           VA C+ R
Sbjct: 375 VAMCRAR 375

BLAST of Bhi10G001254 vs. TAIR10
Match: AT3G28740.1 (Cytochrome P450 superfamily protein)

HSP 1 Score: 319.3 bits (817), Expect = 2.6e-87
Identity = 165/316 (52.22%), Postives = 211/316 (66.77%), Query Frame = 0

Query: 1   MVCGKKFYEEDYSN-----KFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRVKKLA 60
           MV GK+FY +   N       R+L+ +++  GGA N  D+ P+  ++  T + K VKKLA
Sbjct: 197 MVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TNYEKHVKKLA 256

Query: 61  KTSDEFLQQLIDEIRNQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGIDTTA 120
              DEFLQ L++E R +   GNTMI HLLSLQ  +P YY+D  IKG+I  ++LAG DT+A
Sbjct: 257 GRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKGIILVMILAGTDTSA 316

Query: 121 VTLEWTLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLNPPVP 180
            TLEW +S LLN+PEVL KAK EID  IG +RLV E ++  L YLQ I+SE+LRL P  P
Sbjct: 317 GTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSETLRLYPVAP 376

Query: 181 LLVPHCALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEVDYSY 240
           +L+PH A  E+C ++GYDVPR TIIL+N WAIHRD  +WE+   FKPER     E     
Sbjct: 377 MLLPHLA-SEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEKKGE---DK 436

Query: 241 KLIPFGSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEG-KGLTMPKTRPL 300
           KL+PFG GRR+CPG G+AQR+V L L SL+Q FEW+RV    +DM E  KG TM K   L
Sbjct: 437 KLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTMRKATSL 496

Query: 301 VAKCKPRPIMKIILNA 311
            A CK RPI+  +L+A
Sbjct: 497 QAMCKARPIVHKVLDA 506

BLAST of Bhi10G001254 vs. TAIR10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8)

HSP 1 Score: 319.3 bits (817), Expect = 2.6e-87
Identity = 158/314 (50.32%), Postives = 220/314 (70.06%), Query Frame = 0

Query: 1   MVCGKKFY-----EEDYSNKFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRVKKLA 60
           MV GK++Y     ++  + + R+L+  ++A  GA N  D++P+   +  + +  RVKKLA
Sbjct: 188 MVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRVKKLA 247

Query: 61  KTSDEFLQQLIDEIRNQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGIDTTA 120
              DEFLQ L+DE R   + GNTMI HLL+LQ  +P Y++D+ IKG +  ++LAG DT+A
Sbjct: 248 GRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGTDTSA 307

Query: 121 VTLEWTLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLNPPVP 180
           VTLEW LS +LN+P+VL KA+ EID  IG +RL++E+++ +L YLQ I+SE+LRL P  P
Sbjct: 308 VTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLYPAAP 367

Query: 181 LLVPHCALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEVDYSY 240
           +L+PH A  E+CK+ GYD+PR TI+L NVWAIHRD  +W+D  SFKPER     E   + 
Sbjct: 368 MLLPHVA-SEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKEGE---AQ 427

Query: 241 KLIPFGSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEGKGLTMPKTRPLV 300
           KL+PFG GRRACPG G+A R++ LTL SLIQ  EW+++    +DM+EGKG+TMPK +PL 
Sbjct: 428 KLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAKPLE 487

Query: 301 AKCKPRPIMKIILN 310
           A C+ RP +  I N
Sbjct: 488 AMCRARPSVVKIFN 494

BLAST of Bhi10G001254 vs. Swiss-Prot
Match: sp|Q6WNQ9|C81E9_MEDTR (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 5.6e-87
Identity = 164/312 (52.56%), Postives = 217/312 (69.55%), Query Frame = 0

Query: 1   MVCGKKFYEED-------YSNKFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRVKK 60
           M+ GK++Y +D        + +FRE+++++M+  GA N GDF+PL   +D     KR K+
Sbjct: 188 MISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCKR 247

Query: 61  LAKTSDEFLQQLIDEIR--NQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGI 120
           +AK S+ FL+ LI+E R  N +  G TMI HLL L   +P+YYSD  IKGLIQ +LLAG 
Sbjct: 248 IAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGT 307

