Bhi08G000771 (gene) Wax gourd

NameBhi08G000771
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionEukaryotic aspartyl protease family protein
Locationchr8 : 29900864 .. 29902396 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCAATATCAACCTCCTTTGCAAAAAAAATCTTGAGCTATTTCCTTCTTCTTGTATATGTCTCAAGAAAAACCCTAGCTACAAACCCTAAAACCAATGGCCCCAAAGATTCTCTAGTTATTGGTCTTGTTCATTCAAGAACAACCCTCCTTACCCCAAAAAAAGGCTATAATTTCATTTCAAGGAAGAGAATGAAGGCAATGGAAATGGATGATAATGTGATAGAGCCATTGAGGGAAATTAGGGATGGTTATTTGATGTCCTTAACATTAGGGACACCCCCACAAGTTATTCAAGTCTATATGGACACAGGAAGTGATCTGACATGGGTTCCTTGTGGCAACCTCTCTTTTGATTGTCAAGATTGTGAGGAGTATCAAAACAATGTTTCAGGCCCAAAATTGGCTGCTTTTTTGCCTACTCATTCTTCTACTTCTATTAGAGACACTTGTGGTAGCTCCTTTTGTATGGATATTCATAGCTCTGATAACCCTTTTGATCCTTGCACAATTGCTGGTTGTTCCCTTGCTACCCTTGTGAAGGCCACTTGCCCTAGACCTTGCCCTTCATTTGCTTATACTTATGGAGCAAGTGGGGTTGTAATTGGAACTTTAACAAGAGATGTCCTTTTAATGCATATAAATAATATTAATTCTCCAAATTCTTCCACTAAGAAAACCCCTAGGTTTTGTTTTGGATGTGTTGGTGCAAGTTATAGAGAGCCAATTGGGATTGCTGGCTTTGGTAGAGGGTTACTTTCTCTTCCTTTTCAGTTAGGGTTTTCTCATAAGGGGTTTTCTCATTGCTTCTTGCCTTTTAAATTCTCAAATAACCCTAATTTTTCAAGCCCTTTGATTCTTGGTAACCTTGCTGTTTCTTCAAAAGATGAGCATTTGCAATTTACTCCTTTGTTGAAAAGTCCAATTTACCCCAACTACTACTATATTGGGCTTGAGTCAATCACTATAGGAAATGGGAATAGTAATTTTAGATTTGGAGTTTCTTTCAACTTGAGAGAGATTGATACAAAGGGTAATGGGGGAATGTTGATTGATTCTGGTACTACTTATACTCATTTACCTGAACCATTGTATTCACAACTTATTTCAAATCTTGAGTCAGTGATAAGCTATCCAAGAGCTAAACAAGTTGAACTCAATACTGGATTTGATCTTTGTTATAAAGTTCCTTGTAAAAACAACAATTTTTCTTTTATTGATGACTCTCAACTCCCTTCTATAACATTCCATTTTTTGAATAATGTTAGTGTTGTTTTGCCCCAAGAGAACAATTTCTATGCCATGGCTGCTCCAATTAACTCCACTGTGGTTAAATGCTTGTTGTTTCAAAGCATGGACGGTGTGGGTGGCGATACCGACGACGACCGAGATGGGCCGGCGGGTATTTTCGGAAGCTTCCAACAACAAAATTTGGAGGTTGTTTATGACTTGGAGAAGGAAAGATTAGGGTTTCAACCAATGGATTGTGCTTATGTTGCTGCCACTCAAGGACTACACAAGAATGTTTGA

mRNA sequence

ATGGCTTCAATATCAACCTCCTTTGCAAAAAAAATCTTGAGCTATTTCCTTCTTCTTGTATATGTCTCAAGAAAAACCCTAGCTACAAACCCTAAAACCAATGGCCCCAAAGATTCTCTAGTTATTGGTCTTGTTCATTCAAGAACAACCCTCCTTACCCCAAAAAAAGGCTATAATTTCATTTCAAGGAAGAGAATGAAGGCAATGGAAATGGATGATAATGTGATAGAGCCATTGAGGGAAATTAGGGATGGTTATTTGATGTCCTTAACATTAGGGACACCCCCACAAGTTATTCAAGTCTATATGGACACAGGAAGTGATCTGACATGGGTTCCTTGTGGCAACCTCTCTTTTGATTGTCAAGATTGTGAGGAGTATCAAAACAATGTTTCAGGCCCAAAATTGGCTGCTTTTTTGCCTACTCATTCTTCTACTTCTATTAGAGACACTTGTGGTAGCTCCTTTTGTATGGATATTCATAGCTCTGATAACCCTTTTGATCCTTGCACAATTGCTGGTTGTTCCCTTGCTACCCTTGTGAAGGCCACTTGCCCTAGACCTTGCCCTTCATTTGCTTATACTTATGGAGCAAGTGGGGTTGTAATTGGAACTTTAACAAGAGATGTCCTTTTAATGCATATAAATAATATTAATTCTCCAAATTCTTCCACTAAGAAAACCCCTAGGTTTTGTTTTGGATGTGTTGGTGCAAGTTATAGAGAGCCAATTGGGATTGCTGGCTTTGGTAGAGGGTTACTTTCTCTTCCTTTTCAGTTAGGGTTTTCTCATAAGGGGTTTTCTCATTGCTTCTTGCCTTTTAAATTCTCAAATAACCCTAATTTTTCAAGCCCTTTGATTCTTGGTAACCTTGCTGTTTCTTCAAAAGATGAGCATTTGCAATTTACTCCTTTGTTGAAAAGTCCAATTTACCCCAACTACTACTATATTGGGCTTGAGTCAATCACTATAGGAAATGGGAATAGTAATTTTAGATTTGGAGTTTCTTTCAACTTGAGAGAGATTGATACAAAGGGTAATGGGGGAATGTTGATTGATTCTGGTACTACTTATACTCATTTACCTGAACCATTGTATTCACAACTTATTTCAAATCTTGAGTCAGTGATAAGCTATCCAAGAGCTAAACAAGTTGAACTCAATACTGGATTTGATCTTTGTTATAAAGTTCCTTGTAAAAACAACAATTTTTCTTTTATTGATGACTCTCAACTCCCTTCTATAACATTCCATTTTTTGAATAATGTTAGTGTTGTTTTGCCCCAAGAGAACAATTTCTATGCCATGGCTGCTCCAATTAACTCCACTGTGGTTAAATGCTTGTTGTTTCAAAGCATGGACGGTGTGGGTGGCGATACCGACGACGACCGAGATGGGCCGGCGGGTATTTTCGGAAGCTTCCAACAACAAAATTTGGAGGTTGTTTATGACTTGGAGAAGGAAAGATTAGGGTTTCAACCAATGGATTGTGCTTATGTTGCTGCCACTCAAGGACTACACAAGAATGTTTGA

Coding sequence (CDS)

