Bhi07G000258 (gene) Wax gourd

BLAST of Bhi07G000258 vs. Swiss-Prot
Match: sp|Q84WS0|SBT11_ARATH (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana OX=3702 GN=SBTI1.1 PE=1 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 1.1e-224
Identity = 394/714 (55.18%), Postives = 511/714 (71.57%), Query Frame = 0

Query: 60  SLDNDNNEEEASTAAEILYVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTT 119
           SL  +N  ++  +  EI Y+Y+ A+SGF+A L+   L ++    GF++A P++LL LHTT
Sbjct: 63  SLQTENINDDDFSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTT 122

Query: 120 HSPQFLGLKRGRGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGP 179
           +S +FLGL+ G GLWN ++L+SD+IIGL+DTGI PEH+SF+D  ++PVP++W+G C  G 
Sbjct: 123 YSHEFLGLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGT 182

Query: 180 KFSPSNCNKKLIGARAFIQGYEAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRAS 239
            FS S CNKK+IGA AF +GYE++VG++NET  FRS RD+ GHGTHTASTAAG+ V +A+
Sbjct: 183 NFSSSECNKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKAN 242

Query: 240 FYNQALGAATGMRFTSRIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLGGGSGIF 299
           ++ QA G A+GMRFTSRIAAYK CW  GCAS D++AA+D AI DGVDV+S+SLGG S  F
Sbjct: 243 YFGQAKGLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPF 302

Query: 300 YSDEIAIAAFGAIQQGVFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGN 359
           Y D IAIA FGA+Q+ +FVSCSAGNSGP  STV N APW+MTVAASYTDRTFP  V++GN
Sbjct: 303 YVDPIAIAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGN 362

Query: 360 GKVFEGSSLYFGKNINEIPLVYNNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGTNSRT 419
            K   GSSLY GK++  +PL +N TAG+   +  C   SL   +V+GKIV+C RG + RT
Sbjct: 363 RKSLVGSSLYKGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASGRT 422

Query: 420 AKGEQVKLAGGAGMILINTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQAKAS 479
           AKGE+VK +GGA M+L++T+ EGEEL+AD HVLPA ++G S  K +++Y+A + + A AS
Sbjct: 423 AKGEEVKRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAAN-ATAS 482

Query: 480 ITFKGTKYGSQAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKR 539
           + F+GT YG+ AP VAAFSSRGP                 NILA W P  SPS L SD R
Sbjct: 483 VRFRGTAYGATAPMVAAFSSRGPXXXXXXXXXXXXXXXXXNILAGWSPFSSPSLLRSDPR 542

Query: 540 RVLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDVSRA 599
           RV FNIISGTSM+CPH+SG+AAL+KS H DWSPA IKSA+MTTA +TDN+N  I D   A
Sbjct: 543 RVQFNIISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAA 602

Query: 600 SG-GPADPYAFGSGHVDPEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGKFTCS 659
                A  +AFG+G+VDP +A +PGLVYD +  DY+NYLCSL Y S +I L S   +TC+
Sbjct: 603 GAESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCA 662

Query: 660 SKRKFLQPGDLNYPSFSLFMKKKAKNVTITFKRTVTNVGIPRSDYTAKINNPEGIRVIVK 719
           S    L PGDLNYPSF++ +   A   T+ +KRTVTNVG P  +Y   +  P+G++V V+
Sbjct: 663 SNAVVLSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVE 722

Query: 720 PEKLSFVRLGGKLSYKVSFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVTWQ 773
           P+ L F +   +LSY V++ A   R +    SFG LVW   KY VRSPIAVTW+
Sbjct: 723 PKVLKFQKARERLSYTVTYDAEASRNS-SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of Bhi07G000258 vs. Swiss-Prot
Match: sp|Q9LUM3|SBT15_ARATH (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 684.1 bits (1764), Expect = 1.8e-195
Identity = 387/765 (50.59%), Postives = 488/765 (63.79%), Query Frame = 0

Query: 28  SYIIHMD-TSKMATANNPEQWYTVMIDSVNELASLDNDNNEEEASTAAEILYVYKTAISG 87
           +YI+H+D  +K +       WYT      + LASL         S+   I++ Y T   G
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYT------SSLASL--------TSSPPSIIHTYDTVFHG 86

Query: 88  FAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTTHSPQFLGLKR--GRGLWNSSNLASDII 147
           F+A+L+++    L   P  ++  P ++  LHTT SP+FLGL+     GL   S+  SD++
Sbjct: 87  FSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLV 146

Query: 148 IGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGPKFSPSNCNKKLIGARAFIQGYEAVV 207
           IG++DTG+WPE  SF D+GL PVP KWKG C A   F  S CN+KL+GAR F  GYEA  
Sbjct: 147 IGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATN 206

Query: 208 GRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRASFYNQALGAATGMRFTSRIAAYKVCW 267
           G++NET  FRSPRDSDGHGTHTAS +AG +V  AS    A G A GM   +R+AAYKVCW
Sbjct: 207 GKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCW 266

Query: 268 PEGCASADILAAMDHAIADGVDVLSISLGGGSGIFYSDEIAIAAFGAIQQGVFVSCSAGN 327
             GC  +DILAA D A+ADGVDV+S+S+GG    +Y D IAI AFGAI +G+FVS SAGN
Sbjct: 267 NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGN 326

Query: 328 SGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGNGKVFEGSSLYFGKNINE---IPLVY 387
            GP   TV NVAPW+ TV A   DR FP  VKLGNGK+  G S+Y G  ++     PLVY
Sbjct: 327 GGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVY 386

Query: 388 NNT--AGDGEESNVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQVKLAGGAGMILINTQ 447
             +   GDG  S++C  GSL P++VKGKIV+C+RG NSR  KGE V+  GG GMI+ N  
Sbjct: 387 GGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGV 446

Query: 448 LEGEELIADSHVLPATAVGASASKAIIDYIA------SSKHQAKASITFKGTKYGSQ-AP 507
            +GE L+AD HVLPAT+VGAS    I  YI+      SSKH   A+I FKGT+ G + AP
Sbjct: 447 FDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPT-ATIVFKGTRLGIRPAP 506

Query: 508 RVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMS 567
            VA+FS+RGP+   P ++KPD+ APG+NILAAWP  + PS + SD RR  FNI+SGTSM+
Sbjct: 507 VVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMA 566

Query: 568 CPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDVSRASGGPADPYAFGSG 627
           CPHVSGLAALLK+AH DWSPAAI+SAL+TTAY  DN    + D S  +G  +    +GSG
Sbjct: 567 CPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDES--TGNTSSVMDYGSG 626

Query: 628 HVDPEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGKFTCSSKRKFLQPGDLNYP 687
           HV P KA +PGLVYDI   DYIN+LC+  Y    I  ++R +  C   R+    G+LNYP
Sbjct: 627 HVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYP 686

Query: 688 SFS-LFMKKKAKNVTITFKRTVTNVGIPRSDYTAKINNPEGIRVIVKPEKLSFVRLGGKL 747
           SFS +F +     ++  F RTVTNVG   S Y  KI  P G  V V+PEKLSF R+G KL
Sbjct: 687 SFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKL 746

Query: 748 SY----KVSFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVTWQ 773
           S+    K + V L    T  +   G +VW  GK  V SP+ VT Q
Sbjct: 747 SFVVRVKTTEVKLSPGAT--NVETGHIVWSDGKRNVTSPLVVTLQ 772

BLAST of Bhi07G000258 vs. Swiss-Prot
Match: sp|Q9FLI4|SBT13_ARATH (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 680.6 bits (1755), Expect = 2.0e-194
Identity = 370/777 (47.62%), Postives = 493/777 (63.45%), Query Frame = 0

Query: 6   MLMFLSITIAIL--ATSSAAVDQQSYIIHMDTSKMATA-NNPEQWYTVMIDSVNELASLD 65
           + + LSI +  L   T++    +++Y+IHMD S M     N  QWY+  I+SV +  S +
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE 71

Query: 66  NDNNEEEASTAAEILYVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTTHSP 125
            + N         ILY Y+TA  G AA+L+ +    L +  G +A  P    +LHTT SP
Sbjct: 72  EEGNNN------RILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSP 131

Query: 126 QFLGLKR--GRGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGPK 185
            FLGL+R     +W       D+++G+LDTGIWPE  SF D G+SPVP  W+G C+ G +
Sbjct: 132 TFLGLERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKR 191

Query: 186 FSPSNCNKKLIGARAFIQGYEAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRASF 245
           F   NCN+K++GAR F +GYEA  G+++E   ++SPRD DGHGTHTA+T AG+ V  A+ 
Sbjct: 192 FLKRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANL 251

Query: 246 YNQALGAATGMRFTSRIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLGGGSGIFY 305
           +  A G A GM   +R+AAYKVCW  GC S+DIL+A+D A+ADGV VLSISLGGG   + 
Sbjct: 252 FGFAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYS 311

Query: 306 SDEIAIAAFGAIQQGVFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGNG 365
            D ++IA FGA++ GVFVSCSAGN GP   ++ NV+PWI TV AS  DR FP TVK+G  
Sbjct: 312 RDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTM 371

Query: 366 KVFEGSSLYFGKNI----NEIPLVY-NNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGT 425
           + F+G SLY G+ +     + PLVY    A   + ++ C  G+L    V GKIV+C+RG 
Sbjct: 372 RTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGV 431

Query: 426 NSRTAKGEQVKLAGGAGMILINTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQ 485
             R  KG+ VK AGG GM+L NT   GEEL+ADSH+LPA AVG    K I  Y  +SK +
Sbjct: 432 TPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSK-K 491

Query: 486 AKASITFKGTKYG-SQAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSEL 545
           A AS+   GT+ G   +P VAAFSSRGP+F    ++KPD+ APGVNILAAW   ++PS L
Sbjct: 492 ATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSL 551

Query: 546 ESDKRRVLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLIS 605
            SD RRV FNI+SGTSMSCPHVSG+AAL+KS H DWSPAAIKSALMTTAYV DN    ++
Sbjct: 552 SSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLT 611

Query: 606 DVSRASGGPADPYAFGSGHVDPEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGK 665
           D S A+  P+ PY  G+GH+DP +A++PGLVYDI PQ+Y  +LC+   + +Q+ + ++  
Sbjct: 612 DASGAA--PSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHS 671

Query: 666 FTCSSKRKFLQPGDLNYPSFSLFMKKKAKNVTITFKRTVTNVGIPRSDYTAKINNPEGIR 725
                      PG+LNYP+ S    +      +T +RTVTNVG   S Y   ++  +G  
Sbjct: 672 NRTCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGAS 731

