Bhi08G000351 (gene) Wax gourd

NameBhi08G000351
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionCation/H(+) antiporter like
Locationchr8 : 14917230 .. 14919989 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTACTCAAGCAGCACATAATGGAAGCTGGGTGTGCCAGCCTAATCGGCATTACAGATCCAGAGGCCTTTTCTTTGGTGACAGCCCTTTCTCCTTTGCAGATTCCGTTCTCCTCGCTCAGCTCTCTCTCTCTTCTCTCCTTACCTCTCTTTTACAATGCCTTCTCACTCCTTTGGGGGAGAGCTCCTTCATCTCTCAGATGCTGGTACGTAATTTAGGTTACAACTGATCATTGTTTTGTTCTTATATATATTTTTTTTATATTAATGGAGGAATATAATGAATAAAATCAGGTGGGTCTTGCGCTTGGGCCATCCTTCTACGGAGGAGACAGCCCAATTCTGGAGGCGATATTTCCATTCAAAAGCTTCTACGTGAGCGAAACATTTGCTTACTTTGGGTGCATGGTGTTCATGTTTCTTGTTGGGGTTAAAATGGATTTGAGCTTAATCGCAAAATCAGGGAAAAAGGCGTTGGTGATCGGAGTGTTGGCATTTGTAACCCCAATGGTTTTCAACTTCTTGTTAACTACGTATTTGAAGAGCAATGTGGAGATGGATGCAAACTTGAAAAACTTCCTCACAACCATTGGTGCCTTTCATGCCTCCAGCTCCTTCCATGTCATTGCTTGCCTTTTAAGCGACCTCAAACTCCTCAACTCCGACATCGGCCGCCTCGCCCTCTCTTCCTCCATGATCAGCGGCACCCTTAGTTGGATCGGCCTTGTGGCCAGCTTCACAATGCGTCAAACTTCGATGCAGCAACAAGACGTTCTTCCATGGATGGCCCTTTGCGTGGTGTGTATGATCATTCTTGTGGTTTATATTCTGCGCCCCATCATGATTTGGATCATGGAACAGACCAATCTCTCCGGTAGGCCCATCAAGGAGACCTACGTTTTATCTTTGTTCTTGATGTTGTTGTTCTGTGCTCTCTTTAGTGAGTTCGTGGGCCAACATTTCATTCTCGGCCCAATGATTCTAGGCCTCGCTGTCCCTGATGGGCCGCCTCTAGGGTCGGCCTTGGTCGATAAACTGGACTCCTTTGTTTCCTCCATTATGCTGCCATGCTTCTTTGTGATCAGTGGGGCCAGAATGAATCTCTCCATGTTTGATATGAGGAGCGCTTCCATTATTCATCTCTTGGCCTTCACCGCATTTATGGGCAAGCTTATTGGGACAATGCTGCCTTCTTTGTACTGTAAAATGTCATTGGTTGATTCTTTGTCCTTGGGACTCATCATGAGCGCTCAAGGCATTGCTGATATTCTCGGTCTCCAACATGGATTGCTCCTCTATGTAATTAACTTAATCTCATTGATCTCCTTTTAATTAAATTCTCTAATTTCCATTTATTTATATTAATTAATTTTTGTAGATGATAGATCAGAGTTCATACAGCATGACAGTGGTGGCCATGATGGTGATGACAGGCACAATATGCCCAATAGTGAAAATATTATACAACCCATCAAAGAGATACAGATGCAGTGCGAGGAGGAGGACCATCGAGCATACGAGCGCCAACGCAGAGTTACGTCTGTTGCTTTGCATTCACCACCAAGACGACACTCCCTCCATAATCAACATGCTCGAGCTCTCCAATCCTACCATTAAAAGCCCCATTTGCTTCTACCTAATCCACCTCCTCCAACTCACCGGCCGAGCCTCCCCCCTCCTCATCCACCACCATCATCTCCGTCAACGTGGCTCCAAACACTACAAGCTCTCCGATCAAATCATTAAAGCCTTCCAAATCTATCAGCAATTCAACTACGACAAGGTTTTTATATTTCAAATCCGAACTTTCACTTTTCCCTGCTAGCTTTTAAATAAACTTTGCATTATTTCTTTTTACAAATCTGCAATAGTGTCTACACTTTTGTCTGAATTCAACAAATGGGGGTTAATAATACGTGGTCTAGGTTATAATGAACGCGTTCACATCGGTGTCCCCGTATGCCACCATGCATGACGACGTGTGCATGTTGGCGTTAGAGAAGCGGGTGGCAATGGTGATCGTGCCGTTCCACAAGCGGAGGACGATCAACGGCATAGTGGAATCAATAAATCCAATAAGGGGAGTGAACAAGAACATTTTGTCAAAGGCTCCTTGTTCGGTCGGGATCTTGATAGACCGTGAGATGTTGCCGAGCACGGCGGCATCAGTGTCGTTGATGAACAGAGTGGATTTGTATAAGGTGGGTATGATCTTTGTGGAAGGCCCCGATGACCGGGAGGCACTGGCGTATGCCACTCGCATGGCGGAGCATCCAATGGTGGCACTCACGGTGGTTCGAGTGATCCAGCCGAAGCGAAGCAGCCGGCACCCAGCGGACCAAGACCTGGACGCGGAGATGATAAATGAGTTCAAGTTAATCATGGAAACCTCAGTGCTTAAGCACTGCACTTACGAAGAGGAAATGGCAAGCGATTGCGTGGGGCTCATAAACGTGATAAGAACAATGGAACACGATTACGATATGATCTTGGTGGGGCGCCGCCACGACGGCGACTCGGCCCTGTTTGTGGGGTTGCACGAGTGGAACGAGTTTCCTGAACTGGGGTACATTGGAGACATGTTGGCATCCTCGGACTCCACCGGTGCCGTCGCAGTGCTGGTGGTTCAGCAAGCAGACCATCGGCGGAGATCAGGAGTTTTTGGACGATTTCCGATGCCTCATGGAGGAGTCGTTTTCTGTGGACATAAGGCCCCTCAACCTTCCAACTGCATGGCCCCAAAACCTTCGGTTAACTGA

mRNA sequence

ATGAGTACTCAAGCAGCACATAATGGAAGCTGGGTGTGCCAGCCTAATCGGCATTACAGATCCAGAGGCCTTTTCTTTGGTGACAGCCCTTTCTCCTTTGCAGATTCCGTTCTCCTCGCTCAGCTCTCTCTCTCTTCTCTCCTTACCTCTCTTTTACAATGCCTTCTCACTCCTTTGGGGGAGAGCTCCTTCATCTCTCAGATGCTGGTGGGTCTTGCGCTTGGGCCATCCTTCTACGGAGGAGACAGCCCAATTCTGGAGGCGATATTTCCATTCAAAAGCTTCTACGTGAGCGAAACATTTGCTTACTTTGGGTGCATGGTGTTCATGTTTCTTGTTGGGGTTAAAATGGATTTGAGCTTAATCGCAAAATCAGGGAAAAAGGCGTTGGTGATCGGAGTGTTGGCATTTGTAACCCCAATGGTTTTCAACTTCTTGTTAACTACGTATTTGAAGAGCAATGTGGAGATGGATGCAAACTTGAAAAACTTCCTCACAACCATTGGTGCCTTTCATGCCTCCAGCTCCTTCCATGTCATTGCTTGCCTTTTAAGCGACCTCAAACTCCTCAACTCCGACATCGGCCGCCTCGCCCTCTCTTCCTCCATGATCAGCGGCACCCTTAGTTGGATCGGCCTTGTGGCCAGCTTCACAATGCGTCAAACTTCGATGCAGCAACAAGACGTTCTTCCATGGATGGCCCTTTGCGTGGTGTGTATGATCATTCTTGTGGTTTATATTCTGCGCCCCATCATGATTTGGATCATGGAACAGACCAATCTCTCCGGTAGGCCCATCAAGGAGACCTACGTTTTATCTTTGTTCTTGATGTTGTTGTTCTGTGCTCTCTTTAGTGAGTTCGTGGGCCAACATTTCATTCTCGGCCCAATGATTCTAGGCCTCGCTGTCCCTGATGGGCCGCCTCTAGGGTCGGCCTTGGTCGATAAACTGGACTCCTTTGTTTCCTCCATTATGCTGCCATGCTTCTTTGTGATCAGTGGGGCCAGAATGAATCTCTCCATGTTTGATATGAGGAGCGCTTCCATTATTCATCTCTTGGCCTTCACCGCATTTATGGGCAAGCTTATTGGGACAATGCTGCCTTCTTTGTACTGTAAAATGTCATTGGTTGATTCTTTGTCCTTGGGACTCATCATGAGCGCTCAAGGCATTGCTGATATTCTCGGTCTCCAACATGGATTGCTCCTCTATATGATAGATCAGAGTTCATACAGCATGACAGTGGTGGCCATGATGGTGATGACAGGCACAATATGCCCAATAGTGAAAATATTATACAACCCATCAAAGAGATACAGATGCAGTGCGAGGAGGAGGACCATCGAGCATACGAGCGCCAACGCAGAGTTACGTCTGTTGCTTTGCATTCACCACCAAGACGACACTCCCTCCATAATCAACATGCTCGAGCTCTCCAATCCTACCATTAAAAGCCCCATTTGCTTCTACCTAATCCACCTCCTCCAACTCACCGGCCGAGCCTCCCCCCTCCTCATCCACCACCATCATCTCCGTCAACGTGGCTCCAAACACTACAAGCTCTCCGATCAAATCATTAAAGCCTTCCAAATCTATCAGCAATTCAACTACGACAAGGTTATAATGAACGCGTTCACATCGGTGTCCCCGTATGCCACCATGCATGACGACGTGTGCATGTTGGCGTTAGAGAAGCGGGTGGCAATGGTGATCGTGCCGTTCCACAAGCGGAGGACGATCAACGGCATAGTGGAATCAATAAATCCAATAAGGGGAGTGAACAAGAACATTTTGTCAAAGGCTCCTTGTTCGGTCGGGATCTTGATAGACCGTGAGATGTTGCCGAGCACGGCGGCATCAGTGTCGTTGATGAACAGAGTGGATTTGTATAAGGTGGGTATGATCTTTGTGGAAGGCCCCGATGACCGGGAGGCACTGGCGTATGCCACTCGCATGGCGGAGCATCCAATGGTGGCACTCACGGTGGTTCGAGTGATCCAGCCGAAGCGAAGCAGCCGGCACCCAGCGGACCAAGACCTGGACGCGGAGATGATAAATGAGTTCAAGTTAATCATGGAAACCTCAGTGCTTAAGCACTGCACTTACGAAGAGGAAATGGCAAGCGATTGCGTGGGGCTCATAAACGTGATAAGAACAATGGAACACGATTACGATATGATCTTGGTGGGGCGCCGCCACGACGGCGACTCGGCCCTGTTTGTGGGGTTGCACGAGTGGAACGAGTTTCCTGAACTGGGGTACATTGGAGACATGTTGGCATCCTCGGACTCCACCGGTGCCGTCGCAGTGCTGGTGGTTCAGCAAGCAGACCATCGGCGGAGATCAGGAGTTTTTGGACGATTTCCGATGCCTCATGGAGGAGTCGTTTTCTGTGGACATAAGGCCCCTCAACCTTCCAACTGCATGGCCCCAAAACCTTCGGTTAACTGA

Coding sequence (CDS)

ATGAGTACTCAAGCAGCACATAATGGAAGCTGGGTGTGCCAGCCTAATCGGCATTACAGATCCAGAGGCCTTTTCTTTGGTGACAGCCCTTTCTCCTTTGCAGATTCCGTTCTCCTCGCTCAGCTCTCTCTCTCTTCTCTCCTTACCTCTCTTTTACAATGCCTTCTCACTCCTTTGGGGGAGAGCTCCTTCATCTCTCAGATGCTGGTGGGTCTTGCGCTTGGGCCATCCTTCTACGGAGGAGACAGCCCAATTCTGGAGGCGATATTTCCATTCAAAAGCTTCTACGTGAGCGAAACATTTGCTTACTTTGGGTGCATGGTGTTCATGTTTCTTGTTGGGGTTAAAATGGATTTGAGCTTAATCGCAAAATCAGGGAAAAAGGCGTTGGTGATCGGAGTGTTGGCATTTGTAACCCCAATGGTTTTCAACTTCTTGTTAACTACGTATTTGAAGAGCAATGTGGAGATGGATGCAAACTTGAAAAACTTCCTCACAACCATTGGTGCCTTTCATGCCTCCAGCTCCTTCCATGTCATTGCTTGCCTTTTAAGCGACCTCAAACTCCTCAACTCCGACATCGGCCGCCTCGCCCTCTCTTCCTCCATGATCAGCGGCACCCTTAGTTGGATCGGCCTTGTGGCCAGCTTCACAATGCGTCAAACTTCGATGCAGCAACAAGACGTTCTTCCATGGATGGCCCTTTGCGTGGTGTGTATGATCATTCTTGTGGTTTATATTCTGCGCCCCATCATGATTTGGATCATGGAACAGACCAATCTCTCCGGTAGGCCCATCAAGGAGACCTACGTTTTATCTTTGTTCTTGATGTTGTTGTTCTGTGCTCTCTTTAGTGAGTTCGTGGGCCAACATTTCATTCTCGGCCCAATGATTCTAGGCCTCGCTGTCCCTGATGGGCCGCCTCTAGGGTCGGCCTTGGTCGATAAACTGGACTCCTTTGTTTCCTCCATTATGCTGCCATGCTTCTTTGTGATCAGTGGGGCCAGAATGAATCTCTCCATGTTTGATATGAGGAGCGCTTCCATTATTCATCTCTTGGCCTTCACCGCATTTATGGGCAAGCTTATTGGGACAATGCTGCCTTCTTTGTACTGTAAAATGTCATTGGTTGATTCTTTGTCCTTGGGACTCATCATGAGCGCTCAAGGCATTGCTGATATTCTCGGTCTCCAACATGGATTGCTCCTCTATATGATAGATCAGAGTTCATACAGCATGACAGTGGTGGCCATGATGGTGATGACAGGCACAATATGCCCAATAGTGAAAATATTATACAACCCATCAAAGAGATACAGATGCAGTGCGAGGAGGAGGACCATCGAGCATACGAGCGCCAACGCAGAGTTACGTCTGTTGCTTTGCATTCACCACCAAGACGACACTCCCTCCATAATCAACATGCTCGAGCTCTCCAATCCTACCATTAAAAGCCCCATTTGCTTCTACCTAATCCACCTCCTCCAACTCACCGGCCGAGCCTCCCCCCTCCTCATCCACCACCATCATCTCCGTCAACGTGGCTCCAAACACTACAAGCTCTCCGATCAAATCATTAAAGCCTTCCAAATCTATCAGCAATTCAACTACGACAAGGTTATAATGAACGCGTTCACATCGGTGTCCCCGTATGCCACCATGCATGACGACGTGTGCATGTTGGCGTTAGAGAAGCGGGTGGCAATGGTGATCGTGCCGTTCCACAAGCGGAGGACGATCAACGGCATAGTGGAATCAATAAATCCAATAAGGGGAGTGAACAAGAACATTTTGTCAAAGGCTCCTTGTTCGGTCGGGATCTTGATAGACCGTGAGATGTTGCCGAGCACGGCGGCATCAGTGTCGTTGATGAACAGAGTGGATTTGTATAAGGTGGGTATGATCTTTGTGGAAGGCCCCGATGACCGGGAGGCACTGGCGTATGCCACTCGCATGGCGGAGCATCCAATGGTGGCACTCACGGTGGTTCGAGTGATCCAGCCGAAGCGAAGCAGCCGGCACCCAGCGGACCAAGACCTGGACGCGGAGATGATAAATGAGTTCAAGTTAATCATGGAAACCTCAGTGCTTAAGCACTGCACTTACGAAGAGGAAATGGCAAGCGATTGCGTGGGGCTCATAAACGTGATAAGAACAATGGAACACGATTACGATATGATCTTGGTGGGGCGCCGCCACGACGGCGACTCGGCCCTGTTTGTGGGGTTGCACGAGTGGAACGAGTTTCCTGAACTGGGGTACATTGGAGACATGTTGGCATCCTCGGACTCCACCGGTGCCGTCGCAGTGCTGGTGGTTCAGCAAGCAGACCATCGGCGGAGATCAGGAGTTTTTGGACGATTTCCGATGCCTCATGGAGGAGTCGTTTTCTGTGGACATAAGGCCCCTCAACCTTCCAACTGCATGGCCCCAAAACCTTCGGTTAACTGA

