Bhi06G000883 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi06G000883
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	cucumisin-like
Location	chr6 : 27363989 .. 27364464 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCATGTGAACCCACTAAAAGCAGTAAGACCTGGGTTGGTTTATGATGCAAATGAAAGCGATTATGTGAAATTCTTATGTGGTCAAGGCTACAGCACCAACATGGTTCGACGTATCACCGGAGACTACGGTGCTTGTACTTCTAGAAATATTGGTAGAGTATGGGATTTAAACTATCCTTCTTTTGGACTTTCAGTATCTCGTTCGCAGACCTTCAGTCAATACTTCACAAGAACTCTCACAAGTGTGGTATCTCAAGCATGGACATATAGAGCTATGATTTCTGCACCACAAGGCCTTGCTATCACAGTGAATCCTAATGTTCTATCATTTAATGGCATCGGAGATAAGAAATCTTTCACATTGACAATTCGAGGATCAGTAAGCCAGTCTGTAGTTTCTGCTTCTTTGGTGTGGACCAATGGTGTACATACTGTGAGAAGCCCCATAACAATCACGACTCTTAGTTTAGATTAG

mRNA sequence

GCCATGTGAACCCACTAAAAGCAGTAAGACCTGGGTTGGTTTATGATGCAAATGAAAGCGATTATGTGAAATTCTTATGTGGTCAAGGCTACAGCACCAACATGGTTCGACGTATCACCGGAGACTACGGTGCTTGTACTTCTAGAAATATTGGTAGAGTATGGGATTTAAACTATCCTTCTTTTGGACTTTCAGTATCTCGTTCGCAGACCTTCAGTCAATACTTCACAAGAACTCTCACAAGTGTGGTATCTCAAGCATGGACATATAGAGCTATGATTTCTGCACCACAAGGCCTTGCTATCACAGTGAATCCTAATGTTCTATCATTTAATGGCATCGGAGATAAGAAATCTTTCACATTGACAATTCGAGGATCAGTAAGCCAGTCTGTAGTTTCTGCTTCTTTGGTGTGGACCAATGGTGTACATACTGTGAGAAGCCCCATAACAATCACGACTCTTAGTTTAGATTAG

Coding sequence (CDS)

GCCATGTGAACCCACTAAAAGCAGTAAGACCTGGGTTGGTTTATGATGCAAATGAAAGCGATTATGTGAAATTCTTATGTGGTCAAGGCTACAGCACCAACATGGTTCGACGTATCACCGGAGACTACGGTGCTTGTACTTCTAGAAATATTGGTAGAGTATGGGATTTAAACTATCCTTCTTTTGGACTTTCAGTATCTCGTTCGCAGACCTTCAGTCAATACTTCACAAGAACTCTCACAAGTGTGGTATCTCAAGCATGGACATATAGAGCTATGATTTCTGCACCACAAGGCCTTGCTATCACAGTGAATCCTAATGTTCTATCATTTAATGGCATCGGAGATAAGAAATCTTTCACATTGACAATTCGAGGATCAGTAAGCCAGTCTGTAGTTTCTGCTTCTTTGGTGTGGACCAATGGTGTACATACTGTGAGAAGCCCCATAACAATCACGACTCTTAGTTTAGATTAG

Protein sequence

HVNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRVWDLNYPSFGLSVSRSQTFSQYFTRTLTSVVSQAWTYRAMISAPQGLAITVNPNVLSFNGIGDKKSFTLTIRGSVSQSVVSASLVWTNGVHTVRSPITITTLSLD

Homology

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BLAST of Bhi06G000883 vs. Swiss-Prot
Match: sp|Q39547|CUCM1_CUCME (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 5.9e-69
Identity = 129/154 (83.77%), Postives = 139/154 (90.26%), Query Frame = 0


Query: 1   HVNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRVWDLNYPS 60
           HVNPLKAVRPGLVYDANESDYVKFLCGQGY+T  VRRITGDY ACTS N GRVWDLNYPS
Sbjct: 577 HVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPS 636

Query: 61  FGLSVSRSQTFSQYFTRTLTSVVSQAWTYRAMISAPQGLAITVNPNVLSFNGIGDKKSFT 120
           FGLSVS SQTF+QYF RTLTSV  QA TYRAMISAPQGL I+VNPNVLSFNG+GD+KSFT
Sbjct: 637 FGLSVSPSQTFNQYFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFT 696

