Bhi07G000610 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi07G000610
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	3-oxo-5-alpha-steroid 4-dehydrogenase
Location	chr7 : 26115162 .. 26118038 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTCCATTCTACAAAAACCTCTGTTGTTGCTCTCTTTCGGTGCTTCAAAACGAGAGTCCCAGTTTTACAAATTTTCTAAAGAAACTTTATTCGGAGACTTCATCGATCCTGTCCACGGCGGCAATGGATATTTTATCGGCGTTTCTTTTCCCATCGCCGCCATCACTGTTCGTTAACGTGATGACAGTGGTGAGTTCGGCGGTGGTAGCGGTGATAGGGCTTTCGGAAGCGAGAGGAACGCATTTGCAGTATTCAAAGTTCTGGAATACGAATAATAGTGCTACGAAATCATCTAACTCAGAGCAACTCAGGATTTCTTCAAGATTGGGGATGCTCCTGCTCTATACACCGGCAGCTCTCGCCGGCGCTGCGTCGTTCTGGTTCTTTCCGGATGATGATTGGAGGATTGTTCTGCTTAAGTCCGCTCTCACTCTACATTTCTTTAAGCGGGACTTTGAGGTACTCTCTTCCTCTCTAATTTTGTTCAATCTAAACCAAGAAATAGATTTATCTTGTATAGTGAAATTTCGTGCTCTGTCTTTGTGATTAATACTTAACGATTTGCTTTCTTGTGACGAATTAGTTGTTCAATCTTGTTTGGTTCGTTTTTTTGTTCTTGATTGAACGAGAATCGGGGATTTGTTGAGAACGTGTCGATCTTGCTCTGTTTATTCGATTCGATTTGAATTTATTTGATTCATATTATTGTGAGTCATTATTTATTCAAAATGTTAAACTAGTTTTCAGATCTAGTTTTTGAGATGTTAGTTTTCAAATCTCGAGAGTAAAAAGAATTTTTAATTTTTTTCAAATATTAGATAATCTAACCAAATTATATACATTTTCAAATTTTTATTTATCTTTATATTCTGATGTAATTTATGAGCACTTTTTGTTTACATAATTATATAAATTTCTAAATTTCTAAATGAATTTACAAATTAGATTTTTTCAAATGAATGATTGAAAAAGGTAAGATATGAAAAATATATAATATCTCGATCGAGAATTCACCAATCGATATTAAAAAAATATGAAAAGATTAGACTTTTGTAATCTTTGCGAGATATATTGAGAAAGACCCAAACCATCTATAAGTAAGAAAAAAATTAATTTTTTTAATTAAAATCTCGATGAATTATTTGAATAGTTTTAAAAAAGATGCCAATTTTATACAATGAAAACTTGTTTATGACAATTTCCCTCTTGTTTAAGACATTTCTATTTCTTTTTGTAGTAAATTTTTAGTCTAAGAATAATACTTTTTTCCTTACTAGTGCAGGGATCTCCTTCTCCCTTACGGTTTAGCTGAATTTAAAATAATAAAAATAATAATTAATTGAAAAAAAATCGAACTTCACAGTTCGTCTAAAAAATACTCCTAAAACATCCTACGATAATTAAAAAAAAAAAAAAAAAATTTTGTAAATTTATTGTTGGCCCTATATTTATATCCACTTCAAAATTGTTTCTATGTGTAATTTAAATCCAAATAATATGGATTAGGTAAGTTGTTTGAAATCTTAGGGAGTGTTGTTTGACGAATGTAAAAACTTCAATAGTGTTTATTATTATAATTGAAGTTGAGTTTTTTATATTTATAAAGTGGGCCAAATCTCTTCAAAGTAGTTTTTTTTTTTTTTTTAAAAAAAGTGCTTGAGTTGGGTTCATAACAACTTGTCTAGAGTTTTTTTAGTGTATAAGATATCACGATATCACAAAACTATACCCAACTCAAGCAAATCCATAAACAAGATGTTTTTATGTGTTCTTCACATTCTCTATGGAAAAAATCCTACAGGTATTGTTTGTTCACAAGTACAGTGGCTACATGATACTTGATTCAGCAGTTATAATCTCCCTCAGCTACTTCTCTTCTACAGCACTCATGATATACACTCAACAGCTTTCAAAAGCACTTGTAGAGCCATCCATTGATCTGAAAAACATTGGAATTGCTGTGTTTGTGATAGGCATTATTGGGAACATGTACCACCATTATATTTTGTCTCAAACTAGAAAAAAGGGAGAAACAGGCTACAAAATTCCCAAAGGAGGCCTCTTTAGCATTGTCATTTGCCCTCACTATTTGTTTGAAATAACTGTATATTTCGGGTTCGCTCTCATTTCTCAGACACTCTACTCATTGTGCTTTGCCATTGCCACTGCTATCTATTTGGGTGGAAGAAGCTACGCCACTAGGAAATGGTATGTCTCTAAATTTGAGGACTTCCCTCAGCATATCAAAGCCCTTATTCCTTTCGTATTCTAGTCTTTGGTTAAATATTCAACTTTGTTCTCATTTGAAAAATACCCCCTTATACTTTTTAAAAGTTATGCAATATTATTCTTGACATTTCATAGAACGGAAAATTGGATCAATACACTTTTTAAAGTCTCAAGGTAATATTGCAACTTTGGAAAGCGTACATGGTACTTTCAAAATTAAACAAAGAACAAAGTTGAAGGTATTTTCTATAATTTATTTTGTTATTGTTCTTCATGTTGTATCATTTGTATTCGTCCAACGTCTCTGTATCATTTATATTCGTCCAATATCTCTTTGTAGCATCATGTATAATGTTAAAATTTGTGTTTTGTGGTCGGCTTTATGAGAAAGATAAACCATGGAAGTTGTTGCATTGTAGCGTCTACGTTGAATAATTTTCCATACATTTGGAATCCTCCTATTATTTTAGGAGGAGCTTTGGGTCATTTCACTTTATGTAATCACTGTACTGTATCAAATTGTGTCTTTGGCCATAATTCAATAATTTTCTTTTTTCTATTTGAAGAAAATACCATTGACTTGTATGGGATTGCTGTTATAAAATTTATAATCACATGTCACAGTATGGGGCCATTTTTAATTGTTCA

