Bhi04G001565 (gene) Wax gourd

NameBhi04G001565
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionVacuolar protein sorting-associated protein 28 homolog
Locationchr4 : 50400142 .. 50400771 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGTCAAGCTTTGGAACGACAAGCGCGAGAGAGAAATGTACGACAATTTTGTAGAGCTCTACGCCATTATCAAGGCCACGGAAAAGCTCGAAAAGGCTTACATCCGTGACATTATCCCTTCCTCCGATTACGAGACCGAATGCTTCAAACTCATCGCACATTTTAAGACACTGGCCTCGACGCTTAAGGACACAGTCCCAAGCATTGAACTATTTGCAGATACTTACAAAATGGACTGTCCAGCGGCGATTAACCGTCTTGTGATAACTGGGGTGCCTGCCACGGTGGAGCATAGGGCGGCCGCTGCTGCCTCCGCCACTACCTCCGCTGCTATTGTGGCGGAGTGTGTGCAGAACTTCATTACGGCTATGGATTCGTTGAAATTGAACATGGTGGCGGTTGATCAGGTGCACCCCTTGCTCTCGGATCTCTCTGCTTCATTGAACAAATTGACCATTCTGCCGCCGGATTTTGAAGGGAAAACAAAGATGAAGGAGTGGCTTGGACGACTTTCGAAAATGGATGCGGCGGATGAGTTAACAGAGCAGCAAGCTCGGCAGCTTCACTTTGATCTTGAATCTTCTTATAATTCATTCATGGCGACTTTGCCTACAGCTGGTACTTGA

mRNA sequence

ATGGAGGTCAAGCTTTGGAACGACAAGCGCGAGAGAGAAATGTACGACAATTTTGTAGAGCTCTACGCCATTATCAAGGCCACGGAAAAGCTCGAAAAGGCTTACATCCGTGACATTATCCCTTCCTCCGATTACGAGACCGAATGCTTCAAACTCATCGCACATTTTAAGACACTGGCCTCGACGCTTAAGGACACAGTCCCAAGCATTGAACTATTTGCAGATACTTACAAAATGGACTGTCCAGCGGCGATTAACCGTCTTGTGATAACTGGGGTGCCTGCCACGGTGGAGCATAGGGCGGCCGCTGCTGCCTCCGCCACTACCTCCGCTGCTATTGTGGCGGAGTGTGTGCAGAACTTCATTACGGCTATGGATTCGTTGAAATTGAACATGGTGGCGGTTGATCAGGTGCACCCCTTGCTCTCGGATCTCTCTGCTTCATTGAACAAATTGACCATTCTGCCGCCGGATTTTGAAGGGAAAACAAAGATGAAGGAGTGGCTTGGACGACTTTCGAAAATGGATGCGGCGGATGAGTTAACAGAGCAGCAAGCTCGGCAGCTTCACTTTGATCTTGAATCTTCTTATAATTCATTCATGGCGACTTTGCCTACAGCTGGTACTTGA

Coding sequence (CDS)

ATGGAGGTCAAGCTTTGGAACGACAAGCGCGAGAGAGAAATGTACGACAATTTTGTAGAGCTCTACGCCATTATCAAGGCCACGGAAAAGCTCGAAAAGGCTTACATCCGTGACATTATCCCTTCCTCCGATTACGAGACCGAATGCTTCAAACTCATCGCACATTTTAAGACACTGGCCTCGACGCTTAAGGACACAGTCCCAAGCATTGAACTATTTGCAGATACTTACAAAATGGACTGTCCAGCGGCGATTAACCGTCTTGTGATAACTGGGGTGCCTGCCACGGTGGAGCATAGGGCGGCCGCTGCTGCCTCCGCCACTACCTCCGCTGCTATTGTGGCGGAGTGTGTGCAGAACTTCATTACGGCTATGGATTCGTTGAAATTGAACATGGTGGCGGTTGATCAGGTGCACCCCTTGCTCTCGGATCTCTCTGCTTCATTGAACAAATTGACCATTCTGCCGCCGGATTTTGAAGGGAAAACAAAGATGAAGGAGTGGCTTGGACGACTTTCGAAAATGGATGCGGCGGATGAGTTAACAGAGCAGCAAGCTCGGCAGCTTCACTTTGATCTTGAATCTTCTTATAATTCATTCATGGCGACTTTGCCTACAGCTGGTACTTGA