Query: 121 LTIRGSVSQSVVSASLVWTNGVHTVRSPITITTL 155
           LT+RGS+   VVSASLVW++GVH VRSPITIT+L
Sbjct: 697 LTVRGSIKGFVVSASLVWSDGVHYVRSPITITSL 730

[Close]

BLAST of Bhi06G000883 vs. Swiss-Prot
Match: sp|Q9STF7|SBT46_ARATH (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.1e-27
Identity = 66/154 (42.86%), Postives = 100/154 (64.94%), Query Frame = 0


Query: 1   HVNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRVW-DLNYP 60
           HV+P+ A+ PGLVY+AN+SD++ FLCG  Y+   +R I+GD  +CT      +  +LNYP
Sbjct: 582 HVDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYP 641

Query: 61  SFGLSVSRSQTFSQYFTRTLTSVVSQAWTYRAMISAPQGLAITVNPNVLSFNGIGDKKSF 120
           S    VS ++ F   F RT+T+V     TY+A +   + L + V P VLS   + +KKSF
Sbjct: 642 SMSAQVSGTKPFKVTFRRTVTNVGRPNATYKAKVVGSK-LKVKVVPAVLSLKSLYEKKSF 701

Query: 121 TLTIRGS--VSQSVVSASLVWTNGVHTVRSPITI 152
           T+T+ G+   ++++VSA L+W++GVH VRSPI +
Sbjct: 702 TVTVSGAGPKAENLVSAQLIWSDGVHFVRSPIVV 734

[Close]

BLAST of Bhi06G000883 vs. Swiss-Prot
Match: sp|F4JA91|SBT45_ARATH (Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.5e-27
Identity = 66/154 (42.86%), Postives = 99/154 (64.29%), Query Frame = 0


Query: 1   HVNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRV-WDLNYP 60
           HV+P+ A+ PGLVY+AN+SD++ FLCG  Y+   +R I+GD  +CT      +  +LNYP
Sbjct: 582 HVDPITAIHPGLVYEANKSDHIAFLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYP 641

Query: 61  SFGLSVSRSQTFSQYFTRTLTSVVSQAWTYRAMISAPQGLAITVNPNVLSFNGIGDKKSF 120
           S    VS ++ F   F RT+T+V     TY+A +   + L + V P VLS   + +KKSF
Sbjct: 642 SMTAQVSAAKPFKVIFRRTVTNVGRPNATYKAKVVGSK-LKVKVVPAVLSLKSLYEKKSF 701

Query: 121 TLTIRGS--VSQSVVSASLVWTNGVHTVRSPITI 152
           T+T  G+   ++++VSA L+W++GVH VRSPI +
Sbjct: 702 TVTASGAGPKAENLVSAQLIWSDGVHFVRSPIVV 734

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BLAST of Bhi06G000883 vs. Swiss-Prot
Match: sp|Q8L7D2|SBT4C_ARATH (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 5.5e-27
Identity = 66/162 (40.74%), Postives = 104/162 (64.20%), Query Frame = 0


Query: 1   HVNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRVWDLNYPS 60
           HV+P+ A+ PGLVY+ +++D++ FLCG  Y++  ++ I+GD   C+ +N     +LNYPS
Sbjct: 573 HVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPS 632

Query: 61  FGLSVS-RSQTFSQYFTRTLTSVVSQAWTYRAMISAPQG--LAITVNPNVLSFNGIGDKK 120
               +S    TFS  F RTLT+V +   TY++ + A  G  L+I V P+VL F  + +K+
Sbjct: 633 MSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQ 692

Query: 121 SFTLTIRGSVSQSVV--SASLVWTNGVHTVRSPITITTLSLD 158
           SF++T+ GS   S V  SA+L+W++G H VRSPI +  + +D
Sbjct: 693 SFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVYIMVVD 734

[Close]

BLAST of Bhi06G000883 vs. Swiss-Prot
Match: sp|O23357|SBT42_ARATH (Subtilisin-like protease SBT4.2 OS=Arabidopsis thaliana OX=3702 GN=SBT4.2 PE=3 SV=3)

