Bhi06G001266 (gene) Wax gourd

NameBhi06G001266
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionLipoxygenase
Locationchr6 : 43034603 .. 43035010 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAAATTTCATTCCTCCACCCAACAGCTTGTTCTTCAGGCTATTAAAACAAAGAGAAGGAAAGAGTGGGAAAAAAAGATTGTTAGAAAAAAAAAAAAAAAAAAAGTGAAAATGTTTTCTATTGGAAAGAATATCATAGAGGGAGCCTTGAATACTACTGGAGATCTTGCTGGCTCTGTTATCAATGCTGGAAGTAACATTGCAGATCAAATTTCTAATCTTGGAGGAAAGAAAATCAAAGGAAAAGTCATTCTTATGAGAAGCAATGTTTTGGATTTCACTGAGTTTCATTCCTCTCTTCTTGATAATTTCACTGAACTCTTGGGCGGTGGTGTTTCTCTTCAACTCATTAGTGCCACTCAAGCTTGTAACATCTCTTTCCCTTCAACTCTCTATATATTTGTTTGA

mRNA sequence

GAAAAATTTCATTCCTCCACCCAACAGCTTGTTCTTCAGGCTATTAAAACAAAGAGAAGGAAAGAGTGGGAAAAAAAGATTGTTAGAAAAAAAAAAAAAAAAAAAGTGAAAATGTTTTCTATTGGAAAGAATATCATAGAGGGAGCCTTGAATACTACTGGAGATCTTGCTGGCTCTGTTATCAATGCTGGAAGTAACATTGCAGATCAAATTTCTAATCTTGGAGGAAAGAAAATCAAAGGAAAAGTCATTCTTATGAGAAGCAATGTTTTGGATTTCACTGAGTTTCATTCCTCTCTTCTTGATAATTTCACTGAACTCTTGGGCGGTGGTGTTTCTCTTCAACTCATTAGTGCCACTCAAGCTTGTAACATCTCTTTCCCTTCAACTCTCTATATATTTGTTTGA

Coding sequence (CDS)

ATGTTTTCTATTGGAAAGAATATCATAGAGGGAGCCTTGAATACTACTGGAGATCTTGCTGGCTCTGTTATCAATGCTGGAAGTAACATTGCAGATCAAATTTCTAATCTTGGAGGAAAGAAAATCAAAGGAAAAGTCATTCTTATGAGAAGCAATGTTTTGGATTTCACTGAGTTTCATTCCTCTCTTCTTGATAATTTCACTGAACTCTTGGGCGGTGGTGTTTCTCTTCAACTCATTAGTGCCACTCAAGCTTGTAACATCTCTTTCCCTTCAACTCTCTATATATTTGTTTGA

Protein sequence

MFSIGKNIIEGALNTTGDLAGSVINAGSNIADQISNLGGKKIKGKVILMRSNVLDFTEFHSSLLDNFTELLGGGVSLQLISATQACNISFPSTLYIFV
BLAST of Bhi06G001266 vs. Swiss-Prot
Match: sp|Q43191|LOX15_SOLTU (Probable linoleate 9S-lipoxygenase 5 OS=Solanum tuberosum OX=4113 GN=LOX1.5 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 1.4e-07
Identity = 27/47 (57.45%), Postives = 34/47 (72.34%), Query Frame = 0

Query: 39 GKKIKGKVILMRSNVLDFTEFHSSLLDNFTELLGGGVSLQLISATQA 86
          GKK+KG ++LM+ NVLDF + ++SLLD   E LG  VSLQLIS   A
Sbjct: 18 GKKVKGTIVLMKKNVLDFNDVNASLLDGVLEFLGKRVSLQLISVVHA 64

BLAST of Bhi06G001266 vs. Swiss-Prot
Match: sp|Q06327|LOX1_ARATH (Linoleate 9S-lipoxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=LOX1 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 4.4e-06
Identity = 22/43 (51.16%), Postives = 33/43 (76.74%), Query Frame = 0

Query: 40 KKIKGKVILMRSNVLDFTEFHSSLLDNFTELLGGGVSLQLISA 83
          KK+KG V+LM+ NVLDF +F++S LD   E LG  ++L+L+S+
Sbjct: 19 KKVKGTVVLMKKNVLDFNDFNASFLDRLHEFLGNKITLRLVSS 61