ATGGCTTCAATATCAACCTCCTTTGCAAAAAAAATCTTGAGCTATTTCCTTCTTCTTGTATATGTCTCAAGAAAAACCCTAGCTACAAACCCTAAAACCAATGGCCCCAAAGATTCTCTAGTTATTGGTCTTGTTCATTCAAGAACAACCCTCCTTACCCCAAAAAAAGGCTATAATTTCATTTCAAGGAAGAGAATGAAGGCAATGGAAATGGATGATAATGTGATAGAGCCATTGAGGGAAATTAGGGATGGTTATTTGATGTCCTTAACATTAGGGACACCCCCACAAGTTATTCAAGTCTATATGGACACAGGAAGTGATCTGACATGGGTTCCTTGTGGCAACCTCTCTTTTGATTGTCAAGATTGTGAGGAGTATCAAAACAATGTTTCAGGCCCAAAATTGGCTGCTTTTTTGCCTACTCATTCTTCTACTTCTATTAGAGACACTTGTGGTAGCTCCTTTTGTATGGATATTCATAGCTCTGATAACCCTTTTGATCCTTGCACAATTGCTGGTTGTTCCCTTGCTACCCTTGTGAAGGCCACTTGCCCTAGACCTTGCCCTTCATTTGCTTATACTTATGGAGCAAGTGGGGTTGTAATTGGAACTTTAACAAGAGATGTCCTTTTAATGCATATAAATAATATTAATTCTCCAAATTCTTCCACTAAGAAAACCCCTAGGTTTTGTTTTGGATGTGTTGGTGCAAGTTATAGAGAGCCAATTGGGATTGCTGGCTTTGGTAGAGGGTTACTTTCTCTTCCTTTTCAGTTAGGGTTTTCTCATAAGGGGTTTTCTCATTGCTTCTTGCCTTTTAAATTCTCAAATAACCCTAATTTTTCAAGCCCTTTGATTCTTGGTAACCTTGCTGTTTCTTCAAAAGATGAGCATTTGCAATTTACTCCTTTGTTGAAAAGTCCAATTTACCCCAACTACTACTATATTGGGCTTGAGTCAATCACTATAGGAAATGGGAATAGTAATTTTAGATTTGGAGTTTCTTTCAACTTGAGAGAGATTGATACAAAGGGTAATGGGGGAATGTTGATTGATTCTGGTACTACTTATACTCATTTACCTGAACCATTGTATTCACAACTTATTTCAAATCTTGAGTCAGTGATAAGCTATCCAAGAGCTAAACAAGTTGAACTCAATACTGGATTTGATCTTTGTTATAAAGTTCCTTGTAAAAACAACAATTTTTCTTTTATTGATGACTCTCAACTCCCTTCTATAACATTCCATTTTTTGAATAATGTTAGTGTTGTTTTGCCCCAAGAGAACAATTTCTATGCCATGGCTGCTCCAATTAACTCCACTGTGGTTAAATGCTTGTTGTTTCAAAGCATGGACGGTGTGGGTGGCGATACCGACGACGACCGAGATGGGCCGGCGGGTATTTTCGGAAGCTTCCAACAACAAAATTTGGAGGTTGTTTATGACTTGGAGAAGGAAAGATTAGGGTTTCAACCAATGGATTGTGCTTATGTTGCTGCCACTCAAGGACTACACAAGAATGTTTGA

Protein sequence

MASISTSFAKKILSYFLLLVYVSRKTLATNPKTNGPKDSLVIGLVHSRTTLLTPKKGYNFISRKRMKAMEMDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCVGASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSKDEHLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITFHFLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQNLEVVYDLEKERLGFQPMDCAYVAATQGLHKNV
BLAST of Bhi08G000771 vs. Swiss-Prot
Match: sp|Q940R4|ASP63_ARATH (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 5.4e-47
Identity = 141/447 (31.54%), Postives = 201/447 (44.97%), Query Frame = 0

Query: 86  YLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVSGPKLAAFLPTHSS 145
           YL+SL++G+    + +Y+DTGSDL W PC    F C  CE      S P   +       
Sbjct: 83  YLISLSVGSSSSAVSLYLDTGSDLVWFPC--RPFTCILCE------SKPLPPSXXXXXXX 142

Query: 146 TSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKATC---PRPCPSFAYTYGASGVV 205
                                 D C I+ C L  +    C     PCP F Y YG   + 
Sbjct: 143 XXXXXXXXXXXXXXXXXXLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGSL- 202

Query: 206 IGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCVGASYREPIGIAGFGRGLLSLPFQLGF 265
                  V  ++ ++++ P+ S      F FGC   +  EPIG+AGFGRG LSLP QL  
Sbjct: 203 -------VAKLYSDSLSLPSVSVS---NFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAV 262

Query: 266 --SHKG--FSHCFLPFKF-SNNPNFSSPLILGNLAVSSKDEHL----------------- 325
              H G  FS+C +   F S+     SPLILG   V  K++ +                 
Sbjct: 263 HSPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRF-VDKKEKRVGXXXXXXXXXXXXXXXX 322

Query: 326 --QFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTY 385
              FT +L++P +P +Y + L+ I+IG  N          LR ID  G GG+++DSGTT+
Sbjct: 323 EFVFTEMLENPKHPYFYSVSLQGISIGKRN----IPAPAMLRRIDKNGGGGVVVDSGTTF 382

Query: 386 THLPEPLYSQLISNLESVIS--YPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSIT 445
           T LP   Y+ ++   +S +   + RA +VE ++G   CY +             ++P++ 
Sbjct: 383 TMLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN---------QTVKVPALV 442

Query: 446 FHFL-NNVSVVLPQENNFYAMA----APINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIF 499
            HF  N  SV LP+ N FY              + CL+  +    GGD  + R G   I 
Sbjct: 443 LHFAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMN----GGDESELRGGTGAIL 492

BLAST of Bhi08G000771 vs. Swiss-Prot
Match: sp|Q766C2|NEP2_NEPGR (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 6.8e-34
Identity = 125/439 (28.47%), Postives = 177/439 (40.32%), Query Frame = 0

Query: 64  KRMKAMEMDDNVIEPLREIRDG-YLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQ 123
           + + AM    + IE      DG YLM++ +GTP       MDTGSDL W  C      C 
Sbjct: 73  RSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCE----PCT 132

Query: 124 DCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVK 183
            C      +       F P  SS+     C S +C D+     P + C    C       
Sbjct: 133 QCFSQPTPI-------FNPQDSSSFSTLPCESQYCQDL-----PSETCNNNEC------- 192

Query: 184 ATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGC----VGA 243
                    + Y YG      G +  +               T   P   FGC     G 
Sbjct: 193 --------QYTYGYGDGSTTQGYMATETFTF----------ETSSVPNIAFGCGEDNQGF 252

Query: 244 SYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSKDE 303
                 G+ G G G LSLP QLG     FS+C   +  S+     S L LG+ A S   E
Sbjct: 253 GQGNGAGLIGMGWGPLSLPSQLGVGQ--FSYCMTSYGSSS----PSTLALGS-AASGVPE 312

Query: 304 HLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTY 363
               T L+ S + P YYYI L+ IT+G  N     G+  +  ++   G GGM+IDSGTT 
Sbjct: 313 GSPSTTLIHSSLNPTYYYITLQGITVGGDN----LGIPSSTFQLQDDGTGGMIIDSGTTL 372

Query: 364 THLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITFH 423
           T+LP+  Y+ +       I+ P     E ++G   C++ P   +        Q+P I+  
Sbjct: 373 TYLPQDAYNAVAQAFTDQINLPTVD--ESSSGLSTCFQQPSDGSTV------QVPEISMQ 432

Query: 424 FLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQN 483
           F   V + L ++N    + +P    +  CL   S   +G            IFG+ QQQ 
Sbjct: 433 FDGGV-LNLGEQN---ILISPAEGVI--CLAMGSSSQLG----------ISIFGNIQQQE 435

Query: 484 LEVVYDLEKERLGFQPMDC 498
            +V+YDL+   + F P  C
Sbjct: 493 TQVLYDLQNLAVSFVPTQC 435

BLAST of Bhi08G000771 vs. Swiss-Prot
Match: sp|Q9LNJ3|APF2_ARATH (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 7.5e-33
Identity = 124/428 (28.97%), Postives = 185/428 (43.22%), Query Frame = 0

Query: 74  NVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVSG 133
           +V+  L +    Y   L +GTP + + + +DTGSD+ W+ C      C+ C    + +  
Sbjct: 130 SVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCA----PCRRCYSQSDPI-- 189

Query: 134 PKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKATCPRPCPSFA 193
                F P  S T     C S  C  + S          AGC+     + TC      + 
Sbjct: 190 -----FDPRKSKTYATIPCSSPHCRRLDS----------AGCNTR---RKTC-----LYQ 249

Query: 194 YTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCVGASYREPIGIAGFGRGL 253
            +YG     +G  + + L    N +              F  VGA+     G+ G G+G 
Sbjct: 250 VSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLF--VGAA-----GLLGLGKGK 309

Query: 254 LSLPFQLG--FSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSKDEHLQFTPLLKSPIY 313
           LS P Q G  F+ K FS+C +    S+ P   S ++ GN AVS      +FTPLL +P  
Sbjct: 310 LSFPGQTGHRFNQK-FSYCLVDRSASSKP---SSVVFGNAAVS---RIARFTPLLSNPKL 369

Query: 314 PNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLIS 373
             +YY+GL  I++G        GV+ +L ++D  GNGG++IDSGT+ T L  P Y  +  
Sbjct: 370 DTFYYVGLLGISVGGTRVP---GVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRD 429

