Bhi09G000063 (gene) Wax gourd

Overview
NameBhi09G000063
Typegene
OrganismBenincasa hispida (Wax gourd)
Description2-oxoglutarate-dependent dioxygenase, putative
Locationchr9 : 1307629 .. 1310067 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAATTAAAGCACCTACCTTTTTTCTTTCTTTGATAATTATTTTTAGAACTTGTTTTTCTCTTACATAAGCCATCAAAGAAAAATGAAAAAAGGTAGCTGAGTGAGTTAGGTTGTATGAGAATGGTTAATTCTGAAGCAAAAGCCAAAGTACTTCCCATAATAGATTTGTCAAAATTAGAGGCAGTTGGCACAGAAGAATGGGGTTCAGCGTGCAAAGAGATGAGGCTTGGATTAGAAGAATTTGGTTGTTTCTCAGTGGTTTATGACAAAGTTTCTTTGGAACTTCATAACTCCATTTTTGAAGCAACAAAAGAGCTTTTTCATCTCCCATTAGAAACTAGAACTAAGAACACTAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCACTTCTTCCTCTCTATGAATCAATGGCTATTGATAATCCAACTGAGCTACAAACAACACAGCTGAGTTTGGCCAATCTCCTGTGGCCTTCTAGAGCAAACAATCACTTCTGGTAATTAATTTAATTAACACACACTACTATTCTTGTCATCTATTTCATCCTTCTTATAGTTTTAAAAGTTTTGATTCATATGATACTAATTATTTAGTTTCAATTTGATTCTTATGAATTTGTGTCATTAAACCAAACCCACAAATAATTGATTCCTTAATGATTTGTTTAGATCTTCATATCCGAAGTGGTATGCTATAAACTTCTTGTTGTTGCTCATTGCTTTCTAGCCTATTACAATCTTTCTCTTCTCTGTTCATACATTCAAAACGCTTGCGAAAACCTTTTTCTCCAAAGCCGTTTTCTCTAAAACGAGGGTGGATGTTGCGAGAGGCACACGGTGTGCCATCGGCATTCTGTATTCGAATTGGCTGATTTGTTTGTGAGCGGAAACAAGTACCACAAAATCGCTAAGTGAAGCTTCTGAAAATGCGATTGTGACAGTTATATCACATGTGTGAGAGTGAAGATTTAATACTTACCATCTCAAATTGATAAAGTTTCTTATGGCCTGTTTGGATCACAAATATTGTTTCATAGGTATAAATATCAAATATTTGTAATTTAAATGTGTGAATTTTAAATGTTTGAGATAATTAATGTATGTATTTAGATATGCATCATCATTAATATTTAGAAGTTAATTATATGTGTTTGATTTACCAGTTTTGCATATAGAAATAAAAATTAAATAATTATTTAGTTAAAGGTTAGAGAACAAGTATGATGGTCATCAATAAAATTTAGCATGATCACTTAAAATATATGTAAATGAAGAATTTTATATTAAACGTTGATTAAATTATTGAATTTAATTACTAAAAACAATGAAATACATATAAATTAATTAGGATTTTAGAATTTTACATTAAATAGTAAGGTAACATAAAAATATTAATTTTAAATGTATTAGTGAAAGATTAATTCTAATATATTTATACTTAATAATTAATTAAAATTAATAGTAAGGATTAATTGATATTAATTTATTAATTAATTAACTTATATTTTTATTGTTCATCTACTACGTATAAAAATTTCACTTGTATGGAGGGTATTTTAAAATTCATGTCATATGATTTTTTAAGTTCTATGGATAATAATATCTTTAGTTTTAAAAAGTGAAAGGTAAAATAGATAAGGATATTAAAAAATGTCATAGCAAAACTTGTAAAATGAATAGACCTTAAAGTTGTAATACTTGTGTGTATTATGAACTACATCCATCTTTATGCAACCCACTCCAATAATATCTAAAAAGTAAAAAAAATGTTTTTTTTAAAATTTGTCATGTGGCAAATCGTGGTTGGACGAACCACCCAAGATGCATATTTTTCTTAATTATATCTTACAAAAAAAAGATTTTTTTTGAGTAATAATGTCATGCATTAAATACGAACATTTAAATTTGTATCTATTTGGTTAAACTTTAAAAGGTGTTTAATACCTTCTTCTTTTAATAGAACTATTATATATGAAATTGAATATTATAGTTAATAGAACTTATAAATAATAAAATTGACCTATTAAATATAGAGTAACTGAGAAAGATGAAACCCATACGGGTTTGAATTACCATACAGATCTCACTTTCTTGTCCATAATTCACCAGCATCTCATTTCTGGTCTTCAAATTCAATTGCTTGATGATCAATGGCTCCATGTTCAACCCTCAACCCATTGCCCCTTCATTGTCATGGCTGGCGATGTACTCATGGTAACCACACAGTCACACCAATCCTTTTTTTTTTTTTGTTTCTTCATAGCAAAATTTAGTCCTTAAACTTTTAAGACCTAATCGGATCACACCAATCCCTTTTTTTGTTTTTCCATATCAAAATTTAGTCCTTAAACTTTTTAAACCTAATCGGAATAGATCTTTTAACCATAAAACATGAGTATGGTAAAAGAAATATGTTTGTCTTGTGAAACC