BLAST of Bhi06G001266 vs. Swiss-Prot
Match: sp|Q43190|LOX14_SOLTU (Probable linoleate 9S-lipoxygenase 4 OS=Solanum tuberosum OX=4113 GN=LOX1.4 PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 9.8e-06
Identity = 24/46 (52.17%), Postives = 31/46 (67.39%), Query Frame = 0

Query: 40 KKIKGKVILMRSNVLDFTEFHSSLLDNFTELLGGGVSLQLISATQA 86
          KK+KG V++M+ N LDFT+   SL D   E LG  VS QLIS+ Q+
Sbjct: 17 KKVKGTVVMMKKNALDFTDLAGSLTDKIFEALGQKVSFQLISSVQS 62

BLAST of Bhi06G001266 vs. Swiss-Prot
Match: sp|Q41238|LOX16_SOLTU (Linoleate 9S-lipoxygenase 6 (Fragment) OS=Solanum tuberosum OX=4113 GN=LOX1.6 PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 9.8e-06
Identity = 24/46 (52.17%), Postives = 31/46 (67.39%), Query Frame = 0

Query: 40 KKIKGKVILMRSNVLDFTEFHSSLLDNFTELLGGGVSLQLISATQA 86
          KK+KG V++M+ N LDFT+   SL D   E LG  VS QLIS+ Q+
Sbjct: 14 KKVKGTVVMMKKNALDFTDLAGSLTDKIFEALGQKVSFQLISSVQS 59

BLAST of Bhi06G001266 vs. Swiss-Prot
Match: sp|P38415|LOXA_SOLLC (Linoleate 9S-lipoxygenase A OS=Solanum lycopersicum OX=4081 GN=LOX1.1 PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 9.8e-06
Identity = 24/46 (52.17%), Postives = 31/46 (67.39%), Query Frame = 0

Query: 40 KKIKGKVILMRSNVLDFTEFHSSLLDNFTELLGGGVSLQLISATQA 86
          KK+KG V++M+ N LDFT+   SL D   E LG  VS QLIS+ Q+
Sbjct: 17 KKVKGTVVMMKKNALDFTDLAGSLTDKIFEALGQKVSFQLISSVQS 62

BLAST of Bhi06G001266 vs. TAIR10
Match: AT1G55020.1 (lipoxygenase 1)

HSP 1 Score: 52.0 bits (123), Expect = 2.4e-07
Identity = 22/43 (51.16%), Postives = 33/43 (76.74%), Query Frame = 0

Query: 40 KKIKGKVILMRSNVLDFTEFHSSLLDNFTELLGGGVSLQLISA 83
          KK+KG V+LM+ NVLDF +F++S LD   E LG  ++L+L+S+
Sbjct: 19 KKVKGTVVLMKKNVLDFNDFNASFLDRLHEFLGNKITLRLVSS 61

BLAST of Bhi06G001266 vs. TAIR10
Match: AT3G22400.1 (PLAT/LH2 domain-containing lipoxygenase family protein)

HSP 1 Score: 48.9 bits (115), Expect = 2.1e-06
Identity = 24/44 (54.55%), Postives = 33/44 (75.00%), Query Frame = 0

Query: 41 KIKGKVILMRSNVLDFTEFHSSLLDNFTELLGGGVSLQLISATQ 85
          KI+G+V++M+ N+LDF +  +SLLD   ELLG  VSL LIS+ Q
Sbjct: 34 KIEGEVVVMKKNLLDFKDVMASLLDRVNELLGRRVSLHLISSHQ 77

BLAST of Bhi06G001266 vs. TrEMBL
Match: tr|A0A0A0LGD7|A0A0A0LGD7_CUCSA (Lipoxygenase OS=Cucumis sativus OX=3659 GN=Csa_2G027460 PE=4 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 4.5e-40
Identity = 87/91 (95.60%), Postives = 89/91 (97.80%), Query Frame = 0

Query: 1  MFSIGKNIIEGALNTTGDLAGSVINAGSNIADQISNLGGKKIKGKVILMRSNVLDFTEFH 60
          MFSIGKNIIEGALNTTGDLAGSVINAGSNIADQISN+GG+KIKGKVILMRSNVLDFTEFH
Sbjct: 1  MFSIGKNIIEGALNTTGDLAGSVINAGSNIADQISNIGGQKIKGKVILMRSNVLDFTEFH 60

