Bhi04G001653 (gene) Wax gourd

BLAST of Bhi04G001653 vs. Swiss-Prot
Match: sp|U3KRF8|SGSL_TRIAN (Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2)

HSP 1 Score: 455.7 bits (1171), Expect = 7.3e-127
Identity = 236/476 (49.58%), Postives = 321/476 (67.44%), Query Frame = 0

Query: 22  NQELPLSSSDLLVDGYKNFINTIRERLTKNTSKLYDIPILKNSLPPTEPFITINLKNANN 81
           N++  L  S+     YK FI  +RE L   T +LY IP+LK+SL  +  F  + L +  +
Sbjct: 1   NEQANLRLSEANSGTYKTFIGRVREELGSETYRLYGIPVLKHSLSNSNRFYLLTLTSNQD 60

Query: 82  ETIVLAIDVVNLGVVGYR--SNNTSYVFFNAPPEASEIVFPSTCRVLLRFDSDYESIEKA 141
           E+I LAIDV ++  V Y+   ++ SY F NAP  A   +F  T + +L FD+ ++S+E A
Sbjct: 61  ESITLAIDVEDMVAVAYQPAGSHESYFFLNAPQIAFHTLFTDTHQNVLNFDNTFKSLENA 120

Query: 142 SGISRLQTLLGTDPLNSAISNLFHYHQESLPQSFLVILQMVLEGVKFKFIEQSVIKSLKY 201
           +G +R   +LG DPL+ AISNLF+   + LP SFLVI+QMVLE  KF+FIEQSV  S K 
Sbjct: 121 AGTTRQTIVLGVDPLDFAISNLFNADPKLLPLSFLVIIQMVLEASKFRFIEQSVAYSFKN 180

Query: 202 GYNFKPSLAMISLQDNWAKLSLQIQASTSLQGLFGEAINFYDSNNRIIQVDSIYYPIIIT 261
              F P LA++SL+DNW+++SLQIQASTSLQGLFG  +  Y+SNN +I+VDSIYYPII+ 
Sbjct: 181 EKTFLPDLAIVSLEDNWSEISLQIQASTSLQGLFGSVVELYNSNNELIEVDSIYYPIILA 240

Query: 262 NIALQLYQCNVSTNFIRMPSITSDSPCYIQTQTSSISGREGFCIDATRGIQYDGNPIISS 321
           N+ALQLY C VS         T D+ C ++T+T+ ISGR+  C+D    +  DG+ +I  
Sbjct: 241 NVALQLYHCQVS---------TGDNECLVETRTTRISGRDALCVDVAGALTSDGSRLILY 300

Query: 322 PCEHQSNEKWSFQRDGTIRYSNKCLTFDTSRF---VVLYNCSKVEAKSTKWNVAIDGTIS 381
           PC  Q N+KW+F  DGT+R   KCL  + S+F   VV+Y+CSK+ A+   W+V++ GTI 
Sbjct: 301 PCGQQVNQKWTFHSDGTVRSLGKCLATNNSKFGNLVVIYDCSKLAAEDISWDVSVGGTIM 360

Query: 382 NPS-SGLVLTANSSTSNTQLIVEANKYTIGQGWRVGNYVEPIIGSIIGLEQMCLEATNNN 441
           NP+   L LT+N +T +T L +E N Y+  QGWRVGNYV+PIIGSI+GL+ MCLEAT+ N
Sbjct: 361 NPNYEDLALTSNKATRSTNLTMEVNTYSASQGWRVGNYVQPIIGSIVGLDDMCLEATDGN 420

Query: 442 TNMWLENCVLNKAEQYWAVYSDGSIRVNSERNLCVTSSSDR--SSALIVIDECNGT 490
           TNMWLE CV N+ EQ WA+YSDG+IRV+  R LCVT+SS    +  +I I  C+G+
Sbjct: 421 TNMWLEECVPNQREQSWALYSDGTIRVDDNRELCVTASSSTYDNWKVITILNCDGS 467

BLAST of Bhi04G001653 vs. Swiss-Prot
Match: sp|Q41358|SNAIB_SAMNI (Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 2.5e-103
Identity = 206/523 (39.39%), Postives = 304/523 (58.13%), Query Frame = 0

Query: 35  DGYKNFINTIRERLTKNTSKLYDIPIL--KNSLPPTEPFITINLKNANNETIVLAIDVVN 94
           D Y+ F+  ++E++       +D+P+L  ++ +  +  F+ + L N + +T+ LAIDVVN
Sbjct: 45  DTYEPFLRALQEKVILGNHTAFDLPVLNPESQVSDSNRFVLVPLTNPSGDTVTLAIDVVN 104

Query: 95  LGVVGYRSNNTSYVFFNAPPEASEIVFPSTCRVLLRFDSDYESIEKASGISRLQTLLGTD 154
           L VV + SN  SY F  +     + +F  T +  L F  +Y S+E+  G  R+   LG  
Sbjct: 105 LYVVAFSSNGKSYFFSGSTAVQRDNLFVDTTQEELNFTGNYTSLERQVGFGRVYIPLGPK 164

Query: 155 PLNSAISNLFHY-----HQESLPQSFLVILQMVLEGVKFKFIEQSVIKSLKYGYNFKPSL 214
            L+ AIS+L  Y       + L +  LV++QMV E  +F++IE  +  S+     F P L
Sbjct: 165 SLDQAISSLRTYTLTAGDTKPLARGLLVVIQMVSEAARFRYIELRIRTSITDASEFTPDL 224

Query: 215 AMISLQDNWAKLSLQIQASTSLQGLFGEAINFYDSNNRIIQVDSIYYPIIITNIALQLYQ 274
            M+S+++NW+ +S +IQ +    G+F   +   D  N  I+V +      +T IA+ LY 
Sbjct: 225 LMLSMENNWSSMSSEIQQAQP-GGIFAGVVQLRDERNNSIEVTNFRRLFELTYIAVLLYG 284

Query: 275 C-----------NVSTNFIRMPSITS---DSPCYIQTQTSSISGREGFCIDATRGIQYDG 334
           C            +    I+MP       +  C +   T  ISG +G C+D   G   DG
Sbjct: 285 CAPVTSSSYSNNAIDAQIIKMPVFRGGEYEKVCSVVEVTRRISGWDGLCVDVRYGHYIDG 344

Query: 335 NPIISSPCEHQSNEKWSFQRDGTIRYSNKCLTFDTSRFVVLYNCSKVEAKSTKWNVAIDG 394
           NP+   PC ++ N+ W+F+ DGTIR+  KCLT  +S  V++Y+C+ V  ++TKW V+IDG
Sbjct: 345 NPVQLRPCGNECNQLWTFRTDGTIRWLGKCLTASSS--VMIYDCNTVPPEATKWVVSIDG 404

Query: 395 TISNPSSGLVLTANSSTSNTQLIVEANKYTIGQGWRVGNYVEPIIGSIIGLEQMCLEATN 454
           TI+NP SGLVLTA  +   T L +E N +   QGW VG+ VEP++  I+G +QMCL    
Sbjct: 405 TITNPHSGLVLTAPQAAEGTALSLENNIHAARQGWTVGD-VEPLVTFIVGYKQMCLRENG 464

Query: 455 NNTNMWLENCVLNKAEQYWAVYSDGSIRVNSERNLCVTSSSDRSSALIVIDECNGTANQR 514
            N  +WLE+CVLN+ +Q WA+Y DG+IRVNS R+LCVTS     S LIVI +C G+ NQR
Sbjct: 465 ENNFVWLEDCVLNRVQQEWALYGDGTIRVNSNRSLCVTSEDHEPSDLIVILKCEGSGNQR 524

Query: 515 WNFKADGTIFNLKTKLVVDVERAMVSLKRIILFRKTGNPNQDW 537
           W F  +GTI N   KL++DV +  VSL++IIL+R TGNPNQ W
Sbjct: 525 WVFNTNGTISNPNAKLLMDVAQRDVSLRKIILYRPTGNPNQQW 563

