Bhi10G000619 (gene) Wax gourd

NameBhi10G000619
Typegene
OrganismBenincasa hispida (Wax gourd)
Descriptionjosephin-like protein
Locationchr10 : 16196749 .. 16197581 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAGCGAGGATTGCCGAGTTTATCACGAGAGACAAAAGCTACAGTTCTGTCTCTTGCACGCTCTCAATAACCTATTTCAGGTATCTTTTCATCCTCAAGCCTCTGACTGTGGAAGTTTTCCAACCGTTTTCTTATTGACTGATATTGAAAAGTCGTTCAACCTTGAAATTTATTATTTGCTTTGTAATTAAGATGCTGATTAACGGAATTATACTGTTTCTCATCATTAAGAAAGAACTATGGCACATAGGATTTCAGAATTCGAACTTATCATCTCACTGTTTCACGATTTGTTGCATTATTTATGTATTTCCATGTCTTTATCTTCTAGTAGCATCATAAATATCTTTTCTAACAGGAAAAGGATGCATTTACTCGAGCAAACTTAAATGCAATTGCTGAAAAGCTGGTGCTTGATGAGCCAAACGAAGAACGTTGGACGCCATTATCATTTGTCTTTAAGCCTCATCACAACACAGTAACTGGAAATTATGACATAAATGTCCTAATTTCAGCTCTGGAAGAGAAGGGGAAGATTGTAGTGTGGCATGACCATCGGAATGGAGCTTCTTCAATCGATTTGGATGGACCTGAGGATACTCATAAACTTATGGGAATTGTACTTAACGTCTCAGTTAGAAGGTTTGGTGGCATTTGGAAAAGTAGGCACTGGGTTGCTCTGAGAAAGATCAACGGATTATGGTATAATTTGGACAGTGATCTGCCTGCTCCTCATCCGTTTAAGGACACTGATGAAGTAAGACAATTTTTGGATCATGTGATCGGTAACAATGGAGAAATTTTGCTTATTATGAATGACAGGAACTAA

mRNA sequence

ATGGCGAGCGAGGATTGCCGAGTTTATCACGAGAGACAAAAGCTACAGTTCTGTCTCTTGCACGCTCTCAATAACCTATTTCAGGAAAAGGATGCATTTACTCGAGCAAACTTAAATGCAATTGCTGAAAAGCTGGTGCTTGATGAGCCAAACGAAGAACGTTGGACGCCATTATCATTTGTCTTTAAGCCTCATCACAACACAGTAACTGGAAATTATGACATAAATGTCCTAATTTCAGCTCTGGAAGAGAAGGGGAAGATTGTAGTGTGGCATGACCATCGGAATGGAGCTTCTTCAATCGATTTGGATGGACCTGAGGATACTCATAAACTTATGGGAATTGTACTTAACGTCTCAGTTAGAAGGTTTGGTGGCATTTGGAAAAGTAGGCACTGGGTTGCTCTGAGAAAGATCAACGGATTATGGTATAATTTGGACAGTGATCTGCCTGCTCCTCATCCGTTTAAGGACACTGATGAAGTAAGACAATTTTTGGATCATGTGATCGGTAACAATGGAGAAATTTTGCTTATTATGAATGACAGGAACTAA

Coding sequence (CDS)

ATGGCGAGCGAGGATTGCCGAGTTTATCACGAGAGACAAAAGCTACAGTTCTGTCTCTTGCACGCTCTCAATAACCTATTTCAGGAAAAGGATGCATTTACTCGAGCAAACTTAAATGCAATTGCTGAAAAGCTGGTGCTTGATGAGCCAAACGAAGAACGTTGGACGCCATTATCATTTGTCTTTAAGCCTCATCACAACACAGTAACTGGAAATTATGACATAAATGTCCTAATTTCAGCTCTGGAAGAGAAGGGGAAGATTGTAGTGTGGCATGACCATCGGAATGGAGCTTCTTCAATCGATTTGGATGGACCTGAGGATACTCATAAACTTATGGGAATTGTACTTAACGTCTCAGTTAGAAGGTTTGGTGGCATTTGGAAAAGTAGGCACTGGGTTGCTCTGAGAAAGATCAACGGATTATGGTATAATTTGGACAGTGATCTGCCTGCTCCTCATCCGTTTAAGGACACTGATGAAGTAAGACAATTTTTGGATCATGTGATCGGTAACAATGGAGAAATTTTGCTTATTATGAATGACAGGAACTAA