Query: 61 SSLLDNFTELLGGGVSLQLISATQACNISFP 92
          SSLLDNFTELLGGGVSLQLISATQ CN SFP
Sbjct: 61 SSLLDNFTELLGGGVSLQLISATQTCNFSFP 91

BLAST of Bhi06G001266 vs. TrEMBL
Match: tr|A0A1S3BZJ7|A0A1S3BZJ7_CUCME (Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC103494902 PE=3 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 9.7e-35
Identity = 79/89 (88.76%), Postives = 82/89 (92.13%), Query Frame = 0

Query: 1  MFSIGKNIIEGALNTTGDLAGSVINAGSNIADQISNLGGKKIKGKVILMRSNVLDFTEFH 60
          MF IGKNIIEGALNTTGDLAGSVINAG NI D++SNLGGKKIKGKVILMRSNVLDFTEFH
Sbjct: 1  MFGIGKNIIEGALNTTGDLAGSVINAGGNILDRVSNLGGKKIKGKVILMRSNVLDFTEFH 60

Query: 61 SSLLDNFTELLGGGVSLQLISATQACNIS 90
          SSLLDNFTELLGGGVS+QLISAT   N S
Sbjct: 61 SSLLDNFTELLGGGVSIQLISATHTSNDS 89

BLAST of Bhi06G001266 vs. TrEMBL
Match: tr|A0A1S3BZG7|A0A1S3BZG7_CUCME (Lipoxygenase OS=Cucumis melo OX=3656 GN=LOX2 PE=3 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.1e-33
Identity = 77/83 (92.77%), Postives = 78/83 (93.98%), Query Frame = 0

Query: 1  MFSIGKNIIEGALNTTGDLAGSVINAGSNIADQISNLGGKKIKGKVILMRSNVLDFTEFH 60
          MF IGKNIIEGA NTTGDLAGSVINAG N ADQISN GGKKIKGKVILMRSNVLDFTEFH
Sbjct: 1  MFGIGKNIIEGAFNTTGDLAGSVINAGGNFADQISNFGGKKIKGKVILMRSNVLDFTEFH 60

Query: 61 SSLLDNFTELLGGGVSLQLISAT 84
          SSLLDNFTELLGGGVS+QLISAT
Sbjct: 61 SSLLDNFTELLGGGVSIQLISAT 83

BLAST of Bhi06G001266 vs. TrEMBL
Match: tr|C8CBS6|C8CBS6_CUCME (Lipoxygenase OS=Cucumis melo var. inodorus OX=357961 GN=LOX2 PE=2 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.1e-33
Identity = 77/83 (92.77%), Postives = 78/83 (93.98%), Query Frame = 0

Query: 1  MFSIGKNIIEGALNTTGDLAGSVINAGSNIADQISNLGGKKIKGKVILMRSNVLDFTEFH 60
          MF IGKNIIEGA NTTGDLAGSVINAG N ADQISN GGKKIKGKVILMRSNVLDFTEFH
Sbjct: 1  MFGIGKNIIEGAFNTTGDLAGSVINAGGNFADQISNFGGKKIKGKVILMRSNVLDFTEFH 60

Query: 61 SSLLDNFTELLGGGVSLQLISAT 84
          SSLLDNFTELLGGGVS+QLISAT
Sbjct: 61 SSLLDNFTELLGGGVSIQLISAT 83

BLAST of Bhi06G001266 vs. TrEMBL
Match: tr|A0A0A0LFT8|A0A0A0LFT8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G023920 PE=4 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 4.1e-33
Identity = 73/91 (80.22%), Postives = 82/91 (90.11%), Query Frame = 0

Query: 1  MFSIGKNIIEGALNTTGDLAGSVINAGSNIADQISNLGGKKIKGKVILMRSNVLDFTEFH 60
          MF IGKNIIEGALNTTGDLAGSV+NAG NI D++S++G KKIKGKVILMRSNVLDFT+ H
Sbjct: 1  MFGIGKNIIEGALNTTGDLAGSVLNAGGNIVDKVSSIGDKKIKGKVILMRSNVLDFTQLH 60

Query: 61 SSLLDNFTELLGGGVSLQLISATQACNISFP 92
          SS+LD FTE+LG GV+ QLISATQACNIS P
Sbjct: 61 SSVLDTFTEILGSGVTFQLISATQACNISSP 91