Query: 374 NLE-SVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITFHFLNNVSVVLPQE 433
                  +  RA    L   FD C+       + S +++ ++P++  HF     V LP  
Sbjct: 430 AFRVGAKTLKRAPDFSL---FDTCF-------DLSNMNEVKVPTVVLHF-RGADVSLPAT 485

Query: 434 NNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQNLEVVYDLEKERL 493
           N       P+++    C  F     +GG           I G+ QQQ   VVYDL   R+
Sbjct: 490 N----YLIPVDTNGKFCFAFAGT--MGG---------LSIIGNIQQQGFRVVYDLASSRV 485

Query: 494 GFQPMDCA 499
           GF P  CA
Sbjct: 550 GFAPGGCA 485

BLAST of Bhi08G000771 vs. Swiss-Prot
Match: sp|Q766C3|NEP1_NEPGR (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 4.1e-31
Identity = 129/443 (29.12%), Postives = 181/443 (40.86%), Query Frame = 0

Query: 64  KRMKAMEMDDNVIEPLREIRDG-YLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQ 123
           +R++AM    + +E      DG YLM+L++GTP Q     MDTGSDL W         CQ
Sbjct: 72  QRLEAMLNGPSGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIWT-------QCQ 131

Query: 124 DCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVK 183
            C +  N         F P  SS+     C S  C  + S                    
Sbjct: 132 PCTQCFNQ----STPIFNPQGSSSFSTLPCSSQLCQALSS-------------------- 191

Query: 184 ATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGC----VGA 243
            TC      + Y YG      G++  + L             +   P   FGC     G 
Sbjct: 192 PTCSNNFCQYTYGYGDGSETQGSMGTETLTF----------GSVSIPNITFGCGENNQGF 251

Query: 244 SYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSKDE 303
                 G+ G GRG LSLP QL  +   FS+C  P   S   N    L+LG+LA +S   
Sbjct: 252 GQGNGAGLVGMGRGPLSLPSQLDVTK--FSYCMTPIGSSTPSN----LLLGSLA-NSVTA 311

Query: 304 HLQFTPLLKSPIYPNYYYIGLESITIGNGN---SNFRFGVSFNLREIDTKGNGGMLIDSG 363
               T L++S   P +YYI L  +++G+         F ++ N       G GG++IDSG
Sbjct: 312 GSPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSN------NGTGGIIIDSG 371

Query: 364 TTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSI 423
           TT T+     Y  +     S I+ P       ++GFDLC++ P   +N       Q+P+ 
Sbjct: 372 TTLTYFVNNAYQSVRQEFISQINLPVVN--GSSSGFDLCFQTPSDPSNL------QIPTF 431

Query: 424 TFHFLNNVSVVLPQENNFYAMAAPINSTVVKCL-LFQSMDGVGGDTDDDRDGPAGIFGSF 483
             HF +   + LP EN F    +P N  +  CL +  S  G+             IFG+ 
Sbjct: 432 VMHF-DGGDLELPSENYF---ISPSNGLI--CLAMGSSSQGM------------SIFGNI 434

Query: 484 QQQNLEVVYDLEKERLGFQPMDC 498
           QQQN+ VVYD     + F    C
Sbjct: 492 QQQNMLVVYDTGNSVVSFASAQC 434

BLAST of Bhi08G000771 vs. Swiss-Prot
Match: sp|Q6XBF8|CDR1_ARATH (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 1.5e-28
Identity = 124/435 (28.51%), Postives = 183/435 (42.07%), Query Frame = 0

Query: 73  DNVIEPLREIRDG---YLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQN 132
           DN  +P  ++      YLM++++GTPP  I    DTGSDL W  C      C DC    +
Sbjct: 74  DNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCA----PCDDCYTQVD 133

Query: 133 NVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKATCPRPC 192
            +  PK        SST    +C SS C  + +          A CS       T    C
Sbjct: 134 PLFDPKT-------SSTYKDVSCSSSQCTALENQ---------ASCS-------TNDNTC 193

Query: 193 PSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGC----VGASYREPIG 252
            S++ +YG +    G +  D L      + S ++   +      GC     G   ++  G
Sbjct: 194 -SYSLSYGDNSYTKGNIAVDTL-----TLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSG 253

Query: 253 IAGFGRGLLSLPFQLGFSHKG-FSHCFLPFKFSNNPNFSSPLILGNLAVSSKDEHLQFTP 312
           I G G G +SL  QLG S  G FS+C +P   ++  + +S +  G  A+ S    +  TP
Sbjct: 254 IVGLGGGPVSLIKQLGDSIDGKFSYCLVP--LTSKKDQTSKINFGTNAIVS-GSGVVSTP 313

Query: 313 LLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTYTHLPEP 372
           L+       +YY+ L+SI++G+    +    S           G ++IDSGTT T LP  
Sbjct: 314 LIAKASQETFYYLTLKSISVGSKQIQYSGSDS-------ESSEGNIIIDSGTTLTLLPTE 373

Query: 373 LYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITFHFLNNVS 432
            YS+L   + S  S    K+ +  +G  LCY         S   D ++P IT HF +   
Sbjct: 374 FYSELEDAVAS--SIDAEKKQDPQSGLSLCY---------SATGDLKVPVITMHF-DGAD 433

Query: 433 VVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPA-GIFGSFQQQNLEVVY 492
           V L   N F  +     S  + C  F             R  P+  I+G+  Q N  V Y
Sbjct: 434 VKLDSSNAFVQV-----SEDLVCFAF-------------RGSPSFSIYGNVAQMNFLVGY 435

Query: 493 DLEKERLGFQPMDCA 499
           D   + + F+P DCA
Sbjct: 494 DTVSKTVSFKPTDCA 435

BLAST of Bhi08G000771 vs. TAIR10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein)

HSP 1 Score: 577.8 bits (1488), Expect = 6.6e-165
Identity = 279/447 (62.42%), Postives = 346/447 (77.40%), Query Frame = 0

Query: 67  KAMEMDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEE 126
           K +   D V+EPLRE+RDGYL++L +GTPPQ +QVY+DTGSDLTWVPCGNLSFDC +C +
Sbjct: 64  KPLSSVDVVMEPLREVRDGYLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSFDCIECYD 123

Query: 127 YQNN-VSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKATC 186
            +NN +  P  + F P HSSTS RD+C SSFC++IHSSDNPFDPC +AGCS++ L+K+TC
Sbjct: 124 LKNNDLKSP--SVFSPLHSSTSFRDSCASSFCVEIHSSDNPFDPCAVAGCSVSMLLKSTC 183

Query: 187 PRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCVGASYREPIG 246
            RPCPSFAYTYG  G++ G LTRD+L           + T+  PRF FGCV ++YREPIG
Sbjct: 184 VRPCPSFAYTYGEGGLISGILTRDIL----------KARTRDVPRFSFGCVTSTYREPIG 243

Query: 247 IAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVS-SKDEHLQFTP 306
           IAGFGRGLLSLP QLGF  KGFSHCFLPFKF NNPN SSPLILG  A+S +  + LQFTP
Sbjct: 244 IAGFGRGLLSLPSQLGFLEKGFSHCFLPFKFVNNPNISSPLILGASALSINLTDSLQFTP 303

Query: 307 LLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTYTHLPEP 366
           +L +P+YPN YYIGLESITIG   +  +  V   LR+ D++GNGGML+DSGTTYTHLPEP
Sbjct: 304 MLNTPMYPNSYYIGLESITIGTNITPTQ--VPLTLRQFDSQGNGGMLVDSGTTYTHLPEP 363

Query: 367 LYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQL---PSITFHFLN 426
            YSQL++ L+S I+YPRA + E  TGFDLCYKVPC NNN + +++  +   PSITFHFLN
Sbjct: 364 FYSQLLTTLQSTITYPRATETESRTGFDLCYKVPCPNNNLTSLENDVMMIFPSITFHFLN 423

Query: 427 NVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQNLEV 486
           N +++LPQ N+FYAM+AP + +VV+CLLFQ+M       +D   GPAG+FGSFQQQN++V
Sbjct: 424 NATLLLPQGNSFYAMSAPSDGSVVQCLLFQNM-------EDGDYGPAGVFGSFQQQNVKV 483