Protein sequence

MSTQAAHNGSWVCQPNRHYRSRGLFFGDSPFSFADSVLLAQLSLSSLLTSLLQCLLTPLGESSFISQMLVGLALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLSLIAKSGKKALVIGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVIACLLSDLKLLNSDIGRLALSSSMISGTLSWIGLVASFTMRQTSMQQQDVLPWMALCVVCMIILVVYILRPIMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILGLAVPDGPPLGSALVDKLDSFVSSIMLPCFFVISGARMNLSMFDMRSASIIHLLAFTAFMGKLIGTMLPSLYCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMVMTGTICPIVKILYNPSKRYRCSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSNPTIKSPICFYLIHLLQLTGRASPLLIHHHHLRQRGSKHYKLSDQIIKAFQIYQQFNYDKVIMNAFTSVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESINPIRGVNKNILSKAPCSVGILIDREMLPSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAEHPMVALTVVRVIQPKRSSRHPADQDLDAEMINEFKLIMETSVLKHCTYEEEMASDCVGLINVIRTMEHDYDMILVGRRHDGDSALFVGLHEWNEFPELGYIGDMLASSDSTGAVAVLVVQQADHRRRSGVFGRFPMPHGGVVFCGHKAPQPSNCMAPKPSVN
BLAST of Bhi08G000351 vs. Swiss-Prot
Match: sp|Q9SIT5|CHX15_ARATH (Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 2.5e-131
Identity = 289/798 (36.22%), Postives = 477/798 (59.77%), Query Frame = 0

Query: 2   STQAAHNGSWVCQPNRHYRSRGLFFGDSPFSFADSVLLAQLSLSSLLTSLLQCLLTPLGE 61
           S + + + S +C       + G++ GD+P  F+  + + QL+L  ++T     +L P  +
Sbjct: 4   SEEPSTDASIICYAPSMITTNGVWQGDNPLDFSLPLFVLQLTLVVVVTRFFVFILKPFRQ 63

Query: 62  SSFISQMLVGLALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLSL 121
              IS++L G+ LGPS  G  +     IFP +S  V ET A  G + F+FLVGV+MD+ +
Sbjct: 64  PRVISEILGGIVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLVGVEMDIMV 123

Query: 122 IAKSGKKALVIGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVIA 181
           + K+GK+AL I +   V P +      ++     E       ++  +G   + ++F V+A
Sbjct: 124 VRKTGKRALTIAIGGMVLPFLIGAAF-SFSMHRSEDHLGQGTYILFLGVALSVTAFPVLA 183

Query: 182 CLLSDLKLLNSDIGRLALSSSMISGTLSWIGLVASFTMRQTSMQQQDVLPWMALCVVCMI 241
            +L++LKL+N++IGR+++S+++++   +WI L  +  + ++       L W+ +     I
Sbjct: 184 RILAELKLINTEIGRISMSAALVNDMFAWILLALAIALAESDKTSFASL-WVMISSAVFI 243

Query: 242 ILVVYILRPIMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILGL 301
            + V+++RP + WI+ +T   G    E ++  +   ++     ++ +G H + G  + GL
Sbjct: 244 AVCVFVVRPGIAWIIRKTP-EGENFSEFHICLILTGVMISGFITDAIGTHSVFGAFVFGL 303

Query: 302 AVPDGPPLGSALVDKLDSFVSSIMLPCFFVISGARMNLSMFDMRSASI-IHLLAFTAFMG 361
            +P+G PLG  L++KL+ FVS ++LP FF ISG + N++     +  + + L+ F A  G
Sbjct: 304 VIPNG-PLGLTLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLTLFLVIFLACAG 363

Query: 362 KLIGTMLPSLYCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMV 421
           K+IGT++ + +  M + + ++LGL+++ +G+ +++ L  G    ++D  +++  V+  +V
Sbjct: 364 KVIGTVIVAFFHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFATMVLVALV 423

Query: 422 MTGTICPIVKILYNPSKRYRCSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSN 481
           MTG I PIV ILY P K+   S +RRTI+ T  ++ELR+L+C+H   + P+IIN+LE S+
Sbjct: 424 MTGVITPIVTILYKPVKK-SVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIINLLEASH 483

Query: 482 PTIKSPICFYLIHLLQLTGRASPLLIHHHHLRQRG----SKHYKLSDQIIKAFQIYQQFN 541
           PT +SPIC Y++HL++LTGRAS +LI  H+ R+ G    ++    SD II AF+ Y+Q +
Sbjct: 484 PTKRSPICIYVLHLVELTGRASAMLI-VHNTRKSGRPALNRTQAQSDHIINAFENYEQ-H 543

Query: 542 YDKVIMNAFTSVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESINP-IRGVNK 601
              V +   T++SPY+TMH+DVC LA +KRV+ +I+PFHK++T++G +ES NP  R VN+
Sbjct: 544 AAFVAVQPLTAISPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAYRLVNQ 603

Query: 602 NILSKAPCSVGILIDREMLPSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAEH 661
           N+L  +PCSVGIL+DR +  +T  +    N V L +V ++F  GPDDREALAYA RMA+H
Sbjct: 604 NLLENSPCSVGILVDRGLNGATRLN---SNTVSL-QVAVLFFGGPDDREALAYAWRMAQH 663

Query: 662 PMVALTVVRVIQPK-----RSSRHPADQD-------------LDAEMINEFKLIMETSVL 721
           P + LTV+R I  +      S+R   D D             LD + IN F+   E +  
Sbjct: 664 PGITLTVLRFIHDEDEADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFR--AENAEY 723

Query: 722 KHCTYEEEMASDCVGLINVIRTMEHDYDMILVGRRHDGDSALFVGLHEWNEFPELGYIGD 776
           +   Y E++ S+    +  +R+M+  +D+ +VGR     S L  GL +W+E PELG IGD
Sbjct: 724 ESIVYIEKLVSNGEETVAAVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGD 783

BLAST of Bhi08G000351 vs. Swiss-Prot
Match: sp|Q9LMJ1|CHX14_ARATH (Cation/H(+) antiporter 14 OS=Arabidopsis thaliana OX=3702 GN=CHX14 PE=2 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 6.2e-130
Identity = 268/788 (34.01%), Postives = 454/788 (57.61%), Query Frame = 0

Query: 12  VCQPNRHYRSRGLFFGDSPFSFADSVLLAQLSLSSLLTSLLQCLLTPLGESSFISQMLVG 71
           VCQ N    S+G+F G  P  +A  ++L Q+S+  + + LL  LL PL +    +Q+L G
Sbjct: 27  VCQKNHMLTSKGVFLGSDPLKYAMPLMLLQMSVIIITSRLLYRLLKPLKQGMISAQVLAG 86

Query: 72  LALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLSLIAKSGKKALV 131
           + LGPS +G  S  ++   P       +T +  G  + +FL+G+++D S+I K+G KA++
Sbjct: 87  IILGPSLFGQSSAYMQMFLPISGKITLQTLSNLGFFIHLFLLGLRIDASIIRKAGSKAIL 146

Query: 132 IGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVIACLLSDLKLLN 191
           IG  ++  P     L   +LK+   +  ++ + ++T+ + +A +SF V   +L++L +LN
Sbjct: 147 IGTASYALPFSLGNLTVLFLKNTYNLPPDVVHCISTVISLNAMTSFPVTTTVLAELNILN 206

Query: 192 SDIGRLALSSSMISGTLSWI-GLVASFTMRQTSMQQQDVLPWMALCVVCMIILVVYILRP 251
           SD+GRLA + S++    SWI  LV    +R  ++       W  + V  +I+++ ++ RP
Sbjct: 207 SDLGRLATNCSIVCEAFSWIVALVFRMFLRDGTLAS----VWSFVWVTALILVIFFVCRP 266

Query: 252 IMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILGLAVPDGPPLG 311
            +IW+ E+ ++S     E     + ++LL  +L SE +G H   G   LG+++PDGPPLG
Sbjct: 267 AIIWLTERRSISIDKAGEIPFFPIIMVLLTISLTSEVLGVHAAFGAFWLGVSLPDGPPLG 326

Query: 312 SALVDKLDSFVSSIMLPCFFVISGARMNLSMFDMRSASIIHLLAFTAFMGKLIGTMLPSL 371
           + L  KL+ F +S+MLPCF  ISG + N  +       II  +    +  K +GT   S 
Sbjct: 327 TGLTTKLEMFATSLMLPCFISISGLQTNFFIIGESHVKIIEAVILITYGCKFLGTAAASA 386

Query: 372 YCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMVMTGTICPIVK 431
           YC + + D+ SL L+M  QG+ +I          +++   +++ ++ ++++TG    +V 
Sbjct: 387 YCNIQIGDAFSLALLMCCQGVIEIYTCVMWKDEKVLNTECFNLLIITLLLVTGISRFLVV 446

Query: 432 ILYNPSKRYRCSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSNPTIKSPICFY 491
            LY+PSKRYR  ++R  ++    N + RLLLC+++ ++ PS++N+LE S P+  SPI  +
Sbjct: 447 CLYDPSKRYRSKSKRTILDTRQRNLQFRLLLCVYNVENVPSMVNLLEASYPSRFSPISVF 506

Query: 492 LIHLLQLTGRASPLLIHHHHLRQRGSKHYKLSDQIIKAFQIYQQFNYDKVIMNAFTSVSP 551
            +HL++L GRA  +L+ HH + +      + S  I+  FQ ++Q N   ++   FT+ +P
Sbjct: 507 TLHLVELKGRAHAVLVPHHQMNKLDPNTVQ-STHIVNGFQRFEQQNQGTLMAQHFTAAAP 566

Query: 552 YATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESINP-IRGVNKNILSKAPCSVGILI 611
           +++++DD+C LAL+K+  ++++PFHK+  I+G V+ +NP IR +N N+L KAPCSVGI I
Sbjct: 567 FSSINDDICTLALDKKATLIVIPFHKQYAIDGTVDHVNPSIRNINLNVLEKAPCSVGIFI 626

Query: 612 DREMLPSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAEHPMVALTVVRVIQPK 671
           DR     T    S++       V +IF+EG DD EALA++ R+AEHP V++T++   + K
Sbjct: 627 DR---GETEGRRSVLMSYTWRNVAVIFIEGRDDAEALAFSMRIAEHPEVSVTMIH-FRHK 686

Query: 672 RSSRHPADQDLDAE-----MINEFKLIMETSVLKHCTYEEEMASDCVGLINVIRTMEHDY 731
            S +     D+++E     +IN+FK    +      +Y EE+  D V    VI ++   +
Sbjct: 687 SSLQQNHVVDVESELAESYLINDFKNFAMSK--PKISYREEIVRDGVETTQVISSLGDSF 746

Query: 732 DMILVGRRHDGDSALFVGLHEWNEFPELGYIGDMLASSDSTGAVAVLVVQQADHRRRSGV 791
           D+++VGR HD +S++  GL +W+E PELG IGDM ASSD   +V V+  Q+ D       
Sbjct: 747 DLVVVGRDHDLESSVLYGLTDWSECPELGVIGDMFASSDFHFSVLVIHQQEGDSLAMDNS 803

Query: 792 FGRFPMPH 793
           +     PH
Sbjct: 807 YKLPASPH 803

BLAST of Bhi08G000351 vs. Swiss-Prot
Match: sp|O22920|CHX13_ARATH (Cation/H(+) symporter 13 OS=Arabidopsis thaliana OX=3702 GN=CHX13 PE=1 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 2.2e-111
Identity = 257/777 (33.08%), Postives = 429/777 (55.21%), Query Frame = 0

Query: 12  VCQPNRHYRSRGLFFGDSPFSFADSVLLAQLSLSSLLTSLLQCLLTPLGESSFISQMLVG 71
           VCQ      SRG+F   +P  +A  +LL Q+S+  + + L+  +L PL +    +Q+L G
Sbjct: 29  VCQAQNMLTSRGIFMKSNPLKYALPLLLLQMSVIIVTSRLIFRVLQPLKQGMISAQVLTG 88

Query: 72  LALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLSLIAKSGKKALV 131
           + LGPSF G +   +    P     + +T +  G ++ +FL+G+K+D S+I K+G KA++
Sbjct: 89  VVLGPSFLGHNVIYMNMFLPAGGKIIIQTLSNVGFVIHLFLLGLKIDGSIIRKAGSKAIL 148

Query: 132 IGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVIACLLSDLKLLN 191
           IG  ++  P     L   ++   + + +++ +  ++  +  + +SF V   +L++L +LN
Sbjct: 149 IGTASYAFPFSLGNLTIMFISKTMGLPSDVISCTSSAISLSSMTSFPVTTTVLAELNILN 208

Query: 192 SDIGRLALSSSMISGTLSW-IGLVASFTMRQTSMQQQDVLPWMALCVVCMIILVVY-ILR 251
           S++GRLA   SM+    SW + L  +   R  +M         AL ++  ++LV+Y + R
Sbjct: 209 SELGRLATHCSMVCEVCSWFVALAFNLYTRDRTMTS-----LYALSMIIGLLLVIYFVFR 268

Query: 252 PIMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILGLAVPDGPPL 311
           PI++W+ ++   S           + L+L   +L  E +G H   G   LG+++PDGPPL
Sbjct: 269 PIIVWLTQRKTKSMDKKDVVPFFPVLLLLSIASLSGEAMGVHAAFGAFWLGVSLPDGPPL 328

Query: 312 GSALVDKLDSFVSSIMLPCFFVISGARMN---LSMFDMRSASIIHLLAFTAFMGKLIGTM 371
           G+ L  KL+ F S++ LPCF  ISG + N   ++        +I ++    +  K +GT 
Sbjct: 329 GTELAAKLEMFASNLFLPCFIAISGLQTNFFEITESHEHHVVMIEIILLITYGCKFLGTA 388

Query: 372 LPSLYCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMVMTGTIC 431
             S YC+  + D+L L  +M  QGI ++          ++D   +++ ++ ++ +TG   
Sbjct: 389 AASAYCQTQIGDALCLAFLMCCQGIIEVYTTIVWKDAQVVDTECFNLVIITILFVTGISR 448

Query: 432 PIVKILYNPSKRYRCSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSNPTIKSP 491
            +V  LY+PSKRY+  ++R  +     N +LRLLL +++ ++ PS++N+LE + PT  +P
Sbjct: 449 FLVVYLYDPSKRYKSKSKRTILNTRQHNLQLRLLLGLYNVENVPSMVNLLEATYPTRFNP 508

Query: 492 ICFYLIHLLQLTGRASPLLIHHHHLRQRGSKHYKLSDQIIKAFQIYQQFNYDKVIMNAFT 551
           I F+ +HL++L GRA  LL  HH + +    +   S  I+ AFQ ++Q     ++   FT
Sbjct: 509 ISFFTLHLVELKGRAHALLTPHHQMNKL-DPNTAQSTHIVNAFQRFEQKYQGALMAQHFT 568

Query: 552 SVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESIN-PIRGVNKNILSKAPCSV 611
           + +PY+++++D+C LAL+K+  ++++PFHK+  I+G V  +N PIR +N N+L  APCSV
Sbjct: 569 AAAPYSSINNDICTLALDKKATLIVIPFHKQYAIDGTVGQVNGPIRTINLNVLDAAPCSV 628

Query: 612 GILIDREMLPSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAEHPMVALTVVRV 671
            I IDR     T    S++       V M+F+ G DD EALA   RMAE P + +T++  
Sbjct: 629 AIFIDR---GETEGRRSVLMTNTWQNVAMLFIGGKDDAEALALCMRMAEKPDLNVTMIHF 688