Protein sequence

MEVKLWNDKREREMYDNFVELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLASTLKDTVPSIELFADTYKMDCPAAINRLVITGVPATVEHRAAAAASATTSAAIVAECVQNFITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMDAADELTEQQARQLHFDLESSYNSFMATLPTAGT
BLAST of Bhi04G001565 vs. Swiss-Prot
Match: sp|Q9S9T7|VP282_ARATH (Vacuolar protein sorting-associated protein 28 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=VPS28-2 PE=1 SV=2)

HSP 1 Score: 337.0 bits (863), Expect = 1.5e-91
Identity = 170/208 (81.73%), Postives = 185/208 (88.94%), Query Frame = 0

Query: 1   MEVKLWNDKREREMYDNFVELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMY+NF EL+AIIKATEKLEKAYIRD+I  S+YE+EC KLI HFKTL+
Sbjct: 2   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDLINPSEYESECQKLIVHFKTLS 61

Query: 61  STLKDTVPSIELFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN 120
           +TLKDTVP+IE FADTYKMDCPAA+ RLV +G+PATVEHRA           IVAECVQN
Sbjct: 62  ATLKDTVPNIERFADTYKMDCPAALYRLVTSGLPATVEHRATVAASTSNSASIVAECVQN 121

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMDAADE 180
           FIT+MDSLKLNMVAVDQV+PLLSDLSASLNKL+ILPPDFEGKTKMKEWL RLSKM AADE
Sbjct: 122 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFEGKTKMKEWLSRLSKMGAADE 181

Query: 181 LTEQQARQLHFDLESSYNSFMATLPTAG 209
           LTEQQ+RQLHFDLESSYNSFMA LP AG
Sbjct: 182 LTEQQSRQLHFDLESSYNSFMAALPKAG 209

BLAST of Bhi04G001565 vs. Swiss-Prot
Match: sp|O65421|VP281_ARATH (Vacuolar protein sorting-associated protein 28 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VPS28-1 PE=1 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 2.3e-89
Identity = 167/208 (80.29%), Postives = 182/208 (87.50%), Query Frame = 0

Query: 1   MEVKLWNDKREREMYDNFVELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMY+NF ELYAIIKATEKLEKAYIRD+I  S+YETEC KLI HFKTL+
Sbjct: 1   MEVKLWNDKREREMYENFAELYAIIKATEKLEKAYIRDLISPSEYETECQKLIVHFKTLS 60

Query: 61  STLKDTVPSIELFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN 120
           ++LKD VP+IE FA+TYKMDC AA+ RLV +GVPATVEHRA           +VAECVQN
Sbjct: 61  ASLKDMVPNIERFAETYKMDCSAAVYRLVTSGVPATVEHRAAASASTSSSASVVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMDAADE 180
           FIT+MDSLKLNMVAVDQV+PLLSDLSASLNKL+ILPPDFEGK KMKEWL RLSKM A+DE
Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFEGKIKMKEWLLRLSKMGASDE 180

Query: 181 LTEQQARQLHFDLESSYNSFMATLPTAG 209
           LTEQQARQLHFDLESSYNSFMA LP AG
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPNAG 208

BLAST of Bhi04G001565 vs. Swiss-Prot
Match: sp|Q54NF1|VPS28_DICDI (Vacuolar protein sorting-associated protein 28 OS=Dictyostelium discoideum OX=44689 GN=vps28 PE=3 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.2e-50
Identity = 106/199 (53.27%), Postives = 134/199 (67.34%), Query Frame = 0

Query: 2   EVKLWNDKREREMYDNFVELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLAS 61
           EVKL+N+  EREMY+N  ELY+IIK TE LEKAYIRD +   DY T C KLIA FK+  +
Sbjct: 84  EVKLFNNNIEREMYENLAELYSIIKVTEHLEKAYIRDDVSPKDYTTACSKLIAQFKSSQT 143

