Bhi04G000526 (gene) Wax gourd

NameBhi04G000526
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionAMSH-like ubiquitin thioesterase
Locationchr4 : 14819491 .. 14819788 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGCCCGAGGCTTTCACCATTGTCATTGCTCCAACAGATGCATCAAGGTACCCACCCAAGTGCCAATTTGTGAAATTTAATTGTTGTGTCCAAGTTATAATTGAGATGGTGAAAGCCGATGTGGTTTAGGAGCTTTGGAATATTTCGACCGACTGATCCCGGTGGGATGAAGATCCTGAGAGAGTTTCAAGAGGATGGTTTTCACCCTCACAAAGATCCAGAAGATGGCAGCAACCCCATTTACGAGCTCCAACAATCTAACTTAAGATTTGAAATTATTGACTCGCGATCTTAA

mRNA sequence

ATGGTGCCCGAGGCTTTCACCATTGTCATTGCTCCAACAGATGCATCAAGGAGCTTTGGAATATTTCGACCGACTGATCCCGGTGGGATGAAGATCCTGAGAGAGTTTCAAGAGGATGGTTTTCACCCTCACAAAGATCCAGAAGATGGCAGCAACCCCATTTACGAGCTCCAACAATCTAACTTAAGATTTGAAATTATTGACTCGCGATCTTAA

Coding sequence (CDS)

ATGGTGCCCGAGGCTTTCACCATTGTCATTGCTCCAACAGATGCATCAAGGAGCTTTGGAATATTTCGACCGACTGATCCCGGTGGGATGAAGATCCTGAGAGAGTTTCAAGAGGATGGTTTTCACCCTCACAAAGATCCAGAAGATGGCAGCAACCCCATTTACGAGCTCCAACAATCTAACTTAAGATTTGAAATTATTGACTCGCGATCTTAA

Protein sequence

MVPEAFTIVIAPTDASRSFGIFRPTDPGGMKILREFQEDGFHPHKDPEDGSNPIYELQQSNLRFEIIDSRS
BLAST of Bhi04G000526 vs. Swiss-Prot
Match: sp|Q6NKP9|AMSH2_ARATH (AMSH-like ubiquitin thioesterase 2 OS=Arabidopsis thaliana OX=3702 GN=AMSH2 PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 3.5e-21
Identity = 49/75 (65.33%), Postives = 59/75 (78.67%), Query Frame = 0

Query: 1   MVPEAFTIVIAPTDASRSFGIFRPTDPGGMKILREFQEDGFHPHKDPEDGSNPIYE---- 60
           MVPEAF IV+APTD+S+S+GIF+ TDPGGM++LR   E GFHPHK+PEDG NP+YE    
Sbjct: 150 MVPEAFAIVVAPTDSSKSYGIFKLTDPGGMEVLRGCSETGFHPHKEPEDG-NPVYEHCSN 209

Query: 61  -LQQSNLRFEIIDSR 71
             + SNLRFEI D R
Sbjct: 210 VYKNSNLRFEIFDLR 223

BLAST of Bhi04G000526 vs. Swiss-Prot
Match: sp|Q8VYB5|AMSH1_ARATH (AMSH-like ubiquitin thioesterase 1 OS=Arabidopsis thaliana OX=3702 GN=AMSH1 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.7e-13
Identity = 38/75 (50.67%), Postives = 47/75 (62.67%), Query Frame = 0

Query: 1   MVPEAFTIVIAPTDASRSFGIFRPTDPGGMKILREFQEDGFHPHKDPEDGSNPIYE---- 60
           M+PEA  IV+AP D+SR+ GIFR T PGGM ++R     GFH H  PEDG  PIY     
Sbjct: 434 MLPEAVAIVMAPQDSSRNHGIFRLTTPGGMTVIRNCDRRGFHAHSSPEDG-GPIYNTCKE 493

Query: 61  -LQQSNLRFEIIDSR 71
                NL+F++ID R
Sbjct: 494 VYMNPNLKFDVIDLR 507

BLAST of Bhi04G000526 vs. Swiss-Prot
Match: sp|Q5PNU3|AMSH3_ARATH (AMSH-like ubiquitin thioesterase 3 OS=Arabidopsis thaliana OX=3702 GN=AMSH3 PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 3.5e-13
Identity = 36/75 (48.00%), Postives = 50/75 (66.67%), Query Frame = 0

Query: 1   MVPEAFTIVIAPTDASRSFGIFRPTDPGGMKILREFQEDGFHPHKDPEDGSNPIYE---- 60
           M+PEA  IV+APTD S   GIF  +DP G+ ++R  Q+ GFHPH++ EDG NPIYE    
Sbjct: 434 MLPEAVAIVMAPTDESTPHGIFHLSDPSGVSVIRNCQQRGFHPHEESEDG-NPIYEHCSH 493

