Bhi09G000071 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi09G000071
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	Gibberellin 3-beta-dioxygenase
Location	chr9 : 1649324 .. 1649839 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGCCAACCCGAAAATGAGTGATGTCTTCAAAACCCACCCCGTCCATGTCCCCACCATCAAAAACCTCGACTCTTTCCAACAACTCCCCGACACTTATGATTGGATTCAACCCGAATCTTTCCCTTCCTTTAGAGACTCCAATATTCTTCTCTCCGATTCCATCCCCTTAATTGACCTTTCTCTCCCCAATGCCCCTGAGCTCATTACCAATGCATTACGAACTTGGGGGGTATTCCAGGTCATTAACCATGGCGTCCCCATGTCCCTCATCCACTCCATGGAATCCACTGTCAATACCCTCTTTGATCTTCCCTCTTCCCAGAAGCTTAAGGCTGCTCGTTCCCCCGACGGCGTCGCTGGCTACGGCCTTGTCCGTATTTCCCCCTTCTTCACTAAGCGTATGTGGTCTGAAGGCTTCACCATCAACGGTTCCCCTCTTCAACACTTCCAAAAACTCTGGCCTCATGACTACACCAAATACTGGTATTACTATTACTATCACGAACTGTGA

mRNA sequence

ATGGCCGCCAACCCGAAAATGAGTGATGTCTTCAAAACCCACCCCGTCCATGTCCCCACCATCAAAAACCTCGACTCTTTCCAACAACTCCCCGACACTTATGATTGGATTCAACCCGAATCTTTCCCTTCCTTTAGAGACTCCAATATTCTTCTCTCCGATTCCATCCCCTTAATTGACCTTTCTCTCCCCAATGCCCCTGAGCTCATTACCAATGCATTACGAACTTGGGGGGTATTCCAGGTCATTAACCATGGCGTCCCCATGTCCCTCATCCACTCCATGGAATCCACTGTCAATACCCTCTTTGATCTTCCCTCTTCCCAGAAGCTTAAGGCTGCTCGTTCCCCCGACGGCGTCGCTGGCTACGGCCTTGTCCGTATTTCCCCCTTCTTCACTAAGCGTATGTGGTCTGAAGGCTTCACCATCAACGGTTCCCCTCTTCAACACTTCCAAAAACTCTGGCCTCATGACTACACCAAATACTGGTATTACTATTACTATCACGAACTGTGA

Coding sequence (CDS)

ATGGCCGCCAACCCGAAAATGAGTGATGTCTTCAAAACCCACCCCGTCCATGTCCCCACCATCAAAAACCTCGACTCTTTCCAACAACTCCCCGACACTTATGATTGGATTCAACCCGAATCTTTCCCTTCCTTTAGAGACTCCAATATTCTTCTCTCCGATTCCATCCCCTTAATTGACCTTTCTCTCCCCAATGCCCCTGAGCTCATTACCAATGCATTACGAACTTGGGGGGTATTCCAGGTCATTAACCATGGCGTCCCCATGTCCCTCATCCACTCCATGGAATCCACTGTCAATACCCTCTTTGATCTTCCCTCTTCCCAGAAGCTTAAGGCTGCTCGTTCCCCCGACGGCGTCGCTGGCTACGGCCTTGTCCGTATTTCCCCCTTCTTCACTAAGCGTATGTGGTCTGAAGGCTTCACCATCAACGGTTCCCCTCTTCAACACTTCCAAAAACTCTGGCCTCATGACTACACCAAATACTGGTATTACTATTACTATCACGAACTGTGA

Protein sequence

MAANPKMSDVFKTHPVHVPTIKNLDSFQQLPDTYDWIQPESFPSFRDSNILLSDSIPLIDLSLPNAPELITNALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAARSPDGVAGYGLVRISPFFTKRMWSEGFTINGSPLQHFQKLWPHDYTKYWYYYYYHEL

Homology

[Close]

BLAST of Bhi09G000071 vs. Swiss-Prot
Match: sp|O24648|G3OX_PEA (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 4.6e-43
Identity = 83/162 (51.23%), Postives = 116/162 (71.60%), Query Frame = 0


Query: 5   PKMSDVFKTHPVHV-PTIKNLDSFQQLPDTYDWIQPESFPSFRDSNILLSDS---IPLID 64
           P +S+ ++ HPVHV     + +S Q+LP++Y+W   +   +  DSN ++ +S   +P+ID
Sbjct: 2   PSLSEAYRAHPVHVNHKHPDFNSLQELPESYNWTHLDDH-TLIDSNNIMKESTTTVPVID 61