Query: 62  TLKDTVPSIELFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQNF 121
            LKD V ++  F   Y ++C AA +RLVI G P+T+EH              VAE VQ F
Sbjct: 144 LLKDQVSNVGQFMKDYDLNCKAAFDRLVIKGFPSTLEHNTNESSTDSAMAKNVAEAVQLF 203

Query: 122 ITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMDAADEL 181
           IT MDS++L +V+VD ++PLLSDL  SLNK   L P FEGK K+K W+  L++M A DEL
Sbjct: 204 ITTMDSIRLKLVSVDGIYPLLSDLMESLNKNQWLGPTFEGKEKIKNWISILNQMKATDEL 263

Query: 182 TEQQARQLHFDLESSYNSF 201
            + Q+RQL FDL++SYN F
Sbjct: 264 DDDQSRQLLFDLDNSYNIF 282

BLAST of Bhi04G001565 vs. Swiss-Prot
Match: sp|Q9V359|VPS28_DROME (Vacuolar protein sorting-associated protein 28 homolog OS=Drosophila melanogaster OX=7227 GN=Vps28 PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 3.5e-37
Identity = 83/200 (41.50%), Postives = 122/200 (61.00%), Query Frame = 0

Query: 2   EVKLWNDKREREMYDNFVELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLAS 61
           EVKL+ + RERE YDN  +LYAII   ++LEKAYIRD I   +Y   C K +  +K    
Sbjct: 11  EVKLFRNAREREKYDNMADLYAIINTIQQLEKAYIRDCITPQEYTAACSKYLVQYKVAFK 70

Query: 62  TLK-DTVPSIELFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN 121
            ++ D  PS+E F   +++DCPAA+ R +    P T+                +AE V  
Sbjct: 71  QVQCDEFPSVETFVKKFRLDCPAALER-IREDRPITIR------DDKGNTSKCIAEIVSL 130

Query: 122 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMDAADE 181
           FIT MD L+L +  +D + P + DL+ ++N+L+++P DF+ K K+++WLG L++M A+DE
Sbjct: 131 FITIMDKLRLQINTMDALQPDVKDLADNMNRLSLIPEDFDAKLKVEKWLGSLNEMQASDE 190

Query: 182 LTEQQARQLHFDLESSYNSF 201
           L+E Q RQ  FDLES+Y  F
Sbjct: 191 LSEGQVRQFLFDLESAYADF 203

BLAST of Bhi04G001565 vs. Swiss-Prot
Match: sp|A8XWB7|VPS28_CAEBR (Vacuolar protein sorting-associated protein 28 homolog OS=Caenorhabditis briggsae OX=6238 GN=vps-28 PE=3 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 3.7e-34
Identity = 84/203 (41.38%), Postives = 121/203 (59.61%), Query Frame = 0

Query: 2   EVKLWNDKREREMYDNFVELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLAS 61
           EV+L+ +  ERE  +N  EL+A++ A E LEK + RD + + +Y+TECFKLI  +K +  
Sbjct: 10  EVRLFENHSEREQMENLSELFAVLNALEHLEKMFSRDHVTADEYKTECFKLIDQYK-VTM 69

Query: 62  TLKDTVPSIELFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQNF 121
            L     SIE FA  Y++ CPAAI R +  G P TV+               +A  V+ F
Sbjct: 70  RLVHGATSIEEFAKKYRLHCPAAIER-IREGRPITVKDDQGNVLKH------IASIVEQF 129

Query: 122 ITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMDAADEL 181
           IT +DSL+LN  AVD ++P+L DL  ++N  + +P D    TK+K+W  RLS M A DE+
Sbjct: 130 ITFLDSLRLNTRAVDDLYPVLDDLYNAINSTSRVPIDANVTTKVKKWHDRLSSMAATDEI 189

Query: 182 TEQQARQLHFDLESSYNSFMATL 205
           ++ +ARQ+ FD E +Y SF   L
Sbjct: 190 SDDEARQMIFDTEGAYQSFQKAL 204

BLAST of Bhi04G001565 vs. TAIR10
Match: AT4G05000.1 (Vacuolar protein sorting-associated protein VPS28 family protein)