BLAST of Bhi04G001653 vs. Swiss-Prot
Match: sp|P33183|NIGB_SAMNI (Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2)

HSP 1 Score: 375.9 bits (964), Expect = 7.3e-103
Identity = 215/557 (38.60%), Postives = 325/557 (58.35%), Query Frame = 0

Query: 4   LTFYIIVFLSLATRSIDGNQELPLSSSDLLVDG-----YKNFINTIRERLTKNTSKLYDI 63
           L FYI+V   LA  S+ G Q +   S    +DG     Y++F++ +R+ +   T ++  +
Sbjct: 9   LYFYIVV---LAICSV-GIQGIDYPSVSFNLDGAKSATYRDFLSNLRKTVATGTYEVNGL 68

Query: 64  PILK--NSLPPTEPFITINLKNANNETIVLAIDVVNLGVVGYRSNNTSYVFFNAPPEASE 123
           P+L+  + +     F+ + L N N  T+ LA+DV NL VV +  N  SY F +A      
Sbjct: 69  PVLRRESEVQVKSRFVLVPLTNYNGNTVTLAVDVTNLYVVAFSGNANSYFFKDATEVQKS 128

Query: 124 IVFPSTCRVLLRFDSDYESIEKASGISRLQTLLGTDPLNSAISNLFHYHQESLPQSFLVI 183
            +F  T +  L F  +Y+++E A+   R    LG  PL+ AI++L  YH +S+ +S LV+
Sbjct: 129 NLFVGTKQNTLSFTGNYDNLETAANTRRESIELGPSPLDGAITSL--YHGDSVARSLLVV 188

Query: 184 LQMVLEGVKFKFIEQSVIKSLKYGYNFKPSLAMISLQDNWAKLSLQI-QASTSLQGLFG- 243
           +QMV E  +F++IEQ V +SL+   +F P+  M+S+++NW+ +SL+I QA  ++   FG 
Sbjct: 189 IQMVSEAARFRYIEQEVRRSLQQATSFTPNALMLSMENNWSSMSLEIQQAGNNVSPFFGT 248

Query: 244 -EAINFYDSNNRIIQVDSIYYPIIITNIALQLYQCN--VSTNFIRMP--------SITSD 303
            + +N YD  +R+  VD+      IT IA+ L++C+   + N IRMP             
Sbjct: 249 VQLLN-YDHTHRL--VDNFEELYKITGIAILLFRCSSPSNDNAIRMPLDLAGEDNKYNDG 308

Query: 304 SPCYIQTQ-TSSISGREGFCIDATRGIQYDGNPIISSPCEHQSNEKWSFQRDGTIRYSNK 363
             C ++T  T +I GR+G C+D   G   DG P+   PC  Q N++W+F  D TIR   K
Sbjct: 309 ETCTLRTSFTRNIVGRDGLCVDVRNGYDTDGTPLQLWPCGTQRNQRWTFDSDDTIRSMGK 368

Query: 364 CLT---FDTSRFVVLYNCSKVEAKSTKWNVAIDGTISNPSSGLVLTANSSTSNTQLIVEA 423
           C+T    +    +V++NCS     + KW V IDG+I NPSSGLV+TA  + S T L++E 
Sbjct: 369 CMTANGLNNGSNIVIFNCSTAAENAIKWEVPIDGSIINPSSGLVMTAPRAASRTILLLED 428

Query: 424 NKYTIGQGWRVGNYVEPIIGSIIGLEQMCLEATNNNTNMWLENCVLNKAEQYWAVYSDGS 483
           N Y   QGW V N V+PI+ SI+G ++MCL++   N  +W+E+C     +Q WA+Y D +
Sbjct: 429 NIYAASQGWTVTNNVKPIVASIVGYKEMCLQSNGENNGVWMEDCEATSLQQQWALYGDRT 488

Query: 484 IRVNSERNLCVTSSSDRSSALIVIDECNGTANQRWNFKADGTIFNLKTKLVVDVERAMVS 537
           IRVNS R LCVT++   S  LI+I +C G  +QRW F +DG I N K++ V+DV  + VS
Sbjct: 489 IRVNSTRGLCVTTNGYNSKDLIIILKCQGLPSQRWFFNSDGAIVNPKSRHVMDVRASNVS 548

BLAST of Bhi04G001653 vs. Swiss-Prot
Match: sp|O22415|SNAIF_SAMNI (Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 3.1e-101
Identity = 209/523 (39.96%), Postives = 302/523 (57.74%), Query Frame = 0

Query: 35  DGYKNFINTIRERLTKNTSKLYDIPIL--KNSLPPTEPFITINLKNANNETIVLAIDVVN 94
           D Y  F+  ++E++       +D+P+L  ++ +  +  F+ + L N + +T+ LAIDVVN
Sbjct: 45  DTYGPFLRALQEKVILGNHTAFDLPVLNPESQVSDSNRFVLVPLTNPSGDTVTLAIDVVN 104

Query: 95  LGVVGYRSNNTSYVFFNAPPEASEIVFPSTCRVLLRFDSDYESIEKASGISRLQTLLGTD 154
           L VV + SN  SY F  +     + +F  T +  L F  +Y S+E+  G  R+   LG  
Sbjct: 105 LYVVAFSSNGRSYFFSGSTAVQRDNLFVDTTQEELNFTGNYISLERQVGFGRVYIPLGPK 164

Query: 155 PLNSAISNLFHY-----HQESLPQSFLVILQMVLEGVKFKFIEQSVIKSLKYGYNFKPSL 214
            L  AIS+L  Y       + L +  LV++QMV E  +F++IE  +  S+     F P L
Sbjct: 165 SLAQAISSLRTYTLSAGDTKPLARGLLVVIQMVSEAARFRYIELRIRTSITDASEFTPDL 224

Query: 215 AMISLQDNWAKLSLQIQASTSLQGLFGEAINFYDSNNRIIQVDSIYYPIIITNIALQLYQ 274
            M+S+++NW+ +S +IQ +    G+F   +   D  N  I+V +      +T IA+ LY 
Sbjct: 225 LMLSMENNWSSMSSEIQQAQP-GGIFPGVVQLRDERNNPIEVTNFRRLFELTYIAVLLYG 284

Query: 275 C-----NVSTN------FIRMPSITS---DSPCYIQTQTSSISGREGFCIDATRGIQYDG 334
           C     N  TN       I+MP       +  C +   T  ISG +G C+D   G   DG
Sbjct: 285 CAPVTSNSYTNNAIDAQIIKMPVFRGGGYEKVCSVVEVTRRISGWDGLCVDVRDGHYIDG 344

Query: 335 NPIISSPCEHQSNEKWSFQRDGTIRYSNKCLTFDTSRFVVLYNCSKVEAKSTKWNVAIDG 394
           N +   PC ++ N+ W+F+ DGTIR+  KCLT  TS  V++Y+C+ V  ++TKW V+ DG
Sbjct: 345 NTVQLGPCGNECNQLWTFRTDGTIRWLGKCLT--TSSSVMIYDCNTVPPEATKWVVSTDG 404

Query: 395 TISNPSSGLVLTANSSTSNTQLIVEANKYTIGQGWRVGNYVEPIIGSIIGLEQMCLEATN 454
           TI+NP SGLVLTA  +   T L +E N +   QGW VG+ VEP++  I+G +QMCL    
Sbjct: 405 TITNPRSGLVLTAPQAAEGTALSLENNIHAARQGWTVGD-VEPLVTFIVGYKQMCLTENG 464

Query: 455 NNTNMWLENCVLNKAEQYWAVYSDGSIRVNSERNLCVTSSSDRSSALIVIDECNGTANQR 514
            N  +WLE+CVLN+ EQ WA+Y DG+IRVNS R+LCVTS     S LIVI +C G+ NQR
Sbjct: 465 ENNFVWLEDCVLNRVEQEWALYGDGTIRVNSNRSLCVTSEDHEPSDLIVILKCEGSGNQR 524