Query: 65  LSLPNAPELITNALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAARSPDGV 124
           L+ PNA +LI  A +TWGV+QV+NHG+P+SL+  ++    TLF LPS QK KA RSPDGV
Sbjct: 62  LNDPNASKLIGLACKTWGVYQVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHKATRSPDGV 121

Query: 125 AGYGLVRISPFFTKRMWSEGFTINGSPLQHFQKLWPHDYTKY 163
           +GYG+ RIS FF K MW EGFTI GSPL HF++LWP DYT++
Sbjct: 122 SGYGIARISSFFPKLMWYEGFTIVGSPLDHFRELWPQDYTRF 162

[Close]

BLAST of Bhi09G000071 vs. Swiss-Prot
Match: sp|Q39103|G3OX1_ARATH (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 161.0 bits (406), Expect = 1.2e-38
Identity = 77/159 (48.43%), Postives = 104/159 (65.41%), Query Frame = 0


Query: 7   MSDVFKTHPVHVP--TIKNLDSFQQLPDTYDWIQPESFP-SFRDSNILLSDSIPLIDLSL 66
           ++DVF+ HP+H+P   I +  S ++LPD+Y W   +    S   S     ++IPLIDL  
Sbjct: 5   LTDVFRGHPIHLPHSHIPDFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDH 64

Query: 67  PNAPELITNALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAARSPDGVAGY 126
           P+A   I +A RTWG FQ+ NHGVP+ L+  +E    +LF LP  +KLK+ARS  GV+GY
Sbjct: 65  PDATNQIGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGY 124

Query: 127 GLVRISPFFTKRMWSEGFTINGSPLQHFQKLWPHDYTKY 163
           G+ RI+ FF K+MWSEGFTI GSPL  F+KLWP  +  Y
Sbjct: 125 GVARIASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNY 163

[Close]

BLAST of Bhi09G000071 vs. Swiss-Prot
Match: sp|Q9ZT84|G3OX2_ARATH (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 159.8 bits (403), Expect = 2.6e-38
Identity = 74/156 (47.44%), Postives = 104/156 (66.67%), Query Frame = 0


Query: 7   MSDVFKTHPVHVPTIKNLDSFQQLPDTYDWIQPESFPSFRDSNILLSDSIPLIDLSLPNA 66
           +SDVF++HP+H+P + N   F+ LPD+Y W   +       ++    +++PLIDLS  + 
Sbjct: 5   LSDVFRSHPIHIP-LSNPPDFKSLPDSYTWTPKDDLLFSASAS---DETLPLIDLSDIHV 64

Query: 67  PELITNALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAARSPDGVAGYGLV 126
             L+ +A  TWG FQ+ NHGVP  L+  +E    +LF LP  +KLKAARS +GV+GYG+ 
Sbjct: 65  ATLVGHACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYGVA 124

Query: 127 RISPFFTKRMWSEGFTINGSPLQHFQKLWPHDYTKY 163
           RI+ FF K+MWSEGFT+ GSPL  F+KLWP  + KY
Sbjct: 125 RIASFFNKKMWSEGFTVIGSPLHDFRKLWPSHHLKY 156

[Close]

BLAST of Bhi09G000071 vs. Swiss-Prot
Match: sp|Q9C971|G3OX4_ARATH (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 6.0e-35
Identity = 76/151 (50.33%), Postives = 98/151 (64.90%), Query Frame = 0


Query: 21  IKNLDSFQQLPDTYDW--------IQPESFPSFRDSNILLSDSIPLIDLSLPNAPELITN 80
           I NL S Q LP+++ W        ++P S  SF      + +SIP+IDLS P+   LI +
Sbjct: 10  IGNLGSLQTLPESFTWKLTAADSLLRPSSAVSFD----AVEESIPVIDLSNPDVTTLIGD 69

Query: 81  ALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAARSPDGVAGYGLVRISPFF 140
           A +TWG FQ+ NHG+   L+  +ES   TLFD+PS +KL+AA S  GV+GYG  RISPFF
Sbjct: 70  ASKTWGAFQIANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFF 129

Query: 141 TKRMWSEGFTI-NGSPLQHFQKLWPHDYTKY 163
            K+MWSEGFTI + S   HF  LWPHD+TKY
Sbjct: 130 EKKMWSEGFTIADDSYRNHFNTLWPHDHTKY 156

[Close]

BLAST of Bhi09G000071 vs. Swiss-Prot
Match: sp|Q9SVS8|G3OX3_ARATH (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 4.6e-27
Identity = 65/159 (40.88%), Postives = 95/159 (59.75%), Query Frame = 0