HSP 1 Score: 337.0 bits (863), Expect = 8.1e-93
Identity = 170/208 (81.73%), Postives = 185/208 (88.94%), Query Frame = 0

Query: 1   MEVKLWNDKREREMYDNFVELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMY+NF EL+AIIKATEKLEKAYIRD+I  S+YE+EC KLI HFKTL+
Sbjct: 2   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDLINPSEYESECQKLIVHFKTLS 61

Query: 61  STLKDTVPSIELFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN 120
           +TLKDTVP+IE FADTYKMDCPAA+ RLV +G+PATVEHRA           IVAECVQN
Sbjct: 62  ATLKDTVPNIERFADTYKMDCPAALYRLVTSGLPATVEHRATVAASTSNSASIVAECVQN 121

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMDAADE 180
           FIT+MDSLKLNMVAVDQV+PLLSDLSASLNKL+ILPPDFEGKTKMKEWL RLSKM AADE
Sbjct: 122 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFEGKTKMKEWLSRLSKMGAADE 181

Query: 181 LTEQQARQLHFDLESSYNSFMATLPTAG 209
           LTEQQ+RQLHFDLESSYNSFMA LP AG
Sbjct: 182 LTEQQSRQLHFDLESSYNSFMAALPKAG 209

BLAST of Bhi04G001565 vs. TAIR10
Match: AT4G21560.1 (vacuolar protein sorting-associated protein 28 homolog 1)

HSP 1 Score: 329.7 bits (844), Expect = 1.3e-90
Identity = 167/208 (80.29%), Postives = 182/208 (87.50%), Query Frame = 0

Query: 1   MEVKLWNDKREREMYDNFVELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMY+NF ELYAIIKATEKLEKAYIRD+I  S+YETEC KLI HFKTL+
Sbjct: 1   MEVKLWNDKREREMYENFAELYAIIKATEKLEKAYIRDLISPSEYETECQKLIVHFKTLS 60

Query: 61  STLKDTVPSIELFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN 120
           ++LKD VP+IE FA+TYKMDC AA+ RLV +GVPATVEHRA           +VAECVQN
Sbjct: 61  ASLKDMVPNIERFAETYKMDCSAAVYRLVTSGVPATVEHRAAASASTSSSASVVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMDAADE 180
           FIT+MDSLKLNMVAVDQV+PLLSDLSASLNKL+ILPPDFEGK KMKEWL RLSKM A+DE
Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFEGKIKMKEWLLRLSKMGASDE 180

Query: 181 LTEQQARQLHFDLESSYNSFMATLPTAG 209
           LTEQQARQLHFDLESSYNSFMA LP AG
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPNAG 208

BLAST of Bhi04G001565 vs. TrEMBL
Match: tr|A0A1S3B0K8|A0A1S3B0K8_CUCME (Vacuolar protein sorting-associated protein 28 homolog OS=Cucumis melo OX=3656 GN=LOC103484547 PE=3 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 1.3e-100
Identity = 203/209 (97.13%), Postives = 204/209 (97.61%), Query Frame = 0

Query: 1   MEVKLWNDKREREMYDNFVELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMYDNF ELYAIIKATEKLEKAYIRDII SSDYETECFKLIAHFKTLA
Sbjct: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIISSSDYETECFKLIAHFKTLA 60

Query: 61  STLKDTVPSIELFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN 120
           STLKDTVPSIE FADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN
Sbjct: 61  STLKDTVPSIERFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMDAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDL+ASLNKLTILPPDFEGKTKMKEWLGRLSKM AADE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLAASLNKLTILPPDFEGKTKMKEWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMATLPTAGT 210
           LTEQQARQLHFDLESSYNSFMA LPTAGT
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPTAGT 209

BLAST of Bhi04G001565 vs. TrEMBL
Match: tr|A0A0A0KI07|A0A0A0KI07_CUCSA (Vacuolar protein sorting-associated protein 28 homolog OS=Cucumis sativus OX=3659 GN=Csa_6G517060 PE=3 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 2.2e-100
Identity = 203/209 (97.13%), Postives = 203/209 (97.13%), Query Frame = 0