Query: 672 IQPKRSSRH-PADQDLDAEMINEFKLIME----TSVLKHCTYEEEMASDCVGLINVIRTM 731
                  RH  A QD D   ++E+ LI +     +      Y EE+  D V    VI ++
Sbjct: 689 -------RHKSALQDEDYSDMSEYNLISDFKSYAANKGKIHYVEEIVRDGVETTQVISSL 748

Query: 732 EHDYDMILVGRRHDGDSALFVGLHEWNEFPELGYIGDMLASSDSTGAVAVLVVQQAD 778
              YDM+LVGR HD +S++  GL +W+E PELG IGDML S D   +V V+  QQ D
Sbjct: 749 GDAYDMVLVGRDHDLESSVLYGLTDWSECPELGVIGDMLTSPDFHFSVLVVHQQQGD 789

BLAST of Bhi08G000351 vs. Swiss-Prot
Match: sp|Q9FFR9|CHX18_ARATH (Cation/H(+) antiporter 18 OS=Arabidopsis thaliana OX=3702 GN=CHX18 PE=2 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 2.9e-103
Identity = 252/797 (31.62%), Postives = 440/797 (55.21%), Query Frame = 0

Query: 6   AHNGSWVCQPNRHYRSRGLFFGDSPFSFADSVLLAQLSLSSLLTSLLQCLLTPLGESSFI 65
           A N +  C       S G+F GD+P  FA  + + Q+               PL +   I
Sbjct: 2   ATNSTKACPAPMKATSNGVFQGDNPIDFALPLAILQIXXXXXXXXXXXXXXXPLRQPRVI 61

Query: 66  SQMLVGLALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLSLIAKS 125
           ++++ G+ LGPS  G     L+A+FP KS  V ET A  G + F+FL G+++D   + ++
Sbjct: 62  AEVIGGIMLGPSLLGRSKAFLDAVFPKKSLTVLETLANLGLLFFLFLAGLEIDTKALRRT 121

Query: 126 GKKALVIGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVIACLLS 185
           GKKAL I +     P       +  LK+ +    N   FL  +G   + ++F V+A +L+
Sbjct: 122 GKKALGIALAGITLPFALGIGSSFVLKATISKGVNSTAFLVFMGVALSITAFPVLARILA 181

Query: 186 DLKLLNSDIGRLALSSSMISGTLSWIGLVASFTMRQTSMQQQDVLPWMALCVVCMIILVV 245
           +LKLL ++IGRLA+S++ ++   +WI L  +  +  ++     V  W+ L     +I   
Sbjct: 182 ELKLLTTEIGRLAMSAAAVNDVAAWILLALAIALSGSNTSPL-VSLWVFLSGCAFVIGAS 241

Query: 246 YILRPIMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILGLAVPD 305
           +I+ PI  WI  + +  G PI+ETY+ +   ++L C   ++ +G H + G  ++G+ +P 
Sbjct: 242 FIIPPIFRWISRRCH-EGEPIEETYICATLAVVLVCGFITDAIGIHSMFGAFVVGVLIPK 301

Query: 306 GPPLGSALVDKLDSFVSSIMLPCFFVISGARMNLSMFD-MRSASIIHLLAFTAFMGKLIG 365
             P   ALV+K++  VS + LP +FV SG + N++     +S  ++ L+  TA  GK++G
Sbjct: 302 EGPFAGALVEKVEDLVSGLFLPLYFVASGLKTNVATIQGAQSWGLLVLVTATACFGKILG 361

Query: 366 TMLPSLYCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMVMTGT 425
           T+  SL  K+ + ++++LG +M+ +G+ +++ L  G    +++  ++++ V+  +  T  
Sbjct: 362 TLGVSLAFKIPMREAITLGFLMNTKGLVELIVLNIGKDRKVLNDQTFAIMVLMALFTTFI 421

Query: 426 ICPIVKILYNPSKRYRCSA--RRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSNPT 485
             P+V  +Y P++R +     + R +E  + N +LR+L C H     PS+IN+LE S   
Sbjct: 422 TTPVVMAVYKPARRAKKEGEYKHRAVERENTNTQLRILTCFHGAGSIPSMINLLEASRGI 481

Query: 486 IKSP-ICFYLIHLLQLTGRASPLLIHHHHLRQRGSKHYKL------SDQIIKAFQIYQQF 545
            K   +C Y +HL +L+ R+S +L+  H +R+ G   +        +DQ++ AFQ +QQ 
Sbjct: 482 EKGEGLCVYALHLRELSERSSAILM-VHKVRKNGMPFWNRRGVNADADQVVVAFQAFQQL 541

Query: 546 NYDKVIMNAFTSVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESI-NPIRGVN 605
           +  +V +   T++S  + +H+D+C  A+ K+ A+VI+PFHK + ++G +E+     R VN
Sbjct: 542 S--RVNVRPMTAISSMSDIHEDICTTAVRKKAAIVILPFHKHQQLDGSLETTRGDYRWVN 601

Query: 606 KNILSKAPCSVGILIDREMLPSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAE 665
           + +L +APCSVGI +DR +  S+  S   ++    Y V ++F  GPDDREALAY  RMAE
Sbjct: 602 RRVLLQAPCSVGIFVDRGLGGSSQVSAQDVS----YSVVVLFFGGPDDREALAYGLRMAE 661

Query: 666 HPMVALTVVR-VIQPKR---------SSRHPADQDL-----DAEMINEFKLIMETSVLKH 725
           HP + LTV R V+ P+R         S+ +  +Q +     D E+++E + I  +SV + 
Sbjct: 662 HPGIVLTVFRFVVSPERVGEIVNVEVSNNNNENQSVKNLKSDEEIMSEIRKI--SSVDES 721

Query: 726 CTY-EEEMASDCVGLINVIRTMEHDYDMILVGRRHDGDSALFVGLHEWNEFPELGYIGDM 776
             + E+++ +  V + + I  +    ++ LVGR   G+ AL   + E +E PELG +G +
Sbjct: 722 VKFVEKQIENAAVDVRSAIEEVRRS-NLFLVGRMPGGEIAL--AIRENSECPELGPVGSL 781

BLAST of Bhi08G000351 vs. Swiss-Prot
Match: sp|Q9FFB8|CHX3_ARATH (Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 6.1e-101
Identity = 259/765 (33.86%), Postives = 424/765 (55.42%), Query Frame = 0

Query: 46  SLLTSLLQCLLTPLGESSFISQMLVGLALGPSFYGGDSPILE--AIFPFKSFYVSETFAY 105
           S L   L   L  LG   F S ML G+ L  SF   +S      +   +K    S T A 
Sbjct: 66  SFLWQFLHFFLRRLGMIRFTSHMLTGVLLSKSFLKENSAARRFFSTEDYKEIVFSLT-AA 125

Query: 106 FGCMVFMFLVGVKMDLSLIAKSGKKALVIGVLA-----FVTPMVFNFLLTTYLKSNVEMD 165
              M+F FL+GVKMD  LI  +G+KA+ IG+ +      V  ++F   L      N +  
Sbjct: 126 CSYMMFWFLMGVKMDTGLIRTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDVGTKNSDHT 185

Query: 166 ANLKNFLTTIGAFHASSSFHVIACLLSDLKLLNSDIGRLALSSSMISGTLSWIGLVASFT 225
            N   ++  I +    SSF V+  LL +L+L NS++GRLA+SS++IS   + I       
Sbjct: 186 LNSLEYV-VIYSIQCLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSILASVLIF 245

Query: 226 MRQTSMQQQ--------DVL----PWM-ALCVVCMIILVVYILRPIMIWIMEQTNLSGRP 285
           M++   +Q         DV+    P M A  VV  + + +Y+ RP+M +I++QT  SGRP
Sbjct: 246 MKELKDEQTRLGSVFIGDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQTP-SGRP 305

Query: 286 IKETYVLSLFLMLLFCALFSEFVGQHFILGPMILGLAVPDGPPLGSALVDKLDSFVSSIM 345
           +K  Y+ ++ +M+   A+ + +  Q   +GP ILGLAVP GPPLGSA++ K +S +    
Sbjct: 306 VKAIYLSTIIVMVSGSAILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQKYESAIFGTF 365

Query: 346 LPCFFVISGARMNLS-MFDMRSASIIHLLAFTAFMGKLIGTMLPSLYCKMSLVDSLSLGL 405
           LP F   S   +++S +F     + I L+  T+F+ K I T +P+L+  M + D  +L L
Sbjct: 366 LPFFIASSSTEIDISALFGWEGLNGIILIMVTSFVVKFIFTTVPALFYGMPMEDCFALSL 425

Query: 406 IMSAQGIADI----LGLQHGLLLYMIDQSSYSMTVVAMMVMTGTICPIVKILYNPSKRYR 465
           IMS +GI ++    L  Q G     +   ++++  + + + +  I PI++ LY+PS+ Y 
Sbjct: 426 IMSFKGIFELGAYALAYQRG----SVRPETFTVACLYITLNSAIIPPILRYLYDPSRMY- 485

Query: 466 CSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSNPTIKSPICFYLIHLLQLTGR 525
               +R ++H   N+ELR+L CI+  DD   +IN+LE   P+ +SP+  Y++HL++L G+
Sbjct: 486 AGYEKRNMQHLKPNSELRILSCIYRTDDISPMINLLEAICPSRESPVATYVLHLMELVGQ 545

Query: 526 ASPLLIHHHHLRQRGSKHYKLSDQIIKAFQIYQQFNYDKVIMNAFTSVSPYATMHDDVCM 585
           A+P+ I  H L+ R ++    S+ ++ +F+ +++  Y  V ++ +T++S   TMH D+CM
Sbjct: 546 ANPIFI-SHKLQTRRTEETSYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTMHGDICM 605

Query: 586 LALEKRVAMVIVPFHKRRTING--IVESINPIRGVNKNILSKAPCSVGILI-----DREM 645
           LAL    +++++PFH+  + +G  ++ + N IR +NK++L  APCSVG+ +      R+ 
Sbjct: 606 LALNNTTSLILLPFHQTWSADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRSSSGRKN 665

Query: 646 LPSTAASVS-LMNRVDLYKVGMIFVEGPDDREALAYATRMAEHPMVALTVVRVIQPKRSS 705
           + S   +++  +  +  Y + MIF+ G DDREA+  ATRMA  P + +T+VR+I     +
Sbjct: 666 ISSGRKTINGTVPNLSSYNICMIFLGGKDDREAVTLATRMARDPRINITIVRLITTDEKA 725

Query: 706 RHPA--DQDLDAEMINEFKLIMETSVLKHCTYEEEMASDCVGLINVIRTMEHDYDMILVG 765
           R     D+ LD E++ + K    ++ L    Y E+   D     +++R+M  D+DM +VG
Sbjct: 726 RENTVWDKMLDDELLRDVK----SNTLVDIFYSEKAIEDAAETSSLLRSMVSDFDMFIVG 785

Query: 766 RRHDGDSALFVGLHEWNEFPELGYIGDMLASSDSTGAVAVLVVQQ 776
           R +   S    GL EW+EF ELG IGD+L S D     +VLV+QQ
Sbjct: 786 RGNGRTSVFTEGLEEWSEFKELGIIGDLLTSQDFNCQASVLVIQQ 817

BLAST of Bhi08G000351 vs. TAIR10
Match: AT2G13620.1 (cation/hydrogen exchanger 15)

HSP 1 Score: 471.1 bits (1211), Expect = 1.4e-132
Identity = 289/798 (36.22%), Postives = 477/798 (59.77%), Query Frame = 0

Query: 2   STQAAHNGSWVCQPNRHYRSRGLFFGDSPFSFADSVLLAQLSLSSLLTSLLQCLLTPLGE 61
           S + + + S +C       + G++ GD+P  F+  + + QL+L  ++T     +L P  +
Sbjct: 4   SEEPSTDASIICYAPSMITTNGVWQGDNPLDFSLPLFVLQLTLVVVVTRFFVFILKPFRQ 63

Query: 62  SSFISQMLVGLALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLSL 121
              IS++L G+ LGPS  G  +     IFP +S  V ET A  G + F+FLVGV+MD+ +
Sbjct: 64  PRVISEILGGIVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLVGVEMDIMV 123

Query: 122 IAKSGKKALVIGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVIA 181
           + K+GK+AL I +   V P +      ++     E       ++  +G   + ++F V+A
Sbjct: 124 VRKTGKRALTIAIGGMVLPFLIGAAF-SFSMHRSEDHLGQGTYILFLGVALSVTAFPVLA 183

Query: 182 CLLSDLKLLNSDIGRLALSSSMISGTLSWIGLVASFTMRQTSMQQQDVLPWMALCVVCMI 241
            +L++LKL+N++IGR+++S+++++   +WI L  +  + ++       L W+ +     I
Sbjct: 184 RILAELKLINTEIGRISMSAALVNDMFAWILLALAIALAESDKTSFASL-WVMISSAVFI 243

Query: 242 ILVVYILRPIMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILGL 301
            + V+++RP + WI+ +T   G    E ++  +   ++     ++ +G H + G  + GL
Sbjct: 244 AVCVFVVRPGIAWIIRKTP-EGENFSEFHICLILTGVMISGFITDAIGTHSVFGAFVFGL 303

Query: 302 AVPDGPPLGSALVDKLDSFVSSIMLPCFFVISGARMNLSMFDMRSASI-IHLLAFTAFMG 361
            +P+G PLG  L++KL+ FVS ++LP FF ISG + N++     +  + + L+ F A  G
Sbjct: 304 VIPNG-PLGLTLIEKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLTLFLVIFLACAG 363

Query: 362 KLIGTMLPSLYCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMV 421
           K+IGT++ + +  M + + ++LGL+++ +G+ +++ L  G    ++D  +++  V+  +V
Sbjct: 364 KVIGTVIVAFFHGMPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFATMVLVALV 423

Query: 422 MTGTICPIVKILYNPSKRYRCSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSN 481
           MTG I PIV ILY P K+   S +RRTI+ T  ++ELR+L+C+H   + P+IIN+LE S+
Sbjct: 424 MTGVITPIVTILYKPVKK-SVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIINLLEASH 483

Query: 482 PTIKSPICFYLIHLLQLTGRASPLLIHHHHLRQRG----SKHYKLSDQIIKAFQIYQQFN 541
           PT +SPIC Y++HL++LTGRAS +LI  H+ R+ G    ++    SD II AF+ Y+Q +
Sbjct: 484 PTKRSPICIYVLHLVELTGRASAMLI-VHNTRKSGRPALNRTQAQSDHIINAFENYEQ-H 543

Query: 542 YDKVIMNAFTSVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESINP-IRGVNK 601
              V +   T++SPY+TMH+DVC LA +KRV+ +I+PFHK++T++G +ES NP  R VN+
Sbjct: 544 AAFVAVQPLTAISPYSTMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAYRLVNQ 603

Query: 602 NILSKAPCSVGILIDREMLPSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAEH 661
           N+L  +PCSVGIL+DR +  +T  +    N V L +V ++F  GPDDREALAYA RMA+H
Sbjct: 604 NLLENSPCSVGILVDRGLNGATRLN---SNTVSL-QVAVLFFGGPDDREALAYAWRMAQH 663

Query: 662 PMVALTVVRVIQPK-----RSSRHPADQD-------------LDAEMINEFKLIMETSVL 721
           P + LTV+R I  +      S+R   D D             LD + IN F+   E +  
Sbjct: 664 PGITLTVLRFIHDEDEADTASTRATNDSDLKIPKMDHRKQRQLDDDYINLFR--AENAEY 723

Query: 722 KHCTYEEEMASDCVGLINVIRTMEHDYDMILVGRRHDGDSALFVGLHEWNEFPELGYIGD 776
           +   Y E++ S+    +  +R+M+  +D+ +VGR     S L  GL +W+E PELG IGD
Sbjct: 724 ESIVYIEKLVSNGEETVAAVRSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGD 783

BLAST of Bhi08G000351 vs. TAIR10
Match: AT1G06970.1 (cation/hydrogen exchanger 14)