Query: 7   MSDVFKTHPVHVPTIKNLD--SFQQLPDTYDWIQPESFPSFRDSNILLSDSIPLIDLSLP 66
           ++ +FK +PV+   I  LD  + + LPD++ W +PE   +        S  IP+I LS P
Sbjct: 4   VTQLFKNNPVNRDRIIPLDFTNTKTLPDSHVWSKPEPETT--------SGPIPVISLSNP 63

Query: 67  NAPELITNALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAARSPDGVAGYG 126
               L+  A   WGVF + +HGV  SL+H+++  +  LF LP  +K+ A RSPD   GYG
Sbjct: 64  EEHGLLRQACEEWGVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYG 123

Query: 127 LVRISPFFTKRMWSEGFTINGSPL-QHFQKLWPHDYTKY 163
           +VRIS F+ K MWSEGF++ GS L +H   LWP D+ ++
Sbjct: 124 VVRISMFYDKLMWSEGFSVMGSSLRRHATLLWPDDHAEF 154

[Close]

BLAST of Bhi09G000071 vs. TAIR10
Match: AT1G15550.1 (gibberellin 3-oxidase 1)

HSP 1 Score: 161.0 bits (406), Expect = 6.5e-40
Identity = 77/159 (48.43%), Postives = 104/159 (65.41%), Query Frame = 0


Query: 7   MSDVFKTHPVHVP--TIKNLDSFQQLPDTYDWIQPESFP-SFRDSNILLSDSIPLIDLSL 66
           ++DVF+ HP+H+P   I +  S ++LPD+Y W   +    S   S     ++IPLIDL  
Sbjct: 5   LTDVFRGHPIHLPHSHIPDFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDH 64

Query: 67  PNAPELITNALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAARSPDGVAGY 126
           P+A   I +A RTWG FQ+ NHGVP+ L+  +E    +LF LP  +KLK+ARS  GV+GY
Sbjct: 65  PDATNQIGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGY 124

Query: 127 GLVRISPFFTKRMWSEGFTINGSPLQHFQKLWPHDYTKY 163
           G+ RI+ FF K+MWSEGFTI GSPL  F+KLWP  +  Y
Sbjct: 125 GVARIASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNY 163

[Close]

BLAST of Bhi09G000071 vs. TAIR10
Match: AT1G80340.1 (gibberellin 3-oxidase 2)

HSP 1 Score: 159.8 bits (403), Expect = 1.4e-39
Identity = 74/156 (47.44%), Postives = 104/156 (66.67%), Query Frame = 0


Query: 7   MSDVFKTHPVHVPTIKNLDSFQQLPDTYDWIQPESFPSFRDSNILLSDSIPLIDLSLPNA 66
           +SDVF++HP+H+P + N   F+ LPD+Y W   +       ++    +++PLIDLS  + 
Sbjct: 5   LSDVFRSHPIHIP-LSNPPDFKSLPDSYTWTPKDDLLFSASAS---DETLPLIDLSDIHV 64

Query: 67  PELITNALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAARSPDGVAGYGLV 126
             L+ +A  TWG FQ+ NHGVP  L+  +E    +LF LP  +KLKAARS +GV+GYG+ 
Sbjct: 65  ATLVGHACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYGVA 124

Query: 127 RISPFFTKRMWSEGFTINGSPLQHFQKLWPHDYTKY 163
           RI+ FF K+MWSEGFT+ GSPL  F+KLWP  + KY
Sbjct: 125 RIASFFNKKMWSEGFTVIGSPLHDFRKLWPSHHLKY 156

[Close]

BLAST of Bhi09G000071 vs. TAIR10
Match: AT1G80330.1 (gibberellin 3-oxidase 4)

HSP 1 Score: 148.7 bits (374), Expect = 3.3e-36
Identity = 76/151 (50.33%), Postives = 98/151 (64.90%), Query Frame = 0


Query: 21  IKNLDSFQQLPDTYDW--------IQPESFPSFRDSNILLSDSIPLIDLSLPNAPELITN 80
           I NL S Q LP+++ W        ++P S  SF      + +SIP+IDLS P+   LI +
Sbjct: 10  IGNLGSLQTLPESFTWKLTAADSLLRPSSAVSFD----AVEESIPVIDLSNPDVTTLIGD 69

Query: 81  ALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAARSPDGVAGYGLVRISPFF 140
           A +TWG FQ+ NHG+   L+  +ES   TLFD+PS +KL+AA S  GV+GYG  RISPFF
Sbjct: 70  ASKTWGAFQIANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFF 129

Query: 141 TKRMWSEGFTI-NGSPLQHFQKLWPHDYTKY 163
            K+MWSEGFTI + S   HF  LWPHD+TKY
Sbjct: 130 EKKMWSEGFTIADDSYRNHFNTLWPHDHTKY 156