Query: 121 DTTAVTLEWTLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLN 180
           DT+AVT+EW +S+LLN+PEVL+KAK E+D+ IG  +LV+E +L  L YLQ IISE+LRL+
Sbjct: 308 DTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLH 367

Query: 181 PPVPLLVPHCALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEV 240
           PP PLL+PH +  E+C I  ++VP++TIIL NVW IHRD   W DA SFKPER     EV
Sbjct: 368 PPAPLLLPHYS-SEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEKEEEV 427

Query: 241 DYSYKLIPFGSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEGKGLTMPKT 300
           +   K++ FG GRRACPG+ +AQR VG T+  LIQ FEW+R +   +DM EGKG+TMP  
Sbjct: 428 N---KVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGITMPMK 487

Query: 301 RPLVAKCKPRPI 304
            PL A CK  PI
Sbjct: 488 IPLRAMCKALPI 495

BLAST of Bhi10G001254 vs. Swiss-Prot
Match: sp|Q9LHA1|C8D11_ARATH (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 4.7e-86
Identity = 165/316 (52.22%), Postives = 211/316 (66.77%), Query Frame = 0

Query: 1   MVCGKKFYEEDYSN-----KFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRVKKLA 60
           MV GK+FY +   N       R+L+ +++  GGA N  D+ P+  ++  T + K VKKLA
Sbjct: 197 MVAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TNYEKHVKKLA 256

Query: 61  KTSDEFLQQLIDEIRNQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGIDTTA 120
              DEFLQ L++E R +   GNTMI HLLSLQ  +P YY+D  IKG+I  ++LAG DT+A
Sbjct: 257 GRVDEFLQSLVNEKRVEKVKGNTMIDHLLSLQETQPDYYTDVIIKGIILVMILAGTDTSA 316

Query: 121 VTLEWTLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLNPPVP 180
            TLEW +S LLN+PEVL KAK EID  IG +RLV E ++  L YLQ I+SE+LRL P  P
Sbjct: 317 GTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSETLRLYPVAP 376

Query: 181 LLVPHCALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEVDYSY 240
           +L+PH A  E+C ++GYDVPR TIIL+N WAIHRD  +WE+   FKPER     E     
Sbjct: 377 MLLPHLA-SEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEKKGE---DK 436

Query: 241 KLIPFGSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEG-KGLTMPKTRPL 300
           KL+PFG GRR+CPG G+AQR+V L L SL+Q FEW+RV    +DM E  KG TM K   L
Sbjct: 437 KLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTMRKATSL 496

Query: 301 VAKCKPRPIMKIILNA 311
            A CK RPI+  +L+A
Sbjct: 497 QAMCKARPIVHKVLDA 506

BLAST of Bhi10G001254 vs. Swiss-Prot
Match: sp|P93147|C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 318.5 bits (815), Expect = 8.0e-86
Identity = 154/311 (49.52%), Postives = 221/311 (71.06%), Query Frame = 0

Query: 1   MVCGKKFYEED-------YSNKFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRVKK 60
           M+ GK++Y ED        +++FR++V++++   GA N  DF+PL  ++D     KR+K 
Sbjct: 187 MISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKD 246

Query: 61  LAKTSDEFLQQLIDEIRNQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGIDT 120
           ++  +D FL+ LI+E R + +  NTMI HLL+LQ+ +P+YY+DQ IKGL   +LLAG D+
Sbjct: 247 ISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDS 306

Query: 121 TAVTLEWTLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLNPP 180
           +AVTLEW++S LLN+PEVL+K K E+D+ +G +RLV+E++LP L+YL+ +I+E+LRL  P
Sbjct: 307 SAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTP 366

Query: 181 VPLLVPHCALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEVDY 240
            PLL+PH +  +EC I GY VP++TI+LIN WAIHRD  +W +AT+FKPER     E++ 
Sbjct: 367 APLLLPH-STSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKGELE- 426

Query: 241 SYKLIPFGSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEGKGLTMPKTRP 300
             KLI FG GRRACPG G+A R + +TLA LIQ F+WK +N   ID+ E  G T+ K  P
Sbjct: 427 --KLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLTKLVP 486