Protein sequence

MASEDCRVYHERQKLQFCLLHALNNLFQEKDAFTRANLNAIAEKLVLDEPNEERWTPLSFVFKPHHNTVTGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVSVRRFGGIWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLDHVIGNNGEILLIMNDRN
BLAST of Bhi10G000619 vs. TAIR10
Match: AT2G29640.1 (JOSEPHIN-like protein)

HSP 1 Score: 261.5 bits (667), Expect = 3.8e-70
Identity = 118/167 (70.66%), Postives = 140/167 (83.83%), Query Frame = 0

Query: 1   MASEDCRVYHERQKLQFCLLHALNNLFQEKDAFTRANLNAIAEKLVLDEPNEERWTPLSF 60
           MA  + ++YHERQ+LQFCLLH LNNLFQ+KDAFT+ +LN+IAEKL  ++PN+E WTPLSF
Sbjct: 1   MADSESKIYHERQRLQFCLLHCLNNLFQDKDAFTKESLNSIAEKLETNDPNKETWTPLSF 60

Query: 61  VFKPHHNTVTGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVS 120
           V KPHHNT+TGNYD+NV+I+ALE KGK VVWHD R GASSIDLD   D   LMGIVLNV 
Sbjct: 61  VLKPHHNTITGNYDVNVMITALEGKGKSVVWHDKRIGASSIDLD---DADTLMGIVLNVP 120

Query: 121 VRRFGGIWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLD 168
           V+R+GG+W+SRHWV +RKING+WYNLDSDL  P  F+D DEVR FLD
Sbjct: 121 VKRYGGLWRSRHWVVVRKINGVWYNLDSDLVVPQLFRDDDEVRGFLD 164

BLAST of Bhi10G000619 vs. TAIR10
Match: AT3G54130.1 (Josephin family protein)

HSP 1 Score: 58.2 bits (139), Expect = 6.4e-09
Identity = 48/179 (26.82%), Postives = 77/179 (43.02%), Query Frame = 0

Query: 8   VYHERQKLQFCLLHALNNL-----FQEKD-AFTRANLNAIAEKLVLDEPNEERWTPLSFV 67
           +YHE Q+   C +H +N +     F E D A   A+L+    +++L+      + P  F+
Sbjct: 10  LYHEVQESNLCAVHCVNTVLQGPFFSEFDLAAVAADLDGKERQVMLEGAAVGGFAPGDFL 69

Query: 68  FKPHHN-TVTGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVS 127
            +  HN ++ G++ I VL  ALE      VW       +  D +  +   +L    +   
Sbjct: 70  AEESHNVSLGGDFSIQVLQKALE------VWDLQVIPLNCPDAEPAQIDPELESAFI--- 129

Query: 128 VRRFGGIWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLDHVIGNNGEILLI 180
                      HW  +RK+NG WYN DS L AP        +  FLD + G    I ++
Sbjct: 130 ------CHLHDHWFCIRKVNGEWYNFDSLLAAPQHLSKF-YLSAFLDSLKGAGWSIFIV 172

BLAST of Bhi10G000619 vs. Swiss-Prot
Match: sp|O82391|JOSL_ARATH (Josephin-like protein OS=Arabidopsis thaliana OX=3702 GN=At2g29640 PE=2 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 6.9e-69
Identity = 118/167 (70.66%), Postives = 140/167 (83.83%), Query Frame = 0

Query: 1   MASEDCRVYHERQKLQFCLLHALNNLFQEKDAFTRANLNAIAEKLVLDEPNEERWTPLSF 60
           MA  + ++YHERQ+LQFCLLH LNNLFQ+KDAFT+ +LN+IAEKL  ++PN+E WTPLSF
Sbjct: 1   MADSESKIYHERQRLQFCLLHCLNNLFQDKDAFTKESLNSIAEKLETNDPNKETWTPLSF 60

Query: 61  VFKPHHNTVTGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVS 120
           V KPHHNT+TGNYD+NV+I+ALE KGK VVWHD R GASSIDLD   D   LMGIVLNV 
Sbjct: 61  VLKPHHNTITGNYDVNVMITALEGKGKSVVWHDKRIGASSIDLD---DADTLMGIVLNVP 120

Query: 121 VRRFGGIWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLD 168
           V+R+GG+W+SRHWV +RKING+WYNLDSDL  P  F+D DEVR FLD
Sbjct: 121 VKRYGGLWRSRHWVVVRKINGVWYNLDSDLVVPQLFRDDDEVRGFLD 164