Query: 487 VYDLEKERLGFQPMDCAYVAATQGLHK 509
           VYDLEKER+GFQ MDC   AA+ GL++
Sbjct: 484 VYDLEKERIGFQAMDCVLEAASHGLNQ 489

BLAST of Bhi08G000771 vs. TAIR10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein)

HSP 1 Score: 190.3 bits (482), Expect = 3.0e-48
Identity = 141/447 (31.54%), Postives = 201/447 (44.97%), Query Frame = 0

Query: 86  YLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVSGPKLAAFLPTHSS 145
           YL+SL++G+    + +Y+DTGSDL W PC    F C  CE      S P   +       
Sbjct: 83  YLISLSVGSSSSAVSLYLDTGSDLVWFPC--RPFTCILCE------SKPLPPSXXXXXXX 142

Query: 146 TSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKATC---PRPCPSFAYTYGASGVV 205
                                 D C I+ C L  +    C     PCP F Y YG   + 
Sbjct: 143 XXXXXXXXXXXXXXXXXXLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYGDGSL- 202

Query: 206 IGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCVGASYREPIGIAGFGRGLLSLPFQLGF 265
                  V  ++ ++++ P+ S      F FGC   +  EPIG+AGFGRG LSLP QL  
Sbjct: 203 -------VAKLYSDSLSLPSVSVS---NFTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAV 262

Query: 266 --SHKG--FSHCFLPFKF-SNNPNFSSPLILGNLAVSSKDEHL----------------- 325
              H G  FS+C +   F S+     SPLILG   V  K++ +                 
Sbjct: 263 HSPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRF-VDKKEKRVGXXXXXXXXXXXXXXXX 322

Query: 326 --QFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTY 385
              FT +L++P +P +Y + L+ I+IG  N          LR ID  G GG+++DSGTT+
Sbjct: 323 EFVFTEMLENPKHPYFYSVSLQGISIGKRN----IPAPAMLRRIDKNGGGGVVVDSGTTF 382

Query: 386 THLPEPLYSQLISNLESVIS--YPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSIT 445
           T LP   Y+ ++   +S +   + RA +VE ++G   CY +             ++P++ 
Sbjct: 383 TMLPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN---------QTVKVPALV 442

Query: 446 FHFL-NNVSVVLPQENNFYAMA----APINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIF 499
            HF  N  SV LP+ N FY              + CL+  +    GGD  + R G   I 
Sbjct: 443 LHFAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMN----GGDESELRGGTGAIL 492

BLAST of Bhi08G000771 vs. TAIR10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein)

HSP 1 Score: 174.9 bits (442), Expect = 1.3e-43
Identity = 132/423 (31.21%), Postives = 198/423 (46.81%), Query Frame = 0

Query: 85  GYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVSGPKLAAFLPTHS 144
           GY +SL+ GTP Q I    DTGS L W+PC +  + C  C+   + +    +  F+P +S
Sbjct: 89  GYSVSLSFGTPSQTIPFVFDTGSSLVWLPCTS-RYLCSGCD--FSGLDPTLIPRFIPKNS 148

Query: 145 STSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKATCPRPCPSFAYTYGASGVVIG 204
           S+S    C S  C  ++    P   C   GC   T     C   CP +   YG  G   G
Sbjct: 149 SSSKIIGCQSPKCQFLY---GPNVQC--RGCDPNT---RNCTVGCPPYILQYGL-GSTAG 208

Query: 205 TLTRDVLLMHINNINSPNSSTKKTPRFCFGCVGASYREPIGIAGFGRGLLSLPFQLGFSH 264
            L  +        ++ P+ +    P F  GC   S R+P GIAGFGRG +SLP Q+    
Sbjct: 209 VLITE-------KLDFPDLT---VPDFVVGCSIISTRQPAGIAGFGRGPVSLPSQMNL-- 268

Query: 265 KGFSHCFLPFKFSNNPNFSSPLILGNLA---VSSKDEHLQFTPLLKSPIYPN-----YYY 324
           K FSHC +  +F ++ N ++ L L   +     SK   L +TP  K+P   N     YYY
Sbjct: 269 KRFSHCLVSRRF-DDTNVTTDLDLDTGSGHNSGSKTPGLTYTPFRKNPNVSNKAFLEYYY 328

Query: 325 IGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLESV 384
           + L  I +G  +      + +      T G+GG ++DSG+T+T +  P++  +     S 
Sbjct: 329 LNLRRIYVGRKHVK----IPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAEEFASQ 388

Query: 385 IS-YPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITFHFLNNVSVVLPQENNFYA 444
           +S Y R K +E  TG   C+ +  K        D  +P + F F     + LP  +N++ 
Sbjct: 389 MSNYTREKDLEKETGLGPCFNISGKG-------DVTVPELIFEFKGGAKLELPL-SNYFT 448

Query: 445 MAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQNLEVVYDLEKERLGFQPM 499
                ++  +  +  ++++  GG       GPA I GSFQQQN  V YDLE +R GF   
Sbjct: 449 FVGNTDTVCLTVVSDKTVNPSGG------TGPAIILGSFQQQNYLVEYDLENDRFGFAKK 468

BLAST of Bhi08G000771 vs. TAIR10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein)

HSP 1 Score: 159.8 bits (403), Expect = 4.3e-39
Identity = 128/451 (28.38%), Postives = 187/451 (41.46%), Query Frame = 0

Query: 59  NFISRKRMKAMEMDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLS 118
           +F+S +R     +   V+         Y + L +G PPQ + +  DTGSDL WV C    
Sbjct: 57  HFLSLRRKPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCS--- 116

Query: 119 FDCQDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLA 178
             C++C  +           F P HSST     C    C  +   D        A     
Sbjct: 117 -ACRNCSHHS------PATVFFPRHSSTFSPAHCYDPVCRLVPKPDR-------APICNH 176

Query: 179 TLVKATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGC--- 238
           T + +TC        Y YG +    G+LT  +      ++ + +    +     FGC   
Sbjct: 177 TRIHSTC-------HYEYGYAD---GSLTSGLFARETTSLKTSSGKEARLKSVAFGCGFR 236

Query: 239 ------VGASYREPIGIAGFGRGLLSLPFQLG--FSHKGFSHCFLPFKFSNNPNFSSPLI 298
                  G S+    G+ G GRG +S   QLG  F +K FS+C + +  S  P  +S LI
Sbjct: 237 ISGQSVSGTSFNGANGVMGLGRGPISFASQLGRRFGNK-FSYCLMDYTLSPPP--TSYLI 296

Query: 299 LGNLAVSSKDEHLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGN 358
           +GN         L FTPLL +P+ P +YY+ L+S+ +       +  +  ++ EID  GN
Sbjct: 297 IGN--GGDGISKLFFTPLLTNPLSPTFYYVKLKSVFVNGA----KLRIDPSIWEIDDSGN 356

Query: 359 GGMLIDSGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFI 418
           GG ++DSGTT   L EP Y  +I+ +   +  P A    L  GFDLC  V          
Sbjct: 357 GGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIAD--ALTPGFDLCVNVSGVTK----- 416

Query: 419 DDSQLPSITFHFLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGP 478
            +  LP + F F      V P  N F           ++CL  QS+D   G         
Sbjct: 417 PEKILPRLKFEFSGGAVFVPPPRNYFIE-----TEEQIQCLAIQSVDPKVG--------- 450

Query: 479 AGIFGSFQQQNLEVVYDLEKERLGFQPMDCA 499
             + G+  QQ     +D ++ RLGF    CA
Sbjct: 477 FSVIGNLMQQGFLFEFDRDRSRLGFSRRGCA 450

BLAST of Bhi08G000771 vs. TAIR10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein)

HSP 1 Score: 143.3 bits (360), Expect = 4.2e-34
Identity = 124/428 (28.97%), Postives = 185/428 (43.22%), Query Frame = 0

Query: 74  NVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVSG 133
           +V+  L +    Y   L +GTP + + + +DTGSD+ W+ C      C+ C    + +  
Sbjct: 130 SVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCA----PCRRCYSQSDPI-- 189

Query: 134 PKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKATCPRPCPSFA 193
                F P  S T     C S  C  + S          AGC+     + TC      + 
Sbjct: 190 -----FDPRKSKTYATIPCSSPHCRRLDS----------AGCNTR---RKTC-----LYQ 249

