Bhi05G000648 (gene) Wax gourd

BLAST of Bhi05G000648 vs. Swiss-Prot
Match: sp|Q9SA65|CAP4_ARATH (Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=At1g03050 PE=2 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 2.6e-173
Identity = 344/600 (57.33%), Postives = 417/600 (69.50%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKV-GSSTSLSDLEVAIVKATRHEEYPAEERHVREILSL 60
           M  SK +RA+GAVKD+TS+GLAKV G S SLS+L+VAIVKATRHEE+PAEE+++REILSL
Sbjct: 1   MGSSKFKRAIGAVKDQTSVGLAKVNGRSASLSELDVAIVKATRHEEFPAEEKYIREILSL 60

Query: 61  TCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRF 120
           T YSR+YI+ACV+TLSRRLNKTK WTVALKTL+LIQRL+ EGD AYEQEIFF+TRRGTR 
Sbjct: 61  TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDXXXXXXXXXXX 180
           LNMSDFRD S+SNSWDYSAFVRT+ALYLDERL+FRMQ+R GKR  +              
Sbjct: 121 LNMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRMQARHGKRGVY-------------- 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTG 240
                                     R+ PI +M +EQIF R QHLQQLL+RFLACRPTG
Sbjct: 181 --CVGGEADEEEQDQAAADLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTG 240

Query: 241 AAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFD 300
            A+NNRVV+VALYPIV+ESFQIYYD+TEIMGILI+RFMEL++ D +KVY+IFCRV KQF+
Sbjct: 241 NARNNRVVIVALYPIVKESFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFE 300

Query: 301 ELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEI 360
           EL+ FYSWC+ +GIARSSEYP++EKIT KKL++MDEFI+DKSAL    T + K+      
Sbjct: 301 ELDQFYSWCKNMGIARSSEYPEIEKITQKKLDLMDEFIRDKSALE--HTKQSKSVKSEAX 360

Query: 361 TEEVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKDENPLIGDLLNL 420
                                                                +GDLL+L
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXMGDLLDL 420

Query: 421 GEEHAMIREENADKLALALFDGIGPVTDSSSVKTLP-WQAFNDDSQDWETTLVQSASNLS 480
           G  +     +  D LALALFD  GP    S  ++ P W+AF DDS DWET LVQ+A+NLS
Sbjct: 421 GNTNGGEAGQAGDSLALALFD--GPYASGSGSESGPGWEAFKDDSADWETALVQTATNLS 480

Query: 481 NQRADLGGGFDMLLLDGMYKQTTMMSTM-AGSGYGVSGSASSMALGSAGRPA--MLALPA 540
            Q+++LGGGFDMLLL+GMY+   + + +   + YG SGSASSMA GSAGRPA  MLALPA
Sbjct: 481 GQKSELGGGFDMLLLNGMYQHGAVNAAVKTSTAYGASGSASSMAFGSAGRPAATMLALPA 540

Query: 541 PPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQ 596
           P T     G  +S V  DPFAAS+ VAPP YVQM++ME+KQ++L+EEQ+MW QY+RDGRQ
Sbjct: 541 PSTANGNAGNINSPVPMDPFAASLEVAPPAYVQMNDMEKKQRMLMEEQMMWDQYSRDGRQ 580

BLAST of Bhi05G000648 vs. Swiss-Prot
Match: sp|Q8GX47|CAP3_ARATH (Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=At4g02650 PE=2 SV=2)

HSP 1 Score: 557.4 bits (1435), Expect = 2.0e-157
Identity = 339/599 (56.59%), Postives = 419/599 (69.95%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGS-STSLSDLEVAIVKATRHEEYPAEERHVREILSL 60
           M  SK++RA+GAVKD+TS+GLAKVG  S+SL++LE+A+VKATRH++YPAE++++REIL L
Sbjct: 1   MGSSKLKRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCL 60

Query: 61  TCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRF 120
           T YSR Y+SACV TLSRRLNKTKNW+VALKTL+LIQRL+++GD AYEQEIFF+TRRGTR 
Sbjct: 61  TSYSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDXXXXXXXXXXX 180
           LNMSDFRD SQS+SWDYSAFVRT+ALYLDERL++RMQ R           XXXXXXXXXX
Sbjct: 121 LNMSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGR-----------XXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTG 240
           XXXXXXXXXXXXX             ++ P+ +M +E+IF+R QHLQQLL+RFLACRPTG
Sbjct: 181 XXXXXXXXXXXXXGTSNDIRSKAIVVKSKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTG 240

Query: 241 AAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFD 300
            AKNNRVV+VA+YPIV+ESFQ+YY+ITEIMG+LI+RFMEL++HD +KVYEIFCRV KQFD
Sbjct: 241 NAKNNRVVIVAMYPIVKESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFD 300

Query: 301 ELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEI 360
           EL+ FY WC+ + +ARSSEYP++EKIT KKL++MDEFI+DKSALA  Q            
Sbjct: 301 ELDPFYGWCKNMAVARSSEYPELEKITQKKLDLMDEFIRDKSALA-AQXXXXXXXXXXXX 360

Query: 361 TEEVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKDENPLIGDLLNL 420
                                                                 GDLL+L
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGDLLDL 420

Query: 421 GEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWETTLVQSASNLSN 480
            +E  +      D LALALFDG   V  + S     W+AFND+S DWET LV+SA+ LS 
Sbjct: 421 TDEAGVTAGTVGDSLALALFDG---VVGTESASGPGWEAFNDNSADWETDLVRSATRLSG 480

Query: 481 QRADLGGGFDMLLLDGMYKQTTMMSTM-AGSGYGVSGSA--SSMALGSAGRPAMLALPAP 540
           Q+++LGGGFD LLLDGMY+   + + +   + YG SGSA             +MLALPAP
Sbjct: 481 QKSELGGGFDTLLLDGMYQYGAVNAAVKTSTAYGSSGSAXXXXXXXXXXXXXSMLALPAP 540

Query: 541 PTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQ 596
           P    G    +S V+ DPFAAS+ VAPP YVQM++ME+KQ+LL+EEQ+MW QY R GRQ
Sbjct: 541 PPTANGN--RNSPVMVDPFAASLEVAPPAYVQMNDMEKKQRLLMEEQIMWDQYNRSGRQ 582

BLAST of Bhi05G000648 vs. Swiss-Prot
Match: sp|Q8S9J8|CAP1_ARATH (Probable clathrin assembly protein At4g32285 OS=Arabidopsis thaliana OX=3702 GN=At4g32285 PE=1 SV=2)

HSP 1 Score: 439.1 bits (1128), Expect = 7.9e-122
Identity = 257/627 (40.99%), Postives = 371/627 (59.17%), Query Frame = 0

Query: 6   IRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLTCYSRA 65
           +R+A+G VKD+TSIG+AKV S+ +  DLEVAIVKAT H++  + ++++REILSLT  SR 
Sbjct: 5   MRKAIGVVKDQTSIGIAKVASNMA-PDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 64

Query: 66  YISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFLNMSDF 125
           Y+ ACV ++SRRL KT++W VALK LML+ RL++EGDP +++EI ++TRRGTR LNMSDF
Sbjct: 65  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 124

Query: 126 RDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGK------------------RSAFGL 185
           RD + S+SWD+SAFVRT+A YLD+RLE  +  RRG+                  RS    
Sbjct: 125 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNXXXXXXXXXXXXDDGYNRSRDDF 184

Query: 186 DXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRATPIRDMTSEQIFSRTQHLQQ 245
                                                   TP+R+MT E+IF +  HLQ+
Sbjct: 185 RSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFGKMGHLQR 244

Query: 246 LLERFLACRPTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKV 305
           LL+RFL+CRPTG AKN+R++L+A+YP+V+ESF++Y DI E++ +L+D+F ++   DCVK 
Sbjct: 245 LLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDMEYTDCVKA 304

Query: 306 YEIFCRVGKQFDELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQ 365
           ++ +    KQ DEL  FY WC+  G+ARSSEYP+V++IT K LE ++EF++D++  A+  
Sbjct: 305 FDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDRAKRAKSP 364

Query: 366 TGRGKNQLQIEITEEVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKK 425
               + +++         EE +DMN IKALPP                    + +D++  
Sbjct: 365 E---RKEIEAPPAPAPPVEEPVDMNEIKALPPXXXXXXXXXXXXXXXXXXXXVTDDLVNL 424

Query: 426 DENPLIGDLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQ--- 485
            E+ + GD             +  +K ALALF   GP  ++       W+AF+ D+    
Sbjct: 425 REDDVSGD-------------DQGNKFALALF--AGPPANNGK-----WEAFSSDNNVTS 484

Query: 486 ------------DWETTLVQSASNLSNQRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYG 545
                       DWE  LV++ASNL +Q+A +GGG D LLL+GMY Q  +   ++ S   
Sbjct: 485 AWQNPAAELGKADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSEL- 544

Query: 546 VSGSASSMALGSAGR--PAMLALPAPPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMS 598
             GS+SS+AL   G+    +LALPAP    +        V  DPFAAS+ + PP YVQM+
Sbjct: 545 TGGSSSSVALPLPGKVNSHILALPAPDGTVQ-------KVNQDPFAASLTIPPPSYVQMA 599

BLAST of Bhi05G000648 vs. Swiss-Prot
Match: sp|Q8LF20|CAP2_ARATH (Putative clathrin assembly protein At2g25430 OS=Arabidopsis thaliana OX=3702 GN=At2g25430 PE=1 SV=2)

HSP 1 Score: 414.8 bits (1065), Expect = 1.6e-114
Identity = 289/645 (44.81%), Postives = 381/645 (59.07%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPS IR+A+GAVKD+TSIG+AKV S+ +  DLEVAIVKAT H++ PA E+++REIL+LT
Sbjct: 1   MAPS-IRKAIGAVKDQTSIGIAKVASNMA-PDLEVAIVKATSHDDDPASEKYIREILNLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120
             SR YI ACV ++SRRL+KT++W VALK LML+ RL++EGDP +++EI +STRRGTR L
Sbjct: 61  SLSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRML 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSA---------------- 180
           NMSDFRD + S+SWD+SAFVRT+A YLD+RLE  +  R+   S                 
Sbjct: 121 NMSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSXXXXXXXXXXXXXX 180

Query: 181 ----------------------FGLDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240
                                     XXXXXXX                XXXXXXXXXXX
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYGGVPKRSRSYGDMTEXXXXXXXXXXX 240

Query: 241 XXXRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGAAKNNRVVLVALYPIVRESFQIY 300
           XXX  TP+R+MT E+IF +  HLQ+LL+RFL+ RPTG AKN+R++L+ALYP+VRESF++Y
Sbjct: 241 XXXXXTPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESFKLY 300

Query: 301 YDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDELELFYSWCRTIGIARSSEYPDV 360
            DI E++ +L+D+F ++   DCVK ++ +    KQ DEL  FY+WC+  G+ARSSEYP+V
Sbjct: 301 ADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEYPEV 360

Query: 361 EKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEITEEVKKEEEIDMNSIKALPPPED 420
           ++IT K LE ++EF++D++        RGK+          +KE E              
Sbjct: 361 QRITSKLLETLEEFVRDRAK-------RGKS--------PERKEIEAXXXXXXXXXXXXX 420

Query: 421 FKEEDITAIEVKEEENEIKEDVIKKDENPLIGDLLNLGEEHAMIREENADKLALALFDGI 480
                                           DL+NL E+  +  ++  +K ALALF G 
Sbjct: 421 XXXXXXXXXXXXXXXXXXXXXXXXXXXPQFTEDLVNLREDE-VTADDQGNKFALALFAG- 480

Query: 481 GPVTD------SSSVKTLPWQ--AFNDDSQDWETTLVQSASNLSNQRADLGGGFDMLLLD 540
            P  +      SS+  T  WQ  A      DWE  LV++ SNL  Q A LGGGFD LLL+
Sbjct: 481 PPGNNGKWEAFSSNGVTSAWQNPAAEPGKADWELALVETTSNLEKQTAALGGGFDNLLLN 540

Query: 541 GMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRP--AMLALPAPPTITEGGGVSSSSVVS 598
           GMY Q  M+     +     GSASS+AL   G+    +LALPAP    E        V  
Sbjct: 541 GMYDQ-GMVRQHVSTSQLTGGSASSVALPLPGKTNNQVLALPAPDGTVE-------KVNQ 600

BLAST of Bhi05G000648 vs. Swiss-Prot
Match: sp|Q9ZVN6|AP180_ARATH (Clathrin coat assembly protein AP180 OS=Arabidopsis thaliana OX=3702 GN=AP180 PE=1 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.4e-65
Identity = 136/349 (38.97%), Postives = 208/349 (59.60%), Query Frame = 0

Query: 3   PSKIRRALGAVKDKTSIGLAKVGSSTS----LSDLEVAIVKATRH-EEYPAEERHVREIL 62
           PSK+++A+GAVKD+TSI LAKV +  +    L+ LEVAI+KAT H EE P ++R V EIL
Sbjct: 2   PSKLKKAIGAVKDQTSISLAKVANGATGGGDLTTLEVAILKATSHDEEVPIDDRLVTEIL 61

Query: 63  SLTCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGT 122
            +    +++ ++C   + RR+ +T+NW VALK+L+L+ R+  +GDP + +E+  + +RG 
Sbjct: 62  GIISSKKSHAASCAAAIGRRIGRTRNWIVALKSLVLVLRIFQDGDPYFPREVLHAMKRGA 121

Query: 123 RFLNMSDFRDTSQSNSWDYSAFVRTFALYLDERLEF----RMQSRRGKRSAFGLDXXXXX 182
           + LN+S FRD S S  WD++AFVRTFALYLDERL+     ++Q R   R   G       
Sbjct: 122 KILNLSSFRDDSNSCPWDFTAFVRTFALYLDERLDCFLTGKLQRRYTNREQTG------- 181

Query: 183 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRATPIRDMTSEQIFSRTQHLQQLLERFL 242
                                               +RDM    +  +  + Q+LL+R +
Sbjct: 182 ------------RISTNSTTRSRFNPKAGIKSHEPAVRDMKPVMLLDKITYWQKLLDRAI 241

Query: 243 ACRPTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCR 302
           A RPTG AK NR+V ++LY +++ESF +Y DI++ + +L+D F  L    C+  ++   R
Sbjct: 242 ATRPTGDAKANRLVKMSLYAVMQESFDLYRDISDGLALLLDSFFHLQYQSCINAFQACVR 301

Query: 303 VGKQFDELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSA 343
             KQF+EL  FY   ++IGI R+SEYP ++KI+ + LE + EF+KD+S+
Sbjct: 302 ASKQFEELNAFYDLSKSIGIGRTSEYPSIQKISLELLETLQEFLKDQSS 331

BLAST of Bhi05G000648 vs. TAIR10
Match: AT1G03050.1 (ENTH/ANTH/VHS superfamily protein)

HSP 1 Score: 610.1 bits (1572), Expect = 1.4e-174
Identity = 344/600 (57.33%), Postives = 417/600 (69.50%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKV-GSSTSLSDLEVAIVKATRHEEYPAEERHVREILSL 60
           M  SK +RA+GAVKD+TS+GLAKV G S SLS+L+VAIVKATRHEE+PAEE+++REILSL
Sbjct: 1   MGSSKFKRAIGAVKDQTSVGLAKVNGRSASLSELDVAIVKATRHEEFPAEEKYIREILSL 60