Query: 515 WNFKADGTIFNLKTKLVVDVERAMVSLKRIILFRKTGNPNQDW 537
           W F  +GTI N   KLV+DV ++ VSL++IIL+  TGNPNQ W
Sbjct: 525 WVFNTNGTISNPNAKLVMDVAQSNVSLRKIILYPPTGNPNQQW 563

BLAST of Bhi04G001653 vs. Swiss-Prot
Match: sp|Q9M653|RIPT_POLML (Ribosome-inactivating protein PMRIPt OS=Polygonatum multiflorum OX=45371 GN=RIPt PE=1 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 3.8e-91
Identity = 199/552 (36.05%), Postives = 308/552 (55.80%), Query Frame = 0

Query: 23  QELPLSSSDLLV-DGYKNFINTIRERLTKNT-SKLYDIPILKN---SLPPTEPFITINLK 82
           Q+ P    ++L  + Y +FI  +RE +T+   +K   IP+L N    +P  E F+ ++L 
Sbjct: 40  QDFPTIFFNILAGETYGDFIADLREIVTRTADTKNGSIPVLLNPAHPVPVRERFVKVHLT 99

Query: 83  NANNETIVLAIDVVNLGVVGYRSNNTSYVFFNAPPEASEIVFPSTCRVLLRFDSDYESIE 142
             N +T++LA+DV NL V  + +NN +Y F +      E +F     + L F S+Y S+E
Sbjct: 100 GRNGKTVILALDVTNLYVAAFSANNVAYFFRDFSALERENLFSGMLTIRLSFTSNYVSLE 159

Query: 143 KASGISRLQTLLGTDPLNSAISNLF----HYHQESLPQSFLVILQMVLEGVKFKFIEQSV 202
             +G+ R    LG  PL+ A +          + S+ ++ LV++QMV E  +F+ IE+ V
Sbjct: 160 HKAGVGRENISLGPTPLDEACTKSLWSGTTVTEASIAKALLVVIQMVSEAARFRHIEERV 219

Query: 203 IKSLKYG----YNFKPSLAMISLQDNWAKLSLQIQASTSLQGLFGEAINFYDSNNRIIQV 262
            +S          F+P   M+S+++ W  +SL++Q S    G+F   +   D + + ++V
Sbjct: 220 RRSFTAADHDQLTFRPDGLMLSMENEWPSMSLEVQRSIE-GGIFIGVVQLQDESFQPLRV 279

Query: 263 DSIYYPIIITNIALQLYQCN-------------VSTNFIRMP-------SITSDSPCYIQ 322
           D+       T +AL L++C               +   IRMP           +  C + 
Sbjct: 280 DNFNTLSRYTMVALLLFRCGHPRATAGTSSTTPAAAQIIRMPVDVLAGEEYYDEETCTVG 339

Query: 323 TQTSSISGREGFCIDATRGIQYDGNPIISSPCEHQSNEKWSFQRDGTIRYSNKCLT---F 382
             T  ISG +G C+D       DG PI   PC  Q N++W+F  DGTI+   KC+T   +
Sbjct: 340 EPTRRISGLDGLCMDVRNESNNDGIPIQLWPCGAQRNQQWTFHTDGTIQSMGKCMTSNGY 399

Query: 383 DTSRFVVLYNCSKVEA-KSTKWNVAIDGTISNPSSGLVLTANSSTSNTQLIVEANKYTIG 442
               +V+++NCS      +TKW V+IDG+I+NP SGLVLTA  +   T L+V  N ++  
Sbjct: 400 HPGDYVMIFNCSTAPVPDATKWVVSIDGSITNPHSGLVLTAPQAAQTTILLVVRNTHSAK 459

Query: 443 QGWRVGNYVEPIIGSIIGLEQMCLEATN-NNTNMWLENCVLNKAEQYWAVYSDGSIRVNS 502
           QG  VG+ VEPI+  I+G + MCL+  N NNT +WLE+C +++ +Q+WA+YSDG+IRV+S
Sbjct: 460 QGRSVGDDVEPIVTYIVGFKYMCLQGNNENNTRVWLEDCAVDRPQQWWALYSDGTIRVDS 519

Query: 503 ERNLCVTSSSDRSSALIVIDECNGTANQRWNFKADGTIFNLKTKLVVDVERAMVSLKRII 537
           +R+LCVTS    S   I+I  C+G  NQR  F  DGTI N   +LV+DV ++ V+L++II
Sbjct: 520 DRSLCVTSDGHSSRDAIIILTCDGGINQRLVFNTDGTILNPNAQLVMDVRQSNVALRQII 579

BLAST of Bhi04G001653 vs. TrEMBL
Match: tr|A0A1S3BDI7|A0A1S3BDI7_CUCME (rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103488711 PE=3 SV=1)

HSP 1 Score: 812.8 bits (2098), Expect = 4.7e-232
Identity = 404/541 (74.68%), Postives = 468/541 (86.51%), Query Frame = 0

Query: 1   MTSLTFYIIVFLSLATRSIDGNQELPLSSSDLLVDGYKNFINTIRERLTKNTSKLYDIPI 60
           MTSLTFYII FLSLATR IDGNQ   LSSSDLLVDGYKNFIN IR+RLT+NTSKLYDIPI
Sbjct: 1   MTSLTFYIIAFLSLATRRIDGNQH-SLSSSDLLVDGYKNFINVIRQRLTENTSKLYDIPI 60

Query: 61  LKNSLPPTEPFITINLKNANNETIVLAIDVVNLGVVGYRSNNTSYVFFNAPPEASEIVFP 120
           LK+SLPPT+ F+TIN+ N N+ETI LAID VNLGV+GYRSNNTSY+F +AP  A +IVFP
Sbjct: 61  LKSSLPPTQRFMTINIVNQNDETISLAIDKVNLGVLGYRSNNTSYIFLDAPLVAFDIVFP 120

Query: 121 STCRVLLRFDSDYESIEKASGISRLQTLLGTDPLNSAISNLFHYHQESLPQSFLVILQMV 180
            TCRVLL F+S+YESIEKASG +RLQTLLG +PLNSAISNLFHYH+ S+P+SFLVILQMV
Sbjct: 121 KTCRVLLGFNSNYESIEKASGTTRLQTLLGLEPLNSAISNLFHYHRASIPESFLVILQMV 180

Query: 181 LEGVKFKFIEQSVIKSLKYGYNFKPSLAMISLQDNWAKLSLQIQASTSLQGLFGEAINFY 240
           LEG KFKFIE+SVI+SLKYGYNFKP LA++SL+DNW KLS Q+QAS SLQGLFGEAI  Y
Sbjct: 181 LEGSKFKFIERSVIQSLKYGYNFKPGLAIVSLEDNWEKLSSQVQASPSLQGLFGEAIKLY 240

Query: 241 DSNNRIIQVDSIYYPIIITNIALQLYQCNVSTNFIRMPSITSDSPCYIQTQTSSISGREG 300
           DSNN++I+VDSIYY II TNIA QL+ CN+STNFIRMPS   DS C +QT+T++I+G+ G
Sbjct: 241 DSNNKVIEVDSIYYAIITTNIAFQLHHCNISTNFIRMPSDVGDS-CNVQTRTATITGQNG 300

Query: 301 FCIDATRGIQYDGNPIISSPCEHQSNEKWSFQRDGTIRYSNKCLTFDTSRFVVLYNCSKV 360
           FC+DA+ G+  D N II   C +QSN++W+F  D TIRYSNKCLTFDTSRFVVLYNCS+V
Sbjct: 301 FCVDASNGLHQDDNSIILYRCGYQSNQEWTFLSDKTIRYSNKCLTFDTSRFVVLYNCSEV 360

Query: 361 EAKST-KWNVAIDGTISNPSSGLVLTANSSTSNTQLIVEANKYTIGQGWRVGNYVEPIIG 420
           E KS  +WNVAIDGTISNPSSGLVLT N ST+++QLIVE NK+T  QGWRVGNYVEP+ G
Sbjct: 361 EKKSNIRWNVAIDGTISNPSSGLVLTTNPSTNSSQLIVEVNKFTTSQGWRVGNYVEPLTG 420

