Bhi10G001171 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi10G001171
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	Cytochrome P450
Location	chr10 : 36035241 .. 36036110 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATATTCTCTTCCTTTTCATTTCCCTCTCTCTATTCTCTCTCCTTGCCTTGAGATTTTTTCTTCAACTTCATCGCCAAAACCTTCCCCCAACACCACTTTTCTCTCTTCCCATTATTGGTCATCTCCATCTTGTCAAACATCCTATCCATAGAATCCTTCACAATCTCTCCAAAAAGTATGGAGATGTCTTCTCTCTCCGATTCGGTTCACGACTCGTCGTAGTTGTATCATCCCCTTCTGTTGTCCAAGAATGTCTTACAAAGAACGATATCATTCTCGCAAATCGACCCTTGTTGGGCACTGGAAAGCACTTGGCATACAACCATACCACCATGGTTGTTGCCCCTTATGGTGAACACTGGCGAAACCTTCGTCGAATTAGCGCCCTCGAGCTTTTCTCGACGAATCGACTAAATCTATTCTCGGGAATTCGAGAAGACGAAGTTAAAGGGTTGATGGTTCGATTACGTGGGAGTTCGTTGGAAGAATTCGCACTAGTAGAGCCAGAAAGTATGTTTTTAGATCTGATGTACAATGCTATTATAAGAATGGTGGGTGGGAAGAAGCCTTGTGAAGAGGATAAGGGAAATTCGAGGGAATTTAGAGAGGTGGTGACAAAGATTATGGAGGTTGGTGGGGCAACAAATCCAGGAGACTTCATACCAATATGGAATTGGATTGATCCGTGTGGCTTGGAGAAGAAGATCAAAAAGCTTGGAAAGACAATGGATGAACTTCTCCAAGACTTAGTTGATGGGGTAAGAAATGAGAAAGGTGAAGGGAATGCTATGATTCATCGTTTACTTCATTTGCAGAAAACTGAGCCTGAGAATTATAGTGACCAGATTATCAAAGGCCTAATACAG

mRNA sequence

ATGGATATTCTCTTCCTTTTCATTTCCCTCTCTCTATTCTCTCTCCTTGCCTTGAGATTTTTTCTTCAACTTCATCGCCAAAACCTTCCCCCAACACCACTTTTCTCTCTTCCCATTATTGGTCATCTCCATCTTGTCAAACATCCTATCCATAGAATCCTTCACAATCTCTCCAAAAAGTATGGAGATGTCTTCTCTCTCCGATTCGGTTCACGACTCGTCGTAGTTGTATCATCCCCTTCTGTTGTCCAAGAATGTCTTACAAAGAACGATATCATTCTCGCAAATCGACCCTTGTTGGGCACTGGAAAGCACTTGGCATACAACCATACCACCATGGTTGTTGCCCCTTATGGTGAACACTGGCGAAACCTTCGTCGAATTAGCGCCCTCGAGCTTTTCTCGACGAATCGACTAAATCTATTCTCGGGAATTCGAGAAGACGAAGTTAAAGGGTTGATGGTTCGATTACGTGGGAGTTCGTTGGAAGAATTCGCACTAGTAGAGCCAGAAAGTATGTTTTTAGATCTGATGTACAATGCTATTATAAGAATGGTGGGTGGGAAGAAGCCTTGTGAAGAGGATAAGGGAAATTCGAGGGAATTTAGAGAGGTGGTGACAAAGATTATGGAGGTTGGTGGGGCAACAAATCCAGGAGACTTCATACCAATATGGAATTGGATTGATCCGTGTGGCTTGGAGAAGAAGATCAAAAAGCTTGGAAAGACAATGGATGAACTTCTCCAAGACTTAGTTGATGGGGTAAGAAATGAGAAAGGTGAAGGGAATGCTATGATTCATCGTTTACTTCATTTGCAGAAAACTGAGCCTGAGAATTATAGTGACCAGATTATCAAAGGCCTAATACAG

Coding sequence (CDS)

ATGGATATTCTCTTCCTTTTCATTTCCCTCTCTCTATTCTCTCTCCTTGCCTTGAGATTTTTTCTTCAACTTCATCGCCAAAACCTTCCCCCAACACCACTTTTCTCTCTTCCCATTATTGGTCATCTCCATCTTGTCAAACATCCTATCCATAGAATCCTTCACAATCTCTCCAAAAAGTATGGAGATGTCTTCTCTCTCCGATTCGGTTCACGACTCGTCGTAGTTGTATCATCCCCTTCTGTTGTCCAAGAATGTCTTACAAAGAACGATATCATTCTCGCAAATCGACCCTTGTTGGGCACTGGAAAGCACTTGGCATACAACCATACCACCATGGTTGTTGCCCCTTATGGTGAACACTGGCGAAACCTTCGTCGAATTAGCGCCCTCGAGCTTTTCTCGACGAATCGACTAAATCTATTCTCGGGAATTCGAGAAGACGAAGTTAAAGGGTTGATGGTTCGATTACGTGGGAGTTCGTTGGAAGAATTCGCACTAGTAGAGCCAGAAAGTATGTTTTTAGATCTGATGTACAATGCTATTATAAGAATGGTGGGTGGGAAGAAGCCTTGTGAAGAGGATAAGGGAAATTCGAGGGAATTTAGAGAGGTGGTGACAAAGATTATGGAGGTTGGTGGGGCAACAAATCCAGGAGACTTCATACCAATATGGAATTGGATTGATCCGTGTGGCTTGGAGAAGAAGATCAAAAAGCTTGGAAAGACAATGGATGAACTTCTCCAAGACTTAGTTGATGGGGTAAGAAATGAGAAAGGTGAAGGGAATGCTATGATTCATCGTTTACTTCATTTGCAGAAAACTGAGCCTGAGAATTATAGTGACCAGATTATCAAAGGCCTAATACAG

Protein sequence

MDILFLFISLSLFSLLALRFFLQLHRQNLPPTPLFSLPIIGHLHLVKHPIHRILHNLSKKYGDVFSLRFGSRLVVVVSSPSVVQECLTKNDIILANRPLLGTGKHLAYNHTTMVVAPYGEHWRNLRRISALELFSTNRLNLFSGIREDEVKGLMVRLRGSSLEEFALVEPESMFLDLMYNAIIRMVGGKKPCEEDKGNSREFREVVTKIMEVGGATNPGDFIPIWNWIDPCGLEKKIKKLGKTMDELLQDLVDGVRNEKGEGNAMIHRLLHLQKTEPENYSDQIIKGLIQ

Homology

[Close]

BLAST of Bhi10G001171 vs. TAIR10
Match: AT4G37310.1 (cytochrome P450, family 81, subfamily H, polypeptide 1)

HSP 1 Score: 270.8 bits (691), Expect = 9.9e-73
Identity = 150/296 (50.68%), Postives = 193/296 (65.20%), Query Frame = 0


Query: 3   ILFLFISLSLFSLLALRFFLQLHRQNLPPTPLFSLPIIGHLHLVKHP-IHRILHNLSKKY 62
           IL +  +L    ++ +   +    +NLPP+P    PIIGHLHL+K P +HR L +LS   
Sbjct: 4   ILLILTTLVAIFIVKIVLLVTKPNKNLPPSPNICFPIIGHLHLLKKPLLHRTLSHLSHSL 63