mRNA sequence

GTTCCATTCTACAAAAACCTCTGTTGTTGCTCTCTTTCGGTGCTTCAAAACGAGAGTCCCAGTTTTACAAATTTTCTAAAGAAACTTTATTCGGAGACTTCATCGATCCTGTCCACGGCGGCAATGGATATTTTATCGGCGTTTCTTTTCCCATCGCCGCCATCACTGTTCGTTAACGTGATGACAGTGGTGAGTTCGGCGGTGGTAGCGGTGATAGGGCTTTCGGAAGCGAGAGGAACGCATTTGCAGTATTCAAAGTTCTGGAATACGAATAATAGTGCTACGAAATCATCTAACTCAGAGCAACTCAGGATTTCTTCAAGATTGGGGATGCTCCTGCTCTATACACCGGCAGCTCTCGCCGGCGCTGCGTCGTTCTGGTTCTTTCCGGATGATGATTGGAGGATTGTTCTGCTTAAGTCCGCTCTCACTCTACATTTCTTTAAGCGGGACTTTGAGGTATTGTTTGTTCACAAGTACAGTGGCTACATGATACTTGATTCAGCAGTTATAATCTCCCTCAGCTACTTCTCTTCTACAGCACTCATGATATACACTCAACAGCTTTCAAAAGCACTTGTAGAGCCATCCATTGATCTGAAAAACATTGGAATTGCTGTGTTTGTGATAGGCATTATTGGGAACATGTACCACCATTATATTTTGTCTCAAACTAGAAAAAAGGGAGAAACAGGCTACAAAATTCCCAAAGGAGGCCTCTTTAGCATTGTCATTTGCCCTCACTATTTGTTTGAAATAACTGTATATTTCGGGTTCGCTCTCATTTCTCAGACACTCTACTCATTGTGCTTTGCCATTGCCACTGCTATCTATTTGGGTGGAAGAAGCTACGCCACTAGGAAATGGTATGTCTCTAAATTTGAGGACTTCCCTCAGCATATCAAAGCCCTTATTCCTTTCGTATTCTAGTCTTTGGTTAAATATTCAACTTTGTTCTCATTTGAAAAATACCCCCTTATACTTTTTAAAAGTTATGCAATATTATTCTTGACATTTCATAGAACGGAAAATTGGATCAATACACTTTTTAAAGTCTCAAGGTAATATTGCAACTTTGGAAAGCGTACATGGTACTTTCAAAATTAAACAAAGAACAAAGTTGAAGGTATTTTCTATAATTTATTTTGTTATTGTTCTTCATGTTGTATCATTTGTATTCGTCCAACGTCTCTGTATCATTTATATTCGTCCAATATCTCTTTGTAGCATCATGTATAATGTTAAAATTTGTGTTTTGTGGTCGGCTTTATGAGAAAGATAAACCATGGAAGTTGTTGCATTGTAGCGTCTACGTTGAATAATTTTCCATACATTTGGAATCCTCCTATTATTTTAGGAGGAGCTTTGGGTCATTTCACTTTATGTAATCACTGTACTGTATCAAATTGTGTCTTTGGCCATAATTCAATAATTTTCTTTTTTCTATTTGAAGAAAATACCATTGACTTGTATGGGATTGCTGTTATAAAATTTATAATCACATGTCACAGTATGGGGCCATTTTTAATTGTTCA

Coding sequence (CDS)

ATGGATATTTTATCGGCGTTTCTTTTCCCATCGCCGCCATCACTGTTCGTTAACGTGATGACAGTGGTGAGTTCGGCGGTGGTAGCGGTGATAGGGCTTTCGGAAGCGAGAGGAACGCATTTGCAGTATTCAAAGTTCTGGAATACGAATAATAGTGCTACGAAATCATCTAACTCAGAGCAACTCAGGATTTCTTCAAGATTGGGGATGCTCCTGCTCTATACACCGGCAGCTCTCGCCGGCGCTGCGTCGTTCTGGTTCTTTCCGGATGATGATTGGAGGATTGTTCTGCTTAAGTCCGCTCTCACTCTACATTTCTTTAAGCGGGACTTTGAGGTATTGTTTGTTCACAAGTACAGTGGCTACATGATACTTGATTCAGCAGTTATAATCTCCCTCAGCTACTTCTCTTCTACAGCACTCATGATATACACTCAACAGCTTTCAAAAGCACTTGTAGAGCCATCCATTGATCTGAAAAACATTGGAATTGCTGTGTTTGTGATAGGCATTATTGGGAACATGTACCACCATTATATTTTGTCTCAAACTAGAAAAAAGGGAGAAACAGGCTACAAAATTCCCAAAGGAGGCCTCTTTAGCATTGTCATTTGCCCTCACTATTTGTTTGAAATAACTGTATATTTCGGGTTCGCTCTCATTTCTCAGACACTCTACTCATTGTGCTTTGCCATTGCCACTGCTATCTATTTGGGTGGAAGAAGCTACGCCACTAGGAAATGGTATGTCTCTAAATTTGAGGACTTCCCTCAGCATATCAAAGCCCTTATTCCTTTCGTATTCTAG

Protein sequence

MDILSAFLFPSPPSLFVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTNNSATKSSNSEQLRISSRLGMLLLYTPAALAGAASFWFFPDDDWRIVLLKSALTLHFFKRDFEVLFVHKYSGYMILDSAVIISLSYFSSTALMIYTQQLSKALVEPSIDLKNIGIAVFVIGIIGNMYHHYILSQTRKKGETGYKIPKGGLFSIVICPHYLFEITVYFGFALISQTLYSLCFAIATAIYLGGRSYATRKWYVSKFEDFPQHIKALIPFVF

Homology

[Close]

BLAST of Bhi07G000610 vs. Swiss-Prot
Match: sp|Q28891|S5A1_MACFA (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.2e-18
Identity = 45/107 (42.06%), Postives = 68/107 (63.55%), Query Frame = 0


Query: 162 IGIAVFVIGIIGNMYHHYILSQTRKKGETGYKIPKGGLFSIVICPHYLFEITVYFGFALI 221
           IG  +++ G++ N++  +IL   RK G+TGYKIP+GGLF  V   +Y  EI  + G+AL 
Sbjct: 157 IGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 216