BLAST of Bhi10G000619 vs. Swiss-Prot
Match: sp|Q5EAE5|JOS1_BOVIN (Josephin-1 OS=Bos taurus OX=9913 GN=JOSD1 PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 7.2e-26
Identity = 66/181 (36.46%), Postives = 101/181 (55.80%), Query Frame = 0

Query: 7   RVYHERQKLQFCLLHALNNLFQEKDAFTRANLNAIAEKLVLDEPNEERWTPLSFVFKPHH 66
           ++YHE+Q+ + C LHALNN+FQ+ +AFTR  L  I ++L    PN         +  PH 
Sbjct: 25  QIYHEKQRRELCALHALNNVFQDSNAFTRETLQEIFQRL---SPNT--------MVTPHK 84

Query: 67  NTV--TGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVSVRRF 126
            ++   GNYD+NV+++AL+ KG   VW D R    +I L        +MG ++N+     
Sbjct: 85  KSMLGNGNYDVNVIMAALQTKGYEAVWWDKRRDVGAIALT------NVMGFIMNLPSSLC 144

Query: 127 GG----IWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLDHVI-GNNGEILLI 181
            G      K +HW+ +R++ G +YNLDS L  P       E+R+FL H + G N E+LL+
Sbjct: 145 WGPLKLPLKRQHWICVREVGGAYYNLDSKLKMPEWIGGKSELRKFLKHHLRGKNCELLLV 188

BLAST of Bhi10G000619 vs. Swiss-Prot
Match: sp|Q5BJY4|JOS1_RAT (Josephin-1 OS=Rattus norvegicus OX=10116 GN=Josd1 PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.2e-25
Identity = 67/181 (37.02%), Postives = 100/181 (55.25%), Query Frame = 0

Query: 7   RVYHERQKLQFCLLHALNNLFQEKDAFTRANLNAIAEKLVLDEPNEERWTPLSFVFKPHH 66
           ++YHE+Q+ + C LHALNN+FQ+ +AFTR  L  I ++L    PN         V  PH 
Sbjct: 25  QIYHEKQRRELCALHALNNVFQDSNAFTRETLQEIFQRL---SPNT--------VVTPHK 84

Query: 67  NTV--TGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVSVRRF 126
            ++   GNYD+NV+++AL+ KG   VW D R     I L        +MG ++N+     
Sbjct: 85  KSMLGNGNYDVNVIMAALQTKGYEAVWWDKRRDVGVIAL------ANVMGFIMNLPSSLC 144

Query: 127 GG----IWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLD-HVIGNNGEILLI 181
            G      K +HW+ +R++ G +YNLDS L  P       E+R+FL  H+ G N E+LL+
Sbjct: 145 WGPLKLPLKRQHWICVREVGGAYYNLDSKLKMPEWIGGESELRKFLKYHLRGKNCELLLV 188

BLAST of Bhi10G000619 vs. Swiss-Prot
Match: sp|Q15040|JOS1_HUMAN (Josephin-1 OS=Homo sapiens OX=9606 GN=JOSD1 PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.1e-25
Identity = 66/181 (36.46%), Postives = 100/181 (55.25%), Query Frame = 0

Query: 7   RVYHERQKLQFCLLHALNNLFQEKDAFTRANLNAIAEKLVLDEPNEERWTPLSFVFKPHH 66
           ++YHE+Q+ + C LHALNN+FQ+ +AFTR  L  I ++L    PN         +  PH 
Sbjct: 25  QIYHEKQRRELCALHALNNVFQDSNAFTRDTLQEIFQRL---SPNT--------MVTPHK 84

Query: 67  NTV--TGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVSVRRF 126
            ++   GNYD+NV+++AL+ KG   VW D R     I L        +MG ++N+     
Sbjct: 85  KSMLGNGNYDVNVIMAALQTKGYEAVWWDKRRDVGVIALT------NVMGFIMNLPSSLC 144

Query: 127 GG----IWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLDHVI-GNNGEILLI 181
            G      K +HW+ +R++ G +YNLDS L  P       E+R+FL H + G N E+LL+
Sbjct: 145 WGPLKLPLKRQHWICVREVGGAYYNLDSKLKMPEWIGGESELRKFLKHHLRGKNCELLLV 188