HSP 1 Score: 118.2 bits (295), Expect = 8.0e-26
Identity = 65/155 (41.94%), Postives = 93/155 (60.00%), Query Frame = 0


Query: 2   VNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRVWDLNYPSF 61
           VNP  AV PGLVY+  + DY+  LC   YS+  +  I G    C+ ++   + +LNYPS 
Sbjct: 571 VNPTVAVDPGLVYEIAKEDYLNMLCSLDYSSQGISTIAGGTFTCSEQSKLTMRNLNYPSM 630

Query: 62  GLSVSRSQTFSQYFTRTLTSVVSQAWTYRAMISAPQGLAITVNPNVLSFNGIGDKKSFTL 121
              VS S +    F+RT+T+V  +  TY+A +S    L+I V P  LSF   G+KKSFT+
Sbjct: 631 SAKVSASSSSDITFSRTVTNVGEKGSTYKAKLSGNPKLSIKVEPATLSFKAPGEKKSFTV 690

Query: 122 TIRG---SVSQSVVSASLVWTNGVHTVRSPITITT 154
           T+ G   +   ++VSASL+W++G H VRSPI + T
Sbjct: 691 TVSGKSLAGISNIVSASLIWSDGSHNVRSPIVVYT 725

[Close]

BLAST of Bhi06G000883 vs. TAIR10
Match: AT3G46850.1 (Subtilase family protein)

HSP 1 Score: 124.4 bits (311), Expect = 6.2e-29
Identity = 66/154 (42.86%), Postives = 100/154 (64.94%), Query Frame = 0


Query: 1   HVNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRVW-DLNYP 60
           HV+P+ A+ PGLVY+AN+SD++ FLCG  Y+   +R I+GD  +CT      +  +LNYP
Sbjct: 582 HVDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYP 641

Query: 61  SFGLSVSRSQTFSQYFTRTLTSVVSQAWTYRAMISAPQGLAITVNPNVLSFNGIGDKKSF 120
           S    VS ++ F   F RT+T+V     TY+A +   + L + V P VLS   + +KKSF
Sbjct: 642 SMSAQVSGTKPFKVTFRRTVTNVGRPNATYKAKVVGSK-LKVKVVPAVLSLKSLYEKKSF 701

Query: 121 TLTIRGS--VSQSVVSASLVWTNGVHTVRSPITI 152
           T+T+ G+   ++++VSA L+W++GVH VRSPI +
Sbjct: 702 TVTVSGAGPKAENLVSAQLIWSDGVHFVRSPIVV 734

[Close]

BLAST of Bhi06G000883 vs. TAIR10
Match: AT3G46840.1 (Subtilase family protein)

HSP 1 Score: 123.2 bits (308), Expect = 1.4e-28
Identity = 66/154 (42.86%), Postives = 99/154 (64.29%), Query Frame = 0


Query: 1   HVNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRV-WDLNYP 60
           HV+P+ A+ PGLVY+AN+SD++ FLCG  Y+   +R I+GD  +CT      +  +LNYP
Sbjct: 582 HVDPITAIHPGLVYEANKSDHIAFLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYP 641

Query: 61  SFGLSVSRSQTFSQYFTRTLTSVVSQAWTYRAMISAPQGLAITVNPNVLSFNGIGDKKSF 120
           S    VS ++ F   F RT+T+V     TY+A +   + L + V P VLS   + +KKSF
Sbjct: 642 SMTAQVSAAKPFKVIFRRTVTNVGRPNATYKAKVVGSK-LKVKVVPAVLSLKSLYEKKSF 701

Query: 121 TLTIRGS--VSQSVVSASLVWTNGVHTVRSPITI 152
           T+T  G+   ++++VSA L+W++GVH VRSPI +
Sbjct: 702 TVTASGAGPKAENLVSAQLIWSDGVHFVRSPIVV 734

[Close]

BLAST of Bhi06G000883 vs. TAIR10
Match: AT5G59090.1 (subtilase 4.12)

HSP 1 Score: 122.1 bits (305), Expect = 3.1e-28
Identity = 66/162 (40.74%), Postives = 104/162 (64.20%), Query Frame = 0