Query: 222 SQTLYSLCFAIATAIYLGGRSYATRKWYVSKFEDFPQHIKALIPFVF 269
           S ++    FA  T  +L GR+    +WY+ KFE++P+  K LIPF+F
Sbjct: 217 SWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKFEEYPKFRKILIPFLF 263

[Close]

BLAST of Bhi07G000610 vs. Swiss-Prot
Match: sp|O18765|S5A2_PIG (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Sus scrofa OX=9823 GN=SRD5A2 PE=2 SV=2)

HSP 1 Score: 95.1 bits (235), Expect = 1.2e-18
Identity = 47/108 (43.52%), Postives = 74/108 (68.52%), Query Frame = 0


Query: 161 NIGIAVFVIGIIGNMYHHYILSQTRKKGETGYKIPKGGLFSIVICPHYLFEITVYFGFAL 220
           ++G+ +F++G+  N++  YIL Q RK GE  YKIP+GGLF+ V   ++L EI  + G+AL
Sbjct: 147 SLGVFLFILGMGINIHSDYILRQLRKPGEVIYKIPQGGLFTYVSGANFLGEIIEWIGYAL 206

Query: 221 ISQTLYSLCFAIATAIYLGGRSYATRKWYVSKFEDFPQHIKALIPFVF 269
            + +L +L FA  +  +LG R++   ++YV  FED+P+  KALIPF+F
Sbjct: 207 ATWSLPALAFAFFSLCFLGLRAFHHHRFYVKMFEDYPKSRKALIPFIF 254

[Close]

BLAST of Bhi07G000610 vs. Swiss-Prot
Match: sp|Q68FF9|S5A1_MOUSE (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 SV=2)

HSP 1 Score: 94.0 bits (232), Expect = 2.8e-18
Identity = 46/105 (43.81%), Postives = 68/105 (64.76%), Query Frame = 0


Query: 163 GIAVFVIGIIGNMYHHYILSQTRKKGETGYKIPKGGLFSIVICPHYLFEITVYFGFALIS 222
           G A++++G++ N++  +IL   RK GETGYKIP+GGLF  V   +Y  E+  + GFAL S
Sbjct: 150 GFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSSANYFGELVEWCGFALAS 209

Query: 223 QTLYSLCFAIATAIYLGGRSYATRKWYVSKFEDFPQHIKALIPFV 268
            +L  + FA+ T   L  R+    +WY+ KFED+P+  K LIPF+
Sbjct: 210 WSLQGVVFALFTLCALFTRARQHHQWYLEKFEDYPKTRKILIPFL 254

[Close]

BLAST of Bhi07G000610 vs. Swiss-Prot
Match: sp|P18405|S5A1_HUMAN (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 3.6e-18
Identity = 44/107 (41.12%), Postives = 68/107 (63.55%), Query Frame = 0


Query: 162 IGIAVFVIGIIGNMYHHYILSQTRKKGETGYKIPKGGLFSIVICPHYLFEITVYFGFALI 221
           IG  +++ G++ N++  +IL   RK G+TGYKIP+GGLF  V   +Y  EI  + G+AL 
Sbjct: 153 IGFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 212

Query: 222 SQTLYSLCFAIATAIYLGGRSYATRKWYVSKFEDFPQHIKALIPFVF 269
           S ++    FA  T  +L GR+    +WY+ KFE++P+  K +IPF+F
Sbjct: 213 SWSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPFLF 259

[Close]

BLAST of Bhi07G000610 vs. Swiss-Prot
Match: sp|P24008|S5A1_RAT (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 PE=1 SV=2)

HSP 1 Score: 93.6 bits (231), Expect = 3.6e-18
Identity = 47/105 (44.76%), Postives = 67/105 (63.81%), Query Frame = 0


Query: 163 GIAVFVIGIIGNMYHHYILSQTRKKGETGYKIPKGGLFSIVICPHYLFEITVYFGFALIS 222
           G A++++G++ N++  +IL   RK GETGYKIP+GGLF  V   +Y  E+  + GFAL S
Sbjct: 154 GFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSAANYFGELVEWCGFALAS 213

Query: 223 QTLYSLCFAIATAIYLGGRSYATRKWYVSKFEDFPQHIKALIPFV 268
            +L  + FA+ T   L  R+    +WY  KFED+P+  K LIPFV
Sbjct: 214 WSLQGVVFALFTLSTLLTRAKQHHQWYHEKFEDYPKSRKILIPFV 258

[Close]

BLAST of Bhi07G000610 vs. TAIR10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 323.2 bits (827), Expect = 1.6e-88
Identity = 165/269 (61.34%), Postives = 210/269 (78.07%), Query Frame = 0


Query: 1   MDILSAFLFPSPPSLFVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTNNSATKSSNSE 60
           M+++++F++P PPS+ +N M+VV  A +A IG SE RG HL+YSKF   ++S+ +     
Sbjct: 1   MEMVTSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGNHLKYSKF-GVSSSSPQPQKER 60

Query: 61  QLRISSRLGMLLLYTPAALAGAASFWFFPDDDWRIVLLKSALTLHFFKRDFEVLFVHKYS 120
              ISSR GMLLLYTPA LA A+SF+  P DD R +LLKSAL LHFFKR FEVLF+HKYS
Sbjct: 61  FGSISSRNGMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFEVLFIHKYS 120

Query: 121 GYMILDSAVIISLSYFSSTALMIYTQQLSKALVEPSIDLKNIGIAVFVIGIIGNMYHHYI 180
           G M +DSA+ IS SYFSSTALM+Y+Q L+  L EPS D+K  G+ +FV+GI+GN+YHH +
Sbjct: 121 GGMAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVL 180

Query: 181 LSQTRKK-GETGYKIPKGGLFSIVICPHYLFEITVYFGFALISQTLYSLCFAIATAIYLG 240
           L++ RK+ G+  YKIPKGGLF I+ICPHYLFEI V++ F LISQT+YS  FA+ T +YL 
Sbjct: 181 LAKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLI 240

Query: 241 GRSYATRKWYVSKFEDFPQHIKALIPFVF 269
           GRSYATR WY+SKF+DFP+HIKALIPFVF
Sbjct: 241 GRSYATRTWYLSKFDDFPKHIKALIPFVF 268