Query: 1   MEVKLWNDKREREMYDNFVELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMYDNF ELYAIIKATEKLEKAYIRDII SSDYE ECFKLIAHFKTLA
Sbjct: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIISSSDYEAECFKLIAHFKTLA 60

Query: 61  STLKDTVPSIELFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN 120
           STLKDTVPSIE FADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN
Sbjct: 61  STLKDTVPSIERFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMDAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKM AADE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMATLPTAGT 210
           LTEQQARQLHFDLESSYNSFMA LPTAGT
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPTAGT 209

BLAST of Bhi04G001565 vs. TrEMBL
Match: tr|A0A1R3I226|A0A1R3I226_COCAP (Vacuolar protein sorting-associated protein 28 homolog OS=Corchorus capsularis OX=210143 GN=CCACVL1_15580 PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 2.5e-96
Identity = 195/209 (93.30%), Postives = 200/209 (95.69%), Query Frame = 0

Query: 1   MEVKLWNDKREREMYDNFVELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMY+NF ELYAIIKATEKLEKAY+RDII SS+YETEC KLIAHFKTLA
Sbjct: 6   MEVKLWNDKREREMYENFAELYAIIKATEKLEKAYVRDIISSSEYETECQKLIAHFKTLA 65

Query: 61  STLKDTVPSIELFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN 120
           STLKDTVPSIE FADTYKMDCPAAINRLV +GVPATVEHRAXXXXXXXXXXXIVAECVQN
Sbjct: 66  STLKDTVPSIERFADTYKMDCPAAINRLVTSGVPATVEHRAXXXXXXXXXXXIVAECVQN 125

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMDAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEW+ RLSKM AADE
Sbjct: 126 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWIARLSKMGAADE 185

Query: 181 LTEQQARQLHFDLESSYNSFMATLPTAGT 210
           LTEQQARQLHFDLESSYNSFMA LP AGT
Sbjct: 186 LTEQQARQLHFDLESSYNSFMAALPNAGT 214

BLAST of Bhi04G001565 vs. TrEMBL
Match: tr|A0A067JLK6|A0A067JLK6_JATCU (Vacuolar protein sorting-associated protein 28 homolog OS=Jatropha curcas OX=180498 GN=JCGZ_21366 PE=3 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 4.3e-96
Identity = 195/209 (93.30%), Postives = 200/209 (95.69%), Query Frame = 0

Query: 1   MEVKLWNDKREREMYDNFVELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMY+NF ELYAIIKATEKLEKAYIRDII SS+YETEC KLIAHFKTLA
Sbjct: 1   MEVKLWNDKREREMYENFAELYAIIKATEKLEKAYIRDIITSSEYETECHKLIAHFKTLA 60

Query: 61  STLKDTVPSIELFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN 120
           STLKD VPSIE FADTYKMDCPAAINRLV +GVPATVEHRAXXXXXXXXXXXIVAECVQN
Sbjct: 61  STLKDIVPSIERFADTYKMDCPAAINRLVTSGVPATVEHRAXXXXXXXXXXXIVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMDAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDLSASLNKL+ILPPDFEGKTKMKEW+ RLSKM AADE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLSILPPDFEGKTKMKEWISRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMATLPTAGT 210
           LTEQQARQLHFDLESSYNSFMA LPTAGT
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPTAGT 209

BLAST of Bhi04G001565 vs. TrEMBL
Match: tr|A0A061GEU0|A0A061GEU0_THECC (Vacuolar protein sorting-associated protein VPS28 family protein OS=Theobroma cacao OX=3641 GN=TCM_029974 PE=3 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 2.1e-95
Identity = 193/209 (92.34%), Postives = 200/209 (95.69%), Query Frame = 0

Query: 1   MEVKLWNDKREREMYDNFVELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMY+NF ELYAIIKATEKLEKAY+RDII SS+YETEC KLIAHFKTLA
Sbjct: 1   MEVKLWNDKREREMYENFAELYAIIKATEKLEKAYVRDIITSSEYETECQKLIAHFKTLA 60