Query: 63  GDVFSLRFGSRLVVVVSSPSVVQEC-LTKNDIILANRPLLGTGKHLAYNHTTMVVAPYGE 122
           G VFSLR GSRL V++SSP+  +EC LTKNDI+LANRP    GK++AY++T+MV APYG+
Sbjct: 64  GPVFSLRLGSRLAVIISSPTAAEECFLTKNDIVLANRPRFIMGKYVAYDYTSMVTAPYGD 123

Query: 123 HWRNLRRISALELFSTNRLNLFSGIREDEVKGLMVRLRGSSLEEFALVEPESMFLDLMYN 182
           HWRNLRRI+ALE+FSTNRLN  + IR DEVK L+ +L   S+E  A VE   +   L  N
Sbjct: 124 HWRNLRRITALEVFSTNRLNASAEIRHDEVKMLLQKLHDLSVERPAKVELRQLLTGLTLN 183

Query: 183 AIIRMVGGKKPCEEDKGN----SREFREVVTKIMEVGGATNPGDFIPIWNWIDPCGLEKK 242
            I+RM+ GK+  EED G     S EFRE+V +I+E+  A NP DF+P   W D  GL K+
Sbjct: 184 VIMRMMTGKRFFEEDDGGKAGISLEFRELVAEILELSAADNPADFLPALRWFDYKGLVKR 243

Query: 243 IKKLGKTMDELLQDLVDGVRNEKGE---GNAMIHRLLHLQKTEPENYSDQIIKGLI 290
            K++G+ MD LLQ  +D  R  K      N MI  LL  Q+ EP NYSDQ IKGLI
Sbjct: 244 AKRIGERMDSLLQGFLDEHRANKDRLEFKNTMIAHLLDSQEKEPHNYSDQTIKGLI 299

[Close]

BLAST of Bhi10G001171 vs. TAIR10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5)

HSP 1 Score: 260.8 bits (665), Expect = 1.0e-69
Identity = 134/290 (46.21%), Postives = 191/290 (65.86%), Query Frame = 0


Query: 5   FLFISLSLFSLLALRFFLQLHRQNLPPTPLFSLPIIGHLHLVKHPIHRILHNLSKKYGD- 64
           FLF+++S+  LL         + NLPP+P + LP+IGHLHL+K P+HR  H++SK  G+ 
Sbjct: 11  FLFLTISIKLLLT----KSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNA 70

Query: 65  -VFSLRFGSRLVVVVSSPSVVQECLTKNDIILANRPLLGTGKHLAYNHTTMVVAPYGEHW 124
            +F LR G+RLV V+SS S+ +EC TKND++LANRP +   KH+ YN T M+ A YG+HW
Sbjct: 71  PIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHW 130

Query: 125 RNLRRISALELFSTNRLNLFSGIREDEVKGLMVRLRGSSLEEFALVEPESMFLDLMYNAI 184
           RNLRRI+A+E+FS++R++ FS IR+DE++ L+  L   SL  F  VE +S+  +L +N I
Sbjct: 131 RNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAFNNI 190

Query: 185 IRMVGGKK---PCEEDKGNSREFREVVTKIMEVGGATNPGDFIPIWNWIDPCGLEKKIKK 244
           I MV GK+      ED   ++  RE++ +IM   G+ N  D++P  NW+     E + K 
Sbjct: 191 IMMVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--TNFENQTKI 250

Query: 245 LGKTMDELLQDLVDGVRNEKGEGNAMIHRLLHLQKTEPENYSDQIIKGLI 290
           LG  +D +LQ LVD  R EK +G  +I  LL  Q+TEPE Y+D IIKG+I
Sbjct: 251 LGNRLDRVLQKLVDEKRAEKEKGQTLIDHLLSFQETEPEYYTDVIIKGII 294

[Close]

BLAST of Bhi10G001171 vs. TAIR10
Match: AT2G23190.1 (cytochrome P450, family 81, subfamily D, polypeptide 7)

HSP 1 Score: 254.2 bits (648), Expect = 9.6e-68
Identity = 139/297 (46.80%), Postives = 193/297 (64.98%), Query Frame = 0


Query: 1   MDILFLFISLSLFSLLALR-FFLQLHRQ-NLPPTPLFSLPIIGHLHLVKHPIHRILHNLS 60
           M+  FL +SL+    ++L+  F + H + NLPP+P   LP IGHLHL+K P+HR   + S
Sbjct: 45  METHFLILSLAFLFFISLKLLFGKRHSKFNLPPSPARPLPFIGHLHLLKQPLHRTFLSFS 104

Query: 61  KKYGD--VFSLRFGSRLVVVVSSPSVVQECLTKNDIILANRPLLGTGKHLAYNHTTMVVA 120
           +  GD  +FSLR G+ L VVVSS S+ +EC TKNDI+LANRP    GKH+ YN TTM  A
Sbjct: 105 QSLGDAPIFSLRLGNHLTVVVSSYSIAEECFTKNDIVLANRPKFILGKHIEYNFTTMTSA 164

Query: 121 PYGEHWRNLRRISALELFSTNRLNLFSGIREDEVKGLMVRLRGSSLEEFALVEPESMFLD 180
           PYG+HWRNLRRI  LE+FS+++LN F  +R+DE++ L++RL  +S   FA VE   +F D
Sbjct: 165 PYGDHWRNLRRIGTLEIFSSHKLNGFLSVRKDEIRHLLLRLSKNSQHGFAKVEMRQLFYD 224

Query: 181 LMYNAIIRMVGGKK----PCEEDKGNSREFREVVTKIMEVGGATNPGDFIPIWNWIDPCG 240
           L  N I+RMV GK+      E+D+  +R   +++ +I+   G  N  D+IPI  WI    
Sbjct: 225 LTINNILRMVAGKRFYGEGTEQDE-VARRVTQLIDEIVYRAGVGNAADYIPILRWI--TD 284

Query: 241 LEKKIKKLGKTMDELLQDLVDGVRNEKGEGNAMIHRLLHLQKTEPENYSDQIIKGLI 290
            EK +K+L   +DE LQ LVD  R  K +GN M+  LL LQ+T+P+ Y+D  +KG+I
Sbjct: 285 FEKGVKELASRVDEFLQSLVDERRVHKQKGNTMMDHLLSLQETQPDYYTDVTLKGII 338

[Close]

BLAST of Bhi10G001171 vs. TAIR10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4)

HSP 1 Score: 252.3 bits (643), Expect = 3.6e-67
Identity = 139/294 (47.28%), Postives = 188/294 (63.95%), Query Frame = 0


Query: 1   MDILFLFISLSLFSLLALRFFL---QLHRQNLPPTPLFSLPIIGHLHLVKHPIHRILHNL 60
           MD+  + I LS  SL  L  FL      + NLPP+P  SLP+IGHLHL+K P+HR   +L
Sbjct: 1   MDLNQILI-LSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSL 60

Query: 61  SKKYGD--VFSLRFGSRLVVVVSSPSVVQECLTKNDIILANRPLLGTGKHLAYNHTTMVV 120
           SK  G+  VF LR G+RLV V+SS S+ +EC TKND++LANRP     KHL YN T ++ 
Sbjct: 61  SKSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLS 120