[Close]

BLAST of Bhi07G000610 vs. TAIR10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 78.2 bits (191), Expect = 8.7e-15
Identity = 40/108 (37.04%), Postives = 67/108 (62.04%), Query Frame = 0


Query: 162 IGIAVFVIGIIGNMYHHYILSQTRKKGETGYKIPKGGLFSIVICPHYLFEITVYFGFALI 221
           IG+ VF+ G+  N+     L + +K+   GY IP+GG F +V CP+Y  E   + G+A++
Sbjct: 155 IGMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVM 214

Query: 222 SQTLYSLCFAIATAIYLGGRSYATRKWYVSKF-EDFPQHIKALIPFVF 269
           + +   + F + T   L  R+ A+ KWY++KF E++P+  KA+IPFV+
Sbjct: 215 TWSWAGIGFFLYTCSNLFPRARASHKWYIAKFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of Bhi07G000610 vs. TAIR10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 62.8 bits (151), Expect = 3.8e-10
Identity = 44/136 (32.35%), Postives = 67/136 (49.26%), Query Frame = 0


Query: 105 HFFKRDFEVLFVHKYSGYMILDSAVIISLSYFSSTALMIYTQQLSKALVEPSIDLK-NIG 164
           H+FKR  E  FVH++S        V  + +Y+ S    I    ++  L  P  DL+  IG
Sbjct: 141 HYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYI-AYYVNHPLYTPVSDLQMKIG 200

Query: 165 IAVFVIGIIGNMYHHYILSQTR-KKGETGYKIPKGGLFSIVICPHYLFEITVYFGFALIS 224
               ++  + N Y H +L   R   G  GY+IP+G LF+IV C +Y  EI  + GF + +
Sbjct: 201 FGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNIAT 260

Query: 225 QTLYSLCFAIATAIYL 239
           QT+    F    A+ +
Sbjct: 261 QTIAGYVFLAVAALIM 275

[Close]

BLAST of Bhi07G000610 vs. TAIR10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 59.3 bits (142), Expect = 4.2e-09
Identity = 41/117 (35.04%), Postives = 58/117 (49.57%), Query Frame = 0


Query: 159 LKNIGIAVFVIGIIGNMYHHYILSQTRKKGETG--YKIPKGGLFSIVICPHYLFEITVYF 218
           L+ IG A+F+ G I     H IL   R+       Y IP G  F +V CPH+L EI +Y 
Sbjct: 207 LQWIGGAIFLWGWIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYL 266

Query: 219 GFALISQ----TLYSLC-FAIATAIYLGGRSYATRKWYVSKFEDFPQHIKALIPFVF 269
           G  + S     +++ L  F  A   Y  G    T +WY+ KFE++P    A+ P V+
Sbjct: 267 GLLISSGGTDISIWLLFGFVAANLTYAAGE---THRWYLQKFENYPASRHAIFPHVY 320

[Close]

BLAST of Bhi07G000610 vs. TAIR10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 58.9 bits (141), Expect = 5.5e-09
Identity = 58/220 (26.36%), Postives = 86/220 (39.09%), Query Frame = 0


Query: 93  WRIVLLKSALTLHFFKRDFEVLFVHKYSGYMILDSAVIISLSYFSSTALMIYTQQLSKAL 152
           WR V L   + +H  +R  E  +V KYS      SA +  L YF+     +       + 
Sbjct: 132 WRAVFLLLLMEIHVLRRLIESFYVFKYS-----PSARMHILGYFAGLFFYVTAPLSLCSN 191

Query: 153 VEPSI-------------------------------------DLKNIGIAVFVIGIIGNM 212
           + P +                                      L+ IG A+F+ G I   
Sbjct: 192 IAPEVAGFVGNQVAEFIANGKSHTSAPEFNLLSSISPLMKLGSLQWIGGAIFLWGWIHQR 251

Query: 213 YHHYILSQTRKKGETG--YKIPKGGLFSIVICPHYLFEITVYFGFALISQ----TLYSLC 269
             H IL   R+       Y IP G  F +V  PH+L EI +Y G  + S     T++ L 
Sbjct: 252 RCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDITIWLLF 311

[Close]

BLAST of Bhi07G000610 vs. TrEMBL
Match: tr|A0A1S3BGE8|A0A1S3BGE8_CUCME (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo OX=3656 GN=LOC103489361 PE=4 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 3.1e-131
Identity = 243/268 (90.67%), Postives = 253/268 (94.40%), Query Frame = 0


Query: 1   MDILSAFLFPSPPSLFVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTNNSATKSSNSE 60
           M+ LS F FP PPSL VNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNT+NSATKSSNSE
Sbjct: 1   MNALSVFFFPMPPSLLVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTSNSATKSSNSE 60

Query: 61  QLRISSRLGMLLLYTPAALAGAASFWFFPDDDWRIVLLKSALTLHFFKRDFEVLFVHKYS 120
           QLRISSRLGMLLLYTPAALAGAASFWFFP+DDWR++LLKSALTLHFFKRDFEVLFVHKYS
Sbjct: 61  QLRISSRLGMLLLYTPAALAGAASFWFFPNDDWRVLLLKSALTLHFFKRDFEVLFVHKYS 120

Query: 121 GYMILDSAVIISLSYFSSTALMIYTQQLSKALVEPSIDLKNIGIAVFVIGIIGNMYHHYI 180
           G M+LDSA IISLSYFSSTALMIY QQLSKAL EP+IDLKNIGIA+F+IGIIGNMYHH +
Sbjct: 121 GKMVLDSASIISLSYFSSTALMIYAQQLSKALPEPTIDLKNIGIALFLIGIIGNMYHHIL 180

Query: 181 LSQTRKKGETGYKIPKGGLFSIVICPHYLFEITVYFGFALISQTLYSLCFAIATAIYLGG 240
           LSQTRKKGETGYKIPKGGLF IVICPHYLFEITVYFGFALISQT YS  FAIATAIYLGG
Sbjct: 181 LSQTRKKGETGYKIPKGGLFGIVICPHYLFEITVYFGFALISQTFYSFFFAIATAIYLGG 240