Query: 726 VIVKPEKLSFVRLGGKLSYKVSFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVTW 772
           V V+P+ L+F     KLSY V+F     R  +    FG LVW+S  + VRSP+ +TW
Sbjct: 732 VTVQPKTLNFTSKHQKLSYTVTF---RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Bhi07G000258 vs. Swiss-Prot
Match: sp|O65351|SBT17_ARATH (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 678.3 bits (1749), Expect = 9.9e-194
Identity = 365/769 (47.46%), Postives = 493/769 (64.11%), Query Frame = 0

Query: 9   FLSITIAILATSSAAVDQQSYIIHMDTSKMATA-NNPEQWYTVMIDSVNELASLDNDNNE 68
           FL + +     SS++ DQ +YI+HM  S+M ++ +    WY               D++ 
Sbjct: 12  FLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWY---------------DSSL 71

Query: 69  EEASTAAEILYVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTTHSPQFLGL 128
              S +AE+LY Y+ AI GF+ +L+ +   SL   PG ++  P    +LHTT +P FLGL
Sbjct: 72  RSISDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGL 131

Query: 129 -KRGRGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGPKFSPSNC 188
            +    L+  +   SD+++G+LDTG+WPE  S+ D+G  P+P+ WKG C+AG  F+ S C
Sbjct: 132 DEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLC 191

Query: 189 NKKLIGARAFIQGYEAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRASFYNQALG 248
           N+KLIGAR F +GYE+ +G ++E+   RSPRD DGHGTHT+STAAG+ V  AS    A G
Sbjct: 192 NRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASG 251

Query: 249 AATGMRFTSRIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLGGGSGIFYSDEIAI 308
            A GM   +R+A YKVCW  GC S+DILAA+D AIAD V+VLS+SLGGG   +Y D +AI
Sbjct: 252 TARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAI 311

Query: 309 AAFGAIQQGVFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGNGKVFEGS 368
            AF A+++G+ VSCSAGN+GP  S++ NVAPWI TV A   DR FP    LGNGK F G 
Sbjct: 312 GAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGV 371

Query: 369 SLYFGKNINE--IPLVYNNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQ 428
           SL+ G+ + +  +P +Y   A +    N+C  G+L+P  VKGKIV+C+RG N+R  KG+ 
Sbjct: 372 SLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDV 431

Query: 429 VKLAGGAGMILINTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQAKASITFKG 488
           VK AGG GMIL NT   GEEL+AD+H+LPAT VG  A   I  Y+ +  +   ASI+  G
Sbjct: 432 VKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPT-ASISILG 491

Query: 489 TKYG-SQAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLF 548
           T  G   +P VAAFSSRGP+   P ++KPD+ APGVNILAAW     P+ L SD RRV F
Sbjct: 492 TVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEF 551

Query: 549 NIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDVSRASGGP 608
           NIISGTSMSCPHVSGLAALLKS H +WSPAAI+SALMTTAY T      + D+  A+G P
Sbjct: 552 NIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI--ATGKP 611

Query: 609 ADPYAFGSGHVDPEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGKFTCSSKRKF 668
           + P+  G+GHV P  A+NPGL+YD+  +DY+ +LC+L Y S QI  VSR  +TC   + +
Sbjct: 612 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY 671

Query: 669 LQPGDLNYPSFSLFMKKKAKNVTITFKRTVTNVGIPRSDYTAKI-NNPEGIRVIVKPEKL 728
               DLNYPSF++ +          + RTVT+VG     Y+ K+ +   G+++ V+P  L
Sbjct: 672 -SVADLNYPSFAVNVDGVG---AYKYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVL 731

Query: 729 SFVRLGGKLSYKVSFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVTW 772
           +F     K SY V+F     + +  + SFGS+ W  GK+ V SP+A++W
Sbjct: 732 NFKEANEKKSYTVTFTVDSSKPSGSN-SFGSIEWSDGKHVVGSPVAISW 756

BLAST of Bhi07G000258 vs. Swiss-Prot
Match: sp|Q9LVJ1|SBT14_ARATH (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 2.7e-191
Identity = 365/768 (47.53%), Postives = 493/768 (64.19%), Query Frame = 0

Query: 19  TSSAAVDQQSYIIHMDTS-KMATANNPEQWYTVMIDSVNELASLDNDNNEEEASTAAEIL 78
           +SS++   +SYI+H+  S K +  ++   W+  ++ S+              +   A +L
Sbjct: 23  SSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSL------------PSSPQPATLL 82

Query: 79  YVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTTHSPQFLGLKRGRGLWNSS 138
           Y Y  A+ GF+A+LS     +L + P  ++  P++  ++HTTH+P FLG  +  GLW++S
Sbjct: 83  YSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNS 142

Query: 139 NLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGPKFSPSNCNKKLIGARAFI 198
           N   D+I+G+LDTGIWPEH SF D GL P+P+ WKG C+ GP F  S+CN+KLIGARAF 
Sbjct: 143 NYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFY 202

Query: 199 QGY--EAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRASFYNQALGAATGMRFTS 258
           +GY  +    + +     RSPRD++GHGTHTASTAAG+ V  AS Y  A G ATGM   +
Sbjct: 203 RGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKA 262

Query: 259 RIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLG--GGSGIFYSDEIAIAAFGAIQ 318
           RIAAYK+CW  GC  +DILAAMD A+ADGV V+S+S+G  G +  +++D IAI AFGA +
Sbjct: 263 RIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATR 322

Query: 319 QGVFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGNGKVFEGSSLYFGKN 378
            G+ VSCSAGNSGP   T  N+APWI+TV AS  DR F      G+GKVF G+SLY G++
Sbjct: 323 HGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGES 382

Query: 379 I--NEIPLVYNNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQVKLAGGA 438
           +  +++ LVY+   G    S +C  G L  S+V+GKIV+C+RG N+R  KG  VKLAGGA
Sbjct: 383 LPDSQLSLVYSGDCG----SRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGA 442

Query: 439 GMILINTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQAKASITFKGTKYGSQ- 498
           GMIL NT   GEEL ADSH++PAT VGA A   I DYI +S     A I+F GT  G   
Sbjct: 443 GMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTS-DSPTAKISFLGTLIGPSP 502

Query: 499 -APRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGT 558
            +PRVAAFSSRGP+   P ++KPD+ APGVNILA W  +V P++L+ D RRV FNIISGT
Sbjct: 503 PSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGT 562

Query: 559 SMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDVSRASGGPADPYAF 618
           SMSCPHVSGLAALL+ AH DWSPAAIKSAL+TTAY  +N    I D+  A+G  ++ +  
Sbjct: 563 SMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDL--ATGKSSNSFIH 622

Query: 619 GSGHVDPEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGK--FTCSSKRKFLQPG 678
           G+GHVDP KA NPGLVYDI  ++Y+ +LC++ Y    I +  +    +      K    G
Sbjct: 623 GAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAG 682

Query: 679 DLNYPSFSLFMKKKAKNVTITFKRTVTNVGI-PRSDYTAKINNPEGIRVIVKPEKLSFVR 738
           DLNYPSFS+      +   + +KR V NVG    + Y   + +P  + + V P KL+F +
Sbjct: 683 DLNYPSFSVVFASTGE--VVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSK 742

Query: 739 LGGKLSYKVSFVAL---GKRETLDDFSFGSLVWQSGKYAVRSPIAVTW 772
               L Y+V+F ++   G   ++    FGS+ W  G++ V+SP+AV W
Sbjct: 743 EKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Bhi07G000258 vs. TAIR10
Match: AT1G01900.1 (subtilase family protein)

HSP 1 Score: 781.2 bits (2016), Expect = 6.0e-226
Identity = 394/714 (55.18%), Postives = 511/714 (71.57%), Query Frame = 0

Query: 60  SLDNDNNEEEASTAAEILYVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTT 119
           SL  +N  ++  +  EI Y+Y+ A+SGF+A L+   L ++    GF++A P++LL LHTT
Sbjct: 63  SLQTENINDDDFSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTT 122

Query: 120 HSPQFLGLKRGRGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGP 179
           +S +FLGL+ G GLWN ++L+SD+IIGL+DTGI PEH+SF+D  ++PVP++W+G C  G 
Sbjct: 123 YSHEFLGLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGT 182

Query: 180 KFSPSNCNKKLIGARAFIQGYEAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRAS 239
            FS S CNKK+IGA AF +GYE++VG++NET  FRS RD+ GHGTHTASTAAG+ V +A+
Sbjct: 183 NFSSSECNKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKAN 242

Query: 240 FYNQALGAATGMRFTSRIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLGGGSGIF 299
           ++ QA G A+GMRFTSRIAAYK CW  GCAS D++AA+D AI DGVDV+S+SLGG S  F
Sbjct: 243 YFGQAKGLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPF 302

Query: 300 YSDEIAIAAFGAIQQGVFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGN 359
           Y D IAIA FGA+Q+ +FVSCSAGNSGP  STV N APW+MTVAASYTDRTFP  V++GN
Sbjct: 303 YVDPIAIAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGN 362

Query: 360 GKVFEGSSLYFGKNINEIPLVYNNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGTNSRT 419
            K   GSSLY GK++  +PL +N TAG+   +  C   SL   +V+GKIV+C RG + RT
Sbjct: 363 RKSLVGSSLYKGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASGRT 422

Query: 420 AKGEQVKLAGGAGMILINTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQAKAS 479
           AKGE+VK +GGA M+L++T+ EGEEL+AD HVLPA ++G S  K +++Y+A + + A AS
Sbjct: 423 AKGEEVKRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAAN-ATAS 482

Query: 480 ITFKGTKYGSQAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKR 539
           + F+GT YG+ AP VAAFSSRGP                 NILA W P  SPS L SD R
Sbjct: 483 VRFRGTAYGATAPMVAAFSSRGPXXXXXXXXXXXXXXXXXNILAGWSPFSSPSLLRSDPR 542

Query: 540 RVLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDVSRA 599
           RV FNIISGTSM+CPH+SG+AAL+KS H DWSPA IKSA+MTTA +TDN+N  I D   A
Sbjct: 543 RVQFNIISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAA 602

Query: 600 SG-GPADPYAFGSGHVDPEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGKFTCS 659
                A  +AFG+G+VDP +A +PGLVYD +  DY+NYLCSL Y S +I L S   +TC+
Sbjct: 603 GAESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCA 662

Query: 660 SKRKFLQPGDLNYPSFSLFMKKKAKNVTITFKRTVTNVGIPRSDYTAKINNPEGIRVIVK 719
           S    L PGDLNYPSF++ +   A   T+ +KRTVTNVG P  +Y   +  P+G++V V+
Sbjct: 663 SNAVVLSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVE 722

Query: 720 PEKLSFVRLGGKLSYKVSFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVTWQ 773
           P+ L F +   +LSY V++ A   R +    SFG LVW   KY VRSPIAVTW+
Sbjct: 723 PKVLKFQKARERLSYTVTYDAEASRNS-SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of Bhi07G000258 vs. TAIR10
Match: AT3G14240.1 (Subtilase family protein)