Query: 121 APYGEHWRNLRRISALELFSTNRLNLFSGIREDEVKGLMVRLRGSSLEEFALVEPESMFL 180
           A YG+HWRNLRRI+A+E+FST+RLN F  IR+DE++ L+  L   SL  F  VE +++  
Sbjct: 121 ASYGDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLT 180

Query: 181 DLMYNAIIRMVGGKKPCEEDKGNSREFREVVTKIMEVGGATNPGDFIPIWNWIDPCGLEK 240
           +L  N  IRM+ GK+   ED  +++  + +V++ +   GA NP D++ I  W+     EK
Sbjct: 181 NLASNTTIRMLAGKRYFGEDNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWVS--SYEK 240

Query: 241 KIKKLGKTMDELLQDLVDGVRNEKGEGNAMIHRLLHLQKTEPENYSDQIIKGLI 290
           +IK LG   D  LQ LVD  R EK +G  MI  LL LQ  +P+ Y+D IIKG+I
Sbjct: 241 RIKNLGNRFDTFLQKLVDEKRAEKEKGETMIDHLLALQDIQPDYYTDVIIKGII 291

[Close]

BLAST of Bhi10G001171 vs. TAIR10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3)

HSP 1 Score: 249.6 bits (636), Expect = 2.4e-66
Identity = 141/291 (48.45%), Postives = 190/291 (65.29%), Query Frame = 0


Query: 5   FLFISLSLFSLLALRFFLQLHRQNLPPTPLFSLPIIGHLHLVKHPIHRILHNLSKKYGD- 64
           FLFISLSL  ++         R NLPP+P ++LP+IGHL L+K P+HR+  ++S+  GD 
Sbjct: 10  FLFISLSLTFIIG----RIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDA 69

Query: 65  -VFSLRFGSRLVVVVSSPSVVQECLTKNDIILANRPLLGTGKHLAYNHTTMVVAPYGEHW 124
            + SLR G+RLV VVSS S+ +EC TKND++LANR      KH++Y  TT+V A YG+HW
Sbjct: 70  PIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHW 129

Query: 125 RNLRRISALELFSTNRLNLFSGIREDEVKGLMVRLRGSSLEEFALVEPESMFLDLMYNAI 184
           RNLRRI A+E+FS +RLN FS IR DE+  L+  L  +S  EF  VE +SMF +L +N I
Sbjct: 130 RNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNI 189

Query: 185 IRMVGGK---KPCEEDKGNSREFREVVTKIMEVGGATNPGDFIPIWNWIDPCGLEKKIKK 244
           IRM+ GK       ED   ++  RE++ + M   GA N  D++PI  WI   G EK+IKK
Sbjct: 190 IRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI--TGSEKRIKK 249

Query: 245 LGKTMDELLQDLVDGVRNEKGE-GNAMIHRLLHLQKTEPENYSDQIIKGLI 290
           +   +DE LQ LVD  R  K +  N M+  LL LQ+T+PE Y+D IIKG++
Sbjct: 250 IASRLDEFLQGLVDERREGKEKRQNTMVDHLLCLQETQPEYYTDNIIKGIM 294

[Close]

BLAST of Bhi10G001171 vs. Swiss-Prot
Match: sp|Q6WNQ8|C81E8_MEDTR (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 2.6e-70
Identity = 144/297 (48.48%), Postives = 195/297 (65.66%), Query Frame = 0


Query: 1   MDILFLFISLSLFSL-LALRFFLQLHR--QNLPPTPLFSLPIIGHLHLVKHPIHRILHNL 60
           M   +L + +SLF L + L+ F    R  +NLPP P   LPIIG+LH +K P+H   H L
Sbjct: 1   MTTFYLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQ-CLPIIGNLHQLKQPLHHTFHTL 60

Query: 61  SKKYGDVFSLRFGSRLVVVVSSPSVVQECLTKNDIILANRPLLGTGKHLAYNHTTMVVAP 120
           S+KYG +FSL FGSRLVVVVSS ++ QEC TKNDI+LANRP   TGK++ YN+TT+  +P
Sbjct: 61  SQKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSP 120

Query: 121 YGEHWRNLRRISALELFSTNRLNLFSGIREDEVKGLMVRLRGSSLEEFALVEPESMFLDL 180
           YG+HWRNLRRI ++E+ S++RLN F  IR DE+  L+ +L   S   F  VE   MF ++
Sbjct: 121 YGDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEM 180

Query: 181 MYNAIIRMVGGKK----PCE-EDKGNSREFREVVTKIMEVGGATNPGDFIPIWNWIDPCG 240
            +N I+RMV GK+     C+  D   +R FR ++ +++ +GGA N GDF+    W D  G
Sbjct: 181 TFNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDG 240

Query: 241 LEKKIKKLGKTMDELLQDLVDGVRNEKGEGNAMIHRLLHLQKTEPENYSDQIIKGLI 290
           LEK++KK+ K  D  LQ L+D  R  K   N MI  LL  Q+++PE Y+DQIIKGL+
Sbjct: 241 LEKRLKKISKRTDAFLQGLIDEHRFGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLM 296

[Close]

BLAST of Bhi10G001171 vs. Swiss-Prot
Match: sp|P93147|C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 255.4 bits (651), Expect = 7.7e-67
Identity = 142/297 (47.81%), Postives = 196/297 (65.99%), Query Frame = 0


Query: 1   MDILFLFISLSLFSLLALRFFLQLHR----QNLPPTPLFSLPIIGHLHLVKHPIHRILHN 60
           M+IL L +S S+F L     F  + R    +NLPP P  SLPIIG+LH +K P+HR    
Sbjct: 1   MEILSL-LSYSVFYLALFFIFNIVIRARKFKNLPPGPP-SLPIIGNLHHLKRPLHRTFKG 60

Query: 61  LSKKYGDVFSLRFGSRLVVVVSSPSVVQECLTKNDIILANRPLLGTGKHLAYNHTTMVVA 120
           LS+KYG VFSL FGSRLVVVVSS S  Q+C TKND++LANRP   +GK++ YN+TT+   
Sbjct: 61  LSEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGST 120

Query: 121 PYGEHWRNLRRISALELFSTNRLNLFSGIREDEVKGLMVRLRGSSLEEFALVEPESMFLD 180
            YGEHWRNLRRI+AL++ S +R+N FSGIR DE + L+ RL   S   FA +E  S   D
Sbjct: 121 SYGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYD 180

Query: 181 LMYNAIIRMVGGKKPCEEDKGNS-----REFREVVTKIMEVGGATNPGDFIPIWNWIDPC 240
           + +N I+RM+ GK+   ED   S      +FR++V++++++ GA N  DF+P+  ++D  
Sbjct: 181 MTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFE 240

Query: 241 GLEKKIKKLGKTMDELLQDLVDGVRNEKGEGNAMIHRLLHLQKTEPENYSDQIIKGL 289
            LEK++K +    D  L+ L++  R +K   N MI  LL+LQ ++PE Y+DQIIKGL
Sbjct: 241 NLEKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGL 295

[Close]

BLAST of Bhi10G001171 vs. Swiss-Prot
Match: sp|Q6WNQ9|C81E9_MEDTR (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 6.6e-66
Identity = 135/300 (45.00%), Postives = 196/300 (65.33%), Query Frame = 0