Query: 1   HVNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRVWDLNYPS 60
           HV+P+ A+ PGLVY+ +++D++ FLCG  Y++  ++ I+GD   C+ +N     +LNYPS
Sbjct: 573 HVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPS 632

Query: 61  FGLSVS-RSQTFSQYFTRTLTSVVSQAWTYRAMISAPQG--LAITVNPNVLSFNGIGDKK 120
               +S    TFS  F RTLT+V +   TY++ + A  G  L+I V P+VL F  + +K+
Sbjct: 633 MSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQ 692

Query: 121 SFTLTIRGSVSQSVV--SASLVWTNGVHTVRSPITITTLSLD 158
           SF++T+ GS   S V  SA+L+W++G H VRSPI +  + +D
Sbjct: 693 SFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVYIMVVD 734

[Close]

BLAST of Bhi06G000883 vs. TAIR10
Match: AT5G59110.1 (subtilisin-like serine protease-related)

HSP 1 Score: 119.0 bits (297), Expect = 2.6e-27
Identity = 64/156 (41.03%), Postives = 98/156 (62.82%), Query Frame = 0


Query: 1   HVNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRVWDLNYPS 60
           HV+P+ A  PGLVY+ +++D++ FLCG  Y+ + +  I G+   CT  N     +LNYPS
Sbjct: 11  HVDPIAATNPGLVYEMDKADHIAFLCGLNYTADTLALIAGETITCTKENKTLPRNLNYPS 70

Query: 61  FGLSVSRSQ-TFSQYFTRTLTSVVSQAWTYRAMISAPQG--LAITVNPNVLSFNGIGDKK 120
               + RS+ + +  F RT+T+V +   TY++ +   QG  L + V P+VLSF  + +KK
Sbjct: 71  MSAQLRRSESSLTVTFNRTVTNVGTPNSTYKSKVVLNQGSKLNVKVTPSVLSFKTVSEKK 130

Query: 121 SFTLTIRGSVSQSVV--SASLVWTNGVHTVRSPITI 152
           SFT+T+ GS S   +  SA+L+W++G H VRSPI I
Sbjct: 131 SFTVTVTGSDSDPKLPSSANLIWSDGTHNVRSPIVI 166

[Close]

BLAST of Bhi06G000883 vs. TAIR10
Match: AT4G15040.1 (Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 118.2 bits (295), Expect = 4.4e-27
Identity = 65/155 (41.94%), Postives = 93/155 (60.00%), Query Frame = 0


Query: 2   VNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRVWDLNYPSF 61
           VNP  AV PGLVY+  + DY+  LC   YS+  +  I G    C+ ++   + +LNYPS 
Sbjct: 533 VNPTVAVDPGLVYEIAKEDYLNMLCSLDYSSQGISTIAGGTFTCSEQSKLTMRNLNYPSM 592

Query: 62  GLSVSRSQTFSQYFTRTLTSVVSQAWTYRAMISAPQGLAITVNPNVLSFNGIGDKKSFTL 121
              VS S +    F+RT+T+V  +  TY+A +S    L+I V P  LSF   G+KKSFT+
Sbjct: 593 SAKVSASSSSDITFSRTVTNVGEKGSTYKAKLSGNPKLSIKVEPATLSFKAPGEKKSFTV 652

Query: 122 TIRG---SVSQSVVSASLVWTNGVHTVRSPITITT 154
           T+ G   +   ++VSASL+W++G H VRSPI + T
Sbjct: 653 TVSGKSLAGISNIVSASLIWSDGSHNVRSPIVVYT 687

[Close]

BLAST of Bhi06G000883 vs. TrEMBL
Match: tr|A0A1S3CFD6|A0A1S3CFD6_CUCME (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500254 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 3.4e-66
Identity = 126/154 (81.82%), Postives = 141/154 (91.56%), Query Frame = 0


Query: 1   HVNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRVWDLNYPS 60
           HVNPLKAVRPGLVYDANESDYVKFLCGQGY+TNMVR IT D  ACT+ NIGRVWDLNYPS
Sbjct: 575 HVNPLKAVRPGLVYDANESDYVKFLCGQGYTTNMVRSITNDNSACTASNIGRVWDLNYPS 634