HSP 1 Score: 684.1 bits (1764), Expect = 1.0e-196
Identity = 387/765 (50.59%), Postives = 488/765 (63.79%), Query Frame = 0

Query: 28  SYIIHMD-TSKMATANNPEQWYTVMIDSVNELASLDNDNNEEEASTAAEILYVYKTAISG 87
           +YI+H+D  +K +       WYT      + LASL         S+   I++ Y T   G
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYT------SSLASL--------TSSPPSIIHTYDTVFHG 86

Query: 88  FAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTTHSPQFLGLKR--GRGLWNSSNLASDII 147
           F+A+L+++    L   P  ++  P ++  LHTT SP+FLGL+     GL   S+  SD++
Sbjct: 87  FSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLV 146

Query: 148 IGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGPKFSPSNCNKKLIGARAFIQGYEAVV 207
           IG++DTG+WPE  SF D+GL PVP KWKG C A   F  S CN+KL+GAR F  GYEA  
Sbjct: 147 IGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATN 206

Query: 208 GRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRASFYNQALGAATGMRFTSRIAAYKVCW 267
           G++NET  FRSPRDSDGHGTHTAS +AG +V  AS    A G A GM   +R+AAYKVCW
Sbjct: 207 GKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCW 266

Query: 268 PEGCASADILAAMDHAIADGVDVLSISLGGGSGIFYSDEIAIAAFGAIQQGVFVSCSAGN 327
             GC  +DILAA D A+ADGVDV+S+S+GG    +Y D IAI AFGAI +G+FVS SAGN
Sbjct: 267 NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGN 326

Query: 328 SGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGNGKVFEGSSLYFGKNINE---IPLVY 387
            GP   TV NVAPW+ TV A   DR FP  VKLGNGK+  G S+Y G  ++     PLVY
Sbjct: 327 GGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVY 386

Query: 388 NNT--AGDGEESNVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQVKLAGGAGMILINTQ 447
             +   GDG  S++C  GSL P++VKGKIV+C+RG NSR  KGE V+  GG GMI+ N  
Sbjct: 387 GGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGV 446

Query: 448 LEGEELIADSHVLPATAVGASASKAIIDYIA------SSKHQAKASITFKGTKYGSQ-AP 507
            +GE L+AD HVLPAT+VGAS    I  YI+      SSKH   A+I FKGT+ G + AP
Sbjct: 447 FDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPT-ATIVFKGTRLGIRPAP 506

Query: 508 RVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMS 567
            VA+FS+RGP+   P ++KPD+ APG+NILAAWP  + PS + SD RR  FNI+SGTSM+
Sbjct: 507 VVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMA 566

Query: 568 CPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDVSRASGGPADPYAFGSG 627
           CPHVSGLAALLK+AH DWSPAAI+SAL+TTAY  DN    + D S  +G  +    +GSG
Sbjct: 567 CPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDES--TGNTSSVMDYGSG 626

Query: 628 HVDPEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGKFTCSSKRKFLQPGDLNYP 687
           HV P KA +PGLVYDI   DYIN+LC+  Y    I  ++R +  C   R+    G+LNYP
Sbjct: 627 HVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYP 686

Query: 688 SFS-LFMKKKAKNVTITFKRTVTNVGIPRSDYTAKINNPEGIRVIVKPEKLSFVRLGGKL 747
           SFS +F +     ++  F RTVTNVG   S Y  KI  P G  V V+PEKLSF R+G KL
Sbjct: 687 SFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKL 746

Query: 748 SY----KVSFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVTWQ 773
           S+    K + V L    T  +   G +VW  GK  V SP+ VT Q
Sbjct: 747 SFVVRVKTTEVKLSPGAT--NVETGHIVWSDGKRNVTSPLVVTLQ 772

BLAST of Bhi07G000258 vs. TAIR10
Match: AT5G51750.1 (subtilase 1.3)

HSP 1 Score: 680.6 bits (1755), Expect = 1.1e-195
Identity = 370/777 (47.62%), Postives = 493/777 (63.45%), Query Frame = 0

Query: 6   MLMFLSITIAIL--ATSSAAVDQQSYIIHMDTSKMATA-NNPEQWYTVMIDSVNELASLD 65
           + + LSI +  L   T++    +++Y+IHMD S M     N  QWY+  I+SV +  S +
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQE 71

Query: 66  NDNNEEEASTAAEILYVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTTHSP 125
            + N         ILY Y+TA  G AA+L+ +    L +  G +A  P    +LHTT SP
Sbjct: 72  EEGNNN------RILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSP 131

Query: 126 QFLGLKR--GRGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGPK 185
            FLGL+R     +W       D+++G+LDTGIWPE  SF D G+SPVP  W+G C+ G +
Sbjct: 132 TFLGLERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKR 191

Query: 186 FSPSNCNKKLIGARAFIQGYEAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRASF 245
           F   NCN+K++GAR F +GYEA  G+++E   ++SPRD DGHGTHTA+T AG+ V  A+ 
Sbjct: 192 FLKRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANL 251

Query: 246 YNQALGAATGMRFTSRIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLGGGSGIFY 305
           +  A G A GM   +R+AAYKVCW  GC S+DIL+A+D A+ADGV VLSISLGGG   + 
Sbjct: 252 FGFAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYS 311

Query: 306 SDEIAIAAFGAIQQGVFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGNG 365
            D ++IA FGA++ GVFVSCSAGN GP   ++ NV+PWI TV AS  DR FP TVK+G  
Sbjct: 312 RDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTM 371

Query: 366 KVFEGSSLYFGKNI----NEIPLVY-NNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGT 425
           + F+G SLY G+ +     + PLVY    A   + ++ C  G+L    V GKIV+C+RG 
Sbjct: 372 RTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGV 431

Query: 426 NSRTAKGEQVKLAGGAGMILINTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQ 485
             R  KG+ VK AGG GM+L NT   GEEL+ADSH+LPA AVG    K I  Y  +SK +
Sbjct: 432 TPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSK-K 491

Query: 486 AKASITFKGTKYG-SQAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSEL 545
           A AS+   GT+ G   +P VAAFSSRGP+F    ++KPD+ APGVNILAAW   ++PS L
Sbjct: 492 ATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSL 551

Query: 546 ESDKRRVLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLIS 605
            SD RRV FNI+SGTSMSCPHVSG+AAL+KS H DWSPAAIKSALMTTAYV DN    ++
Sbjct: 552 SSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLT 611

Query: 606 DVSRASGGPADPYAFGSGHVDPEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGK 665
           D S A+  P+ PY  G+GH+DP +A++PGLVYDI PQ+Y  +LC+   + +Q+ + ++  
Sbjct: 612 DASGAA--PSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHS 671

Query: 666 FTCSSKRKFLQPGDLNYPSFSLFMKKKAKNVTITFKRTVTNVGIPRSDYTAKINNPEGIR 725
                      PG+LNYP+ S    +      +T +RTVTNVG   S Y   ++  +G  
Sbjct: 672 NRTCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGAS 731

Query: 726 VIVKPEKLSFVRLGGKLSYKVSFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVTW 772
           V V+P+ L+F     KLSY V+F     R  +    FG LVW+S  + VRSP+ +TW
Sbjct: 732 VTVQPKTLNFTSKHQKLSYTVTF---RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Bhi07G000258 vs. TAIR10
Match: AT5G67360.1 (Subtilase family protein)

HSP 1 Score: 678.3 bits (1749), Expect = 5.5e-195
Identity = 365/769 (47.46%), Postives = 493/769 (64.11%), Query Frame = 0

Query: 9   FLSITIAILATSSAAVDQQSYIIHMDTSKMATA-NNPEQWYTVMIDSVNELASLDNDNNE 68
           FL + +     SS++ DQ +YI+HM  S+M ++ +    WY               D++ 
Sbjct: 12  FLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWY---------------DSSL 71

Query: 69  EEASTAAEILYVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTTHSPQFLGL 128
              S +AE+LY Y+ AI GF+ +L+ +   SL   PG ++  P    +LHTT +P FLGL
Sbjct: 72  RSISDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGL 131

Query: 129 -KRGRGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGPKFSPSNC 188
            +    L+  +   SD+++G+LDTG+WPE  S+ D+G  P+P+ WKG C+AG  F+ S C
Sbjct: 132 DEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLC 191

Query: 189 NKKLIGARAFIQGYEAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRASFYNQALG 248
           N+KLIGAR F +GYE+ +G ++E+   RSPRD DGHGTHT+STAAG+ V  AS    A G
Sbjct: 192 NRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASG 251

Query: 249 AATGMRFTSRIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLGGGSGIFYSDEIAI 308
            A GM   +R+A YKVCW  GC S+DILAA+D AIAD V+VLS+SLGGG   +Y D +AI
Sbjct: 252 TARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAI 311

Query: 309 AAFGAIQQGVFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGNGKVFEGS 368
            AF A+++G+ VSCSAGN+GP  S++ NVAPWI TV A   DR FP    LGNGK F G 
Sbjct: 312 GAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGV 371

Query: 369 SLYFGKNINE--IPLVYNNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQ 428
           SL+ G+ + +  +P +Y   A +    N+C  G+L+P  VKGKIV+C+RG N+R  KG+ 
Sbjct: 372 SLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDV 431

Query: 429 VKLAGGAGMILINTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQAKASITFKG 488
           VK AGG GMIL NT   GEEL+AD+H+LPAT VG  A   I  Y+ +  +   ASI+  G
Sbjct: 432 VKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPT-ASISILG 491

Query: 489 TKYG-SQAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLF 548
           T  G   +P VAAFSSRGP+   P ++KPD+ APGVNILAAW     P+ L SD RRV F
Sbjct: 492 TVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEF 551

Query: 549 NIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDVSRASGGP 608
           NIISGTSMSCPHVSGLAALLKS H +WSPAAI+SALMTTAY T      + D+  A+G P
Sbjct: 552 NIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDI--ATGKP 611

Query: 609 ADPYAFGSGHVDPEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGKFTCSSKRKF 668
           + P+  G+GHV P  A+NPGL+YD+  +DY+ +LC+L Y S QI  VSR  +TC   + +
Sbjct: 612 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY 671

Query: 669 LQPGDLNYPSFSLFMKKKAKNVTITFKRTVTNVGIPRSDYTAKI-NNPEGIRVIVKPEKL 728
               DLNYPSF++ +          + RTVT+VG     Y+ K+ +   G+++ V+P  L
Sbjct: 672 -SVADLNYPSFAVNVDGVG---AYKYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVL 731

Query: 729 SFVRLGGKLSYKVSFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVTW 772
           +F     K SY V+F     + +  + SFGS+ W  GK+ V SP+A++W
Sbjct: 732 NFKEANEKKSYTVTFTVDSSKPSGSN-SFGSIEWSDGKHVVGSPVAISW 756

BLAST of Bhi07G000258 vs. TAIR10
Match: AT3G14067.1 (Subtilase family protein)