mRNA sequence

AAAAAATTAAAGCACCTACCTTTTTTCTTTCTTTGATAATTATTTTTAGAACTTGTTTTTCTCTTACATAAGCCATCAAAGAAAAATGAAAAAAGGTAGCTGAGTGAGTTAGGTTGTATGAGAATGGTTAATTCTGAAGCAAAAGCCAAAGTACTTCCCATAATAGATTTGTCAAAATTAGAGGCAGTTGGCACAGAAGAATGGGGTTCAGCGTGCAAAGAGATGAGGCTTGGATTAGAAGAATTTGGTTGTTTCTCAGTGGTTTATGACAAAGTTTCTTTGGAACTTCATAACTCCATTTTTGAAGCAACAAAAGAGCTTTTTCATCTCCCATTAGAAACTAGAACTAAGAACACTAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCACTTCTTCCTCTCTATGAATCAATGGCTATTGATAATCCAACTGAGCTACAAACAACACAGCTGAGTTTGGCCAATCTCCTGTGGCCTTCTAGAGCAAACAATCACTTCTGCATCTCATTTCTGGTCTTCAAATTCAATTGCTTGATGATCAATGGCTCCATGTTCAACCCTCAACCCATTGCCCCTTCATTGTCATGGCTGGCGATGTACTCATGGTAACCACACAGTCACACCAATCCTTTTTTTTTTTTTGTTTCTTCATAGCAAAATTTAGTCCTTAAACTTTTAAGACCTAATCGGATCACACCAATCCCTTTTTTTGTTTTTCCATATCAAAATTTAGTCCTTAAACTTTTTAAACCTAATCGGAATAGATCTTTTAACCATAAAACATGAGTATGGTAAAAGAAATATGTTTGTCTTGTGAAACC

Coding sequence (CDS)

ATGAGAATGGTTAATTCTGAAGCAAAAGCCAAAGTACTTCCCATAATAGATTTGTCAAAATTAGAGGCAGTTGGCACAGAAGAATGGGGTTCAGCGTGCAAAGAGATGAGGCTTGGATTAGAAGAATTTGGTTGTTTCTCAGTGGTTTATGACAAAGTTTCTTTGGAACTTCATAACTCCATTTTTGAAGCAACAAAAGAGCTTTTTCATCTCCCATTAGAAACTAGAACTAAGAACACTAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCACTTCTTCCTCTCTATGAATCAATGGCTATTGATAATCCAACTGAGCTACAAACAACACAGCTGAGTTTGGCCAATCTCCTGTGGCCTTCTAGAGCAAACAATCACTTCTGCATCTCATTTCTGGTCTTCAAATTCAATTGCTTGATGATCAATGGCTCCATGTTCAACCCTCAACCCATTGCCCCTTCATTGTCATGGCTGGCGATGTACTCATGGTAA