Query: 194 YTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCVGASYREPIGIAGFGRGL 253
            +YG     +G  + + L    N +              F  VGA+     G+ G G+G 
Sbjct: 250 VSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLF--VGAA-----GLLGLGKGK 309

Query: 254 LSLPFQLG--FSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSKDEHLQFTPLLKSPIY 313
           LS P Q G  F+ K FS+C +    S+ P   S ++ GN AVS      +FTPLL +P  
Sbjct: 310 LSFPGQTGHRFNQK-FSYCLVDRSASSKP---SSVVFGNAAVS---RIARFTPLLSNPKL 369

Query: 314 PNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLIS 373
             +YY+GL  I++G        GV+ +L ++D  GNGG++IDSGT+ T L  P Y  +  
Sbjct: 370 DTFYYVGLLGISVGGTRVP---GVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRD 429

Query: 374 NLE-SVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITFHFLNNVSVVLPQE 433
                  +  RA    L   FD C+       + S +++ ++P++  HF     V LP  
Sbjct: 430 AFRVGAKTLKRAPDFSL---FDTCF-------DLSNMNEVKVPTVVLHF-RGADVSLPAT 485

Query: 434 NNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQNLEVVYDLEKERL 493
           N       P+++    C  F     +GG           I G+ QQQ   VVYDL   R+
Sbjct: 490 N----YLIPVDTNGKFCFAFAGT--MGG---------LSIIGNIQQQGFRVVYDLASSRV 485

Query: 494 GFQPMDCA 499
           GF P  CA
Sbjct: 550 GFAPGGCA 485

BLAST of Bhi08G000771 vs. TrEMBL
Match: tr|A0A1S3CAK9|A0A1S3CAK9_CUCME (aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103498305 PE=3 SV=1)

HSP 1 Score: 923.7 bits (2386), Expect = 1.8e-265
Identity = 450/512 (87.89%), Postives = 471/512 (91.99%), Query Frame = 0

Query: 5   STSFAKKILSYFLLLVYVSRKTLATNPKTNGPKDSLVIGLVHSRTTLLTPKKGYNFISRK 64
           STS A K LS FLLLV+ S++TLATNPKTN PKDSLV+GLVHSRT+LLTPKKGYNFIS+K
Sbjct: 6   STSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYNFISKK 65

Query: 65  RMKAMEM---DDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDC 124
           RMKAM+    DDNVIEPLREIRDGYLMSL++GTPPQV+QVYMDTGSDLTWVPCGNLSFDC
Sbjct: 66  RMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDC 125

Query: 125 QDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLV 184
           QDCEEYQNN+SGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLV
Sbjct: 126 QDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLV 185

Query: 185 KATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHIN---NINSPNSSTKKTPRFCFGCVGA 244
           K TCPRPCPSFAYTYGASGVV G+LTRDVL MH N               PRFCFGCVGA
Sbjct: 186 KGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYXXXXXXXXXXXXXVPRFCFGCVGA 245

Query: 245 SYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSKDE 304
           +YREPIGIAGFGRGLLSLPFQLGFS KGFSHCFLPFKFSNNPNFSSPLILG+LA+SSKDE
Sbjct: 246 TYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAISSKDE 305

Query: 305 HLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTY 364
           +LQFTPLLKSPIYPNYYYIGLESITIGNGN+NFRFGVSF LREIDTKGNGGMLIDSGTTY
Sbjct: 306 NLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTY 365

Query: 365 THLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITFH 424
           THLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNN SF+DDSQLPSITFH
Sbjct: 366 THLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPSITFH 425

Query: 425 FLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQN 484
           FLNNVSVVLPQ NNFYAMAAPINSTVVKCLL+QSMDGVG D D D +GPAGIFGSFQQQN
Sbjct: 426 FLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQN 485

Query: 485 LEVVYDLEKERLGFQPMDCAYVAATQGLHKNV 511
           L+VVYDLEKERLGFQ MDC  VAA QGLHKNV
Sbjct: 486 LQVVYDLEKERLGFQAMDCVSVAANQGLHKNV 517

BLAST of Bhi08G000771 vs. TrEMBL
Match: tr|A0A0A0LYP0|A0A0A0LYP0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G704590 PE=3 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 3.4e-264
Identity = 448/514 (87.16%), Postives = 474/514 (92.22%), Query Frame = 0

Query: 1   MASIST-SFAKKILSYFLLLVYVSRKTLATNPKTNGPKDSLVIGLVHSRTTLLTPKKGYN 60
           M SIS+ S A K LS FLLLV+VS +TLATNPKTN PKDSLV+GLVHSRT+LLTPKKGYN
Sbjct: 1   MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  FISRKRMKAMEM---DDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120
           FIS+KRMKAM+    DDNVIEPLREIRDGYLMSL++GTPPQV+QVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNN+SGP+LAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCV 240
           LA+LVK TCPRPCPSFAYTYGASGVV G+LTRDVL  H  N         + PRFCFGCV
Sbjct: 181 LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTH-GNYXXXXXXXXQIPRFCFGCV 240

Query: 241 GASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSK 300
           GA+YREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLA+SSK
Sbjct: 241 GATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK 300

Query: 301 DEHLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGT 360
           DE+LQFTPLLKSP+YPNYYYIGLESITIGNG++NFRFGVSF LREIDTKGNGGMLIDSGT
Sbjct: 301 DENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGT 360

Query: 361 TYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSIT 420
           TYTHLPEPLYSQLISNLE VI YPRAKQVELNTGFDLCYKVPCKNNN SF+DD+QLPSIT
Sbjct: 361 TYTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSIT 420

Query: 421 FHFLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQ 480
           FHFLNNVSVVLPQ NNFYAMAAPINSTVVKCLL+QSMDGVG D D D +GPAGIFGSFQQ
Sbjct: 421 FHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQ 480

Query: 481 QNLEVVYDLEKERLGFQPMDCAYVAATQGLHKNV 511
           QN+EVVYDLEKERLGFQPMDC  VAA QGLHKNV
Sbjct: 481 QNIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNV 513

BLAST of Bhi08G000771 vs. TrEMBL
Match: tr|A0A2R6R206|A0A2R6R206_ACTCH (Aspartyl protease OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc11327 PE=3 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 1.1e-182
Identity = 320/475 (67.37%), Postives = 386/475 (81.26%), Query Frame = 0

Query: 39  SLVIGLVHSRTTLLTPKKGYNFISRKRMKAMEMDDNVIEPLREIRDGYLMSLTLGTPPQV 98
           SLV+GL HSR  L TP +  +F S    K  EM D ++EPLRE+RDGY++SLTLGTPPQV
Sbjct: 34  SLVLGLKHSRAILPTPMEA-SFDSTSNYKPAEMLD-MMEPLREVRDGYIISLTLGTPPQV 93

Query: 99  IQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCM 158
           I VYMDTGSDLTWVPCGNLSFDC DC++++NN     +A+F P++SS+S RD+C S FC+
Sbjct: 94  IPVYMDTGSDLTWVPCGNLSFDCMDCDDHRNN---KLMASFSPSYSSSSFRDSCASPFCI 153

Query: 159 DIHSSDNPFDPCTIAGCSLATLVKATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNI 218
           D+HSSDN +DPCT+AGCSL+TLVKATC RPCPSFAYTYG  GVV+G+LTRD+L +H   I
Sbjct: 154 DVHSSDNSYDPCTVAGCSLSTLVKATCSRPCPSFAYTYG-DGVVVGSLTRDILRVH--GI 213

Query: 219 NSPNSSTKKTPRFCFGCVGASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSN 278
           +SP   T++  +FCFGCVG++YREPIGIAGFG+G LSLP QLG+  KGFSHCF+ FKF+N
Sbjct: 214 SSP-GVTREISKFCFGCVGSTYREPIGIAGFGKGPLSLPSQLGYLQKGFSHCFVAFKFAN 273

Query: 279 NPNFSSPLILGNLAVSSKDEHLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFN 338
           NPN SSPL++G+LA+SSKD HLQFTP+LKSP+YPNYYYIGLE +T+GN ++     V  +
Sbjct: 274 NPNISSPLVVGDLAISSKD-HLQFTPMLKSPMYPNYYYIGLEGLTVGNSSAT---KVPLS 333