HSP 1 Score: 670.2 bits (1728), Expect = 1.5e-192
Identity = 365/768 (47.53%), Postives = 493/768 (64.19%), Query Frame = 0

Query: 19  TSSAAVDQQSYIIHMDTS-KMATANNPEQWYTVMIDSVNELASLDNDNNEEEASTAAEIL 78
           +SS++   +SYI+H+  S K +  ++   W+  ++ S+              +   A +L
Sbjct: 23  SSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSL------------PSSPQPATLL 82

Query: 79  YVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTTHSPQFLGLKRGRGLWNSS 138
           Y Y  A+ GF+A+LS     +L + P  ++  P++  ++HTTH+P FLG  +  GLW++S
Sbjct: 83  YSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNS 142

Query: 139 NLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGPKFSPSNCNKKLIGARAFI 198
           N   D+I+G+LDTGIWPEH SF D GL P+P+ WKG C+ GP F  S+CN+KLIGARAF 
Sbjct: 143 NYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFY 202

Query: 199 QGY--EAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRASFYNQALGAATGMRFTS 258
           +GY  +    + +     RSPRD++GHGTHTASTAAG+ V  AS Y  A G ATGM   +
Sbjct: 203 RGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKA 262

Query: 259 RIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLG--GGSGIFYSDEIAIAAFGAIQ 318
           RIAAYK+CW  GC  +DILAAMD A+ADGV V+S+S+G  G +  +++D IAI AFGA +
Sbjct: 263 RIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATR 322

Query: 319 QGVFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGNGKVFEGSSLYFGKN 378
            G+ VSCSAGNSGP   T  N+APWI+TV AS  DR F      G+GKVF G+SLY G++
Sbjct: 323 HGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGES 382

Query: 379 I--NEIPLVYNNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQVKLAGGA 438
           +  +++ LVY+   G    S +C  G L  S+V+GKIV+C+RG N+R  KG  VKLAGGA
Sbjct: 383 LPDSQLSLVYSGDCG----SRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGA 442

Query: 439 GMILINTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQAKASITFKGTKYGSQ- 498
           GMIL NT   GEEL ADSH++PAT VGA A   I DYI +S     A I+F GT  G   
Sbjct: 443 GMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTS-DSPTAKISFLGTLIGPSP 502

Query: 499 -APRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGT 558
            +PRVAAFSSRGP+   P ++KPD+ APGVNILA W  +V P++L+ D RRV FNIISGT
Sbjct: 503 PSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGT 562

Query: 559 SMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDVSRASGGPADPYAF 618
           SMSCPHVSGLAALL+ AH DWSPAAIKSAL+TTAY  +N    I D+  A+G  ++ +  
Sbjct: 563 SMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDL--ATGKSSNSFIH 622

Query: 619 GSGHVDPEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGK--FTCSSKRKFLQPG 678
           G+GHVDP KA NPGLVYDI  ++Y+ +LC++ Y    I +  +    +      K    G
Sbjct: 623 GAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAG 682

Query: 679 DLNYPSFSLFMKKKAKNVTITFKRTVTNVGI-PRSDYTAKINNPEGIRVIVKPEKLSFVR 738
           DLNYPSFS+      +   + +KR V NVG    + Y   + +P  + + V P KL+F +
Sbjct: 683 DLNYPSFSVVFASTGE--VVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSK 742

Query: 739 LGGKLSYKVSFVAL---GKRETLDDFSFGSLVWQSGKYAVRSPIAVTW 772
               L Y+V+F ++   G   ++    FGS+ W  G++ V+SP+AV W
Sbjct: 743 EKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Bhi07G000258 vs. TrEMBL
Match: tr|A0A1S3BZC2|A0A1S3BZC2_CUCME (subtilisin-like protease SBT1.1 OS=Cucumis melo OX=3656 GN=LOC103495085 PE=3 SV=1)

HSP 1 Score: 1312.7 bits (3396), Expect = 0.0e+00
Identity = 658/772 (85.23%), Postives = 708/772 (91.71%), Query Frame = 0

Query: 1   MKIRQMLMFLSITIAILATSSAAVDQQSYIIHMDTSKMATANNPEQWYTVMIDSVNELAS 60
           M  +++L+FL IT  +L TS  A+DQQSYI+HMDT+KMAT  NPEQWYT +I SVN+L+S
Sbjct: 1   MGFKEVLLFLYIT--MLTTSIVAMDQQSYIVHMDTTKMAT-TNPEQWYTAIIHSVNKLSS 60

Query: 61  LDNDNNEEEASTAAEILYVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTTH 120
           LD +NNEE+A   AEILYVYKT ISGF AKLS+K+LH LSK+PGF+AA+PN+LLQLHTTH
Sbjct: 61  LDANNNEEQALNIAEILYVYKTVISGFCAKLSSKNLHFLSKVPGFIAASPNELLQLHTTH 120

Query: 121 SPQFLGLKRGRGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGPK 180
           SP+FLGL+RG GLWNSSNLASDIIIG+LDTGIWPEHISFQDK L PVP+KWKGICQ GP 
Sbjct: 121 SPKFLGLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKALPPVPSKWKGICQTGPS 180

Query: 181 FSPSNCNKKLIGARAFIQGYEAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRASF 240
           FS SNCNKKLIGAR FIQ YEA VGRLN TGTFRS RDSDGHGTHTASTAAGNFVNRASF
Sbjct: 181 FSLSNCNKKLIGARTFIQAYEAAVGRLNATGTFRSARDSDGHGTHTASTAAGNFVNRASF 240

Query: 241 YNQALGAATGMRFTSRIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLGGGSGIFY 300
           YNQ +G ATGMRFTSRIAAYKVCWPEGCASADILAAMDHA+ADGVDVLSISLGGGS I Y
Sbjct: 241 YNQGMGVATGMRFTSRIAAYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIY 300

Query: 301 SDEIAIAAFGAIQQGVFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGNG 360
           SD+IAIAAFGAIQ+GVFVSCSAGNSGP+ISTVGNVAPW+MTVAASYTDRTFPTTV+LGNG
Sbjct: 301 SDQIAIAAFGAIQKGVFVSCSAGNSGPFISTVGNVAPWVMTVAASYTDRTFPTTVRLGNG 360

Query: 361 KVFEGSSLYFGKNINEIPLVYNNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGTNSRTA 420
            VFEGSSLYFGKN+ E PLVYNNTAGDG E+N CT GSL P+MVKGKI VCERGTNSRT 
Sbjct: 361 MVFEGSSLYFGKNLKETPLVYNNTAGDGRETNFCTPGSLDPTMVKGKIAVCERGTNSRTE 420

Query: 421 KGEQVKLAGGAGMILINTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQAKASI 480
           KGEQVKLAGGAGMILINT LEGE+L+ADSHVLPAT+VG SA K+I++YIASSK QAKASI
Sbjct: 421 KGEQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGVSAGKSILNYIASSKRQAKASI 480

Query: 481 TFKGTKYGSQAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRR 540
            FKGTKYGS+APRVAAFSSRGPSF+KPYVIKPDITAPGVNILAAWPPIVSPSELESDKRR
Sbjct: 481 MFKGTKYGSRAPRVAAFSSRGPSFYKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRR 540

Query: 541 VLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDVSRAS 600
           VLFNIISGTSMSCPHVSGLAALLKS HKDWSPAAIKSALMTTAYVTDNKNHLISDV RAS
Sbjct: 541 VLFNIISGTSMSCPHVSGLAALLKSVHKDWSPAAIKSALMTTAYVTDNKNHLISDVGRAS 600

Query: 601 GGPADPYAFGSGHVDPEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGKFTCSSK 660
           GGPADP+AFGSGHVDPEKAS+PGLVYDIAPQDYI YLCSLKYNS QIALVSRGKFTCSSK
Sbjct: 601 GGPADPFAFGSGHVDPEKASDPGLVYDIAPQDYITYLCSLKYNSTQIALVSRGKFTCSSK 660

Query: 661 RKFLQPGDLNYPSFSLFMKKKAKNVTITFKRTVTNVGIPRSDYTAKINNPEGIRVIVKPE 720
           R F QPGDLNYPSFS+FM KK KNV  TFKRTVTNVGIPRSDYT +I NP+GIR+IVKPE
Sbjct: 661 RTFFQPGDLNYPSFSVFM-KKGKNVNSTFKRTVTNVGIPRSDYTVRIYNPKGIRIIVKPE 720

Query: 721 KLSFVRLGGKLSYKVSFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVTWQ 773
           KLSFV+LG KLSYKVSFVALGKR++LDDFSFGSLVW SG Y VRSPIAVTWQ
Sbjct: 721 KLSFVKLGEKLSYKVSFVALGKRKSLDDFSFGSLVWHSGTYVVRSPIAVTWQ 768

BLAST of Bhi07G000258 vs. TrEMBL
Match: tr|A0A1S3C0J8|A0A1S3C0J8_CUCME (subtilisin-like protease SBT1.1 OS=Cucumis melo OX=3656 GN=LOC103495084 PE=4 SV=1)

HSP 1 Score: 1218.4 bits (3151), Expect = 0.0e+00
Identity = 618/757 (81.64%), Postives = 669/757 (88.38%), Query Frame = 0

Query: 16  ILATSSAAVDQQSYIIHMDTSKMATANNPEQWYTVMIDSVNELASLDNDNNEEEASTAAE 75
           +LA SSA VDQQ+YIIHMDT+KM T  NPEQWYT +IDSVNEL+SLD++   EEAS AAE
Sbjct: 12  MLAISSAVVDQQTYIIHMDTTKMVTP-NPEQWYTDIIDSVNELSSLDDN---EEASNAAE 71

Query: 76  ILYVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTTHSPQFLGLKRGRGLWN 135
           ILYVYKTA+SGFAAKL++K LHSLSKIPGFLAATPN+LLQLHTTHSPQFLGL+R  GLWN
Sbjct: 72  ILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDHGLWN 131

Query: 136 SSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGPKFSPSNCNKKLIGARA 195
            SNLASDIIIGLLDTGIWPEHISFQDKGLS VP KWKGICQ GP+FS SNCNKKLIGA A
Sbjct: 132 FSNLASDIIIGLLDTGIWPEHISFQDKGLSSVPLKWKGICQTGPRFSSSNCNKKLIGASA 191

Query: 196 FIQGYEAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRASFYNQALGAATGMRFTS 255
           +I+GYEA+VGRLNETGTFRSPRDSDGHGTHTASTAAG+ V+ ASFYNQ +G A+GMRFTS
Sbjct: 192 YIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGSIVDNASFYNQGMGVASGMRFTS 251

Query: 256 RIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLGGGSGIFYSDEIAIAAFGAIQQG 315
           RI AYKVCWP GCA+ADILAAMD A+ADGVDVLS+SLGGGS  FY D IAIAAFGAIQ+G
Sbjct: 252 RIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAIAAFGAIQKG 311