Query: 4   LFLFISLSLFSLLALRFFLQL---HRQNLPPTPLFSLPIIGHLHLVKHPIHRILHNLSKK 63
           LF +  LSL  ++ ++  L++     +NLPP P  ++PIIG+LH +KHP+HR    LS+ 
Sbjct: 2   LFYYSLLSLSFIITIKILLKITSRRLKNLPPGPP-TIPIIGNLHHLKHPLHRTFTTLSQT 61

Query: 64  YGDVFSLRFGSRLVVVVSSPSVVQECLTKNDIILANRPLLGTGKHLAYNHTTMVVAPYGE 123
           YGD+FSL FGSRLVVVVSSPS+  EC TKNDIILANRP   TGK++ YN+TT+  A YG+
Sbjct: 62  YGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGD 121

Query: 124 HWRNLRRISALELFSTNRLNLFSGIREDEVKGLMVRLRGSSLEE---FALVEPESMFLDL 183
           HWRNLRRI+ +++ S NRLN F G+R DE   L+ +L    + E   F  VE      ++
Sbjct: 122 HWRNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEM 181

Query: 184 MYNAIIRMVGGKKPCEEDKG-----NSREFREVVTKIMEVGGATNPGDFIPIWNWIDPCG 243
            +NA++RM+ GK+   +D        +++FRE+++++M + GA N GDF+P+   +D   
Sbjct: 182 TFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDN 241

Query: 244 LEKKIKKLGKTMDELLQDLVDGVR--NEKGEGNAMIHRLLHLQKTEPENYSDQIIKGLIQ 291
           LEK+ K++ K  +  L+ L++  R  N   +G  MI  LL L +++PE YSD +IKGLIQ
Sbjct: 242 LEKRCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQ 300

[Close]

BLAST of Bhi10G001171 vs. Swiss-Prot
Match: sp|Q0WTF4|C81F3_ARATH (Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 4.7e-64
Identity = 133/289 (46.02%), Postives = 192/289 (66.44%), Query Frame = 0


Query: 6   LFISLSLFSLLALRFFL-QLHRQNLPPTPLFSLPIIGHLHLVKHPIHRILHNLSKKYGDV 65
           + + L+LF L    FF  +  R NLPP+P +SLPI+GH +L+K P+HR+ H LSK +G +
Sbjct: 6   IVLPLALFLLAYKLFFTSKTKRFNLPPSPPYSLPILGHHNLLKPPVHRLFHRLSKTHGPI 65

Query: 66  FSLRFGSRLVVVVSSPSVVQECLT-KNDIILANRPLLGTGKHLAYNHTTMVVAPYGEHWR 125
           FSL+FGSR  VV+SS S+  +C T +NDIIL+NRP   T K++AYN+TT+  APYG+HWR
Sbjct: 66  FSLQFGSRRAVVISSSSLATQCFTGQNDIILSNRPCFLTAKYVAYNYTTVGTAPYGDHWR 125

Query: 126 NLRRISALELFSTNRLNLFSGIREDEVKGLMVRLRGSSLEEFALVEPESMFLDLMYNAII 185
           NLRRI +LE+ S+NRL  F  IR+DE+  ++ RL     +E   +E E +  DL +N I+
Sbjct: 126 NLRRICSLEILSSNRLTNFLHIRKDEIHRMLTRLSRDVNKE---IELEPLLSDLTFNNIV 185

Query: 186 RMVGGKKPCEEDKGNSRE---FREVVTKIMEVGGATNPGDFIPIWNWIDPCGLEKKIKKL 245
           RMV GK+   ++  N  E   F+++V  I +  GA +PGD++P          EKK+K L
Sbjct: 186 RMVTGKRYYGDEVHNEEEANVFKKLVADINDCSGARHPGDYLPFMKMFGG-SFEKKVKAL 245

Query: 246 GKTMDELLQDLVDGVRNEKGEGNAMIHRLLHLQKTEPENYSDQIIKGLI 290
            + MDE+LQ L++  + +K +GN M++ LL LQ+ EPE Y+D  IKGL+
Sbjct: 246 AEAMDEILQRLLEECKRDK-DGNTMVNHLLSLQQNEPEYYTDVTIKGLM 289

[Close]

BLAST of Bhi10G001171 vs. Swiss-Prot
Match: sp|Q6WNR0|C81E7_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 1.8e-63
Identity = 130/284 (45.77%), Postives = 192/284 (67.61%), Query Frame = 0


Query: 10  LSLFSLLALRFFLQLHRQNLPPTPLFSLPIIGHLHLVKHPIHRILHNLSKKYGDVFSLRF 69
           LS+F ++ L  F     +NLPP P  SLPIIG+LH +K P++R    L++KYG+V SL F
Sbjct: 14  LSIFFIIRL-LFQSRKFKNLPPGPT-SLPIIGNLHHLKRPLNRTFKALTEKYGNVISLWF 73

Query: 70  GSRLVVVVSSPSVVQECLTKNDIILANRPLLGTGKHLAYNHTTMVVAPYGEHWRNLRRIS 129
           GSRLVVVVSS S  QEC TKND++LANRP   +GK++ YN+TT+    YGEHWRNLRRI+
Sbjct: 74  GSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRIT 133

Query: 130 ALELFSTNRLNLFSGIREDEVKGLMVRLRGSSLEEFALVEPESMFLDLMYNAIIRMVGGK 189
           +L++ S +R+N F+ IR DE + L+ +L   S  +FA VE    F D+ +N I+RM+ GK
Sbjct: 134 SLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTFNNIMRMISGK 193

Query: 190 KPCEEDKGNSR-----EFREVVTKIMEVGGATNPGDFIPIWNWIDPCGLEKKIKKLGKTM 249
           +   +D   S      +FR++V++++++ GA N  DF+P+  ++D   LEK++K++G+  
Sbjct: 194 RYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRVKRIGEKN 253

Query: 250 DELLQDLVDGVRNEKGEGNAMIHRLLHLQKTEPENYSDQIIKGL 289
           D  L  L+   R++K   N MI  LL++Q+++PE Y+D IIKGL
Sbjct: 254 DVFLSGLLQEQRSKKERTNTMIDHLLNMQESQPEYYTDTIIKGL 295

[Close]

BLAST of Bhi10G001171 vs. TrEMBL
Match: tr|A0A0A0LNA4|A0A0A0LNA4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425750 PE=3 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 1.7e-127
Identity = 233/292 (79.79%), Postives = 255/292 (87.33%), Query Frame = 0


Query: 1   MDILFLFISLSLFS-LLALRFFLQLHRQNLPPTPLFSLPIIGHLHLVKHPIHRILHNLSK 60
           MD+L L ISLSLFS LLA  F L+ HRQNLPPTP F LP+IGHLHL+KHP+HRILHNLSK
Sbjct: 1   MDVLILSISLSLFSLLLAFNFLLRPHRQNLPPTPFFCLPVIGHLHLIKHPLHRILHNLSK 60