Query: 421 SIIGLEQMCLEATNNNTNMWLENCVLNKAEQYWAVYSDGSIRVNSERNLCVTSSSDRSSA 480
           SIIGLE+MCLEATNNNTN+WLE CV NKAEQYWAVY DGSIRVNS+RNLCV+SSS+R  A
Sbjct: 421 SIIGLEEMCLEATNNNTNVWLEKCVKNKAEQYWAVYGDGSIRVNSKRNLCVSSSSNRILA 480

Query: 481 LIVIDECNGTANQRWNFKADGTIFNLKTKLVVDVERAMVSLKRIILFRKTGNPNQDWGLF 540
           LI+I+ECNGT+NQRWNF A+G I N +TK+V+DV R MVS K+IIL+RKTG  NQ+W LF
Sbjct: 481 LIIIEECNGTSNQRWNFLANGAILNPETKMVMDVYRFMVSRKKIILYRKTGERNQEWALF 539

BLAST of Bhi04G001653 vs. TrEMBL
Match: tr|A0A0A0K979|A0A0A0K979_CUCSA (rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_6G040650 PE=3 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 7.3e-225
Identity = 399/542 (73.62%), Postives = 461/542 (85.06%), Query Frame = 0

Query: 1   MTSLTFYIIV-FLSLATRSIDGNQELPLSSSDLLVDGYKNFINTIRERLTKNTSKLYDIP 60
           MTSLTFYII+ FLSLAT SIDGNQ L LSSSDL+VDGYKNFIN IR+RLT+NT KLYDIP
Sbjct: 1   MTSLTFYIIIAFLSLATPSIDGNQ-LSLSSSDLVVDGYKNFINVIRQRLTENTLKLYDIP 60

Query: 61  ILKNSLPPTEPFITINLKNANNETIVLAIDVVNLGVVGYRSNNTSYVFFNAPPEASEIVF 120
           IL++SLPPT+ F+ IN+ N N+ETI LAID VNLGVVGYRSNN SY+F +AP  A ++VF
Sbjct: 61  ILESSLPPTQRFMNINIGNQNDETISLAIDRVNLGVVGYRSNNNSYIFLDAPKAALDVVF 120

Query: 121 PSTCRVLLRFDSDYESIEKASGISRLQTLLGTDPLNSAISNLFHYHQESLPQSFLVILQM 180
           P TCRV+L F+SDYESIEKASG +RLQTLLG +PLNSAISNLFHY +  +P SFLVILQM
Sbjct: 121 PETCRVVLGFNSDYESIEKASGTTRLQTLLGLEPLNSAISNLFHYRRADIPGSFLVILQM 180

Query: 181 VLEGVKFKFIEQSVIKSLKYGYNFKPSLAMISLQDNWAKLSLQIQASTSLQGLFGEAINF 240
           VLEG KFKFIEQSVI+SLKYGYNFKP LA++SL+DNW KLS QIQAS SLQGLFGEAI  
Sbjct: 181 VLEGSKFKFIEQSVIQSLKYGYNFKPGLAIVSLEDNWDKLSSQIQASPSLQGLFGEAIRL 240

Query: 241 YDSNNRIIQVDSIYYPIIITNIALQLYQCNVSTNFIRMPSITSDSPCYIQTQTSSISGRE 300
           YDSN++ I VDSIYY II TNIA QL+ CNVSTNFIRMPS   DS C +QT+T+ ISG+ 
Sbjct: 241 YDSNDKFIDVDSIYYVIITTNIAFQLHHCNVSTNFIRMPSDVDDS-CNVQTRTAMISGQN 300

Query: 301 GFCIDATRGIQYDGNPIISSPCEHQSNEKWSFQRDGTIRYSNKCLTFDTSRFVVLYNCSK 360
           GFC+D++R + Y+GNPII   C +Q N++W+F  D TIRYSNKCLTF+TSR+VVLYNCS+
Sbjct: 301 GFCVDSSRILDYNGNPIILYRCINQLNQEWTFLSDKTIRYSNKCLTFETSRYVVLYNCSE 360

Query: 361 VEAK-STKWNVAIDGTISNPSSGLVLTANSSTSNTQLIVEANKYTIGQGWRVGNYVEPII 420
           VE K + +WNVAIDGTISNPSSGLVLT + ST+ +QLIVE NK+T  QGWRVGNYV+PII
Sbjct: 361 VEEKGNIRWNVAIDGTISNPSSGLVLTTDPSTNRSQLIVEVNKFTTSQGWRVGNYVKPII 420

Query: 421 GSIIGLEQMCLEATNNNTNMWLENCVLNKAEQYWAVYSDGSIRVNSERNLCVTSSSDRSS 480
           GSIIG+E+MCLEATNNNTNMWLE CV NKAEQYWAVYSDGSIRVN +RNLCV+SSS+RS 
Sbjct: 421 GSIIGMEEMCLEATNNNTNMWLEKCVKNKAEQYWAVYSDGSIRVNRKRNLCVSSSSNRSG 480

Query: 481 ALIVIDECNGTANQRWNFKADGTIFNLKTKLVVDVERAMVSLKRIILFRKTGNPNQDWGL 540
           AL++IDEC GT+NQRWNF A+GTI N +TK VVDV  +MVS KRIIL+ KTG  NQ W L
Sbjct: 481 ALLIIDECKGTSNQRWNFLANGTILNPETKKVVDVYGSMVSAKRIILYPKTGLANQQWTL 540

BLAST of Bhi04G001653 vs. TrEMBL
Match: tr|A0A1S3C7Q1|A0A1S3C7Q1_CUCME (rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103497644 PE=3 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 7.7e-174
Identity = 310/539 (57.51%), Postives = 395/539 (73.28%), Query Frame = 0

Query: 6   FYIIVFLSLATRSIDG-NQELPLSSSDLLVDGYKNFINTIRERLTKNTSKLYDIPILKNS 65
           FYI+V LSLAT +I+G              + YK F+  +R  + +NTS+LY IPILK S
Sbjct: 2   FYIVVLLSLATSTIEGXXXXXXXXXXXXXXNNYKTFVQVLRHSIIENTSQLYGIPILKTS 61

Query: 66  LPPTEPFITINLKNANNETIVLAIDVVNLGVVGYRSNNTSYVFFNAPPEASEIVFPSTCR 125
            PP++ F T +L N  +ETI LA+D VNL VVGY SNNTSY+F +AP EA ++VFPSTCR
Sbjct: 62  FPPSQRFFTFSLANVIDETITLALDKVNLEVVGYLSNNTSYMFLDAPIEAFQVVFPSTCR 121

Query: 126 VLLRFDSDYESIEKASGISRLQTLLGTDPLNSAISNLFHYHQESLPQSFLVILQMVLEGV 185
           V++ F SDY+SIE A+  +R +TLLG DP++ AIS LFHY Q+S+P +FLVILQMV+EG+
Sbjct: 122 VIIDFYSDYKSIEIAANTTRFETLLGFDPIDVAISTLFHYRQDSVPNAFLVILQMVIEGM 181

Query: 186 KFKFIEQSVIKSLKYGYNFKPSLAMISLQDNWAKLSLQIQASTSLQGLFGEAINFYDSNN 245
           KFKFIEQSV  ++KYGYNFKPSLA +SLQDNW KLS QIQ S SLQG+FGE I  YDSN+
Sbjct: 182 KFKFIEQSVFMNIKYGYNFKPSLAFVSLQDNWVKLSSQIQVSPSLQGIFGEVIQLYDSND 241

Query: 246 RIIQVDSIYYPIIITNIALQL-YQCNVSTNFIRMPSITSDSPCYIQTQTSSISGREGFCI 305
           +IIQVD+I YPIII NIA+QL + CNVS   I+M     + PC +Q++T+ I GR G C+
Sbjct: 242 KIIQVDAIDYPIIIPNIAMQLHHHCNVSKKIIKM--TVQNDPCNVQSRTTHIIGRAGLCV 301