Query: 61  STLKDTVPSIELFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN 120
           STLKDTVPSIE FADTYKMDCPAAINRLV +GVPATVEHRAXXXXXXXXXXXIVAECVQN
Sbjct: 61  STLKDTVPSIERFADTYKMDCPAAINRLVTSGVPATVEHRAXXXXXXXXXXXIVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMDAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDLSASLNKL+ILPPDFEGKTKMKEW+ RLSKM AADE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLSILPPDFEGKTKMKEWISRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMATLPTAGT 210
           LTEQQARQLHFDLESSYNSFMA LP AG+
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPNAGS 209

BLAST of Bhi04G001565 vs. NCBI nr
Match: XP_008439898.1 (PREDICTED: vacuolar protein sorting-associated protein 28 homolog 2 [Cucumis melo] >XP_016899348.1 PREDICTED: vacuolar protein sorting-associated protein 28 homolog 2 [Cucumis melo])

HSP 1 Score: 374.8 bits (961), Expect = 1.9e-100
Identity = 203/209 (97.13%), Postives = 204/209 (97.61%), Query Frame = 0

Query: 1   MEVKLWNDKREREMYDNFVELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMYDNF ELYAIIKATEKLEKAYIRDII SSDYETECFKLIAHFKTLA
Sbjct: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIISSSDYETECFKLIAHFKTLA 60

Query: 61  STLKDTVPSIELFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN 120
           STLKDTVPSIE FADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN
Sbjct: 61  STLKDTVPSIERFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMDAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDL+ASLNKLTILPPDFEGKTKMKEWLGRLSKM AADE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLAASLNKLTILPPDFEGKTKMKEWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMATLPTAGT 210
           LTEQQARQLHFDLESSYNSFMA LPTAGT
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPTAGT 209

BLAST of Bhi04G001565 vs. NCBI nr
Match: XP_022950166.1 (vacuolar protein sorting-associated protein 28 homolog 2 [Cucurbita moschata] >XP_022950167.1 vacuolar protein sorting-associated protein 28 homolog 2 [Cucurbita moschata] >XP_023544383.1 vacuolar protein sorting-associated protein 28 homolog 2 isoform X3 [Cucurbita pepo subsp. pepo] >XP_023544384.1 vacuolar protein sorting-associated protein 28 homolog 2 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 374.8 bits (961), Expect = 1.9e-100
Identity = 199/209 (95.22%), Postives = 201/209 (96.17%), Query Frame = 0

Query: 1   MEVKLWNDKREREMYDNFVELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMYDNF ELYAIIKATEKLEKAY+RDIIPSSDYETECFKLIAHFKTLA
Sbjct: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYVRDIIPSSDYETECFKLIAHFKTLA 60

Query: 61  STLKDTVPSIELFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN 120
           STLKDTVPSIE FADTYKMDCPAAINRLVI+GVPATVEHRA  XXXXXXXX IVAECVQN
Sbjct: 61  STLKDTVPSIERFADTYKMDCPAAINRLVISGVPATVEHRASAXXXXXXXXAIVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMDAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDL ASLNKLTILPPDFEGKTKMKEWLGRLSKM AADE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLFASLNKLTILPPDFEGKTKMKEWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMATLPTAGT 210
           LTEQQARQLHFDLESSYNSFMA LPTAGT
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPTAGT 209

BLAST of Bhi04G001565 vs. NCBI nr
Match: XP_023544381.1 (vacuolar protein sorting-associated protein 28 homolog 2 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023544382.1 vacuolar protein sorting-associated protein 28 homolog 2 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 374.8 bits (961), Expect = 1.9e-100
Identity = 199/209 (95.22%), Postives = 201/209 (96.17%), Query Frame = 0

Query: 1   MEVKLWNDKREREMYDNFVELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMYDNF ELYAIIKATEKLEKAY+RDIIPSSDYETECFKLIAHFKTLA
Sbjct: 37  MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYVRDIIPSSDYETECFKLIAHFKTLA 96

Query: 61  STLKDTVPSIELFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN 120
           STLKDTVPSIE FADTYKMDCPAAINRLVI+GVPATVEHRA  XXXXXXXX IVAECVQN
Sbjct: 97  STLKDTVPSIERFADTYKMDCPAAINRLVISGVPATVEHRASAXXXXXXXXAIVAECVQN 156