Query: 301 LVAKCKPRPIM 305
           L A CK RP++
Sbjct: 487 LKAMCKSRPVI 493

BLAST of Bhi10G001254 vs. Swiss-Prot
Match: sp|Q0WTF4|C81F3_ARATH (Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.8e-85
Identity = 157/312 (50.32%), Postives = 213/312 (68.27%), Query Frame = 0

Query: 1   MVCGKKFY-----EEDYSNKFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRVKKLA 60
           MV GK++Y      E+ +N F++LV  I    GA +PGD++P       + F K+VK LA
Sbjct: 184 MVTGKRYYGDEVHNEEEANVFKKLVADINDCSGARHPGDYLPFMKMFGGS-FEKKVKALA 243

Query: 61  KTSDEFLQQLIDEIRNQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGIDTTA 120
           +  DE LQ+L++E +   D GNTM++HLLSLQ  EP+YY+D TIKGL+  +++AG DT+A
Sbjct: 244 EAMDEILQRLLEECKRDKD-GNTMVNHLLSLQQNEPEYYTDVTIKGLMLGMMIAGTDTSA 303

Query: 121 VTLEWTLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLNPPVP 180
           VTLEW +S LLN+PE LEKAK+EID  IG ERL++E ++ +L YLQ I+SE+ RL P  P
Sbjct: 304 VTLEWAMSSLLNHPEALEKAKLEIDEKIGQERLIDEPDIANLPYLQNIVSETFRLYPAAP 363

Query: 181 LLVPHCALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERH---ASGIEVD 240
           LLVP     E+ K+ GYDVPR T++++N WAIHRD  +W +   FKPER      G   +
Sbjct: 364 LLVPRSPT-EDIKVGGYDVPRGTMVMVNAWAIHRDPELWNEPEKFKPERFNGGEGGGRGE 423

Query: 241 YSYKLIPFGSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEGKGLTMPKTR 300
             +KL+PFG+GRR+CPG G+ Q++V L L SLIQ F+W++VN   IDM E  G+ M K  
Sbjct: 424 DVHKLMPFGNGRRSCPGAGLGQKIVTLALGSLIQCFDWQKVNGEAIDMTETPGMAMRKKI 483

Query: 301 PLVAKCKPRPIM 305
           PL A C+ RPIM
Sbjct: 484 PLSALCQSRPIM 492

BLAST of Bhi10G001254 vs. Swiss-Prot
Match: sp|O65790|C81F1_ARATH (Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2)

HSP 1 Score: 317.0 bits (811), Expect = 2.3e-85
Identity = 154/314 (49.04%), Postives = 214/314 (68.15%), Query Frame = 0

Query: 1   MVCGKKFYEEDYSNK-----FRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRVKKLA 60
           MV GK++Y +D +NK     F++LV  I  + GA +  D++P+   +    F K VK + 
Sbjct: 189 MVTGKRYYGDDVNNKEEAELFKKLVYDIAMYSGANHSADYLPILK-LFGNKFEKEVKAIG 248

Query: 61  KTSDEFLQQLIDEIRNQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGIDTTA 120
           K+ D+ LQ+L+DE R   + GNTM++HL+SLQ  +P+YY+D  IKGL+  ++LAG +T+A
Sbjct: 249 KSMDDILQRLLDECRRDKE-GNTMVNHLISLQQQQPEYYTDVIIKGLMMSMMLAGTETSA 308

Query: 121 VTLEWTLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLNPPVP 180
           VTLEW ++ LL NPEVLEKA+ EID  IG +RL++E+++  L YLQ ++SE+ RL P  P
Sbjct: 309 VTLEWAMANLLRNPEVLEKARSEIDEKIGKDRLIDESDIAVLPYLQNVVSETFRLFPVAP 368

Query: 181 LLVPHCALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEVD-YS 240
            L+P     ++ KI GYDVPR+TI+++N WAIHRD  IWE+   F P+R+  G   D Y 
Sbjct: 369 FLIPRSPT-DDMKIGGYDVPRDTIVMVNAWAIHRDPEIWEEPEKFNPDRYNDGCGSDYYV 428

Query: 241 YKLIPFGSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEGKGLTMPKTRPL 300
           YKL+PFG+GRR CPG G+ QR+V L L SLIQ FEW+ V    +DM+E  GL M K  PL
Sbjct: 429 YKLMPFGNGRRTCPGAGLGQRIVTLALGSLIQCFEWENVKGEEMDMSESTGLGMRKMDPL 488