Query: 61  -LQQSNLRFEIIDSR 71
               + L++E++D R
Sbjct: 494 VFLNAKLKYEVLDLR 507

BLAST of Bhi04G000526 vs. TAIR10
Match: AT1G10600.1 (associated molecule with the SH3 domain of STAM 2)

HSP 1 Score: 101.7 bits (252), Expect = 1.9e-22
Identity = 49/75 (65.33%), Postives = 59/75 (78.67%), Query Frame = 0

Query: 1   MVPEAFTIVIAPTDASRSFGIFRPTDPGGMKILREFQEDGFHPHKDPEDGSNPIYE---- 60
           MVPEAF IV+APTD+S+S+GIF+ TDPGGM++LR   E GFHPHK+PEDG NP+YE    
Sbjct: 150 MVPEAFAIVVAPTDSSKSYGIFKLTDPGGMEVLRGCSETGFHPHKEPEDG-NPVYEHCSN 209

Query: 61  -LQQSNLRFEIIDSR 71
             + SNLRFEI D R
Sbjct: 210 VYKNSNLRFEIFDLR 223

BLAST of Bhi04G000526 vs. TAIR10
Match: AT1G48790.1 (associated molecule with the SH3 domain of STAM 1)

HSP 1 Score: 75.5 bits (184), Expect = 1.5e-14
Identity = 38/75 (50.67%), Postives = 47/75 (62.67%), Query Frame = 0

Query: 1   MVPEAFTIVIAPTDASRSFGIFRPTDPGGMKILREFQEDGFHPHKDPEDGSNPIYE---- 60
           M+PEA  IV+AP D+SR+ GIFR T PGGM ++R     GFH H  PEDG  PIY     
Sbjct: 434 MLPEAVAIVMAPQDSSRNHGIFRLTTPGGMTVIRNCDRRGFHAHSSPEDG-GPIYNTCKE 493

Query: 61  -LQQSNLRFEIIDSR 71
                NL+F++ID R
Sbjct: 494 VYMNPNLKFDVIDLR 507

BLAST of Bhi04G000526 vs. TAIR10
Match: AT4G16144.1 (associated molecule with the SH3 domain of STAM 3)

HSP 1 Score: 75.1 bits (183), Expect = 1.9e-14
Identity = 36/75 (48.00%), Postives = 50/75 (66.67%), Query Frame = 0

Query: 1   MVPEAFTIVIAPTDASRSFGIFRPTDPGGMKILREFQEDGFHPHKDPEDGSNPIYE---- 60
           M+PEA  IV+APTD S   GIF  +DP G+ ++R  Q+ GFHPH++ EDG NPIYE    
Sbjct: 434 MLPEAVAIVMAPTDESTPHGIFHLSDPSGVSVIRNCQQRGFHPHEESEDG-NPIYEHCSH 493

Query: 61  -LQQSNLRFEIIDSR 71
               + L++E++D R
Sbjct: 494 VFLNAKLKYEVLDLR 507

BLAST of Bhi04G000526 vs. TrEMBL
Match: tr|M4DTS2|M4DTS2_BRARP (Uncharacterized protein OS=Brassica rapa subsp. pekinensis OX=51351 PE=4 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 2.4e-19
Identity = 51/75 (68.00%), Postives = 59/75 (78.67%), Query Frame = 0

Query: 1   MVPEAFTIVIAPTDASRSFGIFRPTDPGGMKILREFQEDGFHPHKDPEDGSNPIYE---- 60
           MVPEAF IV+APTD+SRS+GIF+ TDPGGM+ILR   E GFHPHK+PEDG NP+YE    
Sbjct: 151 MVPEAFAIVVAPTDSSRSYGIFKLTDPGGMEILRGCSETGFHPHKEPEDG-NPVYEQCSN 210

Query: 61  -LQQSNLRFEIIDSR 71
             + SNLRFEI D R
Sbjct: 211 VYKNSNLRFEIFDLR 224

BLAST of Bhi04G000526 vs. TrEMBL
Match: tr|W9RCX3|W9RCX3_9ROSA (AMSH-like ubiquitin thioesterase 2 OS=Morus notabilis OX=981085 GN=L484_027771 PE=4 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 7.1e-19
Identity = 50/75 (66.67%), Postives = 59/75 (78.67%), Query Frame = 0

Query: 1   MVPEAFTIVIAPTDASRSFGIFRPTDPGGMKILREFQEDGFHPHKDPEDGSNPIYE---- 60
           MVPEAF IV+APTD SRS+GIFR TDPGGM +LRE QE+GFHPHK+ E+GS P+YE    
Sbjct: 236 MVPEAFAIVMAPTDTSRSYGIFRLTDPGGMSVLRECQEEGFHPHKETENGS-PLYEHCDN 295

Query: 61  -LQQSNLRFEIIDSR 71
             + SN+RFEI D R
Sbjct: 296 VYKNSNIRFEIFDLR 309

BLAST of Bhi04G000526 vs. TrEMBL
Match: tr|A0A178W3T5|A0A178W3T5_ARATH (AMSH2 OS=Arabidopsis thaliana OX=3702 GN=AXX17_At1g10730 PE=4 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 7.1e-19
Identity = 49/75 (65.33%), Postives = 59/75 (78.67%), Query Frame = 0