BLAST of Bhi10G000619 vs. Swiss-Prot
Match: sp|Q9DBJ6|JOS1_MOUSE (Josephin-1 OS=Mus musculus OX=10090 GN=Josd1 PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.1e-25
Identity = 66/181 (36.46%), Postives = 100/181 (55.25%), Query Frame = 0

Query: 7   RVYHERQKLQFCLLHALNNLFQEKDAFTRANLNAIAEKLVLDEPNEERWTPLSFVFKPHH 66
           ++YHE+Q+ + C LHALNN+FQ+ +AFTR  L  I ++L    PN         +  PH 
Sbjct: 25  QIYHEKQRRELCALHALNNVFQDSNAFTRETLQEIFQRL---SPNT--------MVTPHK 84

Query: 67  NTV--TGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVSVRRF 126
            ++   GNYD+NV+++AL+ KG   VW D R     I L        +MG ++N+     
Sbjct: 85  KSMLGNGNYDVNVIMAALQTKGYEAVWWDKRRDVGVIALT------NVMGFIMNLPSSLC 144

Query: 127 GG----IWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLD-HVIGNNGEILLI 181
            G      K +HW+ +R++ G +YNLDS L  P       E+R+FL  H+ G N E+LL+
Sbjct: 145 WGPLKLPLKRQHWICVREVGGAYYNLDSKLKMPEWIGGESELRKFLKYHLRGKNCELLLV 188

BLAST of Bhi10G000619 vs. TrEMBL
Match: tr|A0A1S4E4I3|A0A1S4E4I3_CUCME (josephin-like protein OS=Cucumis melo OX=3656 GN=LOC103502098 PE=4 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 4.9e-96
Identity = 166/184 (90.22%), Postives = 174/184 (94.57%), Query Frame = 0

Query: 1   MASEDCRVYHERQKLQFCLLHALNNLFQEKDAFTRANLNAIAEKLVLDEPNEERWTPLSF 60
           M  EDC VYHERQKLQ CLLHALNNLFQEKDAFTRANLNAIAEKLV DEPN+E WTPLS 
Sbjct: 2   MVREDCGVYHERQKLQLCLLHALNNLFQEKDAFTRANLNAIAEKLVRDEPNQETWTPLSI 61

Query: 61  VFKPHHNTVTGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVS 120
           VFKPHHNTVTGNYDINVLISALEEKGK VVWHDHRNGAS+IDLDGP+DT KLMGIVLNVS
Sbjct: 62  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASTIDLDGPDDTRKLMGIVLNVS 121

Query: 121 VRRFGGIWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLDHVIGNNGEILLIM 180
           VRRFGGIWKSRHWVALRKI+GLWYNLDSDLPAPHPFKDT+EVR+FLDHVI +NGEILL+M
Sbjct: 122 VRRFGGIWKSRHWVALRKIDGLWYNLDSDLPAPHPFKDTEEVREFLDHVINDNGEILLVM 181

Query: 181 NDRN 185
           NDRN
Sbjct: 182 NDRN 185

BLAST of Bhi10G000619 vs. TrEMBL
Match: tr|A0A0A0KBN9|A0A0A0KBN9_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G187930 PE=4 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 6.0e-94
Identity = 165/184 (89.67%), Postives = 171/184 (92.93%), Query Frame = 0

Query: 1   MASEDCRVYHERQKLQFCLLHALNNLFQEKDAFTRANLNAIAEKLVLDEPNEERWTPLSF 60
           MA EDC VYHERQKLQ CLLHALNNLFQEKDAFTRANLNAIAEKLV D PN+E WTPLS 
Sbjct: 1   MAREDCGVYHERQKLQLCLLHALNNLFQEKDAFTRANLNAIAEKLVRDVPNQETWTPLSI 60

Query: 61  VFKPHHNTVTGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVS 120
           VFKPHHNTVTGNYDINVLISALEEKGK VVWHDHRNGA SIDLDGP+ T KLMGIVLNVS
Sbjct: 61  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGAFSIDLDGPDHTCKLMGIVLNVS 120

Query: 121 VRRFGGIWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLDHVIGNNGEILLIM 180
           VRRFGGIWKSRHWVALRKI+GLWYNLDSDLP PHPFKDTDE+RQFLDHVI +NGEILL+M
Sbjct: 121 VRRFGGIWKSRHWVALRKIDGLWYNLDSDLPDPHPFKDTDELRQFLDHVINDNGEILLVM 180