Query: 301 VAKCKPRPIMKIIL 309
            A C+PRPIM  +L
Sbjct: 489 RAMCRPRPIMSKLL 499

BLAST of Bhi10G001254 vs. TrEMBL
Match: tr|A0A1S3C323|A0A1S3C323_CUCME (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495924 PE=3 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 3.1e-135
Identity = 232/312 (74.36%), Postives = 270/312 (86.54%), Query Frame = 0

Query: 1   MVCGKKFYEEDYSNKFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRVKKLAKTSDE 60
           MV GK FYEED SNKFRE+VTQIM H GA+NPGDFIPLWNWIDPTGFMK+VKKL KTSDE
Sbjct: 190 MVSGKMFYEED-SNKFREIVTQIMEHAGASNPGDFIPLWNWIDPTGFMKKVKKLGKTSDE 249

Query: 61  FLQQLIDEIRNQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGIDTTAVTLEW 120
           FLQQLID IRNQNDGGNTMIHHLL+LQN+EP++Y+D   K +IQDILLAGIDT+AVTL+W
Sbjct: 250 FLQQLIDGIRNQNDGGNTMIHHLLTLQNVEPEFYNDHAFKAIIQDILLAGIDTSAVTLQW 309

Query: 121 TLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLNPPVPLLVPH 180
            LS LLNNP VLEKAK EIDS IG ER+VNEA+L SLSYLQGIISE+LRL+P  PLLVPH
Sbjct: 310 ALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQGIISETLRLSPAGPLLVPH 369

Query: 181 CALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEVDYSYKLIPF 240
           C+  E+CKI GYDVPRNTI+LIN WAIHRD ++WEDA+SFKPERH +    + SYKL+PF
Sbjct: 370 CS-SEDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKPERHVNAAGFENSYKLLPF 429

Query: 241 GSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEGKGLTMPKTRPLVAKCKP 300
           G GRRACPG+ MAQRV+GLTLASL+Q FEWKR+++ L+DM EG+GLTMPK  PLVAKC+P
Sbjct: 430 GMGRRACPGMAMAQRVIGLTLASLVQCFEWKRMSDLLVDMREGEGLTMPKVEPLVAKCRP 489

Query: 301 RPIMKIILNARS 313
             IM+ +L+ ++
Sbjct: 490 CFIMEAVLSEKN 499

BLAST of Bhi10G001254 vs. TrEMBL
Match: tr|A0A1S3C2H7|A0A1S3C2H7_CUCME (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495922 PE=3 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 3.1e-127
Identity = 229/316 (72.47%), Postives = 260/316 (82.28%), Query Frame = 0

Query: 1   MVCGKKF-----YEEDYSNKFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRVKKLA 60
           M+CGKK+      +E+ S KF+E+V Q++A  GA NPGDFIPLWNWIDPT + KRVKKL 
Sbjct: 189 MMCGKKYCGDDLKDEERSKKFKEMVKQVLAISGAGNPGDFIPLWNWIDPTRYEKRVKKLT 248

Query: 61  KTSDEFLQQLIDEIRNQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGIDTTA 120
           KTSDE +QQLIDEIRNQNDG NTMI HLL LQN +P+ YSDQ IKGLIQ ILL GIDT A
Sbjct: 249 KTSDELIQQLIDEIRNQNDGENTMIQHLLGLQNTQPENYSDQIIKGLIQVILLGGIDTAA 308

Query: 121 VTLEWTLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLNPPVP 180
           VTLEW LS LLNNPEVL+KA  EIDSSIG E LV E +  SLSYLQGIISE+LRLNP  P
Sbjct: 309 VTLEWALSHLLNNPEVLKKAIDEIDSSIGQECLVKEVDSFSLSYLQGIISETLRLNPAAP 368

Query: 181 LLVPHCALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEVDYSY 240
           LLVPHCA FE+CKI GYDVPR+TI+LIN W IHRD S+WEDATSFKPERH +   VD +Y
Sbjct: 369 LLVPHCA-FEDCKIGGYDVPRDTIVLINAWTIHRDPSLWEDATSFKPERHENANGVD-AY 428