Protein sequence

MRMVNSEAKAKVLPIIDLSKLEAVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATKELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRANNHFCISFLVFKFNCLMINGSMFNPQPIAPSLSWLAMYSW
Homology
[Close]
BLAST of Bhi09G000063 vs. Swiss-Prot
Match: sp|Q945B6|AOP1L_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 3.2e-17
Identity = 44/115 (38.26%), Postives = 65/115 (56.52%), Query Frame = 0 

Query: 13  LPIIDLSKLE-AVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATKELFHL 72
           LP+ID S      G+ +W     ++   LE++GCF   +DK+S+EL+ S+FEA ++LF L
Sbjct: 14  LPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLFEL 73

Query: 73  PLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRAN 127
           P+ T+ +N S KP+HGY        LYES+ ID+   L+         LWP   N
Sbjct: 74  PIPTKQRNVSSKPFHGYL----CHNLYESLGIDDANVLEKVN-DFTQQLWPDHGN 123

[Close]
BLAST of Bhi09G000063 vs. Swiss-Prot
Match: sp|Q9ZTA3|AOP1C_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 9.3e-17
Identity = 43/115 (37.39%), Postives = 65/115 (56.52%), Query Frame = 0 

Query: 13  LPIIDLSKLE-AVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATKELFHL 72
           LP+ID S      G+ +W     ++   LE++GCF   +DK+S+EL+ S+FEA ++LF L
Sbjct: 14  LPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFEL 73

Query: 73  PLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRAN 127
           P+ T+ +N S KP+HGY        LYES+ I++   L+         LWP   N
Sbjct: 74  PIPTKQRNVSSKPFHGYL----CHNLYESLGINDANVLEKVN-DFTQQLWPDHGN 123

[Close]
BLAST of Bhi09G000063 vs. Swiss-Prot
Match: sp|Q945B5|AOP2V_ARATH (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 6.0e-16
Identity = 47/116 (40.52%), Postives = 63/116 (54.31%), Query Frame = 0 

Query: 13  LPIIDLS-KLEAVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATKELFHL 72
           LP+I+L+ K    G+ +W     ++R  LE+FGCF   YDKVSLEL  SI +  +ELF L
Sbjct: 8   LPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELFAL 67

Query: 73  PLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRANN 128
           P+ET+ +N   KPY GY    +   L ES+ I N   L+         LWP    N
Sbjct: 68  PVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENVN-EFTQQLWPDGDGN 119

[Close]
BLAST of Bhi09G000063 vs. Swiss-Prot
Match: sp|Q9ZTA2|AOP2C_ARATH (Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.0e-15
Identity = 47/116 (40.52%), Postives = 63/116 (54.31%), Query Frame = 0 

Query: 13  LPIIDLS-KLEAVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATKELFHL 72
           LP+I+L+ K    G+ +W     ++R  LE+FGCF   YDKVSLEL  SI +  +ELF L
Sbjct: 8   LPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELFAL 67

Query: 73  PLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRANN 128
           P+ET+ +N   KPY GY    +   L ES+ I N   L+         LWP    N
Sbjct: 68  PVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENIN-EFTQQLWPHGDGN 119

[Close]
BLAST of Bhi09G000063 vs. Swiss-Prot
Match: sp|Q944Z5|AOP1V_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.3e-15
Identity = 42/115 (36.52%), Postives = 64/115 (55.65%), Query Frame = 0 

Query: 13  LPIIDLSKLE-AVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATKELFHL 72
           LP+ID S      G+ +W     ++   LE++GCF   +DK+S+EL+ S+FEA ++LF L
Sbjct: 7   LPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFEL 66

Query: 73  PLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRAN 127
           P+ T+ +N S K +HGY        LYES+ I++   L+         LWP   N
Sbjct: 67  PIPTKQRNVSSKLFHGYL----CHNLYESLGINDANVLEKVN-DFTQQLWPDHGN 116