Query: 181 NDRN 185
           NDRN
Sbjct: 181 NDRN 184

BLAST of Bhi10G000619 vs. TrEMBL
Match: tr|A0A2I4DE76|A0A2I4DE76_9ROSI (josephin-like protein OS=Juglans regia OX=51240 GN=LOC108979256 PE=4 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 7.1e-79
Identity = 137/183 (74.86%), Postives = 163/183 (89.07%), Query Frame = 0

Query: 1   MASEDCRVYHERQKLQFCLLHALNNLFQEKDAFTRANLNAIAEKLVLDEPNEERWTPLSF 60
           MASE+ ++YHERQ+LQFCLLH+LNNLFQ+KDAFTRA+LN IAEKLVLD+PN+  WTP+S 
Sbjct: 1   MASENTQIYHERQRLQFCLLHSLNNLFQQKDAFTRASLNEIAEKLVLDDPNKVTWTPISA 60

Query: 61  VFKPHHNTVTGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVS 120
           +FKPHHNT+TGNYDINVLI+ALE KGK V+WHD RNGAS I+LD  E+T  LMGIVLNVS
Sbjct: 61  LFKPHHNTITGNYDINVLIAALEGKGKSVIWHDRRNGASMINLDASEET--LMGIVLNVS 120

Query: 121 VRRFGGIWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLDHVIGNNGEILLIM 180
           VR+FGGIWK RHWVAL+KING+WYNLDSDL AP  FKDT+EVR FLD++I N GE+LL+M
Sbjct: 121 VRKFGGIWKGRHWVALKKINGVWYNLDSDLVAPETFKDTEEVRGFLDYIIDNGGEVLLVM 180

Query: 181 NDR 184
           N++
Sbjct: 181 NEK 181

BLAST of Bhi10G000619 vs. TrEMBL
Match: tr|M5WK40|M5WK40_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G060500 PE=4 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 1.9e-76
Identity = 132/177 (74.58%), Postives = 159/177 (89.83%), Query Frame = 0

Query: 7   RVYHERQKLQFCLLHALNNLFQEKDAFTRANLNAIAEKLVLDEPNEERWTPLSFVFKPHH 66
           +VYHERQ+LQFCLLH+LNNLFQ+KD FTR++LN IAEKLVL+EPN E WTPLS +FKPHH
Sbjct: 8   QVYHERQRLQFCLLHSLNNLFQQKDVFTRSSLNEIAEKLVLEEPNNETWTPLSVLFKPHH 67

Query: 67  NTVTGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVSVRRFGG 126
           N +TGNYDINVLI+ALE+KGK VVWHD RNGASSIDLDGPED   LMGI+LNV VR F G
Sbjct: 68  NALTGNYDINVLIAALEDKGKNVVWHDRRNGASSIDLDGPEDA--LMGILLNVPVRMFAG 127

Query: 127 IWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLDHVIGNNGEILLIMNDR 184
           +WKSRHWV+LRKI+G+WYNLDSDL AP  F+DT++VR+FLD++IG+ GE+LL+MN++
Sbjct: 128 LWKSRHWVSLRKIDGVWYNLDSDLVAPQAFEDTEKVREFLDYIIGHGGEVLLVMNNK 182

BLAST of Bhi10G000619 vs. TrEMBL
Match: tr|A0A1R3J6N8|A0A1R3J6N8_9ROSI (Machado-Joseph disease protein MJD OS=Corchorus olitorius OX=93759 GN=COLO4_19112 PE=4 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 2.1e-75
Identity = 130/183 (71.04%), Postives = 158/183 (86.34%), Query Frame = 0

Query: 1   MASEDCRVYHERQKLQFCLLHALNNLFQEKDAFTRANLNAIAEKLVLDEPNEERWTPLSF 60
           MASE  ++YHERQ+LQFCLLH LNNLFQ+KDAFTRANLN+IAEKLVLD+PN+E WTP S 
Sbjct: 1   MASEKNQIYHERQRLQFCLLHTLNNLFQQKDAFTRANLNSIAEKLVLDDPNKEIWTPFSL 60

Query: 61  VFKPHHNTVTGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVS 120
           VFKPHHN++TGNYDINVLI+ALEEKGK V+WHD RNGAS+IDL    D   LMGIV+NV 
Sbjct: 61  VFKPHHNSITGNYDINVLIAALEEKGKTVIWHDRRNGASAIDL----DDGMLMGIVINVP 120