HSP 1 Score: 466.5 bits (1199), Expect = 3.5e-131
Identity = 268/788 (34.01%), Postives = 454/788 (57.61%), Query Frame = 0

Query: 12  VCQPNRHYRSRGLFFGDSPFSFADSVLLAQLSLSSLLTSLLQCLLTPLGESSFISQMLVG 71
           VCQ N    S+G+F G  P  +A  ++L Q+S+  + + LL  LL PL +    +Q+L G
Sbjct: 27  VCQKNHMLTSKGVFLGSDPLKYAMPLMLLQMSVIIITSRLLYRLLKPLKQGMISAQVLAG 86

Query: 72  LALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLSLIAKSGKKALV 131
           + LGPS +G  S  ++   P       +T +  G  + +FL+G+++D S+I K+G KA++
Sbjct: 87  IILGPSLFGQSSAYMQMFLPISGKITLQTLSNLGFFIHLFLLGLRIDASIIRKAGSKAIL 146

Query: 132 IGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVIACLLSDLKLLN 191
           IG  ++  P     L   +LK+   +  ++ + ++T+ + +A +SF V   +L++L +LN
Sbjct: 147 IGTASYALPFSLGNLTVLFLKNTYNLPPDVVHCISTVISLNAMTSFPVTTTVLAELNILN 206

Query: 192 SDIGRLALSSSMISGTLSWI-GLVASFTMRQTSMQQQDVLPWMALCVVCMIILVVYILRP 251
           SD+GRLA + S++    SWI  LV    +R  ++       W  + V  +I+++ ++ RP
Sbjct: 207 SDLGRLATNCSIVCEAFSWIVALVFRMFLRDGTLAS----VWSFVWVTALILVIFFVCRP 266

Query: 252 IMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILGLAVPDGPPLG 311
            +IW+ E+ ++S     E     + ++LL  +L SE +G H   G   LG+++PDGPPLG
Sbjct: 267 AIIWLTERRSISIDKAGEIPFFPIIMVLLTISLTSEVLGVHAAFGAFWLGVSLPDGPPLG 326

Query: 312 SALVDKLDSFVSSIMLPCFFVISGARMNLSMFDMRSASIIHLLAFTAFMGKLIGTMLPSL 371
           + L  KL+ F +S+MLPCF  ISG + N  +       II  +    +  K +GT   S 
Sbjct: 327 TGLTTKLEMFATSLMLPCFISISGLQTNFFIIGESHVKIIEAVILITYGCKFLGTAAASA 386

Query: 372 YCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMVMTGTICPIVK 431
           YC + + D+ SL L+M  QG+ +I          +++   +++ ++ ++++TG    +V 
Sbjct: 387 YCNIQIGDAFSLALLMCCQGVIEIYTCVMWKDEKVLNTECFNLLIITLLLVTGISRFLVV 446

Query: 432 ILYNPSKRYRCSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSNPTIKSPICFY 491
            LY+PSKRYR  ++R  ++    N + RLLLC+++ ++ PS++N+LE S P+  SPI  +
Sbjct: 447 CLYDPSKRYRSKSKRTILDTRQRNLQFRLLLCVYNVENVPSMVNLLEASYPSRFSPISVF 506

Query: 492 LIHLLQLTGRASPLLIHHHHLRQRGSKHYKLSDQIIKAFQIYQQFNYDKVIMNAFTSVSP 551
            +HL++L GRA  +L+ HH + +      + S  I+  FQ ++Q N   ++   FT+ +P
Sbjct: 507 TLHLVELKGRAHAVLVPHHQMNKLDPNTVQ-STHIVNGFQRFEQQNQGTLMAQHFTAAAP 566

Query: 552 YATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESINP-IRGVNKNILSKAPCSVGILI 611
           +++++DD+C LAL+K+  ++++PFHK+  I+G V+ +NP IR +N N+L KAPCSVGI I
Sbjct: 567 FSSINDDICTLALDKKATLIVIPFHKQYAIDGTVDHVNPSIRNINLNVLEKAPCSVGIFI 626

Query: 612 DREMLPSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAEHPMVALTVVRVIQPK 671
           DR     T    S++       V +IF+EG DD EALA++ R+AEHP V++T++   + K
Sbjct: 627 DR---GETEGRRSVLMSYTWRNVAVIFIEGRDDAEALAFSMRIAEHPEVSVTMIH-FRHK 686

Query: 672 RSSRHPADQDLDAE-----MINEFKLIMETSVLKHCTYEEEMASDCVGLINVIRTMEHDY 731
            S +     D+++E     +IN+FK    +      +Y EE+  D V    VI ++   +
Sbjct: 687 SSLQQNHVVDVESELAESYLINDFKNFAMSK--PKISYREEIVRDGVETTQVISSLGDSF 746

Query: 732 DMILVGRRHDGDSALFVGLHEWNEFPELGYIGDMLASSDSTGAVAVLVVQQADHRRRSGV 791
           D+++VGR HD +S++  GL +W+E PELG IGDM ASSD   +V V+  Q+ D       
Sbjct: 747 DLVVVGRDHDLESSVLYGLTDWSECPELGVIGDMFASSDFHFSVLVIHQQEGDSLAMDNS 803

Query: 792 FGRFPMPH 793
           +     PH
Sbjct: 807 YKLPASPH 803

BLAST of Bhi08G000351 vs. TAIR10
Match: AT2G30240.1 (Cation/hydrogen exchanger family protein)

HSP 1 Score: 404.8 bits (1039), Expect = 1.2e-112
Identity = 257/777 (33.08%), Postives = 429/777 (55.21%), Query Frame = 0

Query: 12  VCQPNRHYRSRGLFFGDSPFSFADSVLLAQLSLSSLLTSLLQCLLTPLGESSFISQMLVG 71
           VCQ      SRG+F   +P  +A  +LL Q+S+  + + L+  +L PL +    +Q+L G
Sbjct: 29  VCQAQNMLTSRGIFMKSNPLKYALPLLLLQMSVIIVTSRLIFRVLQPLKQGMISAQVLTG 88

Query: 72  LALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLSLIAKSGKKALV 131
           + LGPSF G +   +    P     + +T +  G ++ +FL+G+K+D S+I K+G KA++
Sbjct: 89  VVLGPSFLGHNVIYMNMFLPAGGKIIIQTLSNVGFVIHLFLLGLKIDGSIIRKAGSKAIL 148

Query: 132 IGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVIACLLSDLKLLN 191
           IG  ++  P     L   ++   + + +++ +  ++  +  + +SF V   +L++L +LN
Sbjct: 149 IGTASYAFPFSLGNLTIMFISKTMGLPSDVISCTSSAISLSSMTSFPVTTTVLAELNILN 208

Query: 192 SDIGRLALSSSMISGTLSW-IGLVASFTMRQTSMQQQDVLPWMALCVVCMIILVVY-ILR 251
           S++GRLA   SM+    SW + L  +   R  +M         AL ++  ++LV+Y + R
Sbjct: 209 SELGRLATHCSMVCEVCSWFVALAFNLYTRDRTMTS-----LYALSMIIGLLLVIYFVFR 268

Query: 252 PIMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILGLAVPDGPPL 311
           PI++W+ ++   S           + L+L   +L  E +G H   G   LG+++PDGPPL
Sbjct: 269 PIIVWLTQRKTKSMDKKDVVPFFPVLLLLSIASLSGEAMGVHAAFGAFWLGVSLPDGPPL 328

Query: 312 GSALVDKLDSFVSSIMLPCFFVISGARMN---LSMFDMRSASIIHLLAFTAFMGKLIGTM 371
           G+ L  KL+ F S++ LPCF  ISG + N   ++        +I ++    +  K +GT 
Sbjct: 329 GTELAAKLEMFASNLFLPCFIAISGLQTNFFEITESHEHHVVMIEIILLITYGCKFLGTA 388

Query: 372 LPSLYCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMVMTGTIC 431
             S YC+  + D+L L  +M  QGI ++          ++D   +++ ++ ++ +TG   
Sbjct: 389 AASAYCQTQIGDALCLAFLMCCQGIIEVYTTIVWKDAQVVDTECFNLVIITILFVTGISR 448

Query: 432 PIVKILYNPSKRYRCSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSNPTIKSP 491
            +V  LY+PSKRY+  ++R  +     N +LRLLL +++ ++ PS++N+LE + PT  +P
Sbjct: 449 FLVVYLYDPSKRYKSKSKRTILNTRQHNLQLRLLLGLYNVENVPSMVNLLEATYPTRFNP 508

Query: 492 ICFYLIHLLQLTGRASPLLIHHHHLRQRGSKHYKLSDQIIKAFQIYQQFNYDKVIMNAFT 551
           I F+ +HL++L GRA  LL  HH + +    +   S  I+ AFQ ++Q     ++   FT
Sbjct: 509 ISFFTLHLVELKGRAHALLTPHHQMNKL-DPNTAQSTHIVNAFQRFEQKYQGALMAQHFT 568

Query: 552 SVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESIN-PIRGVNKNILSKAPCSV 611
           + +PY+++++D+C LAL+K+  ++++PFHK+  I+G V  +N PIR +N N+L  APCSV
Sbjct: 569 AAAPYSSINNDICTLALDKKATLIVIPFHKQYAIDGTVGQVNGPIRTINLNVLDAAPCSV 628

Query: 612 GILIDREMLPSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAEHPMVALTVVRV 671
            I IDR     T    S++       V M+F+ G DD EALA   RMAE P + +T++  
Sbjct: 629 AIFIDR---GETEGRRSVLMTNTWQNVAMLFIGGKDDAEALALCMRMAEKPDLNVTMIHF 688

Query: 672 IQPKRSSRH-PADQDLDAEMINEFKLIME----TSVLKHCTYEEEMASDCVGLINVIRTM 731
                  RH  A QD D   ++E+ LI +     +      Y EE+  D V    VI ++
Sbjct: 689 -------RHKSALQDEDYSDMSEYNLISDFKSYAANKGKIHYVEEIVRDGVETTQVISSL 748

Query: 732 EHDYDMILVGRRHDGDSALFVGLHEWNEFPELGYIGDMLASSDSTGAVAVLVVQQAD 778
              YDM+LVGR HD +S++  GL +W+E PELG IGDML S D   +V V+  QQ D
Sbjct: 749 GDAYDMVLVGRDHDLESSVLYGLTDWSECPELGVIGDMLTSPDFHFSVLVVHQQQGD 789

BLAST of Bhi08G000351 vs. TAIR10
Match: AT5G41610.1 (cation/H+ exchanger 18)

HSP 1 Score: 377.9 bits (969), Expect = 1.6e-104
Identity = 252/797 (31.62%), Postives = 440/797 (55.21%), Query Frame = 0

Query: 6   AHNGSWVCQPNRHYRSRGLFFGDSPFSFADSVLLAQLSLSSLLTSLLQCLLTPLGESSFI 65
           A N +  C       S G+F GD+P  FA  + + Q+               PL +   I
Sbjct: 2   ATNSTKACPAPMKATSNGVFQGDNPIDFALPLAILQIXXXXXXXXXXXXXXXPLRQPRVI 61

Query: 66  SQMLVGLALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLSLIAKS 125
           ++++ G+ LGPS  G     L+A+FP KS  V ET A  G + F+FL G+++D   + ++
Sbjct: 62  AEVIGGIMLGPSLLGRSKAFLDAVFPKKSLTVLETLANLGLLFFLFLAGLEIDTKALRRT 121

Query: 126 GKKALVIGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVIACLLS 185
           GKKAL I +     P       +  LK+ +    N   FL  +G   + ++F V+A +L+
Sbjct: 122 GKKALGIALAGITLPFALGIGSSFVLKATISKGVNSTAFLVFMGVALSITAFPVLARILA 181

Query: 186 DLKLLNSDIGRLALSSSMISGTLSWIGLVASFTMRQTSMQQQDVLPWMALCVVCMIILVV 245
           +LKLL ++IGRLA+S++ ++   +WI L  +  +  ++     V  W+ L     +I   
Sbjct: 182 ELKLLTTEIGRLAMSAAAVNDVAAWILLALAIALSGSNTSPL-VSLWVFLSGCAFVIGAS 241

Query: 246 YILRPIMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILGLAVPD 305
           +I+ PI  WI  + +  G PI+ETY+ +   ++L C   ++ +G H + G  ++G+ +P 
Sbjct: 242 FIIPPIFRWISRRCH-EGEPIEETYICATLAVVLVCGFITDAIGIHSMFGAFVVGVLIPK 301

Query: 306 GPPLGSALVDKLDSFVSSIMLPCFFVISGARMNLSMFD-MRSASIIHLLAFTAFMGKLIG 365
             P   ALV+K++  VS + LP +FV SG + N++     +S  ++ L+  TA  GK++G
Sbjct: 302 EGPFAGALVEKVEDLVSGLFLPLYFVASGLKTNVATIQGAQSWGLLVLVTATACFGKILG 361

Query: 366 TMLPSLYCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMVMTGT 425
           T+  SL  K+ + ++++LG +M+ +G+ +++ L  G    +++  ++++ V+  +  T  
Sbjct: 362 TLGVSLAFKIPMREAITLGFLMNTKGLVELIVLNIGKDRKVLNDQTFAIMVLMALFTTFI 421

Query: 426 ICPIVKILYNPSKRYRCSA--RRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSNPT 485
             P+V  +Y P++R +     + R +E  + N +LR+L C H     PS+IN+LE S   
Sbjct: 422 TTPVVMAVYKPARRAKKEGEYKHRAVERENTNTQLRILTCFHGAGSIPSMINLLEASRGI 481

Query: 486 IKSP-ICFYLIHLLQLTGRASPLLIHHHHLRQRGSKHYKL------SDQIIKAFQIYQQF 545
            K   +C Y +HL +L+ R+S +L+  H +R+ G   +        +DQ++ AFQ +QQ 
Sbjct: 482 EKGEGLCVYALHLRELSERSSAILM-VHKVRKNGMPFWNRRGVNADADQVVVAFQAFQQL 541

Query: 546 NYDKVIMNAFTSVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESI-NPIRGVN 605
           +  +V +   T++S  + +H+D+C  A+ K+ A+VI+PFHK + ++G +E+     R VN
Sbjct: 542 S--RVNVRPMTAISSMSDIHEDICTTAVRKKAAIVILPFHKHQQLDGSLETTRGDYRWVN 601

Query: 606 KNILSKAPCSVGILIDREMLPSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAE 665
           + +L +APCSVGI +DR +  S+  S   ++    Y V ++F  GPDDREALAY  RMAE
Sbjct: 602 RRVLLQAPCSVGIFVDRGLGGSSQVSAQDVS----YSVVVLFFGGPDDREALAYGLRMAE 661

Query: 666 HPMVALTVVR-VIQPKR---------SSRHPADQDL-----DAEMINEFKLIMETSVLKH 725
           HP + LTV R V+ P+R         S+ +  +Q +     D E+++E + I  +SV + 
Sbjct: 662 HPGIVLTVFRFVVSPERVGEIVNVEVSNNNNENQSVKNLKSDEEIMSEIRKI--SSVDES 721

Query: 726 CTY-EEEMASDCVGLINVIRTMEHDYDMILVGRRHDGDSALFVGLHEWNEFPELGYIGDM 776
             + E+++ +  V + + I  +    ++ LVGR   G+ AL   + E +E PELG +G +
Sbjct: 722 VKFVEKQIENAAVDVRSAIEEVRRS-NLFLVGRMPGGEIAL--AIRENSECPELGPVGSL 781

BLAST of Bhi08G000351 vs. TAIR10
Match: AT5G22900.1 (cation/H+ exchanger 3)

HSP 1 Score: 370.2 bits (949), Expect = 3.4e-102
Identity = 259/765 (33.86%), Postives = 424/765 (55.42%), Query Frame = 0