Query: 241 KLIPFGSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEGKGLTMPKTRPLV 300
           KL+PFG GRRACPG+GMAQRVV LTLASLIQ FEW+R+ NSL+DM EG+GLTMPK +PL 
Sbjct: 429 KLLPFGLGRRACPGMGMAQRVVALTLASLIQCFEWQRLGNSLVDMTEGEGLTMPKAQPLT 488

Query: 301 AKCKPRPIMKIILNAR 312
           AKC+PRPIMK IL+ +
Sbjct: 489 AKCRPRPIMKKILSMK 502

BLAST of Bhi10G001254 vs. TrEMBL
Match: tr|A0A0A0LQI5|A0A0A0LQI5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 1.0e-125
Identity = 219/323 (67.80%), Postives = 261/323 (80.80%), Query Frame = 0

Query: 1   MVCGKKFYEED-----------YSNKFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMK 60
           MV GKKF +E+           Y  + +ELVTQIMAH G+TNPGDFIPLWNWIDP+G+ K
Sbjct: 189 MVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNWIDPSGYNK 248

Query: 61  RVKKLAKTSDEFLQQLIDEIRNQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLA 120
           R+ K+ +  DE LQ+L+DEIRN+ D GNTMI HLL LQ  +P+YYSD  IKGLIQDIL+A
Sbjct: 249 RIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKGLIQDILIA 308

Query: 121 GIDTTAVTLEWTLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLR 180
           GIDT+AVTL+W LS LLNNP VL+KAK EIDS IG ER+VNE +L SLSYLQGIISE+LR
Sbjct: 309 GIDTSAVTLQWALSHLLNNPIVLDKAKAEIDSYIGQERMVNEVDLSSLSYLQGIISETLR 368

Query: 181 LNPPVPLLVPHCALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGI 240
           L+PP PLLVPHCA  E+CKI GYDVPRNTI+LIN WAIHRD ++WEDA SFKPERH + +
Sbjct: 369 LSPPGPLLVPHCA-SEDCKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKPERHVNAV 428

Query: 241 EVDYSYKLIPFGSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEGKGLTMP 300
             + SYKL+PFG GRRACPG+ MAQRVVGLTLASLIQ FEWK+++N L+DM EG+GLTMP
Sbjct: 429 GFENSYKLLPFGLGRRACPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMREGEGLTMP 488

Query: 301 KTRPLVAKCKPRPIMKIILNARS 313
           K   LVAKC+PR IMK++L  ++
Sbjct: 489 KVESLVAKCRPRFIMKVVLGEKN 510

BLAST of Bhi10G001254 vs. TrEMBL
Match: tr|A0A1S3C1S2|A0A1S3C1S2_CUCME (LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103495921 PE=3 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 6.5e-125
Identity = 220/306 (71.90%), Postives = 257/306 (83.99%), Query Frame = 0

Query: 1   MVCGKKFYE---------EDYSNKFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRV 60
           MV GKKF E         E Y  +F+ELVTQIMAHGG+TNPGDFIPL NWIDP+G+ K +
Sbjct: 189 MVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWIDPSGYTKNI 248

Query: 61  KKLAKTSDEFLQQLIDEIRNQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGI 120
            K+ + +DE LQ L+DEIRN+ D GNTMI HLL LQ  +P+YYSDQ IKGL+QDI+LAGI
Sbjct: 249 MKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQDIILAGI 308

Query: 121 DTTAVTLEWTLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLN 180
           DT+ ++LEW LSQLLNNPEVL+KAK EID  IGH+RLV+E +LPSLSYLQGIISE+LRL+
Sbjct: 309 DTSTISLEWALSQLLNNPEVLKKAKEEIDCLIGHKRLVSEVDLPSLSYLQGIISETLRLH 368

Query: 181 PPVPLLVPHCALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEV 240
           PP PLLVPHCALFEECKI+ Y++PR+TI+LIN WAIHRDS +WEDAT FKPERH S IE 
Sbjct: 369 PPAPLLVPHCALFEECKIKRYNIPRDTIVLINAWAIHRDSGLWEDATEFKPERH-SVIES 428

