Bhi10G001167 (gene) Wax gourd

Overview
NameBhi10G001167
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionCytochrome P450
Locationchr10 : 35903057 .. 35903442 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGTGTAAAATAGAAGGATATGATGTTCCATGTAATACAATTATATTGATCAATGCTTGGGCTGTTCATAGAGATTCAAGTATTTGGGAAGATGCCGCAAGCTTTAAACCTGAAAGACATGCAAGTGGGATTGAAGTTGATTATTCATATAAACTAATACCATTTGGAAGTGGGAGGAGAGCATGTCCTGGTGTAGGAATGGCTCAACGAGTGGCTGGTTTGACTCTAGCATCATCGATACAAAGCTTTGAATGGGAAAGAGTGAACAACTCATTAATTGACATGAGTGAAGGTAAAGGACTCACAATGCCTAAAGCTCAACCATTGGTAGCTAAGTGTAAACCACGTCTAATCATGAAAGTCATTTTGAACGCACGTAGCAATTGA

mRNA sequence

AGTGTAAAATAGAAGGATATGATGTTCCATGTAATACAATTATATTGATCAATGCTTGGGCTGTTCATAGAGATTCAAGTATTTGGGAAGATGCCGCAAGCTTTAAACCTGAAAGACATGCAAGTGGGATTGAAGTTGATTATTCATATAAACTAATACCATTTGGAAGTGGGAGGAGAGCATGTCCTGGTGTAGGAATGGCTCAACGAGTGGCTGGTTTGACTCTAGCATCATCGATACAAAGCTTTGAATGGGAAAGAGTGAACAACTCATTAATTGACATGAGTGAAGGTAAAGGACTCACAATGCCTAAAGCTCAACCATTGGTAGCTAAGTGTAAACCACGTCTAATCATGAAAGTCATTTTGAACGCACGTAGCAATTGA

Coding sequence (CDS)

AGTGTAAAATAGAAGGATATGATGTTCCATGTAATACAATTATATTGATCAATGCTTGGGCTGTTCATAGAGATTCAAGTATTTGGGAAGATGCCGCAAGCTTTAAACCTGAAAGACATGCAAGTGGGATTGAAGTTGATTATTCATATAAACTAATACCATTTGGAAGTGGGAGGAGAGCATGTCCTGGTGTAGGAATGGCTCAACGAGTGGCTGGTTTGACTCTAGCATCATCGATACAAAGCTTTGAATGGGAAAGAGTGAACAACTCATTAATTGACATGAGTGAAGGTAAAGGACTCACAATGCCTAAAGCTCAACCATTGGTAGCTAAGTGTAAACCACGTCTAATCATGAAAGTCATTTTGAACGCACGTAGCAATTGA

Protein sequence

CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRACPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKVILNARSN
Homology
[Close]
BLAST of Bhi10G001167 vs. TAIR10
Match: AT1G66540.1 (Cytochrome P450 superfamily protein)

HSP 1 Score: 148.7 bits (374), Expect = 2.5e-36
Identity = 65/115 (56.52%), Postives = 85/115 (73.91%), Query Frame = 0 

Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           CK+ GYD+PC T++L+N WA+HRD  +W+D ASFKPER     E   ++KL+ FG GRRA
Sbjct: 264 CKVGGYDMPCGTMLLVNVWAIHRDPRLWDDPASFKPERFEKEGE---THKLLTFGLGRRA 323

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPR 116
           CPG G+A+R+  L+L S IQ FEWER+    +DM+EG GLTMP+A PLVA C+ R
Sbjct: 324 CPGSGLARRLVSLSLGSLIQCFEWERIGEEEVDMTEGGGLTMPRAIPLVAMCRAR 375

[Close]
BLAST of Bhi10G001167 vs. TAIR10
Match: AT4G37430.1 (cytochrome P450, family 91, subfamily A, polypeptide 2)

HSP 1 Score: 144.8 bits (364), Expect = 3.6e-35
Identity = 67/122 (54.92%), Postives = 82/122 (67.21%), Query Frame = 0 

Query: 2   KIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVD-YSYKLIPFGSGRRA 61
           KI GYDVP +TI+++NAWA+HRD  IWE+   F P+R+  G   D Y YKL+PFG+GRR 
Sbjct: 378 KIGGYDVPRDTIVMVNAWAIHRDPEIWEEPEKFNPDRYNDGCGSDYYVYKLMPFGNGRRT 437