Query: 61  TCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRF 120
           T YSR+YI+ACV+TLSRRLNKTK WTVALKTL+LIQRL+ EGD AYEQEIFF+TRRGTR 
Sbjct: 61  TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDXXXXXXXXXXX 180
           LNMSDFRD S+SNSWDYSAFVRT+ALYLDERL+FRMQ+R GKR  +              
Sbjct: 121 LNMSDFRDVSRSNSWDYSAFVRTYALYLDERLDFRMQARHGKRGVY-------------- 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTG 240
                                     R+ PI +M +EQIF R QHLQQLL+RFLACRPTG
Sbjct: 181 --CVGGEADEEEQDQAAADLSTAIVVRSQPIAEMKTEQIFIRIQHLQQLLDRFLACRPTG 240

Query: 241 AAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFD 300
            A+NNRVV+VALYPIV+ESFQIYYD+TEIMGILI+RFMEL++ D +KVY+IFCRV KQF+
Sbjct: 241 NARNNRVVIVALYPIVKESFQIYYDVTEIMGILIERFMELDIPDSIKVYDIFCRVSKQFE 300

Query: 301 ELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEI 360
           EL+ FYSWC+ +GIARSSEYP++EKIT KKL++MDEFI+DKSAL    T + K+      
Sbjct: 301 ELDQFYSWCKNMGIARSSEYPEIEKITQKKLDLMDEFIRDKSALE--HTKQSKSVKSEAX 360

Query: 361 TEEVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKDENPLIGDLLNL 420
                                                                +GDLL+L
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXMGDLLDL 420

Query: 421 GEEHAMIREENADKLALALFDGIGPVTDSSSVKTLP-WQAFNDDSQDWETTLVQSASNLS 480
           G  +     +  D LALALFD  GP    S  ++ P W+AF DDS DWET LVQ+A+NLS
Sbjct: 421 GNTNGGEAGQAGDSLALALFD--GPYASGSGSESGPGWEAFKDDSADWETALVQTATNLS 480

Query: 481 NQRADLGGGFDMLLLDGMYKQTTMMSTM-AGSGYGVSGSASSMALGSAGRPA--MLALPA 540
            Q+++LGGGFDMLLL+GMY+   + + +   + YG SGSASSMA GSAGRPA  MLALPA
Sbjct: 481 GQKSELGGGFDMLLLNGMYQHGAVNAAVKTSTAYGASGSASSMAFGSAGRPAATMLALPA 540

Query: 541 PPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQ 596
           P T     G  +S V  DPFAAS+ VAPP YVQM++ME+KQ++L+EEQ+MW QY+RDGRQ
Sbjct: 541 PSTANGNAGNINSPVPMDPFAASLEVAPPAYVQMNDMEKKQRMLMEEQMMWDQYSRDGRQ 580

BLAST of Bhi05G000648 vs. TAIR10
Match: AT4G02650.1 (ENTH/ANTH/VHS superfamily protein)

HSP 1 Score: 557.4 bits (1435), Expect = 1.1e-158
Identity = 339/599 (56.59%), Postives = 419/599 (69.95%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGS-STSLSDLEVAIVKATRHEEYPAEERHVREILSL 60
           M  SK++RA+GAVKD+TS+GLAKVG  S+SL++LE+A+VKATRH++YPAE++++REIL L
Sbjct: 1   MGSSKLKRAIGAVKDQTSVGLAKVGGRSSSLTELEIAVVKATRHDDYPAEDKYIREILCL 60

Query: 61  TCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRF 120
           T YSR Y+SACV TLSRRLNKTKNW+VALKTL+LIQRL+++GD AYEQEIFF+TRRGTR 
Sbjct: 61  TSYSRNYVSACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGTRL 120

Query: 121 LNMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDXXXXXXXXXXX 180
           LNMSDFRD SQS+SWDYSAFVRT+ALYLDERL++RMQ R           XXXXXXXXXX
Sbjct: 121 LNMSDFRDASQSDSWDYSAFVRTYALYLDERLDYRMQGR-----------XXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTG 240
           XXXXXXXXXXXXX             ++ P+ +M +E+IF+R QHLQQLL+RFLACRPTG
Sbjct: 181 XXXXXXXXXXXXXGTSNDIRSKAIVVKSKPVAEMKTEKIFNRVQHLQQLLDRFLACRPTG 240

Query: 241 AAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFD 300
            AKNNRVV+VA+YPIV+ESFQ+YY+ITEIMG+LI+RFMEL++HD +KVYEIFCRV KQFD
Sbjct: 241 NAKNNRVVIVAMYPIVKESFQLYYNITEIMGVLIERFMELDIHDSIKVYEIFCRVSKQFD 300

Query: 301 ELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEI 360
           EL+ FY WC+ + +ARSSEYP++EKIT KKL++MDEFI+DKSALA  Q            
Sbjct: 301 ELDPFYGWCKNMAVARSSEYPELEKITQKKLDLMDEFIRDKSALA-AQXXXXXXXXXXXX 360

Query: 361 TEEVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKDENPLIGDLLNL 420
                                                                 GDLL+L
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGDLLDL 420

Query: 421 GEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWETTLVQSASNLSN 480
            +E  +      D LALALFDG   V  + S     W+AFND+S DWET LV+SA+ LS 
Sbjct: 421 TDEAGVTAGTVGDSLALALFDG---VVGTESASGPGWEAFNDNSADWETDLVRSATRLSG 480

Query: 481 QRADLGGGFDMLLLDGMYKQTTMMSTM-AGSGYGVSGSA--SSMALGSAGRPAMLALPAP 540
           Q+++LGGGFD LLLDGMY+   + + +   + YG SGSA             +MLALPAP
Sbjct: 481 QKSELGGGFDTLLLDGMYQYGAVNAAVKTSTAYGSSGSAXXXXXXXXXXXXXSMLALPAP 540

Query: 541 PTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQ 596
           P    G    +S V+ DPFAAS+ VAPP YVQM++ME+KQ+LL+EEQ+MW QY R GRQ
Sbjct: 541 PPTANGN--RNSPVMVDPFAASLEVAPPAYVQMNDMEKKQRLLMEEQIMWDQYNRSGRQ 582

BLAST of Bhi05G000648 vs. TAIR10
Match: AT4G32285.1 (ENTH/ANTH/VHS superfamily protein)

HSP 1 Score: 439.1 bits (1128), Expect = 4.4e-123
Identity = 257/627 (40.99%), Postives = 371/627 (59.17%), Query Frame = 0

Query: 6   IRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLTCYSRA 65
           +R+A+G VKD+TSIG+AKV S+ +  DLEVAIVKAT H++  + ++++REILSLT  SR 
Sbjct: 5   MRKAIGVVKDQTSIGIAKVASNMA-PDLEVAIVKATSHDDDQSSDKYIREILSLTSLSRG 64

Query: 66  YISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFLNMSDF 125
           Y+ ACV ++SRRL KT++W VALK LML+ RL++EGDP +++EI ++TRRGTR LNMSDF
Sbjct: 65  YVHACVTSVSRRLKKTRDWIVALKALMLVHRLLNEGDPLFQEEILYATRRGTRILNMSDF 124

Query: 126 RDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGK------------------RSAFGL 185
           RD + S+SWD+SAFVRT+A YLD+RLE  +  RRG+                  RS    
Sbjct: 125 RDEAHSSSWDHSAFVRTYASYLDQRLELALFERRGRNXXXXXXXXXXXXDDGYNRSRDDF 184

Query: 186 DXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRATPIRDMTSEQIFSRTQHLQQ 245
                                                   TP+R+MT E+IF +  HLQ+
Sbjct: 185 RSPPPRTYDYETGNGFGMPKRSRSFGDVNEIGAREEKKSVTPLREMTPERIFGKMGHLQR 244

Query: 246 LLERFLACRPTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKV 305
           LL+RFL+CRPTG AKN+R++L+A+YP+V+ESF++Y DI E++ +L+D+F ++   DCVK 
Sbjct: 245 LLDRFLSCRPTGLAKNSRMILIAMYPVVKESFRLYADICEVLAVLLDKFFDMEYTDCVKA 304

Query: 306 YEIFCRVGKQFDELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQ 365
           ++ +    KQ DEL  FY WC+  G+ARSSEYP+V++IT K LE ++EF++D++  A+  
Sbjct: 305 FDAYASAAKQIDELIAFYHWCKDTGVARSSEYPEVQRITSKLLETLEEFVRDRAKRAKSP 364

Query: 366 TGRGKNQLQIEITEEVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKK 425
               + +++         EE +DMN IKALPP                    + +D++  
Sbjct: 365 E---RKEIEAPPAPAPPVEEPVDMNEIKALPPXXXXXXXXXXXXXXXXXXXXVTDDLVNL 424

Query: 426 DENPLIGDLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQ--- 485
            E+ + GD             +  +K ALALF   GP  ++       W+AF+ D+    
Sbjct: 425 REDDVSGD-------------DQGNKFALALF--AGPPANNGK-----WEAFSSDNNVTS 484

Query: 486 ------------DWETTLVQSASNLSNQRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYG 545
                       DWE  LV++ASNL +Q+A +GGG D LLL+GMY Q  +   ++ S   
Sbjct: 485 AWQNPAAELGKADWELALVETASNLEHQKAAMGGGLDPLLLNGMYDQGAVRQHVSTSEL- 544

Query: 546 VSGSASSMALGSAGR--PAMLALPAPPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMS 598
             GS+SS+AL   G+    +LALPAP    +        V  DPFAAS+ + PP YVQM+
Sbjct: 545 TGGSSSSVALPLPGKVNSHILALPAPDGTVQ-------KVNQDPFAASLTIPPPSYVQMA 599

BLAST of Bhi05G000648 vs. TAIR10
Match: AT2G25430.1 (epsin N-terminal homology (ENTH) domain-containing protein / clathrin assembly protein-related)

HSP 1 Score: 414.8 bits (1065), Expect = 8.9e-116
Identity = 289/645 (44.81%), Postives = 381/645 (59.07%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPS IR+A+GAVKD+TSIG+AKV S+ +  DLEVAIVKAT H++ PA E+++REIL+LT
Sbjct: 1   MAPS-IRKAIGAVKDQTSIGIAKVASNMA-PDLEVAIVKATSHDDDPASEKYIREILNLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120
             SR YI ACV ++SRRL+KT++W VALK LML+ RL++EGDP +++EI +STRRGTR L
Sbjct: 61  SLSRGYILACVTSVSRRLSKTRDWVVALKALMLVHRLLNEGDPIFQEEILYSTRRGTRML 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSA---------------- 180
           NMSDFRD + S+SWD+SAFVRT+A YLD+RLE  +  R+   S                 
Sbjct: 121 NMSDFRDEAHSSSWDHSAFVRTYAGYLDQRLELALFERKSGVSVNSXXXXXXXXXXXXXX 180

Query: 181 ----------------------FGLDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240
                                     XXXXXXX                XXXXXXXXXXX
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYGGVPKRSRSYGDMTEXXXXXXXXXXX 240

Query: 241 XXXRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGAAKNNRVVLVALYPIVRESFQIY 300
           XXX  TP+R+MT E+IF +  HLQ+LL+RFL+ RPTG AKN+R++L+ALYP+VRESF++Y
Sbjct: 241 XXXXXTPLREMTPERIFGKMGHLQRLLDRFLSLRPTGLAKNSRMILIALYPVVRESFKLY 300

Query: 301 YDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDELELFYSWCRTIGIARSSEYPDV 360
            DI E++ +L+D+F ++   DCVK ++ +    KQ DEL  FY+WC+  G+ARSSEYP+V
Sbjct: 301 ADICEVLAVLLDKFFDMEYSDCVKAFDAYASAAKQIDELIAFYNWCKETGVARSSEYPEV 360

Query: 361 EKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEITEEVKKEEEIDMNSIKALPPPED 420
           ++IT K LE ++EF++D++        RGK+          +KE E              
Sbjct: 361 QRITSKLLETLEEFVRDRAK-------RGKS--------PERKEIEAXXXXXXXXXXXXX 420

Query: 421 FKEEDITAIEVKEEENEIKEDVIKKDENPLIGDLLNLGEEHAMIREENADKLALALFDGI 480
                                           DL+NL E+  +  ++  +K ALALF G 
Sbjct: 421 XXXXXXXXXXXXXXXXXXXXXXXXXXXPQFTEDLVNLREDE-VTADDQGNKFALALFAG- 480

Query: 481 GPVTD------SSSVKTLPWQ--AFNDDSQDWETTLVQSASNLSNQRADLGGGFDMLLLD 540
            P  +      SS+  T  WQ  A      DWE  LV++ SNL  Q A LGGGFD LLL+
Sbjct: 481 PPGNNGKWEAFSSNGVTSAWQNPAAEPGKADWELALVETTSNLEKQTAALGGGFDNLLLN 540

Query: 541 GMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRP--AMLALPAPPTITEGGGVSSSSVVS 598
           GMY Q  M+     +     GSASS+AL   G+    +LALPAP    E        V  
Sbjct: 541 GMYDQ-GMVRQHVSTSQLTGGSASSVALPLPGKTNNQVLALPAPDGTVE-------KVNQ 600

BLAST of Bhi05G000648 vs. TAIR10
Match: AT1G05020.1 (ENTH/ANTH/VHS superfamily protein)

HSP 1 Score: 252.3 bits (643), Expect = 7.6e-67
Identity = 136/349 (38.97%), Postives = 208/349 (59.60%), Query Frame = 0

Query: 3   PSKIRRALGAVKDKTSIGLAKVGSSTS----LSDLEVAIVKATRH-EEYPAEERHVREIL 62
           PSK+++A+GAVKD+TSI LAKV +  +    L+ LEVAI+KAT H EE P ++R V EIL
Sbjct: 2   PSKLKKAIGAVKDQTSISLAKVANGATGGGDLTTLEVAILKATSHDEEVPIDDRLVTEIL 61

Query: 63  SLTCYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGT 122
            +    +++ ++C   + RR+ +T+NW VALK+L+L+ R+  +GDP + +E+  + +RG 
Sbjct: 62  GIISSKKSHAASCAAAIGRRIGRTRNWIVALKSLVLVLRIFQDGDPYFPREVLHAMKRGA 121

Query: 123 RFLNMSDFRDTSQSNSWDYSAFVRTFALYLDERLEF----RMQSRRGKRSAFGLDXXXXX 182
           + LN+S FRD S S  WD++AFVRTFALYLDERL+     ++Q R   R   G       
Sbjct: 122 KILNLSSFRDDSNSCPWDFTAFVRTFALYLDERLDCFLTGKLQRRYTNREQTG------- 181

Query: 183 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRATPIRDMTSEQIFSRTQHLQQLLERFL 242
                                               +RDM    +  +  + Q+LL+R +
Sbjct: 182 ------------RISTNSTTRSRFNPKAGIKSHEPAVRDMKPVMLLDKITYWQKLLDRAI 241

Query: 243 ACRPTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCR 302
           A RPTG AK NR+V ++LY +++ESF +Y DI++ + +L+D F  L    C+  ++   R
Sbjct: 242 ATRPTGDAKANRLVKMSLYAVMQESFDLYRDISDGLALLLDSFFHLQYQSCINAFQACVR 301

Query: 303 VGKQFDELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSA 343
             KQF+EL  FY   ++IGI R+SEYP ++KI+ + LE + EF+KD+S+
Sbjct: 302 ASKQFEELNAFYDLSKSIGIGRTSEYPSIQKISLELLETLQEFLKDQSS 331

BLAST of Bhi05G000648 vs. TrEMBL
Match: tr|A0A0A0K783|A0A0A0K783_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G058550 PE=4 SV=1)

HSP 1 Score: 866.7 bits (2238), Expect = 3.1e-248
Identity = 512/621 (82.45%), Postives = 527/621 (84.86%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLD-------XXXXX 180
           NMSDFRDTSQSNSWDYSAFVRT+ALYLDERLEFRMQSRRG+RSAFGLD       XXXXX
Sbjct: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRATPIRDMTSEQIFSRTQHLQQLLERFL 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX RATP+RDMTSEQIFSRTQHLQQLLERFL
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVRATPMRDMTSEQIFSRTQHLQQLLERFL 240