BLAST of Bhi06G001266 vs. NCBI nr
Match: KGN60933.1 (Lipoxygenase [Cucumis sativus])

HSP 1 Score: 172.6 bits (436), Expect = 6.8e-40
Identity = 87/91 (95.60%), Postives = 89/91 (97.80%), Query Frame = 0

Query: 1  MFSIGKNIIEGALNTTGDLAGSVINAGSNIADQISNLGGKKIKGKVILMRSNVLDFTEFH 60
          MFSIGKNIIEGALNTTGDLAGSVINAGSNIADQISN+GG+KIKGKVILMRSNVLDFTEFH
Sbjct: 1  MFSIGKNIIEGALNTTGDLAGSVINAGSNIADQISNIGGQKIKGKVILMRSNVLDFTEFH 60

Query: 61 SSLLDNFTELLGGGVSLQLISATQACNISFP 92
          SSLLDNFTELLGGGVSLQLISATQ CN SFP
Sbjct: 61 SSLLDNFTELLGGGVSLQLISATQTCNFSFP 91

BLAST of Bhi06G001266 vs. NCBI nr
Match: XP_011650109.1 (PREDICTED: linoleate 9S-lipoxygenase 6-like [Cucumis sativus])

HSP 1 Score: 159.8 bits (403), Expect = 4.6e-36
Identity = 82/84 (97.62%), Postives = 84/84 (100.00%), Query Frame = 0

Query: 1  MFSIGKNIIEGALNTTGDLAGSVINAGSNIADQISNLGGKKIKGKVILMRSNVLDFTEFH 60
          MFSIGKNIIEGALNTTGDLAGSVINAGSNIADQISN+GG+KIKGKVILMRSNVLDFTEFH
Sbjct: 1  MFSIGKNIIEGALNTTGDLAGSVINAGSNIADQISNIGGQKIKGKVILMRSNVLDFTEFH 60

Query: 61 SSLLDNFTELLGGGVSLQLISATQ 85
          SSLLDNFTELLGGGVSLQLISATQ
Sbjct: 61 SSLLDNFTELLGGGVSLQLISATQ 84

BLAST of Bhi06G001266 vs. NCBI nr
Match: XP_008454513.1 (PREDICTED: linoleate 9S-lipoxygenase 6-like [Cucumis melo])

HSP 1 Score: 154.8 bits (390), Expect = 1.5e-34
Identity = 79/89 (88.76%), Postives = 82/89 (92.13%), Query Frame = 0

Query: 1  MFSIGKNIIEGALNTTGDLAGSVINAGSNIADQISNLGGKKIKGKVILMRSNVLDFTEFH 60
          MF IGKNIIEGALNTTGDLAGSVINAG NI D++SNLGGKKIKGKVILMRSNVLDFTEFH
Sbjct: 1  MFGIGKNIIEGALNTTGDLAGSVINAGGNILDRVSNLGGKKIKGKVILMRSNVLDFTEFH 60

Query: 61 SSLLDNFTELLGGGVSLQLISATQACNIS 90
          SSLLDNFTELLGGGVS+QLISAT   N S
Sbjct: 61 SSLLDNFTELLGGGVSIQLISATHTSNDS 89

BLAST of Bhi06G001266 vs. NCBI nr
Match: ACU81176.1 (13S-lipoxygenase [Cucumis melo var. inodorus])

HSP 1 Score: 151.4 bits (381), Expect = 1.6e-33
Identity = 77/83 (92.77%), Postives = 78/83 (93.98%), Query Frame = 0

Query: 1  MFSIGKNIIEGALNTTGDLAGSVINAGSNIADQISNLGGKKIKGKVILMRSNVLDFTEFH 60
          MF IGKNIIEGA NTTGDLAGSVINAG N ADQISN GGKKIKGKVILMRSNVLDFTEFH
Sbjct: 1  MFGIGKNIIEGAFNTTGDLAGSVINAGGNFADQISNFGGKKIKGKVILMRSNVLDFTEFH 60

Query: 61 SSLLDNFTELLGGGVSLQLISAT 84
          SSLLDNFTELLGGGVS+QLISAT
Sbjct: 61 SSLLDNFTELLGGGVSIQLISAT 83

BLAST of Bhi06G001266 vs. NCBI nr
Match: XP_008454470.1 (PREDICTED: linoleate 9S-lipoxygenase 6 [Cucumis melo])