Query: 61  FGLSVSRSQTFSQYFTRTLTSVVSQAWTYRAMISAPQGLAITVNPNVLSFNGIGDKKSFT 120
           FGLSVSRSQTF+QYFTR LT+V SQA TYRA IS+PQGL ITVNP VLSFNGIGD+KSFT
Sbjct: 635 FGLSVSRSQTFNQYFTRILTNVASQASTYRAAISSPQGLTITVNPTVLSFNGIGDRKSFT 694

Query: 121 LTIRGSVSQSVVSASLVWTNGVHTVRSPITITTL 155
           LT++G++ +SVVSASLVW +GVH+VRSPIT+T+L
Sbjct: 695 LTVKGTIKESVVSASLVWFDGVHSVRSPITVTSL 728

[Close]

BLAST of Bhi06G000883 vs. TrEMBL
Match: tr|A0A1S3CF86|A0A1S3CF86_CUCME (LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=4 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 5.9e-66
Identity = 127/154 (82.47%), Postives = 139/154 (90.26%), Query Frame = 0


Query: 1   HVNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRVWDLNYPS 60
           HVNPLKAVRPGLVYDANESDYVKFLCGQGY+T  VRRITGDY ACT  N GRVWDLNYPS
Sbjct: 577 HVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPS 636

Query: 61  FGLSVSRSQTFSQYFTRTLTSVVSQAWTYRAMISAPQGLAITVNPNVLSFNGIGDKKSFT 120
           FGLSVS S+TF+QYF RTLTSV  QA TYRAMISAPQGL I+VNPNVLSFNG+GD+KSFT
Sbjct: 637 FGLSVSPSKTFNQYFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFT 696

Query: 121 LTIRGSVSQSVVSASLVWTNGVHTVRSPITITTL 155
           LT+RGS+   VVSASLVW++GVH+VRSPITIT+L
Sbjct: 697 LTVRGSIKGFVVSASLVWSDGVHSVRSPITITSL 730

[Close]

BLAST of Bhi06G000883 vs. TrEMBL
Match: tr|K7NBW1|K7NBW1_SIRGR (Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 3.7e-60
Identity = 119/154 (77.27%), Postives = 136/154 (88.31%), Query Frame = 0


Query: 1   HVNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRVWDLNYPS 60
           HVNPLKAV PGLVYDA+ESDYVKFLCG+GY+T MVR  TGD  ACTS NIGRVWDLNYPS
Sbjct: 581 HVNPLKAVDPGLVYDASESDYVKFLCGEGYTTAMVRSTTGDNSACTSGNIGRVWDLNYPS 640

Query: 61  FGLSVSRSQTFSQYFTRTLTSVVSQAWTYRAMISAPQGLAITVNPNVLSFNGIGDKKSFT 120
           F LS+SRSQT +Q F RTLT+VVS A TYRA ISAPQGL+I+VNP+VLSFNGIGD+KSFT
Sbjct: 641 FALSISRSQTANQSFRRTLTNVVSGASTYRASISAPQGLSISVNPSVLSFNGIGDQKSFT 700

Query: 121 LTIRGSVSQSVVSASLVWTNGVHTVRSPITITTL 155
           LT+RG+VSQ++VSASLVW++G H VRSPIT+  L
Sbjct: 701 LTVRGTVSQAIVSASLVWSDGSHNVRSPITVYVL 734

[Close]

BLAST of Bhi06G000883 vs. TrEMBL
Match: tr|A0A1S4E3D9|A0A1S4E3D9_CUCME (LOW QUALITY PROTEIN: cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500257 PE=4 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.1e-59
Identity = 115/154 (74.68%), Postives = 131/154 (85.06%), Query Frame = 0


Query: 1   HVNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRVWDLNYPS 60
           HVNP +A+RPGLVYDANE DY+KFLCGQGY+  MV  IT    AC S NIGRVWDLNYPS
Sbjct: 575 HVNPKRAIRPGLVYDANEIDYIKFLCGQGYTNGMVEIITSYEDACNSSNIGRVWDLNYPS 634