Query: 339 LREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVP 398
           LRE D+ GNGGMLIDSGTTYTHLP+ LYSQL+S ++SVI YPRAK VE  TGF LCYK+P
Sbjct: 334 LREFDSLGNGGMLIDSGTTYTHLPQSLYSQLLSVIQSVIPYPRAKDVEARTGFGLCYKIP 393

Query: 399 CKNNN-----FSFIDDSQLPSITFHFLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSM 458
           C +NN     F+  DD  LP+ITFHFLNNVS++LPQ N+FYAM AP NSTVVKCLL QSM
Sbjct: 394 CTSNNNSSTIFTDHDDLLLPTITFHFLNNVSLLLPQGNHFYAMGAPSNSTVVKCLLLQSM 453

Query: 459 DGVGGDTDDDRDGPAGIFGSFQQQNLEVVYDLEKERLGFQPMDCAYVAATQGLHK 509
                D D++  GPAG+FGSFQQQN+EVVYDLEKER+GFQPMDCA  AA+QGLHK
Sbjct: 454 -----DDDENEYGPAGVFGSFQQQNVEVVYDLEKERIGFQPMDCASAAASQGLHK 490

BLAST of Bhi08G000771 vs. TrEMBL
Match: tr|V4TN99|V4TN99_9ROSI (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10017863mg PE=3 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 6.1e-181
Identity = 325/511 (63.60%), Postives = 397/511 (77.69%), Query Frame = 0

Query: 1   MASISTSFAKKILSYFLLLVYVSRKTLATNPKTNGPKDSLVIGLVHSRTTLLTPKKGYNF 60
           MA   ++ A  IL + L +     +TLAT  K NG K SLV+GL +SR +LL P    + 
Sbjct: 1   MAKAYSNIATIILLFLLSMSLTFHQTLATQ-KNNG-KHSLVLGLTNSRASLLIPSASKSS 60

Query: 61  ISRKRMKAMEMDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFD 120
           I +K  + ++M    +EPLRE+RDGYL+SL +GTP QVIQVYMDTGSDLTWVPCGNLSFD
Sbjct: 61  I-KKPSETLDM----MEPLREVRDGYLISLNIGTPTQVIQVYMDTGSDLTWVPCGNLSFD 120

Query: 121 CQDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATL 180
           C DC++Y+NN     ++ F P+ SS+S RDTC SSFC++IHSSDNPFDPCT++GCSL+TL
Sbjct: 121 CVDCDDYRNN---KLMSNFSPSRSSSSSRDTCASSFCLNIHSSDNPFDPCTMSGCSLSTL 180

Query: 181 VKATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCVGASY 240
           +K+TC RPCPSFAYTYG  G+V G LTRD L +H     S     ++ P+FCFGCVG++Y
Sbjct: 181 LKSTCCRPCPSFAYTYGEGGLVTGILTRDTLKVH----GSSPGIIREIPKFCFGCVGSTY 240

Query: 241 REPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSKDEHL 300
           REPIGIAGFGRG LS+P QLGF  KGFSHCFL FK++N+PN SSPL+LG++A+SSKD +L
Sbjct: 241 REPIGIAGFGRGALSVPSQLGFLQKGFSHCFLAFKYANDPNISSPLVLGDVAISSKD-NL 300

Query: 301 QFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTYTH 360
           QFTP+LKSP+YPNYYYIGLE+ITIGN +      V  +LRE D++GNGG+L+DSGTTYTH
Sbjct: 301 QFTPMLKSPMYPNYYYIGLEAITIGNSSLT---EVPLSLREFDSQGNGGLLVDSGTTYTH 360

Query: 361 LPEPLYSQLISNLESVIS-YPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITFHF 420
           LPEP YSQL+S L+S I+ YPRAK+VE  TGFDLCY+VPC NN F+   D   PSITFHF
Sbjct: 361 LPEPFYSQLLSILQSTITYYPRAKEVEERTGFDLCYRVPCPNNTFT---DDLFPSITFHF 420

Query: 421 LNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQNL 480
           LNNVS+VLPQ N+FYAM+AP NS+ VKCLLFQSM       DD   GP+G+FGSFQQQN+
Sbjct: 421 LNNVSLVLPQGNHFYAMSAPSNSSAVKCLLFQSM-------DDGDYGPSGVFGSFQQQNV 480

Query: 481 EVVYDLEKERLGFQPMDCAYVAATQGLHKNV 511
           EVVYDLEKER+GFQPMDCA  A+ QGLHK +
Sbjct: 481 EVVYDLEKERIGFQPMDCASTASAQGLHKKL 483

BLAST of Bhi08G000771 vs. TrEMBL
Match: tr|A0A2H5N1B2|A0A2H5N1B2_CITUN (Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_007680 PE=3 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 1.4e-180
Identity = 323/511 (63.21%), Postives = 396/511 (77.50%), Query Frame = 0

Query: 1   MASISTSFAKKILSYFLLLVYVSRKTLATNPKTNGPKDSLVIGLVHSRTTLLTPKKGYNF 60
           MA   ++ A  IL + L +     +TLAT  K NG K SLV+GL +SR +LL P      
Sbjct: 1   MAKAYSNIATIILLFLLYMSLRFHQTLATQ-KNNG-KHSLVLGLTNSRASLLIPS----- 60

Query: 61  ISRKRMKAMEMDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFD 120
            S+  +K      +++EPLRE+RDGYL+SL +GTP QVIQVYMDTGSDLTWVPCGNLSFD
Sbjct: 61  ASKSSVKKPSETLDMMEPLREVRDGYLISLNIGTPTQVIQVYMDTGSDLTWVPCGNLSFD 120

Query: 121 CQDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATL 180
           C DC++Y+NN     ++ F P+ SS+S RDTC SSFC++IHSSDNPFDPCT++GCSL+TL
Sbjct: 121 CVDCDDYRNN---KLMSNFSPSRSSSSSRDTCASSFCLNIHSSDNPFDPCTMSGCSLSTL 180

Query: 181 VKATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCVGASY 240
           +K+TC RPCPSFAYTYG  G+V G LTRD+L +H     S     ++ P+FCFGCVG++Y
Sbjct: 181 LKSTCCRPCPSFAYTYGEGGLVTGILTRDILKVH----GSSPGIIREIPKFCFGCVGSTY 240

Query: 241 REPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSKDEHL 300
           REPIGIAGFG+G LS+P QLGF  KGFSHCFL FK++N+PN SSPL+LG++A+SSKD +L
Sbjct: 241 REPIGIAGFGKGALSVPSQLGFLQKGFSHCFLAFKYANDPNISSPLVLGDVAISSKD-NL 300

Query: 301 QFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTYTH 360
           QFTP+LKSP+YPNYYYIGLE+ITIGN +      V  +LRE D++GNGG+L+DSGTTYTH
Sbjct: 301 QFTPMLKSPMYPNYYYIGLEAITIGNSSLT---EVPLSLREFDSQGNGGLLVDSGTTYTH 360

Query: 361 LPEPLYSQLISNLESVIS-YPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITFHF 420
           LPEP YSQL+S L+S I+ YPRAK+VE  TGFDLCY+VPC NN F+   D   PSITFHF
Sbjct: 361 LPEPFYSQLLSILQSTITYYPRAKEVEERTGFDLCYRVPCPNNTFT---DDLFPSITFHF 420

Query: 421 LNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQNL 480
           LNNVS+VLPQ N+FYAM+AP NS+ VKCLLFQSM       DD   GP+G+FGSFQQQN+
Sbjct: 421 LNNVSLVLPQGNHFYAMSAPSNSSAVKCLLFQSM-------DDGDYGPSGVFGSFQQQNV 480

Query: 481 EVVYDLEKERLGFQPMDCAYVAATQGLHKNV 511
           EVVYDLEKER+GFQPMDCA  A+ QGLHK +
Sbjct: 481 EVVYDLEKERIGFQPMDCASTASAQGLHKKL 483

BLAST of Bhi08G000771 vs. NCBI nr
Match: XP_008459091.1 (PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis melo])

HSP 1 Score: 923.7 bits (2386), Expect = 2.7e-265
Identity = 450/512 (87.89%), Postives = 471/512 (91.99%), Query Frame = 0

