Bhi09G002411 (gene) Wax gourd

NameBhi09G002411
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionCoA ligase
Locationchr9 : 76290224 .. 76292281 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGGAACCGACCGATGCTCTGCCAATTGCGTTCCTCTCACTCCAATCACCTTCTTGGAGCGCGCCGCCGCCGTCTTTGGCGACAGAATTTCTCTCGTCTATGGAAATGTCCGCTTCACTTGGAGAGAGACTCTCGAACGATGTACCAAACTCGCTTCTGCTCTAGTTCAACTCGGAATCTCTCGCCGCGATGTGGTCTGTATTCTTAACTCTTCCATTTCGTTCGTTGATGTATTTTTAGTTTTTCGTTCTCTCTTTGTTCTATCAATGCATTTAAACCGAATTGGAGATAGGTTGAGAGTGTGCATTGTACATCACTGAAGATTATTGTTAGCTCGATCAATGGATATGGGTCTTATTTTGAAGCGGAACCACTAAATTTCTGCTGCTATTCTTTTTTGTTTCTTTTAATCTTGATTCTGAATCTTCCGTTTTTTATGATGTTGATTCTTCTTAGTATCTAAATTTATGTTACGAATCTAATTTGAAGTCTTGATGTTCTAATTATTTCGAGAACATTACGCTATTTGTTGATTATAATCCTTGACCTAGTTTTGCAACTAATTGCACATTTCACTCAAGTAGGTTGCTGTTTTGGCACCAAATGTTCCAGCTACATATGAGCTTCATTTTGCTGTACCAATGGCCAGTGCAGTTCTTTGTACACTCAACATGCGCCACGATGCAACAATGGTTTCTACATTGTTGAGTCATTCAGAAGCTAAAATCATTATAGTAGACTACCAATTCCTATCTATTGTAAAGGCAGCAATCAAAATTATGTCTAAGACGACGACAAAAGAGCTGCCTCGTATTGTCATTGTTAAAGAGTTTGATCATCCATCCTCCAACATCAACGGATTCGAGTCTGCTTCAAATGATTTAGAGTATGAAAGCCTTTTAGACACTGGAAAACTCGATTTCAAAATCAAACATCCTAAAGATGAATGGGATCCAATTTCTCTTAACTATACGTCAGGCACAACATCAAGACCAAAGGGTGTGATTTACTCTCATAGAGGTACTTATCTCAATACTCTATCCACAATCCTTCTGAATGATATGTCCTCAATGCCTGTGTATTTGTGGACTGTTCCAATGTTCCATTGCAACGGATGGTCTTTGACTTGGGGTGTAGCTGCACAAGGCGGCACAAACATCTGCCAAAGAAATGTGACTGCCAAGGAAATCTTTACCAATATTTCTCTACACAAGGTCACTCATATGGGCGGTGCACCGACTGTGTTGAACATGATCATCAATGCACCAATTAACGAACAGAAATCGCTTCCGTGTAAGGTAACCGTAATGACTGGTGGCGCTCCACCACCTTCTCATGTACTATATAAAATGAGAGAATTTGGATTCCTTATTGTCCATTGTTATGGTTTAACTGAAACATGTGGAACAGCAACAGTTTGCCATTGGAAACCTGAATGGGATGCCCTTCCTGATGATAAGCAAGCAAAGCTCAAATCTCGTCAAGGAATGCAACATATTGGGCTAGAAGGAGTGGAAATTAAAGATCCCATCACCATGGAAAGTGTTCCAGCTGATGGGAAAACCATGGGAGAAGTTATGTTCAGAGGTAACACTGTTATGAGTGGATATTTCAAAGATTTTAAAGCAACACAAGAAGCTTTTAATGGCGGATGGTTTAGAAGTGGGGACTTAGGAGTAAGGCATCCTGATGGTTATATAGAATTAAAGGATCGTTCAAAGGACATTATAATTTCTGGAGGTGAAAACATTAGCTCAATTGAAGTTGAATCTGTACTTTTCAATCATCCATCAGTTCTTGAAGCTGCTATTGTAGGAAGGCCTGATGATCATTGGGGCGAGACTCCATGTGCTTTTGTGAATCTCAAAGATGGTTTTAATGCTACAGAAGAAGAAATCATAAAGTTTTGCAGAGATAATCTTCCCCATTACATGGCTCCAAAAACTGTTGTGTTTAAAGATTTGCCTAAATCTTCTACTGGGAAAATTCAGAAGCTTATTCTTAAGGAGGAGGCCAAGGCCATGGATAGCCTTACTAAGAGGGTGACAAAGTAA

mRNA sequence

ATGGAAGGAACCGACCGATGCTCTGCCAATTGCGTTCCTCTCACTCCAATCACCTTCTTGGAGCGCGCCGCCGCCGTCTTTGGCGACAGAATTTCTCTCGTCTATGGAAATGTCCGCTTCACTTGGAGAGAGACTCTCGAACGATGTACCAAACTCGCTTCTGCTCTAGTTCAACTCGGAATCTCTCGCCGCGATGTGGTTGCTGTTTTGGCACCAAATGTTCCAGCTACATATGAGCTTCATTTTGCTGTACCAATGGCCAGTGCAGTTCTTTGTACACTCAACATGCGCCACGATGCAACAATGGTTTCTACATTGTTGAGTCATTCAGAAGCTAAAATCATTATAGTAGACTACCAATTCCTATCTATTGTAAAGGCAGCAATCAAAATTATGTCTAAGACGACGACAAAAGAGCTGCCTCGTATTGTCATTGTTAAAGAGTTTGATCATCCATCCTCCAACATCAACGGATTCGAGTCTGCTTCAAATGATTTAGAGTATGAAAGCCTTTTAGACACTGGAAAACTCGATTTCAAAATCAAACATCCTAAAGATGAATGGGATCCAATTTCTCTTAACTATACGTCAGGCACAACATCAAGACCAAAGGGTGTGATTTACTCTCATAGAGGTACTTATCTCAATACTCTATCCACAATCCTTCTGAATGATATGTCCTCAATGCCTGTGTATTTGTGGACTGTTCCAATGTTCCATTGCAACGGATGGTCTTTGACTTGGGGTGTAGCTGCACAAGGCGGCACAAACATCTGCCAAAGAAATGTGACTGCCAAGGAAATCTTTACCAATATTTCTCTACACAAGGTCACTCATATGGGCGGTGCACCGACTGTGTTGAACATGATCATCAATGCACCAATTAACGAACAGAAATCGCTTCCGTGTAAGGTAACCGTAATGACTGGTGGCGCTCCACCACCTTCTCATGTACTATATAAAATGAGAGAATTTGGATTCCTTATTGTCCATTGTTATGGTTTAACTGAAACATGTGGAACAGCAACAGTTTGCCATTGGAAACCTGAATGGGATGCCCTTCCTGATGATAAGCAAGCAAAGCTCAAATCTCGTCAAGGAATGCAACATATTGGGCTAGAAGGAGTGGAAATTAAAGATCCCATCACCATGGAAAGTGTTCCAGCTGATGGGAAAACCATGGGAGAAGTTATGTTCAGAGGTAACACTGTTATGAGTGGATATTTCAAAGATTTTAAAGCAACACAAGAAGCTTTTAATGGCGGATGGTTTAGAAGTGGGGACTTAGGAGTAAGGCATCCTGATGGTTATATAGAATTAAAGGATCGTTCAAAGGACATTATAATTTCTGGAGGTGAAAACATTAGCTCAATTGAAGTTGAATCTGTACTTTTCAATCATCCATCAGTTCTTGAAGCTGCTATTGTAGGAAGGCCTGATGATCATTGGGGCGAGACTCCATGTGCTTTTGTGAATCTCAAAGATGGTTTTAATGCTACAGAAGAAGAAATCATAAAGTTTTGCAGAGATAATCTTCCCCATTACATGGCTCCAAAAACTGTTGTGTTTAAAGATTTGCCTAAATCTTCTACTGGGAAAATTCAGAAGCTTATTCTTAAGGAGGAGGCCAAGGCCATGGATAGCCTTACTAAGAGGGTGACAAAGTAA

Coding sequence (CDS)