[Close]

BLAST of Bhi09G000071 vs. TAIR10
Match: AT4G21690.1 (gibberellin 3-oxidase 3)

HSP 1 Score: 122.5 bits (306), Expect = 2.6e-28
Identity = 65/159 (40.88%), Postives = 95/159 (59.75%), Query Frame = 0


Query: 7   MSDVFKTHPVHVPTIKNLD--SFQQLPDTYDWIQPESFPSFRDSNILLSDSIPLIDLSLP 66
           ++ +FK +PV+   I  LD  + + LPD++ W +PE   +        S  IP+I LS P
Sbjct: 4   VTQLFKNNPVNRDRIIPLDFTNTKTLPDSHVWSKPEPETT--------SGPIPVISLSNP 63

Query: 67  NAPELITNALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAARSPDGVAGYG 126
               L+  A   WGVF + +HGV  SL+H+++  +  LF LP  +K+ A RSPD   GYG
Sbjct: 64  EEHGLLRQACEEWGVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYG 123

Query: 127 LVRISPFFTKRMWSEGFTINGSPL-QHFQKLWPHDYTKY 163
           +VRIS F+ K MWSEGF++ GS L +H   LWP D+ ++
Sbjct: 124 VVRISMFYDKLMWSEGFSVMGSSLRRHATLLWPDDHAEF 154

[Close]

BLAST of Bhi09G000071 vs. TAIR10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 67.8 bits (164), Expect = 7.5e-12
Identity = 39/115 (33.91%), Postives = 53/115 (46.09%), Query Frame = 0


Query: 52  LSDSIPLIDLSLPNAPE---------LITNALRTWGVFQVINHGVPMSLIHSMESTVNTL 111
           L   IP+IDLS  + P+          ++ A   WG FQVINHG+ + ++  +E   +  
Sbjct: 51  LHHQIPVIDLSKLSKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEF 110

Query: 112 FDLPSSQKLKAARSPDGVAGYGLVRISPFFTKRMWSEGFTINGSPLQ-HFQKLWP 157
           FD+P  +K K    P  V GYG   I     K  W   F +   P Q    KLWP
Sbjct: 111 FDMPLEEKKKYPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWP 165

[Close]

BLAST of Bhi09G000071 vs. TrEMBL
Match: tr|S0BE27|S0BE27_CUCSA (GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox3 PE=2 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.4e-68
Identity = 124/159 (77.99%), Postives = 142/159 (89.31%), Query Frame = 0


Query: 5   PKMSDVFKTHPVHVPTIK-NLDSFQQLPDTYDWIQPESFPSFRDSNILLSDSIPLIDLSL 64
           PK++D++KTHP+ +PT K NLDS QQLPDTYDWIQP++FPSFRD NI LSDS+PLIDLSL
Sbjct: 7   PKLTDIYKTHPLDIPTNKLNLDSLQQLPDTYDWIQPDAFPSFRDPNISLSDSVPLIDLSL 66

Query: 65  PNAPELITNALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAARSPDGVAGY 124
           PNAP LI NAL+TWGVFQVINHGVP+SL++SMES  NTLFDLPS  KLKAAR+PDGV GY
Sbjct: 67  PNAPHLIGNALKTWGVFQVINHGVPISLLNSMESFTNTLFDLPSPHKLKAARTPDGVTGY 126

Query: 125 GLVRISPFFTKRMWSEGFTINGSPLQHFQKLWPHDYTKY 163
           GL RIS FF KRMWSEGFTI GSPL+HFQKLWP++Y+KY
Sbjct: 127 GLPRISTFFPKRMWSEGFTIVGSPLEHFQKLWPNEYSKY 165

[Close]

BLAST of Bhi09G000071 vs. TrEMBL
Match: tr|A0A1S3CF23|A0A1S3CF23_CUCME (LOW QUALITY PROTEIN: gibberellin 3-beta-dioxygenase 2-like OS=Cucumis melo OX=3656 GN=LOC103499757 PE=3 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 4.1e-65
Identity = 121/160 (75.62%), Postives = 139/160 (86.88%), Query Frame = 0


Query: 5   PKMSDVFKTHPVHVPTIKNLDSFQQLPDTYDWIQPESFP-SFRDSNILLSDSIPLIDLS- 64
           PK++DV+KTHP+ +PT KNLDSF++LPDTYDWIQP+SFP S  D N+ LSDS+PLIDLS 
Sbjct: 5   PKLTDVYKTHPLDIPTNKNLDSFEKLPDTYDWIQPDSFPSSLTDPNVSLSDSVPLIDLSD 64