Query: 241 DYSYKLIPFGSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEGKGLTMPKT 298
           +  YKLIPFG GRRACPG GMAQRVVGL LASLIQSFEW+RV++SLIDM+EGKGLTMPK 
Sbjct: 429 EL-YKLIPFGVGRRACPGAGMAQRVVGLALASLIQSFEWERVDDSLIDMSEGKGLTMPKA 488

BLAST of Bhi10G001254 vs. TrEMBL
Match: tr|A0A0A0LTD0|A0A0A0LTD0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425740 PE=3 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 6.8e-122
Identity = 208/314 (66.24%), Postives = 258/314 (82.17%), Query Frame = 0

Query: 1   MVCGKKFY-----EEDYSNKFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRVKKLA 60
           MV GK+++     +E  + KFR++V ++M + GATNPGDFIPLWNWIDPTG  K++ K+ 
Sbjct: 190 MVGGKRYFGDDVLDEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTGLEKKIMKVG 249

Query: 61  KTSDEFLQQLIDEIRNQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGIDTTA 120
           + +DE  Q LIDEIRN+ + GNTMIHHLL LQN +P+Y+SDQ IKGLI  I LAGIDT +
Sbjct: 250 EEADEIFQGLIDEIRNEEEDGNTMIHHLLHLQNTQPEYFSDQIIKGLIHVIFLAGIDTIS 309

Query: 121 VTLEWTLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLNPPVP 180
           VTLEW LS LLNNP+V++KA++EI+  +G ERLVNE +L SLSYLQGII E+LRL P  P
Sbjct: 310 VTLEWGLSHLLNNPKVIKKARLEIEHIVGQERLVNEDDLSSLSYLQGIILETLRLTPAAP 369

Query: 181 LLVPHCALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEVDYSY 240
           LLVPHCA  E+C+IEGYD+PR TII +N WAIHRDSS+WED TSFKPERH + IE+  SY
Sbjct: 370 LLVPHCA-SEDCQIEGYDIPRGTIIFVNAWAIHRDSSLWEDVTSFKPERHENAIELSDSY 429

Query: 241 KLIPFGSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEGKGLTMPKTRPLV 300
           KL+PFG GRRACPGVGMAQRV+GLTLASLIQ F+W+R+++SL+DM EG+G+TMPK +PLV
Sbjct: 430 KLLPFGLGRRACPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGITMPKAQPLV 489

Query: 301 AKCKPRPIMKIILN 310
           AKCKPRPIM+  L+
Sbjct: 490 AKCKPRPIMRAHLS 502

BLAST of Bhi10G001254 vs. NCBI nr
Match: XP_022943757.1 (isoflavone 2'-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 507.3 bits (1305), Expect = 3.7e-140
Identity = 243/308 (78.90%), Postives = 279/308 (90.58%), Query Frame = 0

Query: 1   MVCGKKFYEEDYSNKFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRVKKLAKTSDE 60
           MV GK+FYE    +KFRE+VTQIMAH GATNPGDFIPLW +IDP+GF KRVKKL KTSDE
Sbjct: 187 MVAGKRFYER--HDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLGKTSDE 246

Query: 61  FLQQLIDEIRNQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGIDTTAVTLEW 120
            LQ+L+DEIR++NDGGNTMIH LLSLQN EP+YYSDQ IKGLIQDILLAGIDT AVTLEW
Sbjct: 247 LLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTLAVTLEW 306

Query: 121 TLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLNPPVPLLVPH 180
           T+SQLLN+PEVLEKA+ EIDSSIG+ERLVNE++LPSLSY+QGIISE+LRL+P  PLL+PH
Sbjct: 307 TVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAPLLLPH 366

Query: 181 CALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEVD-YSYKLIP 240
           CA FEECKIEGYDVPR+TII IN WA+HRD ++WEDATSFKPERHA+G++    SYKLIP
Sbjct: 367 CA-FEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYKLIP 426

Query: 241 FGSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEGKGLTMPKTRPLVAKCK 300
           FG GRRACPG+GMAQRV GLTLASLIQ FEW+R+NNSL+DM+EGKG+TMPK +PLVAKCK
Sbjct: 427 FGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVAKCK 486