Query: 5   STSFAKKILSYFLLLVYVSRKTLATNPKTNGPKDSLVIGLVHSRTTLLTPKKGYNFISRK 64
           STS A K LS FLLLV+ S++TLATNPKTN PKDSLV+GLVHSRT+LLTPKKGYNFIS+K
Sbjct: 6   STSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYNFISKK 65

Query: 65  RMKAMEM---DDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDC 124
           RMKAM+    DDNVIEPLREIRDGYLMSL++GTPPQV+QVYMDTGSDLTWVPCGNLSFDC
Sbjct: 66  RMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGNLSFDC 125

Query: 125 QDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLV 184
           QDCEEYQNN+SGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLV
Sbjct: 126 QDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLV 185

Query: 185 KATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHIN---NINSPNSSTKKTPRFCFGCVGA 244
           K TCPRPCPSFAYTYGASGVV G+LTRDVL MH N               PRFCFGCVGA
Sbjct: 186 KGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYXXXXXXXXXXXXXVPRFCFGCVGA 245

Query: 245 SYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSKDE 304
           +YREPIGIAGFGRGLLSLPFQLGFS KGFSHCFLPFKFSNNPNFSSPLILG+LA+SSKDE
Sbjct: 246 TYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAISSKDE 305

Query: 305 HLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTY 364
           +LQFTPLLKSPIYPNYYYIGLESITIGNGN+NFRFGVSF LREIDTKGNGGMLIDSGTTY
Sbjct: 306 NLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTY 365

Query: 365 THLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITFH 424
           THLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNN SF+DDSQLPSITFH
Sbjct: 366 THLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLPSITFH 425

Query: 425 FLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQN 484
           FLNNVSVVLPQ NNFYAMAAPINSTVVKCLL+QSMDGVG D D D +GPAGIFGSFQQQN
Sbjct: 426 FLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQN 485

Query: 485 LEVVYDLEKERLGFQPMDCAYVAATQGLHKNV 511
           L+VVYDLEKERLGFQ MDC  VAA QGLHKNV
Sbjct: 486 LQVVYDLEKERLGFQAMDCVSVAANQGLHKNV 517

BLAST of Bhi08G000771 vs. NCBI nr
Match: XP_004145478.2 (PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis sativus] >KGN66888.1 hypothetical protein Csa_1G704590 [Cucumis sativus])

HSP 1 Score: 919.5 bits (2375), Expect = 5.1e-264
Identity = 448/514 (87.16%), Postives = 474/514 (92.22%), Query Frame = 0

Query: 1   MASIST-SFAKKILSYFLLLVYVSRKTLATNPKTNGPKDSLVIGLVHSRTTLLTPKKGYN 60
           M SIS+ S A K LS FLLLV+VS +TLATNPKTN PKDSLV+GLVHSRT+LLTPKKGYN
Sbjct: 1   MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  FISRKRMKAMEM---DDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120
           FIS+KRMKAM+    DDNVIEPLREIRDGYLMSL++GTPPQV+QVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNN+SGP+LAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCV 240
           LA+LVK TCPRPCPSFAYTYGASGVV G+LTRDVL  H  N         + PRFCFGCV
Sbjct: 181 LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTH-GNYXXXXXXXXQIPRFCFGCV 240

Query: 241 GASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSK 300
           GA+YREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLA+SSK
Sbjct: 241 GATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK 300

Query: 301 DEHLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGT 360
           DE+LQFTPLLKSP+YPNYYYIGLESITIGNG++NFRFGVSF LREIDTKGNGGMLIDSGT
Sbjct: 301 DENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGT 360

Query: 361 TYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSIT 420
           TYTHLPEPLYSQLISNLE VI YPRAKQVELNTGFDLCYKVPCKNNN SF+DD+QLPSIT
Sbjct: 361 TYTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSIT 420

Query: 421 FHFLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQ 480
           FHFLNNVSVVLPQ NNFYAMAAPINSTVVKCLL+QSMDGVG D D D +GPAGIFGSFQQ
Sbjct: 421 FHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQ 480

Query: 481 QNLEVVYDLEKERLGFQPMDCAYVAATQGLHKNV 511
           QN+EVVYDLEKERLGFQPMDC  VAA QGLHKNV
Sbjct: 481 QNIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNV 513

BLAST of Bhi08G000771 vs. NCBI nr
Match: XP_023520027.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 835.1 bits (2156), Expect = 1.3e-238
Identity = 421/512 (82.23%), Postives = 453/512 (88.48%), Query Frame = 0

Query: 1   MASISTSFAKKILSYFLLLVYVSRKTLA---TNPKTNGPKDSLVIGLVHSRTTLLTPKKG 60
           MASI    AK      L+LV VS + +     NPKT   KDSLV+GLVHSRT+LLTPK+G
Sbjct: 1   MASI---VAKSFFVLVLVLVLVSGEAMGQTLANPKTKFLKDSLVLGLVHSRTSLLTPKRG 60

Query: 61  YNFISRKRMKAMEM-DDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120
           YN +SRKR+K MEM +D+VIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN
Sbjct: 61  YNSLSRKRIKPMEMGNDDVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDC+EYQNNV GPKLAAFLPTHSSTSIRDTCGSSFC+DIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCDEYQNNVLGPKLAAFLPTHSSTSIRDTCGSSFCIDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCV 240
           LATLVK TCPRPCPSF+YTYGASG+VIGTLT+DV+ +H    NSPNSS +K P+FCFGCV
Sbjct: 181 LATLVKGTCPRPCPSFSYTYGASGLVIGTLTKDVIFIH---GNSPNSS-RKIPKFCFGCV 240

Query: 241 GASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSK 300
           GA+YREPIGIAGFGRGLLSLP+QLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLA+SSK
Sbjct: 241 GATYREPIGIAGFGRGLLSLPYQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK 300

Query: 301 DEHLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGT 360
            EHL+FTPLLKSP YPNYYYIGLESITIGNG +  RFGVS  LREIDTKGNGG+LIDSGT
Sbjct: 301 -EHLKFTPLLKSPFYPNYYYIGLESITIGNGENYSRFGVSLQLREIDTKGNGGILIDSGT 360

Query: 361 TYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSIT 420
           TYTHLPEPLYSQLISNLES+ISYPRAK+ ELNTGFDLCYKVP KNN F F D+ +LPSIT
Sbjct: 361 TYTHLPEPLYSQLISNLESLISYPRAKEHELNTGFDLCYKVPYKNNTF-FSDEFELPSIT 420

Query: 421 FHFLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQ 480
           FHFLNNVSVVLPQ N+FYAMAAP NSTVVKCLLFQSMDG       D DGPAGIFGSFQQ
Sbjct: 421 FHFLNNVSVVLPQGNSFYAMAAPSNSTVVKCLLFQSMDG-------DGDGPAGIFGSFQQ 480

Query: 481 QNLEVVYDLEKERLGFQPMDCAYVAATQGLHK 509
           QNLEVVYDLEKERLGF+ MDCA VA +QGLHK
Sbjct: 481 QNLEVVYDLEKERLGFEGMDCASVAVSQGLHK 496

BLAST of Bhi08G000771 vs. NCBI nr
Match: XP_022927421.1 (probable aspartyl protease At4g16563 [Cucurbita moschata])

HSP 1 Score: 826.6 bits (2134), Expect = 4.5e-236
Identity = 417/512 (81.45%), Postives = 450/512 (87.89%), Query Frame = 0

Query: 1   MASISTSFAKKILSYFLLLVYVSRKTLA---TNPKTNGPKDSLVIGLVHSRTTLLTPKKG 60
           MASI+   A+      L+LV VS + +     NPKT   KDSLV+GLVHSRT+LLTPK+G
Sbjct: 1   MASIA---ARSYFVLVLVLVLVSGEAMGQTLANPKTKFLKDSLVLGLVHSRTSLLTPKRG 60

Query: 61  YNFISRKRMKAMEM-DDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120
           YN +  KR+K MEM +D+VIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN
Sbjct: 61  YNSLLTKRIKPMEMGNDDVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDC+EYQNNV GPKLAAFLPTHSSTSIRDTCGSSFC+DIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCDEYQNNVLGPKLAAFLPTHSSTSIRDTCGSSFCIDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCV 240
           LATLVK TCPRPCPSF+YTYGASG+VIGTLT+DV+ +H    NSPNSS +K P+FCFGCV
Sbjct: 181 LATLVKGTCPRPCPSFSYTYGASGLVIGTLTKDVIFIH---GNSPNSS-RKIPKFCFGCV 240