Query: 46  SLLTSLLQCLLTPLGESSFISQMLVGLALGPSFYGGDSPILE--AIFPFKSFYVSETFAY 105
           S L   L   L  LG   F S ML G+ L  SF   +S      +   +K    S T A 
Sbjct: 66  SFLWQFLHFFLRRLGMIRFTSHMLTGVLLSKSFLKENSAARRFFSTEDYKEIVFSLT-AA 125

Query: 106 FGCMVFMFLVGVKMDLSLIAKSGKKALVIGVLA-----FVTPMVFNFLLTTYLKSNVEMD 165
              M+F FL+GVKMD  LI  +G+KA+ IG+ +      V  ++F   L      N +  
Sbjct: 126 CSYMMFWFLMGVKMDTGLIRTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDVGTKNSDHT 185

Query: 166 ANLKNFLTTIGAFHASSSFHVIACLLSDLKLLNSDIGRLALSSSMISGTLSWIGLVASFT 225
            N   ++  I +    SSF V+  LL +L+L NS++GRLA+SS++IS   + I       
Sbjct: 186 LNSLEYV-VIYSIQCLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSILASVLIF 245

Query: 226 MRQTSMQQQ--------DVL----PWM-ALCVVCMIILVVYILRPIMIWIMEQTNLSGRP 285
           M++   +Q         DV+    P M A  VV  + + +Y+ RP+M +I++QT  SGRP
Sbjct: 246 MKELKDEQTRLGSVFIGDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQTP-SGRP 305

Query: 286 IKETYVLSLFLMLLFCALFSEFVGQHFILGPMILGLAVPDGPPLGSALVDKLDSFVSSIM 345
           +K  Y+ ++ +M+   A+ + +  Q   +GP ILGLAVP GPPLGSA++ K +S +    
Sbjct: 306 VKAIYLSTIIVMVSGSAILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQKYESAIFGTF 365

Query: 346 LPCFFVISGARMNLS-MFDMRSASIIHLLAFTAFMGKLIGTMLPSLYCKMSLVDSLSLGL 405
           LP F   S   +++S +F     + I L+  T+F+ K I T +P+L+  M + D  +L L
Sbjct: 366 LPFFIASSSTEIDISALFGWEGLNGIILIMVTSFVVKFIFTTVPALFYGMPMEDCFALSL 425

Query: 406 IMSAQGIADI----LGLQHGLLLYMIDQSSYSMTVVAMMVMTGTICPIVKILYNPSKRYR 465
           IMS +GI ++    L  Q G     +   ++++  + + + +  I PI++ LY+PS+ Y 
Sbjct: 426 IMSFKGIFELGAYALAYQRG----SVRPETFTVACLYITLNSAIIPPILRYLYDPSRMY- 485

Query: 466 CSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSNPTIKSPICFYLIHLLQLTGR 525
               +R ++H   N+ELR+L CI+  DD   +IN+LE   P+ +SP+  Y++HL++L G+
Sbjct: 486 AGYEKRNMQHLKPNSELRILSCIYRTDDISPMINLLEAICPSRESPVATYVLHLMELVGQ 545

Query: 526 ASPLLIHHHHLRQRGSKHYKLSDQIIKAFQIYQQFNYDKVIMNAFTSVSPYATMHDDVCM 585
           A+P+ I  H L+ R ++    S+ ++ +F+ +++  Y  V ++ +T++S   TMH D+CM
Sbjct: 546 ANPIFI-SHKLQTRRTEETSYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTMHGDICM 605

Query: 586 LALEKRVAMVIVPFHKRRTING--IVESINPIRGVNKNILSKAPCSVGILI-----DREM 645
           LAL    +++++PFH+  + +G  ++ + N IR +NK++L  APCSVG+ +      R+ 
Sbjct: 606 LALNNTTSLILLPFHQTWSADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRSSSGRKN 665

Query: 646 LPSTAASVS-LMNRVDLYKVGMIFVEGPDDREALAYATRMAEHPMVALTVVRVIQPKRSS 705
           + S   +++  +  +  Y + MIF+ G DDREA+  ATRMA  P + +T+VR+I     +
Sbjct: 666 ISSGRKTINGTVPNLSSYNICMIFLGGKDDREAVTLATRMARDPRINITIVRLITTDEKA 725

Query: 706 RHPA--DQDLDAEMINEFKLIMETSVLKHCTYEEEMASDCVGLINVIRTMEHDYDMILVG 765
           R     D+ LD E++ + K    ++ L    Y E+   D     +++R+M  D+DM +VG
Sbjct: 726 RENTVWDKMLDDELLRDVK----SNTLVDIFYSEKAIEDAAETSSLLRSMVSDFDMFIVG 785

Query: 766 RRHDGDSALFVGLHEWNEFPELGYIGDMLASSDSTGAVAVLVVQQ 776
           R +   S    GL EW+EF ELG IGD+L S D     +VLV+QQ
Sbjct: 786 RGNGRTSVFTEGLEEWSEFKELGIIGDLLTSQDFNCQASVLVIQQ 817

BLAST of Bhi08G000351 vs. TrEMBL
Match: tr|A0A0A0LT09|A0A0A0LT09_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G025970 PE=4 SV=1)

HSP 1 Score: 1318.5 bits (3411), Expect = 0.0e+00
Identity = 682/775 (88.00%), Postives = 723/775 (93.29%), Query Frame = 0

Query: 1   MSTQAAHNGSWVCQPNRHYRSRGLFFGDSPFSFADSVLLAQLSLSSLLTSLLQCLLTPLG 60
           MSTQAAHNGSWVCQP  +YRSRG+FFGDSPFSFA ++LLAQLSLSS LTSLLQCLLTPLG
Sbjct: 1   MSTQAAHNGSWVCQPTTYYRSRGIFFGDSPFSFAKTILLAQLSLSSFLTSLLQCLLTPLG 60

Query: 61  ESSFISQMLVGLALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLS 120
           ESSFISQMLVGLALGPSFYGGD+PILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLS
Sbjct: 61  ESSFISQMLVGLALGPSFYGGDNPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLS 120

Query: 121 LIAKSGKKALVIGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVI 180
           LI KSGKKA+VIG +AF TPM+FNF L+TYLKS VEMDA+LKN L+ IGAF ASSSFHVI
Sbjct: 121 LIQKSGKKAMVIGFMAFSTPMLFNFFLSTYLKSTVEMDAHLKNTLSAIGAFQASSSFHVI 180

Query: 181 ACLLSDLKLLNSDIGRLALSSSMISGTLSWIGLVASFTMRQTSMQQQDVLPWMALCVVCM 240
           ACLL+DLKLLNSDIGRLALSSSMISGTL W GLV  FT+RQTSMQQQD LPWMALC+VCM
Sbjct: 181 ACLLTDLKLLNSDIGRLALSSSMISGTLCWGGLVIGFTLRQTSMQQQDALPWMALCLVCM 240

Query: 241 IILVVYILRPIMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILG 300
           +ILVVYILRPIM WI+EQTNLSGRPIKE YV  LFLMLLFC+LFSEFVGQHF+LGPMILG
Sbjct: 241 MILVVYILRPIMFWIIEQTNLSGRPIKEVYVFCLFLMLLFCSLFSEFVGQHFMLGPMILG 300

Query: 301 LAVPDGPPLGSALVDKLDSFVSSIMLPCFFVISGARMNLSMFDMRSASIIHLLAFTAFMG 360
           LAVPDGPPLGSALVDKLDSFVSSIMLPC+FVISGAR+NLS  ++RSA II+LLAFTAF+G
Sbjct: 301 LAVPDGPPLGSALVDKLDSFVSSIMLPCYFVISGARINLSTVNVRSAVIINLLAFTAFIG 360

Query: 361 KLIGTMLPSLYCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMV 420
           K+IGTMLPSLYCKMSLVDSLSLGLIMS QGIADILGLQHGLLLYMIDQ SYSM VVAMMV
Sbjct: 361 KVIGTMLPSLYCKMSLVDSLSLGLIMSTQGIADILGLQHGLLLYMIDQKSYSMMVVAMMV 420

Query: 421 MTGTICPIVKILYNPSKRYRCSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSN 480
           MTGTICPIVK++YNPSK+YRC  RRRTIEHTSA  ELRLLLCIHHQD+TPSIINMLELSN
Sbjct: 421 MTGTICPIVKMIYNPSKKYRCIMRRRTIEHTSATGELRLLLCIHHQDNTPSIINMLELSN 480

Query: 481 PTIKSPICFYLIHLLQLTGRASPLLIHHHHLRQRGSKHYKLSDQIIKAFQIYQQFNYDKV 540
           PTIKSPICFYLIHLLQLTGRASPLLI+HH   +RGSK   LSDQII AFQIYQQFNYDKV
Sbjct: 481 PTIKSPICFYLIHLLQLTGRASPLLINHHLPGRRGSKRCNLSDQIINAFQIYQQFNYDKV 540

Query: 541 IMNAFTSVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESINPIRGVNKNILSK 600
           IMNAFTSVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESIN IRGVNKNILSK
Sbjct: 541 IMNAFTSVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESINQIRGVNKNILSK 600

Query: 601 APCSVGILIDREMLPSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAEHPMVAL 660
           APCSVGILIDR + PS  +SVSL NRVDLYKVGMIFVEGPDDREALAYATRMAEHP VAL
Sbjct: 601 APCSVGILIDRVLSPSAVSSVSLTNRVDLYKVGMIFVEGPDDREALAYATRMAEHPKVAL 660

Query: 661 TVVRVIQPKRSSRHPADQDLDAEMINEFKLIMETSVLKHCTYEEEMASDCVGLINVIRTM 720
           TVVRVI+PKRSSRHP DQDLDAEMI EFKLIM TS  KH TYEEE+ASDCVGLINVIRTM
Sbjct: 661 TVVRVIEPKRSSRHPTDQDLDAEMIKEFKLIMATSENKHWTYEEEIASDCVGLINVIRTM 720

Query: 721 EHDYDMILVGRRHDGDSALFVGLHEWNEFPELGYIGDMLASSDSTGAVAVLVVQQ 776
           EHD D+ILVGRRHDGDSALFVGL+EWNE+PELG+IGDMLASSDS+GAVAVLV+QQ
Sbjct: 721 EHDSDLILVGRRHDGDSALFVGLNEWNEYPELGFIGDMLASSDSSGAVAVLVIQQ 775

BLAST of Bhi08G000351 vs. TrEMBL
Match: tr|A0A1S3C4R5|A0A1S3C4R5_CUCME (cation/H(+) antiporter 15-like OS=Cucumis melo OX=3656 GN=LOC103497021 PE=4 SV=1)

HSP 1 Score: 1201.8 bits (3108), Expect = 0.0e+00
Identity = 617/708 (87.15%), Postives = 663/708 (93.64%), Query Frame = 0

Query: 68  MLVGLALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLSLIAKSGK 127
           MLVGLALGPSFYGGD+PILEAIFPFKSFYVSETFAYFGCMVFMFLVG+KMDLSLI KSGK
Sbjct: 1   MLVGLALGPSFYGGDNPILEAIFPFKSFYVSETFAYFGCMVFMFLVGIKMDLSLIKKSGK 60

Query: 128 KALVIGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVIACLLSDL 187
           KA+VIG +AF+TPM+FNF L+TYLKS+VEMDA+LKN L  IGAF ASSSFHVIACLL+DL
Sbjct: 61  KAMVIGFMAFLTPMLFNFFLSTYLKSSVEMDAHLKNTLNAIGAFQASSSFHVIACLLTDL 120

Query: 188 KLLNSDIGRLALSSSMISGTLSWIGLVASFTMRQTSMQQQDVLPWMALCVVCMIILVVYI 247
           KLLNSDIGRLALSSSMISG LSW GLV  FT+RQTSMQQQD LPWMALC+VCM+ILV+YI
Sbjct: 121 KLLNSDIGRLALSSSMISGALSWAGLVIGFTLRQTSMQQQDALPWMALCLVCMMILVIYI 180

Query: 248 LRPIMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILGLAVPDGP 307
           LRPIM WI+EQTNLSGRPIKE YV  LFLMLLFCALFSEFVGQHF+LGPMILGLAVPDGP
Sbjct: 181 LRPIMFWIVEQTNLSGRPIKEVYVFCLFLMLLFCALFSEFVGQHFLLGPMILGLAVPDGP 240

Query: 308 PLGSALVDKLDSFVSSIMLPCFFVISGARMNLSMFDMRSASIIHLLAFTAFMGKLIGTML 367
           PLGSALVDKLDSFVSSIMLPC+FVISGAR+NLS  ++RSA II+LLAFTAF+GK+IGTML
Sbjct: 241 PLGSALVDKLDSFVSSIMLPCYFVISGARINLSTINVRSAVIINLLAFTAFIGKVIGTML 300

Query: 368 PSLYCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMVMTGTICP 427
           PSLYCKMSLVDSLSLGLIMS QGIADIL LQHGLLL+MIDQ+SYSM VVAMMVMTGTICP
Sbjct: 301 PSLYCKMSLVDSLSLGLIMSTQGIADILSLQHGLLLFMIDQTSYSMMVVAMMVMTGTICP 360

Query: 428 IVKILYNPSKRYRCSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSNPTIKSPI 487
           IVK++YNPSK+Y+C  RRRTIEHTSA  ELRLLLCIHHQD+TPSIINMLELSNPTIKSPI
Sbjct: 361 IVKMIYNPSKKYKCIMRRRTIEHTSATGELRLLLCIHHQDNTPSIINMLELSNPTIKSPI 420

Query: 488 CFYLIHLLQLTGRASPLLIHHHHLRQRGSKHYKLSDQIIKAFQIYQQFNYDKVIMNAFTS 547
           CFYLIHLLQLTGRASPLLI+HH   +RGSK   LSDQII AFQIYQQFNYDKVIMNAFTS
Sbjct: 421 CFYLIHLLQLTGRASPLLINHHLPGRRGSKRCNLSDQIINAFQIYQQFNYDKVIMNAFTS 480

Query: 548 VSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESINPIRGVNKNILSKAPCSVGI 607
           VSPYATMHDDVCMLALEKRVAMVIVPFH RRT NGIVESIN IRGVNKNILSKAPCSVGI
Sbjct: 481 VSPYATMHDDVCMLALEKRVAMVIVPFHNRRTFNGIVESINQIRGVNKNILSKAPCSVGI 540

Query: 608 LIDREMLPSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAEHPMVALTVVRVIQ 667
           LIDR MLPS  AS+SL NRVDLYKVGMIFVEGPDDREALAYATRMAEHP VALTVVRVI+
Sbjct: 541 LIDRVMLPSAVASISLTNRVDLYKVGMIFVEGPDDREALAYATRMAEHPKVALTVVRVIE 600

Query: 668 PKRSSRHPADQDLDAEMINEFKLIMETSVLKHCTYEEEMASDCVGLINVIRTMEHDYDMI 727
           PK+ +RHPADQDLDAEMI EFKLIM TS +KHC+YEEE+A+DCVGLINVIRTMEHDYD+I
Sbjct: 601 PKQ-TRHPADQDLDAEMIKEFKLIMATSGIKHCSYEEEIANDCVGLINVIRTMEHDYDLI 660

Query: 728 LVGRRHDGDSALFVGLHEWNEFPELGYIGDMLASSDSTGAVAVLVVQQ 776
           LVGRRHDGDSALFVGL+EWNE+PELG+IGDMLASSDS+GAVAVLV+QQ
Sbjct: 661 LVGRRHDGDSALFVGLNEWNEYPELGFIGDMLASSDSSGAVAVLVIQQ 707

BLAST of Bhi08G000351 vs. TrEMBL
Match: tr|A0A2P6QKG9|A0A2P6QKG9_ROSCH (Putative cation/H+ exchanger OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr5g0071771 PE=4 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 4.1e-235
Identity = 420/772 (54.40%), Postives = 576/772 (74.61%), Query Frame = 0

Query: 7   HNGSWVCQPNRHYRSRGLFFGDSPFSFADSVLLAQLSLSSLLTSLLQCLLTPLGESSFIS 66
           +  +W+C+  +  RS+G+F GD+P  +   +LL QL++++L+T+L Q +L PLGES++IS
Sbjct: 2   NRANWICETQKGIRSKGVFLGDNPLKYPSPILLLQLTIAALVTALFQFILNPLGESAYIS 61