Query: 241 ACRPTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCR 300
           ACRPTGAAKNNRVVLVALYP+VRESFQIYYDITEIMGILIDRFM+LNV DCVKVYEIFCR
Sbjct: 241 ACRPTGAAKNNRVVLVALYPVVRESFQIYYDITEIMGILIDRFMDLNVQDCVKVYEIFCR 300

Query: 301 VGKQFDELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQT----- 360
           VGKQFDELE+FYSWCRTIGIARS+EYP+VEKITPKKLEVMDEFIKD+SALAQCQ      
Sbjct: 301 VGKQFDELEMFYSWCRTIGIARSAEYPEVEKITPKKLEVMDEFIKDRSALAQCQNDXXXX 360

Query: 361 GRGKNQLQIEITEEVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKD 420
                                                                       
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 420

Query: 421 ENPLIGDLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWET 480
            N ++GDLLNLGEEHAMIRE NADKLA ALFDG GPV   SSVK LPWQAFNDDS DWET
Sbjct: 421 XNSMMGDLLNLGEEHAMIREANADKLATALFDGSGPV--DSSVKALPWQAFNDDSADWET 480

Query: 481 TLVQSASNLSNQRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRP 540
            LVQSAS+L NQ+ADLGGGFDMLLLDGMYKQT + STMAGSGYGVSGSASSMALGSAGRP
Sbjct: 481 ALVQSASHLPNQKADLGGGFDMLLLDGMYKQTAVKSTMAGSGYGVSGSASSMALGSAGRP 540

Query: 541 AMLALPAPPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQ 600
           AMLALPA PT  EG G SSSSVVSDPFAAS+AVAPPPYVQMSEMERKQKLLVEEQLMWQQ
Sbjct: 541 AMLALPA-PTTPEGSGTSSSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQ 600

Query: 601 YARDGRQIQHNPYTGGFTHSY 610
           YARDGRQIQHNPYTGGFTHSY
Sbjct: 601 YARDGRQIQHNPYTGGFTHSY 618

BLAST of Bhi05G000648 vs. TrEMBL
Match: tr|A0A1S3C1P9|A0A1S3C1P9_CUCME (putative clathrin assembly protein At1g03050 OS=Cucumis melo OX=3656 GN=LOC103495455 PE=4 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 5.9e-247
Identity = 509/612 (83.17%), Postives = 523/612 (85.46%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSS SLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDXXXXXXXXXXXX 180
           NMSDFRD S SNSWDYSAFVRT+ALYLDERLEFRMQSRRG+RSAFGLDXXXXXXXXXXXX
Sbjct: 121 NMSDFRDASLSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA 240
           XXXXXXXXXXXXXXXXXXXXXXXX RATPIRDMTS+QIFSRTQHLQQLLERFLACRPTGA
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXIRATPIRDMTSDQIFSRTQHLQQLLERFLACRPTGA 240

Query: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDE 300
           AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMEL + DCVKVYEIFCRVGKQFDE
Sbjct: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELKIQDCVKVYEIFCRVGKQFDE 300

Query: 301 LELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEIT 360
           LELFYSWC+TIGIARSSEYP+VEKITPKKLEVMDEFIKD+SALAQCQ  R K Q Q+EI 
Sbjct: 301 LELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDRSALAQCQKDREKAQPQLEIA 360

Query: 361 E---EVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKDENPLIGDLL 420
           E                                                   N ++GDLL
Sbjct: 361 EXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNSMMGDLL 420

Query: 421 NLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWETTLVQSASNL 480
           NLGEEHAMIREENADKLALALFDG GPV   SSVK LPWQAFND+S DWE+ LVQSASNL
Sbjct: 421 NLGEEHAMIREENADKLALALFDGSGPV--DSSVKALPWQAFNDESADWESALVQSASNL 480

Query: 481 SNQRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRPAMLALPAPP 540
           SNQRADLGGGFDMLLLDGMYKQT + STMAGSGYGVSGSASSMALGSAGRPAMLAL    
Sbjct: 481 SNQRADLGGGFDMLLLDGMYKQTAVKSTMAGSGYGVSGSASSMALGSAGRPAMLAL-XXX 540

Query: 541 TITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQ 600
                       VVSDPFAAS+AVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQ
Sbjct: 541 XXXXXXXXXXXXVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQ 600

Query: 601 HNPYTGGFTHSY 610
           HNPYTGGFTHSY
Sbjct: 601 HNPYTGGFTHSY 609

BLAST of Bhi05G000648 vs. TrEMBL
Match: tr|A0A2N9IF46|A0A2N9IF46_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS50970 PE=4 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 1.0e-214
Identity = 428/623 (68.70%), Postives = 483/623 (77.53%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPS IR+A+GAVKD TSIGLAKVGSS SL+DL+VAIVKATRH++YPAEERH+REILSLT
Sbjct: 1   MAPSTIRKAIGAVKDTTSIGLAKVGSSASLADLDVAIVKATRHDDYPAEERHIREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTK+WTVALKTL LIQRL+SEGDPAYEQEIFFSTRRGTR L
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKDWTVALKTLALIQRLLSEGDPAYEQEIFFSTRRGTRLL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDXXXXXXXXXXXX 180
           NMSDFRDTSQSNSWD+S+FVRT+ LYLDERLEFRMQSRRGKRSAFG +            
Sbjct: 121 NMSDFRDTSQSNSWDHSSFVRTYGLYLDERLEFRMQSRRGKRSAFGFE------------ 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA 240
                         XXXX       RATP+R+M  E+IFSR QHLQQLLERFLACRPTGA
Sbjct: 181 -------------EXXXXHNQAGGQRATPVREMKIEKIFSRMQHLQQLLERFLACRPTGA 240

Query: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDE 300
           +K NRVV+VALYPIV+ESFQIYYDITEIMGILIDRFMEL VHDCVKVYE+FCRVGKQFDE
Sbjct: 241 SKTNRVVIVALYPIVKESFQIYYDITEIMGILIDRFMELEVHDCVKVYEVFCRVGKQFDE 300

Query: 301 LELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEIT 360
           L++FYSWC+ IGIARSSEYP++EKITPKKLEVMDEFI+DKSALAQ +  +    L+ EI 
Sbjct: 301 LDIFYSWCKNIGIARSSEYPELEKITPKKLEVMDEFIRDKSALAQIKQSK---SLKEEIK 360

Query: 361 EEVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEE------NEIKEDVIKKDENPLIG 420
           E  +K  E D+N+IKALPPPE F E     + V+EE+          E  I + E    G
Sbjct: 361 EAEEKNVE-DVNAIKALPPPESFTE----LVPVEEEKIXXXXXXXXXETKITQHE----G 420

Query: 421 DLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWETTLVQSA 480
           DLLNLGE+ A+  EE+ADKLALALFDG   VT+ +   +L WQAFND+S DWE+ LVQSA
Sbjct: 421 DLLNLGED-AITSEEHADKLALALFDGEAQVTNQA--PSLTWQAFNDESADWESDLVQSA 480

Query: 481 SNLSNQRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRPAMLALP 540
           SNLS Q+A LGGGFDMLLLDGMYKQ    + M   GYGVSGSASS+ALGSAGRPAMLALP
Sbjct: 481 SNLSGQKAALGGGFDMLLLDGMYKQAETSAAMTSPGYGVSGSASSVALGSAGRPAMLALP 540

Query: 541 APPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGR 600
           A           SS   +DPFAAS+AVAPP YVQMS+ME+KQKLL++EQLMWQQYA+DG 
Sbjct: 541 AXXXXXXXXXXXSS---ADPFAASLAVAPPAYVQMSDMEKKQKLLMDEQLMWQQYAKDGM 580