Query: 61  KYGDVFSLRFGSRLVVVVSSPSVVQECLTKNDIILANRPLLGTGKHLAYNHTTMVVAPYG 120
            YG VFSLRFGSRLVV++SSPSVVQEC TKNDIILANRPLL TGKHLAYNHTTM VAPYG
Sbjct: 61  SYGHVFSLRFGSRLVVIISSPSVVQECFTKNDIILANRPLLDTGKHLAYNHTTMAVAPYG 120

Query: 121 EHWRNLRRISALELFSTNRLNLFSGIREDEVKGLMVRLRG-SSLEEFALVEPESMFLDLM 180
           EHWRNLRRI ALELFST+R+NLFS +RE+EVK LMVRL G SSLE F  VE ESM LDLM
Sbjct: 121 EHWRNLRRIGALELFSTSRINLFSRMREEEVKALMVRLCGSSSLEGFRAVEMESMLLDLM 180

Query: 181 YNAIIRMVGGKKPCEEDKGNSREFREVVTKIMEVGGATNPGDFIPIWNWIDPCGLEKKIK 240
           YN I+ M+GGKK CEED+G S+EFRE+VTKIM VGGA+NPGDFI IWNWIDP GL+KKI 
Sbjct: 181 YNVIMGMMGGKKGCEEDEGKSKEFREMVTKIMAVGGASNPGDFIAIWNWIDPSGLKKKIL 240

Query: 241 KLGKTMDELLQDLVDGVRNEKGEGNAMIHRLLHLQKTEPENYSDQIIKGLIQ 291
           KLG+TMD LLQ+LVDG+RNE GEGN MIHRLL LQK EPEN+SDQIIKGLIQ
Sbjct: 241 KLGQTMDVLLQELVDGMRNESGEGNTMIHRLLQLQKIEPENHSDQIIKGLIQ 292

[Close]

BLAST of Bhi10G001171 vs. TrEMBL
Match: tr|A0A0A0LQI5|A0A0A0LQI5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 2.2e-98
Identity = 187/297 (62.96%), Postives = 226/297 (76.09%), Query Frame = 0


Query: 6   LFISLSLFS-LLALRFFLQLHRQNLPPTPLFSLPIIGHLHLVKHPIHRILHNLSKKYGDV 65
           L+  LSL S LL   F  + HR NLPPTPLF LP IGHLHL+KHP+H+ L  LS+KYG V
Sbjct: 7   LYALLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTLQTLSQKYGHV 66

Query: 66  FSLRFGSRLVVVVSSPSVVQECLTKNDIILANRPLLGTGKHLAYNHTTMVVAPYGEHWRN 125
           FSL+FGSRLV++VSSPS VQEC TKNDIILANRP L +GK+L+YN+TTM V+PYGEHWRN
Sbjct: 67  FSLKFGSRLVLLVSSPSAVQECFTKNDIILANRPSLNSGKYLSYNNTTMAVSPYGEHWRN 126

Query: 126 LRRISALELFSTNRLNLFSGIREDEVKGLMVRLRGSSL--EEFALVEPESMFLDLMYNAI 185
           LRRI  LE+FST RLN FS IRE+EVK L+ +L G+    +EF +VE ESM LDL  N +
Sbjct: 127 LRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVELESMLLDLTSNIV 186

Query: 186 IRMVGGKKPCE---------EDKGNSREFREVVTKIMEVGGATNPGDFIPIWNWIDPCGL 245
           +RMVGGKK C+         ED+G  +  +E+VT+IM   G+TNPGDFIP+WNWIDP G 
Sbjct: 187 MRMVGGKKFCKENNKNINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNWIDPSGY 246

Query: 246 EKKIKKLGKTMDELLQDLVDGVRNEKGEGNAMIHRLLHLQKTEPENYSDQIIKGLIQ 291
            K+I K+G+ MDE+LQ LVD +RNE+ EGN MI  LL LQKT+P+ YSD IIKGLIQ
Sbjct: 247 NKRIMKIGRRMDEVLQRLVDEIRNEEDEGNTMIQHLLRLQKTDPKYYSDLIIKGLIQ 303

[Close]

BLAST of Bhi10G001171 vs. TrEMBL
Match: tr|A0A1S3C1S2|A0A1S3C1S2_CUCME (LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103495921 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 3.7e-98
Identity = 187/295 (63.39%), Postives = 225/295 (76.27%), Query Frame = 0


Query: 6   LFISLSLFS-LLALRFFLQLHRQNLPPTPLFSLPIIGHLHLVKHPIHRILHNLSKKYGDV 65
           L+  LSL S LL   F  + HR NLPPTPLF LP IGHLHL+KHP+H+    LS+KYG V
Sbjct: 7   LYTLLSLISLLLGYSFLFKPHRLNLPPTPLFGLPFIGHLHLLKHPVHKTFQTLSQKYGHV 66

Query: 66  FSLRFGSRLVVVVSSPSVVQECLTKNDIILANRPLLGTGKHLAYNHTTMVVAPYGEHWRN 125
           FSL+FGSRLVV+VSSPS +QEC TKNDIILANRP L +GK+LAYN+TTMVV+ YG+HWRN
Sbjct: 67  FSLKFGSRLVVLVSSPSAIQECFTKNDIILANRPSLNSGKYLAYNNTTMVVSSYGKHWRN 126

Query: 126 LRRISALELFSTNRLNLFSGIREDEVKGLMVRLRGSSL--EEFALVEPESMFLDLMYNAI 185
           LRRIS LE+FST RLN FS  RE+EVK L+ +L G+    +EF +VE E M LDL +N +
Sbjct: 127 LRRISTLEIFSTTRLNSFSRTREEEVKRLLRKLWGNYKLEDEFRVVELEPMLLDLTFNIV 186

Query: 186 IRMVGGKKPCE-------EDKGNSREFREVVTKIMEVGGATNPGDFIPIWNWIDPCGLEK 245
           +RMVGGKK CE       ED+G  + F+E+VT+IM  GG+TNPGDFIP+ NWIDP G  K
Sbjct: 187 MRMVGGKKFCEEKNNNVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWIDPSGYTK 246

Query: 246 KIKKLGKTMDELLQDLVDGVRNEKGEGNAMIHRLLHLQKTEPENYSDQIIKGLIQ 291
            I K+G+  DE+LQ LVD +RNE+ EGN MI  LL LQKT+PE YSDQIIKGL+Q
Sbjct: 247 NIMKIGRKTDEVLQGLVDEIRNEEDEGNTMIQHLLRLQKTDPEYYSDQIIKGLVQ 301

[Close]

BLAST of Bhi10G001171 vs. TrEMBL
Match: tr|A0A0A0LMZ5|A0A0A0LMZ5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423650 PE=4 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 2.4e-97
Identity = 185/296 (62.50%), Postives = 230/296 (77.70%), Query Frame = 0


Query: 1   MDILFLFISL-SLFS-LLALRFFLQLHRQNLPPTPLFSLPIIGHLHLVKHPIHRILHNLS 60
           MD+L L ISL SLFS LLA  F  Q   +NLPP+P FSLP+IGHLHL++HP+HR LHNLS
Sbjct: 1   MDLLLLSISLISLFSLLLAFNFLHQPRHKNLPPSPSFSLPLIGHLHLLRHPVHRTLHNLS 60