Query: 306 DATRGIQYDGNPIISSPCEHQSNEKWSFQRDGTIRYSNKCLTFDTSRFVVLYNCSKVEAK 365
           D    +  DG+P+I  PC  Q N++W+FQRDGTI+   KCLTF+ + FV++Y+CSKVE  
Sbjct: 302 DVENRLSNDGSPLILYPCRQQMNQRWTFQRDGTIQSLGKCLTFNDTNFVLIYDCSKVEPS 361

Query: 366 STKWNVAIDGTISNPSSGLVLTANSSTSNTQLIVEANKYTIGQGWRVGNYVEPIIGSIIG 425
             KW V++DGTISNPSSGLVLTANS    T+L +EAN+ TIGQ WRVGNY+ PI+GSIIG
Sbjct: 362 VIKWKVSVDGTISNPSSGLVLTANSPARTTKLKMEANQQTIGQCWRVGNYINPIVGSIIG 421

Query: 426 LEQMCLEATNNNTNMWLENCVLNKAEQYWAVYSDGSIRVNSERNLCV--TSSSDRSSALI 485
           L+ +CL ATNNNTN+ L++C  NK+EQYWA+Y DG+IRVNS RNLCV  TSS      +I
Sbjct: 422 LDGVCLVATNNNTNIELDDCAKNKSEQYWALYGDGTIRVNSSRNLCVSLTSSDCTPGRII 481

Query: 486 VIDECNGTANQRWNFKADGTIFNLKTKLVVDVERAMVSLKRIILFRKTGNPNQDWGLFY 541
            +  CNG+++QRWNF+ADG+I N K  + +DV+        IIL+ KTG   Q W LFY
Sbjct: 482 TVLMCNGSSSQRWNFQADGSIINPKCGMAIDVDTRWYD---IILYPKTGEYTQQWSLFY 535

BLAST of Bhi04G001653 vs. TrEMBL
Match: tr|A0A1S3CIM5|A0A1S3CIM5_CUCME (rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103501401 PE=3 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 1.7e-141
Identity = 267/541 (49.35%), Postives = 367/541 (67.84%), Query Frame = 0

Query: 9   IVFLSLATRSIDGNQELPLSSSDLLVDGYKNFINTIRERLTKNTSKLYDIPILKNSLPPT 68
           +V LS AT ++         S   +VD Y+++I TIR++    T KLY IP+L++SL  +
Sbjct: 8   VVTLSFATNAL---------SKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVLQHSLSNS 67

Query: 69  EPFITINLKNANNETIVLAIDVVNLGVVGY-RSNNTSYVFFNAPPEASEIVFPSTCRVLL 128
           + F  I+  N   +TI LA+D  ++ VV Y   N+ SY F NAP  A +I+FP+T + LL
Sbjct: 68  DRFHLIDTGNEPGDTITLAVDAQDMSVVAYLAGNDDSYFFSNAPKFAFDILFPNTNQNLL 127

Query: 129 RFDSDYESIEKASGISRLQTLLGTDPLNSAISNLFHYHQESLPQSFLVILQMVLEGVKFK 188
            FDS ++SIEKA+  +R  T LG    N+AI+NLFHY     P SFL++ QM+ E  KF 
Sbjct: 128 NFDSSFKSIEKAANTTRQATPLGFKESNAAIANLFHYDSVLAPVSFLIVFQMIFESAKFN 187

Query: 189 FIEQSVIKSLKYGYNFKPSLAMISLQDNWAKLSLQIQASTSLQGLFGEAINFYDSNNRII 248
           FIEQ ++ S+KYG  F P LAM+SL+DNW++LSLQIQAS SLQGLFG ++  Y+S N  I
Sbjct: 188 FIEQRIVNSIKYGEAFTPDLAMLSLEDNWSELSLQIQASNSLQGLFGSSVTLYNSKNEAI 247

Query: 249 QVDSIYYPIIITNIALQLYQCNVSTNFIRMPSIT----SDSPCYIQTQTSSISGREGFCI 308
           +VDSIYYP+I++N+ALQLY CN+  ++I+MP+ T     +  CY++ +T  ISGR+G C+
Sbjct: 248 EVDSIYYPLILSNLALQLYHCNIQ-DYIKMPTATVTDHQNPRCYVKERTVRISGRDGLCV 307

Query: 309 DATRGIQYDGNPIISSPCEHQSNEKWSFQRDGTIRYSNKCLTFDTSR---FVVLYNCSKV 368
           D  R    DG+ +ISSPC  Q+N++W+F RD TIR S+KCL    S+   F V+ +C+KV
Sbjct: 308 DVAR----DGSQVISSPCGQQANQRWTFNRDHTIRSSDKCLIPTKSKANPFAVILDCNKV 367

Query: 369 EAKSTKWNVAIDGTISNPSSGLVLTANSSTSNTQLIVEANKYTIGQGWRVGNYVEPIIGS 428
             +   W+V+I GTI NP+  LVLT+N+  ++  L ++ NKY   QGWRVGN+VEP+I S
Sbjct: 368 SQQDATWDVSISGTIMNPAYDLVLTSNNGINDNSLSMKKNKYCGNQGWRVGNFVEPLIVS 427

Query: 429 IIGLEQMCLEATNNNTNMWLENCVLNKAEQYWAVYSDGSIRVNSERNLCVTSSSDRSSAL 488
           IIG++QMCLEAT  NTN+WLE CV NK EQ WAVYSDGSIRVN++ +LCVT+SS      
Sbjct: 428 IIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVYSDGSIRVNNDHSLCVTASSIEPKQR 487

Query: 489 IVIDECNGTANQRWNFKADGTIFNLKTK-LVVDVERAMVSLKRIILFRKTGNPNQDWGLF 541
           IVI +CNG A+QRW   ADGTI   K + LV+DV ++ V LK I+L+ ++   +Q W   
Sbjct: 488 IVIGKCNGLASQRWVLNADGTISTPKNEGLVMDVAQSNVDLKEIVLYPRSDLVSQQWVAL 534

BLAST of Bhi04G001653 vs. TrEMBL
Match: tr|A0A0A0KN71|A0A0A0KN71_CUCSA (rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_5G428440 PE=3 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 3.1e-138
Identity = 264/541 (48.80%), Postives = 364/541 (67.28%), Query Frame = 0

Query: 9   IVFLSLATRSIDGNQELPLSSSDLLVDGYKNFINTIRERLTKNTSKLYDIPILKNSLPPT 68
           IV LS+AT ++         S   +VD Y+++I TIR++    T KLY IP+L +SL  +
Sbjct: 8   IVTLSIATNAL---------SKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVLHHSLSNS 67

Query: 69  EPFITINLKNANNETIVLAIDVVNLGVVGY-RSNNTSYVFFNAPPEASEIVFPSTCRVLL 128
           + F  I+  N   +TI  A+D  ++ VV Y   +N SY F NAP  A +I+FP T + LL
Sbjct: 68  DRFHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFPKTNQNLL 127

Query: 129 RFDSDYESIEKASGISRLQTLLGTDPLNSAISNLFHYHQESLPQSFLVILQMVLEGVKFK 188
            FD+ ++SIE A+  +R  T LG  P N+AI+NLFHY     P SFL++ QMV E  KFK
Sbjct: 128 NFDNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMVFESAKFK 187

Query: 189 FIEQSVIKSLKYGYNFKPSLAMISLQDNWAKLSLQIQASTSLQGLFGEAINFYDSNNRII 248
           FIEQ ++ S+  G  F P LAM+SL+DNW++LSL+IQAS SLQGLFG ++  Y+S N  +
Sbjct: 188 FIEQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLYNSKNEAV 247

Query: 249 QVDSIYYPIIITNIALQLYQCNVSTNFIRMPSIT----SDSPCYIQTQTSSISGREGFCI 308
           +VDSIYYP+I++N+ALQLY CN+  ++I+MP+ T     +  CY++ +T  ISG++G C 
Sbjct: 248 EVDSIYYPLILSNLALQLYHCNIQ-DYIKMPTATVADHQNPRCYVKERTVRISGQDGLCA 307