ATGGAAGGAACCGACCGATGCTCTGCCAATTGCGTTCCTCTCACTCCAATCACCTTCTTGGAGCGCGCCGCCGCCGTCTTTGGCGACAGAATTTCTCTCGTCTATGGAAATGTCCGCTTCACTTGGAGAGAGACTCTCGAACGATGTACCAAACTCGCTTCTGCTCTAGTTCAACTCGGAATCTCTCGCCGCGATGTGGTTGCTGTTTTGGCACCAAATGTTCCAGCTACATATGAGCTTCATTTTGCTGTACCAATGGCCAGTGCAGTTCTTTGTACACTCAACATGCGCCACGATGCAACAATGGTTTCTACATTGTTGAGTCATTCAGAAGCTAAAATCATTATAGTAGACTACCAATTCCTATCTATTGTAAAGGCAGCAATCAAAATTATGTCTAAGACGACGACAAAAGAGCTGCCTCGTATTGTCATTGTTAAAGAGTTTGATCATCCATCCTCCAACATCAACGGATTCGAGTCTGCTTCAAATGATTTAGAGTATGAAAGCCTTTTAGACACTGGAAAACTCGATTTCAAAATCAAACATCCTAAAGATGAATGGGATCCAATTTCTCTTAACTATACGTCAGGCACAACATCAAGACCAAAGGGTGTGATTTACTCTCATAGAGGTACTTATCTCAATACTCTATCCACAATCCTTCTGAATGATATGTCCTCAATGCCTGTGTATTTGTGGACTGTTCCAATGTTCCATTGCAACGGATGGTCTTTGACTTGGGGTGTAGCTGCACAAGGCGGCACAAACATCTGCCAAAGAAATGTGACTGCCAAGGAAATCTTTACCAATATTTCTCTACACAAGGTCACTCATATGGGCGGTGCACCGACTGTGTTGAACATGATCATCAATGCACCAATTAACGAACAGAAATCGCTTCCGTGTAAGGTAACCGTAATGACTGGTGGCGCTCCACCACCTTCTCATGTACTATATAAAATGAGAGAATTTGGATTCCTTATTGTCCATTGTTATGGTTTAACTGAAACATGTGGAACAGCAACAGTTTGCCATTGGAAACCTGAATGGGATGCCCTTCCTGATGATAAGCAAGCAAAGCTCAAATCTCGTCAAGGAATGCAACATATTGGGCTAGAAGGAGTGGAAATTAAAGATCCCATCACCATGGAAAGTGTTCCAGCTGATGGGAAAACCATGGGAGAAGTTATGTTCAGAGGTAACACTGTTATGAGTGGATATTTCAAAGATTTTAAAGCAACACAAGAAGCTTTTAATGGCGGATGGTTTAGAAGTGGGGACTTAGGAGTAAGGCATCCTGATGGTTATATAGAATTAAAGGATCGTTCAAAGGACATTATAATTTCTGGAGGTGAAAACATTAGCTCAATTGAAGTTGAATCTGTACTTTTCAATCATCCATCAGTTCTTGAAGCTGCTATTGTAGGAAGGCCTGATGATCATTGGGGCGAGACTCCATGTGCTTTTGTGAATCTCAAAGATGGTTTTAATGCTACAGAAGAAGAAATCATAAAGTTTTGCAGAGATAATCTTCCCCATTACATGGCTCCAAAAACTGTTGTGTTTAAAGATTTGCCTAAATCTTCTACTGGGAAAATTCAGAAGCTTATTCTTAAGGAGGAGGCCAAGGCCATGGATAGCCTTACTAAGAGGGTGACAAAGTAA

Protein sequence

MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLGISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQFLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDFKIKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFHCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQKSLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQAKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRPDDHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGKIQKLILKEEAKAMDSLTKRVTK
BLAST of Bhi09G002411 vs. Swiss-Prot
Match: sp|F4HUK6|AAE1_ARATH (Probable acyl-activating enzyme 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=2 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 2.9e-211
Identity = 348/552 (63.04%), Postives = 438/552 (79.35%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           MEGT +  AN VPLTPI+FL+R+            G+V++TWR+T +RC ++ASAL QLG
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSXXXXXXXXXXXXGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           IS  DVV+VLAPNVPA  ELHF VPMA A+LCTLN+RHD+++V+ LL HS  K+I  D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 FLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDFK 180
           FL I + A +I+S    K +P +V++ E    + +++  + +   +EYE ++  GK DF+
Sbjct: 123 FLQIAEGACEILSNKGDK-VPILVLIPE--PLTQSVSRKKRSEEMMEYEDVVAMGKSDFE 182

Query: 181 IKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFH 240
           +  P DE D IS+NYTSGTTS PKGV+YSHRG YLN+L+ +LLN+M S P YLWT PMFH
Sbjct: 183 VIRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFH 242

Query: 241 CNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQKS 300
           CNGW L WGV A GGTNIC RNVTAK IF NIS HKVTHMGGAPT+LNMIINAP +EQK 
Sbjct: 243 CNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKP 302

Query: 301 LPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQA 360
           LP KV+ +TG APPP+HV++KM E GF + H YGLTET G  T+C WKPEWD+LP ++QA
Sbjct: 303 LPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQA 362

Query: 361 KLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAFN 420
           K+K+RQG+ H+GLE +++KDP+TM ++PADG TMGEV+FRGNTVM+GY K+ +AT+EAF 
Sbjct: 363 KMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFK 422

Query: 421 GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRPD 480
           GGWF SGDLGV+HPDGYIELKDRSKDIIISGGENISSIEVES LF HP VLEAA+V RPD
Sbjct: 423 GGWFWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPD 482

Query: 481 DHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGKIQKLILK 540
           ++WGET CAFV LKDG  A+ EE+I +CRD LPHYMAP+++VF+DLPK+STGK+QK +L+
Sbjct: 483 EYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLR 542

Query: 541 EEAKAMDSLTKR 553
            +AKA+ SL+K+
Sbjct: 543 TKAKALVSLSKK 551

BLAST of Bhi09G002411 vs. Swiss-Prot
Match: sp|Q9SEY5|AAE2_ARATH (Probable acyl-activating enzyme 2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=2 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 1.0e-200
Identity = 341/556 (61.33%), Postives = 420/556 (75.54%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           +EG  R  AN  PL+PITFLER+A V+ DR SLV+G+V+ TW +T +RC +LASAL  LG
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLG 107

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           ISR DVVA LAPNVPA +ELHFAVPMA  +LC LN R D + +S LL+HSEAKI+ VD+Q
Sbjct: 108 ISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQ 167

Query: 121 FLSIVKAAIKIMSKT--TTKEL----PRIVIVKEFDHPSSNINGFESASNDLEYESLLDT 180
            L I   A+ +++K+  T K L                                 +LL +
Sbjct: 168 LLEIAHGALDLLAKSDKTRKSLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTLLKS 227

Query: 181 GKLDFKIKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLW 240
           G  +F+I  P+ EWDPIS+NYTSGTTSRPKGV+YSHRG YLN+L+T+ L+ MS  PVYLW
Sbjct: 228 GDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVYLW 287

Query: 241 TVPMFHCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAP 300
           TVPMFHCNGW L WGVAAQGGTNIC R V+ K IF NI++HKVTHMGGAPTVLNMI+N  
Sbjct: 288 TVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYT 347

Query: 301 INEQKSLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDAL 360
           + E K LP +V +MTGG+PP   +L KM E GF + H YGLTET G  T C WKPEWD+L
Sbjct: 348 VTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSL 407

Query: 361 PDDKQAKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKA 420
             +++ KLK+RQG+QH+GLEG+++KDP+TME+VP DG TMGEVMFRGNTVMSGYFKD +A
Sbjct: 408 SLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEA 467

Query: 421 TQEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAA 480
           T++AF G WF SGDL V++PDGYIE+KDR KD+IISGGENISS+EVE VL +H +VLEAA
Sbjct: 468 TRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLEAA 527

Query: 481 IVGRPDDHWGETPCAFVNLKDGFNATE-EEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGK 540
           +V RPD HWG+TPC FV LK+GF+  + EEII FCRD+LPHYMAPKT+VF D+PK+STGK
Sbjct: 528 VVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTSTGK 587

Query: 541 IQKLILKEEAKAMDSL 550
           +QK +L+++A  M SL
Sbjct: 588 VQKYLLRKKADEMGSL 603

BLAST of Bhi09G002411 vs. Swiss-Prot
Match: sp|Q9C8D4|AAE11_ARATH (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 7.6e-164
Identity = 278/554 (50.18%), Postives = 390/554 (70.40%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           M+    C AN VPLTPITFL+RA+  + +R S++YG  RFTW +T +RC +LA++L+ L 
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           I+R DVV++LAPNVPA YE+HF+VPM  AVL  +N R DA  ++ +L H+E KI+ VDY+
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 FLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGK---- 180
           F  +++  ++++    ++  PRI+++ E D  +      +  S +L+YE L+  G+    
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTT------KPFSKELDYEGLIRKGEPTPS 180