Query: 121 VRRFGGIWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLDHVIGNNGEILLIM 180
           VRR+ G+WK RHW+ALRKI+G+WYNLDSDL APH FKD  EV ++LD+++ ++G++LL+ 
Sbjct: 121 VRRYAGLWKGRHWIALRKIDGVWYNLDSDLHAPHCFKDNGEVGEYLDYILNHDGQVLLVK 179

Query: 181 NDR 184
           NDR
Sbjct: 181 NDR 179

BLAST of Bhi10G000619 vs. NCBI nr
Match: XP_022148354.1 (josephin-like protein [Momordica charantia] >XP_022148355.1 josephin-like protein [Momordica charantia] >XP_022148357.1 josephin-like protein [Momordica charantia] >XP_022148358.1 josephin-like protein [Momordica charantia])

HSP 1 Score: 359.8 bits (922), Expect = 5.7e-96
Identity = 167/184 (90.76%), Postives = 176/184 (95.65%), Query Frame = 0

Query: 1   MASEDCRVYHERQKLQFCLLHALNNLFQEKDAFTRANLNAIAEKLVLDEPNEERWTPLSF 60
           MASEDCRVYHERQKLQFCLLHALNNLFQE DAFTRANLNAIAE LVL+EPN+E WTPLSF
Sbjct: 1   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRANLNAIAENLVLNEPNKEPWTPLSF 60

Query: 61  VFKPHHNTVTGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVS 120
           VFKPHHNTVTGNYDINVLISALEEKGK VVWHD RNGASSIDLDGPEDTHKLMGIVLNVS
Sbjct: 61  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDRRNGASSIDLDGPEDTHKLMGIVLNVS 120

Query: 121 VRRFGGIWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLDHVIGNNGEILLIM 180
           VRRFGGIWKSRHW+ALRKINGLWYNLDSDLP+P PFKDTDEVR+FLD+ IG++GEILL+M
Sbjct: 121 VRRFGGIWKSRHWIALRKINGLWYNLDSDLPSPRPFKDTDEVRKFLDYEIGHDGEILLVM 180

Query: 181 NDRN 185
           ND N
Sbjct: 181 NDGN 184

BLAST of Bhi10G000619 vs. NCBI nr
Match: XP_016903134.1 (PREDICTED: josephin-like protein [Cucumis melo] >XP_016903135.1 PREDICTED: josephin-like protein [Cucumis melo])

HSP 1 Score: 359.4 bits (921), Expect = 7.4e-96
Identity = 166/184 (90.22%), Postives = 174/184 (94.57%), Query Frame = 0

Query: 1   MASEDCRVYHERQKLQFCLLHALNNLFQEKDAFTRANLNAIAEKLVLDEPNEERWTPLSF 60
           M  EDC VYHERQKLQ CLLHALNNLFQEKDAFTRANLNAIAEKLV DEPN+E WTPLS 
Sbjct: 2   MVREDCGVYHERQKLQLCLLHALNNLFQEKDAFTRANLNAIAEKLVRDEPNQETWTPLSI 61

Query: 61  VFKPHHNTVTGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVS 120
           VFKPHHNTVTGNYDINVLISALEEKGK VVWHDHRNGAS+IDLDGP+DT KLMGIVLNVS
Sbjct: 62  VFKPHHNTVTGNYDINVLISALEEKGKSVVWHDHRNGASTIDLDGPDDTRKLMGIVLNVS 121

Query: 121 VRRFGGIWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLDHVIGNNGEILLIM 180
           VRRFGGIWKSRHWVALRKI+GLWYNLDSDLPAPHPFKDT+EVR+FLDHVI +NGEILL+M
Sbjct: 122 VRRFGGIWKSRHWVALRKIDGLWYNLDSDLPAPHPFKDTEEVREFLDHVINDNGEILLVM 181

Query: 181 NDRN 185
           NDRN
Sbjct: 182 NDRN 185

BLAST of Bhi10G000619 vs. NCBI nr
Match: XP_022986948.1 (josephin-like protein [Cucurbita maxima])

HSP 1 Score: 359.0 bits (920), Expect = 9.6e-96
Identity = 168/184 (91.30%), Postives = 175/184 (95.11%), Query Frame = 0