Query: 61  KKYGDVFSLRFGSRLVVVVSSPSVVQECLTKNDIILANRPLLGTGKHLAYNHTTMVVAPY 120
           +K G V SLRFGS L VVVSS S+VQEC TKNDIILANRPL+ +GK+L+YN+T +V++PY
Sbjct: 61  EKLGPVLSLRFGSCLAVVVSSSSLVQECFTKNDIILANRPLVKSGKYLSYNYTNLVISPY 120

Query: 121 GEHWRNLRRISALELFSTNRLNLFSGIREDEVKGLMVRL-RGSSLEEFALVEPESMFLDL 180
           GEHWRNLRRIS LE+FS +RLNLF G REDEVK L+ +L  G S EEF +VE   MF+DL
Sbjct: 121 GEHWRNLRRISTLEIFSISRLNLFVGGREDEVKRLLCKLWDGYSFEEFKVVEMFPMFMDL 180

Query: 181 MYNAIIRMVGGKKPCEE---DKGNSREFREVVTKIMEVGGATNPGDFIPIWNWIDPCGLE 240
           M+N ++RM+ GKK C +   D+  S++F+E+V +++ + GA NPGDFIP+WNWIDP    
Sbjct: 181 MFNILMRMMSGKKNCGDDLKDEERSKKFKEMVKQVLAIAGAGNPGDFIPLWNWIDPTCYN 240

Query: 241 KKIKKLGKTMDELLQDLVDGVRNEKGEGNAMIHRLLHLQKTEPENYSDQIIKGLIQ 291
           K++KKL KT DEL+Q L+D +RN+    N MI  LL LQ T+PENYSDQIIKGLIQ
Sbjct: 241 KRVKKLSKTSDELIQQLIDEIRNQNDGENTMIQHLLGLQNTQPENYSDQIIKGLIQ 296

[Close]

BLAST of Bhi10G001171 vs. TrEMBL
Match: tr|A0A1S3C323|A0A1S3C323_CUCME (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495924 PE=3 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 2.0e-96
Identity = 185/295 (62.71%), Postives = 223/295 (75.59%), Query Frame = 0


Query: 1   MDILFLFISLSLFSLL-ALRFFLQLHRQNLPPTPLFSLPIIGHLHLVKHPIHRILHNLSK 60
           MDIL L IS  L S++ A +F L+    NLPPTPLF LP+IGHLHL+KHP+H+ L NLS+
Sbjct: 1   MDILLLSISFFLLSVVFAFKFLLRPRWLNLPPTPLFWLPLIGHLHLLKHPVHQTLQNLSQ 60

Query: 61  KYGDVFSLRFGSRLVVVVSSPSVVQECLTKNDIILANRPLLGTGKHLAYNHTTMVVAPYG 120
           KYG VFSLRFGSRLVVVVSSPS VQEC TKNDIILANRPLL +GK+L YNH+++ ++PYG
Sbjct: 61  KYGHVFSLRFGSRLVVVVSSPSAVQECFTKNDIILANRPLLESGKYLGYNHSSIGLSPYG 120

Query: 121 EHWRNLRRISALELFSTNRLNLFSGIREDEVKGLMVRLRGSSL----EEFALVEPESMFL 180
           E WRNLRRI +LE+FST RLN+F GIREDEVK L+ +L G+      +EF +VE   +  
Sbjct: 121 EQWRNLRRIGSLEVFSTKRLNMFLGIREDEVKRLLRKLCGNHCKLEDDEFRVVEIGPLLK 180

Query: 181 DLMYNAIIRMVGGKKPCEEDKGNSREFREVVTKIMEVGGATNPGDFIPIWNWIDPCGLEK 240
           DL  N ++RMV GK   EED   S +FRE+VT+IME  GA+NPGDFIP+WNWIDP G  K
Sbjct: 181 DLTLNIVMRMVSGKMFYEED---SNKFREIVTQIMEHAGASNPGDFIPLWNWIDPTGFMK 240

Query: 241 KIKKLGKTMDELLQDLVDGVRNEKGEGNAMIHRLLHLQKTEPENYSDQIIKGLIQ 291
           K+KKLGKT DE LQ L+DG+RN+   GN MIH LL LQ  EPE Y+D   K +IQ
Sbjct: 241 KVKKLGKTSDEFLQQLIDGIRNQNDGGNTMIHHLLTLQNVEPEFYNDHAFKAIIQ 292

[Close]

BLAST of Bhi10G001171 vs. NCBI nr
Match: XP_023513121.1 (cytochrome P450 81D11-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 473.8 bits (1218), Expect = 4.2e-130
Identity = 234/291 (80.41%), Postives = 257/291 (88.32%), Query Frame = 0


Query: 1   MDILFLFISLSLFSL-LALRFFLQLHRQNLPPTPLFSLPIIGHLHLVKHPIHRILHNLSK 60
           MD+LFL ISLS+ S+ LA  F L+  RQNLPPTP FSLPIIGHLHL+KHP+HRILHNLSK
Sbjct: 1   MDMLFLSISLSMLSIFLASSFLLRPPRQNLPPTPFFSLPIIGHLHLIKHPLHRILHNLSK 60

Query: 61  KYGDVFSLRFGSRLVVVVSSPSVVQECLTKNDIILANRPLLGTGKHLAYNHTTMVVAPYG 120
            YGD+FSLRFGSRLVVVVSSPS+VQEC T NDI+LANRPLLGTGKHLAYNHTTM VAPYG
Sbjct: 61  TYGDIFSLRFGSRLVVVVSSPSLVQECFTMNDIVLANRPLLGTGKHLAYNHTTMAVAPYG 120

Query: 121 EHWRNLRRISALELFSTNRLNLFSGIREDEVKGLMVRLRGSSLEEFALVEPESMFLDLMY 180
           +HWRNLRRI ALE+FST+RLNLF GIREDEVK LM+RLRGSS EEF LVEPE+MFLDLMY
Sbjct: 121 DHWRNLRRIGALEIFSTSRLNLFMGIREDEVKCLMLRLRGSSSEEFTLVEPETMFLDLMY 180

Query: 181 NAIIRMVGGKKPCEEDKGNSREFREVVTKIMEVGGATNPGDFIPIWNWIDPCGLEKKIKK 240
           N I+RMV G KP E DKG SREFRE+VTKIM VGG TNPGDFIPI NWIDP GLEKKI K
Sbjct: 181 NVIMRMVAGNKPYEHDKGRSREFRELVTKIMAVGGTTNPGDFIPILNWIDPTGLEKKIMK 240

Query: 241 LGKTMDELLQDLVDGVRNEKGEGNAMIHRLLHLQKTEPENYSDQIIKGLIQ 291
           LG+ MDELLQ+LVDG+R +KG GN MIHRLLHLQK EP+N++DQIIKGLIQ
Sbjct: 241 LGQKMDELLQELVDGIRYQKGAGNTMIHRLLHLQKNEPQNHTDQIIKGLIQ 291

[Close]

BLAST of Bhi10G001171 vs. NCBI nr
Match: XP_022985605.1 (cytochrome P450 81D11-like [Cucurbita maxima])

HSP 1 Score: 472.2 bits (1214), Expect = 1.2e-129
Identity = 234/291 (80.41%), Postives = 257/291 (88.32%), Query Frame = 0