Query: 181 LDFKIKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTV 240
               +    +E DPISLNYTSGTT+ PKGV+ SH+G YL+ LS+I+  +M   PVYLWT+
Sbjct: 181 SSASMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTL 240

Query: 241 PMFHCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPIN 300
           PMFHCNGW+ TW VAA+GGTN+C R+VTA EI+ NI LH VTHM   PTV   ++     
Sbjct: 241 PMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRT 300

Query: 301 EQKSLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPD 360
           +Q      V V+TGG+ PP+ ++ K+ + GF ++H YGLTE  G    C W+ EW+ LP+
Sbjct: 301 DQSPKSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPE 360

Query: 361 DKQAKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQ 420
            +Q +L+ RQG++++ L  V++K+  T+ESVP DGKTMGE++ +G+++M GY K+ KAT 
Sbjct: 361 HQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATS 420

Query: 421 EAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIV 480
           EAF  GW  +GD+GV HPDGY+E+KDRSKDIIISGGENISSIEVE VL+ +  VLEAA+V
Sbjct: 421 EAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVV 480

Query: 481 GRPDDHWGETPCAFVNLK---DGFNATEEEIIKFCRDNLPHYMAPKTVV-FKDLPKSSTG 540
             P   WGETPCAFV LK   +G   +E ++IK+CR+N+PH+M PK VV F++LPK+S G
Sbjct: 481 AMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNG 540

Query: 541 KIQKLILKEEAKAM 547
           KI K  L++ AKA+
Sbjct: 541 KILKSKLRDIAKAL 548

BLAST of Bhi09G002411 vs. Swiss-Prot
Match: sp|Q9SFW5|AEE21_ARATH (Probable acyl-activating enzyme 21 OS=Arabidopsis thaliana OX=3702 GN=AEE21 PE=3 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 7.2e-162
Identity = 285/558 (51.08%), Postives = 383/558 (68.64%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           MEGT RCSAN VPL+PI+FLERAA VFG R S+VYG++++TW +T +RC +LASAL  LG
Sbjct: 1   MEGTMRCSANYVPLSPISFLERAAVVFGSRTSVVYGDIQYTWHQTRDRCVRLASALSDLG 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           +SR DVVA LAPNVPA  EL+F  PMA AVLC LN   D+ M++  L  ++ K+  VD +
Sbjct: 61  LSRHDVVAALAPNVPALCELYFGAPMAGAVLCVLNTTFDSQMLAMALEKTKPKVFFVDSE 120

Query: 121 FLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDFK 180
           FLS+ + ++ ++S    +E P I+ + E        N  E +  + +YE  L TG  +FK
Sbjct: 121 FLSVAEESLSLLS--NIEEKPLIITITE--------NPTEQSKYE-QYEDFLSTGNPNFK 180

Query: 181 IKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFH 240
              P DE DPI+LN+TSGTTS PK V+YSHRG YLN  +  ++N+M  MPVYL TVPM+H
Sbjct: 181 PIRPVDECDPIALNFTSGTTSTPKCVVYSHRGAYLNATAVGVMNEMKPMPVYLCTVPMYH 240

Query: 241 CNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQKS 300
           C+GW   W V A GG  +C R V  + IF +I  HKVT+ GG+P VLNMI NA  + +KS
Sbjct: 241 CSGWCYIWTVTAFGGVIVCLREVNDEVIFDSIVKHKVTNFGGSPPVLNMIANARDSVKKS 300

Query: 301 LPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQA 360
            P  V VM+GG+ PP  V+ K+++ GF ++  YG +E  G  T C W PEW+ LP+++  
Sbjct: 301 FPWTVQVMSGGSSPP-EVMLKLKKLGFKVMMAYGCSEVYGLGTACLWMPEWETLPEEESL 360

Query: 361 KLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAFN 420
           +LK+R G+ H   E V++ DP TM+SVP DGKT+  +  RGNTVMSGYFKD +AT+ AF 
Sbjct: 361 RLKARDGLNHFAKEAVDVLDPTTMKSVPHDGKTIRVIALRGNTVMSGYFKDKEATEAAFR 420

Query: 421 GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRPD 480
           GGW+ S D+GV  PDGYI+ KDRS+D+I  GGE + S E+E +L++HP+V +A +VGRPD
Sbjct: 421 GGWYWSRDMGVIDPDGYIQFKDRSQDVITCGGEIVGSKEIEGILYSHPAVYDAGVVGRPD 480

Query: 481 DHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPH---YMAPKTVVFKDLPKSSTGKIQKL 540
           +  GE+ CAFV LK+G  A EEEII+FC+  L +    M PKTVVF D+PK+ TGKI+K 
Sbjct: 481 ETLGESMCAFVKLKEGAEAREEEIIEFCKRKLGNKNMKMIPKTVVFSDVPKTPTGKIRKN 540

Query: 541 ILKEEAKAMDSLTKRVTK 556
           +L++ AK M  +  R  +
Sbjct: 541 VLRKMAKDMGYVQLRAVE 546

BLAST of Bhi09G002411 vs. Swiss-Prot
Match: sp|Q8VZF1|AEE7_ARATH (Acetate/butyrate--CoA ligase AAE7, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE7 PE=1 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 1.8e-160
Identity = 276/545 (50.64%), Postives = 376/545 (68.99%), Query Frame = 0

Query: 9   ANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLGISRRDVVA 68
           AN   LTP+ FL+RAA V   R S+++G+  +TWR+T +RC +LASAL    I     VA
Sbjct: 17  ANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASALADRSIGPGSTVA 76

Query: 69  VLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQFLSIVKAA 128
           ++APN+PA YE HF VPM  AVL  +N+R +A  V+ LLSHS++ +I+VD +F ++ + +
Sbjct: 77  IIAPNIPAMYEAHFGVPMCGAVLNCVNIRLNAPTVAFLLSHSQSSVIMVDQEFFTLAEDS 136

Query: 129 IKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASND-LEYESLLDTGKLDFKIKHPKDE 188
           +++M +       R +++   DH  +  +   + S   +EYE  L TG  ++  + P DE
Sbjct: 137 LRLMEEKAGSSFKRPLLIVIGDHTCAPESLNRALSKGAIEYEDFLATGDPNYPWQPPADE 196

Query: 189 WDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFHCNGWSLT 248
           W  I+L YTSGTT+ PKGV+  HRG Y+  LS  L+  M    VYLWT+PMFHCNGW   
Sbjct: 197 WQSIALGYTSGTTASPKGVVLHHRGAYIMALSNPLIWGMQDGAVYLWTLPMFHCNGWCFP 256

Query: 249 WGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQ-KSLPCKVT 308
           W +A   GT+IC R VTAKE+++ I+ +KVTH   AP VLN I+NAP  +    LP  V 
Sbjct: 257 WSLAVLSGTSICLRQVTAKEVYSMIAKYKVTHFCAAPVVLNAIVNAPKEDTILPLPHTVH 316

Query: 309 VMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQAKLKSRQ 368
           VMT GA PP  VL+ M + GF + H YGL+ET G +TVC WKPEWD+LP + QAKL +RQ
Sbjct: 317 VMTAGAAPPPSVLFSMNQKGFRVAHTYGLSETYGPSTVCAWKPEWDSLPPETQAKLNARQ 376

Query: 369 GMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAFNGGWFRS 428
           G+++ G+E +++ D  T + VPADGKT GE++FRGN VM GY K+ +A +E F GGWF S
Sbjct: 377 GVRYTGMEQLDVIDTQTGKPVPADGKTAGEIVFRGNMVMKGYLKNPEANKETFAGGWFHS 436

Query: 429 GDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRPDDHWGET 488
           GD+ V+HPD YIE+KDRSKD+IISGGENISS+EVE+V+++HP+VLEA++V RPD+ W E+
Sbjct: 437 GDIAVKHPDNYIEIKDRSKDVIISGGENISSVEVENVVYHHPAVLEASVVARPDERWQES 496

Query: 489 PCAFVNLKDGF-----NATEEEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGKIQKLILKE 547
           PCAFV LK  +     N   ++I+KFCR+ LP Y  PK+VVF  LPK++TGKIQK IL+ 
Sbjct: 497 PCAFVTLKSDYEKHDQNKLAQDIMKFCREKLPAYWVPKSVVFGPLPKTATGKIQKHILRT 556