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMDAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDL ASLNKLTILPPDFEGKTKMKEWLGRLSKM AADE
Sbjct: 157 FITAMDSLKLNMVAVDQVHPLLSDLFASLNKLTILPPDFEGKTKMKEWLGRLSKMGAADE 216

Query: 181 LTEQQARQLHFDLESSYNSFMATLPTAGT 210
           LTEQQARQLHFDLESSYNSFMA LPTAGT
Sbjct: 217 LTEQQARQLHFDLESSYNSFMAALPTAGT 245

BLAST of Bhi04G001565 vs. NCBI nr
Match: XP_004134727.1 (PREDICTED: vacuolar protein sorting-associated protein 28 homolog 2 [Cucumis sativus] >KGN49198.1 hypothetical protein Csa_6G517060 [Cucumis sativus])

HSP 1 Score: 374.0 bits (959), Expect = 3.3e-100
Identity = 203/209 (97.13%), Postives = 203/209 (97.13%), Query Frame = 0

Query: 1   MEVKLWNDKREREMYDNFVELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMYDNF ELYAIIKATEKLEKAYIRDII SSDYE ECFKLIAHFKTLA
Sbjct: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIISSSDYEAECFKLIAHFKTLA 60

Query: 61  STLKDTVPSIELFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN 120
           STLKDTVPSIE FADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN
Sbjct: 61  STLKDTVPSIERFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMDAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKM AADE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMATLPTAGT 210
           LTEQQARQLHFDLESSYNSFMA LPTAGT
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPTAGT 209

BLAST of Bhi04G001565 vs. NCBI nr
Match: XP_022977932.1 (vacuolar protein sorting-associated protein 28 homolog 2 [Cucurbita maxima] >XP_022977933.1 vacuolar protein sorting-associated protein 28 homolog 2 [Cucurbita maxima])

HSP 1 Score: 371.7 bits (953), Expect = 1.6e-99
Identity = 198/209 (94.74%), Postives = 200/209 (95.69%), Query Frame = 0

Query: 1   MEVKLWNDKREREMYDNFVELYAIIKATEKLEKAYIRDIIPSSDYETECFKLIAHFKTLA 60
           MEVKLWNDKREREMYDNF ELYAIIKATEKLEKAY+RDII SSDYETECFKLIAHFKTLA
Sbjct: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYVRDIISSSDYETECFKLIAHFKTLA 60

Query: 61  STLKDTVPSIELFADTYKMDCPAAINRLVITGVPATVEHRAXXXXXXXXXXXIVAECVQN 120
           STLKDTVPSIE FADTYKMDCPAAINRLVI+GVPATVEHRA  XXXXXXXX IVAECVQN
Sbjct: 61  STLKDTVPSIERFADTYKMDCPAAINRLVISGVPATVEHRASAXXXXXXXXAIVAECVQN 120

Query: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLTILPPDFEGKTKMKEWLGRLSKMDAADE 180
           FITAMDSLKLNMVAVDQVHPLLSDL ASLNKLTILPPDFEGKTKMKEWLGRLSKM AADE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLFASLNKLTILPPDFEGKTKMKEWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMATLPTAGT 210
           LTEQQARQLHFDLESSYNSFMA LPTAGT
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPTAGT 209