[Close]
BLAST of Bhi09G000063 vs. TAIR10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 107.1 bits (266), Expect = 1.1e-23
Identity = 53/124 (42.74%), Postives = 76/124 (61.29%), Query Frame = 0 

Query: 9   KAKVLPIIDLSKLEAV--GTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATK 68
           K   +PI+DL+  + +   T  W S  +E    LEE+GCF  VYD V+ +L +SIF A +
Sbjct: 6   KTTKVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAE 65

Query: 69  ELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRAN 128
           ELF LP ET+ KN +EKPYHGY G   ++PL+E + +D  T  +  Q    +L+WP + N
Sbjct: 66  ELFDLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQ-RFTHLMWP-QGN 125

Query: 129 NHFC 131
           + FC
Sbjct: 126 DRFC 127

[Close]
BLAST of Bhi09G000063 vs. TAIR10
Match: AT1G52810.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 97.8 bits (242), Expect = 6.5e-21
Identity = 50/128 (39.06%), Postives = 79/128 (61.72%), Query Frame = 0 

Query: 13  LPIIDLSKLE-AVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATKELFHL 72
           LP+ID +  +   GT +W S   ++R  LEE+GCF  ++DKV LEL  ++F+A++E+F L
Sbjct: 12  LPVIDFTSRDLKPGTIQWDSVRADVRRALEEYGCFEALFDKVPLELRKAVFDASEEVFQL 71

Query: 73  PLETRTKNTSEKPYHGYFGGYSLLPLYESMAID---NPTELQTTQLSLANLLWPSRANNH 132
           PLET+ +  S++ Y GY G    LPL+E M +D   NP ++     +  + LWP + NN+
Sbjct: 72  PLETKKRVVSKRKYRGYVGQIPTLPLFEVMGVDFAENPDKVN----AFTHKLWP-QGNNN 131

Query: 133 FCISFLVF 137
           F  + + F
Sbjct: 132 FSEAVMSF 134

[Close]
BLAST of Bhi09G000063 vs. TAIR10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 94.7 bits (234), Expect = 5.5e-20
Identity = 49/119 (41.18%), Postives = 74/119 (62.18%), Query Frame = 0 

Query: 13  LPIIDLSKLE-AVGTEEWGSACKEMRLGLEEFGCFSV-VYDKVSLELHNSIFEATKELFH 72
           +P +D S+ +   GT+ W S  + +R  LEE+GCF + + DK  L+L + +F +  +LF 
Sbjct: 5   IPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDLFD 64

Query: 73  LPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRANNHF 130
           LP +T+ KN  +KP +GY G    LPL+ES+ IDN T L+ T+ S   L+WP + N HF
Sbjct: 65  LPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATR-SFTGLMWP-QGNEHF 121

[Close]
BLAST of Bhi09G000063 vs. TAIR10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 94.4 bits (233), Expect = 7.2e-20
Identity = 45/111 (40.54%), Postives = 69/111 (62.16%), Query Frame = 0 

Query: 13  LPIIDLS-KLEAVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATKELFHL 72
           LP+ID S K    G  EW     +++  L+++G F   +D++  EL  S+F A +ELF L
Sbjct: 12  LPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELFDL 71

Query: 73  PLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWP 123
           PL+T+ +N S+KP+HGY G Y ++PLYESM ID+ +++     +    LWP
Sbjct: 72  PLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDD-SDIAEKVDAFTEKLWP 121

[Close]
BLAST of Bhi09G000063 vs. TAIR10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 88.2 bits (217), Expect = 5.2e-18
Identity = 43/115 (37.39%), Postives = 65/115 (56.52%), Query Frame = 0 

Query: 13  LPIIDLSKLE-AVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEATKELFHL 72
           LP+ID S      G+ +W     ++   LE++GCF   +DK+S+EL+ S+FEA ++LF L
Sbjct: 14  LPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFEL 73

Query: 73  PLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRAN 127
           P+ T+ +N S KP+HGY        LYES+ I++   L+         LWP   N
Sbjct: 74  PIPTKQRNVSSKPFHGYL----CHNLYESLGINDANVLEKVN-DFTQQLWPDHGN 123