BLAST of Bhi09G002411 vs. TAIR10
Match: AT1G20560.1 (acyl activating enzyme 1)

HSP 1 Score: 736.1 bits (1899), Expect = 1.6e-212
Identity = 348/552 (63.04%), Postives = 438/552 (79.35%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           MEGT +  AN VPLTPI+FL+R+            G+V++TWR+T +RC ++ASAL QLG
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSXXXXXXXXXXXXGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           IS  DVV+VLAPNVPA  ELHF VPMA A+LCTLN+RHD+++V+ LL HS  K+I  D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 FLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDFK 180
           FL I + A +I+S    K +P +V++ E    + +++  + +   +EYE ++  GK DF+
Sbjct: 123 FLQIAEGACEILSNKGDK-VPILVLIPE--PLTQSVSRKKRSEEMMEYEDVVAMGKSDFE 182

Query: 181 IKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFH 240
           +  P DE D IS+NYTSGTTS PKGV+YSHRG YLN+L+ +LLN+M S P YLWT PMFH
Sbjct: 183 VIRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFH 242

Query: 241 CNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQKS 300
           CNGW L WGV A GGTNIC RNVTAK IF NIS HKVTHMGGAPT+LNMIINAP +EQK 
Sbjct: 243 CNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKP 302

Query: 301 LPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQA 360
           LP KV+ +TG APPP+HV++KM E GF + H YGLTET G  T+C WKPEWD+LP ++QA
Sbjct: 303 LPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQA 362

Query: 361 KLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAFN 420
           K+K+RQG+ H+GLE +++KDP+TM ++PADG TMGEV+FRGNTVM+GY K+ +AT+EAF 
Sbjct: 363 KMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFK 422

Query: 421 GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRPD 480
           GGWF SGDLGV+HPDGYIELKDRSKDIIISGGENISSIEVES LF HP VLEAA+V RPD
Sbjct: 423 GGWFWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPD 482

Query: 481 DHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGKIQKLILK 540
           ++WGET CAFV LKDG  A+ EE+I +CRD LPHYMAP+++VF+DLPK+STGK+QK +L+
Sbjct: 483 EYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLR 542

Query: 541 EEAKAMDSLTKR 553
            +AKA+ SL+K+
Sbjct: 543 TKAKALVSLSKK 551

BLAST of Bhi09G002411 vs. TAIR10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 701.0 bits (1808), Expect = 5.7e-202
Identity = 341/556 (61.33%), Postives = 420/556 (75.54%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           +EG  R  AN  PL+PITFLER+A V+ DR SLV+G+V+ TW +T +RC +LASAL  LG
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLG 107

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           ISR DVVA LAPNVPA +ELHFAVPMA  +LC LN R D + +S LL+HSEAKI+ VD+Q
Sbjct: 108 ISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQ 167

Query: 121 FLSIVKAAIKIMSKT--TTKEL----PRIVIVKEFDHPSSNINGFESASNDLEYESLLDT 180
            L I   A+ +++K+  T K L                                 +LL +
Sbjct: 168 LLEIAHGALDLLAKSDKTRKSLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTLLKS 227

Query: 181 GKLDFKIKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLW 240
           G  +F+I  P+ EWDPIS+NYTSGTTSRPKGV+YSHRG YLN+L+T+ L+ MS  PVYLW
Sbjct: 228 GDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVYLW 287

Query: 241 TVPMFHCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAP 300
           TVPMFHCNGW L WGVAAQGGTNIC R V+ K IF NI++HKVTHMGGAPTVLNMI+N  
Sbjct: 288 TVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYT 347

Query: 301 INEQKSLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDAL 360
           + E K LP +V +MTGG+PP   +L KM E GF + H YGLTET G  T C WKPEWD+L
Sbjct: 348 VTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSL 407

Query: 361 PDDKQAKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKA 420
             +++ KLK+RQG+QH+GLEG+++KDP+TME+VP DG TMGEVMFRGNTVMSGYFKD +A
Sbjct: 408 SLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEA 467

Query: 421 TQEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAA 480
           T++AF G WF SGDL V++PDGYIE+KDR KD+IISGGENISS+EVE VL +H +VLEAA
Sbjct: 468 TRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLEAA 527

Query: 481 IVGRPDDHWGETPCAFVNLKDGFNATE-EEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGK 540
           +V RPD HWG+TPC FV LK+GF+  + EEII FCRD+LPHYMAPKT+VF D+PK+STGK
Sbjct: 528 VVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTSTGK 587

Query: 541 IQKLILKEEAKAMDSL 550
           +QK +L+++A  M SL
Sbjct: 588 VQKYLLRKKADEMGSL 603

BLAST of Bhi09G002411 vs. TAIR10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 578.6 bits (1490), Expect = 4.2e-165
Identity = 278/554 (50.18%), Postives = 390/554 (70.40%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           M+    C AN VPLTPITFL+RA+  + +R S++YG  RFTW +T +RC +LA++L+ L 
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           I+R DVV++LAPNVPA YE+HF+VPM  AVL  +N R DA  ++ +L H+E KI+ VDY+
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 FLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGK---- 180
           F  +++  ++++    ++  PRI+++ E D  +      +  S +L+YE L+  G+    
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTT------KPFSKELDYEGLIRKGEPTPS 180

Query: 181 LDFKIKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTV 240
               +    +E DPISLNYTSGTT+ PKGV+ SH+G YL+ LS+I+  +M   PVYLWT+
Sbjct: 181 SSASMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTL 240

Query: 241 PMFHCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPIN 300
           PMFHCNGW+ TW VAA+GGTN+C R+VTA EI+ NI LH VTHM   PTV   ++     
Sbjct: 241 PMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRT 300

Query: 301 EQKSLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPD 360
           +Q      V V+TGG+ PP+ ++ K+ + GF ++H YGLTE  G    C W+ EW+ LP+
Sbjct: 301 DQSPKSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPE 360

Query: 361 DKQAKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQ 420
            +Q +L+ RQG++++ L  V++K+  T+ESVP DGKTMGE++ +G+++M GY K+ KAT 
Sbjct: 361 HQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATS 420

Query: 421 EAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIV 480
           EAF  GW  +GD+GV HPDGY+E+KDRSKDIIISGGENISSIEVE VL+ +  VLEAA+V
Sbjct: 421 EAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVV 480

Query: 481 GRPDDHWGETPCAFVNLK---DGFNATEEEIIKFCRDNLPHYMAPKTVV-FKDLPKSSTG 540
             P   WGETPCAFV LK   +G   +E ++IK+CR+N+PH+M PK VV F++LPK+S G
Sbjct: 481 AMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNG 540

Query: 541 KIQKLILKEEAKAM 547
           KI K  L++ AKA+
Sbjct: 541 KILKSKLRDIAKAL 548

BLAST of Bhi09G002411 vs. TAIR10
Match: AT1G76290.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 572.0 bits (1473), Expect = 4.0e-163
Identity = 285/558 (51.08%), Postives = 383/558 (68.64%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           MEGT RCSAN VPL+PI+FLERAA VFG R S+VYG++++TW +T +RC +LASAL  LG
Sbjct: 1   MEGTMRCSANYVPLSPISFLERAAVVFGSRTSVVYGDIQYTWHQTRDRCVRLASALSDLG 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           +SR DVVA LAPNVPA  EL+F  PMA AVLC LN   D+ M++  L  ++ K+  VD +
Sbjct: 61  LSRHDVVAALAPNVPALCELYFGAPMAGAVLCVLNTTFDSQMLAMALEKTKPKVFFVDSE 120

Query: 121 FLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDFK 180
           FLS+ + ++ ++S    +E P I+ + E        N  E +  + +YE  L TG  +FK
Sbjct: 121 FLSVAEESLSLLS--NIEEKPLIITITE--------NPTEQSKYE-QYEDFLSTGNPNFK 180

Query: 181 IKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFH 240
              P DE DPI+LN+TSGTTS PK V+YSHRG YLN  +  ++N+M  MPVYL TVPM+H
Sbjct: 181 PIRPVDECDPIALNFTSGTTSTPKCVVYSHRGAYLNATAVGVMNEMKPMPVYLCTVPMYH 240

Query: 241 CNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQKS 300
           C+GW   W V A GG  +C R V  + IF +I  HKVT+ GG+P VLNMI NA  + +KS
Sbjct: 241 CSGWCYIWTVTAFGGVIVCLREVNDEVIFDSIVKHKVTNFGGSPPVLNMIANARDSVKKS 300