Query: 1   MASEDCRVYHERQKLQFCLLHALNNLFQEKDAFTRANLNAIAEKLVLDEPNEERWTPLSF 60
           MASEDCRVYHERQKLQFCLLHALNNLFQE +AFTRA+LNAIAEKLVLDEPN+E WTPLSF
Sbjct: 1   MASEDCRVYHERQKLQFCLLHALNNLFQENNAFTRAHLNAIAEKLVLDEPNKEPWTPLSF 60

Query: 61  VFKPHHNTVTGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVS 120
           VFKPHHN+VTGNYDINVLISALEEKGK VVWHDHRNGASSIDLDGPE THKLMGIVLNV 
Sbjct: 61  VFKPHHNSVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEATHKLMGIVLNVP 120

Query: 121 VRRFGGIWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLDHVIGNNGEILLIM 180
           VRRFGGIWKSRHWVALRKIN LWYNLDSDLP PH FKDTDEVRQFLD+VI +NGEILL+M
Sbjct: 121 VRRFGGIWKSRHWVALRKINELWYNLDSDLPTPHTFKDTDEVRQFLDNVINDNGEILLVM 180

Query: 181 NDRN 185
           NDRN
Sbjct: 181 NDRN 184

BLAST of Bhi10G000619 vs. NCBI nr
Match: XP_023512266.1 (uncharacterized protein LOC111777056 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 355.9 bits (912), Expect = 8.2e-95
Identity = 167/184 (90.76%), Postives = 173/184 (94.02%), Query Frame = 0

Query: 1   MASEDCRVYHERQKLQFCLLHALNNLFQEKDAFTRANLNAIAEKLVLDEPNEERWTPLSF 60
           MASEDCRVYHERQKLQFCLLHALNNLFQE DAFTRA+LNAI+EKLVLDEPN E WTPLSF
Sbjct: 2   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRAHLNAISEKLVLDEPNREPWTPLSF 61

Query: 61  VFKPHHNTVTGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVS 120
           VFKPHHN+VTGNYDINVLISALEEKGK VVWHDHRNGASSIDLDGPE THKLMGIVLNV 
Sbjct: 62  VFKPHHNSVTGNYDINVLISALEEKGKSVVWHDHRNGASSIDLDGPEATHKLMGIVLNVP 121

Query: 121 VRRFGGIWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLDHVIGNNGEILLIM 180
           VRRFGGIWKSRHWVALRKIN LWYNLDSDL  PH FKDTDEVRQFLD+VI +NGEILL+M
Sbjct: 122 VRRFGGIWKSRHWVALRKINELWYNLDSDLHTPHTFKDTDEVRQFLDNVINDNGEILLVM 181

Query: 181 NDRN 185
           NDRN
Sbjct: 182 NDRN 185

BLAST of Bhi10G000619 vs. NCBI nr
Match: XP_022944731.1 (josephin-like protein [Cucurbita moschata] >XP_022944732.1 josephin-like protein [Cucurbita moschata] >XP_022944733.1 josephin-like protein [Cucurbita moschata] >XP_022944734.1 josephin-like protein [Cucurbita moschata] >XP_022944735.1 josephin-like protein [Cucurbita moschata])

HSP 1 Score: 352.8 bits (904), Expect = 6.9e-94
Identity = 165/184 (89.67%), Postives = 172/184 (93.48%), Query Frame = 0

Query: 1   MASEDCRVYHERQKLQFCLLHALNNLFQEKDAFTRANLNAIAEKLVLDEPNEERWTPLSF 60
           MASEDCRVYHERQKLQFCLLHALNNLFQE DAFTRA+LNAI+EKLVLDEPN E WTPLSF
Sbjct: 1   MASEDCRVYHERQKLQFCLLHALNNLFQENDAFTRAHLNAISEKLVLDEPNREPWTPLSF 60

Query: 61  VFKPHHNTVTGNYDINVLISALEEKGKIVVWHDHRNGASSIDLDGPEDTHKLMGIVLNVS 120
           VFKPHHN+VTGNYDINVLISA+EEKGK VVWHDHRNGASSIDLDGPE THKLMGIVLNV 
Sbjct: 61  VFKPHHNSVTGNYDINVLISAMEEKGKSVVWHDHRNGASSIDLDGPEATHKLMGIVLNVP 120

Query: 121 VRRFGGIWKSRHWVALRKINGLWYNLDSDLPAPHPFKDTDEVRQFLDHVIGNNGEILLIM 180
           VRRFGGIWKSRHWVALRKIN  WYNLDSDL  PH FKDTDEVRQFLD+VI +NGEILL+M
Sbjct: 121 VRRFGGIWKSRHWVALRKINESWYNLDSDLHTPHTFKDTDEVRQFLDNVINDNGEILLVM 180