Query: 241 RSYATRKWYVSKFEDFPQHIKALIPFVF 269
           RSYAT KWYVSKFEDFPQHIKALIPFVF
Sbjct: 241 RSYATWKWYVSKFEDFPQHIKALIPFVF 268

[Close]

BLAST of Bhi07G000610 vs. TrEMBL
Match: tr|A0A0A0KRA7|A0A0A0KRA7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G612890 PE=4 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 7.1e-128
Identity = 236/268 (88.06%), Postives = 249/268 (92.91%), Query Frame = 0


Query: 1   MDILSAFLFPSPPSLFVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTNNSATKSSNSE 60
           M+ LSAF FP PPSL VNVMTVVSS VVAVIG SEARGTHLQYSKFWNT+NSATKSSNSE
Sbjct: 7   MNTLSAFFFPLPPSLLVNVMTVVSSVVVAVIGFSEARGTHLQYSKFWNTSNSATKSSNSE 66

Query: 61  QLRISSRLGMLLLYTPAALAGAASFWFFPDDDWRIVLLKSALTLHFFKRDFEVLFVHKYS 120
           QLRISSRLGMLLLYTPA LAGAASFWF P+DD R++LLKSALT HFFKRDFEVLFVHKYS
Sbjct: 67  QLRISSRLGMLLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVLFVHKYS 126

Query: 121 GYMILDSAVIISLSYFSSTALMIYTQQLSKALVEPSIDLKNIGIAVFVIGIIGNMYHHYI 180
           G M++DSA+IISLSYFSSTALMIY QQ SKAL EP+IDLKNIGIAVFVIGIIGNMYHH +
Sbjct: 127 GKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGNMYHHIL 186

Query: 181 LSQTRKKGETGYKIPKGGLFSIVICPHYLFEITVYFGFALISQTLYSLCFAIATAIYLGG 240
           LSQTRKKGETGYKIPKGGLF I+ICPHY+FEITVYFGFALISQT Y+  FAIATAIYLGG
Sbjct: 187 LSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIATAIYLGG 246

Query: 241 RSYATRKWYVSKFEDFPQHIKALIPFVF 269
           RSYATRKWYVSKFEDFPQHIKALIPFVF
Sbjct: 247 RSYATRKWYVSKFEDFPQHIKALIPFVF 274

[Close]

BLAST of Bhi07G000610 vs. TrEMBL
Match: tr|A0A1S3BFS0|A0A1S3BFS0_CUCME (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucumis melo OX=3656 GN=LOC103489360 PE=4 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 7.1e-104
Identity = 193/268 (72.01%), Postives = 221/268 (82.46%), Query Frame = 0


Query: 1   MDILSAFLFPSPPSLFVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTNNSATKSSNSE 60
           MDILSAFLFP PPS FVNVMTVVSS  +A+ GLSE +G HLQYSKFWN N+ +  S  ++
Sbjct: 11  MDILSAFLFPPPPSTFVNVMTVVSSTSLAMAGLSEIKGKHLQYSKFWNANSGSKLS--AQ 70

Query: 61  QLRISSRLGMLLLYTPAALAGAASFWFFPDDDWRIVLLKSALTLHFFKRDFEVLFVHKYS 120
           + ++SS+ GMLL+YTPA LAGAASFW FP  D R  LLKSALTLHFFKR FEVLF+HKYS
Sbjct: 71  ESKLSSKYGMLLVYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVLFIHKYS 130

Query: 121 GYMILDSAVIISLSYFSSTALMIYTQQLSKALVEPSIDLKNIGIAVFVIGIIGNMYHHYI 180
             M+L +A+ IS SYF STA+MIYTQ LS+ L EP IDLKNIG+ +F+ GIIGN YHHY+
Sbjct: 131 SKMVLKTAIQISFSYFLSTAIMIYTQHLSQGLPEPPIDLKNIGVGLFLTGIIGNFYHHYL 190

Query: 181 LSQTRKKGETGYKIPKGGLFSIVICPHYLFEITVYFGFALISQTLYSLCFAIATAIYLGG 240
           LSQTRK+ ET YKIPKGGLFS VICPHYLFEI  YFGFA ISQ LY LCF+I TAIYL G
Sbjct: 191 LSQTRKQEETAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGTAIYLAG 250

Query: 241 RSYATRKWYVSKFEDFPQHIKALIPFVF 269
           RSYATRKWY+SKFE+FP H+KAL+PFVF
Sbjct: 251 RSYATRKWYISKFENFPNHVKALLPFVF 276

[Close]

BLAST of Bhi07G000610 vs. TrEMBL
Match: tr|A0A0A0KWK8|A0A0A0KWK8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G612900 PE=4 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 6.6e-102
Identity = 189/269 (70.26%), Postives = 224/269 (83.27%), Query Frame = 0


Query: 1   MDILSAFLFPSPPSLFVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTNNSATKSSNSE 60
           MD LS F+FP P S FVNVMTVVSSA+VAV+GLSEARG HL+YSKFW++NN+ T   + +
Sbjct: 1   MDTLSEFVFPPPASAFVNVMTVVSSAIVAVVGLSEARGKHLKYSKFWSSNNANT---DRK 60

Query: 61  QLRISSRLGMLLLYTPAALAGAASFWFFPDDDWRIVLLKSALTLHFFKRDFEVLFVHKYS 120
              +S++LGML+ Y PA LAG  S W FP +D RI+LLKSAL LHFFKR+ EVLF+HKYS
Sbjct: 61  SFNLSAKLGMLVAYIPAFLAGVVSLWLFPSEDRRILLLKSALVLHFFKRNLEVLFLHKYS 120

Query: 121 GYMILDSAVIISLSYFSSTALMIYTQQLSKALV-EPSIDLKNIGIAVFVIGIIGNMYHHY 180
             M +DS + IS SYFSSTA+MIYTQ LS+ L+ EP IDLK+IG+ +F+IGIIGN YHHY
Sbjct: 121 SKMAVDSVITISFSYFSSTAIMIYTQHLSQGLLREPPIDLKSIGVGLFLIGIIGNFYHHY 180