Query: 67  QMLVGLALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLSLIAKSG 126
           QML G+ LGPSF GG++   E ++P KSFY+S+TFA+FGCM+F+FLVGVKMDLS++ +SG
Sbjct: 62  QMLAGILLGPSFLGGENDFAEIVYPVKSFYISQTFAFFGCMLFLFLVGVKMDLSIVRRSG 121

Query: 127 KKALVIGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVIACLLSD 186
           KKA+VIG+ AF  P+V N      L+  V M+  L   L  I  F   SSFHVI CLL D
Sbjct: 122 KKAVVIGLSAFFVPLVLNVGFALVLQRTVTMEPQLHKSLVAIAVFQCLSSFHVIGCLLGD 181

Query: 187 LKLLNSDIGRLALSSSMISGTLSWIGLVASFTMRQTSMQQQDVLPWMALCVVCMIILVVY 246
           LKLLNS++GRLA+SSSMISG +SWI ++ +FT+R ++M++   LP+M L +  + +L+++
Sbjct: 182 LKLLNSELGRLAVSSSMISGVISWIWVILAFTVRLSTMKKTGNLPFMGLSLAALFVLIIF 241

Query: 247 ILRPIMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILGLAVPDG 306
           I+RPIM+W++ QT  +G+PIKE+Y++S+F+M++ C+L  E VGQHF++GPM+LGLAVPDG
Sbjct: 242 IMRPIMLWMVAQT-ATGKPIKESYIVSIFIMVMICSLLGEIVGQHFLIGPMVLGLAVPDG 301

Query: 307 PPLGSALVDKLDSFVSSIMLPCFFVISGARMNLSMFDMRSASIIHLLAFTAFMGKLIGTM 366
           PPLGSALV+KLDS+VS+I+LP +F+ +GAR+NLS   M++  I+ +LA ++F GK++GTM
Sbjct: 302 PPLGSALVEKLDSYVSNILLPSYFLFTGARINLSEIRMKTVGIVEVLALSSFCGKVLGTM 361

Query: 367 LPSLYCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMVMTGTIC 426
           +PSLYCKM  +D+LSLGLIMSAQGI D+L LQHG+LL +ID   YS+ +++ ++ TG I 
Sbjct: 362 VPSLYCKMPAIDALSLGLIMSAQGITDLLVLQHGMLLLLIDGECYSIMILSSVLFTGIIT 421

Query: 427 PIVKILYNPSKRYRCSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSNPTIKSP 486
           PIVK LYNPSKRY+ + RRRTIEH+  N EL LL+C+ HQD TPSIIN++E+ NPT KSP
Sbjct: 422 PIVKFLYNPSKRYKSTKRRRTIEHSLPNMELGLLVCMFHQDSTPSIINLIEVFNPTPKSP 481

Query: 487 ICFYLIHLLQLTGRASPLLIHHHHLRQRGSKHYKLSDQIIKAFQIYQQFNYDKVIMNAFT 546
           ICF ++HL++L+GR +P+LI H   +         SD II AF++YQ+ N   VIMNAFT
Sbjct: 482 ICFCVVHLIRLSGRTAPVLITHRPGKNTSLHSNNYSDHIINAFRLYQEHNASSVIMNAFT 541

Query: 547 SVSPYATMHDDVCMLALEKRVAMVIVPFHK-RRTINGIVESINPIRGVNKNILSKAPCSV 606
           +++PYATMHD+VC LA EKR +M+I+PFHK    I    E  +PIR VN+NIL  APCSV
Sbjct: 542 AIAPYATMHDEVCTLAFEKRTSMIIIPFHKFFSAIQCTEELAHPIRSVNRNILQNAPCSV 601

Query: 607 GILIDREML--PSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAEHPMVALTVV 666
           GIL+DR  L    +A   S+  R  LY VG IFVEGPDDREALA ATRMA +P V+LTV 
Sbjct: 602 GILVDRGTLNKKPSALHTSISGR-SLYSVGTIFVEGPDDREALALATRMAGNPNVSLTVT 661

Query: 667 RVIQPKRSSRHPADQDLDAEMINEFKLIMETSVLKHCTYEEEMASDCVGLINVIRTMEHD 726
           +   PK++      +++D+E+I ++K  ++    K   Y+E    D V +IN IR+ME  
Sbjct: 662 KFTDPKQNK---TQKEIDSEVIMKYKTAIDGK--KQHVYKEVHVKDSVDMINEIRSMEDF 721

Query: 727 YDMILVGRRHDGDSALFVGLHEWNEFPELGYIGDMLASSDSTGAVAVLVVQQ 776
           +D+ILVGRRHD DS LF+GL EWNEFPELG++GDMLAS D+   V+VLVVQQ
Sbjct: 722 FDLILVGRRHDSDSPLFMGLTEWNEFPELGFLGDMLASFDANCEVSVLVVQQ 766

BLAST of Bhi08G000351 vs. TrEMBL
Match: tr|A0A2N9EI55|A0A2N9EI55_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS2212 PE=4 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 5.0e-233
Identity = 433/775 (55.87%), Postives = 572/775 (73.81%), Query Frame = 0

Query: 1   MSTQAAHNGSWVCQPNRHYRSRGLFFGDSPFSFADSVLLAQLSLSSLLTSLLQCLLTPLG 60
           M+T  A NG++ CQ  +   SRG+FFGD+PF FA  VL AQL+LS+LLTS LQ +LTPLG
Sbjct: 1   MTTSVAFNGTFFCQTRKDVTSRGVFFGDNPFDFAAPVLYAQLTLSALLTSFLQLILTPLG 60

Query: 61  ESSFISQMLVGLALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLS 120
           ES+FISQMLVG+ALGPSF G DS I   IF   SFY+++TFA+ GCM+FMFLVGVKMDL 
Sbjct: 61  ESAFISQMLVGIALGPSFLGKDSTIRHLIFTENSFYITKTFAFLGCMLFMFLVGVKMDLG 120

Query: 121 LIAKSGKKALVIGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVI 180
           ++ KSGKKA+VIG+ AF  P++ N      LKS + M+ +L   L +I  F +SSSFHVI
Sbjct: 121 MVKKSGKKAVVIGLCAFFLPLMLNTTFAFILKSTITMEHDLHESLISIAIFQSSSSFHVI 180

Query: 181 ACLLSDLKLLNSDIGRLALSSSMISGTLSWIGLVASFTMRQTSMQQQDVLPWMALCVVCM 240
           ACLL+DLKLLNS++GRLA+SSSM+SG  SW  L+ +F  RQ+S+ + + L  M   +  M
Sbjct: 181 ACLLADLKLLNSELGRLAMSSSMVSGICSWFWLIIAFMWRQSSINKHESLHLMVFGLGGM 240

Query: 241 IILVVYILRPIMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILG 300
           +  ++ ILRPI +W++ QT    + I E+Y+ S+F+M+L CALF E +GQHF+LGPMILG
Sbjct: 241 LFFIICILRPIFLWMVGQT-AKRKAITESYMFSVFVMVLMCALFGEVIGQHFMLGPMILG 300

Query: 301 LAVPDGPPLGSALVDKLDSFVSSIMLPCFFVISGARMNLSMFDMRSASIIHLLAFTAFMG 360
           LAVPDG PLGSALVDKLDS+VSSI+LP +FV S + ++L++  M++A I+ +LA  +  G
Sbjct: 301 LAVPDGWPLGSALVDKLDSYVSSILLPSYFVYSCSGIDLTLIHMKTAGIVEILALCSIFG 360

Query: 361 KLIGTMLPSLYCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMV 420
           K+ GTMLPSLYCKM ++DSLSLGLIMS  GI DIL LQHG LL+ IDQ S ++ ++++++
Sbjct: 361 KVTGTMLPSLYCKMPVIDSLSLGLIMSTHGITDILTLQHGRLLHFIDQKSLNILLISIVL 420

Query: 421 MTGTICPIVKILYNPSKRYRCSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSN 480
            TGTI PIVK+LY PSKRY    R RT++HT+ NAELRLL CI HQD TPS+IN+L+L N
Sbjct: 421 TTGTITPIVKLLYKPSKRYMSFRRWRTVQHTTPNAELRLLACIFHQDSTPSVINLLDLCN 480

Query: 481 PTIKSPICFYLIHLLQLTGRASPLLIHHHHLRQRGSKHYKLSDQIIKAFQIYQQFNYDKV 540
           P  K+PICFY++HL++L GR++PLL+ H    +R S        IIKAFQ+Y+Q N  K+
Sbjct: 481 PKPKTPICFYVVHLIKLLGRSAPLLVAHKQ-GKRNSSXXXXXXXIIKAFQLYEQHNRGKL 540

Query: 541 IMNAFTSVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESINPIRGVNKNILSK 600
           +++AFT+++PYA+MH ++C LALEKR +MVIVPFHK+ T++G+ ES NPIR VN  IL K
Sbjct: 541 MVHAFTAIAPYASMHHEICTLALEKRASMVIVPFHKQWTLHGVEESANPIRTVNFKILDK 600

Query: 601 APCSVGILIDREMLPSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAEHPMVAL 660
           APCSVGIL+D+  L   A   S      LY V +IFV GPDDREAL+ A RM EHP V L
Sbjct: 601 APCSVGILVDKGTLNGGAPGRS----KPLYCVAVIFVGGPDDREALSCAMRMVEHPNVGL 660

Query: 661 TVVRVIQPKRSSRHPADQDLDAEMINEFKLIMETSVLKHCTYEEEMASDCVGLINVIRTM 720
           TV+++++   + ++   + LD +MIN+FK  M     KH ++ EE+  D V +INVIR++
Sbjct: 661 TVIQLVESNGNGKNLVSKKLDCDMINQFK--MANIQKKHHSHREEVVKDSVEMINVIRSL 720

Query: 721 EHDYDMILVGRRHDGDSALFVGLHEWNEFPELGYIGDMLASSDSTGAVAVLVVQQ 776
           E+ YD+ILVGRRHD +S LF GL  WN+FPELG++GDML SS S    +V VVQQ
Sbjct: 721 ENLYDLILVGRRHDSNSPLFGGLTLWNDFPELGFLGDMLVSSGSNWYESVFVVQQ 767

BLAST of Bhi08G000351 vs. TrEMBL
Match: tr|A0A251NM28|A0A251NM28_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G047700 PE=4 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 4.8e-228
Identity = 429/780 (55.00%), Postives = 574/780 (73.59%), Query Frame = 0

Query: 3   TQAAHNGSWVCQPNRHYRSRGLFFGDSPFSFADSVLLAQLSLSSLLTSLLQCLLTPLGES 62
           TQA  NG+WVCQ  +  RS+G FFGD+P  F  ++L  QLS+S+LLTS LQ +L PLGES
Sbjct: 5   TQALSNGTWVCQAQKGSRSKGFFFGDNPLDFPTTILYVQLSISALLTSFLQFILNPLGES 64

Query: 63  SFISQMLVGLALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLSLI 122
           +F SQMLVG+ LGPS  GG +   + +FP KSFY+SETFA+FG M+F+FLVGVKMD+S++
Sbjct: 65  TFTSQMLVGIILGPSVLGGGNVFADTVFPMKSFYISETFAFFGVMLFLFLVGVKMDISVV 124

Query: 123 AKSGKKALVIGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVIAC 182
            ++G+KALVIG+ AF  P++ N      L+  V M+  L   +  I  F + SSFHVIAC
Sbjct: 125 KQTGRKALVIGISAFFVPLILNMGFALVLQRTVTMEPKLHKSIIIIAVFQSMSSFHVIAC 184

Query: 183 LLSDLKLLNSDIGRLALSSSMISGTLSWIGLVASFTMRQTSM-QQQDVLPWMALCVVCMI 242
           LL+DLKLLNS+IGRLA+SSSM+SG LS+  L  +F  RQ+S+ + +  LP+M + V CM 
Sbjct: 185 LLADLKLLNSEIGRLAVSSSMVSGVLSFACLSLAFATRQSSIGRNKFSLPFMGISVFCMF 244

Query: 243 ILVVYILRPIMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILGL 302
           IL VYI+RPIM+W++ QT+  G+P K+ Y+  +F+M++ C+L  E +G HF+LGPMILGL
Sbjct: 245 ILTVYIMRPIMLWMVAQTH-KGKPFKDGYICYIFIMVMSCSLIGEVMGLHFMLGPMILGL 304

Query: 303 AVPDGPPLGSALVDKLDSFVSSIMLPCFFVISGARMNLSMFDMRSASIIHLLAFTAFMGK 362
           AVP+GPPLGSALV+KLDS++S I+LP +FV   AR+N S+  M++  ++ LL  + F GK
Sbjct: 305 AVPEGPPLGSALVEKLDSYISLILLPSYFVFGAARINFSLIKMKTVWVVELLTVSGFCGK 364

Query: 363 LIGTMLPSLYCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMVM 422
           LIGT++PSLYCKM  VD+ SLGLIMSAQGI D++ LQHGL L +ID+ S+S+  ++ +V 
Sbjct: 365 LIGTVVPSLYCKMPAVDACSLGLIMSAQGIIDVITLQHGLFLKLIDEESFSIMALSALVS 424

Query: 423 TGTICPIVKILYNPSKRYRCSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSNP 482
           T  I P+VK LY+PSKRY  + RRRTIEH   NAELR+L CI+ +D TP IIN+LE+SNP
Sbjct: 425 TAIITPVVKFLYDPSKRYMSTMRRRTIEHALPNAELRMLACIYQEDSTPPIINLLEVSNP 484

Query: 483 TIKSPICFYLIHLLQLTGRASPLLIHHHHLRQRGSKHYKL--SDQIIKAFQIYQQFNYDK 542
           T K+PI FY++HL++L+GR +P+LI H    +R ++ ++   SD+I+ AF++Y++ +   
Sbjct: 485 TPKTPIFFYVVHLIRLSGRTAPVLITHRP-GKRSARTFESHDSDRIVNAFRLYEEHSSGG 544

Query: 543 VIMNAFTSVSPYATMHDDVCMLALEKRVAMVIVPFHKR-RTINGI-VESINPIRGVNKNI 602
           +IMNA T++SPYATMHDDVC LALEKR +MV++PFHK+   ++G  V   NPIR VN+ I
Sbjct: 545 LIMNALTAISPYATMHDDVCTLALEKRTSMVLIPFHKQFNELSGAGVLDNNPIRSVNQKI 604

Query: 603 LSKAPCSVGILIDREMLPSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAEHPM 662
           L  +PCSVGIL+DR  L +T  ++S  N    Y  GMIFVEGPDDREALAYA RMAEHP 
Sbjct: 605 LRNSPCSVGILLDRGTL-NTNVTISCKNS---YNFGMIFVEGPDDREALAYAMRMAEHPN 664

Query: 663 VALTVVRVIQPKRSSRHPADQDLDAEMINEFKLIMETSVLKHCTYEEEMASDCVGLINVI 722
           V+LTV+R++    + +H   +D D  +I++ K  +  S  K   Y+EE   D V +INVI
Sbjct: 665 VSLTVIRLVD--HNMKHSVHRDHDMVLISKCKTAI--SGRKQHVYKEEHVKDSVDMINVI 724

Query: 723 R--TMEHDYDMILVGRRHDGDSALFVGLHEWNEFPELGYIGDMLASSDSTGAVAVLVVQQ 776
           R  +ME  YD+ILVG RHD +S LF+GL EWNEFPELG+IGDMLASSDS   V+VLVVQQ
Sbjct: 725 RSNSMEKLYDLILVGGRHDSNSPLFMGLTEWNEFPELGFIGDMLASSDSNCDVSVLVVQQ 774

BLAST of Bhi08G000351 vs. NCBI nr
Match: XP_004137561.1 (PREDICTED: cation/H(+) antiporter 15-like [Cucumis sativus] >KGN63917.1 hypothetical protein Csa_1G025970 [Cucumis sativus])