Query: 309 DATRGIQYDGNPIISSPCEHQSNEKWSFQRDGTIRYSNKCLTFDTSR---FVVLYNCSKV 368
           D  R    DG+ +ISSPC  Q+N++W+F RD TIR S+KCL  + S+     V+ NC+KV
Sbjct: 308 DVAR----DGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQNCNKV 367

Query: 369 EAKSTKWNVAIDGTISNPSSGLVLTANSSTSNTQLIVEANKYTIGQGWRVGNYVEPIIGS 428
             +   W+V+I GTI NP+  LVLT+ +  ++  L ++ NKY   QGWRVGNYVEPII S
Sbjct: 368 SQEDATWDVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVEPIIAS 427

Query: 429 IIGLEQMCLEATNNNTNMWLENCVLNKAEQYWAVYSDGSIRVNSERNLCVTSSSDRSSAL 488
           IIG++QMCLEAT  NTN+WLE CV NK EQ WAV+SDGSIRVN++ +LC+T+SS  S   
Sbjct: 428 IIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSIESKQR 487

Query: 489 IVIDECNGTANQRWNFKADGTIFNLKTK-LVVDVERAMVSLKRIILFRKTGNPNQDWGLF 541
           IVI +CNG A+QRW  KADGTI   K + LV+DV ++ V LK I+L+ ++   +Q W   
Sbjct: 488 IVIAKCNGLASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVSQHWVAL 534

BLAST of Bhi04G001653 vs. NCBI nr
Match: XP_008445788.1 (PREDICTED: nigrin b-like [Cucumis melo])

HSP 1 Score: 812.8 bits (2098), Expect = 7.2e-232
Identity = 404/541 (74.68%), Postives = 468/541 (86.51%), Query Frame = 0

Query: 1   MTSLTFYIIVFLSLATRSIDGNQELPLSSSDLLVDGYKNFINTIRERLTKNTSKLYDIPI 60
           MTSLTFYII FLSLATR IDGNQ   LSSSDLLVDGYKNFIN IR+RLT+NTSKLYDIPI
Sbjct: 1   MTSLTFYIIAFLSLATRRIDGNQH-SLSSSDLLVDGYKNFINVIRQRLTENTSKLYDIPI 60

Query: 61  LKNSLPPTEPFITINLKNANNETIVLAIDVVNLGVVGYRSNNTSYVFFNAPPEASEIVFP 120
           LK+SLPPT+ F+TIN+ N N+ETI LAID VNLGV+GYRSNNTSY+F +AP  A +IVFP
Sbjct: 61  LKSSLPPTQRFMTINIVNQNDETISLAIDKVNLGVLGYRSNNTSYIFLDAPLVAFDIVFP 120

Query: 121 STCRVLLRFDSDYESIEKASGISRLQTLLGTDPLNSAISNLFHYHQESLPQSFLVILQMV 180
            TCRVLL F+S+YESIEKASG +RLQTLLG +PLNSAISNLFHYH+ S+P+SFLVILQMV
Sbjct: 121 KTCRVLLGFNSNYESIEKASGTTRLQTLLGLEPLNSAISNLFHYHRASIPESFLVILQMV 180

Query: 181 LEGVKFKFIEQSVIKSLKYGYNFKPSLAMISLQDNWAKLSLQIQASTSLQGLFGEAINFY 240
           LEG KFKFIE+SVI+SLKYGYNFKP LA++SL+DNW KLS Q+QAS SLQGLFGEAI  Y
Sbjct: 181 LEGSKFKFIERSVIQSLKYGYNFKPGLAIVSLEDNWEKLSSQVQASPSLQGLFGEAIKLY 240

Query: 241 DSNNRIIQVDSIYYPIIITNIALQLYQCNVSTNFIRMPSITSDSPCYIQTQTSSISGREG 300
           DSNN++I+VDSIYY II TNIA QL+ CN+STNFIRMPS   DS C +QT+T++I+G+ G
Sbjct: 241 DSNNKVIEVDSIYYAIITTNIAFQLHHCNISTNFIRMPSDVGDS-CNVQTRTATITGQNG 300

Query: 301 FCIDATRGIQYDGNPIISSPCEHQSNEKWSFQRDGTIRYSNKCLTFDTSRFVVLYNCSKV 360
           FC+DA+ G+  D N II   C +QSN++W+F  D TIRYSNKCLTFDTSRFVVLYNCS+V
Sbjct: 301 FCVDASNGLHQDDNSIILYRCGYQSNQEWTFLSDKTIRYSNKCLTFDTSRFVVLYNCSEV 360

Query: 361 EAKST-KWNVAIDGTISNPSSGLVLTANSSTSNTQLIVEANKYTIGQGWRVGNYVEPIIG 420
           E KS  +WNVAIDGTISNPSSGLVLT N ST+++QLIVE NK+T  QGWRVGNYVEP+ G
Sbjct: 361 EKKSNIRWNVAIDGTISNPSSGLVLTTNPSTNSSQLIVEVNKFTTSQGWRVGNYVEPLTG 420

Query: 421 SIIGLEQMCLEATNNNTNMWLENCVLNKAEQYWAVYSDGSIRVNSERNLCVTSSSDRSSA 480
           SIIGLE+MCLEATNNNTN+WLE CV NKAEQYWAVY DGSIRVNS+RNLCV+SSS+R  A
Sbjct: 421 SIIGLEEMCLEATNNNTNVWLEKCVKNKAEQYWAVYGDGSIRVNSKRNLCVSSSSNRILA 480

Query: 481 LIVIDECNGTANQRWNFKADGTIFNLKTKLVVDVERAMVSLKRIILFRKTGNPNQDWGLF 540
           LI+I+ECNGT+NQRWNF A+G I N +TK+V+DV R MVS K+IIL+RKTG  NQ+W LF
Sbjct: 481 LIIIEECNGTSNQRWNFLANGAILNPETKMVMDVYRFMVSRKKIILYRKTGERNQEWALF 539

BLAST of Bhi04G001653 vs. NCBI nr
Match: XP_011656527.1 (PREDICTED: nigrin b-like [Cucumis sativus] >KGN45978.1 hypothetical protein Csa_6G040650 [Cucumis sativus])

HSP 1 Score: 788.9 bits (2036), Expect = 1.1e-224
Identity = 399/542 (73.62%), Postives = 461/542 (85.06%), Query Frame = 0

Query: 1   MTSLTFYIIV-FLSLATRSIDGNQELPLSSSDLLVDGYKNFINTIRERLTKNTSKLYDIP 60
           MTSLTFYII+ FLSLAT SIDGNQ L LSSSDL+VDGYKNFIN IR+RLT+NT KLYDIP
Sbjct: 1   MTSLTFYIIIAFLSLATPSIDGNQ-LSLSSSDLVVDGYKNFINVIRQRLTENTLKLYDIP 60

Query: 61  ILKNSLPPTEPFITINLKNANNETIVLAIDVVNLGVVGYRSNNTSYVFFNAPPEASEIVF 120
           IL++SLPPT+ F+ IN+ N N+ETI LAID VNLGVVGYRSNN SY+F +AP  A ++VF
Sbjct: 61  ILESSLPPTQRFMNINIGNQNDETISLAIDRVNLGVVGYRSNNNSYIFLDAPKAALDVVF 120

Query: 121 PSTCRVLLRFDSDYESIEKASGISRLQTLLGTDPLNSAISNLFHYHQESLPQSFLVILQM 180
           P TCRV+L F+SDYESIEKASG +RLQTLLG +PLNSAISNLFHY +  +P SFLVILQM
Sbjct: 121 PETCRVVLGFNSDYESIEKASGTTRLQTLLGLEPLNSAISNLFHYRRADIPGSFLVILQM 180