Query: 181 ILSQTRKKGETGYKIPKGGLFSIVICPHYLFEITVYFGFALISQTLYSLCFAIATAIYLG 240
           +LSQTRK+GET YKIPKGGLFS++ICPHYLFEI  +FGFA ISQT+YSL FA ATA+YL 
Sbjct: 181 LLSQTRKQGETSYKIPKGGLFSLIICPHYLFEIIEFFGFAFISQTIYSLFFAFATALYLS 240

Query: 241 GRSYATRKWYVSKFEDFPQHIKALIPFVF 269
           GRSYATRKWYVSKFEDFP H+KAL+PFVF
Sbjct: 241 GRSYATRKWYVSKFEDFPIHVKALLPFVF 266

[Close]

BLAST of Bhi07G000610 vs. TrEMBL
Match: tr|A0A1S3BGK3|A0A1S3BGK3_CUCME (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo OX=3656 GN=LOC103489362 PE=4 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 2.1e-100
Identity = 188/269 (69.89%), Postives = 221/269 (82.16%), Query Frame = 0


Query: 1   MDILSAFLFPSPPSLFVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTNNSATKSSNSE 60
           MD LS F+FP P S FVNVMTVVSSA+VAV+GLSEARG HLQYSKFWN NN+A  +++ +
Sbjct: 1   MDALSEFVFPPPSSAFVNVMTVVSSAIVAVVGLSEARGKHLQYSKFWNNNNNA--NTDRK 60

Query: 61  QLRISSRLGMLLLYTPAALAGAASFWFFPDDDWRIVLLKSALTLHFFKRDFEVLFVHKYS 120
              +S++LGMLL Y PA L G  SFW FP++D RIV+LKSAL LHFFKR+ EVLF+HKYS
Sbjct: 61  SFNLSAKLGMLLAYMPAFLVGVVSFWLFPNEDRRIVVLKSALVLHFFKRNLEVLFLHKYS 120

Query: 121 GYMILDSAVIISLSYFSSTALMIYTQQLSKALV-EPSIDLKNIGIAVFVIGIIGNMYHHY 180
             M + S + IS SYFSSTA+MIYTQ LS+ L+ EP IDLK IG+ +F+IGI+GN YHHY
Sbjct: 121 SKMAIGSVITISFSYFSSTAIMIYTQHLSQGLLPEPPIDLKIIGVGLFLIGIVGNFYHHY 180

Query: 181 ILSQTRKKGETGYKIPKGGLFSIVICPHYLFEITVYFGFALISQTLYSLCFAIATAIYLG 240
           +LS TRK GET YKIP+GGLF+ VICPHYLFEI  +FGFA ISQT+YSL FA ATA+YL 
Sbjct: 181 LLSGTRKGGETSYKIPEGGLFNFVICPHYLFEIIEFFGFAFISQTIYSLFFAFATALYLT 240

Query: 241 GRSYATRKWYVSKFEDFPQHIKALIPFVF 269
           GRSYATRKWYVSKFEDFP H+KAL+PFVF
Sbjct: 241 GRSYATRKWYVSKFEDFPIHVKALLPFVF 267

[Close]

BLAST of Bhi07G000610 vs. NCBI nr
Match: XP_008446723.1 (PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo])

HSP 1 Score: 476.9 bits (1226), Expect = 4.6e-131
Identity = 243/268 (90.67%), Postives = 253/268 (94.40%), Query Frame = 0


Query: 1   MDILSAFLFPSPPSLFVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTNNSATKSSNSE 60
           M+ LS F FP PPSL VNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNT+NSATKSSNSE
Sbjct: 1   MNALSVFFFPMPPSLLVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTSNSATKSSNSE 60

Query: 61  QLRISSRLGMLLLYTPAALAGAASFWFFPDDDWRIVLLKSALTLHFFKRDFEVLFVHKYS 120
           QLRISSRLGMLLLYTPAALAGAASFWFFP+DDWR++LLKSALTLHFFKRDFEVLFVHKYS
Sbjct: 61  QLRISSRLGMLLLYTPAALAGAASFWFFPNDDWRVLLLKSALTLHFFKRDFEVLFVHKYS 120

Query: 121 GYMILDSAVIISLSYFSSTALMIYTQQLSKALVEPSIDLKNIGIAVFVIGIIGNMYHHYI 180
           G M+LDSA IISLSYFSSTALMIY QQLSKAL EP+IDLKNIGIA+F+IGIIGNMYHH +
Sbjct: 121 GKMVLDSASIISLSYFSSTALMIYAQQLSKALPEPTIDLKNIGIALFLIGIIGNMYHHIL 180

Query: 181 LSQTRKKGETGYKIPKGGLFSIVICPHYLFEITVYFGFALISQTLYSLCFAIATAIYLGG 240
           LSQTRKKGETGYKIPKGGLF IVICPHYLFEITVYFGFALISQT YS  FAIATAIYLGG
Sbjct: 181 LSQTRKKGETGYKIPKGGLFGIVICPHYLFEITVYFGFALISQTFYSFFFAIATAIYLGG 240

Query: 241 RSYATRKWYVSKFEDFPQHIKALIPFVF 269
           RSYAT KWYVSKFEDFPQHIKALIPFVF
Sbjct: 241 RSYATWKWYVSKFEDFPQHIKALIPFVF 268

[Close]

BLAST of Bhi07G000610 vs. NCBI nr
Match: XP_011655823.1 (PREDICTED: steroid 5-alpha-reductase DET2 [Cucumis sativus] >KGN52160.1 hypothetical protein Csa_5G612890 [Cucumis sativus])

HSP 1 Score: 465.7 bits (1197), Expect = 1.1e-127
Identity = 236/268 (88.06%), Postives = 249/268 (92.91%), Query Frame = 0


Query: 1   MDILSAFLFPSPPSLFVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTNNSATKSSNSE 60
           M+ LSAF FP PPSL VNVMTVVSS VVAVIG SEARGTHLQYSKFWNT+NSATKSSNSE
Sbjct: 7   MNTLSAFFFPLPPSLLVNVMTVVSSVVVAVIGFSEARGTHLQYSKFWNTSNSATKSSNSE 66

Query: 61  QLRISSRLGMLLLYTPAALAGAASFWFFPDDDWRIVLLKSALTLHFFKRDFEVLFVHKYS 120
           QLRISSRLGMLLLYTPA LAGAASFWF P+DD R++LLKSALT HFFKRDFEVLFVHKYS
Sbjct: 67  QLRISSRLGMLLLYTPAVLAGAASFWFLPNDDLRVLLLKSALTFHFFKRDFEVLFVHKYS 126