Query: 301 PRPIMKII 308
           PRPIMK++
Sbjct: 487 PRPIMKLV 491

BLAST of Bhi10G001254 vs. NCBI nr
Match: XP_023512186.1 (isoflavone 2'-hydroxylase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023512187.1 isoflavone 2'-hydroxylase-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 505.4 bits (1300), Expect = 1.4e-139
Identity = 242/308 (78.57%), Postives = 277/308 (89.94%), Query Frame = 0

Query: 1   MVCGKKFYEEDYSNKFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRVKKLAKTSDE 60
           MV GK+FYE    +KFRE+VTQIMAH GATNPGDFIPLW +IDP+GF KRVKKL KT DE
Sbjct: 187 MVAGKRFYER--HDKFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLGKTCDE 246

Query: 61  FLQQLIDEIRNQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGIDTTAVTLEW 120
            L++L+DEIR++NDGGNTMIH LLSLQN EP+YYSDQ IKGLIQDILLAGIDT AVTLEW
Sbjct: 247 ILRELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTLAVTLEW 306

Query: 121 TLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLNPPVPLLVPH 180
            LSQLLN+PEVLEKA+ EID+SIG+ERLVNE +LP+LSY+QGIISE++RLNP  PLLVPH
Sbjct: 307 ALSQLLNHPEVLEKARAEIDNSIGYERLVNEVDLPNLSYVQGIISETMRLNPAAPLLVPH 366

Query: 181 CALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEVDY-SYKLIP 240
           CA FEECKIEGYDVPR+TIILIN WA+HRD ++WEDATSFKPERHA+G++    SYKLIP
Sbjct: 367 CA-FEECKIEGYDVPRDTIILINAWAMHRDPNLWEDATSFKPERHANGMKNGADSYKLIP 426

Query: 241 FGSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEGKGLTMPKTRPLVAKCK 300
           FG GRRACPGVGMAQRV GLTLASLIQ FEW+R+NNSL+DM+EGKG+TMPK +PLVAKCK
Sbjct: 427 FGLGRRACPGVGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVAKCK 486

Query: 301 PRPIMKII 308
           PRPIMK++
Sbjct: 487 PRPIMKLV 491

BLAST of Bhi10G001254 vs. NCBI nr
Match: XP_022943410.1 (isoflavone 2'-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 500.0 bits (1286), Expect = 6.0e-138
Identity = 241/308 (78.25%), Postives = 276/308 (89.61%), Query Frame = 0

Query: 1   MVCGKKFYEEDYSNKFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRVKKLAKTSDE 60
           MV GKK + E +S KF+E+VTQIMAH GATNP DFIPLW +IDPTGF KR KKLAKTSDE
Sbjct: 187 MVAGKKSF-EGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLAKTSDE 246

Query: 61  FLQQLIDEIRNQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGIDTTAVTLEW 120
            LQ+L+DEIR++ DGGNTMIH LLSLQN EP+YYSDQ IKGLIQDILLAGIDT AVTLEW
Sbjct: 247 LLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTIAVTLEW 306

Query: 121 TLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLNPPVPLLVPH 180
            +SQLLNNPEVLEKA+ EIDSSIG+ERLVNE++LPSLSY+QGIISE+LRL+P  PLL+PH
Sbjct: 307 AMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAPLLLPH 366

Query: 181 CALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEVD-YSYKLIP 240
           CA FEECKIEGYDVPR+TII IN WA+HRD ++WEDATSFKPERHA+G++    SYKLIP
Sbjct: 367 CA-FEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYKLIP 426

Query: 241 FGSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEGKGLTMPKTRPLVAKCK 300
           FG GRRACPG+GMAQRV GLTLASLIQ FEW+R+NNSL+DM+EGKG+TMPK +PLVAKCK
Sbjct: 427 FGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVAKCK 486

Query: 301 PRPIMKII 308
           PRPIMK++
Sbjct: 487 PRPIMKLV 492

BLAST of Bhi10G001254 vs. NCBI nr
Match: XP_022985609.1 (isoflavone 2'-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 495.4 bits (1274), Expect = 1.5e-136
Identity = 240/308 (77.92%), Postives = 274/308 (88.96%), Query Frame = 0