HSP 1 Score: 1318.5 bits (3411), Expect = 0.0e+00
Identity = 682/775 (88.00%), Postives = 723/775 (93.29%), Query Frame = 0

Query: 1   MSTQAAHNGSWVCQPNRHYRSRGLFFGDSPFSFADSVLLAQLSLSSLLTSLLQCLLTPLG 60
           MSTQAAHNGSWVCQP  +YRSRG+FFGDSPFSFA ++LLAQLSLSS LTSLLQCLLTPLG
Sbjct: 1   MSTQAAHNGSWVCQPTTYYRSRGIFFGDSPFSFAKTILLAQLSLSSFLTSLLQCLLTPLG 60

Query: 61  ESSFISQMLVGLALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLS 120
           ESSFISQMLVGLALGPSFYGGD+PILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLS
Sbjct: 61  ESSFISQMLVGLALGPSFYGGDNPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLS 120

Query: 121 LIAKSGKKALVIGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVI 180
           LI KSGKKA+VIG +AF TPM+FNF L+TYLKS VEMDA+LKN L+ IGAF ASSSFHVI
Sbjct: 121 LIQKSGKKAMVIGFMAFSTPMLFNFFLSTYLKSTVEMDAHLKNTLSAIGAFQASSSFHVI 180

Query: 181 ACLLSDLKLLNSDIGRLALSSSMISGTLSWIGLVASFTMRQTSMQQQDVLPWMALCVVCM 240
           ACLL+DLKLLNSDIGRLALSSSMISGTL W GLV  FT+RQTSMQQQD LPWMALC+VCM
Sbjct: 181 ACLLTDLKLLNSDIGRLALSSSMISGTLCWGGLVIGFTLRQTSMQQQDALPWMALCLVCM 240

Query: 241 IILVVYILRPIMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILG 300
           +ILVVYILRPIM WI+EQTNLSGRPIKE YV  LFLMLLFC+LFSEFVGQHF+LGPMILG
Sbjct: 241 MILVVYILRPIMFWIIEQTNLSGRPIKEVYVFCLFLMLLFCSLFSEFVGQHFMLGPMILG 300

Query: 301 LAVPDGPPLGSALVDKLDSFVSSIMLPCFFVISGARMNLSMFDMRSASIIHLLAFTAFMG 360
           LAVPDGPPLGSALVDKLDSFVSSIMLPC+FVISGAR+NLS  ++RSA II+LLAFTAF+G
Sbjct: 301 LAVPDGPPLGSALVDKLDSFVSSIMLPCYFVISGARINLSTVNVRSAVIINLLAFTAFIG 360

Query: 361 KLIGTMLPSLYCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMV 420
           K+IGTMLPSLYCKMSLVDSLSLGLIMS QGIADILGLQHGLLLYMIDQ SYSM VVAMMV
Sbjct: 361 KVIGTMLPSLYCKMSLVDSLSLGLIMSTQGIADILGLQHGLLLYMIDQKSYSMMVVAMMV 420

Query: 421 MTGTICPIVKILYNPSKRYRCSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSN 480
           MTGTICPIVK++YNPSK+YRC  RRRTIEHTSA  ELRLLLCIHHQD+TPSIINMLELSN
Sbjct: 421 MTGTICPIVKMIYNPSKKYRCIMRRRTIEHTSATGELRLLLCIHHQDNTPSIINMLELSN 480

Query: 481 PTIKSPICFYLIHLLQLTGRASPLLIHHHHLRQRGSKHYKLSDQIIKAFQIYQQFNYDKV 540
           PTIKSPICFYLIHLLQLTGRASPLLI+HH   +RGSK   LSDQII AFQIYQQFNYDKV
Sbjct: 481 PTIKSPICFYLIHLLQLTGRASPLLINHHLPGRRGSKRCNLSDQIINAFQIYQQFNYDKV 540

Query: 541 IMNAFTSVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESINPIRGVNKNILSK 600
           IMNAFTSVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESIN IRGVNKNILSK
Sbjct: 541 IMNAFTSVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESINQIRGVNKNILSK 600

Query: 601 APCSVGILIDREMLPSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAEHPMVAL 660
           APCSVGILIDR + PS  +SVSL NRVDLYKVGMIFVEGPDDREALAYATRMAEHP VAL
Sbjct: 601 APCSVGILIDRVLSPSAVSSVSLTNRVDLYKVGMIFVEGPDDREALAYATRMAEHPKVAL 660

Query: 661 TVVRVIQPKRSSRHPADQDLDAEMINEFKLIMETSVLKHCTYEEEMASDCVGLINVIRTM 720
           TVVRVI+PKRSSRHP DQDLDAEMI EFKLIM TS  KH TYEEE+ASDCVGLINVIRTM
Sbjct: 661 TVVRVIEPKRSSRHPTDQDLDAEMIKEFKLIMATSENKHWTYEEEIASDCVGLINVIRTM 720

Query: 721 EHDYDMILVGRRHDGDSALFVGLHEWNEFPELGYIGDMLASSDSTGAVAVLVVQQ 776
           EHD D+ILVGRRHDGDSALFVGL+EWNE+PELG+IGDMLASSDS+GAVAVLV+QQ
Sbjct: 721 EHDSDLILVGRRHDGDSALFVGLNEWNEYPELGFIGDMLASSDSSGAVAVLVIQQ 775

BLAST of Bhi08G000351 vs. NCBI nr
Match: XP_008457299.2 (PREDICTED: cation/H(+) antiporter 15-like [Cucumis melo])

HSP 1 Score: 1201.8 bits (3108), Expect = 0.0e+00
Identity = 617/708 (87.15%), Postives = 663/708 (93.64%), Query Frame = 0

Query: 68  MLVGLALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLSLIAKSGK 127
           MLVGLALGPSFYGGD+PILEAIFPFKSFYVSETFAYFGCMVFMFLVG+KMDLSLI KSGK
Sbjct: 1   MLVGLALGPSFYGGDNPILEAIFPFKSFYVSETFAYFGCMVFMFLVGIKMDLSLIKKSGK 60

Query: 128 KALVIGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVIACLLSDL 187
           KA+VIG +AF+TPM+FNF L+TYLKS+VEMDA+LKN L  IGAF ASSSFHVIACLL+DL
Sbjct: 61  KAMVIGFMAFLTPMLFNFFLSTYLKSSVEMDAHLKNTLNAIGAFQASSSFHVIACLLTDL 120

Query: 188 KLLNSDIGRLALSSSMISGTLSWIGLVASFTMRQTSMQQQDVLPWMALCVVCMIILVVYI 247
           KLLNSDIGRLALSSSMISG LSW GLV  FT+RQTSMQQQD LPWMALC+VCM+ILV+YI
Sbjct: 121 KLLNSDIGRLALSSSMISGALSWAGLVIGFTLRQTSMQQQDALPWMALCLVCMMILVIYI 180

Query: 248 LRPIMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILGLAVPDGP 307
           LRPIM WI+EQTNLSGRPIKE YV  LFLMLLFCALFSEFVGQHF+LGPMILGLAVPDGP
Sbjct: 181 LRPIMFWIVEQTNLSGRPIKEVYVFCLFLMLLFCALFSEFVGQHFLLGPMILGLAVPDGP 240

Query: 308 PLGSALVDKLDSFVSSIMLPCFFVISGARMNLSMFDMRSASIIHLLAFTAFMGKLIGTML 367
           PLGSALVDKLDSFVSSIMLPC+FVISGAR+NLS  ++RSA II+LLAFTAF+GK+IGTML
Sbjct: 241 PLGSALVDKLDSFVSSIMLPCYFVISGARINLSTINVRSAVIINLLAFTAFIGKVIGTML 300

Query: 368 PSLYCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMVMTGTICP 427
           PSLYCKMSLVDSLSLGLIMS QGIADIL LQHGLLL+MIDQ+SYSM VVAMMVMTGTICP
Sbjct: 301 PSLYCKMSLVDSLSLGLIMSTQGIADILSLQHGLLLFMIDQTSYSMMVVAMMVMTGTICP 360

Query: 428 IVKILYNPSKRYRCSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSNPTIKSPI 487
           IVK++YNPSK+Y+C  RRRTIEHTSA  ELRLLLCIHHQD+TPSIINMLELSNPTIKSPI
Sbjct: 361 IVKMIYNPSKKYKCIMRRRTIEHTSATGELRLLLCIHHQDNTPSIINMLELSNPTIKSPI 420

Query: 488 CFYLIHLLQLTGRASPLLIHHHHLRQRGSKHYKLSDQIIKAFQIYQQFNYDKVIMNAFTS 547
           CFYLIHLLQLTGRASPLLI+HH   +RGSK   LSDQII AFQIYQQFNYDKVIMNAFTS
Sbjct: 421 CFYLIHLLQLTGRASPLLINHHLPGRRGSKRCNLSDQIINAFQIYQQFNYDKVIMNAFTS 480

Query: 548 VSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESINPIRGVNKNILSKAPCSVGI 607
           VSPYATMHDDVCMLALEKRVAMVIVPFH RRT NGIVESIN IRGVNKNILSKAPCSVGI
Sbjct: 481 VSPYATMHDDVCMLALEKRVAMVIVPFHNRRTFNGIVESINQIRGVNKNILSKAPCSVGI 540

Query: 608 LIDREMLPSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAEHPMVALTVVRVIQ 667
           LIDR MLPS  AS+SL NRVDLYKVGMIFVEGPDDREALAYATRMAEHP VALTVVRVI+
Sbjct: 541 LIDRVMLPSAVASISLTNRVDLYKVGMIFVEGPDDREALAYATRMAEHPKVALTVVRVIE 600

Query: 668 PKRSSRHPADQDLDAEMINEFKLIMETSVLKHCTYEEEMASDCVGLINVIRTMEHDYDMI 727
           PK+ +RHPADQDLDAEMI EFKLIM TS +KHC+YEEE+A+DCVGLINVIRTMEHDYD+I
Sbjct: 601 PKQ-TRHPADQDLDAEMIKEFKLIMATSGIKHCSYEEEIANDCVGLINVIRTMEHDYDLI 660

Query: 728 LVGRRHDGDSALFVGLHEWNEFPELGYIGDMLASSDSTGAVAVLVVQQ 776
           LVGRRHDGDSALFVGL+EWNE+PELG+IGDMLASSDS+GAVAVLV+QQ
Sbjct: 661 LVGRRHDGDSALFVGLNEWNEYPELGFIGDMLASSDSSGAVAVLVIQQ 707

BLAST of Bhi08G000351 vs. NCBI nr
Match: XP_022970379.1 (cation/H(+) antiporter 15-like [Cucurbita maxima])

HSP 1 Score: 1156.4 bits (2990), Expect = 0.0e+00
Identity = 584/775 (75.35%), Postives = 678/775 (87.48%), Query Frame = 0

Query: 1   MSTQAAHNGSWVCQPNRHYRSRGLFFGDSPFSFADSVLLAQLSLSSLLTSLLQCLLTPLG 60
           MS +AAHNGSWVCQPN  YRSRG+FFGD+PFSF ++V LAQ+S+SSLLT LLQCLLTP+G
Sbjct: 1   MSIRAAHNGSWVCQPNERYRSRGIFFGDNPFSFGNTVFLAQISVSSLLTCLLQCLLTPIG 60

Query: 61  ESSFISQMLVGLALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLS 120
           ESSF SQMLVGLALGPSF GG+SP+LE +FP+KSFYVSETFA+FGCM+FMFL+GVKMDL+
Sbjct: 61  ESSFFSQMLVGLALGPSFNGGNSPLLEVVFPYKSFYVSETFAFFGCMIFMFLMGVKMDLT 120

Query: 121 LIAKSGKKALVIGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVI 180
           LI KSG KA+VIGVL F+ P++ N+LL+ YLKS ++MD NLK+ LT IGAF +SSSF+VI
Sbjct: 121 LITKSGTKAMVIGVLVFLFPLLINYLLSVYLKSTIDMDGNLKDSLTAIGAFQSSSSFYVI 180

Query: 181 ACLLSDLKLLNSDIGRLALSSSMISGTLSWIGLVASFTMRQTSMQQQDVLPWMALCVVCM 240
           AC+L DL LLNS+IGRLALSSSMISGTLSWI +V  FT+RQTS++QQD LPWMA+CV CM
Sbjct: 181 ACVLEDLNLLNSNIGRLALSSSMISGTLSWISIVICFTLRQTSLEQQDALPWMAVCVACM 240

Query: 241 IILVVYILRPIMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILG 300
           IILV+YILRPIM WI+EQTN+SGRPIKE+YV  +F+M+LFC+LFSEFVGQHF+LGPMILG
Sbjct: 241 IILVIYILRPIMFWIVEQTNISGRPIKESYVFLMFIMMLFCSLFSEFVGQHFLLGPMILG 300

Query: 301 LAVPDGPPLGSALVDKLDSFVSSIMLPCFFVISGARMNLSMFDMRSASIIHLLAFTAFMG 360
           LAVPDGPPLGSALVDKL+SFVSSIMLPC+FVISGAR+NLS  DM+S  I+ LLAF + MG
Sbjct: 301 LAVPDGPPLGSALVDKLESFVSSIMLPCYFVISGARINLSKLDMKSIWIVQLLAFGSLMG 360

Query: 361 KLIGTMLPSLYCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMV 420
           KLIG  LPSLYCKM LVDSL+LGLIMS QGI+DIL LQ GLLL M+D ++YS+ V+AMMV
Sbjct: 361 KLIGATLPSLYCKMPLVDSLTLGLIMSTQGISDILFLQQGLLLQMLDVNAYSVMVLAMMV 420

Query: 421 MTGTICPIVKILYNPSKRYRCSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSN 480
           MTG   PIVK+LYNPS +YRC+ RRRTIEHTS N ELR+LLCIHHQD+TPSIIN+LE+SN
Sbjct: 421 MTGATSPIVKMLYNPSNKYRCNRRRRTIEHTSPNGELRVLLCIHHQDNTPSIINLLEVSN 480

Query: 481 PTIKSPICFYLIHLLQLTGRASPLLIHHHHLRQRGSKHYKLSDQIIKAFQIYQQFNYDKV 540
           PTIKSPICFYLIHL+QLTGRASPLLIHHH  R R SK   LSDQII AFQ++QQ +YDKV
Sbjct: 481 PTIKSPICFYLIHLIQLTGRASPLLIHHHPSR-RSSKRCNLSDQIINAFQLFQQMSYDKV 540

Query: 541 IMNAFTSVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESINPIRGVNKNILSK 600
           IMNAFTSV+P+ATMHDDVCMLALEKRV+MVIVPFHKRRT+N    S+N IR VNKNILSK
Sbjct: 541 IMNAFTSVAPFATMHDDVCMLALEKRVSMVIVPFHKRRTMNVYEGSVNAIRAVNKNILSK 600

Query: 601 APCSVGILIDREMLPSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAEHPMVAL 660
           APCSVGILID  +LP+TA +V +MNRV+LYKVG+IFV G DDREALAYATRM EHPMV L
Sbjct: 601 APCSVGILIDPMILPTTANTVPIMNRVELYKVGLIFVGGSDDREALAYATRMVEHPMVTL 660

Query: 661 TVVRVIQPKRSSRHPADQDLDAEMINEFKLIMETSVLKHCTYEEEMASDCVGLINVIRTM 720
           TVVR+I PK ++  PADQ+ D EM+NEFKLIM +S +KHC YEE  ASDCVGLI V+R M
Sbjct: 661 TVVRLIHPKGTTHQPADQEHDFEMLNEFKLIMSSSEIKHCFYEEVTASDCVGLIGVMRKM 720

Query: 721 EHDYDMILVGRRHDGDSALFVGLHEWNEFPELGYIGDMLASSDSTGAVAVLVVQQ 776
           E +YD+ILVGRRHDGDS LFVGL+EW+E+PELG+IGDMLA+ DS     VLV+QQ
Sbjct: 721 ESNYDLILVGRRHDGDSELFVGLNEWHEYPELGFIGDMLATIDSPSDATVLVIQQ 774