Query: 301 LPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQA 360
            P  V VM+GG+ PP  V+ K+++ GF ++  YG +E  G  T C W PEW+ LP+++  
Sbjct: 301 FPWTVQVMSGGSSPP-EVMLKLKKLGFKVMMAYGCSEVYGLGTACLWMPEWETLPEEESL 360

Query: 361 KLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAFN 420
           +LK+R G+ H   E V++ DP TM+SVP DGKT+  +  RGNTVMSGYFKD +AT+ AF 
Sbjct: 361 RLKARDGLNHFAKEAVDVLDPTTMKSVPHDGKTIRVIALRGNTVMSGYFKDKEATEAAFR 420

Query: 421 GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRPD 480
           GGW+ S D+GV  PDGYI+ KDRS+D+I  GGE + S E+E +L++HP+V +A +VGRPD
Sbjct: 421 GGWYWSRDMGVIDPDGYIQFKDRSQDVITCGGEIVGSKEIEGILYSHPAVYDAGVVGRPD 480

Query: 481 DHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPH---YMAPKTVVFKDLPKSSTGKIQKL 540
           +  GE+ CAFV LK+G  A EEEII+FC+  L +    M PKTVVF D+PK+ TGKI+K 
Sbjct: 481 ETLGESMCAFVKLKEGAEAREEEIIEFCKRKLGNKNMKMIPKTVVFSDVPKTPTGKIRKN 540

Query: 541 ILKEEAKAMDSLTKRVTK 556
           +L++ AK M  +  R  +
Sbjct: 541 VLRKMAKDMGYVQLRAVE 546

BLAST of Bhi09G002411 vs. TAIR10
Match: AT3G16910.1 (acyl-activating enzyme 7)

HSP 1 Score: 567.4 bits (1461), Expect = 9.8e-162
Identity = 276/545 (50.64%), Postives = 376/545 (68.99%), Query Frame = 0

Query: 9   ANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLGISRRDVVA 68
           AN   LTP+ FL+RAA V   R S+++G+  +TWR+T +RC +LASAL    I     VA
Sbjct: 17  ANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASALADRSIGPGSTVA 76

Query: 69  VLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQFLSIVKAA 128
           ++APN+PA YE HF VPM  AVL  +N+R +A  V+ LLSHS++ +I+VD +F ++ + +
Sbjct: 77  IIAPNIPAMYEAHFGVPMCGAVLNCVNIRLNAPTVAFLLSHSQSSVIMVDQEFFTLAEDS 136

Query: 129 IKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASND-LEYESLLDTGKLDFKIKHPKDE 188
           +++M +       R +++   DH  +  +   + S   +EYE  L TG  ++  + P DE
Sbjct: 137 LRLMEEKAGSSFKRPLLIVIGDHTCAPESLNRALSKGAIEYEDFLATGDPNYPWQPPADE 196

Query: 189 WDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFHCNGWSLT 248
           W  I+L YTSGTT+ PKGV+  HRG Y+  LS  L+  M    VYLWT+PMFHCNGW   
Sbjct: 197 WQSIALGYTSGTTASPKGVVLHHRGAYIMALSNPLIWGMQDGAVYLWTLPMFHCNGWCFP 256

Query: 249 WGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQ-KSLPCKVT 308
           W +A   GT+IC R VTAKE+++ I+ +KVTH   AP VLN I+NAP  +    LP  V 
Sbjct: 257 WSLAVLSGTSICLRQVTAKEVYSMIAKYKVTHFCAAPVVLNAIVNAPKEDTILPLPHTVH 316

Query: 309 VMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQAKLKSRQ 368
           VMT GA PP  VL+ M + GF + H YGL+ET G +TVC WKPEWD+LP + QAKL +RQ
Sbjct: 317 VMTAGAAPPPSVLFSMNQKGFRVAHTYGLSETYGPSTVCAWKPEWDSLPPETQAKLNARQ 376

Query: 369 GMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAFNGGWFRS 428
           G+++ G+E +++ D  T + VPADGKT GE++FRGN VM GY K+ +A +E F GGWF S
Sbjct: 377 GVRYTGMEQLDVIDTQTGKPVPADGKTAGEIVFRGNMVMKGYLKNPEANKETFAGGWFHS 436

Query: 429 GDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRPDDHWGET 488
           GD+ V+HPD YIE+KDRSKD+IISGGENISS+EVE+V+++HP+VLEA++V RPD+ W E+
Sbjct: 437 GDIAVKHPDNYIEIKDRSKDVIISGGENISSVEVENVVYHHPAVLEASVVARPDERWQES 496

Query: 489 PCAFVNLKDGF-----NATEEEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGKIQKLILKE 547
           PCAFV LK  +     N   ++I+KFCR+ LP Y  PK+VVF  LPK++TGKIQK IL+ 
Sbjct: 497 PCAFVTLKSDYEKHDQNKLAQDIMKFCREKLPAYWVPKSVVFGPLPKTATGKIQKHILRT 556

BLAST of Bhi09G002411 vs. TrEMBL
Match: tr|A0A1S3BV31|A0A1S3BV31_CUCME (probable acyl-activating enzyme 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103493806 PE=4 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 2.8e-288
Identity = 488/552 (88.41%), Postives = 517/552 (93.66%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           MEG   CSAN +PLTPITFLERAAAVF DRISLVYGNVRFTW ETL+RCTK ASALV LG
Sbjct: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           ISR DVVAVLAPNVPATYELHFAVPMA A+LCTLNMRHDA M+STLL HSEAKII+VD+Q
Sbjct: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120

Query: 121 FLSIVKAAIKIMSKTT-TKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDF 180
           +L IVKAAI+IMSK T  +ELPRIVIV+EFDHPSSNINGF+SAS+DLEYESLL+TG LDF
Sbjct: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180

Query: 181 KIKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           +I+ P DEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF
Sbjct: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240

Query: 241 HCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQK 300
           HCNGWS+TWGVAAQGGTNICQRNV AKEIF NISLHKVTHMGGAPTVLNMI+NAPI EQK
Sbjct: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300

Query: 301 SLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQ 360
            LP KVTVMTGGAPPPS++LYKMRE GFLIVHCYGLTETCG AT C WKPEWD+LPDDKQ
Sbjct: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360

Query: 361 AKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAF 420
           AKLKSRQGMQHIG+EG++IK+PITMESVPADGKTMGEVM RGNTVMSGY KD KAT+EAF
Sbjct: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENIS+IEVESVLFNHPSVLEAAIVGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFK-DLPKSSTGKIQKLI 540
           DDHWGETPCAFVNLKDG NAT+EEII+FCRD LPHYMAPKTVVFK DLPKSSTGKIQKLI
Sbjct: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540

Query: 541 LKEEAKAMDSLT 551
           LKEEAKAM SL+
Sbjct: 541 LKEEAKAMGSLS 552

BLAST of Bhi09G002411 vs. TrEMBL
Match: tr|A0A0A0L3B4|A0A0A0L3B4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G658540 PE=4 SV=1)

HSP 1 Score: 984.9 bits (2545), Expect = 7.2e-284
Identity = 486/552 (88.04%), Postives = 514/552 (93.12%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           MEG  RCSAN VPLTPITFLERAAAVFGDRISLVYGNVRFTWRETL+RCTK ASALV +G
Sbjct: 1   MEGI-RCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVG 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           ISR DVVAVLAPNVPATYELHFAVPMA A+LCTLNMRHDA MVSTLL HSEAKII+ D+Q
Sbjct: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQ 120

Query: 121 FLSIVKAAIKIMSKT-TTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDF 180
           +L IVKAAI+IMSKT   +ELPRIVIV+EFDHPSSNINGF+SAS+DLEYESLL+TG LDF
Sbjct: 121 YLHIVKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180

Query: 181 KIKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           +I+ P DE DPISLNYTSGTTSRPKGVIYSHRGTYLNTLST+LLNDMSSM VYLWTVPMF
Sbjct: 181 EIRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMF 240

Query: 241 HCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQK 300
           HCNGWS+TWGVAAQGGTNICQRNV AKEIF NISLH VTHMGGAPTVLNMI+NAPI EQK
Sbjct: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQK 300

Query: 301 SLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQ 360
            L  KVTVMTGGAPPPS++LYKMRE GFLIVHCYGLTET G AT CHWKPEWD+LPD+KQ
Sbjct: 301 PLLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQ 360