Query: 601 QIQ---------HNPYTGGFTHS 609
           Q Q         HNP   G  H+
Sbjct: 601 QGQLGIAKMQQHHNPLQHGRLHT 580

BLAST of Bhi05G000648 vs. TrEMBL
Match: tr|A0A2P4MYT4|A0A2P4MYT4_QUESU (Putative clathrin assembly protein OS=Quercus suber OX=58331 GN=CFP56_08338 PE=4 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 1.7e-214
Identity = 419/619 (67.69%), Postives = 482/619 (77.87%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPS IR+A+GAVKD+TSI LAKVGSS SL DLEVAIVKATRH+EYPAEERH+REILSLT
Sbjct: 1   MAPSTIRKAIGAVKDQTSISLAKVGSSNSLGDLEVAIVKATRHDEYPAEERHIREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120
           CYSRAYIS+CVNTLSRRL+KTKNW VALKTLMLIQRL+S+GDPAYEQEIFFSTRRGTR L
Sbjct: 61  CYSRAYISSCVNTLSRRLSKTKNWIVALKTLMLIQRLLSDGDPAYEQEIFFSTRRGTRLL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDXXXXXXXXXXXX 180
           N+SDFRD S SNSWDYS+F+RT+ALYLDERLEFRMQSRRGKRSAF LD            
Sbjct: 121 NLSDFRDISHSNSWDYSSFIRTYALYLDERLEFRMQSRRGKRSAFALD------------ 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA 240
                                    RATP+R+M +E IFSR QHLQQLLER LACRPTG 
Sbjct: 181 -----------EDDEEASQHNQAPPRATPVREMKNELIFSRMQHLQQLLERLLACRPTGE 240

Query: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDE 300
           AKNNRVV+VALYPIV+ESFQIYYDITEIMGIL+DRFME  V DCVK++E+FCRVGKQFDE
Sbjct: 241 AKNNRVVIVALYPIVKESFQIYYDITEIMGILVDRFMEQEVQDCVKIHEMFCRVGKQFDE 300

Query: 301 LELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEIT 360
           L++FY+W ++IGIARSSEYP++EKIT KKLE+MDEFI+DKSAL QC+    K++ Q E  
Sbjct: 301 LDMFYAWSKSIGIARSSEYPELEKITRKKLEIMDEFIRDKSALEQCK----KSKPQQEEQ 360

Query: 361 EEVKKEEEI-DMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKDENPLIGDLLNL 420
           +EV  EE + DMN+IKALPPPE F E  +  +E K+E   IK++  +       GDLLNL
Sbjct: 361 KEVPVEENVEDMNAIKALPPPEGFVETPV--VEEKKEVEPIKKEEKETKITQQEGDLLNL 420

Query: 421 GEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWETTLVQSASNLSN 480
           G E  +  EE+ADKLALALFDG    T+ +    L W+AFND++ DWE+TLVQSASNLS 
Sbjct: 421 G-EGTITSEEHADKLALALFDGGAQPTNQA--PALAWEAFNDENADWESTLVQSASNLSG 480

Query: 481 QRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRPAMLALPAPPTI 540
           Q+  L GGFDMLLLDGMYKQ+   +   G G+GVSGSASS+AL SAGRPAMLALPAPPT 
Sbjct: 481 QKPSLPGGFDMLLLDGMYKQSETNAANQGPGFGVSGSASSVALDSAGRPAMLALPAPPT- 540

Query: 541 TEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQ---- 600
            EG    SSS  +DPFAAS+AVAPPPYVQMS+ME+KQKLLV+EQLMWQQYA+DG Q    
Sbjct: 541 PEGSSAMSSS--ADPFAASLAVAPPPYVQMSDMEKKQKLLVDEQLMWQQYAKDGMQGQLG 584

Query: 601 ----IQHNPYT-GGFTHSY 610
                QHNPY  GG+T SY
Sbjct: 601 IAKMQQHNPYNMGGYTQSY 584

BLAST of Bhi05G000648 vs. TrEMBL
Match: tr|A0A2I4GE20|A0A2I4GE20_9ROSI (putative clathrin assembly protein At4g02650 OS=Juglans regia OX=51240 GN=LOC109007077 PE=4 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 4.4e-210
Identity = 408/618 (66.02%), Postives = 469/618 (75.89%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           M  SKIR+ALGAVKD TSIGLAKVGSS SL+DL++AIVKATRH+EYPAEERH+REILSLT
Sbjct: 1   MGASKIRKALGAVKDTTSIGLAKVGSSASLADLDIAIVKATRHDEYPAEERHIREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120
           CYSRA+ISACVNTLSRRLNKT+NW VALKTLM+IQRL+ EGDPAYEQE+FF+TR+GTR L
Sbjct: 61  CYSRAFISACVNTLSRRLNKTRNWIVALKTLMVIQRLLLEGDPAYEQEVFFATRQGTRLL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDXXXXXXXXXXXX 180
           NMSDFRD+SQSNSWDYS+FVRT+ALYLDERLEFRMQSRRGKRS    D            
Sbjct: 121 NMSDFRDSSQSNSWDYSSFVRTYALYLDERLEFRMQSRRGKRSGHSFD------------ 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA 240
                                    RATP+ +M +E IFSR QHLQQLLERFLACRPTG 
Sbjct: 181 --------HEDHDHDQEAMANQAGPRATPVCEMRTELIFSRVQHLQQLLERFLACRPTGV 240

Query: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDE 300
           AK NRVV+VALYPIV+ESF IYYDITEI+GIL+DRFMEL V DCV+VYEIFCRVGKQFDE
Sbjct: 241 AKTNRVVIVALYPIVKESFHIYYDITEILGILVDRFMELEVIDCVRVYEIFCRVGKQFDE 300

Query: 301 LELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEIT 360
           LE+FY+WC + GI RSSE+P++EKIT KKLE+MDEFI++KSAL Q    + + +      
Sbjct: 301 LEMFYAWCNSTGIGRSSEFPELEKITGKKLELMDEFIREKSALVQSIKPKSQEEXXXXXX 360

Query: 361 EEVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKDENPLIGDLLNLG 420
             V ++ E DMN+IKALPPPE F E                   + + E    GDLLNLG
Sbjct: 361 XXVVEKNEEDMNAIKALPPPEGFTEAXXXXXXXXXXXXXXXXXKVTQQE----GDLLNLG 420

Query: 421 EEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWETTLVQSASNLSNQ 480
           ++ A+  EE+ADKLALALFDG+ P+  ++ V  L W+AFND+  DWETTLVQSASNLS Q
Sbjct: 421 DD-AITSEEHADKLALALFDGVVPLA-TNEVPALTWEAFNDEKSDWETTLVQSASNLSGQ 480

Query: 481 RADLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVS-GSASSMALGSAGRPAMLALPAPPTI 540
           +A LGGGFDMLLLDGMYKQ+   + MAG  YG S GSASS+ALGSAG+PAMLALPAPPT 
Sbjct: 481 KATLGGGFDMLLLDGMYKQSATTTAMAGPDYGTSTGSASSVALGSAGKPAMLALPAPPT- 540

Query: 541 TEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQ-- 600
            EGG  S++SV  DPFAAS+AV  P YVQMSEME+KQKLLVEEQLMWQQYARDG Q Q  
Sbjct: 541 PEGGSTSTTSV--DPFAASLAVPAPAYVQMSEMEKKQKLLVEEQLMWQQYARDGMQGQIG 589

Query: 601 ------HNPYT-GGFTHS 609
                 HNPY  GG+THS
Sbjct: 601 MTKIQPHNPYNMGGYTHS 589

BLAST of Bhi05G000648 vs. NCBI nr
Match: XP_011658768.1 (PREDICTED: putative clathrin assembly protein At1g03050 [Cucumis sativus] >KGN43681.1 hypothetical protein Csa_7G058550 [Cucumis sativus])

HSP 1 Score: 866.7 bits (2238), Expect = 4.7e-248
Identity = 512/621 (82.45%), Postives = 527/621 (84.86%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLD-------XXXXX 180
           NMSDFRDTSQSNSWDYSAFVRT+ALYLDERLEFRMQSRRG+RSAFGLD       XXXXX
Sbjct: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRATPIRDMTSEQIFSRTQHLQQLLERFL 240
           XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX RATP+RDMTSEQIFSRTQHLQQLLERFL
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVRATPMRDMTSEQIFSRTQHLQQLLERFL 240