Query: 65  LPNAPELITNALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAARSPDGVAG 124
           LPNAP LI NAL+TWG FQVINHGVP+SL++SMES  N LFDLPS  KLKAAR+PDGVAG
Sbjct: 65  LPNAPHLIGNALKTWGXFQVINHGVPISLLNSMESFTNALFDLPSPHKLKAARTPDGVAG 124

Query: 125 YGLVRISPFFTKRMWSEGFTINGSPLQHFQKLWPHDYTKY 163
           YGL RIS FF KRMWSEGFTI GSPL+HFQKLWP +Y+KY
Sbjct: 125 YGLPRISTFFPKRMWSEGFTIVGSPLEHFQKLWPDEYSKY 164

[Close]

BLAST of Bhi09G000071 vs. TrEMBL
Match: tr|K7ZTU0|K7ZTU0_CUCSA (Gibberellin 3-oxidase (Fragment) OS=Cucumis sativus OX=3659 GN=ga3ox1 PE=2 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 6.0e-64
Identity = 118/164 (71.95%), Postives = 138/164 (84.15%), Query Frame = 0


Query: 1   MAANPKMSDVFKTHPVHVPTIKNL--DSFQQLPDTYDWIQPESFPSFRDSNILLSDSIPL 60
           M +N K+++VFK+HPVH+P  KNL  DS  +LPD+YDWIQP+SFPS   SN  LSDSIPL
Sbjct: 1   MGSNIKITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPL 60

Query: 61  IDLSLPNAPELITNALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAARSPD 120
           IDLSLPNAP+LI NA RTWG FQVINHGVP+SL+HS+ES+ N+LF LP   KLKAAR  D
Sbjct: 61  IDLSLPNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSD 120

Query: 121 GVAGYGLVRISPFFTKRMWSEGFTINGSPLQHFQKLWPHDYTKY 163
           G++GYGLVRIS FF KRMWSEGFTI GSPL+HF+KLWPHDYT+Y
Sbjct: 121 GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRY 164

[Close]

BLAST of Bhi09G000071 vs. TrEMBL
Match: tr|A0A1S3CDD3|A0A1S3CDD3_CUCME (gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103499758 PE=3 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.0e-63
Identity = 117/164 (71.34%), Postives = 138/164 (84.15%), Query Frame = 0


Query: 1   MAANPKMSDVFKTHPVHVPTIKN--LDSFQQLPDTYDWIQPESFPSFRDSNILLSDSIPL 60
           M +N K+++VFK+HPVH+P  KN   DS Q+LPD+YDWIQP+SFP+   SN  LSDSIPL
Sbjct: 1   MGSNIKITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHLSDSIPL 60

Query: 61  IDLSLPNAPELITNALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAARSPD 120
           IDLSLPNAP LI NA RTWG FQVINHGVP+SL+HS+ES+ N+LF LP+  KLKAAR  D
Sbjct: 61  IDLSLPNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSD 120

Query: 121 GVAGYGLVRISPFFTKRMWSEGFTINGSPLQHFQKLWPHDYTKY 163
           G++GYGLVRIS FF KRMWSEGFTI GSPL+HF+KLWPHDYT+Y
Sbjct: 121 GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRY 164

[Close]

BLAST of Bhi09G000071 vs. TrEMBL
Match: tr|A0A1S3CEM3|A0A1S3CEM3_CUCME (gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103500015 PE=3 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.5e-62
Identity = 113/168 (67.26%), Postives = 141/168 (83.93%), Query Frame = 0


Query: 1   MAANPKMSDVFKTHPVHVPTIKNLDSFQQLPDTYDWIQPESFPSFRD------SNILLSD 60
           MA  PK+++ ++T P+H  T KNLDSF+++PDT+DW+QP SFPSFRD      S++  +D
Sbjct: 1   MATIPKITEAYRTDPIHAITNKNLDSFEEIPDTHDWVQPNSFPSFRDDHSNNISDLPDTD 60

Query: 61  SIPLIDLSLPNAPELITNALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAA 120
           S+PLIDLSLPNAP+LI NALRTWGVFQVINHGVP+S+++SME+ ++ LFDLP+ QKLKAA
Sbjct: 61  SVPLIDLSLPNAPKLIGNALRTWGVFQVINHGVPISVLNSMETLLHDLFDLPTPQKLKAA 120

Query: 121 RSPDGVAGYGLVRISPFFTKRMWSEGFTINGSPLQHFQKLWPHDYTKY 163
           R+PDGV GYG  RISPFF K MWSEGFT++GSPL+HFQ LWPHD TKY
Sbjct: 121 RAPDGVNGYGRFRISPFFPKSMWSEGFTVSGSPLEHFQILWPHDCTKY 168

[Close]