HSP 1 Score: 151.4 bits (381), Expect = 1.6e-33
Identity = 77/83 (92.77%), Postives = 78/83 (93.98%), Query Frame = 0

Query: 1  MFSIGKNIIEGALNTTGDLAGSVINAGSNIADQISNLGGKKIKGKVILMRSNVLDFTEFH 60
          MF IGKNIIEGA NTTGDLAGSVINAG N ADQISN GGKKIKGKVILMRSNVLDFTEFH
Sbjct: 1  MFGIGKNIIEGAFNTTGDLAGSVINAGGNFADQISNFGGKKIKGKVILMRSNVLDFTEFH 60

Query: 61 SSLLDNFTELLGGGVSLQLISAT 84
          SSLLDNFTELLGGGVS+QLISAT
Sbjct: 61 SSLLDNFTELLGGGVSIQLISAT 83

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|Q43191|LOX15_SOLTU1.4e-0757.45Probable linoleate 9S-lipoxygenase 5 OS=Solanum tuberosum OX=4113 GN=LOX1.5 PE=2... [more]
sp|Q06327|LOX1_ARATH4.4e-0651.16Linoleate 9S-lipoxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=LOX1 PE=1 SV=1[more]
sp|Q43190|LOX14_SOLTU9.8e-0652.17Probable linoleate 9S-lipoxygenase 4 OS=Solanum tuberosum OX=4113 GN=LOX1.4 PE=2... [more]
sp|Q41238|LOX16_SOLTU9.8e-0652.17Linoleate 9S-lipoxygenase 6 (Fragment) OS=Solanum tuberosum OX=4113 GN=LOX1.6 PE... [more]
sp|P38415|LOXA_SOLLC9.8e-0652.17Linoleate 9S-lipoxygenase A OS=Solanum lycopersicum OX=4081 GN=LOX1.1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G55020.12.4e-0751.16lipoxygenase 1[more]
AT3G22400.12.1e-0654.55PLAT/LH2 domain-containing lipoxygenase family protein[more]
Match NameE-valueIdentityDescription
tr|A0A0A0LGD7|A0A0A0LGD7_CUCSA4.5e-4095.60Lipoxygenase OS=Cucumis sativus OX=3659 GN=Csa_2G027460 PE=4 SV=1[more]
tr|A0A1S3BZJ7|A0A1S3BZJ7_CUCME9.7e-3588.76Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC103494902 PE=3 SV=1[more]
tr|A0A1S3BZG7|A0A1S3BZG7_CUCME1.1e-3392.77Lipoxygenase OS=Cucumis melo OX=3656 GN=LOX2 PE=3 SV=1[more]
tr|C8CBS6|C8CBS6_CUCME1.1e-3392.77Lipoxygenase OS=Cucumis melo var. inodorus OX=357961 GN=LOX2 PE=2 SV=1[more]
tr|A0A0A0LFT8|A0A0A0LFT8_CUCSA4.1e-3380.22Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G023920 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
KGN60933.16.8e-4095.60Lipoxygenase [Cucumis sativus][more]
XP_011650109.14.6e-3697.62PREDICTED: linoleate 9S-lipoxygenase 6-like [Cucumis sativus][more]
XP_008454513.11.5e-3488.76PREDICTED: linoleate 9S-lipoxygenase 6-like [Cucumis melo][more]
ACU81176.11.6e-3392.7713S-lipoxygenase [Cucumis melo var. inodorus][more]
XP_008454470.11.6e-3392.77PREDICTED: linoleate 9S-lipoxygenase 6 [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO:0016702oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0046872metal ion binding
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: INTERPRO
TermDefinition
IPR036392PLAT/LH2_dom_sf
IPR000907LipOase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0031408 oxylipin biosynthetic process
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
molecular_function GO:0005515 protein binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi06M001266Bhi06M001266mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:2.60.60.20coord: 33..90
e-value: 8.3E-10
score: 40.6
NoneNo IPR availablePANTHERPTHR11771:SF53LINOLEATE 9S-LIPOXYGENASE 5, CHLOROPLASTICcoord: 29..85
IPR000907LipoxygenasePANTHERPTHR11771LIPOXYGENASEcoord: 29..85
IPR036392PLAT/LH2 domain superfamilySUPERFAMILYSSF49723Lipase/lipooxygenase domain (PLAT/LH2 domain)coord: 40..85

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None