Query: 316 VFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGNGKVFEGSSLYFGKNIN 375
           VFVSCSAGNSGP  STVGN APWIMTVAASYTDRTFPTTVKLGNG+VFEGSSLY+GK+IN
Sbjct: 312 VFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSSLYYGKSIN 371

Query: 376 EIPLVYNNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQVKLAGGAGMIL 435
           E+PLVYNNTAGDG+E+NVC AGSL PSMVKGKIV+CERGT SRT KGEQVKLAGG GMIL
Sbjct: 372 ELPLVYNNTAGDGQETNVCIAGSLDPSMVKGKIVICERGTISRTEKGEQVKLAGGTGMIL 431

Query: 436 INTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQAKASITFKGTKYGSQAPRVA 495
           INTQ EGEEL AD HVLPAT +GASA KAI+DYIASSK QAKASI F+GTKYGSQAPRVA
Sbjct: 432 INTQFEGEELFADPHVLPATTLGASAGKAILDYIASSKTQAKASIVFEGTKYGSQAPRVA 491

Query: 496 AFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPH 555
           AFSSRGPS                NILAAWPPIVSPSEL SD RRV+FNIISGTSMSCPH
Sbjct: 492 AFSSRGPSLXXXXXXXXXXXXXXXNILAAWPPIVSPSELASDTRRVMFNIISGTSMSCPH 551

Query: 556 VSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDVSRASGGPADPYAFGSGHVD 615
           VSGLAALLKSAH DWSPAAIKSALMTTAYVTD+K  LISDV +A+G PA P+ FGSGHVD
Sbjct: 552 VSGLAALLKSAHNDWSPAAIKSALMTTAYVTDSKMSLISDVGQANGEPATPFTFGSGHVD 611

Query: 616 PEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGKFTCSSKRKFLQPGDLNYPSFS 675
           PEKAS+PGL+YDI PQDYINYLCSLKYNS+QIALVSRG  TCSSKR  ++PGDLNYPSFS
Sbjct: 612 PEKASDPGLIYDITPQDYINYLCSLKYNSSQIALVSRGNLTCSSKRTVVKPGDLNYPSFS 671

Query: 676 LFMKKKAKNVTITFKRTVTNVGIPRSDYTAKINNPEGIRVIVKPEKLSFVRLGGKLSYKV 735
           +FMKKKAK V+IT KRTVTNVGI RSDYT KINNP+G+ VIVKPEKLSF  LG +LSYKV
Sbjct: 672 VFMKKKAKKVSITLKRTVTNVGITRSDYTVKINNPKGVTVIVKPEKLSFGSLGEQLSYKV 731

Query: 736 SFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVTWQ 773
           SFV+LG +E LD FSFGSLVW SGKYAVRSPI VTWQ
Sbjct: 732 SFVSLGGKEALDKFSFGSLVWISGKYAVRSPIVVTWQ 764

BLAST of Bhi07G000258 vs. TrEMBL
Match: tr|V7AQQ3|V7AQQ3_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_010G088700g PE=3 SV=1)

HSP 1 Score: 943.7 bits (2438), Expect = 2.5e-271
Identity = 482/776 (62.11%), Postives = 598/776 (77.06%), Query Frame = 0

Query: 1   MKIRQMLMFLSITIAILATSSAAVDQQSYIIHMDTSKMATA----NNPEQWYTVMIDSVN 60
           M  R +L+FL+    ++  S A +++Q+YI+HMD +K+  +    +N + W+  +ID ++
Sbjct: 1   MIFRALLLFLAF---MVVKSVAVMEKQTYIVHMDKTKIEASVHSQDNIKPWFKSVIDFIS 60

Query: 61  ELASLDNDNNEEEASTAAEILYVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQL 120
           E ASL      EE   A ++LYVY+T++ GFAA+LS K L  L+++ GF+AA P++LL L
Sbjct: 61  E-ASL------EEEERAPQLLYVYETSLFGFAAQLSNKQLEYLNQVDGFVAAIPDELLTL 120

Query: 121 HTTHSPQFLGLKRGRGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQ 180
           HTT+SP FLGL+ G+GLW++SNLASD+IIG+LDTGIWPEH+SFQD GLS VP++WKG C+
Sbjct: 121 HTTYSPHFLGLQEGKGLWSASNLASDVIIGVLDTGIWPEHVSFQDTGLSKVPSRWKGACE 180

Query: 181 AGPKFSPSNCNKKLIGARAFIQGYEAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVN 240
           AG  FS S+CNKKL+GAR F+QGYE   GR+NET  +RS RD+ GHG+HTASTAAGN VN
Sbjct: 181 AGTNFSASSCNKKLVGARVFLQGYEKFAGRINETLDYRSARDAQGHGSHTASTAAGNTVN 240

Query: 241 RASFYNQALGAATGMRFTSRIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLGGGS 300
            ASF+  A G+ATGMR+TSRIAAYKVCW  GCA++DILAA+D A+ADGVDVLS+SLGG +
Sbjct: 241 NASFFGLASGSATGMRYTSRIAAYKVCWRLGCANSDILAAIDKAVADGVDVLSLSLGGIA 300

Query: 301 GIFYSDEIAIAAFGAIQQGVFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVK 360
             +Y+D IAIA+FGA Q+GVFVSCSAGNSGP  STVGNVAPWIMTVAASYTDR+FPT VK
Sbjct: 301 KPYYNDSIAIASFGATQKGVFVSCSAGNSGPSSSTVGNVAPWIMTVAASYTDRSFPTQVK 360

Query: 361 LGNGKVFEGSSLYFGKNINEIPLVYNNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGTN 420
           LGNGK F+GSSLY GK  N++PLVY N++     +  CT GSL P  VKGKIV CERG N
Sbjct: 361 LGNGKFFKGSSLYKGKQTNQLPLVYGNSSKAQRTAQYCTKGSLDPKFVKGKIVACERGIN 420

Query: 421 SRTAKGEQVKLAGGAGMILINTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQA 480
           SRT KGE+VK+AGGAGMIL+N++ +GEEL AD HVLP T++GASASK I  YI S K   
Sbjct: 421 SRTGKGEEVKMAGGAGMILLNSENQGEELFADPHVLPGTSLGASASKIIRSYIHSEK-AP 480

Query: 481 KASITFKGTKYGSQAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELES 540
            ASI+F GT YG  AP +AAFSSRGPS     VIKPD+TAPGVNILAAWPP  SPS L+S
Sbjct: 481 TASISFLGTAYGDPAPVMAAFSSRGPSAVGGDVIKPDVTAPGVNILAAWPPTTSPSMLKS 540

Query: 541 DKRRVLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDV 600
           DKR  L+NI+SGTSMSCPHVSG+AAL+KS HK WSPAAIKSALMTTA +++NK   I+D 
Sbjct: 541 DKRSALYNIVSGTSMSCPHVSGIAALIKSVHKGWSPAAIKSALMTTASISNNKGSAIADY 600

Query: 601 SRASGGPADPYAFGSGHVDPEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGKFT 660
              +   ADP+AFGSGHV+PE+AS+PGLVYDI  +DY+NYLCSLKY S+QIAL+S+G F 
Sbjct: 601 GSKNSAFADPFAFGSGHVNPERASDPGLVYDITTKDYLNYLCSLKYTSSQIALLSKGNFK 660

Query: 661 CSSKRKFLQPGDLNYPSFSLFMKKKAKNVTITFKRTVTNVGIPRSDYTAKINNPEGIRVI 720
           C +K+  L+ GDLNYPSF++     A N ++ +KR VTNVG P+  Y  K+  P+G+ V 
Sbjct: 661 C-AKKSALRAGDLNYPSFAVLFGTSALNASVRYKRIVTNVGNPKISYAVKVEEPKGVSVR 720

Query: 721 VKPEKLSFVRLGGKLSYKVSFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVTWQ 773
           V+P  +SF + G KLSYKVSFV+          SFGS+ W SGKYAVRSPIAVTWQ
Sbjct: 721 VEPTNISFRKTGEKLSYKVSFVSNENTTVSGSSSFGSITWVSGKYAVRSPIAVTWQ 764

BLAST of Bhi07G000258 vs. TrEMBL
Match: tr|A0A2P4MBZ9|A0A2P4MBZ9_QUESU (Subtilisin-like protease sbt1.1 OS=Quercus suber OX=58331 GN=CFP56_45295 PE=3 SV=1)

HSP 1 Score: 941.8 bits (2433), Expect = 9.7e-271
Identity = 481/774 (62.14%), Postives = 600/774 (77.52%), Query Frame = 0

Query: 7   LMFLSITIAILATSSAAVDQQSYIIHMDTSKMATANN----PEQWYTVMIDSVNELASLD 66
           L+FL+    +  TS A++++++YIIHMD +K+  +++     + WY +M+DS++E+ S  
Sbjct: 7   LLFLAF---MATTSIASMNKETYIIHMDKTKITASDHTLGIAKPWYELMMDSMHEILS-- 66

Query: 67  NDNNEEEASTAAEILYVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTTHSP 126
               EEE  +  E+LY+YKT ISGFAA+LSTK + SL ++ GFL+ATP+++L LH TH+P
Sbjct: 67  ---QEEEDMSPLELLYIYKTTISGFAAQLSTKQVQSLKRLNGFLSATPDEMLSLHITHTP 126

Query: 127 QFLGLKRGRGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGPKFS 186
           QFLGL  G GLWN+  LASD+IIG++DTGIWPEH+SF+D  +S VP++WKG C+ G KFS
Sbjct: 127 QFLGLHLGEGLWNAPILASDVIIGIVDTGIWPEHVSFKDSSISQVPSRWKGTCEEGTKFS 186

Query: 187 PSNCNKKLIGARAFIQGYEAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRASFYN 246
            +NCNKKLIGARAF +GYE +VGR+NET  +RSPRD +GHGTHTASTAAGN V+ A+F  
Sbjct: 187 VANCNKKLIGARAFYKGYETIVGRINETTDYRSPRDFNGHGTHTASTAAGNLVHNANFLG 246

Query: 247 QALGAATGMRFTSRIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLGGGSGIFYSD 306
            A G A+GM +TSRIAAYK CW  GC ++DILAA+D A+ADGVDVLS+SLGG S  +YSD
Sbjct: 247 MAKGLASGMSYTSRIAAYKACWRRGCTNSDILAAIDRAVADGVDVLSLSLGGFSKPYYSD 306

Query: 307 EIAIAAFGAIQQGVFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGNGKV 366
            IAIA+FGA+Q GVFVSCSAGNSGP+ S+V N APWIMTVAASY DR+FPTTVKLGNGK 
Sbjct: 307 NIAIASFGAVQNGVFVSCSAGNSGPFSSSVSNAAPWIMTVAASYIDRSFPTTVKLGNGKT 366