BLAST of Bhi09G000071 vs. NCBI nr
Match: NP_001267625.1 (gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus] >CCD28484.1 GA 3-oxidase [Cucumis sativus] >KGN45325.1 hypothetical protein Csa_7G435500 [Cucumis sativus])

HSP 1 Score: 268.1 bits (684), Expect = 2.1e-68
Identity = 124/159 (77.99%), Postives = 142/159 (89.31%), Query Frame = 0


Query: 5   PKMSDVFKTHPVHVPTIK-NLDSFQQLPDTYDWIQPESFPSFRDSNILLSDSIPLIDLSL 64
           PK++D++KTHP+ +PT K NLDS QQLPDTYDWIQP++FPSFRD NI LSDS+PLIDLSL
Sbjct: 7   PKLTDIYKTHPLDIPTNKLNLDSLQQLPDTYDWIQPDAFPSFRDPNISLSDSVPLIDLSL 66

Query: 65  PNAPELITNALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAARSPDGVAGY 124
           PNAP LI NAL+TWGVFQVINHGVP+SL++SMES  NTLFDLPS  KLKAAR+PDGV GY
Sbjct: 67  PNAPHLIGNALKTWGVFQVINHGVPISLLNSMESFTNTLFDLPSPHKLKAARTPDGVTGY 126

Query: 125 GLVRISPFFTKRMWSEGFTINGSPLQHFQKLWPHDYTKY 163
           GL RIS FF KRMWSEGFTI GSPL+HFQKLWP++Y+KY
Sbjct: 127 GLPRISTFFPKRMWSEGFTIVGSPLEHFQKLWPNEYSKY 165

[Close]

BLAST of Bhi09G000071 vs. NCBI nr
Match: XP_008461055.1 (PREDICTED: LOW QUALITY PROTEIN: gibberellin 3-beta-dioxygenase 2-like [Cucumis melo])

HSP 1 Score: 256.5 bits (654), Expect = 6.3e-65
Identity = 121/160 (75.62%), Postives = 139/160 (86.88%), Query Frame = 0


Query: 5   PKMSDVFKTHPVHVPTIKNLDSFQQLPDTYDWIQPESFP-SFRDSNILLSDSIPLIDLS- 64
           PK++DV+KTHP+ +PT KNLDSF++LPDTYDWIQP+SFP S  D N+ LSDS+PLIDLS 
Sbjct: 5   PKLTDVYKTHPLDIPTNKNLDSFEKLPDTYDWIQPDSFPSSLTDPNVSLSDSVPLIDLSD 64

Query: 65  LPNAPELITNALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAARSPDGVAG 124
           LPNAP LI NAL+TWG FQVINHGVP+SL++SMES  N LFDLPS  KLKAAR+PDGVAG
Sbjct: 65  LPNAPHLIGNALKTWGXFQVINHGVPISLLNSMESFTNALFDLPSPHKLKAARTPDGVAG 124

Query: 125 YGLVRISPFFTKRMWSEGFTINGSPLQHFQKLWPHDYTKY 163
           YGL RIS FF KRMWSEGFTI GSPL+HFQKLWP +Y+KY
Sbjct: 125 YGLPRISTFFPKRMWSEGFTIVGSPLEHFQKLWPDEYSKY 164

[Close]

BLAST of Bhi09G000071 vs. NCBI nr
Match: XP_004135830.1 (PREDICTED: gibberellin 3-beta-dioxygenase 1 [Cucumis sativus] >CBX88045.1 gibberellin 3-oxidase, partial [Cucumis sativus] >KGN45321.1 hypothetical protein Csa_7G434970 [Cucumis sativus])

HSP 1 Score: 252.7 bits (644), Expect = 9.1e-64
Identity = 118/164 (71.95%), Postives = 138/164 (84.15%), Query Frame = 0


Query: 1   MAANPKMSDVFKTHPVHVPTIKNL--DSFQQLPDTYDWIQPESFPSFRDSNILLSDSIPL 60
           M +N K+++VFK+HPVH+P  KNL  DS  +LPD+YDWIQP+SFPS   SN  LSDSIPL
Sbjct: 1   MGSNIKITEVFKSHPVHIPIHKNLDFDSLHELPDSYDWIQPDSFPSSSLSNNHLSDSIPL 60

Query: 61  IDLSLPNAPELITNALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAARSPD 120
           IDLSLPNAP+LI NA RTWG FQVINHGVP+SL+HS+ES+ N+LF LP   KLKAAR  D
Sbjct: 61  IDLSLPNAPQLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPPPHKLKAARPSD 120