Query: 1   MVCGKKFYEEDYSNKFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRVKKLAKTSDE 60
           M+ GKK + E +S KF+E+VT IMAH GATNP DFIPLW +IDPTGF KR KKLAKTSDE
Sbjct: 187 MLAGKKSF-EGHSYKFKEMVTHIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLAKTSDE 246

Query: 61  FLQQLIDEIRNQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGIDTTAVTLEW 120
            LQ+L+DEIR++ DGGNTMIH LLSLQN EP+YYSDQ IKGLIQDILLAGIDT  VTLEW
Sbjct: 247 LLQELLDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTLVVTLEW 306

Query: 121 TLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLNPPVPLLVPH 180
            LSQLLNNPEVLEKA+ EIDSSIG+ERLVNEA+LPSLSY+QGIISE+LRL+P  PLL+PH
Sbjct: 307 ALSQLLNNPEVLEKARAEIDSSIGYERLVNEADLPSLSYVQGIISETLRLHPAAPLLLPH 366

Query: 181 CALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEVD-YSYKLIP 240
           CA  EECKIEGYDVPR+TIILIN WA+HRD ++WEDATSFKPERHA+G++    SYKLIP
Sbjct: 367 CA-SEECKIEGYDVPRDTIILINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYKLIP 426

Query: 241 FGSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEGKGLTMPKTRPLVAKCK 300
           FG GRRACPG+GMAQRV GLTLASLIQ FEW+R+NNSL+DM+EGKG+TMPK +PLVAKCK
Sbjct: 427 FGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVAKCK 486

Query: 301 PRPIMKII 308
           PRPIMK++
Sbjct: 487 PRPIMKLV 492

BLAST of Bhi10G001254 vs. NCBI nr
Match: XP_008455824.1 (PREDICTED: cytochrome P450 81D1-like [Cucumis melo])

HSP 1 Score: 490.3 bits (1261), Expect = 4.7e-135
Identity = 232/312 (74.36%), Postives = 270/312 (86.54%), Query Frame = 0

Query: 1   MVCGKKFYEEDYSNKFRELVTQIMAHGGATNPGDFIPLWNWIDPTGFMKRVKKLAKTSDE 60
           MV GK FYEED SNKFRE+VTQIM H GA+NPGDFIPLWNWIDPTGFMK+VKKL KTSDE
Sbjct: 190 MVSGKMFYEED-SNKFREIVTQIMEHAGASNPGDFIPLWNWIDPTGFMKKVKKLGKTSDE 249

Query: 61  FLQQLIDEIRNQNDGGNTMIHHLLSLQNIEPQYYSDQTIKGLIQDILLAGIDTTAVTLEW 120
           FLQQLID IRNQNDGGNTMIHHLL+LQN+EP++Y+D   K +IQDILLAGIDT+AVTL+W
Sbjct: 250 FLQQLIDGIRNQNDGGNTMIHHLLTLQNVEPEFYNDHAFKAIIQDILLAGIDTSAVTLQW 309

Query: 121 TLSQLLNNPEVLEKAKVEIDSSIGHERLVNEANLPSLSYLQGIISESLRLNPPVPLLVPH 180
            LS LLNNP VLEKAK EIDS IG ER+VNEA+L SLSYLQGIISE+LRL+P  PLLVPH
Sbjct: 310 ALSHLLNNPIVLEKAKAEIDSYIGQERMVNEADLSSLSYLQGIISETLRLSPAGPLLVPH 369

Query: 181 CALFEECKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEVDYSYKLIPF 240
           C+  E+CKI GYDVPRNTI+LIN WAIHRD ++WEDA+SFKPERH +    + SYKL+PF
Sbjct: 370 CS-SEDCKIGGYDVPRNTIVLINAWAIHRDPNLWEDASSFKPERHVNAAGFENSYKLLPF 429

Query: 241 GSGRRACPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEGKGLTMPKTRPLVAKCKP 300
           G GRRACPG+ MAQRV+GLTLASL+Q FEWKR+++ L+DM EG+GLTMPK  PLVAKC+P
Sbjct: 430 GMGRRACPGMAMAQRVIGLTLASLVQCFEWKRMSDLLVDMREGEGLTMPKVEPLVAKCRP 489

Query: 301 RPIMKIILNARS 313
             IM+ +L+ ++
Sbjct: 490 CFIMEAVLSEKN 499