Query: 367 FEGSSLYFGKNINEIPLVYNNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGTNSRTAKG 426
           FEGSSLY G+   ++PLVY +TAG G+ +  C   SLVP +VKGKIV CE+G NSR  K 
Sbjct: 367 FEGSSLYSGERTKQLPLVYASTAG-GQGALFCLNDSLVPKLVKGKIVACEQGINSRAEKE 426

Query: 427 EQVKLAGGAGMILINTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQAKASITF 486
           EQVK AGGAGM+L+N ++ GEEL+ADSH LPAT++GASA KAI +Y+  +K+   ASI F
Sbjct: 427 EQVKNAGGAGMLLLNAEIYGEELLADSHTLPATSLGASAGKAIKNYLCLNKNPT-ASIMF 486

Query: 487 KGTKYGSQAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVL 546
           +GT YG+ AP +AAFSSRGPS     VIKPDITAPGVNILAAWPP   PS L++DKR +L
Sbjct: 487 RGTVYGNIAPVMAAFSSRGPSLVGQDVIKPDITAPGVNILAAWPPKTIPSLLKNDKRSIL 546

Query: 547 FNIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDVSRASGG 606
           FNIISGTSMSCPHVSGLAALLKS HKDWSPAAIKSALMTTAYV ++K   I+D++  S  
Sbjct: 547 FNIISGTSMSCPHVSGLAALLKSIHKDWSPAAIKSALMTTAYVLNSKRAPIADIASNSSK 606

Query: 607 PADPYAFGSGHVDPEKAS------NPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGKFT 666
            A P+AFGSGHV+P+ AS      +PGL+YDI  +DY+NYLCSL YNS QIAL+SRG FT
Sbjct: 607 AATPFAFGSGHVNPKCASDPGPGTDPGLIYDITSEDYLNYLCSLNYNSTQIALLSRGNFT 666

Query: 667 CSSKRKFLQPGDLNYPSFSLFMKKKAKNVTITFKRTVTNVGIPRSDYTAKINNPEGIRVI 726
           C +   FLQPGDLNYPSF++ + + A N ++T+KRTVTNVG P S Y  ++  P G+ V 
Sbjct: 667 CPN-NAFLQPGDLNYPSFAVLLDQNASNTSVTYKRTVTNVGTPMSTYVVQVYEPNGVSVT 726

Query: 727 VKPEKLSFVRLGGKLSYKVSFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVT 771
           V+P+ L F  LG KLSYKV F AL + ++  +FSFGSLVW+S  Y VRSPIAVT
Sbjct: 727 VEPKCLKFENLGEKLSYKVRFNALRRTKSSTNFSFGSLVWKSEFYTVRSPIAVT 769

BLAST of Bhi07G000258 vs. TrEMBL
Match: tr|B9IC48|B9IC48_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_014G074600v3 PE=3 SV=2)

HSP 1 Score: 941.8 bits (2433), Expect = 9.7e-271
Identity = 478/774 (61.76%), Postives = 588/774 (75.97%), Query Frame = 0

Query: 8   MFLSITIAILA-----TSSAAVDQQSYIIHMDTSKMAT----ANNPEQWYTVMIDSVNEL 67
           M   I++ +LA      S+A++D+Q+YIIHMD +KM        N  QWY  +IDS+ + 
Sbjct: 1   MIFRISLLLLAFMAAKASAASIDKQTYIIHMDKNKMPALYDFLGNSRQWYESVIDSITQF 60

Query: 68  ASLDNDNNEEEASTAAEILYVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQLHT 127
           +S   ++ EE  +   ++LY Y+T  SGFAAKLSTK + +LS++ GFL+A P+ +L LHT
Sbjct: 61  SS--QEHEEEHETGFPQLLYTYETVTSGFAAKLSTKQVEALSRVDGFLSAIPDGMLTLHT 120

Query: 128 THSPQFLGLKRGRGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAG 187
           TH+P+FLGL+ G+GLWN+ NLASD+I+G+LDTGIWPEH+SFQD G+S VP KWKG C++G
Sbjct: 121 THTPRFLGLQSGKGLWNAQNLASDVIVGILDTGIWPEHVSFQDSGMSAVPLKWKGKCESG 180

Query: 188 PKFSPSNCNKKLIGARAFIQGYEAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRA 247
            KFSPSNCNKKLIGARAF +GYE++VGR+NET  +RSPRDS GHGTHTA+TAAGN V+ A
Sbjct: 181 TKFSPSNCNKKLIGARAFFKGYESIVGRINETIDYRSPRDSQGHGTHTAATAAGNLVDEA 240

Query: 248 SFYNQALGAATGMRFTSRIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLGGGSGI 307
           SFY  A G+A GM++T+RIAAYKVCW  GC + D+LAA+D A+ADGVDVLS+SLGG +  
Sbjct: 241 SFYGLANGSAAGMKYTARIAAYKVCWTSGCTNTDLLAAIDQAVADGVDVLSLSLGGSAKP 300

Query: 308 FYSDEIAIAAFGAIQQGVFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLG 367
           FYSD +AIA+FGAIQ+GVFVSCSAGNSGP IS+V N APWIMTVAASYTDR FPTTVKLG
Sbjct: 301 FYSDSVAIASFGAIQKGVFVSCSAGNSGPSISSVDNNAPWIMTVAASYTDRRFPTTVKLG 360

Query: 368 NGKVFEGSSLYFGKNINEIPLVYNNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGTNSR 427
           NG+ FEG+SLY GK   ++PLVY  TAG GE +  C  GSL   +VKGK+VVC+RG N R
Sbjct: 361 NGQTFEGASLYTGKATAQLPLVYAGTAG-GEGAEYCIIGSLKKKLVKGKMVVCKRGMNGR 420

Query: 428 TAKGEQVKLAGGAGMILINTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQAKA 487
             KGEQVKLAGG GM+LINT+  GEEL AD+H LPAT++GASA  A+ +Y+ S+K +A A
Sbjct: 421 AEKGEQVKLAGGTGMLLINTETGGEELFADAHFLPATSLGASAGIAVKEYMNSTK-RATA 480

Query: 488 SITFKGTKYGSQAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDK 547
           SI FKGT YG+ AP +AAFSSRGP                  ILAAWPP+ SP+ L+SDK
Sbjct: 481 SIAFKGTVYGNPAPMLAAFSSRGPXXXXXXXXXXXXXXXXXXILAAWPPMTSPTLLKSDK 540

Query: 548 RRVLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDVSR 607
           R VLFN+ISGTSMSCPHVSGLAALLKS HK WSPAAIKSALMTTAYVTDN+   I+D   
Sbjct: 541 RSVLFNVISGTSMSCPHVSGLAALLKSVHKTWSPAAIKSALMTTAYVTDNRGSPIADAGS 600

Query: 608 ASGGPADPYAFGSGHVDPEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGKFTCS 667
           ++   A P+AFGSGHVDPE AS+PGL+YDI  +DY+NY CSL Y S+QIA VSR   TC 
Sbjct: 601 SNSASATPFAFGSGHVDPESASDPGLIYDITIEDYLNYFCSLNYTSSQIAQVSRRNVTCP 660

Query: 668 SKRKFLQPGDLNYPSFSLFMKKKAKNVTITFKRTVTNVGIPRSDYTAKINNPEGIRVIVK 727
              K LQPGDLNYPSF++  +  A+N  + +KRT+TNVG P S Y  K+  P G+ VI++
Sbjct: 661 D-NKALQPGDLNYPSFAVNFEGNARNNRVKYKRTLTNVGTPWSTYAVKVEEPNGVSVILE 720

Query: 728 PEKLSFVRLGGKLSYKVSFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVTWQ 773
           P+ LSF +LG KLSY V+FV+   +      SFGSLVW SGKY+VRSPIAVTWQ
Sbjct: 721 PKSLSFEKLGQKLSYNVTFVSSRGKGREGSSSFGSLVWLSGKYSVRSPIAVTWQ 769

BLAST of Bhi07G000258 vs. NCBI nr
Match: XP_008454764.1 (PREDICTED: subtilisin-like protease SBT1.1 [Cucumis melo] >XP_008454765.1 PREDICTED: subtilisin-like protease SBT1.1 [Cucumis melo])

HSP 1 Score: 1312.7 bits (3396), Expect = 0.0e+00
Identity = 658/772 (85.23%), Postives = 708/772 (91.71%), Query Frame = 0

Query: 1   MKIRQMLMFLSITIAILATSSAAVDQQSYIIHMDTSKMATANNPEQWYTVMIDSVNELAS 60
           M  +++L+FL IT  +L TS  A+DQQSYI+HMDT+KMAT  NPEQWYT +I SVN+L+S
Sbjct: 1   MGFKEVLLFLYIT--MLTTSIVAMDQQSYIVHMDTTKMAT-TNPEQWYTAIIHSVNKLSS 60

Query: 61  LDNDNNEEEASTAAEILYVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTTH 120
           LD +NNEE+A   AEILYVYKT ISGF AKLS+K+LH LSK+PGF+AA+PN+LLQLHTTH
Sbjct: 61  LDANNNEEQALNIAEILYVYKTVISGFCAKLSSKNLHFLSKVPGFIAASPNELLQLHTTH 120

Query: 121 SPQFLGLKRGRGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGPK 180
           SP+FLGL+RG GLWNSSNLASDIIIG+LDTGIWPEHISFQDK L PVP+KWKGICQ GP 
Sbjct: 121 SPKFLGLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKALPPVPSKWKGICQTGPS 180

Query: 181 FSPSNCNKKLIGARAFIQGYEAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRASF 240
           FS SNCNKKLIGAR FIQ YEA VGRLN TGTFRS RDSDGHGTHTASTAAGNFVNRASF
Sbjct: 181 FSLSNCNKKLIGARTFIQAYEAAVGRLNATGTFRSARDSDGHGTHTASTAAGNFVNRASF 240

Query: 241 YNQALGAATGMRFTSRIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLGGGSGIFY 300
           YNQ +G ATGMRFTSRIAAYKVCWPEGCASADILAAMDHA+ADGVDVLSISLGGGS I Y
Sbjct: 241 YNQGMGVATGMRFTSRIAAYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSIIY 300

Query: 301 SDEIAIAAFGAIQQGVFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGNG 360
           SD+IAIAAFGAIQ+GVFVSCSAGNSGP+ISTVGNVAPW+MTVAASYTDRTFPTTV+LGNG
Sbjct: 301 SDQIAIAAFGAIQKGVFVSCSAGNSGPFISTVGNVAPWVMTVAASYTDRTFPTTVRLGNG 360

Query: 361 KVFEGSSLYFGKNINEIPLVYNNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGTNSRTA 420
            VFEGSSLYFGKN+ E PLVYNNTAGDG E+N CT GSL P+MVKGKI VCERGTNSRT 
Sbjct: 361 MVFEGSSLYFGKNLKETPLVYNNTAGDGRETNFCTPGSLDPTMVKGKIAVCERGTNSRTE 420