[Close]
BLAST of Bhi09G000063 vs. TrEMBL
Match: tr|A0A1S3CDV2|A0A1S3CDV2_CUCME (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499759 PE=3 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.7e-52
Identity = 107/137 (78.10%), Postives = 121/137 (88.32%), Query Frame = 0 

Query: 7   EAKAK-VLPIIDLSKLEAVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEAT 66
           EAK+K V+P IDLSKLE VGT+EWG ACKEMR GLEEFGCFSVVYDKVSLELHNSI EAT
Sbjct: 4   EAKSKVVIPTIDLSKLE-VGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEAT 63

Query: 67  KELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRA 126
           KELFHLP+ETR KNTSEKPYHGYFGGYS LPLYESMAIDNP +L++TQ S ANL+WP+R 
Sbjct: 64  KELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQ-SFANLMWPTRD 123

Query: 127 NNHFCISFLVFKFNCLM 143
           NNHFC++  V +F+ L+
Sbjct: 124 NNHFCVT--VERFSTLV 136

[Close]
BLAST of Bhi09G000063 vs. TrEMBL
Match: tr|A0A0A0K8Y2|A0A0A0K8Y2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G434960 PE=4 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 3.0e-52
Identity = 106/137 (77.37%), Postives = 120/137 (87.59%), Query Frame = 0 

Query: 7   EAKAK-VLPIIDLSKLEAVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEAT 66
           EAK+K V+PIIDLSKLE  GT+EWG AC+EMR GLE+FGCFSVVYDKVSLELHNSIFEAT
Sbjct: 4   EAKSKVVIPIIDLSKLEE-GTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEAT 63

Query: 67  KELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRA 126
           KELFHLP+ETR KNTSEKPYHGYFGGYS LPLYESMAIDNP +LQ TQ S ANL+WP+  
Sbjct: 64  KELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQ-SFANLMWPTTE 123

Query: 127 NNHFCISFLVFKFNCLM 143
           NNHFC++  V +F+ L+
Sbjct: 124 NNHFCVA--VERFSTLV 136

[Close]
BLAST of Bhi09G000063 vs. TrEMBL
Match: tr|W9SCZ0|W9SCZ0_9ROSA (Gibberellin 3-beta-dioxygenase 4 OS=Morus notabilis OX=981085 GN=L484_019468 PE=3 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 2.4e-30
Identity = 62/125 (49.60%), Postives = 89/125 (71.20%), Query Frame = 0 

Query: 4   VNSEAKAKVLPIIDLSKLE-AVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIF 63
           + S++    +P+ID +K     GT+EW  ACKE+R G EE+GCF  VYDK+ ++LHNS+F
Sbjct: 1   MGSQSAEHKIPVIDFTKKNLKPGTDEWVLACKEVRNGFEEYGCFEAVYDKIPVDLHNSVF 60

Query: 64  EATKELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWP 123
            A + LF LPLET+ + TSE+PYH Y+G +S++PLYES+ +DNPT LQ ++    NL+WP
Sbjct: 61  AAAESLFDLPLETKMQKTSERPYHSYYGQFSVVPLYESLGVDNPTTLQGSR-KFTNLMWP 120

Query: 124 SRANN 128
           +   N
Sbjct: 121 AGNEN 124

[Close]
BLAST of Bhi09G000063 vs. TrEMBL
Match: tr|A5AE66|A5AE66_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_030994 PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.8e-28
Identity = 66/128 (51.56%), Postives = 87/128 (67.97%), Query Frame = 0 

Query: 4   VNSEAKAKVLPIIDLSKLEA-VGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIF 63
           + S + AK+ P++D SK     G+  W + CK++ L LEEFGCF  VYDKV  EL N +F
Sbjct: 1   MGSHSSAKI-PVVDFSKENLNPGSSSWSATCKDVLLALEEFGCFIAVYDKVPSELQNKLF 60