Query: 121 GYMILDSAVIISLSYFSSTALMIYTQQLSKALVEPSIDLKNIGIAVFVIGIIGNMYHHYI 180
           G M++DSA+IISLSYFSSTALMIY QQ SKAL EP+IDLKNIGIAVFVIGIIGNMYHH +
Sbjct: 127 GKMVIDSALIISLSYFSSTALMIYAQQQSKALPEPTIDLKNIGIAVFVIGIIGNMYHHIL 186

Query: 181 LSQTRKKGETGYKIPKGGLFSIVICPHYLFEITVYFGFALISQTLYSLCFAIATAIYLGG 240
           LSQTRKKGETGYKIPKGGLF I+ICPHY+FEITVYFGFALISQT Y+  FAIATAIYLGG
Sbjct: 187 LSQTRKKGETGYKIPKGGLFGIIICPHYMFEITVYFGFALISQTFYAFFFAIATAIYLGG 246

Query: 241 RSYATRKWYVSKFEDFPQHIKALIPFVF 269
           RSYATRKWYVSKFEDFPQHIKALIPFVF
Sbjct: 247 RSYATRKWYVSKFEDFPQHIKALIPFVF 274

[Close]

BLAST of Bhi07G000610 vs. NCBI nr
Match: XP_023518338.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 448.0 bits (1151), Expect = 2.3e-122
Identity = 228/269 (84.76%), Postives = 246/269 (91.45%), Query Frame = 0


Query: 1   MDILSAFLFPSPPSLFVNVMTVVSSAVVAVIGLSEARGT-HLQYSKFWNTNNSATKSSNS 60
           MD +SA LF  PPSL VNVMTVVSS VVAVIGLSEARGT HLQYSKFWN NNSATKSSN 
Sbjct: 4   MDTISALLFRLPPSLLVNVMTVVSSVVVAVIGLSEARGTAHLQYSKFWNANNSATKSSNP 63

Query: 61  EQLRISSRLGMLLLYTPAALAGAASFWFFPDDDWRIVLLKSALTLHFFKRDFEVLFVHKY 120
           E+ +ISSR GMLLLY PAALAGA SFW FP+DD RI+LLKSALTLHFFKR+FEVLF+HKY
Sbjct: 64  EKFKISSRSGMLLLYAPAALAGAVSFWIFPNDDLRILLLKSALTLHFFKRNFEVLFIHKY 123

Query: 121 SGYMILDSAVIISLSYFSSTALMIYTQQLSKALVEPSIDLKNIGIAVFVIGIIGNMYHHY 180
           SG M LDSA+IISLSYFSSTALMIY Q+LS+ L EPSIDLK IGI++F+IGIIGN YHHY
Sbjct: 124 SGKMALDSALIISLSYFSSTALMIYAQELSRTLPEPSIDLKFIGISLFLIGIIGNFYHHY 183

Query: 181 ILSQTRKKGETGYKIPKGGLFSIVICPHYLFEITVYFGFALISQTLYSLCFAIATAIYLG 240
           ILSQTRKKGETGYKIPKGGLF +VICPHYLFEITVYFGFALISQT+YSLCFAIATA+YLG
Sbjct: 184 ILSQTRKKGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAMYLG 243

Query: 241 GRSYATRKWYVSKFEDFPQHIKALIPFVF 269
           GRSYATRKWY+SKFEDFPQHIKAL+PFVF
Sbjct: 244 GRSYATRKWYLSKFEDFPQHIKALVPFVF 272

[Close]

BLAST of Bhi07G000610 vs. NCBI nr
Match: XP_022956834.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata])

HSP 1 Score: 447.6 bits (1150), Expect = 3.0e-122
Identity = 229/269 (85.13%), Postives = 245/269 (91.08%), Query Frame = 0


Query: 1   MDILSAFLFPSPPSLFVNVMTVVSSAVVAVIGLSEARGT-HLQYSKFWNTNNSATKSSNS 60
           MD +SA LF  PPSL VNVMTVVSS VVAVIGLSEARGT HLQYSKFWNTNNSATKSSN 
Sbjct: 4   MDTISALLFRPPPSLLVNVMTVVSSVVVAVIGLSEARGTAHLQYSKFWNTNNSATKSSNL 63

Query: 61  EQLRISSRLGMLLLYTPAALAGAASFWFFPDDDWRIVLLKSALTLHFFKRDFEVLFVHKY 120
           E+ +ISSR GMLLLY PAALAGA SFW FP+DD RI+LLKSALTLHF KR+FEVLF+HKY
Sbjct: 64  EKFKISSRSGMLLLYAPAALAGAVSFWIFPNDDLRILLLKSALTLHFVKRNFEVLFIHKY 123

Query: 121 SGYMILDSAVIISLSYFSSTALMIYTQQLSKALVEPSIDLKNIGIAVFVIGIIGNMYHHY 180
           SG M LDSA+IISLSYFSSTALMIY Q+LS+ L EPSIDLK IGIA+F+IGIIGN YHHY
Sbjct: 124 SGKMALDSALIISLSYFSSTALMIYAQELSRTLPEPSIDLKFIGIALFLIGIIGNFYHHY 183

Query: 181 ILSQTRKKGETGYKIPKGGLFSIVICPHYLFEITVYFGFALISQTLYSLCFAIATAIYLG 240
           ILSQTRKKGETGYKIPKGGLF +VICPHYLFEITVYFGFALISQT+YSLCFAIATA+YLG
Sbjct: 184 ILSQTRKKGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAMYLG 243

Query: 241 GRSYATRKWYVSKFEDFPQHIKALIPFVF 269
           GRSYATRKWYVSKFEDFP HIKAL+PFVF
Sbjct: 244 GRSYATRKWYVSKFEDFPHHIKALVPFVF 272

[Close]

BLAST of Bhi07G000610 vs. NCBI nr
Match: XP_022984712.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucurbita maxima])

HSP 1 Score: 440.3 bits (1131), Expect = 4.8e-120
Identity = 224/269 (83.27%), Postives = 244/269 (90.71%), Query Frame = 0