Query: 361 AKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAF 420
           AKLKSRQGMQHIG+EGV+IK+PITMESVPADGKTMGEVM RGNTVM GY KD KAT+EAF
Sbjct: 361 AKLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENIS+IEVESVLFNHPSVLEAAIVGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFK-DLPKSSTGKIQKLI 540
           DDHWGETPCAFVNLK G NATEEEIIKFCRDNLPHYMAPKTVVFK DLPKSSTGKIQKLI
Sbjct: 481 DDHWGETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540

Query: 541 LKEEAKAMDSLT 551
           LKEEAKAM +L+
Sbjct: 541 LKEEAKAMGTLS 551

BLAST of Bhi09G002411 vs. TrEMBL
Match: tr|A0A1S3BUH2|A0A1S3BUH2_CUCME (probable acyl-activating enzyme 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103493805 PE=4 SV=1)

HSP 1 Score: 898.7 bits (2321), Expect = 6.7e-258
Identity = 435/555 (78.38%), Postives = 490/555 (88.29%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           M+G  RCSAN VPLTPITFLER+AAV+GDRISLVYG V++TWR+TLERCT+LASALV+ G
Sbjct: 1   MDGIHRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRDTLERCTRLASALVRTG 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           I+R DVVA L PN+PA YELHFAVPMA AVLC+LN RHDA MVSTLLSHSEAKII+VDYQ
Sbjct: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 121 FLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDFK 180
              IV  AI  MS+   K LPR+VI++E+D P S+I+   SA   LE+ES L +G+LDF+
Sbjct: 121 LEHIVTGAINTMSERKEK-LPRVVIIQEYDQPPSHIDRPGSA---LEFESFLASGELDFE 180

Query: 181 IKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFH 240
           I+ P+DEWDPI+LNYTSGTTSRPKGVIYSHRG YLN LS +LLNDM S+PVYLWTVPMFH
Sbjct: 181 IRRPRDEWDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWTVPMFH 240

Query: 241 CNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQKS 300
           CNGW LTWGVAAQ GTNICQRNVTAKEIF NISLHKVTHMGGAPTV NMIINAPI+EQK 
Sbjct: 241 CNGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKP 300

Query: 301 LPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQA 360
           LP +VT+M+GGAPPPSHVLYK++  GF IVH YGLTET G  TVC WKPEWD+LP DKQA
Sbjct: 301 LPRQVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPGTVCSWKPEWDSLPQDKQA 360

Query: 361 KLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAFN 420
           KL SRQG+QHIGLE V+IKD +TM+SVPADGKTMGEVM RGNTVMSGY KD KATQEAFN
Sbjct: 361 KLNSRQGLQHIGLEAVDIKDSVTMKSVPADGKTMGEVMLRGNTVMSGYLKDLKATQEAFN 420

Query: 421 GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRPD 480
           GGWFRSGDLGV+HPDGYIELKDRSKDIIISGGENIS+IEVESVLF+HPSVL+AA+VGRPD
Sbjct: 421 GGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPD 480

Query: 481 DHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGKIQKLILK 540
           DHWGETPCAF+ LKDG +ATEEEIIKFCR++LPHYMAP++VVF++LPK+STGK QK ILK
Sbjct: 481 DHWGETPCAFLKLKDGCSATEEEIIKFCREHLPHYMAPRSVVFRELPKTSTGKTQKFILK 540

Query: 541 EEAKAMDSLTKRVTK 556
           +EAKAM SL KRV+K
Sbjct: 541 KEAKAMGSLPKRVSK 551

BLAST of Bhi09G002411 vs. TrEMBL
Match: tr|A0A0A0L1B6|A0A0A0L1B6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G658530 PE=4 SV=1)

HSP 1 Score: 897.9 bits (2319), Expect = 1.2e-257
Identity = 433/555 (78.02%), Postives = 491/555 (88.47%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           M+G+ RCSAN VPLTPI+FLER+AAV+GDRISLVYG V++TWR+TL+RCT+LASALV+ G
Sbjct: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           I+R DVVA L PN+PA YELHFAVPMA AVLC+LN RHDA MVSTLLSHSEAKII+VDYQ
Sbjct: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 121 FLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDFK 180
              IV  AIK MS+   K LPR+VI++E+D P S I+   SA   LE+ES L +GKL+F+
Sbjct: 121 LEHIVTGAIKAMSERKEK-LPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFE 180

Query: 181 IKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFH 240
           I+ P+DE DPI+LNYTSGTTSRPKGVI+SHRG YLN+LS +LLNDM S+PVYLWTVPMFH
Sbjct: 181 IRRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFH 240

Query: 241 CNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQKS 300
           CNGW LTWGVAAQ GTNICQRNVTAKEIF NISLHKVTHMGGAPTV NMIINAPI+EQK 
Sbjct: 241 CNGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKP 300

Query: 301 LPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQA 360
           LP +VT+M+GGAPPPSHVLYK++  GF IVH YGLTET G ATVC WKPEWD+LP DKQA
Sbjct: 301 LPREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQA 360

Query: 361 KLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAFN 420
           KL SRQG+QHIGLE  ++KDP+TMESVPADGKTMGEVM RGNTVMSGY KD KAT+EAFN
Sbjct: 361 KLNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLKDLKATREAFN 420

Query: 421 GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRPD 480
           GGWFRSGDLGV+HPDGYIELKDRSKDIIISGGENIS+IEVESVLF+HPSVL+AA+VGRPD
Sbjct: 421 GGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPD 480

Query: 481 DHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGKIQKLILK 540
           DHWGETPCAFV LKDG +ATE EIIKFCR++LPHYMAP++VVF+DLPK+STGK QK ILK
Sbjct: 481 DHWGETPCAFVKLKDGCSATEGEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKTQKFILK 540

Query: 541 EEAKAMDSLTKRVTK 556
           +EAKAM SL KRV+K
Sbjct: 541 KEAKAMGSLPKRVSK 554

BLAST of Bhi09G002411 vs. TrEMBL
Match: tr|A0A2I4FXM7|A0A2I4FXM7_9ROSI (probable acyl-activating enzyme 1, peroxisomal isoform X1 OS=Juglans regia OX=51240 GN=LOC109002926 PE=4 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 1.1e-244
Identity = 402/552 (72.83%), Postives = 472/552 (85.51%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           MEG  RCSAN VPLTPI+FLER+A V+ DR+S+VYG+VR+TWRETLERCT+LASA+ QLG
Sbjct: 8   MEGAIRCSANYVPLTPISFLERSAMVYRDRLSVVYGDVRYTWRETLERCTRLASAIAQLG 67

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           ISR DVVA +APN+PA YELHF VPMA AVLCTLN+RHD+ MVSTLL HSEAK+I VDYQ
Sbjct: 68  ISRGDVVAAMAPNIPAMYELHFGVPMAGAVLCTLNVRHDSAMVSTLLRHSEAKLIFVDYQ 127

Query: 121 FLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDFK 180
           FL I + AI I+SKT T +LP +V++ E D PS+N +   S S +LEYE  L TG+ DF+
Sbjct: 128 FLHIAQGAIDILSKTRT-QLPLLVLIPESDPPSANTSNAISRSRNLEYEGFLATGRPDFE 187

Query: 181 IKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFH 240
           ++ P DEWDPISLNYTSGTTS PKGVI+SHRG YLN+L+  LLN+M SMPVYLW VPMFH
Sbjct: 188 VRRPNDEWDPISLNYTSGTTSSPKGVIFSHRGAYLNSLAAALLNEMGSMPVYLWCVPMFH 247

Query: 241 CNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQKS 300
           CNGW LTW VAAQGGTN+CQRNVTA+ IF NIS HKVTH GGAPTVLNM+INA  +E + 
Sbjct: 248 CNGWCLTWAVAAQGGTNVCQRNVTARGIFENISRHKVTHFGGAPTVLNMVINATASEIRP 307

Query: 301 LPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQA 360
           LP KV VMTGGAPPPSHVL+KM E GF + H YGLTET G  TVC WKPEW++LP + QA
Sbjct: 308 LPGKVAVMTGGAPPPSHVLFKMEELGFNVTHAYGLTETYGPGTVCTWKPEWNSLPREAQA 367

Query: 361 KLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAFN 420
           K+K+RQG+ H+GLE ++IKDP+TM+SVP D KTMGEVMFRGNTVM+GY KD KATQ+AFN
Sbjct: 368 KIKARQGLHHLGLEEIDIKDPVTMKSVPPDAKTMGEVMFRGNTVMNGYLKDSKATQDAFN 427

Query: 421 GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRPD 480
           GGWFRSGDL V+HPDGYIELKDRSKDIIISGGENIS+IEVESV+F HP+VLEAA+VGRPD
Sbjct: 428 GGWFRSGDLAVKHPDGYIELKDRSKDIIISGGENISTIEVESVIFGHPAVLEAAVVGRPD 487