Query: 64  EATKELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWP 123
            A +ELF+LP ET+ +N   KPYHGY G +S LPL+ES+ IDN T L+  Q S ANL+WP
Sbjct: 61  SALEELFNLPTETKMQNAHNKPYHGYVGQHSXLPLHESIGIDNATTLEGXQ-SFANLMWP 120

Query: 124 SRANNHFC 131
           +  N+HFC
Sbjct: 121 A-GNDHFC 125

[Close]
BLAST of Bhi09G000063 vs. TrEMBL
Match: tr|M5WSX6|M5WSX6_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G076200 PE=3 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.3e-28
Identity = 65/130 (50.00%), Postives = 88/130 (67.69%), Query Frame = 0 

Query: 4   VNSEAKAKVLPIIDLSKLE-AVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIF 63
           + SE + K LP+IDLS      GTE W  A K ++  LEE+GCF  V+ KV L+LHNSIF
Sbjct: 1   MGSETQPK-LPVIDLSNENLKPGTEAWVLASKHVKYALEEYGCFEAVFHKVPLQLHNSIF 60

Query: 64  EATKELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWP 123
              ++LF LP+ET+ + TSE+PYH YFG YS LPLYES+ +DNPT ++  +     ++WP
Sbjct: 61  SVVEDLFDLPIETKRQQTSERPYHSYFGQYSFLPLYESLGVDNPTTVEGAK-GFTKIMWP 120

Query: 124 SRANNHFCIS 133
           +  N+HFC S
Sbjct: 121 A-GNDHFCES 127

[Close]
BLAST of Bhi09G000063 vs. NCBI nr
Match: XP_008461057.1 (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo])

HSP 1 Score: 214.5 bits (545), Expect = 2.6e-52
Identity = 107/137 (78.10%), Postives = 121/137 (88.32%), Query Frame = 0 

Query: 7   EAKAK-VLPIIDLSKLEAVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEAT 66
           EAK+K V+P IDLSKLE VGT+EWG ACKEMR GLEEFGCFSVVYDKVSLELHNSI EAT
Sbjct: 4   EAKSKVVIPTIDLSKLE-VGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEAT 63

Query: 67  KELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRA 126
           KELFHLP+ETR KNTSEKPYHGYFGGYS LPLYESMAIDNP +L++TQ S ANL+WP+R 
Sbjct: 64  KELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQ-SFANLMWPTRD 123

Query: 127 NNHFCISFLVFKFNCLM 143
           NNHFC++  V +F+ L+
Sbjct: 124 NNHFCVT--VERFSTLV 136

[Close]
BLAST of Bhi09G000063 vs. NCBI nr
Match: XP_004136073.1 (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus])

HSP 1 Score: 213.8 bits (543), Expect = 4.5e-52
Identity = 106/137 (77.37%), Postives = 120/137 (87.59%), Query Frame = 0 

Query: 7   EAKAK-VLPIIDLSKLEAVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEAT 66
           EAK+K V+PIIDLSKLE  GT+EWG AC+EMR GLE+FGCFSVVYDKVSLELHNSIFEAT
Sbjct: 4   EAKSKVVIPIIDLSKLEE-GTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEAT 63

Query: 67  KELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRA 126
           KELFHLP+ETR KNTSEKPYHGYFGGYS LPLYESMAIDNP +LQ TQ S ANL+WP+  
Sbjct: 64  KELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQ-SFANLMWPTTE 123

Query: 127 NNHFCISFLVFKFNCLM 143
           NNHFC++  V +F+ L+
Sbjct: 124 NNHFCVA--VERFSTLV 136

[Close]
BLAST of Bhi09G000063 vs. NCBI nr
Match: KGN45319.1 (hypothetical protein Csa_7G434960 [Cucumis sativus])

HSP 1 Score: 213.8 bits (543), Expect = 4.5e-52
Identity = 106/137 (77.37%), Postives = 120/137 (87.59%), Query Frame = 0 