Query: 61  FGLSVSRSQTFSQYFTRTLTSVVSQAWTYRAMISAPQGLAITVNPNVLSFNGIGDKKSFT 120
           FGLSVS S+TF QYF RTLTSV SQA  Y+AMISAP+GL ITVNPNVLSFNGIGDKKSF 
Sbjct: 635 FGLSVSHSKTFKQYFRRTLTSVASQASKYKAMISAPRGLVITVNPNVLSFNGIGDKKSFK 694

Query: 121 LTIRGSVSQSVVSASLVWTNGVHTVRSPITITTL 155
           L +RG++ +S+VSASLVW++GVH+VRSPITI +L
Sbjct: 695 LKVRGTIKESIVSASLVWSDGVHSVRSPITINSL 728

[Close]

BLAST of Bhi06G000883 vs. TrEMBL
Match: tr|A0A0A0KLR4|A0A0A0KLR4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G187880 PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.5e-53
Identity = 105/152 (69.08%), Postives = 128/152 (84.21%), Query Frame = 0


Query: 1   HVNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRVWDLNYPS 60
           H+NPL+AV PGL+YDA ESDYV+FLCGQGY+T MVRR++GD   CT  N GRVWDLNYPS
Sbjct: 547 HINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVWDLNYPS 606

Query: 61  FGLSVSRSQTFSQYFTRTLTSVVSQAWTYRA-MISAPQGLAITVNPNVLSFNGIGDKKSF 120
           F LS + SQ+F+Q+F RT+T+V S+  TYRA ++  P+GL+ITVNP VLSFN IG KKSF
Sbjct: 607 FALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAIGQKKSF 666

Query: 121 TLTIRGSVSQSVVSASLVWTNGVHTVRSPITI 152
           TLTIRGS+SQS+VSASLVW++G H VRSPIT+
Sbjct: 667 TLTIRGSISQSIVSASLVWSDGHHNVRSPITV 698

[Close]

BLAST of Bhi06G000883 vs. NCBI nr
Match: 3VTA_A (Chain A, Crystal Structure Of Cucumisin, A Subtilisin-like Endoprotease From Cucumis Melo L >3VTA_B Chain B, Crystal Structure Of Cucumisin, A Subtilisin-like Endoprotease From Cucumis Melo L >4YN3_A Chain A, Crystal Structure Of Cucumisin Complex With Pro-peptide)

HSP 1 Score: 261.5 bits (667), Expect = 1.8e-66
Identity = 129/154 (83.77%), Postives = 139/154 (90.26%), Query Frame = 0


Query: 1   HVNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRVWDLNYPS 60
           HVNPLKAVRPGLVYDANESDYVKFLCGQGY+T  VRRITGDY ACTS N GRVWDLNYPS
Sbjct: 467 HVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPS 526

Query: 61  FGLSVSRSQTFSQYFTRTLTSVVSQAWTYRAMISAPQGLAITVNPNVLSFNGIGDKKSFT 120
           FGLSVS SQTF+QYF RTLTSV  QA TYRAMISAPQGL I+VNPNVLSFNG+GD+KSFT
Sbjct: 527 FGLSVSPSQTFNQYFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFT 586

Query: 121 LTIRGSVSQSVVSASLVWTNGVHTVRSPITITTL 155
           LT+RGS+   VVSASLVW++GVH VRSPITIT+L
Sbjct: 587 LTVRGSIKGFVVSASLVWSDGVHYVRSPITITSL 620

[Close]

BLAST of Bhi06G000883 vs. NCBI nr
Match: Q39547.1 (RecName: Full=Cucumisin; AltName: Allergen=Cuc m 1; Flags: Precursor >BAA06905.1 pre-pro-cucumisin [Cucumis melo])

HSP 1 Score: 261.5 bits (667), Expect = 1.8e-66
Identity = 129/154 (83.77%), Postives = 139/154 (90.26%), Query Frame = 0


Query: 1   HVNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRVWDLNYPS 60
           HVNPLKAVRPGLVYDANESDYVKFLCGQGY+T  VRRITGDY ACTS N GRVWDLNYPS
Sbjct: 577 HVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPS 636

Query: 61  FGLSVSRSQTFSQYFTRTLTSVVSQAWTYRAMISAPQGLAITVNPNVLSFNGIGDKKSFT 120
           FGLSVS SQTF+QYF RTLTSV  QA TYRAMISAPQGL I+VNPNVLSFNG+GD+KSFT
Sbjct: 637 FGLSVSPSQTFNQYFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFT 696