Query: 181 NDRN 185
           NDRN
Sbjct: 181 NDRN 184

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G29640.13.8e-7070.66JOSEPHIN-like protein[more]
AT3G54130.16.4e-0926.82Josephin family protein[more]
Match NameE-valueIdentityDescription
sp|O82391|JOSL_ARATH6.9e-6970.66Josephin-like protein OS=Arabidopsis thaliana OX=3702 GN=At2g29640 PE=2 SV=1[more]
sp|Q5EAE5|JOS1_BOVIN7.2e-2636.46Josephin-1 OS=Bos taurus OX=9913 GN=JOSD1 PE=2 SV=1[more]
sp|Q5BJY4|JOS1_RAT1.2e-2537.02Josephin-1 OS=Rattus norvegicus OX=10116 GN=Josd1 PE=2 SV=1[more]
sp|Q15040|JOS1_HUMAN2.1e-2536.46Josephin-1 OS=Homo sapiens OX=9606 GN=JOSD1 PE=1 SV=1[more]
sp|Q9DBJ6|JOS1_MOUSE2.1e-2536.46Josephin-1 OS=Mus musculus OX=10090 GN=Josd1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A1S4E4I3|A0A1S4E4I3_CUCME4.9e-9690.22josephin-like protein OS=Cucumis melo OX=3656 GN=LOC103502098 PE=4 SV=1[more]
tr|A0A0A0KBN9|A0A0A0KBN9_CUCSA6.0e-9489.67Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G187930 PE=4 SV=1[more]
tr|A0A2I4DE76|A0A2I4DE76_9ROSI7.1e-7974.86josephin-like protein OS=Juglans regia OX=51240 GN=LOC108979256 PE=4 SV=1[more]
tr|M5WK40|M5WK40_PRUPE1.9e-7674.58Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G060500 PE=4 SV=1[more]
tr|A0A1R3J6N8|A0A1R3J6N8_9ROSI2.1e-7571.04Machado-Joseph disease protein MJD OS=Corchorus olitorius OX=93759 GN=COLO4_1911... [more]
Match NameE-valueIdentityDescription
XP_022148354.15.7e-9690.76josephin-like protein [Momordica charantia] >XP_022148355.1 josephin-like protei... [more]
XP_016903134.17.4e-9690.22PREDICTED: josephin-like protein [Cucumis melo] >XP_016903135.1 PREDICTED: josep... [more]
XP_022986948.19.6e-9691.30josephin-like protein [Cucurbita maxima][more]
XP_023512266.18.2e-9590.76uncharacterized protein LOC111777056 [Cucurbita pepo subsp. pepo][more]
XP_022944731.16.9e-9489.67josephin-like protein [Cucurbita moschata] >XP_022944732.1 josephin-like protein... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004843thiol-dependent ubiquitin-specific protease activity
Vocabulary: Biological Process
TermDefinition
GO:0016579protein deubiquitination
Vocabulary: INTERPRO
TermDefinition
IPR006155Josephin
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016579 protein deubiquitination
biological_process GO:0006508 proteolysis
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004843 thiol-dependent ubiquitin-specific protease activity
molecular_function GO:0008242 omega peptidase activity
molecular_function GO:0016787 hydrolase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi10M000619Bhi10M000619mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006155Josephin domainSMARTSM01246Josephin_2coord: 12..182
e-value: 3.7E-65
score: 232.5
IPR006155Josephin domainPFAMPF02099Josephincoord: 13..179
e-value: 2.5E-41
score: 141.4
IPR006155Josephin domainPROSITEPS50957JOSEPHINcoord: 5..184
score: 47.068
NoneNo IPR availableGENE3DG3DSA:3.90.70.40coord: 71..179
e-value: 2.0E-17
score: 65.2
coord: 18..30
e-value: 2.0E-17
score: 65.2
NoneNo IPR availableGENE3DG3DSA:1.10.287.10coord: 32..70
e-value: 2.0E-17
score: 65.2
NoneNo IPR availablePANTHERPTHR13291:SF0JOSEPHIN-LIKE PROTEINcoord: 3..183
NoneNo IPR availablePANTHERPTHR13291JOSEPHIN 1, 2coord: 3..183

The following gene(s) are paralogous to this gene:

None