Query: 481 DHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGKIQKLILK 540
           D+WGETPCAFV LKDG++ + EEIIKFCR++LPHYMAP+TV+F+DLPK+STGK QK +L+
Sbjct: 488 DYWGETPCAFVKLKDGYSVSAEEIIKFCREHLPHYMAPRTVIFEDLPKTSTGKAQKFVLR 547

Query: 541 EEAKAMDSLTKR 553
           E+AKAM SLT +
Sbjct: 548 EKAKAMGSLTNK 558

BLAST of Bhi09G002411 vs. NCBI nr
Match: XP_008452948.1 (PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis melo])

HSP 1 Score: 999.6 bits (2583), Expect = 4.2e-288
Identity = 488/552 (88.41%), Postives = 517/552 (93.66%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           MEG   CSAN +PLTPITFLERAAAVF DRISLVYGNVRFTW ETL+RCTK ASALV LG
Sbjct: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           ISR DVVAVLAPNVPATYELHFAVPMA A+LCTLNMRHDA M+STLL HSEAKII+VD+Q
Sbjct: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120

Query: 121 FLSIVKAAIKIMSKTT-TKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDF 180
           +L IVKAAI+IMSK T  +ELPRIVIV+EFDHPSSNINGF+SAS+DLEYESLL+TG LDF
Sbjct: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180

Query: 181 KIKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           +I+ P DEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF
Sbjct: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240

Query: 241 HCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQK 300
           HCNGWS+TWGVAAQGGTNICQRNV AKEIF NISLHKVTHMGGAPTVLNMI+NAPI EQK
Sbjct: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300

Query: 301 SLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQ 360
            LP KVTVMTGGAPPPS++LYKMRE GFLIVHCYGLTETCG AT C WKPEWD+LPDDKQ
Sbjct: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360

Query: 361 AKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAF 420
           AKLKSRQGMQHIG+EG++IK+PITMESVPADGKTMGEVM RGNTVMSGY KD KAT+EAF
Sbjct: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENIS+IEVESVLFNHPSVLEAAIVGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFK-DLPKSSTGKIQKLI 540
           DDHWGETPCAFVNLKDG NAT+EEII+FCRD LPHYMAPKTVVFK DLPKSSTGKIQKLI
Sbjct: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540

Query: 541 LKEEAKAMDSLT 551
           LKEEAKAM SL+
Sbjct: 541 LKEEAKAMGSLS 552

BLAST of Bhi09G002411 vs. NCBI nr
Match: XP_004145598.1 (PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis sativus] >KGN55494.1 hypothetical protein Csa_4G658540 [Cucumis sativus])

HSP 1 Score: 984.9 bits (2545), Expect = 1.1e-283
Identity = 486/552 (88.04%), Postives = 514/552 (93.12%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           MEG  RCSAN VPLTPITFLERAAAVFGDRISLVYGNVRFTWRETL+RCTK ASALV +G
Sbjct: 1   MEGI-RCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVG 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           ISR DVVAVLAPNVPATYELHFAVPMA A+LCTLNMRHDA MVSTLL HSEAKII+ D+Q
Sbjct: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQ 120

Query: 121 FLSIVKAAIKIMSKT-TTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDF 180
           +L IVKAAI+IMSKT   +ELPRIVIV+EFDHPSSNINGF+SAS+DLEYESLL+TG LDF
Sbjct: 121 YLHIVKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180

Query: 181 KIKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           +I+ P DE DPISLNYTSGTTSRPKGVIYSHRGTYLNTLST+LLNDMSSM VYLWTVPMF
Sbjct: 181 EIRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMF 240

Query: 241 HCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQK 300
           HCNGWS+TWGVAAQGGTNICQRNV AKEIF NISLH VTHMGGAPTVLNMI+NAPI EQK
Sbjct: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQK 300

Query: 301 SLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQ 360
            L  KVTVMTGGAPPPS++LYKMRE GFLIVHCYGLTET G AT CHWKPEWD+LPD+KQ
Sbjct: 301 PLLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQ 360

Query: 361 AKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAF 420
           AKLKSRQGMQHIG+EGV+IK+PITMESVPADGKTMGEVM RGNTVM GY KD KAT+EAF
Sbjct: 361 AKLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENIS+IEVESVLFNHPSVLEAAIVGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFK-DLPKSSTGKIQKLI 540
           DDHWGETPCAFVNLK G NATEEEIIKFCRDNLPHYMAPKTVVFK DLPKSSTGKIQKLI
Sbjct: 481 DDHWGETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540

Query: 541 LKEEAKAMDSLT 551
           LKEEAKAM +L+
Sbjct: 541 LKEEAKAMGTLS 551

BLAST of Bhi09G002411 vs. NCBI nr
Match: XP_022137950.1 (probable acyl-activating enzyme 1, peroxisomal [Momordica charantia])

HSP 1 Score: 947.2 bits (2447), Expect = 2.5e-272
Identity = 454/551 (82.40%), Postives = 499/551 (90.56%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           MEG +RCSANCV LTPITFLER+AAV+GDR SLVYG VR+TWR+TL+RCT+LASALV+ G
Sbjct: 1   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           ISR DVVA LAPN+PA YELHFAVPMA AVLCTLNMRHDA MVSTLLSHSEAKII VDYQ
Sbjct: 61  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 120

Query: 121 FLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDFK 180
           FL +VK AIK+MSKTT K LP +V+++E+D PSS ING +SA  DLE+ESLL TG++DFK
Sbjct: 121 FLPMVKRAIKLMSKTTEK-LPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDFK 180

Query: 181 IKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFH 240
           I+ PKDEWDPISLNYTSGTTSRPKGVIYSHRG YLNTLST+LLNDM S+PVYLWTVPMFH
Sbjct: 181 IRRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPMFH 240

Query: 241 CNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQKS 300
           CNGW  +WGVAAQGGTNICQR VTAKEIF NIS H VTHMGGAPTVLNMIINAPI+EQK 
Sbjct: 241 CNGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQKP 300

Query: 301 LPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQA 360
           LP KVTVMTGGAPPPSHVLYKMR  GFLI+H YGLTET G ATVC WKPEWD+LP+D+QA
Sbjct: 301 LPGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQQA 360

Query: 361 KLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAFN 420
           KL SRQG+QH+GLEGV+IKDP+TMES PADGKTMGEVMFRGNTVMSGY KD KATQEAFN
Sbjct: 361 KLNSRQGLQHVGLEGVDIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEAFN 420

Query: 421 GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRPD 480
           GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVL++HPS+LE AIVGRPD
Sbjct: 421 GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGRPD 480

Query: 481 DHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGKIQKLILK 540
           DHWGETPCAFV LKDG +A  EEIIKFCR++LPHYMAP++VVF+DLPK+STGKIQK +LK
Sbjct: 481 DHWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKIQKFVLK 540

Query: 541 EEAKAMDSLTK 552
           EEA AM SL +
Sbjct: 541 EEATAMGSLQR 550

BLAST of Bhi09G002411 vs. NCBI nr
Match: XP_023522426.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 941.8 bits (2433), Expect = 1.1e-270
Identity = 460/556 (82.73%), Postives = 505/556 (90.83%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           M+G  RCSAN  PLTPITFLER+AAV+G+R+SLV+G VR+TW ETLERCT+LASALV +G
Sbjct: 48  MDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLERCTRLASALVDIG 107

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           ISRRDVVA LAPN+PA YELHFAVPMA AVLCTLNMRHDA M+ TLL HSEAKII VDYQ
Sbjct: 108 ISRRDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMICTLLRHSEAKIIFVDYQ 167

Query: 121 FLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDFK 180
           FL IVK AI+IMSK TT+ELPR+VI+KE D  SS  NGF+S+S+DLE+E LL TGKLDF+
Sbjct: 168 FLHIVKGAIEIMSK-TTEELPRVVIIKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDFE 227

Query: 181 IKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFH 240
           I+ P+DE DPISLNYTSGTTSRPKGVIYSHRG YLNTLSTIL+N+M SMPVYLWTVPMFH
Sbjct: 228 IRRPQDEDDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTILINNMCSMPVYLWTVPMFH 287

Query: 241 CNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQKS 300
           CNGW LTWGVAAQGGTNICQRNVTA++IF NIS HKVTHMGGAPTVLNMIINAPI EQK 
Sbjct: 288 CNGWCLTWGVAAQGGTNICQRNVTARDIFANISEHKVTHMGGAPTVLNMIINAPITEQKL 347