Query: 241 GASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSK 300
           GA+YREPIGIAGFGRGLLSLP QLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLA+SSK
Sbjct: 241 GATYREPIGIAGFGRGLLSLPSQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK 300

Query: 301 DEHLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGT 360
            EHL+FTP LKSP YPNYYYIGLESITIGNG +  RFGVS  LREIDTKGNGG+LIDSGT
Sbjct: 301 -EHLKFTPFLKSPFYPNYYYIGLESITIGNGENYSRFGVSLQLREIDTKGNGGILIDSGT 360

Query: 361 TYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSIT 420
           TYTHLPEPLYSQLISNLES+ISYPRAK+ ELNTGFDLCYKVP KNN F F D+ +LPSIT
Sbjct: 361 TYTHLPEPLYSQLISNLESLISYPRAKEHELNTGFDLCYKVPYKNNTF-FSDEFELPSIT 420

Query: 421 FHFLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQ 480
           FHFLNNVSVVLPQ N+FYAMAAP NSTVVKCLLFQSMDG       D DGPAGIFGSFQQ
Sbjct: 421 FHFLNNVSVVLPQGNSFYAMAAPSNSTVVKCLLFQSMDG-------DGDGPAGIFGSFQQ 480

Query: 481 QNLEVVYDLEKERLGFQPMDCAYVAATQGLHK 509
           QNLEVVYDLEKERLGF+ MDCA VA +QGLHK
Sbjct: 481 QNLEVVYDLEKERLGFEAMDCASVAVSQGLHK 496

BLAST of Bhi08G000771 vs. NCBI nr
Match: XP_023000974.1 (probable aspartyl protease At4g16563 [Cucurbita maxima])

HSP 1 Score: 823.9 bits (2127), Expect = 2.9e-235
Identity = 411/498 (82.53%), Postives = 443/498 (88.96%), Query Frame = 0

Query: 15  YFLLLVYVSRKTLA---TNPKTNGPKDSLVIGLVHSRTTLLTPKKGYNFISRKRMKAMEM 74
           + L+LV VS + +     NPKT   KDSLV+GLVHSRT+LLTPK+GYN +SRKR+K MEM
Sbjct: 10  FVLVLVLVSGEAMGQTLANPKTKFLKDSLVLGLVHSRTSLLTPKRGYNSLSRKRIKPMEM 69

Query: 75  -DDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNN 134
            DD+VIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDC+EYQNN
Sbjct: 70  GDDDVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCDEYQNN 129

Query: 135 VSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKATCPRPCP 194
           V GPKLAAFLPTHSSTSIR+TCGSSFC+DIHSSDNPFDPCTIAGCSLATLVK  CPRPCP
Sbjct: 130 VLGPKLAAFLPTHSSTSIRETCGSSFCIDIHSSDNPFDPCTIAGCSLATLVKGACPRPCP 189

Query: 195 SFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCVGASYREPIGIAGFG 254
           SF+YTYGASG+VIGTLT+D + +H    NSPNSS +K P+FCFGCVGA+YREPIGIAGFG
Sbjct: 190 SFSYTYGASGLVIGTLTKDAIFIH---GNSPNSS-RKIPKFCFGCVGATYREPIGIAGFG 249

Query: 255 RGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSKDEHLQFTPLLKSPI 314
           RGLLSLP QLGFSHKGFSHCFLPFKFSNNP FSSPLILGNLA+SSK EHL+FTPLLKSP 
Sbjct: 250 RGLLSLPSQLGFSHKGFSHCFLPFKFSNNPKFSSPLILGNLAISSK-EHLKFTPLLKSPF 309

Query: 315 YPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLI 374
           YPNYYYIGLESITIGNG +  RFGVS  LREIDTKGNGG+LIDSGTTYTHLPEPLYSQLI
Sbjct: 310 YPNYYYIGLESITIGNGENYSRFGVSLQLREIDTKGNGGILIDSGTTYTHLPEPLYSQLI 369

Query: 375 SNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITFHFLNNVSVVLPQE 434
           S LES+ISYPRAK+ ELNTGFDLCYKVP KNN F F D+ +LPSITFHFLNNVSVVLPQ 
Sbjct: 370 SILESLISYPRAKEHELNTGFDLCYKVPYKNNTF-FSDEFELPSITFHFLNNVSVVLPQG 429

Query: 435 NNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQNLEVVYDLEKERL 494
           N+FYAMAAP NSTVVKCLLFQSMDG       D DGPAGIFGSFQQQNLEVVYDLEKERL
Sbjct: 430 NSFYAMAAPSNSTVVKCLLFQSMDG-------DGDGPAGIFGSFQQQNLEVVYDLEKERL 489

Query: 495 GFQPMDCAYVAATQGLHK 509
           GF+ MDCA VA +QGLHK
Sbjct: 490 GFEAMDCASVAVSQGLHK 494

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|Q940R4|ASP63_ARATH5.4e-4731.54Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656... [more]
sp|Q766C2|NEP2_NEPGR6.8e-3428.47Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
sp|Q9LNJ3|APF2_ARATH7.5e-3328.97Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
sp|Q766C3|NEP1_NEPGR4.1e-3129.12Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
sp|Q6XBF8|CDR1_ARATH1.5e-2828.51Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
AT5G45120.16.6e-16562.42Eukaryotic aspartyl protease family protein[more]
AT4G16563.13.0e-4831.54Eukaryotic aspartyl protease family protein[more]
AT3G52500.11.3e-4331.21Eukaryotic aspartyl protease family protein[more]
AT3G25700.14.3e-3928.38Eukaryotic aspartyl protease family protein[more]
AT1G01300.14.2e-3428.97Eukaryotic aspartyl protease family protein[more]
Match NameE-valueIdentityDescription
tr|A0A1S3CAK9|A0A1S3CAK9_CUCME1.8e-26587.89aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103498305 PE=3 S... [more]
tr|A0A0A0LYP0|A0A0A0LYP0_CUCSA3.4e-26487.16Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G704590 PE=3 SV=1[more]
tr|A0A2R6R206|A0A2R6R206_ACTCH1.1e-18267.37Aspartyl protease OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc1... [more]
tr|V4TN99|V4TN99_9ROSI6.1e-18163.60Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10017863mg PE=3 ... [more]
tr|A0A2H5N1B2|A0A2H5N1B2_CITUN1.4e-18063.21Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_007680 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008459091.12.7e-26587.89PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis melo][more]
XP_004145478.25.1e-26487.16PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis sativus] >KGN66888.1 hypot... [more]
XP_023520027.11.3e-23882.23probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo][more]
XP_022927421.14.5e-23681.45probable aspartyl protease At4g16563 [Cucurbita moschata][more]
XP_023000974.12.9e-23582.53probable aspartyl protease At4g16563 [Cucurbita maxima][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004190aspartic-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: INTERPRO
TermDefinition
IPR034161Pepsin-like_plant
IPR033121PEPTIDASE_A1
IPR001969Aspartic_peptidase_AS
IPR001461Aspartic_peptidase_A1
IPR032799TAXi_C
IPR032861TAXi_N
IPR021109Peptidase_aspartic_dom_sf
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030163 protein catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0004190 aspartic-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi08M000771Bhi08M000771mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3DG3DSA:2.40.70.10coord: 290..503
e-value: 5.0E-48
score: 165.1
IPR021109Aspartic peptidase domain superfamilyGENE3DG3DSA:2.40.70.10coord: 72..289
e-value: 1.4E-30
score: 108.7
IPR021109Aspartic peptidase domain superfamilySUPERFAMILYSSF50630Acid proteasescoord: 85..498
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 86..290
e-value: 1.6E-27
score: 96.8
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 315..493
e-value: 1.4E-27
score: 96.4
NoneNo IPR availablePANTHERPTHR13683:SF264ASPARTYL PROTEASE FAMILY PROTEINcoord: 15..501
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 15..501
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 350..361
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 86..493
score: 33.527
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 85..497
e-value: 1.93173E-71
score: 229.842

The following gene(s) are paralogous to this gene:

None