Query: 1   MDILSAFLFPSPPSLFVNVMTVVSSAVVAVIGLSEARGT-HLQYSKFWNTNNSATKSSNS 60
           MD +S  LF  PPSL VNVMTVVSS VVAVIGLSEA+GT HLQYSKFWNTNNSATKSS+ 
Sbjct: 4   MDTISTLLFRPPPSLLVNVMTVVSSVVVAVIGLSEAKGTAHLQYSKFWNTNNSATKSSSP 63

Query: 61  EQLRISSRLGMLLLYTPAALAGAASFWFFPDDDWRIVLLKSALTLHFFKRDFEVLFVHKY 120
           E+ +ISSR GMLLLY PAALAGA SFW FP++D RI+LLKSALTLHF KR+FEVLF+HKY
Sbjct: 64  EKFKISSRSGMLLLYAPAALAGAVSFWIFPNEDLRILLLKSALTLHFVKRNFEVLFIHKY 123

Query: 121 SGYMILDSAVIISLSYFSSTALMIYTQQLSKALVEPSIDLKNIGIAVFVIGIIGNMYHHY 180
           SG M LDSA+IISLSYFSSTALMI+ Q+LS+ L EPSIDLK IGIA+F+IGIIGN YHHY
Sbjct: 124 SGKMALDSALIISLSYFSSTALMIFAQELSRTLPEPSIDLKFIGIALFLIGIIGNFYHHY 183

Query: 181 ILSQTRKKGETGYKIPKGGLFSIVICPHYLFEITVYFGFALISQTLYSLCFAIATAIYLG 240
           ILSQTRKKGETGYKIPKGGLF +VICPHYLFEITVYFGFALISQT+YSLCFAIATA+YLG
Sbjct: 184 ILSQTRKKGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAMYLG 243

Query: 241 GRSYATRKWYVSKFEDFPQHIKALIPFVF 269
           GRSYATRKWYVSKFEDFP HIKAL+PFVF
Sbjct: 244 GRSYATRKWYVSKFEDFPHHIKALVPFVF 272

The following BLAST results are available for this feature:

BLAST of Bhi07G000610 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q28891\|S5A1_MACFA	1.2e-18	42.06	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1...	[more]
sp\|O18765\|S5A2_PIG	1.2e-18	43.52	3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Sus scrofa OX=9823 GN=SRD5A2 PE=2 SV=...	[more]
sp\|Q68FF9\|S5A1_MOUSE	2.8e-18	43.81	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 ...	[more]
sp\|P18405\|S5A1_HUMAN	3.6e-18	41.12	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 S...	[more]
sp\|P24008\|S5A1_RAT	3.6e-18	44.76	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 ...	[more]

BLAST of Bhi07G000610 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G16010.1	1.6e-88	61.34	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	8.7e-15	37.04	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT3G55360.1	3.8e-10	32.35	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT1G72590.1	4.2e-09	35.04	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.1	5.5e-09	26.36	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

BLAST of Bhi07G000610 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BGE8\|A0A1S3BGE8_CUCME	3.1e-131	90.67	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
tr\|A0A0A0KRA7\|A0A0A0KRA7_CUCSA	7.1e-128	88.06	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G612890 PE=4 SV=1	[more]
tr\|A0A1S3BFS0\|A0A1S3BFS0_CUCME	7.1e-104	72.01	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
tr\|A0A0A0KWK8\|A0A0A0KWK8_CUCSA	6.6e-102	70.26	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G612900 PE=4 SV=1	[more]
tr\|A0A1S3BGK3\|A0A1S3BGK3_CUCME	2.1e-100	69.89	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo OX=3656 GN=LOC10348...	[more]

BLAST of Bhi07G000610 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008446723.1	4.6e-131	90.67	PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucumis melo]	[more]
XP_011655823.1	1.1e-127	88.06	PREDICTED: steroid 5-alpha-reductase DET2 [Cucumis sativus] >KGN52160.1 hypothet...	[more]
XP_023518338.1	2.3e-122	84.76	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022956834.1	3.0e-122	85.13	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata]	[more]
XP_022984712.1	4.8e-120	83.27	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucurbita maxima]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane
GO:0005737	cytoplasm

Vocabulary: **Biological Process**
Term	Definition
GO:0006629	lipid metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors

Vocabulary: **INTERPRO**
Term	Definition
IPR039357	SRD5A/TECR
IPR001104	3-oxo-5_a-steroid_4-DH_C

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi07M000610	Bhi07M000610	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 158..268 e-value: 5.4E-31 score: 107.5
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 163..243 score: 26.501
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 10..268
None	No IPR available	PANTHER	PTHR10556:SF35	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEIN	coord: 10..268

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi07G000610	Cucumber (Gy14) v1	cgywgoB583
Bhi07G000610	Cucumber (Gy14) v2	cgybwgoB477
Bhi07G000610	Cucurbita maxima (Rimu)	cmawgoB0544
Bhi07G000610	Cucurbita maxima (Rimu)	cmawgoB0903
Bhi07G000610	Cucurbita moschata (Rifu)	cmowgoB0540
Bhi07G000610	Cucurbita moschata (Rifu)	cmowgoB0904
Bhi07G000610	Cucurbita pepo (Zucchini)	cpewgoB0055
Bhi07G000610	Cucurbita pepo (Zucchini)	cpewgoB0569
Bhi07G000610	Wild cucumber (PI 183967)	cpiwgoB506
Bhi07G000610	Cucumber (Chinese Long) v3	cucwgoB513
Bhi07G000610	Cucumber (Chinese Long) v2	cuwgoB501
Bhi07G000610	Bottle gourd (USVL1VR-Ls)	lsiwgoB387
Bhi07G000610	Melon (DHL92) v3.6.1	medwgoB081
Bhi07G000610	Melon (DHL92) v3.5.1	mewgoB081
Bhi07G000610	Watermelon (Charleston Gray)	wcgwgoB469
Bhi07G000610	Watermelon (97103) v2	wgowmbB532
Bhi07G000610	Watermelon (97103) v1	wgowmB552
Bhi07G000610	Silver-seed gourd	carwgoB0979
Bhi07G000610	Silver-seed gourd	carwgoB1236