Query: 421 KGEQVKLAGGAGMILINTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQAKASI 480
           KGEQVKLAGGAGMILINT LEGE+L+ADSHVLPAT+VG SA K+I++YIASSK QAKASI
Sbjct: 421 KGEQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGVSAGKSILNYIASSKRQAKASI 480

Query: 481 TFKGTKYGSQAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRR 540
            FKGTKYGS+APRVAAFSSRGPSF+KPYVIKPDITAPGVNILAAWPPIVSPSELESDKRR
Sbjct: 481 MFKGTKYGSRAPRVAAFSSRGPSFYKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRR 540

Query: 541 VLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDVSRAS 600
           VLFNIISGTSMSCPHVSGLAALLKS HKDWSPAAIKSALMTTAYVTDNKNHLISDV RAS
Sbjct: 541 VLFNIISGTSMSCPHVSGLAALLKSVHKDWSPAAIKSALMTTAYVTDNKNHLISDVGRAS 600

Query: 601 GGPADPYAFGSGHVDPEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGKFTCSSK 660
           GGPADP+AFGSGHVDPEKAS+PGLVYDIAPQDYI YLCSLKYNS QIALVSRGKFTCSSK
Sbjct: 601 GGPADPFAFGSGHVDPEKASDPGLVYDIAPQDYITYLCSLKYNSTQIALVSRGKFTCSSK 660

Query: 661 RKFLQPGDLNYPSFSLFMKKKAKNVTITFKRTVTNVGIPRSDYTAKINNPEGIRVIVKPE 720
           R F QPGDLNYPSFS+FM KK KNV  TFKRTVTNVGIPRSDYT +I NP+GIR+IVKPE
Sbjct: 661 RTFFQPGDLNYPSFSVFM-KKGKNVNSTFKRTVTNVGIPRSDYTVRIYNPKGIRIIVKPE 720

Query: 721 KLSFVRLGGKLSYKVSFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVTWQ 773
           KLSFV+LG KLSYKVSFVALGKR++LDDFSFGSLVW SG Y VRSPIAVTWQ
Sbjct: 721 KLSFVKLGEKLSYKVSFVALGKRKSLDDFSFGSLVWHSGTYVVRSPIAVTWQ 768

BLAST of Bhi07G000258 vs. NCBI nr
Match: XP_004140477.2 (PREDICTED: subtilisin-like protease SBT1.1 [Cucumis sativus] >XP_011659836.1 PREDICTED: subtilisin-like protease SBT1.1 [Cucumis sativus] >XP_011659837.1 PREDICTED: subtilisin-like protease SBT1.1 [Cucumis sativus])

HSP 1 Score: 1303.1 bits (3371), Expect = 0.0e+00
Identity = 652/773 (84.35%), Postives = 712/773 (92.11%), Query Frame = 0

Query: 1   MKIRQMLMFLSITIAILATSSAAVDQQSYIIHMDTSKMATANNPEQWYTVMIDSVNELAS 60
           M  +++L+ L IT  +L TSS A+DQQ+YI+HMDT+KM T  NPEQWYT +IDSVN+L+S
Sbjct: 1   MGFKEVLLLLYIT--MLTTSSVAMDQQTYIVHMDTTKMDTP-NPEQWYTAIIDSVNQLSS 60

Query: 61  LDNDNN-EEEASTAAEILYVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTT 120
           L  DNN +EEA  AAEILYVYKT ISGF+AKLS+++LHSLSK+PGF+AATPN+LLQLHTT
Sbjct: 61  LYGDNNDDEEALNAAEILYVYKTVISGFSAKLSSRNLHSLSKVPGFVAATPNELLQLHTT 120

Query: 121 HSPQFLGLKRGRGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGP 180
           HSPQFLGL+RG GLWNSSNLASDIIIG+LDTGIWPEHISFQDKGL PVP+KWKGICQ GP
Sbjct: 121 HSPQFLGLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPSKWKGICQTGP 180

Query: 181 KFSPSNCNKKLIGARAFIQGYEAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRAS 240
            FS SNCNKKLIGAR FIQ YEA VGRLN TG FRS RDS+GHGTHTASTAAGNF+NRAS
Sbjct: 181 NFSHSNCNKKLIGARTFIQAYEAAVGRLNGTGIFRSARDSNGHGTHTASTAAGNFINRAS 240

Query: 241 FYNQALGAATGMRFTSRIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLGGGSGIF 300
           FYNQ +G ATGMRFTSRIA+YKVCWPEGCASADILAAMDHA+ADGVDVLSISLGGGS I 
Sbjct: 241 FYNQGMGVATGMRFTSRIASYKVCWPEGCASADILAAMDHAVADGVDVLSISLGGGSSII 300

Query: 301 YSDEIAIAAFGAIQQGVFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGN 360
           YSD+IAIAAFGAIQ+GVFVSCSAGNSGP+ISTV NVAPW+MTVAASYTDRTFPTTV+LGN
Sbjct: 301 YSDQIAIAAFGAIQKGVFVSCSAGNSGPFISTVSNVAPWVMTVAASYTDRTFPTTVRLGN 360

Query: 361 GKVFEGSSLYFGKNINEIPLVYNNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGTNSRT 420
           GKVFEGSS YFGKN+ E+PLVYNNTAGDG+E+N CTAGSL P+MV+GKIVVCERGTNSRT
Sbjct: 361 GKVFEGSSSYFGKNLKEVPLVYNNTAGDGQETNFCTAGSLDPTMVRGKIVVCERGTNSRT 420

Query: 421 AKGEQVKLAGGAGMILINTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQAKAS 480
            KGEQVKLAGGAGMILINT LEGE+L+ADSHVLPAT+VGASA+K+I++YIASSK QAKAS
Sbjct: 421 KKGEQVKLAGGAGMILINTILEGEDLLADSHVLPATSVGASAAKSILNYIASSKRQAKAS 480

Query: 481 ITFKGTKYGSQAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKR 540
           I FKGTKYGS+APRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKR
Sbjct: 481 IIFKGTKYGSRAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKR 540

Query: 541 RVLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDVSRA 600
           RVLFNIISGTSMSCPHVSGLAAL+KS HKDWSPAAIKSALMTTAYVTDNK HLISDV RA
Sbjct: 541 RVLFNIISGTSMSCPHVSGLAALVKSVHKDWSPAAIKSALMTTAYVTDNKKHLISDVGRA 600

Query: 601 SGGPADPYAFGSGHVDPEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGKFTCSS 660
           SGGPAD +AFGSGHVDPEKAS+PGL+YDIAPQDYI YLCSLKY S QI+LVSRGKFTCSS
Sbjct: 601 SGGPADSFAFGSGHVDPEKASHPGLIYDIAPQDYITYLCSLKYTSTQISLVSRGKFTCSS 660

Query: 661 KRKFLQPGDLNYPSFSLFMKKKAKNVTITFKRTVTNVGIPRSDYTAKINNPEGIRVIVKP 720
           K  F QPGDLNYPSFS+FM KK KNV  TFKRTVTNVGIPRSDYT +INNP+GIR+IVKP
Sbjct: 661 KNTFSQPGDLNYPSFSVFM-KKGKNVNSTFKRTVTNVGIPRSDYTVRINNPKGIRIIVKP 720

Query: 721 EKLSFVRLGGKLSYKVSFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVTWQ 773
           EKL+FV+LG KLSYKVSF ALGKRE+LD+FSFGSLVW SG YAVRSPIAVTWQ
Sbjct: 721 EKLNFVKLGEKLSYKVSFYALGKRESLDEFSFGSLVWHSGTYAVRSPIAVTWQ 769

BLAST of Bhi07G000258 vs. NCBI nr
Match: XP_022989707.1 (subtilisin-like protease SBT1.1 [Cucurbita maxima] >XP_022989715.1 subtilisin-like protease SBT1.1 [Cucurbita maxima])

HSP 1 Score: 1233.8 bits (3191), Expect = 0.0e+00
Identity = 621/766 (81.07%), Postives = 684/766 (89.30%), Query Frame = 0

Query: 7   LMFLSITIAILATSSAAVDQQSYIIHMDTSKMATANNPEQWYTVMIDSVNELASLDNDNN 66
           L+ LSI   +LATS+AAVDQQ+YIIHMD +KMAT  NPEQWYT +I S+N+L+S+++D N
Sbjct: 8   LLLLSI---MLATSAAAVDQQTYIIHMDATKMAT-TNPEQWYTSIIHSINQLSSINDDQN 67

Query: 67  EEEASTAAEILYVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTTHSPQFLG 126
             EAS AAEILY+YKTAISGF+AKLST+ LHSLSK+PGFLAATP+KLLQLHTTH+PQFLG
Sbjct: 68  --EASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLAATPDKLLQLHTTHTPQFLG 127

Query: 127 LKRGRGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGPKFSPSNC 186
           L+RG GLWN+SNLASDIIIG++DTGIWPEHISFQDKGL PVP KWKG CQAGPKFS SNC
Sbjct: 128 LQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPPVPKKWKGTCQAGPKFSRSNC 187

Query: 187 NKKLIGARAFIQGYEAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRASFYNQALG 246
           NKKL+GARA+I+GYE ++GRLN TGTFRS RDSDGHGTHTASTAAGN V +AS YNQ +G
Sbjct: 188 NKKLVGARAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGMG 247

Query: 247 AATGMRFTSRIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLGGGSGIFYSDEIAI 306
           AATGMRFTSRIAAYKVCWPEGCAS DILAA+D A+ DGVDVLS+SLGGG G FY DEIAI
Sbjct: 248 AATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEIAI 307

Query: 307 AAFGAIQQGVFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGNGKVFEGS 366
           AAFGA++ GVFVSCSAGNSGP++STVGNVAPWIMTVAASYTDRTFP +VKLGNG++FEGS
Sbjct: 308 AAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFPGSVKLGNGQIFEGS 367

Query: 367 SLYFGKNINEIPLVYNNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQVK 426
           SL+ G +I E+PLVYN TAG GEE++VCTAGSLVPSMVKGKIVVCERGTNSR  KGEQVK
Sbjct: 368 SLHSGNSIGELPLVYNKTAG-GEEASVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQVK 427

Query: 427 LAGGAGMILINTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQAKASITFKGTK 486
           LAGG GMILINTQLEGEEL  DSHVLPA  +GASA KAII+YIASSKH  KASI F+GT+
Sbjct: 428 LAGGVGMILINTQLEGEELFGDSHVLPAINLGASAGKAIINYIASSKHPPKASILFEGTR 487

Query: 487 YGSQAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNII 546
           YGS+APR+AAFSSRGPSFF+PYVIKPDITAPGVNILAAWPP+VSPSEL+SDKRRVLFNII
Sbjct: 488 YGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFNII 547

Query: 547 SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDVSRASGGPADP 606
           SGTSMSCPHVSG+AALLKSAHK+WSPAAIKSALMTTAYV DNK  LISDV R SGGPADP
Sbjct: 548 SGTSMSCPHVSGIAALLKSAHKNWSPAAIKSALMTTAYVNDNKRSLISDVGRPSGGPADP 607