Query: 181 VLEGVKFKFIEQSVIKSLKYGYNFKPSLAMISLQDNWAKLSLQIQASTSLQGLFGEAINF 240
           VLEG KFKFIEQSVI+SLKYGYNFKP LA++SL+DNW KLS QIQAS SLQGLFGEAI  
Sbjct: 181 VLEGSKFKFIEQSVIQSLKYGYNFKPGLAIVSLEDNWDKLSSQIQASPSLQGLFGEAIRL 240

Query: 241 YDSNNRIIQVDSIYYPIIITNIALQLYQCNVSTNFIRMPSITSDSPCYIQTQTSSISGRE 300
           YDSN++ I VDSIYY II TNIA QL+ CNVSTNFIRMPS   DS C +QT+T+ ISG+ 
Sbjct: 241 YDSNDKFIDVDSIYYVIITTNIAFQLHHCNVSTNFIRMPSDVDDS-CNVQTRTAMISGQN 300

Query: 301 GFCIDATRGIQYDGNPIISSPCEHQSNEKWSFQRDGTIRYSNKCLTFDTSRFVVLYNCSK 360
           GFC+D++R + Y+GNPII   C +Q N++W+F  D TIRYSNKCLTF+TSR+VVLYNCS+
Sbjct: 301 GFCVDSSRILDYNGNPIILYRCINQLNQEWTFLSDKTIRYSNKCLTFETSRYVVLYNCSE 360

Query: 361 VEAK-STKWNVAIDGTISNPSSGLVLTANSSTSNTQLIVEANKYTIGQGWRVGNYVEPII 420
           VE K + +WNVAIDGTISNPSSGLVLT + ST+ +QLIVE NK+T  QGWRVGNYV+PII
Sbjct: 361 VEEKGNIRWNVAIDGTISNPSSGLVLTTDPSTNRSQLIVEVNKFTTSQGWRVGNYVKPII 420

Query: 421 GSIIGLEQMCLEATNNNTNMWLENCVLNKAEQYWAVYSDGSIRVNSERNLCVTSSSDRSS 480
           GSIIG+E+MCLEATNNNTNMWLE CV NKAEQYWAVYSDGSIRVN +RNLCV+SSS+RS 
Sbjct: 421 GSIIGMEEMCLEATNNNTNMWLEKCVKNKAEQYWAVYSDGSIRVNRKRNLCVSSSSNRSG 480

Query: 481 ALIVIDECNGTANQRWNFKADGTIFNLKTKLVVDVERAMVSLKRIILFRKTGNPNQDWGL 540
           AL++IDEC GT+NQRWNF A+GTI N +TK VVDV  +MVS KRIIL+ KTG  NQ W L
Sbjct: 481 ALLIIDECKGTSNQRWNFLANGTILNPETKKVVDVYGSMVSAKRIILYPKTGLANQQWTL 540

BLAST of Bhi04G001653 vs. NCBI nr
Match: XP_023547577.1 (seed lectin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 665.6 bits (1716), Expect = 1.4e-187
Identity = 336/540 (62.22%), Postives = 410/540 (75.93%), Query Frame = 0

Query: 1   MTSLTFYIIVFLSLATRSIDGNQELPLSSSDLLVDGYKNFINTIRERLTKNTSKLYDIPI 60
           M SL  YI+ FL +AT +I+GN  L  SS+D   D YKNFI  +R+  T NTS LYDIPI
Sbjct: 1   MRSLALYILAFLCVATPNIEGN-HLCFSSTDSDADSYKNFIQQLRQYFTINTSILYDIPI 60

Query: 61  LKNSLPPTEPFITINLKNANNETIVLAIDVVNLGVVGYRSNNTSYVFFNAPPEASEIVFP 120
           LK+S+PP++ F+T+NL N   E I +A+DV+ L  VGYRSN+TSYVFF+AP  A EIVFP
Sbjct: 61  LKHSVPPSQRFLTLNLDNPYGEDIFVAVDVLTLRPVGYRSNDTSYVFFDAPRVAFEIVFP 120

Query: 121 STCRVLLRFDSDYESIEKASGISRLQTLLGTDPLNSAISNLFHYHQESLPQSFLVILQMV 180
           STCRVLL F+SD+ESIE A+G SRL TLLG  P  SA+S+LFHY ++S+P SFLVI+QM+
Sbjct: 121 STCRVLLGFNSDFESIENAAGTSRLDTLLGLAPFESAVSDLFHYRRDSVPTSFLVIIQML 180

Query: 181 LEGVKFKFIEQSVIKSLKYGYNFKPSLAMISLQDNWAKLSLQIQASTSLQGLFGEAINFY 240
           LE  KFKFIEQSV+ SLK G  FKP+LA++SLQDNWAKLSLQIQAS+SLQGLFGE I  Y
Sbjct: 181 LEATKFKFIEQSVVNSLKNGVPFKPTLAILSLQDNWAKLSLQIQASSSLQGLFGEPIELY 240

Query: 241 DSNNRIIQVDSIYYPIIITNIALQLYQCNVSTNFIRMPSITSDSPCYIQTQTSSISGREG 300
           DSN RIIQVDSIYYPIII ++ALQLYQCN+ TNFIRMPS+  D PCY+QT+T+ ISG EG
Sbjct: 241 DSNQRIIQVDSIYYPIIIASMALQLYQCNIGTNFIRMPSVGGD-PCYVQTRTARISGLEG 300

Query: 301 FCIDATRGIQYDGNPIISSPCEHQSNEKWSFQRDGTIRYSNKCLTFDTSRFVVLYNCSKV 360
           FCI+A      DGNP+I S C  Q  ++WSFQ DG I YS KCL+F+ S +VV YNCS+ 
Sbjct: 301 FCIEAENKGWSDGNPVILSQCGGQLTQRWSFQSDGRIGYSGKCLSFNLSSYVVTYNCSEA 360

Query: 361 EAKSTKWNVAIDGTISNPSSGLVLTANSSTSNTQLIVEANKYTIGQGWRVGNYVEPIIGS 420
            A S +W V+++G ISNP+S LVLTAN+S  +  L  E N  T+GQ WRVGNYV PI+GS
Sbjct: 361 LAGSIRWKVSVEGMISNPNSDLVLTANASRRSAMLTAEPNAKTLGQSWRVGNYVWPIVGS 420

Query: 421 IIGLEQMCLEATNNNTNMWLENCVLNKAEQYWAVYSDGSIRVNSERNLCVTSSSDRSSAL 480
           IIGLE+ CLE+ +NN  +WLE CV  KAEQYWAVYSDGSIRVNS RN CV+++SD    L
Sbjct: 421 IIGLEETCLESMDNNAKVWLEKCVEQKAEQYWAVYSDGSIRVNSSRNFCVSAASDSPGGL 480

Query: 481 IVIDECNGTANQRWNFKADGTIFNLKTKLVVDVERAMVSLKRIILFRKTGNPNQDWGLFY 540
           I I +CNG++ QRW F AD TI N K+ LV++VE + VS  +IIL+   G  +Q W LFY
Sbjct: 481 ITITKCNGSSKQRWVFMADATILNPKSGLVMEVEASQVSRNKIILYPIKGRSSQRWTLFY 538

BLAST of Bhi04G001653 vs. NCBI nr
Match: XP_022991446.1 (LOW QUALITY PROTEIN: seed lectin-like [Cucurbita maxima])

HSP 1 Score: 640.6 bits (1651), Expect = 4.9e-180
Identity = 327/540 (60.56%), Postives = 398/540 (73.70%), Query Frame = 0

Query: 1   MTSLTFYIIVFLSLATRSIDGNQELPLSSSDLLVDGYKNFINTIRERLTKNTSKLYDIPI 60
           M SL  YI+ FL +AT +I+GN  L  SSSD  V  YKNFI  +R+  T NTS LYDIPI
Sbjct: 1   MRSLALYILDFLCVATPNIEGN-HLCFSSSDSDVHSYKNFIQQLRQYFTINTSILYDIPI 60