Query: 7   EAKAK-VLPIIDLSKLEAVGTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSIFEAT 66
           EAK+K V+PIIDLSKLE  GT+EWG AC+EMR GLE+FGCFSVVYDKVSLELHNSIFEAT
Sbjct: 4   EAKSKVVIPIIDLSKLEE-GTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEAT 63

Query: 67  KELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLWPSRA 126
           KELFHLP+ETR KNTSEKPYHGYFGGYS LPLYESMAIDNP +LQ TQ S ANL+WP+  
Sbjct: 64  KELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQ-SFANLMWPTTE 123

Query: 127 NNHFCISFLVFKFNCLM 143
           NNHFC++  V +F+ L+
Sbjct: 124 NNHFCVA--VERFSTLV 136

[Close]
BLAST of Bhi09G000063 vs. NCBI nr
Match: XP_022992484.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima])

HSP 1 Score: 182.6 bits (462), Expect = 1.1e-42
Identity = 92/141 (65.25%), Postives = 110/141 (78.01%), Query Frame = 0 

Query: 4   VNSEAKAKVLPIIDLSKLEAV--GTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSI 63
           + SE +A+V P+IDLSKLE +  GTE WGS CK MR+GLEEFGCF VV+++VSLELHNS 
Sbjct: 1   MGSETQARV-PVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSF 60

Query: 64  FEATKELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLW 123
           F A  ELF LP+ETR KNTSEK YHGYFG YS LPLYESMAI+NPT+L   Q S ANL+W
Sbjct: 61  FGAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQ-SFANLMW 120

Query: 124 PSRANNHFCISFLVFKFNCLM 143
           P++ NNHF  S  + +F+ LM
Sbjct: 121 PAQGNNHF--SETIQRFSTLM 137

[Close]
BLAST of Bhi09G000063 vs. NCBI nr
Match: XP_022953943.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])

HSP 1 Score: 176.4 bits (446), Expect = 8.0e-41
Identity = 87/128 (67.97%), Postives = 100/128 (78.12%), Query Frame = 0 

Query: 4   VNSEAKAKVLPIIDLSKLEAV--GTEEWGSACKEMRLGLEEFGCFSVVYDKVSLELHNSI 63
           + SE +A V P+IDLSKLE +  GT+ WGS CK MR+GLEEFGCF VV+++VSLELHNS 
Sbjct: 1   MGSETQAGV-PVIDLSKLEKLEAGTDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSF 60

Query: 64  FEATKELFHLPLETRTKNTSEKPYHGYFGGYSLLPLYESMAIDNPTELQTTQLSLANLLW 123
           F A  ELF LP+ETR KNTSEK YHGYFG YS LPLYESMAIDNPT+L   Q S ANL+W
Sbjct: 61  FGAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQ-SFANLMW 120

Query: 124 PSRANNHF 130
           P + NN F
Sbjct: 121 PDQGNNRF 126

The following BLAST results are available for this feature:
BLAST of Bhi09G000063 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q945B6|AOP1L_ARATH3.2e-1738.26Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=... [more]
sp|Q9ZTA3|AOP1C_ARATH9.3e-1737.39Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37... [more]
sp|Q945B5|AOP2V_ARATH6.0e-1640.522-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP... [more]
sp|Q9ZTA2|AOP2C_ARATH1.0e-1540.52Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thali... [more]
sp|Q944Z5|AOP1V_ARATH1.3e-1536.52Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
BLAST of Bhi09G000063 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G52800.11.1e-2342.742-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT1G52810.16.5e-2139.062-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT1G52790.15.5e-2041.182-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT1G52820.17.2e-2040.542-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT4G03070.15.2e-1837.392-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
BLAST of Bhi09G000063 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3CDV2|A0A1S3CDV2_CUCME1.7e-5278.10probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX... [more]
tr|A0A0A0K8Y2|A0A0A0K8Y2_CUCSA3.0e-5277.37Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G434960 PE=4 SV=1[more]
tr|W9SCZ0|W9SCZ0_9ROSA2.4e-3049.60Gibberellin 3-beta-dioxygenase 4 OS=Morus notabilis OX=981085 GN=L484_019468 PE=... [more]
tr|A5AE66|A5AE66_VITVI1.8e-2851.56Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_030994 PE=3 SV=1[more]
tr|M5WSX6|M5WSX6_PRUPE2.3e-2850.00Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G076200 PE=3 SV=1[more]
BLAST of Bhi09G000063 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008461057.12.6e-5278.10PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumi... [more]
XP_004136073.14.5e-5277.37PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus][more]
KGN45319.14.5e-5277.37hypothetical protein Csa_7G434960 [Cucumis sativus][more]
XP_022992484.11.1e-4265.25probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima][more]
XP_022953943.18.0e-4167.97probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR027443IPNS-like
IPR026992DIOX_N
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0016491 oxidoreductase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi09M000063Bhi09M000063mRNA