Query: 301 LPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQA 360
           LP  VTVMTGGAPP SHVL+KM+EFGFLIVH YGLTET G ATVC WKPEWD+LP+DKQA
Sbjct: 348 LPGTVTVMTGGAPPASHVLHKMKEFGFLIVHSYGLTETHGPATVCSWKPEWDSLPEDKQA 407

Query: 361 KLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAFN 420
           KLKSRQG+QH+GL+GV+IKDPITMES PADGKT+GEVM RGNTVMSGYFKD KAT+EAF+
Sbjct: 408 KLKSRQGLQHVGLQGVDIKDPITMESAPADGKTIGEVMLRGNTVMSGYFKDLKATEEAFS 467

Query: 421 GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRPD 480
           GGWFR+GDLGVRHPDGYIELKDRSKDIIISGGENISSIEVES LF+HPSVLEAAIVGRPD
Sbjct: 468 GGWFRTGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESALFSHPSVLEAAIVGRPD 527

Query: 481 DHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGKIQKLILK 540
           DHWGETPCAFV LKDG +A+EEEIIKFCRDNLPHYMAP++VVF+DLPK+STGKIQK  LK
Sbjct: 528 DHWGETPCAFVKLKDGRSASEEEIIKFCRDNLPHYMAPRSVVFRDLPKTSTGKIQKFKLK 587

Query: 541 EEAKAMDSL-TKRVTK 556
           EEAKAM SL TKR TK
Sbjct: 588 EEAKAMGSLTTKRSTK 602

BLAST of Bhi09G002411 vs. NCBI nr
Match: XP_022982524.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita maxima])

HSP 1 Score: 937.2 bits (2421), Expect = 2.6e-269
Identity = 457/556 (82.19%), Postives = 504/556 (90.65%), Query Frame = 0

Query: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60
           M+G  RCSAN  PLTPITFLER+AAV+G+R+SLV+G VR+TW ETLERCT+LASALV +G
Sbjct: 1   MDGMHRCSANYSPLTPITFLERSAAVYGERLSLVHGTVRYTWTETLERCTRLASALVDVG 60

Query: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120
           ISRRDVVA LAPN+PA YELHFAVPMA AVLCTLNMRHDA M+ TLL HSEAKII VDYQ
Sbjct: 61  ISRRDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMICTLLRHSEAKIIFVDYQ 120

Query: 121 FLSIVKAAIKIMSKTTTKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDFK 180
           FL IVK AI+IMSK TT+ELPR+VI+KE D  SS  NGF+S+S+DLE+E LL TGKLDF+
Sbjct: 121 FLHIVKGAIEIMSK-TTEELPRVVIIKESDQQSSYTNGFDSSSDDLEFERLLGTGKLDFE 180

Query: 181 IKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFH 240
           I+ P+DE DPISLNYTSGTTSRPKGVIYSHRG YLNTLSTIL+N+M S PVYLWTVPMFH
Sbjct: 181 IRRPQDEDDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTILINNMCSTPVYLWTVPMFH 240

Query: 241 CNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQKS 300
           CNGW LTWGVAAQGGTNICQRNVTA++IF N+S HKVTHMGGAPTVLNMIINAPI EQK 
Sbjct: 241 CNGWCLTWGVAAQGGTNICQRNVTARDIFANMSEHKVTHMGGAPTVLNMIINAPITEQKL 300

Query: 301 LPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQA 360
           LP  VTVMTGGAPP SHVL+KM+EFGFLIVH YGLTET G ATVC WKPEWD+LP+D+QA
Sbjct: 301 LPGTVTVMTGGAPPASHVLHKMKEFGFLIVHSYGLTETHGPATVCSWKPEWDSLPEDEQA 360

Query: 361 KLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAFN 420
           KLKSRQG+QH+GL+GV+IKDPITMES PADGKT+GEVM RGNTVMSGYFKD KAT+EAF+
Sbjct: 361 KLKSRQGLQHVGLQGVDIKDPITMESAPADGKTIGEVMLRGNTVMSGYFKDLKATEEAFS 420

Query: 421 GGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRPD 480
           GGWFR+GDLGVRHPDGYIELKDRSKDIIISGGENISSIEVES LF+HPSVLEAAIVGRPD
Sbjct: 421 GGWFRTGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESALFSHPSVLEAAIVGRPD 480

Query: 481 DHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFKDLPKSSTGKIQKLILK 540
           DHWGETPCAFV LKDG +A+EEEIIKFCRDNLPHYMAP++VVF+DLPK+STGKIQK  LK
Sbjct: 481 DHWGETPCAFVKLKDGRSASEEEIIKFCRDNLPHYMAPRSVVFRDLPKTSTGKIQKFTLK 540

Query: 541 EEAKAMDSL-TKRVTK 556
           EEAKAM SL TKR TK
Sbjct: 541 EEAKAMGSLTTKRSTK 555

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|F4HUK6|AAE1_ARATH2.9e-21163.04Probable acyl-activating enzyme 1, peroxisomal OS=Arabidopsis thaliana OX=3702 G... [more]
sp|Q9SEY5|AAE2_ARATH1.0e-20061.33Probable acyl-activating enzyme 2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=2 S... [more]
sp|Q9C8D4|AAE11_ARATH7.6e-16450.18Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11... [more]
sp|Q9SFW5|AEE21_ARATH7.2e-16251.08Probable acyl-activating enzyme 21 OS=Arabidopsis thaliana OX=3702 GN=AEE21 PE=3... [more]
sp|Q8VZF1|AEE7_ARATH1.8e-16050.64Acetate/butyrate--CoA ligase AAE7, peroxisomal OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
AT1G20560.11.6e-21263.04acyl activating enzyme 1[more]
AT2G17650.15.7e-20261.33AMP-dependent synthetase and ligase family protein[more]
AT1G66120.14.2e-16550.18AMP-dependent synthetase and ligase family protein[more]
AT1G76290.14.0e-16351.08AMP-dependent synthetase and ligase family protein[more]
AT3G16910.19.8e-16250.64acyl-activating enzyme 7[more]
Match NameE-valueIdentityDescription
tr|A0A1S3BV31|A0A1S3BV31_CUCME2.8e-28888.41probable acyl-activating enzyme 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103... [more]
tr|A0A0A0L3B4|A0A0A0L3B4_CUCSA7.2e-28488.04Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G658540 PE=4 SV=1[more]
tr|A0A1S3BUH2|A0A1S3BUH2_CUCME6.7e-25878.38probable acyl-activating enzyme 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103... [more]
tr|A0A0A0L1B6|A0A0A0L1B6_CUCSA1.2e-25778.02Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G658530 PE=4 SV=1[more]
tr|A0A2I4FXM7|A0A2I4FXM7_9ROSI1.1e-24472.83probable acyl-activating enzyme 1, peroxisomal isoform X1 OS=Juglans regia OX=51... [more]
Match NameE-valueIdentityDescription
XP_008452948.14.2e-28888.41PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis melo][more]
XP_004145598.11.1e-28388.04PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis sativus] >KGN... [more]
XP_022137950.12.5e-27282.40probable acyl-activating enzyme 1, peroxisomal [Momordica charantia][more]
XP_023522426.11.1e-27082.73probable acyl-activating enzyme 1, peroxisomal [Cucurbita pepo subsp. pepo][more]
XP_022982524.12.6e-26982.19probable acyl-activating enzyme 1, peroxisomal [Cucurbita maxima][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: INTERPRO
TermDefinition
IPR020845AMP-binding_CS
IPR025110AMP-bd_C
IPR000873AMP-dep_Synth/Lig
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi09M002411Bhi09M002411mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.30.300.310coord: 446..548
e-value: 1.0E-31
score: 111.2
NoneNo IPR availableGENE3DG3DSA:3.40.50.12780coord: 5..445
e-value: 2.6E-97
score: 328.0
NoneNo IPR availablePANTHERPTHR43859FAMILY NOT NAMEDcoord: 1..550
NoneNo IPR availablePANTHERPTHR43859:SF5ACYL-ACTIVATING ENZYME 2-RELATEDcoord: 1..550
NoneNo IPR availableCDDcd12118ttLC_FACS_AEE21_likecoord: 12..541
e-value: 0.0
score: 860.415
NoneNo IPR availableSUPERFAMILYSSF56801Acetyl-CoA synthetase-likecoord: 10..546
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 20..450
e-value: 5.0E-92
score: 308.6
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 459..533
e-value: 1.5E-21
score: 77.0
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 193..204

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None