BLAST of Bhi08G000351 vs. NCBI nr
Match: XP_022949065.1 (cation/H(+) antiporter 15-like [Cucurbita moschata])

HSP 1 Score: 1150.6 bits (2975), Expect = 0.0e+00
Identity = 587/776 (75.64%), Postives = 686/776 (88.40%), Query Frame = 0

Query: 1   MSTQAAHNGSWVCQPNRHYRSRGLFFGDSPFSFADSVLLAQLSLSSLLTSLLQCLLTPLG 60
           MSTQAA NGSWVCQP+ +Y+SRG+FFGD+PFS+  S+LLAQLSLSSLLTSLLQC+LTPLG
Sbjct: 1   MSTQAAQNGSWVCQPSSNYQSRGIFFGDTPFSYGSSILLAQLSLSSLLTSLLQCILTPLG 60

Query: 61  ESSFISQMLVGLALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLS 120
           ESSFISQMLVGL LGPSFYGGDSP+LEAIFPFKSFYVSETFA+FGC+VFMFLVGVKMDL+
Sbjct: 61  ESSFISQMLVGLMLGPSFYGGDSPVLEAIFPFKSFYVSETFAFFGCLVFMFLVGVKMDLT 120

Query: 121 LIAKSGKKALVIGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVI 180
           +I ++G+KA+VIGVLAF++P+  NF+L +YLK++++MD  LKN LT IGAF ASSSFHVI
Sbjct: 121 IIQRTGRKAMVIGVLAFISPLFINFILASYLKNSIDMDLQLKNSLTAIGAFQASSSFHVI 180

Query: 181 ACLLSDLKLLNSDIGRLALSSSMISGTLSWIGLVASFTMRQTSMQQQDVLPWMALCVVCM 240
           AC L+D+ LLNSDIGRLALSSSMISG LSW  +V  FT++QTS +QQD LPWMALC+VCM
Sbjct: 181 ACYLADINLLNSDIGRLALSSSMISGMLSWFAIVVVFTVQQTSNRQQDALPWMALCIVCM 240

Query: 241 IILVVYILRPIMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILG 300
           IILV+YILRPIM+WI+EQTN SGR IKE YVL +FLMLLFCALFSEFVGQHF+LGPMILG
Sbjct: 241 IILVIYILRPIMLWIIEQTNNSGRVIKEGYVLLVFLMLLFCALFSEFVGQHFMLGPMILG 300

Query: 301 LAVPDGPPLGSALVDKLDSFVSSIMLPCFFVISGARMNLSMFDMRSASIIHLLAFTAFMG 360
           LAVPDGPPLG+ALVDKLDSFVSS+MLPC+FVISGAR+NLS  +MRSA I+ +LA  +F+G
Sbjct: 301 LAVPDGPPLGTALVDKLDSFVSSVMLPCYFVISGARINLSEINMRSAWIVQVLALGSFLG 360

Query: 361 KLIGTMLPSLYCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMV 420
           KLIGT+LPSLYCKM LVDSLSLGLIMS QGIADIL LQH +LL+MIDQ SY+M VVAMMV
Sbjct: 361 KLIGTVLPSLYCKMPLVDSLSLGLIMSTQGIADILVLQHAMLLFMIDQKSYAMMVVAMMV 420

Query: 421 MTGTICPIVKILYNPSKRYRCSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSN 480
           +TG I PIVKI+YNPSK+Y+ ++RRRTIEH S + ELRLL+CIHH D+TPSIIN+LE+SN
Sbjct: 421 VTGMISPIVKIIYNPSKKYKSTSRRRTIEHASPSGELRLLVCIHHHDNTPSIINLLEVSN 480

Query: 481 PTIKSPICFYLIHLLQLTGRASPLLIHHHHLRQRGSKH-YKLSDQIIKAFQIYQQFNYDK 540
           PT+KSPI FYL+HLLQLTGRASPL I H    Q GS H  +  + II AFQIYQ++NYDK
Sbjct: 481 PTLKSPIYFYLLHLLQLTGRASPLFIDH----QNGSHHDSQRFNPIINAFQIYQKYNYDK 540

Query: 541 VIMNAFTSVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESINPIRGVNKNILS 600
           V MNAFTS++PY +MHDDVCMLALEKRVAMVIVPFH+ R +NGI++S+N IRGVNKNILS
Sbjct: 541 VSMNAFTSIAPYVSMHDDVCMLALEKRVAMVIVPFHEWRAVNGIMDSMNQIRGVNKNILS 600

Query: 601 KAPCSVGILIDREMLPSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAEHPMVA 660
           KAPCSVGILIDR  L ST A +S+MNRVDLYKVGM+FVEG DDREALAYATRMAEHPMVA
Sbjct: 601 KAPCSVGILIDRVSLQST-AMMSMMNRVDLYKVGMLFVEGADDREALAYATRMAEHPMVA 660

Query: 661 LTVVRVIQPKRSSRHPADQDLDAEMINEFKLIMETSVLKHCTYEEEMASDCVGLINVIRT 720
           LTVVR++ P+  +   A+  LD+EM+NEFK IMETS ++HC YEEEM  DCVGLINVIR+
Sbjct: 661 LTVVRLMLPESKTERRAELILDSEMLNEFKRIMETSGIQHCVYEEEMVKDCVGLINVIRS 720

Query: 721 MEHDYDMILVGRRHDGDSALFVGLHEWNEFPELGYIGDMLASSDSTGAVAVLVVQQ 776
           ME +YD++LVGRRHDG+S LF+GL+EWNE+PELG IGDM AS+D +GAVA+LVVQQ
Sbjct: 721 MEQNYDLMLVGRRHDGESELFLGLNEWNEYPELGIIGDMFASTDFSGAVAMLVVQQ 771

BLAST of Bhi08G000351 vs. NCBI nr
Match: XP_022964746.1 (cation/H(+) antiporter 15-like [Cucurbita moschata])

HSP 1 Score: 1142.9 bits (2955), Expect = 0.0e+00
Identity = 579/775 (74.71%), Postives = 672/775 (86.71%), Query Frame = 0

Query: 1   MSTQAAHNGSWVCQPNRHYRSRGLFFGDSPFSFADSVLLAQLSLSSLLTSLLQCLLTPLG 60
           M+ +AAHNGSWVCQPN  YRSRG+FFGD+PFSF ++V LAQ+S+SSLLT LLQCLLTP+G
Sbjct: 1   MNIRAAHNGSWVCQPNERYRSRGIFFGDNPFSFGNTVFLAQISVSSLLTCLLQCLLTPIG 60

Query: 61  ESSFISQMLVGLALGPSFYGGDSPILEAIFPFKSFYVSETFAYFGCMVFMFLVGVKMDLS 120
           ESSF SQMLVGL LGPSF GG+SP+LE +FP+KSFYVSETFA+FGCM+FMFL+GVKMDL+
Sbjct: 61  ESSFFSQMLVGLVLGPSFNGGNSPLLEVVFPYKSFYVSETFAFFGCMIFMFLMGVKMDLT 120

Query: 121 LIAKSGKKALVIGVLAFVTPMVFNFLLTTYLKSNVEMDANLKNFLTTIGAFHASSSFHVI 180
           LI KSG KA+VIGVL F+ P++ N LL+ YLKS ++MD+NLK+ LT IGAF +SSSF+VI
Sbjct: 121 LITKSGTKAMVIGVLVFLFPLLINCLLSVYLKSTIDMDSNLKDSLTAIGAFQSSSSFYVI 180

Query: 181 ACLLSDLKLLNSDIGRLALSSSMISGTLSWIGLVASFTMRQTSMQQQDVLPWMALCVVCM 240
           AC+L DLKLLNS IGRLALSSSMISGTLSWI +V  FT+RQTSM+QQD LPWMA+CV CM
Sbjct: 181 ACVLEDLKLLNSHIGRLALSSSMISGTLSWISIVICFTLRQTSMEQQDALPWMAICVACM 240

Query: 241 IILVVYILRPIMIWIMEQTNLSGRPIKETYVLSLFLMLLFCALFSEFVGQHFILGPMILG 300
           IILV+YILRPIM WI+EQTN+SG PIKE+YV  +F+M+LFC+LFSEFVGQHF+LGPMILG
Sbjct: 241 IILVIYILRPIMFWIVEQTNISGGPIKESYVFLMFIMMLFCSLFSEFVGQHFLLGPMILG 300

Query: 301 LAVPDGPPLGSALVDKLDSFVSSIMLPCFFVISGARMNLSMFDMRSASIIHLLAFTAFMG 360
           LAVPDGPPLGSALVDKL+SFVSSIMLP +FVISGAR+NLS  DM+S  ++ LLAF + MG
Sbjct: 301 LAVPDGPPLGSALVDKLESFVSSIMLPFYFVISGARINLSKLDMKSIWVVQLLAFGSLMG 360

Query: 361 KLIGTMLPSLYCKMSLVDSLSLGLIMSAQGIADILGLQHGLLLYMIDQSSYSMTVVAMMV 420
           KLIG  LPSLYCKM LVDSL+LGLIMS QGI+DIL LQ GLLL MID +SYS+ V+AMMV
Sbjct: 361 KLIGATLPSLYCKMPLVDSLTLGLIMSTQGISDILFLQQGLLLQMIDLNSYSVMVLAMMV 420

Query: 421 MTGTICPIVKILYNPSKRYRCSARRRTIEHTSANAELRLLLCIHHQDDTPSIINMLELSN 480
           MTG   PIVK+LY PS +Y C+ RRRTIEHTS N ELR+LLCIHHQD+TPS+IN+LE+SN
Sbjct: 421 MTGATSPIVKMLYKPSNKYTCNRRRRTIEHTSPNGELRVLLCIHHQDNTPSVINLLEVSN 480

Query: 481 PTIKSPICFYLIHLLQLTGRASPLLIHHHHLRQRGSKHYKLSDQIIKAFQIYQQFNYDKV 540
           PTIKSPICFYLIHL+QLTGRASPLLIHHH  R R SK   LSDQII AFQ++QQ +YDKV
Sbjct: 481 PTIKSPICFYLIHLIQLTGRASPLLIHHHPNR-RSSKRCNLSDQIINAFQLFQQMSYDKV 540

Query: 541 IMNAFTSVSPYATMHDDVCMLALEKRVAMVIVPFHKRRTINGIVESINPIRGVNKNILSK 600
           IMNAFTSV+P+ATMHDDVCMLALEKRV+MVIVPFHKRRT+N    S+N I  VNKNILSK
Sbjct: 541 IMNAFTSVAPFATMHDDVCMLALEKRVSMVIVPFHKRRTMNAYEGSVNAISAVNKNILSK 600

Query: 601 APCSVGILIDREMLPSTAASVSLMNRVDLYKVGMIFVEGPDDREALAYATRMAEHPMVAL 660
           APCSVGILID  ++ +TA +V +MNR +LYKVG+IFV G DDREALAYATRM EHPMV L
Sbjct: 601 APCSVGILIDPMIMATTANTVPIMNRAELYKVGLIFVGGSDDREALAYATRMVEHPMVTL 660

Query: 661 TVVRVIQPKRSSRHPADQDLDAEMINEFKLIMETSVLKHCTYEEEMASDCVGLINVIRTM 720
           TVVR+I PK ++  PADQ+ D EM+NEFKLIM +S +KHC YEE  ASDCVGLI V+R M
Sbjct: 661 TVVRLIHPKGTTHQPADQEHDFEMLNEFKLIMSSSGIKHCFYEEVTASDCVGLIGVMRKM 720

Query: 721 EHDYDMILVGRRHDGDSALFVGLHEWNEFPELGYIGDMLASSDSTGAVAVLVVQQ 776
           EH+YD+ILVGRRHDGDSALFVGL+EW+E+PELG+IGDMLA+ DS     VLVVQQ
Sbjct: 721 EHNYDLILVGRRHDGDSALFVGLNEWHEYPELGFIGDMLATIDSPSDATVLVVQQ 774

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|Q9SIT5|CHX15_ARATH2.5e-13136.22Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1[more]
sp|Q9LMJ1|CHX14_ARATH6.2e-13034.01Cation/H(+) antiporter 14 OS=Arabidopsis thaliana OX=3702 GN=CHX14 PE=2 SV=1[more]
sp|O22920|CHX13_ARATH2.2e-11133.08Cation/H(+) symporter 13 OS=Arabidopsis thaliana OX=3702 GN=CHX13 PE=1 SV=1[more]
sp|Q9FFR9|CHX18_ARATH2.9e-10331.62Cation/H(+) antiporter 18 OS=Arabidopsis thaliana OX=3702 GN=CHX18 PE=2 SV=1[more]
sp|Q9FFB8|CHX3_ARATH6.1e-10133.86Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT2G13620.11.4e-13236.22cation/hydrogen exchanger 15[more]
AT1G06970.13.5e-13134.01cation/hydrogen exchanger 14[more]
AT2G30240.11.2e-11233.08Cation/hydrogen exchanger family protein[more]
AT5G41610.11.6e-10431.62cation/H+ exchanger 18[more]
AT5G22900.13.4e-10233.86cation/H+ exchanger 3[more]
Match NameE-valueIdentityDescription
tr|A0A0A0LT09|A0A0A0LT09_CUCSA0.0e+0088.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G025970 PE=4 SV=1[more]
tr|A0A1S3C4R5|A0A1S3C4R5_CUCME0.0e+0087.15cation/H(+) antiporter 15-like OS=Cucumis melo OX=3656 GN=LOC103497021 PE=4 SV=1[more]
tr|A0A2P6QKG9|A0A2P6QKG9_ROSCH4.1e-23554.40Putative cation/H+ exchanger OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr5g0071771... [more]
tr|A0A2N9EI55|A0A2N9EI55_FAGSY5.0e-23355.87Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS2212 PE=4 SV=1[more]
tr|A0A251NM28|A0A251NM28_PRUPE4.8e-22855.00Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G047700 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_004137561.10.0e+0088.00PREDICTED: cation/H(+) antiporter 15-like [Cucumis sativus] >KGN63917.1 hypothet... [more]
XP_008457299.20.0e+0087.15PREDICTED: cation/H(+) antiporter 15-like [Cucumis melo][more]
XP_022970379.10.0e+0075.35cation/H(+) antiporter 15-like [Cucurbita maxima][more]
XP_022949065.10.0e+0075.64cation/H(+) antiporter 15-like [Cucurbita moschata][more]
XP_022964746.10.0e+0074.71cation/H(+) antiporter 15-like [Cucurbita moschata][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0015299solute:proton antiporter activity
Vocabulary: Cellular Component
TermDefinition
GO:0016021integral component of membrane
Vocabulary: Biological Process
TermDefinition
GO:0006812cation transport
GO:0055085transmembrane transport
Vocabulary: INTERPRO
TermDefinition
IPR006153Cation/H_exchanger
IPR038770Na+/solute_symporter_sf
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902600 hydrogen ion transmembrane transport
biological_process GO:0006885 regulation of pH
biological_process GO:0006813 potassium ion transport
biological_process GO:0006814 sodium ion transport
biological_process GO:0030001 metal ion transport
biological_process GO:0015672 monovalent inorganic cation transport
biological_process GO:0055085 transmembrane transport
biological_process GO:0006812 cation transport
cellular_component GO:0012505 endomembrane system
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015299 solute:proton antiporter activity
molecular_function GO:0005451 monovalent cation:proton antiporter activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi08M000351Bhi08M000351mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038770Sodium/solute symporter superfamilyGENE3DG3DSA:1.20.1530.20coord: 35..442
e-value: 1.5E-59
score: 203.7
IPR006153Cation/H+ exchangerPFAMPF00999Na_H_Exchangercoord: 46..427
e-value: 4.0E-36
score: 124.5
NoneNo IPR availablePANTHERPTHR32468CATION/H + ANTIPORTERcoord: 20..780
NoneNo IPR availablePANTHERPTHR32468:SF33SUBFAMILY NOT NAMEDcoord: 20..780
NoneNo IPR availableCDDcd00293USP_Likecoord: 639..742
e-value: 2.99068E-4
score: 39.2754

The following gene(s) are paralogous to this gene:

None