Query: 62  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 121
           CPG G+ QR+  L L S IQ FEWE V    +DMSE  GL M K  PL A C+PR IM  
Sbjct: 438 CPGAGLGQRIVTLALGSLIQCFEWENVKGEEMDMSESTGLGMRKMDPLRAMCRPRPIMSK 497

Query: 122 IL 123
           +L
Sbjct: 498 LL 499

[Close]
BLAST of Bhi10G001167 vs. TAIR10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2)

HSP 1 Score: 144.4 bits (363), Expect = 4.7e-35
Identity = 68/122 (55.74%), Postives = 86/122 (70.49%), Query Frame = 0 

Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           CK+ GYD+P  T++L NAWA+HRD  +W+D  SFKPER     E   + KL+PFG GRRA
Sbjct: 377 CKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEKEGE---AKKLMPFGLGRRA 436

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 120
           CPG G+AQR+  L+L S IQ FEWER+    +DM+EG GLTMPKA+PL A C+ R  +  
Sbjct: 437 CPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKARPLEAMCRARDFVGK 495

Query: 121 IL 123
           IL
Sbjct: 497 IL 495

[Close]
BLAST of Bhi10G001167 vs. TAIR10
Match: AT5G67310.1 (cytochrome P450, family 81, subfamily G, polypeptide 1)

HSP 1 Score: 141.7 bits (356), Expect = 3.0e-34
Identity = 67/123 (54.47%), Postives = 88/123 (71.54%), Query Frame = 0 

Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           C++ G+D+P  T + INAWA+ RD ++W+D  +FKPER  S     +  K +PFG GRRA
Sbjct: 388 CEVAGFDIPRRTWLFINAWAIQRDPNVWDDPETFKPERFES---ETHRGKFLPFGIGRRA 447

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIM-K 120
           CPG+G+AQ V  L L S IQ F+WER N+  +DMSEGKGLTMPK+ PLVAKCK   I+ K
Sbjct: 448 CPGMGLAQLVLSLALGSLIQCFDWERDNDVAVDMSEGKGLTMPKSVPLVAKCKSLPILDK 507

Query: 121 VIL 123
           ++L
Sbjct: 508 LVL 507

[Close]
BLAST of Bhi10G001167 vs. TAIR10
Match: AT4G37310.1 (cytochrome P450, family 81, subfamily H, polypeptide 1)

HSP 1 Score: 141.4 bits (355), Expect = 4.0e-34
Identity = 63/123 (51.22%), Postives = 86/123 (69.92%), Query Frame = 0 

Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEV-DYSYKLIPFGSGRR 60
           C I G++VP +TI+L+N WA+HRD S+W+D  SFKPER     +   Y+ K++PFG GRR
Sbjct: 385 CVIGGFNVPRDTIVLVNLWAIHRDPSVWDDPTSFKPERFEGSDQFGHYNGKMMPFGLGRR 444

Query: 61  ACPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMK 120
           ACPG+ +A RV GL L S IQ FEWE  +   +DM+EG GL++PKA+PLV  C+ R +  
Sbjct: 445 ACPGLSLANRVVGLLLGSMIQCFEWESGSGGQVDMTEGPGLSLPKAEPLVVTCRTREMAS 504

Query: 121 VIL 123
            +L
Sbjct: 505 ELL 507

[Close]
BLAST of Bhi10G001167 vs. Swiss-Prot
Match: sp|O65790|C81F1_ARATH (Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2)

HSP 1 Score: 144.8 bits (364), Expect = 6.5e-34
Identity = 67/122 (54.92%), Postives = 82/122 (67.21%), Query Frame = 0 

Query: 2   KIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVD-YSYKLIPFGSGRRA 61
           KI GYDVP +TI+++NAWA+HRD  IWE+   F P+R+  G   D Y YKL+PFG+GRR 
Sbjct: 378 KIGGYDVPRDTIVMVNAWAIHRDPEIWEEPEKFNPDRYNDGCGSDYYVYKLMPFGNGRRT 437

Query: 62  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 121
           CPG G+ QR+  L L S IQ FEWE V    +DMSE  GL M K  PL A C+PR IM  
Sbjct: 438 CPGAGLGQRIVTLALGSLIQCFEWENVKGEEMDMSESTGLGMRKMDPLRAMCRPRPIMSK 497

Query: 122 IL 123
           +L
Sbjct: 498 LL 499

[Close]
BLAST of Bhi10G001167 vs. Swiss-Prot
Match: sp|Q9FG65|C81D1_ARATH (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 1.2e-32
Identity = 68/123 (55.28%), Postives = 83/123 (67.48%), Query Frame = 0 

Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           CKI  YD+P  T +L+NAWA+HRD + W+D  SFKPER     E   + KL+ FG GRRA
Sbjct: 383 CKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKEEE---AQKLLAFGLGRRA 442

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 120
           CPG G+AQR+ GL L S IQ FEWERV N  +DM EG G T+PKA PL A CK R  +  
Sbjct: 443 CPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKAICKARPFLHK 502

Query: 121 ILN 124
           I++
Sbjct: 503 IIS 502

[Close]
BLAST of Bhi10G001167 vs. Swiss-Prot
Match: sp|Q9LHA1|C8D11_ARATH (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 6.7e-31
Identity = 67/125 (53.60%), Postives = 82/125 (65.60%), Query Frame = 0 

Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           C ++GYDVP  TIIL+NAWA+HRD  +WE+   FKPER     E     KL+PFG GRR+
Sbjct: 385 CIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEKKGE---DKKLMPFGIGRRS 444

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEG-KGLTMPKAQPLVAKCKPRLIMK 120
           CPG G+AQR+  L L S +Q FEWERV    +DM E  KG TM KA  L A CK R I+ 
Sbjct: 445 CPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTMRKATSLQAMCKARPIVH 504

Query: 121 VILNA 125
            +L+A
Sbjct: 505 KVLDA 506

[Close]
BLAST of Bhi10G001167 vs. Swiss-Prot
Match: sp|Q6WNR0|C81E7_MEDTR (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 2.1e-29
Identity = 61/121 (50.41%), Postives = 80/121 (66.12%), Query Frame = 0 

Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           C + GY VP +TI+LINAWA+HRD   W +A +FKPER     E++   K+I FG GRRA
Sbjct: 379 CIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFDKKGELE---KMIAFGMGRRA 438

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 120
           CPG G+A R   +TLA  +Q F+W+R+N+  IDMSE  G TM K  PL A CK R ++  
Sbjct: 439 CPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDGFTMTKLLPLKAMCKTRPVVNK 496

Query: 121 I 122
           +
Sbjct: 499 V 496

[Close]
BLAST of Bhi10G001167 vs. Swiss-Prot
Match: sp|Q9SZU1|C81F4_ARATH (Cytochrome P450 81F4 OS=Arabidopsis thaliana OX=3702 GN=CYP81F4 PE=1 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 4.8e-29
Identity = 62/118 (52.54%), Postives = 77/118 (65.25%), Query Frame = 0 

Query: 2   KIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGI-EVDYSYKLIPFGSGRRA 61
           KI GYDVP +T++++NAWA+HRD  +W +   F PER   G  E D    LI FGSGRR 
Sbjct: 375 KIGGYDVPRDTMVMVNAWAIHRDPDLWTEPERFNPERFNGGEGEKDDVRMLIAFGSGRRI 434

Query: 62  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIM 119
           CPGVG+A ++  L L S IQ F+W++VN   IDMSEG G+ M    PL A CK R IM
Sbjct: 435 CPGVGLAHKIVTLALGSLIQCFDWKKVNEKEIDMSEGPGMAMRMMVPLRALCKTRPIM 492

[Close]
BLAST of Bhi10G001167 vs. TrEMBL
Match: tr|A0A0A0LTD0|A0A0A0LTD0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425740 PE=3 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 4.5e-48
Identity = 89/123 (72.36%), Postives = 107/123 (86.99%), Query Frame = 0 

Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           C+IEGYD+P  TII +NAWA+HRDSS+WED  SFKPERH + IE+  SYKL+PFG GRRA
Sbjct: 380 CQIEGYDIPRGTIIFVNAWAIHRDSSLWEDVTSFKPERHENAIELSDSYKLLPFGLGRRA 439

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 120
           CPGVGMAQRV GLTLAS IQ F+WER+++SL+DM+EG+G+TMPKAQPLVAKCKPR IM+ 
Sbjct: 440 CPGVGMAQRVLGLTLASLIQCFDWERMDSSLVDMTEGQGITMPKAQPLVAKCKPRPIMRA 499

Query: 121 ILN 124
            L+
Sbjct: 500 HLS 502

[Close]
BLAST of Bhi10G001167 vs. TrEMBL
Match: tr|A0A0A0LQI5|A0A0A0LQI5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.5e-46
Identity = 85/126 (67.46%), Postives = 105/126 (83.33%), Query Frame = 0 

Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           CKI GYDVP NTI+LINAWA+HRD ++WEDA SFKPERH + +  + SYKL+PFG GRRA
Sbjct: 385 CKIGGYDVPRNTIVLINAWAIHRDPNVWEDAGSFKPERHVNAVGFENSYKLLPFGLGRRA 444

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 120
           CPG+ MAQRV GLTLAS IQ FEW++++N L+DM EG+GLTMPK + LVAKC+PR IMKV
Sbjct: 445 CPGMAMAQRVVGLTLASLIQCFEWKKMSNLLVDMREGEGLTMPKVESLVAKCRPRFIMKV 504

Query: 121 ILNARS 127
           +L  ++
Sbjct: 505 VLGEKN 510

[Close]
BLAST of Bhi10G001167 vs. TrEMBL
Match: tr|A0A1S3C2H7|A0A1S3C2H7_CUCME (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495922 PE=3 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 7.1e-46
Identity = 89/125 (71.20%), Postives = 105/125 (84.00%), Query Frame = 0 

Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           CKI GYDVP +TI+LINAW +HRD S+WEDA SFKPERH +   VD +YKL+PFG GRRA
Sbjct: 379 CKIGGYDVPRDTIVLINAWTIHRDPSLWEDATSFKPERHENANGVD-AYKLLPFGLGRRA 438

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 120
           CPG+GMAQRV  LTLAS IQ FEW+R+ NSL+DM+EG+GLTMPKAQPL AKC+PR IMK 
Sbjct: 439 CPGMGMAQRVVALTLASLIQCFEWQRLGNSLVDMTEGEGLTMPKAQPLTAKCRPRPIMKK 498

Query: 121 ILNAR 126
           IL+ +
Sbjct: 499 ILSMK 502

[Close]
BLAST of Bhi10G001167 vs. TrEMBL
Match: tr|A0A1S3C312|A0A1S3C312_CUCME (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495915 PE=3 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.6e-45
Identity = 86/123 (69.92%), Postives = 106/123 (86.18%), Query Frame = 0 

Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           CKI GYDVP +TI++INAWA+HRD ++WEDA+SFKPERH + I V+ SYK IPFG GRRA
Sbjct: 261 CKIGGYDVPRDTIVIINAWAIHRDPNLWEDASSFKPERHTNPIGVE-SYKFIPFGLGRRA 320

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 120
           CPG+G+AQ++A LTLA+ IQ FEWER  +SL+DMSEG+GLTMPKAQPL+AKCKPR IMK 
Sbjct: 321 CPGIGIAQKMANLTLATMIQCFEWEREGSSLVDMSEGEGLTMPKAQPLIAKCKPRPIMKA 380

Query: 121 ILN 124
           + +
Sbjct: 381 VFS 382

[Close]
BLAST of Bhi10G001167 vs. TrEMBL
Match: tr|A0A0A0LTB7|A0A0A0LTB7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423660 PE=3 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 3.5e-45
Identity = 88/125 (70.40%), Postives = 104/125 (83.20%), Query Frame = 0 

Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           CKI GYDVP +T +LINAWA+HRD S+WEDA SFKPERH +   VD +YKL+PFG GRRA
Sbjct: 93  CKIGGYDVPRDTTVLINAWAIHRDPSLWEDATSFKPERHENANGVD-AYKLLPFGLGRRA 152

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 120
           CPGVGMAQRV  LTLAS +Q FEW+R+ NSL+DM+EG+GLTMPKAQPL AKC+PR IM  
Sbjct: 153 CPGVGMAQRVVALTLASLLQCFEWQRLGNSLVDMTEGEGLTMPKAQPLTAKCRPRPIMMK 212

Query: 121 ILNAR 126
           IL+ +
Sbjct: 213 ILSMK 216

[Close]
BLAST of Bhi10G001167 vs. NCBI nr
Match: XP_023512186.1 (isoflavone 2'-hydroxylase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023512187.1 isoflavone 2'-hydroxylase-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 209.5 bits (532), Expect = 6.5e-51
Identity = 99/122 (81.15%), Postives = 111/122 (90.98%), Query Frame = 0 

Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDY-SYKLIPFGSGRR 60
           CKIEGYDVP +TIILINAWA+HRD ++WEDA SFKPERHA+G++    SYKLIPFG GRR
Sbjct: 370 CKIEGYDVPRDTIILINAWAMHRDPNLWEDATSFKPERHANGMKNGADSYKLIPFGLGRR 429