Query: 121 GVAGYGLVRISPFFTKRMWSEGFTINGSPLQHFQKLWPHDYTKY 163
           G++GYGLVRIS FF KRMWSEGFTI GSPL+HF+KLWPHDYT+Y
Sbjct: 121 GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRY 164

[Close]

BLAST of Bhi09G000071 vs. NCBI nr
Match: XP_008461056.1 (PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo])

HSP 1 Score: 251.9 bits (642), Expect = 1.5e-63
Identity = 117/164 (71.34%), Postives = 138/164 (84.15%), Query Frame = 0


Query: 1   MAANPKMSDVFKTHPVHVPTIKN--LDSFQQLPDTYDWIQPESFPSFRDSNILLSDSIPL 60
           M +N K+++VFK+HPVH+P  KN   DS Q+LPD+YDWIQP+SFP+   SN  LSDSIPL
Sbjct: 1   MGSNIKITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHLSDSIPL 60

Query: 61  IDLSLPNAPELITNALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAARSPD 120
           IDLSLPNAP LI NA RTWG FQVINHGVP+SL+HS+ES+ N+LF LP+  KLKAAR  D
Sbjct: 61  IDLSLPNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSD 120

Query: 121 GVAGYGLVRISPFFTKRMWSEGFTINGSPLQHFQKLWPHDYTKY 163
           G++GYGLVRIS FF KRMWSEGFTI GSPL+HF+KLWPHDYT+Y
Sbjct: 121 GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRY 164

[Close]

BLAST of Bhi09G000071 vs. NCBI nr
Match: XP_008461419.1 (PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo])

HSP 1 Score: 247.3 bits (630), Expect = 3.8e-62
Identity = 113/168 (67.26%), Postives = 141/168 (83.93%), Query Frame = 0


Query: 1   MAANPKMSDVFKTHPVHVPTIKNLDSFQQLPDTYDWIQPESFPSFRD------SNILLSD 60
           MA  PK+++ ++T P+H  T KNLDSF+++PDT+DW+QP SFPSFRD      S++  +D
Sbjct: 1   MATIPKITEAYRTDPIHAITNKNLDSFEEIPDTHDWVQPNSFPSFRDDHSNNISDLPDTD 60

Query: 61  SIPLIDLSLPNAPELITNALRTWGVFQVINHGVPMSLIHSMESTVNTLFDLPSSQKLKAA 120
           S+PLIDLSLPNAP+LI NALRTWGVFQVINHGVP+S+++SME+ ++ LFDLP+ QKLKAA
Sbjct: 61  SVPLIDLSLPNAPKLIGNALRTWGVFQVINHGVPISVLNSMETLLHDLFDLPTPQKLKAA 120

Query: 121 RSPDGVAGYGLVRISPFFTKRMWSEGFTINGSPLQHFQKLWPHDYTKY 163
           R+PDGV GYG  RISPFF K MWSEGFT++GSPL+HFQ LWPHD TKY
Sbjct: 121 RAPDGVNGYGRFRISPFFPKSMWSEGFTVSGSPLEHFQILWPHDCTKY 168

The following BLAST results are available for this feature:

BLAST of Bhi09G000071 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O24648\|G3OX_PEA	4.6e-43	51.23	Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1	[more]
sp\|Q39103\|G3OX1_ARATH	1.2e-38	48.43	Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 ...	[more]
sp\|Q9ZT84\|G3OX2_ARATH	2.6e-38	47.44	Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 ...	[more]
sp\|Q9C971\|G3OX4_ARATH	6.0e-35	50.33	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ...	[more]
sp\|Q9SVS8\|G3OX3_ARATH	4.6e-27	40.88	Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 ...	[more]

BLAST of Bhi09G000071 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G15550.1	6.5e-40	48.43	gibberellin 3-oxidase 1	[more]
AT1G80340.1	1.4e-39	47.44	gibberellin 3-oxidase 2	[more]
AT1G80330.1	3.3e-36	50.33	gibberellin 3-oxidase 4	[more]
AT4G21690.1	2.6e-28	40.88	gibberellin 3-oxidase 3	[more]
AT3G21420.1	7.5e-12	33.91	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

BLAST of Bhi09G000071 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|S0BE27\|S0BE27_CUCSA	1.4e-68	77.99	GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox3 PE=2 SV=1	[more]
tr\|A0A1S3CF23\|A0A1S3CF23_CUCME	4.1e-65	75.63	LOW QUALITY PROTEIN: gibberellin 3-beta-dioxygenase 2-like OS=Cucumis melo OX=36...	[more]
tr\|K7ZTU0\|K7ZTU0_CUCSA	6.0e-64	71.95	Gibberellin 3-oxidase (Fragment) OS=Cucumis sativus OX=3659 GN=ga3ox1 PE=2 SV=1	[more]
tr\|A0A1S3CDD3\|A0A1S3CDD3_CUCME	1.0e-63	71.34	gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103499758 PE...	[more]
tr\|A0A1S3CEM3\|A0A1S3CEM3_CUCME	2.5e-62	67.26	gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103500015 PE...	[more]