Query: 1   MDILFLFISLSLFSL-LALRFFLQLHRQNLPPTPLFSLPIIGHLHLVKHPIHRILHNLSK 60
           MDI+FL ISLS+ S+ LA  F L+  RQNLPPTP FSLPIIGHLHL++HP+HRILHNLSK
Sbjct: 1   MDIIFLSISLSMLSIFLAFIFLLRPPRQNLPPTPFFSLPIIGHLHLIRHPLHRILHNLSK 60

Query: 61  KYGDVFSLRFGSRLVVVVSSPSVVQECLTKNDIILANRPLLGTGKHLAYNHTTMVVAPYG 120
            YG VFSLRFGSR+VVVVSSPSVVQEC TKNDI+LANRPLLGTGKHLAYNHTTM VAPYG
Sbjct: 61  TYGHVFSLRFGSRIVVVVSSPSVVQECFTKNDIVLANRPLLGTGKHLAYNHTTMAVAPYG 120

Query: 121 EHWRNLRRISALELFSTNRLNLFSGIREDEVKGLMVRLRGSSLEEFALVEPESMFLDLMY 180
           +HWRNLRRI ALE+FST+RLNLF GIREDEVK LM+RLRGSS EEF LVEPE+MFLDLMY
Sbjct: 121 DHWRNLRRIGALEIFSTSRLNLFLGIREDEVKRLMLRLRGSSSEEFTLVEPETMFLDLMY 180

Query: 181 NAIIRMVGGKKPCEEDKGNSREFREVVTKIMEVGGATNPGDFIPIWNWIDPCGLEKKIKK 240
           N I+RMV G KP E DKG SREFRE+VTKIM VGG TNPGDFIPI NWIDP GLEKKI K
Sbjct: 181 NVIMRMVAGNKPYEHDKGRSREFRELVTKIMAVGGTTNPGDFIPILNWIDPTGLEKKIMK 240

Query: 241 LGKTMDELLQDLVDGVRNEKGEGNAMIHRLLHLQKTEPENYSDQIIKGLIQ 291
           LG+ MDELLQ+LVDG+R +KG GN MI RLLHLQKTEP+N++DQIIKGLIQ
Sbjct: 241 LGRKMDELLQELVDGIRYQKGAGNTMIDRLLHLQKTEPQNHTDQIIKGLIQ 291

[Close]

BLAST of Bhi10G001171 vs. NCBI nr
Match: XP_011650031.1 (PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis sativus] >KGN63298.1 hypothetical protein Csa_2G425750 [Cucumis sativus])

HSP 1 Score: 464.5 bits (1194), Expect = 2.6e-127
Identity = 233/292 (79.79%), Postives = 255/292 (87.33%), Query Frame = 0


Query: 1   MDILFLFISLSLFS-LLALRFFLQLHRQNLPPTPLFSLPIIGHLHLVKHPIHRILHNLSK 60
           MD+L L ISLSLFS LLA  F L+ HRQNLPPTP F LP+IGHLHL+KHP+HRILHNLSK
Sbjct: 1   MDVLILSISLSLFSLLLAFNFLLRPHRQNLPPTPFFCLPVIGHLHLIKHPLHRILHNLSK 60

Query: 61  KYGDVFSLRFGSRLVVVVSSPSVVQECLTKNDIILANRPLLGTGKHLAYNHTTMVVAPYG 120
            YG VFSLRFGSRLVV++SSPSVVQEC TKNDIILANRPLL TGKHLAYNHTTM VAPYG
Sbjct: 61  SYGHVFSLRFGSRLVVIISSPSVVQECFTKNDIILANRPLLDTGKHLAYNHTTMAVAPYG 120

Query: 121 EHWRNLRRISALELFSTNRLNLFSGIREDEVKGLMVRLRG-SSLEEFALVEPESMFLDLM 180
           EHWRNLRRI ALELFST+R+NLFS +RE+EVK LMVRL G SSLE F  VE ESM LDLM
Sbjct: 121 EHWRNLRRIGALELFSTSRINLFSRMREEEVKALMVRLCGSSSLEGFRAVEMESMLLDLM 180

Query: 181 YNAIIRMVGGKKPCEEDKGNSREFREVVTKIMEVGGATNPGDFIPIWNWIDPCGLEKKIK 240
           YN I+ M+GGKK CEED+G S+EFRE+VTKIM VGGA+NPGDFI IWNWIDP GL+KKI 
Sbjct: 181 YNVIMGMMGGKKGCEEDEGKSKEFREMVTKIMAVGGASNPGDFIAIWNWIDPSGLKKKIL 240

Query: 241 KLGKTMDELLQDLVDGVRNEKGEGNAMIHRLLHLQKTEPENYSDQIIKGLIQ 291
           KLG+TMD LLQ+LVDG+RNE GEGN MIHRLL LQK EPEN+SDQIIKGLIQ
Sbjct: 241 KLGQTMDVLLQELVDGMRNESGEGNTMIHRLLQLQKIEPENHSDQIIKGLIQ 292

[Close]

BLAST of Bhi10G001171 vs. NCBI nr
Match: XP_022149516.1 (cytochrome P450 81E8-like [Momordica charantia])

HSP 1 Score: 404.8 bits (1039), Expect = 2.4e-109
Identity = 210/292 (71.92%), Postives = 233/292 (79.79%), Query Frame = 0


Query: 1   MDILFLFISLSLFSLLALRFFLQLHRQNLPPTPLFSLPIIGHLHLVKHPIHRILHNLSKK 60
           MDIL L I LSL  LLA   F    R NLPP+PLFSLPIIGHLHLVKH +HR LHNLS  
Sbjct: 1   MDILLLSIFLSL--LLAFLLFRPHRRNNLPPSPLFSLPIIGHLHLVKHSVHRTLHNLSHN 60

Query: 61  YGDVFSLRFGSRLVVVVSSPSVVQECLTKNDIILANRPLLGTGKHLAYNHTTMVVAPYGE 120
           YG VFSLRFGSRLVVVVSSPSVVQEC TKNDIILANRPLL T KHLAYN TTM VAPYG+
Sbjct: 61  YGRVFSLRFGSRLVVVVSSPSVVQECFTKNDIILANRPLLDTAKHLAYNQTTMAVAPYGD 120

Query: 121 HWRNLRRISALELFSTNRLNLFSGIREDEVKGLMVRLRGSSLEEFALVEPESMFLDLMYN 180
           HWR+LRRI ALE+FST R+NLF GIRE EVK LM +L GSS  EF +VEP SM LDLM+N
Sbjct: 121 HWRHLRRIGALEIFSTTRMNLFLGIREGEVKRLMRKLCGSSFVEFTVVEPGSMILDLMFN 180

Query: 181 AIIRMVGGKKPCEED---KGNSREFREVVTKIMEVGGATNPGDFIPIWNWIDPCGLEKKI 240
            I+R+V GKK  ++D    G SR+FRE V + M  GGATNPGDFIPIWNWIDP GLEKK+
Sbjct: 181 IIMRIVAGKKYYDDDMSNDGKSRKFREAVKETMAHGGATNPGDFIPIWNWIDPTGLEKKM 240