Query: 61  ACPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMK 120
           ACPGVGMAQRVAGLTLAS IQ FEWER+NNSL+DMSEGKG+TMPKAQPLVAKCKPR IMK
Sbjct: 430 ACPGVGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVAKCKPRPIMK 489

Query: 121 VI 122
           ++
Sbjct: 490 LV 491

[Close]
BLAST of Bhi10G001167 vs. NCBI nr
Match: XP_022985609.1 (isoflavone 2'-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 208.4 bits (529), Expect = 1.5e-50
Identity = 98/122 (80.33%), Postives = 111/122 (90.98%), Query Frame = 0 

Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVD-YSYKLIPFGSGRR 60
           CKIEGYDVP +TIILINAWA+HRD ++WEDA SFKPERHA+G++    SYKLIPFG GRR
Sbjct: 371 CKIEGYDVPRDTIILINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYKLIPFGLGRR 430

Query: 61  ACPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMK 120
           ACPG+GMAQRVAGLTLAS IQ FEWER+NNSL+DMSEGKG+TMPKAQPLVAKCKPR IMK
Sbjct: 431 ACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVAKCKPRPIMK 490

Query: 121 VI 122
           ++
Sbjct: 491 LV 492

[Close]
BLAST of Bhi10G001167 vs. NCBI nr
Match: XP_022943410.1 (isoflavone 2'-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 206.8 bits (525), Expect = 4.2e-50
Identity = 97/122 (79.51%), Postives = 110/122 (90.16%), Query Frame = 0 

Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVD-YSYKLIPFGSGRR 60
           CKIEGYDVP +TII INAWA+HRD ++WEDA SFKPERHA+G++    SYKLIPFG GRR
Sbjct: 371 CKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYKLIPFGLGRR 430

Query: 61  ACPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMK 120
           ACPG+GMAQRVAGLTLAS IQ FEWER+NNSL+DMSEGKG+TMPKAQPLVAKCKPR IMK
Sbjct: 431 ACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVAKCKPRPIMK 490

Query: 121 VI 122
           ++
Sbjct: 491 LV 492

[Close]
BLAST of Bhi10G001167 vs. NCBI nr
Match: XP_022943757.1 (isoflavone 2'-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 206.8 bits (525), Expect = 4.2e-50
Identity = 97/122 (79.51%), Postives = 110/122 (90.16%), Query Frame = 0 

Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVD-YSYKLIPFGSGRR 60
           CKIEGYDVP +TII INAWA+HRD ++WEDA SFKPERHA+G++    SYKLIPFG GRR
Sbjct: 370 CKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYKLIPFGLGRR 429

Query: 61  ACPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMK 120
           ACPG+GMAQRVAGLTLAS IQ FEWER+NNSL+DMSEGKG+TMPKAQPLVAKCKPR IMK
Sbjct: 430 ACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVAKCKPRPIMK 489

Query: 121 VI 122
           ++
Sbjct: 490 LV 491

[Close]
BLAST of Bhi10G001167 vs. NCBI nr
Match: XP_022149460.1 (isoflavone 2'-hydroxylase-like [Momordica charantia])

HSP 1 Score: 205.7 bits (522), Expect = 9.4e-50
Identity = 96/122 (78.69%), Postives = 109/122 (89.34%), Query Frame = 0 

Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           CKIEGYD+P +TIILINAWA+HRDS+IWEDA SFKPERHA+ +    SYKL+PFG GRRA
Sbjct: 456 CKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGGVDSYKLLPFGLGRRA 515

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 120
           CPG+GMAQRV GLTLAS IQ FEWERVN+SL+DM+EG+GLTMPKAQPLVAKCKPR I+K 
Sbjct: 516 CPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKAQPLVAKCKPRPILKA 575