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|Q9S9T7|VP282_ARATH1.5e-9181.73Vacuolar protein sorting-associated protein 28 homolog 2 OS=Arabidopsis thaliana... [more]
sp|O65421|VP281_ARATH2.3e-8980.29Vacuolar protein sorting-associated protein 28 homolog 1 OS=Arabidopsis thaliana... [more]
sp|Q54NF1|VPS28_DICDI1.2e-5053.27Vacuolar protein sorting-associated protein 28 OS=Dictyostelium discoideum OX=44... [more]
sp|Q9V359|VPS28_DROME3.5e-3741.50Vacuolar protein sorting-associated protein 28 homolog OS=Drosophila melanogaste... [more]
sp|A8XWB7|VPS28_CAEBR3.7e-3441.38Vacuolar protein sorting-associated protein 28 homolog OS=Caenorhabditis briggsa... [more]
Match NameE-valueIdentityDescription
AT4G05000.18.1e-9381.73Vacuolar protein sorting-associated protein VPS28 family protein[more]
AT4G21560.11.3e-9080.29vacuolar protein sorting-associated protein 28 homolog 1[more]
Match NameE-valueIdentityDescription
tr|A0A1S3B0K8|A0A1S3B0K8_CUCME1.3e-10097.13Vacuolar protein sorting-associated protein 28 homolog OS=Cucumis melo OX=3656 G... [more]
tr|A0A0A0KI07|A0A0A0KI07_CUCSA2.2e-10097.13Vacuolar protein sorting-associated protein 28 homolog OS=Cucumis sativus OX=365... [more]
tr|A0A1R3I226|A0A1R3I226_COCAP2.5e-9693.30Vacuolar protein sorting-associated protein 28 homolog OS=Corchorus capsularis O... [more]
tr|A0A067JLK6|A0A067JLK6_JATCU4.3e-9693.30Vacuolar protein sorting-associated protein 28 homolog OS=Jatropha curcas OX=180... [more]
tr|A0A061GEU0|A0A061GEU0_THECC2.1e-9592.34Vacuolar protein sorting-associated protein VPS28 family protein OS=Theobroma ca... [more]
Match NameE-valueIdentityDescription
XP_008439898.11.9e-10097.13PREDICTED: vacuolar protein sorting-associated protein 28 homolog 2 [Cucumis mel... [more]
XP_022950166.11.9e-10095.22vacuolar protein sorting-associated protein 28 homolog 2 [Cucurbita moschata] >X... [more]
XP_023544381.11.9e-10095.22vacuolar protein sorting-associated protein 28 homolog 2 isoform X1 [Cucurbita p... [more]
XP_004134727.13.3e-10097.13PREDICTED: vacuolar protein sorting-associated protein 28 homolog 2 [Cucumis sat... [more]
XP_022977932.11.6e-9994.74vacuolar protein sorting-associated protein 28 homolog 2 [Cucurbita maxima] >XP_... [more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0032509endosome transport via multivesicular body sorting pathway
Vocabulary: Cellular Component
TermDefinition
GO:0000813ESCRT I complex
Vocabulary: INTERPRO
TermDefinition
IPR037202ESCRT_assembly_dom
IPR017898VPS28_N
IPR017899VPS28_C
IPR038358VPS28_N_sf
IPR037206VPS28_C_sf
IPR007143Vps28
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043328 protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
biological_process GO:0032509 endosome transport via multivesicular body sorting pathway
cellular_component GO:0000813 ESCRT I complex
molecular_function GO:0044877 macromolecular complex binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi04M001565Bhi04M001565mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007143Vacuolar protein sorting-associated Vps28PFAMPF03997VPS28coord: 20..204
e-value: 8.3E-69
score: 231.0
IPR007143Vacuolar protein sorting-associated Vps28PIRSFPIRSF017535ESCRT1_Vps28coord: 1..205
e-value: 1.5E-78
score: 260.7
IPR007143Vacuolar protein sorting-associated Vps28PANTHERPTHR12937VACUOLAR PROTEIN SORTING 28, ISOFORM 2 VPS28coord: 2..206
IPR037206VPS28, C-terminal domain superfamilyGENE3DG3DSA:1.20.120.1130coord: 106..206
e-value: 1.1E-39
score: 136.4
IPR037206VPS28, C-terminal domain superfamilySUPERFAMILYSSF140427VPS28 C-terminal domain-likecoord: 110..204
IPR038358VPS28, N-terminal domain superfamilyGENE3DG3DSA:1.20.1440.200coord: 1..91
e-value: 8.6E-29
score: 101.8
IPR017899Vacuolar protein sorting-associated, VPS28, C-terminalPROSITEPS51310VPS28_Ccoord: 109..205
score: 44.592
IPR017898Vacuolar protein sorting-associated, VPS28, N-terminalPROSITEPS51313VPS28_Ncoord: 1..99
score: 30.049
IPR037202ESCRT assembly domainSUPERFAMILYSSF140111Endosomal sorting complex assembly domaincoord: 2..97