InterPro
Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 13..90
e-value: 6.2E-13
score: 49.4
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 1..130
e-value: 2.8E-13
score: 51.8
NoneNo IPR availablePANTHERPTHR10209:SF1822-OXOGLUTARATE 2OG AND FE II -DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 10..124
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 10..124
NoneNo IPR availableSUPERFAMILYSSF51197Clavaminate synthase-likecoord: 12..124

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Bhi09G000063Csa7G434960Cucumber (Chinese Long) v2cuwgoB690
Bhi09G000063MELO3C023380Melon (DHL92) v3.5.1mewgoB239
Bhi09G000063Lsi02G027530Bottle gourd (USVL1VR-Ls)lsiwgoB270
Bhi09G000063Cla97C09G164600Watermelon (97103) v2wgowmbB614
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Bhi09G000063Cucumber (Gy14) v1cgywgoB051
Bhi09G000063Cucumber (Gy14) v1cgywgoB327
Bhi09G000063Cucumber (Gy14) v2cgybwgoB198
Bhi09G000063Cucumber (Gy14) v2cgybwgoB659
Bhi09G000063Cucurbita maxima (Rimu)cmawgoB0492
Bhi09G000063Cucurbita maxima (Rimu)cmawgoB0626
Bhi09G000063Cucurbita maxima (Rimu)cmawgoB1132
Bhi09G000063Cucurbita moschata (Rifu)cmowgoB0491
Bhi09G000063Cucurbita moschata (Rifu)cmowgoB0625
Bhi09G000063Cucurbita moschata (Rifu)cmowgoB1130
Bhi09G000063Cucurbita pepo (Zucchini)cpewgoB0208
Bhi09G000063Cucurbita pepo (Zucchini)cpewgoB0417
Bhi09G000063Cucurbita pepo (Zucchini)cpewgoB0721
Bhi09G000063Wild cucumber (PI 183967)cpiwgoB208
Bhi09G000063Wild cucumber (PI 183967)cpiwgoB702
Bhi09G000063Cucumber (Chinese Long) v3cucwgoB214
Bhi09G000063Cucumber (Chinese Long) v3cucwgoB715
Bhi09G000063Cucumber (Chinese Long) v2cuwgoB208
Bhi09G000063Bottle gourd (USVL1VR-Ls)lsiwgoB577
Bhi09G000063Melon (DHL92) v3.6.1medwgoB240
Bhi09G000063Melon (DHL92) v3.5.1mewgoB368
Bhi09G000063Watermelon (Charleston Gray)wcgwgoB068
Bhi09G000063Watermelon (Charleston Gray)wcgwgoB599
Bhi09G000063Watermelon (Charleston Gray)wcgwgoB659
Bhi09G000063Watermelon (97103) v2wgowmbB594
Bhi09G000063Watermelon (97103) v2wgowmbB607
Bhi09G000063Watermelon (97103) v1wgowmB585
Bhi09G000063Watermelon (97103) v1wgowmB604
Bhi09G000063Watermelon (97103) v1wgowmB614
Bhi09G000063Silver-seed gourdcarwgoB0150
Bhi09G000063Silver-seed gourdcarwgoB0184
Bhi09G000063Silver-seed gourdcarwgoB0253
Bhi09G000063Silver-seed gourdcarwgoB1204