Query: 607 YAFGSGHVDPEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGKFTCSSKRKFLQP 666
           YAFGSGHVDPEKA +PGLVYDI PQDY+NYLCSL Y S Q+ LVSRG F+C SKR  LQP
Sbjct: 608 YAFGSGHVDPEKAVDPGLVYDIVPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSKRTVLQP 667

Query: 667 GDLNYPSFSLFMKKKAKNVTITFKRTVTNVGIPRSDYTAKINNPEGIRVIVKPEKLSFVR 726
           GDLNYPSFS+ MK KAKNV   FKRTVTNVG P SDYT KINNP GIRV VKPEKLSF R
Sbjct: 668 GDLNYPSFSVSMKNKAKNV--RFKRTVTNVGTPSSDYTVKINNPSGIRVSVKPEKLSFRR 727

Query: 727 LGGKLSYKVSFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVTWQ 773
            G KLSY+VSFVALGKRE L DFSFGSLVW SGKY+VRSPIAV W+
Sbjct: 728 SGQKLSYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Bhi07G000258 vs. NCBI nr
Match: XP_008454762.1 (PREDICTED: subtilisin-like protease SBT1.1 [Cucumis melo] >XP_016901624.1 PREDICTED: subtilisin-like protease SBT1.1 [Cucumis melo])

HSP 1 Score: 1218.4 bits (3151), Expect = 0.0e+00
Identity = 618/757 (81.64%), Postives = 669/757 (88.38%), Query Frame = 0

Query: 16  ILATSSAAVDQQSYIIHMDTSKMATANNPEQWYTVMIDSVNELASLDNDNNEEEASTAAE 75
           +LA SSA VDQQ+YIIHMDT+KM T  NPEQWYT +IDSVNEL+SLD++   EEAS AAE
Sbjct: 12  MLAISSAVVDQQTYIIHMDTTKMVTP-NPEQWYTDIIDSVNELSSLDDN---EEASNAAE 71

Query: 76  ILYVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTTHSPQFLGLKRGRGLWN 135
           ILYVYKTA+SGFAAKL++K LHSLSKIPGFLAATPN+LLQLHTTHSPQFLGL+R  GLWN
Sbjct: 72  ILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDHGLWN 131

Query: 136 SSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGPKFSPSNCNKKLIGARA 195
            SNLASDIIIGLLDTGIWPEHISFQDKGLS VP KWKGICQ GP+FS SNCNKKLIGA A
Sbjct: 132 FSNLASDIIIGLLDTGIWPEHISFQDKGLSSVPLKWKGICQTGPRFSSSNCNKKLIGASA 191

Query: 196 FIQGYEAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRASFYNQALGAATGMRFTS 255
           +I+GYEA+VGRLNETGTFRSPRDSDGHGTHTASTAAG+ V+ ASFYNQ +G A+GMRFTS
Sbjct: 192 YIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGSIVDNASFYNQGMGVASGMRFTS 251

Query: 256 RIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLGGGSGIFYSDEIAIAAFGAIQQG 315
           RI AYKVCWP GCA+ADILAAMD A+ADGVDVLS+SLGGGS  FY D IAIAAFGAIQ+G
Sbjct: 252 RIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAIAAFGAIQKG 311

Query: 316 VFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGNGKVFEGSSLYFGKNIN 375
           VFVSCSAGNSGP  STVGN APWIMTVAASYTDRTFPTTVKLGNG+VFEGSSLY+GK+IN
Sbjct: 312 VFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSSLYYGKSIN 371

Query: 376 EIPLVYNNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQVKLAGGAGMIL 435
           E+PLVYNNTAGDG+E+NVC AGSL PSMVKGKIV+CERGT SRT KGEQVKLAGG GMIL
Sbjct: 372 ELPLVYNNTAGDGQETNVCIAGSLDPSMVKGKIVICERGTISRTEKGEQVKLAGGTGMIL 431

Query: 436 INTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQAKASITFKGTKYGSQAPRVA 495
           INTQ EGEEL AD HVLPAT +GASA KAI+DYIASSK QAKASI F+GTKYGSQAPRVA
Sbjct: 432 INTQFEGEELFADPHVLPATTLGASAGKAILDYIASSKTQAKASIVFEGTKYGSQAPRVA 491

Query: 496 AFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPH 555
           AFSSRGPS                NILAAWPPIVSPSEL SD RRV+FNIISGTSMSCPH
Sbjct: 492 AFSSRGPSLXXXXXXXXXXXXXXXNILAAWPPIVSPSELASDTRRVMFNIISGTSMSCPH 551

Query: 556 VSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDVSRASGGPADPYAFGSGHVD 615
           VSGLAALLKSAH DWSPAAIKSALMTTAYVTD+K  LISDV +A+G PA P+ FGSGHVD
Sbjct: 552 VSGLAALLKSAHNDWSPAAIKSALMTTAYVTDSKMSLISDVGQANGEPATPFTFGSGHVD 611

Query: 616 PEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGKFTCSSKRKFLQPGDLNYPSFS 675
           PEKAS+PGL+YDI PQDYINYLCSLKYNS+QIALVSRG  TCSSKR  ++PGDLNYPSFS
Sbjct: 612 PEKASDPGLIYDITPQDYINYLCSLKYNSSQIALVSRGNLTCSSKRTVVKPGDLNYPSFS 671

Query: 676 LFMKKKAKNVTITFKRTVTNVGIPRSDYTAKINNPEGIRVIVKPEKLSFVRLGGKLSYKV 735
           +FMKKKAK V+IT KRTVTNVGI RSDYT KINNP+G+ VIVKPEKLSF  LG +LSYKV
Sbjct: 672 VFMKKKAKKVSITLKRTVTNVGITRSDYTVKINNPKGVTVIVKPEKLSFGSLGEQLSYKV 731

Query: 736 SFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVTWQ 773
           SFV+LG +E LD FSFGSLVW SGKYAVRSPI VTWQ
Sbjct: 732 SFVSLGGKEALDKFSFGSLVWISGKYAVRSPIVVTWQ 764

BLAST of Bhi07G000258 vs. NCBI nr
Match: XP_023544752.1 (subtilisin-like protease SBT1.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1214.9 bits (3142), Expect = 0.0e+00
Identity = 614/772 (79.53%), Postives = 685/772 (88.73%), Query Frame = 0

Query: 1   MKIRQMLMFLSITIAILATSSAAVDQQSYIIHMDTSKMATANNPEQWYTVMIDSVNELAS 60
           M I ++ + LSI   +LATS+AAVDQQSYIIHMDT+KMAT  NP+QWYT +ID+VN+L+S
Sbjct: 1   MGITELWLLLSI---MLATSAAAVDQQSYIIHMDTTKMAT-TNPQQWYTSIIDTVNQLSS 60

Query: 61  LDNDNNEEEASTAAEILYVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTTH 120
           +++D N  EAS AAEILY+YKTAISGF+AKLST+ L SLSK+PGFL+ATP+KLLQLHTTH
Sbjct: 61  INDDQN--EASNAAEILYIYKTAISGFSAKLSTRKLLSLSKLPGFLSATPDKLLQLHTTH 120

Query: 121 SPQFLGLKRGRGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGPK 180
           +P+FLGL+RG GLWN+S LASDIIIG++DTGIWPEHISFQDKGLSPVP KWKG CQAGPK
Sbjct: 121 TPKFLGLQRGHGLWNASYLASDIIIGVIDTGIWPEHISFQDKGLSPVPKKWKGTCQAGPK 180

Query: 181 FSPSNCNKKLIGARAFIQGYEAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRASF 240
           FS SNCN KLIGARA+I+GYE ++GRLN TGTFRS RDSDGHGTHTASTAAGN V +AS 
Sbjct: 181 FSRSNCNNKLIGARAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASL 240

Query: 241 YNQALGAATGMRFTSRIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLGGGSGIFY 300
           YNQ +GAATGMRFTSRIAAYKVCWPEGCAS DILAA+D A+ DGVDVLS+SLGGG G FY
Sbjct: 241 YNQGMGAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFY 300

Query: 301 SDEIAIAAFGAIQQGVFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGNG 360
            DEIAIAAFGA++ GVFVSCSAGNSGP++STVGNVAPWIMTVAASYTDRTF  +VKLGNG
Sbjct: 301 QDEIAIAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFAGSVKLGNG 360

Query: 361 KVFEGSSLYFGKNINEIPLVYNNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGTNSRTA 420
           ++FEGSSL+ G +I ++PLVYN TAG GEE+NVCTAGSLVPSMVKGKIVVCERGTNSR  
Sbjct: 361 QIFEGSSLHSGNSIGQLPLVYNKTAG-GEEANVCTAGSLVPSMVKGKIVVCERGTNSRFE 420

Query: 421 KGEQVKLAGGAGMILINTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQAKASI 480
           KGEQVKLAGG GMILINTQLEGEEL ADSHVLPA  +GASA KAI +YIASSK   KASI
Sbjct: 421 KGEQVKLAGGVGMILINTQLEGEELFADSHVLPAVNLGASAGKAITNYIASSKQPPKASI 480

Query: 481 TFKGTKYGSQAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRR 540
            F+GT+YGS+APR+AAFSSRGPSFF+PYVIKPDITAPGVNILAAWPP+VSPSEL+SD+RR
Sbjct: 481 LFEGTRYGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDERR 540

Query: 541 VLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDVSRAS 600
           VLFNIISGTSMSCPHVSG+AALLKSAHK+WSPAAIKSALMTTAYVTDNK  +ISDV R S
Sbjct: 541 VLFNIISGTSMSCPHVSGIAALLKSAHKNWSPAAIKSALMTTAYVTDNKRSVISDVGRPS 600

Query: 601 GGPADPYAFGSGHVDPEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGKFTCSSK 660
           GGPADPYAFGSGHVDPEKA +PGLVYDIAPQDY+NYLCSL Y S Q+ LVSRG F+C SK
Sbjct: 601 GGPADPYAFGSGHVDPEKAVDPGLVYDIAPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSK 660

Query: 661 RKFLQPGDLNYPSFSLFMKKKAKNVTITFKRTVTNVGIPRSDYTAKINNPEGIRVIVKPE 720
              LQPGDLNYPSFS+ MK +AKNVT+  KRTVTNVG P SDYT KINNP GI V VKP+
Sbjct: 661 GTVLQPGDLNYPSFSVSMKNRAKNVTL--KRTVTNVGTPTSDYTVKINNPSGIAVSVKPK 720

Query: 721 KLSFVRLGGKLSYKVSFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVTWQ 773
           KLSF R G KLSY+VSFVA GKRE L DFSFGSLVW SGKY+VRSPIAV W+
Sbjct: 721 KLSFRRSGQKLSYQVSFVASGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 763