BLAST of Bhi09G000071 vs. NCBI nr


Match Name	E-value	Identity	Description
NP_001267625.1	2.1e-68	77.99	gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus] >CCD28484.1 GA 3-oxidase...	[more]
XP_008461055.1	6.3e-65	75.63	PREDICTED: LOW QUALITY PROTEIN: gibberellin 3-beta-dioxygenase 2-like [Cucumis m...	[more]
XP_004135830.1	9.1e-64	71.95	PREDICTED: gibberellin 3-beta-dioxygenase 1 [Cucumis sativus] >CBX88045.1 gibber...	[more]
XP_008461056.1	1.5e-63	71.34	PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo]	[more]
XP_008461419.1	3.8e-62	67.26	PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR026992	DIOX_N
IPR027443	IPNS-like

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009686	gibberellin biosynthetic process
biological_process	GO:0055114	oxidation-reduction process
molecular_function	GO:0016707	gibberellin 3-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi09M000071	Bhi09M000071	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 12..171 e-value: 1.0E-20 score: 76.5
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 56..156 e-value: 1.4E-18 score: 67.7
None	No IPR available	PANTHER	PTHR10209:SF95	GIBBERELLIN 3-BETA-DIOXYGENASE 1-RELATED	coord: 21..163
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 21..163
None	No IPR available	SUPERFAMILY	SSF51197	Clavaminate synthase-like	coord: 26..163

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi09G000071	Cucumber (Gy14) v1	cgywgoB051
Bhi09G000071	Cucumber (Gy14) v1	cgywgoB327
Bhi09G000071	Cucumber (Gy14) v2	cgybwgoB198
Bhi09G000071	Cucumber (Gy14) v2	cgybwgoB659
Bhi09G000071	Cucurbita maxima (Rimu)	cmawgoB0492
Bhi09G000071	Cucurbita maxima (Rimu)	cmawgoB0626
Bhi09G000071	Cucurbita maxima (Rimu)	cmawgoB1132
Bhi09G000071	Cucurbita moschata (Rifu)	cmowgoB0491
Bhi09G000071	Cucurbita moschata (Rifu)	cmowgoB0625
Bhi09G000071	Cucurbita moschata (Rifu)	cmowgoB1130
Bhi09G000071	Cucurbita pepo (Zucchini)	cpewgoB0208
Bhi09G000071	Cucurbita pepo (Zucchini)	cpewgoB0417
Bhi09G000071	Cucurbita pepo (Zucchini)	cpewgoB0721
Bhi09G000071	Wild cucumber (PI 183967)	cpiwgoB208
Bhi09G000071	Wild cucumber (PI 183967)	cpiwgoB702
Bhi09G000071	Cucumber (Chinese Long) v3	cucwgoB214
Bhi09G000071	Cucumber (Chinese Long) v3	cucwgoB715
Bhi09G000071	Cucumber (Chinese Long) v2	cuwgoB208
Bhi09G000071	Cucumber (Chinese Long) v2	cuwgoB690
Bhi09G000071	Bottle gourd (USVL1VR-Ls)	lsiwgoB270
Bhi09G000071	Bottle gourd (USVL1VR-Ls)	lsiwgoB577
Bhi09G000071	Melon (DHL92) v3.6.1	medwgoB240
Bhi09G000071	Melon (DHL92) v3.5.1	mewgoB239
Bhi09G000071	Melon (DHL92) v3.5.1	mewgoB368
Bhi09G000071	Watermelon (Charleston Gray)	wcgwgoB068
Bhi09G000071	Watermelon (Charleston Gray)	wcgwgoB599
Bhi09G000071	Watermelon (Charleston Gray)	wcgwgoB659
Bhi09G000071	Watermelon (97103) v2	wgowmbB594
Bhi09G000071	Watermelon (97103) v2	wgowmbB607
Bhi09G000071	Watermelon (97103) v2	wgowmbB614
Bhi09G000071	Watermelon (97103) v1	wgowmB585
Bhi09G000071	Watermelon (97103) v1	wgowmB604
Bhi09G000071	Watermelon (97103) v1	wgowmB614
Bhi09G000071	Silver-seed gourd	carwgoB0150
Bhi09G000071	Silver-seed gourd	carwgoB0184
Bhi09G000071	Silver-seed gourd	carwgoB0253
Bhi09G000071	Silver-seed gourd	carwgoB1204