Query: 241 KKLGKTMDELLQDLVDGVRNEKGEGNAMIHRLLHLQKTEPENYSDQIIKGLI 290
            KLG TMDELLQ+L+D +RN+K EGN MI  LL LQK+EPEN+SD IIKGL+
Sbjct: 241 MKLGHTMDELLQELLDEIRNQKNEGNTMIEHLLCLQKSEPENHSDLIIKGLV 290

[Close]

BLAST of Bhi10G001171 vs. NCBI nr
Match: XP_022149455.1 (cytochrome P450 81E8-like [Momordica charantia])

HSP 1 Score: 401.4 bits (1030), Expect = 2.7e-108
Identity = 203/291 (69.76%), Postives = 234/291 (80.41%), Query Frame = 0


Query: 1   MDILFLFISLSLFS-LLALRFFLQLHRQNLPPTPLFSLPIIGHLHLVKHPIHRILHNLSK 60
           MDILFL ISLSLFS LLA  F  +  RQNLPP+PLFSLPI+GHLHLVKHP+H+  H LS 
Sbjct: 1   MDILFLSISLSLFSLLLAFSFLFRSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSH 60

Query: 61  KYGDVFSLRFGSRLVVVVSSPSVVQECLTKNDIILANRPLLGTGKHLAYNHTTMVVAPYG 120
           KYG VFSLRFGSR+VVVVSSPS VQEC TKNDI+LANRPLL +GK LAYNHTTM VAPYG
Sbjct: 61  KYGHVFSLRFGSRVVVVVSSPSAVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYG 120

Query: 121 EHWRNLRRISALELFSTNRLNLFSGIREDEVKGLMVRLRGSSLEEFALVEPESMFLDLMY 180
           EHWRNLRRI  LE+FST RLN+F GIREDEV+ LM++L  +S EEF  VE E M  DL +
Sbjct: 121 EHWRNLRRIGTLEIFSTTRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTF 180

Query: 181 NAIIRMVGGKKPCEEDKGNSREFREVVTKIMEVGGATNPGDFIPIWNWIDPCGLEKKIKK 240
           N ++RMV GKK  EE +  S++FRE+VT+++   GATNPGDFIPIWNWIDP G EK+I K
Sbjct: 181 NIVMRMVAGKKYYEEQQ--SKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIK 240

Query: 241 LGKTMDELLQDLVDGVRNEKGEGNAMIHRLLHLQKTEPENYSDQIIKGLIQ 291
           LG+  DELLQ+L+D +RN++G  N MI  LL LQK EPE YSDQIIKGLIQ
Sbjct: 241 LGQRSDELLQELIDEIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQ 289

The following BLAST results are available for this feature:

BLAST of Bhi10G001171 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G37310.1	9.9e-73	50.68	cytochrome P450, family 81, subfamily H, polypeptide 1	[more]
AT4G37320.1	1.0e-69	46.21	cytochrome P450, family 81, subfamily D, polypeptide 5	[more]
AT2G23190.1	9.6e-68	46.80	cytochrome P450, family 81, subfamily D, polypeptide 7	[more]
AT4G37330.1	3.6e-67	47.28	cytochrome P450, family 81, subfamily D, polypeptide 4	[more]
AT4G37340.1	2.4e-66	48.45	cytochrome P450, family 81, subfamily D, polypeptide 3	[more]

BLAST of Bhi10G001171 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q6WNQ8\|C81E8_MEDTR	2.6e-70	48.48	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
sp\|P93147\|C81E1_GLYEC	7.7e-67	47.81	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
sp\|Q6WNQ9\|C81E9_MEDTR	6.6e-66	45.00	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
sp\|Q0WTF4\|C81F3_ARATH	4.7e-64	46.02	Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1	[more]
sp\|Q6WNR0\|C81E7_MEDTR	1.8e-63	45.77	Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1	[more]

BLAST of Bhi10G001171 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LNA4\|A0A0A0LNA4_CUCSA	1.7e-127	79.79	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425750 PE=3 SV=1	[more]
tr\|A0A0A0LQI5\|A0A0A0LQI5_CUCSA	2.2e-98	62.96	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1	[more]
tr\|A0A1S3C1S2\|A0A1S3C1S2_CUCME	3.7e-98	63.39	LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=L...	[more]
tr\|A0A0A0LMZ5\|A0A0A0LMZ5_CUCSA	2.4e-97	62.50	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423650 PE=4 SV=1	[more]
tr\|A0A1S3C323\|A0A1S3C323_CUCME	2.0e-96	62.71	cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495924 PE=3 SV=1	[more]

BLAST of Bhi10G001171 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023513121.1	4.2e-130	80.41	cytochrome P450 81D11-like [Cucurbita pepo subsp. pepo]	[more]
XP_022985605.1	1.2e-129	80.41	cytochrome P450 81D11-like [Cucurbita maxima]	[more]
XP_011650031.1	2.6e-127	79.79	PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis sativus] >KGN63298.1 hypothet...	[more]
XP_022149516.1	2.4e-109	71.92	cytochrome P450 81E8-like [Momordica charantia]	[more]
XP_022149455.1	2.7e-108	69.76	cytochrome P450 81E8-like [Momordica charantia]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037	heme binding
GO:0005506	iron ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450
IPR036396	Cyt_P450_sf
IPR002401	Cyt_P450_E_grp-I

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0016020	membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi10M001171	Bhi10M001171	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 82..103 score: 23.31 coord: 177..195 score: 23.1 coord: 58..77 score: 47.17
IPR036396	Cytochrome P450 superfamily	GENE3D	G3DSA:1.10.630.10		coord: 22..290 e-value: 1.6E-47 score: 164.4
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	SSF48264	Cytochrome P450	coord: 30..288
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 30..283 e-value: 3.3E-25 score: 88.7
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 10..289
None	No IPR available	PANTHER	PTHR24298:SF205	CYTOCHROME P450 81D1-RELATED	coord: 10..289

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi10G001171	Cucumber (Gy14) v1	cgywgoB076
Bhi10G001171	Cucumber (Gy14) v2	cgybwgoB168
Bhi10G001171	Cucurbita maxima (Rimu)	cmawgoB0696
Bhi10G001171	Cucurbita maxima (Rimu)	cmawgoB0759
Bhi10G001171	Cucurbita moschata (Rifu)	cmowgoB0690
Bhi10G001171	Cucurbita moschata (Rifu)	cmowgoB0749
Bhi10G001171	Cucurbita pepo (Zucchini)	cpewgoB0358
Bhi10G001171	Cucurbita pepo (Zucchini)	cpewgoB0950
Bhi10G001171	Wild cucumber (PI 183967)	cpiwgoB171
Bhi10G001171	Cucumber (Chinese Long) v3	cucwgoB180
Bhi10G001171	Cucumber (Chinese Long) v2	cuwgoB174
Bhi10G001171	Bottle gourd (USVL1VR-Ls)	lsiwgoB069
Bhi10G001171	Melon (DHL92) v3.6.1	medwgoB128
Bhi10G001171	Melon (DHL92) v3.5.1	mewgoB126
Bhi10G001171	Watermelon (Charleston Gray)	wcgwgoB299
Bhi10G001171	Watermelon (97103) v2	wgowmbB370
Bhi10G001171	Watermelon (97103) v1	wgowmB382
Bhi10G001171	Silver-seed gourd	carwgoB0497
Bhi10G001171	Silver-seed gourd	carwgoB1106