Query: 121 IL 123
           IL
Sbjct: 576 IL 577

The following BLAST results are available for this feature:
BLAST of Bhi10G001167 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G66540.12.5e-3656.52Cytochrome P450 superfamily protein[more]
AT4G37430.13.6e-3554.92cytochrome P450, family 91, subfamily A, polypeptide 2[more]
AT4G37360.14.7e-3555.74cytochrome P450, family 81, subfamily D, polypeptide 2[more]
AT5G67310.13.0e-3454.47cytochrome P450, family 81, subfamily G, polypeptide 1[more]
AT4G37310.14.0e-3451.22cytochrome P450, family 81, subfamily H, polypeptide 1[more]
BLAST of Bhi10G001167 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|O65790|C81F1_ARATH6.5e-3454.92Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2[more]
sp|Q9FG65|C81D1_ARATH1.2e-3255.28Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1[more]
sp|Q9LHA1|C8D11_ARATH6.7e-3153.60Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1[more]
sp|Q6WNR0|C81E7_MEDTR2.1e-2950.41Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1[more]
sp|Q9SZU1|C81F4_ARATH4.8e-2952.54Cytochrome P450 81F4 OS=Arabidopsis thaliana OX=3702 GN=CYP81F4 PE=1 SV=1[more]
BLAST of Bhi10G001167 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0LTD0|A0A0A0LTD0_CUCSA4.5e-4872.36Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425740 PE=3 SV=1[more]
tr|A0A0A0LQI5|A0A0A0LQI5_CUCSA2.5e-4667.46Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1[more]
tr|A0A1S3C2H7|A0A1S3C2H7_CUCME7.1e-4671.20cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495922 PE=3 SV=1[more]
tr|A0A1S3C312|A0A1S3C312_CUCME1.6e-4569.92cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495915 PE=3 SV=1[more]
tr|A0A0A0LTB7|A0A0A0LTB7_CUCSA3.5e-4570.40Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423660 PE=3 SV=1[more]
BLAST of Bhi10G001167 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023512186.16.5e-5181.15isoflavone 2'-hydroxylase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_02351... [more]
XP_022985609.11.5e-5080.33isoflavone 2'-hydroxylase-like [Cucurbita maxima][more]
XP_022943410.14.2e-5079.51isoflavone 2'-hydroxylase-like [Cucurbita moschata][more]
XP_022943757.14.2e-5079.51isoflavone 2'-hydroxylase-like [Cucurbita moschata][more]
XP_022149460.19.4e-5078.69isoflavone 2'-hydroxylase-like [Momordica charantia][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037heme binding
GO:0005506iron ion binding
Vocabulary: INTERPRO
TermDefinition
IPR017972Cyt_P450_CS
IPR036396Cyt_P450_sf
IPR001128Cyt_P450
IPR002401Cyt_P450_E_grp-I
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi10M001167Bhi10M001167mRNA


InterPro
Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 123..127
NoneNo IPR availablePANTHERPTHR24298FAMILY NOT NAMEDcoord: 1..122
NoneNo IPR availablePANTHERPTHR24298:SF205CYTOCHROME P450 81D1-RELATEDcoord: 1..122
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 51..61
score: 54.73
coord: 17..41
score: 29.85
coord: 61..84
score: 30.65
IPR001128Cytochrome P450PFAMPF00067p450coord: 1..105
e-value: 3.6E-25
score: 88.6
IPR036396Cytochrome P450 superfamilyGENE3DG3DSA:1.10.630.10coord: 1..119
e-value: 3.2E-33
score: 117.2
IPR036396Cytochrome P450 superfamilySUPERFAMILYSSF48264Cytochrome P450coord: 1..115
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 54..63

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Bhi10G001167Cucumber (Gy14) v1cgywgoB076
Bhi10G001167Cucumber (Gy14) v2cgybwgoB168
Bhi10G001167Cucurbita maxima (Rimu)cmawgoB0696
Bhi10G001167Cucurbita maxima (Rimu)cmawgoB0759
Bhi10G001167Cucurbita moschata (Rifu)cmowgoB0690
Bhi10G001167Cucurbita moschata (Rifu)cmowgoB0749
Bhi10G001167Cucurbita pepo (Zucchini)cpewgoB0358
Bhi10G001167Cucurbita pepo (Zucchini)cpewgoB0950
Bhi10G001167Wild cucumber (PI 183967)cpiwgoB171
Bhi10G001167Cucumber (Chinese Long) v3cucwgoB180
Bhi10G001167Cucumber (Chinese Long) v2cuwgoB174
Bhi10G001167Bottle gourd (USVL1VR-Ls)lsiwgoB069
Bhi10G001167Melon (DHL92) v3.6.1medwgoB128
Bhi10G001167Melon (DHL92) v3.5.1mewgoB126
Bhi10G001167Watermelon (Charleston Gray)wcgwgoB299
Bhi10G001167Watermelon (97103) v2wgowmbB370
Bhi10G001167Silver-seed gourdcarwgoB0497
Bhi10G001167Silver-seed gourdcarwgoB1106