Query: 241 ACRPTGAAKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCR 300
           ACRPTGAAKNNRVVLVALYP+VRESFQIYYDITEIMGILIDRFM+LNV DCVKVYEIFCR
Sbjct: 241 ACRPTGAAKNNRVVLVALYPVVRESFQIYYDITEIMGILIDRFMDLNVQDCVKVYEIFCR 300

Query: 301 VGKQFDELELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQT----- 360
           VGKQFDELE+FYSWCRTIGIARS+EYP+VEKITPKKLEVMDEFIKD+SALAQCQ      
Sbjct: 301 VGKQFDELEMFYSWCRTIGIARSAEYPEVEKITPKKLEVMDEFIKDRSALAQCQNDXXXX 360

Query: 361 GRGKNQLQIEITEEVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKD 420
                                                                       
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 420

Query: 421 ENPLIGDLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWET 480
            N ++GDLLNLGEEHAMIRE NADKLA ALFDG GPV   SSVK LPWQAFNDDS DWET
Sbjct: 421 XNSMMGDLLNLGEEHAMIREANADKLATALFDGSGPV--DSSVKALPWQAFNDDSADWET 480

Query: 481 TLVQSASNLSNQRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRP 540
            LVQSAS+L NQ+ADLGGGFDMLLLDGMYKQT + STMAGSGYGVSGSASSMALGSAGRP
Sbjct: 481 ALVQSASHLPNQKADLGGGFDMLLLDGMYKQTAVKSTMAGSGYGVSGSASSMALGSAGRP 540

Query: 541 AMLALPAPPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQ 600
           AMLALPA PT  EG G SSSSVVSDPFAAS+AVAPPPYVQMSEMERKQKLLVEEQLMWQQ
Sbjct: 541 AMLALPA-PTTPEGSGTSSSSVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQ 600

Query: 601 YARDGRQIQHNPYTGGFTHSY 610
           YARDGRQIQHNPYTGGFTHSY
Sbjct: 601 YARDGRQIQHNPYTGGFTHSY 618

BLAST of Bhi05G000648 vs. NCBI nr
Match: XP_023511651.1 (putative clathrin assembly protein At1g03050 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 863.6 bits (2230), Expect = 4.0e-247
Identity = 499/619 (80.61%), Postives = 537/619 (86.75%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSS SLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120
           CYS+AYISACVNTLSRRLNKTK+WTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSKAYISACVNTLSRRLNKTKDWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDXXXXXXXXXXXX 180
           NMSDFRDTSQSNSWDYSAFVRT+ALYLDERLE+RMQSRRG+RSAFGL     XXXXXXXX
Sbjct: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEYRMQSRRGRRSAFGL---LEXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA 240
           XXXXXXXXXXXXXXXXXXXXXXXX RATP+ +MT+EQ+FS+TQHLQQLLERFLACRPTG+
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXIRATPLHEMTTEQLFSKTQHLQQLLERFLACRPTGS 240

Query: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDE 300
              NRVVLVALYPIV+ESFQIYYDITE MGILIDRFM+L + DCV+VYE+FCRVGKQFDE
Sbjct: 241 FHINRVVLVALYPIVKESFQIYYDITETMGILIDRFMDLKIRDCVRVYEVFCRVGKQFDE 300

Query: 301 LELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKN------- 360
           LE+FYSWCR+IGIARS+EYP+VEKITPKKLEVMDEFIKDKSALAQC    G         
Sbjct: 301 LEMFYSWCRSIGIARSTEYPEVEKITPKKLEVMDEFIKDKSALAQCLRNEGDEVXXXXXX 360

Query: 361 -QLQIEITEEVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEE--NEIKEDVIKKDEN 420
                               SIKALPPPE F+EE  TA+ V EE   NE+K    + + N
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXSIKALPPPESFEEEQ-TALLVNEENETNEMKAKEKRDEHN 420

Query: 421 PLIGDLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWETTL 480
           P++GD LNL EEH M REENA+KLA ALFDG  P+ D +SV  LPW+AF+DD+ DWET L
Sbjct: 421 PMMGDFLNLDEEHQMTREENAEKLAFALFDGSAPIID-ASVPALPWEAFSDDAPDWETAL 480

Query: 481 VQSASNLSNQRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRPAM 540
           VQSASNLSNQR DLGGGFDMLLLDGMYKQTT MSTMAGSGYGVSGSASSMALGSAGRPAM
Sbjct: 481 VQSASNLSNQRTDLGGGFDMLLLDGMYKQTTTMSTMAGSGYGVSGSASSMALGSAGRPAM 540

Query: 541 LALPAPPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYA 600
           LALPAP T +EGG  SSSSV+SDPFAAS+AVAPP YVQMSEMERK+KLLVEEQLMWQQYA
Sbjct: 541 LALPAPST-SEGGVASSSSVMSDPFAASVAVAPPAYVQMSEMERKKKLLVEEQLMWQQYA 600

Query: 601 RDGRQIQHNPYTGGFTHSY 610
           RDGRQIQ NPYTGG+THSY
Sbjct: 601 RDGRQIQPNPYTGGYTHSY 613

BLAST of Bhi05G000648 vs. NCBI nr
Match: XP_008455244.1 (PREDICTED: putative clathrin assembly protein At1g03050 [Cucumis melo])

HSP 1 Score: 862.4 bits (2227), Expect = 8.9e-247
Identity = 509/612 (83.17%), Postives = 523/612 (85.46%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSS SLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120
           CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDXXXXXXXXXXXX 180
           NMSDFRD S SNSWDYSAFVRT+ALYLDERLEFRMQSRRG+RSAFGLDXXXXXXXXXXXX
Sbjct: 121 NMSDFRDASLSNSWDYSAFVRTYALYLDERLEFRMQSRRGRRSAFGLDXXXXXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA 240
           XXXXXXXXXXXXXXXXXXXXXXXX RATPIRDMTS+QIFSRTQHLQQLLERFLACRPTGA
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXIRATPIRDMTSDQIFSRTQHLQQLLERFLACRPTGA 240

Query: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDE 300
           AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMEL + DCVKVYEIFCRVGKQFDE
Sbjct: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELKIQDCVKVYEIFCRVGKQFDE 300

Query: 301 LELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQIEIT 360
           LELFYSWC+TIGIARSSEYP+VEKITPKKLEVMDEFIKD+SALAQCQ  R K Q Q+EI 
Sbjct: 301 LELFYSWCKTIGIARSSEYPEVEKITPKKLEVMDEFIKDRSALAQCQKDREKAQPQLEIA 360

Query: 361 E---EVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKDENPLIGDLL 420
           E                                                   N ++GDLL
Sbjct: 361 EXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNSMMGDLL 420

Query: 421 NLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWETTLVQSASNL 480
           NLGEEHAMIREENADKLALALFDG GPV   SSVK LPWQAFND+S DWE+ LVQSASNL
Sbjct: 421 NLGEEHAMIREENADKLALALFDGSGPV--DSSVKALPWQAFNDESADWESALVQSASNL 480

Query: 481 SNQRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRPAMLALPAPP 540
           SNQRADLGGGFDMLLLDGMYKQT + STMAGSGYGVSGSASSMALGSAGRPAMLAL    
Sbjct: 481 SNQRADLGGGFDMLLLDGMYKQTAVKSTMAGSGYGVSGSASSMALGSAGRPAMLAL-XXX 540

Query: 541 TITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQ 600
                       VVSDPFAAS+AVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQ
Sbjct: 541 XXXXXXXXXXXXVVSDPFAASVAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDGRQIQ 600

Query: 601 HNPYTGGFTHSY 610
           HNPYTGGFTHSY
Sbjct: 601 HNPYTGGFTHSY 609

BLAST of Bhi05G000648 vs. NCBI nr
Match: XP_022967586.1 (putative clathrin assembly protein At1g03050 [Cucurbita maxima])