Query: 121 LTIRGSVSQSVVSASLVWTNGVHTVRSPITITTL 155
           LT+RGS+   VVSASLVW++GVH VRSPITIT+L
Sbjct: 697 LTVRGSIKGFVVSASLVWSDGVHYVRSPITITSL 730

[Close]

BLAST of Bhi06G000883 vs. NCBI nr
Match: XP_008461722.1 (PREDICTED: cucumisin-like [Cucumis melo])

HSP 1 Score: 260.0 bits (663), Expect = 5.2e-66
Identity = 126/154 (81.82%), Postives = 141/154 (91.56%), Query Frame = 0


Query: 1   HVNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRVWDLNYPS 60
           HVNPLKAVRPGLVYDANESDYVKFLCGQGY+TNMVR IT D  ACT+ NIGRVWDLNYPS
Sbjct: 575 HVNPLKAVRPGLVYDANESDYVKFLCGQGYTTNMVRSITNDNSACTASNIGRVWDLNYPS 634

Query: 61  FGLSVSRSQTFSQYFTRTLTSVVSQAWTYRAMISAPQGLAITVNPNVLSFNGIGDKKSFT 120
           FGLSVSRSQTF+QYFTR LT+V SQA TYRA IS+PQGL ITVNP VLSFNGIGD+KSFT
Sbjct: 635 FGLSVSRSQTFNQYFTRILTNVASQASTYRAAISSPQGLTITVNPTVLSFNGIGDRKSFT 694

Query: 121 LTIRGSVSQSVVSASLVWTNGVHTVRSPITITTL 155
           LT++G++ +SVVSASLVW +GVH+VRSPIT+T+L
Sbjct: 695 LTVKGTIKESVVSASLVWFDGVHSVRSPITVTSL 728

[Close]

BLAST of Bhi06G000883 vs. NCBI nr
Match: XP_008461721.1 (PREDICTED: LOW QUALITY PROTEIN: cucumisin [Cucumis melo])

HSP 1 Score: 259.2 bits (661), Expect = 8.9e-66
Identity = 127/154 (82.47%), Postives = 139/154 (90.26%), Query Frame = 0


Query: 1   HVNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRVWDLNYPS 60
           HVNPLKAVRPGLVYDANESDYVKFLCGQGY+T  VRRITGDY ACT  N GRVWDLNYPS
Sbjct: 577 HVNPLKAVRPGLVYDANESDYVKFLCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPS 636

Query: 61  FGLSVSRSQTFSQYFTRTLTSVVSQAWTYRAMISAPQGLAITVNPNVLSFNGIGDKKSFT 120
           FGLSVS S+TF+QYF RTLTSV  QA TYRAMISAPQGL I+VNPNVLSFNG+GD+KSFT
Sbjct: 637 FGLSVSPSKTFNQYFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFT 696

Query: 121 LTIRGSVSQSVVSASLVWTNGVHTVRSPITITTL 155
           LT+RGS+   VVSASLVW++GVH+VRSPITIT+L
Sbjct: 697 LTVRGSIKGFVVSASLVWSDGVHSVRSPITITSL 730

[Close]

BLAST of Bhi06G000883 vs. NCBI nr
Match: AEM42989.1 (cucumisin [Siraitia grosvenorii])

HSP 1 Score: 240.0 bits (611), Expect = 5.6e-60
Identity = 119/154 (77.27%), Postives = 136/154 (88.31%), Query Frame = 0


Query: 1   HVNPLKAVRPGLVYDANESDYVKFLCGQGYSTNMVRRITGDYGACTSRNIGRVWDLNYPS 60
           HVNPLKAV PGLVYDA+ESDYVKFLCG+GY+T MVR  TGD  ACTS NIGRVWDLNYPS
Sbjct: 581 HVNPLKAVDPGLVYDASESDYVKFLCGEGYTTAMVRSTTGDNSACTSGNIGRVWDLNYPS 640