Query: 61  LKNSLPPTEPFITINLKNANNETIVLAIDVVNLGVVGYRSNNTSYVFFNAPPEASEIVFP 120
           LK+S+PP++ F+TINL N   E I +A+DV+ L  VGYRSN+TSYVFF+AP  A E VFP
Sbjct: 61  LKHSVPPSQRFLTINLDNPYGEDIFVAVDVLTLRPVGYRSNDTSYVFFDAPRVAFETVFP 120

Query: 121 STCRVLLRFDSDYESIEKASGISRLQTLLGTDPLNSAISNLFHYHQESLPQSFLVILQMV 180
           STCRVLL F+SD+ESIE A+  SRL TLLG  P  SA+S+LFHY Q+      L   +  
Sbjct: 121 STCRVLLGFNSDFESIENAAATSRLDTLLGLAPFESAVSDLFHYRQDLSLSRSLSSFKFF 180

Query: 181 LEGVKFKFIEQSVIKSLKYGYNFKPSLAMISLQDNWAKLSLQIQASTSLQGLFGEAINFY 240
           LE  KFKFIEQSV+ SLK G  FKP+LA++SLQDNWAKLS QIQAS+SLQGLF E I  Y
Sbjct: 181 LEATKFKFIEQSVVNSLKNGVPFKPTLAILSLQDNWAKLSXQIQASSSLQGLFREPIELY 240

Query: 241 DSNNRIIQVDSIYYPIIITNIALQLYQCNVSTNFIRMPSITSDSPCYIQTQTSSISGREG 300
           DSN+RIIQVDSIYYPIII N+ALQLYQCN+ TNFIRMPS+  D PCY+QT+T+ ISG EG
Sbjct: 241 DSNHRIIQVDSIYYPIIIPNMALQLYQCNIGTNFIRMPSVGGD-PCYVQTRTARISGLEG 300

Query: 301 FCIDATRGIQYDGNPIISSPCEHQSNEKWSFQRDGTIRYSNKCLTFDTSRFVVLYNCSKV 360
           FC++A      DGNP+I SPC  Q  ++WSFQ DG I YS KCL+F+ S +VV YNCS+ 
Sbjct: 301 FCVEADNKGWSDGNPVILSPCGEQLTQRWSFQSDGRIGYSGKCLSFNLSSYVVTYNCSEG 360

Query: 361 EAKSTKWNVAIDGTISNPSSGLVLTANSSTSNTQLIVEANKYTIGQGWRVGNYVEPIIGS 420
            A S +W V+++G ISNP+S LVLTAN+S  +  L  E N  T+GQ WRVGNYV PI+GS
Sbjct: 361 AAGSIRWKVSVEGMISNPNSDLVLTANASRRSAMLTAEPNAKTLGQSWRVGNYVWPIVGS 420

Query: 421 IIGLEQMCLEATNNNTNMWLENCVLNKAEQYWAVYSDGSIRVNSERNLCVTSSSDRSSAL 480
           IIGLE+ CLE+ +NN  +WLE CV  KAEQYWA+YSDGSIRVNS RN CV+++SD    L
Sbjct: 421 IIGLEETCLESMDNNAKVWLEKCVEQKAEQYWALYSDGSIRVNSSRNFCVSAASDSPGGL 480

Query: 481 IVIDECNGTANQRWNFKADGTIFNLKTKLVVDVERAMVSLKRIILFRKTGNPNQDWGLFY 540
           I I +CNG++ QRW F AD TI N K+ LV++VE + VS  +IIL+   G  +Q W LFY
Sbjct: 481 ITITKCNGSSKQRWAFMADATILNPKSGLVMEVEGSQVSRSKIILYPIKGRSSQQWTLFY 538

BLAST of Bhi04G001653 vs. NCBI nr
Match: XP_008458115.1 (PREDICTED: nigrin b-like [Cucumis melo])

HSP 1 Score: 619.4 bits (1596), Expect = 1.2e-173
Identity = 310/539 (57.51%), Postives = 395/539 (73.28%), Query Frame = 0

Query: 6   FYIIVFLSLATRSIDG-NQELPLSSSDLLVDGYKNFINTIRERLTKNTSKLYDIPILKNS 65
           FYI+V LSLAT +I+G              + YK F+  +R  + +NTS+LY IPILK S
Sbjct: 2   FYIVVLLSLATSTIEGXXXXXXXXXXXXXXNNYKTFVQVLRHSIIENTSQLYGIPILKTS 61

Query: 66  LPPTEPFITINLKNANNETIVLAIDVVNLGVVGYRSNNTSYVFFNAPPEASEIVFPSTCR 125
            PP++ F T +L N  +ETI LA+D VNL VVGY SNNTSY+F +AP EA ++VFPSTCR
Sbjct: 62  FPPSQRFFTFSLANVIDETITLALDKVNLEVVGYLSNNTSYMFLDAPIEAFQVVFPSTCR 121

Query: 126 VLLRFDSDYESIEKASGISRLQTLLGTDPLNSAISNLFHYHQESLPQSFLVILQMVLEGV 185
           V++ F SDY+SIE A+  +R +TLLG DP++ AIS LFHY Q+S+P +FLVILQMV+EG+
Sbjct: 122 VIIDFYSDYKSIEIAANTTRFETLLGFDPIDVAISTLFHYRQDSVPNAFLVILQMVIEGM 181

Query: 186 KFKFIEQSVIKSLKYGYNFKPSLAMISLQDNWAKLSLQIQASTSLQGLFGEAINFYDSNN 245
           KFKFIEQSV  ++KYGYNFKPSLA +SLQDNW KLS QIQ S SLQG+FGE I  YDSN+
Sbjct: 182 KFKFIEQSVFMNIKYGYNFKPSLAFVSLQDNWVKLSSQIQVSPSLQGIFGEVIQLYDSND 241

Query: 246 RIIQVDSIYYPIIITNIALQL-YQCNVSTNFIRMPSITSDSPCYIQTQTSSISGREGFCI 305
           +IIQVD+I YPIII NIA+QL + CNVS   I+M     + PC +Q++T+ I GR G C+
Sbjct: 242 KIIQVDAIDYPIIIPNIAMQLHHHCNVSKKIIKM--TVQNDPCNVQSRTTHIIGRAGLCV 301

Query: 306 DATRGIQYDGNPIISSPCEHQSNEKWSFQRDGTIRYSNKCLTFDTSRFVVLYNCSKVEAK 365
           D    +  DG+P+I  PC  Q N++W+FQRDGTI+   KCLTF+ + FV++Y+CSKVE  
Sbjct: 302 DVENRLSNDGSPLILYPCRQQMNQRWTFQRDGTIQSLGKCLTFNDTNFVLIYDCSKVEPS 361

Query: 366 STKWNVAIDGTISNPSSGLVLTANSSTSNTQLIVEANKYTIGQGWRVGNYVEPIIGSIIG 425
             KW V++DGTISNPSSGLVLTANS    T+L +EAN+ TIGQ WRVGNY+ PI+GSIIG
Sbjct: 362 VIKWKVSVDGTISNPSSGLVLTANSPARTTKLKMEANQQTIGQCWRVGNYINPIVGSIIG 421

Query: 426 LEQMCLEATNNNTNMWLENCVLNKAEQYWAVYSDGSIRVNSERNLCV--TSSSDRSSALI 485
           L+ +CL ATNNNTN+ L++C  NK+EQYWA+Y DG+IRVNS RNLCV  TSS      +I
Sbjct: 422 LDGVCLVATNNNTNIELDDCAKNKSEQYWALYGDGTIRVNSSRNLCVSLTSSDCTPGRII 481

Query: 486 VIDECNGTANQRWNFKADGTIFNLKTKLVVDVERAMVSLKRIILFRKTGNPNQDWGLFY 541
            +  CNG+++QRWNF+ADG+I N K  + +DV+        IIL+ KTG   Q W LFY
Sbjct: 482 TVLMCNGSSSQRWNFQADGSIINPKCGMAIDVDTRWYD---IILYPKTGEYTQQWSLFY 535