HSP 1 Score: 860.9 bits (2223), Expect = 2.6e-246
Identity = 498/616 (80.84%), Postives = 539/616 (87.50%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSS SLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120
           CYS+AYISACVNTLSRRLNKTK+WTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSKAYISACVNTLSRRLNKTKDWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDXXXXXXXXXXXX 180
           NMSDFRDTS+SNSWDYSAFVRT+ALYLDERLE+RMQSRRG+RSAFGL    XXXXXXXXX
Sbjct: 121 NMSDFRDTSESNSWDYSAFVRTYALYLDERLEYRMQSRRGRRSAFGLQ---XXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA 240
           XXXXXXXXXXXXXXXXXXXXXXXXXR TP+ +MT+EQIFS+TQHLQQLLERFLACRPTGA
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXXXXRVTPLHEMTTEQIFSKTQHLQQLLERFLACRPTGA 240

Query: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDE 300
           AK NRVVLVALYPIV+ESFQIYYDITE MGILIDRFM+L + DCV+VYEIFCRVGKQFDE
Sbjct: 241 AKINRVVLVALYPIVKESFQIYYDITETMGILIDRFMDLKIRDCVRVYEIFCRVGKQFDE 300

Query: 301 LELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKN------Q 360
           LE+FYSWCR+IGIARS+EYP+VEKITPKKLEVMDEFIKDKSALAQC    G         
Sbjct: 301 LEIFYSWCRSIGIARSTEYPEVEKITPKKLEVMDEFIKDKSALAQCLRNGGDEVXXXXXX 360

Query: 361 LQIEITEEVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEENEIKEDVIKKDE-NPLI 420
                             SIKALPPPE FKEE I  +  +E +N   +   K+DE +P++
Sbjct: 361 XXXXXXXXXXXXXXXXXXSIKALPPPESFKEEQIALLVNEENQNNEMKAKEKRDEHHPMM 420

Query: 421 GDLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWETTLVQS 480
           GD LNL EEH M REENA++LA ALFDG  P+ D +SV  LPW+AF+DD+ DWET LVQS
Sbjct: 421 GDFLNLDEEHQMTREENAEQLAFALFDGSAPIID-ASVPALPWEAFSDDTPDWETALVQS 480

Query: 481 ASNLSNQRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGRPAMLAL 540
           AS+LSNQR DLGGGFDMLLLDGMYKQTT M T+AGSGYGVSGSASSMALGSAGRPAMLAL
Sbjct: 481 ASSLSNQRTDLGGGFDMLLLDGMYKQTTRMPTVAGSGYGVSGSASSMALGSAGRPAMLAL 540

Query: 541 PAPPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQQYARDG 600
           PAP T +EGG VSSSSV+SDPFAAS+AVAPP YVQ+SEMERKQKLLVEE+LMWQQYARDG
Sbjct: 541 PAPST-SEGGVVSSSSVMSDPFAASVAVAPPAYVQISEMERKQKLLVEEKLMWQQYARDG 600

Query: 601 RQIQHNPYTGGFTHSY 610
           RQIQ NPYTGG+THSY
Sbjct: 601 RQIQPNPYTGGYTHSY 611

BLAST of Bhi05G000648 vs. NCBI nr
Match: XP_022963838.1 (putative clathrin assembly protein At1g03050 isoform X1 [Cucurbita moschata])

HSP 1 Score: 854.0 bits (2205), Expect = 3.2e-244
Identity = 497/622 (79.90%), Postives = 534/622 (85.85%), Query Frame = 0

Query: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSTSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60
           MAPSKIRRALGAVKDKTSIGLAKVGSS SLSDLEVAIVKATRHEEYPAEERHVREILSLT
Sbjct: 1   MAPSKIRRALGAVKDKTSIGLAKVGSSNSLSDLEVAIVKATRHEEYPAEERHVREILSLT 60

Query: 61  CYSRAYISACVNTLSRRLNKTKNWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120
           CYS+AYISACVNTLSRRLNKTK+WTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL
Sbjct: 61  CYSKAYISACVNTLSRRLNKTKDWTVALKTLMLIQRLVSEGDPAYEQEIFFSTRRGTRFL 120

Query: 121 NMSDFRDTSQSNSWDYSAFVRTFALYLDERLEFRMQSRRGKRSAFGLDXXXXXXXXXXXX 180
           NMSDFRDTSQSNSWDYSAFVRT+ALYLDERLE+RMQSRRG+RSAFGL     XXXXXXXX
Sbjct: 121 NMSDFRDTSQSNSWDYSAFVRTYALYLDERLEYRMQSRRGRRSAFGL---LEXXXXXXXX 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXRATPIRDMTSEQIFSRTQHLQQLLERFLACRPTGA 240
           XXXXXXXXXXXXXXXXXXXXXX   RATP+ +MT+EQIFS+TQHLQQLLERFLACRPTGA
Sbjct: 181 XXXXXXXXXXXXXXXXXXXXXXIMIRATPLHEMTTEQIFSKTQHLQQLLERFLACRPTGA 240

Query: 241 AKNNRVVLVALYPIVRESFQIYYDITEIMGILIDRFMELNVHDCVKVYEIFCRVGKQFDE 300
           AK NRVVLVALYPIV+ESFQIYYDITE MGILIDRFM+L + DCV+VYEIFCRVGKQFDE
Sbjct: 241 AKVNRVVLVALYPIVKESFQIYYDITETMGILIDRFMDLKIRDCVRVYEIFCRVGKQFDE 300

Query: 301 LELFYSWCRTIGIARSSEYPDVEKITPKKLEVMDEFIKDKSALAQCQTGRGKNQLQ---- 360
           LE+FYSWCR IGIARS+EYP+VEKITPKKLEVMDEFIKDKSALAQC     +N+++    
Sbjct: 301 LEMFYSWCRNIGIARSTEYPEVEKITPKKLEVMDEFIKDKSALAQCL----RNEVEXXXX 360

Query: 361 -------IEITEEVKKEEEIDMNSIKALPPPEDFKEEDITAIEVKEEE--NEIKEDVIKK 420
                                   IKALPPPE F E   TA+ V EE   NE+K      
Sbjct: 361 XXXXXXXXXXXXXXXXXXXXXXXXIKALPPPESF-EXXQTALLVNEENETNEMKAKEKSD 420

Query: 421 DENPLIGDLLNLGEEHAMIREENADKLALALFDGIGPVTDSSSVKTLPWQAFNDDSQDWE 480
           +  P++GD LNL EEH M REENA+KLA ALFDG  P+ D +SV  LPW+AF+DD+ DWE
Sbjct: 421 EHYPMMGDFLNLDEEHQMTREENAEKLAFALFDGSAPIID-ASVPALPWEAFSDDAPDWE 480

Query: 481 TTLVQSASNLSNQRADLGGGFDMLLLDGMYKQTTMMSTMAGSGYGVSGSASSMALGSAGR 540
           T LVQSASNLSNQR DLGGGFDMLLLDGMYKQTT MSTMAGSGYGVSGSASSMALGSAGR
Sbjct: 481 TALVQSASNLSNQRTDLGGGFDMLLLDGMYKQTTTMSTMAGSGYGVSGSASSMALGSAGR 540

Query: 541 PAMLALPAPPTITEGGGVSSSSVVSDPFAASMAVAPPPYVQMSEMERKQKLLVEEQLMWQ 600
           PA+LALPAP T +EG   SSSSV+SDPFAAS+AVAPP YVQMSEMERK+KLLVEEQLMWQ
Sbjct: 541 PALLALPAPST-SEGSVASSSSVMSDPFAASVAVAPPAYVQMSEMERKKKLLVEEQLMWQ 600

Query: 601 QYARDGRQIQHNPYTGGFTHSY 610
           QYARDGRQIQ NPYTGG+THSY
Sbjct: 601 QYARDGRQIQPNPYTGGYTHSY 612