Query: 61  FGLSVSRSQTFSQYFTRTLTSVVSQAWTYRAMISAPQGLAITVNPNVLSFNGIGDKKSFT 120
           F LS+SRSQT +Q F RTLT+VVS A TYRA ISAPQGL+I+VNP+VLSFNGIGD+KSFT
Sbjct: 641 FALSISRSQTANQSFRRTLTNVVSGASTYRASISAPQGLSISVNPSVLSFNGIGDQKSFT 700

Query: 121 LTIRGSVSQSVVSASLVWTNGVHTVRSPITITTL 155
           LT+RG+VSQ++VSASLVW++G H VRSPIT+  L
Sbjct: 701 LTVRGTVSQAIVSASLVWSDGSHNVRSPITVYVL 734

The following BLAST results are available for this feature:

BLAST of Bhi06G000883 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q39547\|CUCM1_CUCME	5.9e-69	83.77	Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
sp\|Q9STF7\|SBT46_ARATH	1.1e-27	42.86	Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S...	[more]
sp\|F4JA91\|SBT45_ARATH	2.5e-27	42.86	Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 S...	[more]
sp\|Q8L7D2\|SBT4C_ARATH	5.5e-27	40.74	Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2...	[more]
sp\|O23357\|SBT42_ARATH	8.0e-26	41.94	Subtilisin-like protease SBT4.2 OS=Arabidopsis thaliana OX=3702 GN=SBT4.2 PE=3 S...	[more]

BLAST of Bhi06G000883 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G46850.1	6.2e-29	42.86	Subtilase family protein	[more]
AT3G46840.1	1.4e-28	42.86	Subtilase family protein	[more]
AT5G59090.1	3.1e-28	40.74	subtilase 4.12	[more]
AT5G59110.1	2.6e-27	41.03	subtilisin-like serine protease-related	[more]
AT4G15040.1	4.4e-27	41.94	Subtilisin-like serine endopeptidase family protein	[more]

BLAST of Bhi06G000883 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3CFD6\|A0A1S3CFD6_CUCME	3.4e-66	81.82	cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500254 PE=4 SV=1	[more]
tr\|A0A1S3CF86\|A0A1S3CF86_CUCME	5.9e-66	82.47	LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=4 SV=1	[more]
tr\|K7NBW1\|K7NBW1_SIRGR	3.7e-60	77.27	Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1	[more]
tr\|A0A1S4E3D9\|A0A1S4E3D9_CUCME	3.1e-59	74.68	LOW QUALITY PROTEIN: cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500257 PE=4...	[more]
tr\|A0A0A0KLR4\|A0A0A0KLR4_CUCSA	1.5e-53	69.08	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G187880 PE=4 SV=1	[more]

BLAST of Bhi06G000883 vs. NCBI nr


Match Name	E-value	Identity	Description
3VTA_A	1.8e-66	83.77	Chain A, Crystal Structure Of Cucumisin, A Subtilisin-like Endoprotease From Cuc...	[more]
Q39547.1	1.8e-66	83.77	RecName: Full=Cucumisin; AltName: Allergen=Cuc m 1; Flags: Precursor >BAA06905.1...	[more]
XP_008461722.1	5.2e-66	81.82	PREDICTED: cucumisin-like [Cucumis melo]	[more]
XP_008461721.1	8.9e-66	82.47	PREDICTED: LOW QUALITY PROTEIN: cucumisin [Cucumis melo]	[more]
AEM42989.1	5.6e-60	77.27	cucumisin [Siraitia grosvenorii]	[more]

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004252	serine-type endopeptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi06M000883	Bhi06M000883	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:2.60.40.2310		coord: 25..155 e-value: 1.4E-43 score: 149.7
None	No IPR available	PANTHER	PTHR10795:SF348	SUBTILISIN-LIKE PROTEASE SBT4.1	coord: 1..150
None	No IPR available	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 1..150

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Bhi06G000883	MELO3C023911	Melon (DHL92) v3.5.1	mewgoB057

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi06G000883	Cucumber (Gy14) v2	cgybwgoB441
Bhi06G000883	Cucurbita pepo (Zucchini)	cpewgoB0009
Bhi06G000883	Bottle gourd (USVL1VR-Ls)	lsiwgoB353
Bhi06G000883	Melon (DHL92) v3.6.1	medwgoB056
Bhi06G000883	Watermelon (Charleston Gray)	wcgwgoB559