Query: 1   MVPEAFTIVIAPTDASRSFGIFRPTDPGGMKILREFQEDGFHPHKDPEDGSNPIYE---- 60
           MVPEAF IV+APTD+S+S+GIF+ TDPGGM++LR   E GFHPHK+PEDG NP+YE    
Sbjct: 150 MVPEAFAIVVAPTDSSKSYGIFKLTDPGGMEVLRGCSETGFHPHKEPEDG-NPVYEHCSN 209

Query: 61  -LQQSNLRFEIIDSR 71
             + SNLRFEI D R
Sbjct: 210 VYKNSNLRFEIFDLR 223

BLAST of Bhi04G000526 vs. TrEMBL
Match: tr|A0A2P5FMB7|A0A2P5FMB7_9ROSA (JAB1/MPN/MOV34 metalloenzyme domain containing protein OS=Trema orientalis OX=63057 GN=TorRG33x02_051880 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.2e-18
Identity = 51/75 (68.00%), Postives = 58/75 (77.33%), Query Frame = 0

Query: 1   MVPEAFTIVIAPTDASRSFGIFRPTDPGGMKILREFQEDGFHPHKDPEDGSNPIYE---- 60
           MVPEAF IV+APTD SRS+GIFR TDPGGM +L+E QEDGFHPHK+  DGS+ IYE    
Sbjct: 198 MVPEAFAIVMAPTDTSRSYGIFRLTDPGGMTVLKECQEDGFHPHKETSDGSS-IYEHCNN 257

Query: 61  -LQQSNLRFEIIDSR 71
             + SNLRFEI D R
Sbjct: 258 VYKNSNLRFEIFDLR 271

BLAST of Bhi04G000526 vs. TrEMBL
Match: tr|R0GN48|R0GN48_9BRAS (Uncharacterized protein OS=Capsella rubella OX=81985 GN=CARUB_v10011162mg PE=4 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 1.6e-18
Identity = 48/75 (64.00%), Postives = 59/75 (78.67%), Query Frame = 0

Query: 1   MVPEAFTIVIAPTDASRSFGIFRPTDPGGMKILREFQEDGFHPHKDPEDGSNPIYE---- 60
           MVPEAF IV+APTD+S+S+GIF+ T+PGGM++LR   E GFHPHK+PEDG NP+YE    
Sbjct: 149 MVPEAFAIVVAPTDSSKSYGIFKLTNPGGMEVLRSCSETGFHPHKEPEDG-NPVYEHCSN 208

Query: 61  -LQQSNLRFEIIDSR 71
             + SNLRFEI D R
Sbjct: 209 VYKNSNLRFEIFDLR 222

BLAST of Bhi04G000526 vs. NCBI nr
Match: XP_023545741.1 (AMSH-like ubiquitin thioesterase 2 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 122.1 bits (305), Expect = 7.7e-25
Identity = 63/76 (82.89%), Postives = 65/76 (85.53%), Query Frame = 0

Query: 1   MVPEAFTIVIAPTDASRSFGIFRPTDPGGMKILREFQEDGFHPHKDPEDGSNPIYEL--- 60
           MVPEAF IV+APTDASRSFGIFR +DPGGMKILRE QEDGFHPHKDPEDGS PIYEL   
Sbjct: 191 MVPEAFAIVVAPTDASRSFGIFRLSDPGGMKILRECQEDGFHPHKDPEDGS-PIYELCSN 250

Query: 61  --QQSNLRFEIIDSRS 72
             Q SNLRFEIID RS
Sbjct: 251 VYQNSNLRFEIIDLRS 265

BLAST of Bhi04G000526 vs. NCBI nr
Match: XP_023545749.1 (AMSH-like ubiquitin thioesterase 2 isoform X8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 122.1 bits (305), Expect = 7.7e-25
Identity = 63/76 (82.89%), Postives = 65/76 (85.53%), Query Frame = 0

Query: 1   MVPEAFTIVIAPTDASRSFGIFRPTDPGGMKILREFQEDGFHPHKDPEDGSNPIYEL--- 60
           MVPEAF IV+APTDASRSFGIFR +DPGGMKILRE QEDGFHPHKDPEDGS PIYEL   
Sbjct: 170 MVPEAFAIVVAPTDASRSFGIFRLSDPGGMKILRECQEDGFHPHKDPEDGS-PIYELCSN 229

Query: 61  --QQSNLRFEIIDSRS 72
             Q SNLRFEIID RS
Sbjct: 230 VYQNSNLRFEIIDLRS 244

BLAST of Bhi04G000526 vs. NCBI nr
Match: XP_023545750.1 (AMSH-like ubiquitin thioesterase 2 isoform X9 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 122.1 bits (305), Expect = 7.7e-25
Identity = 63/76 (82.89%), Postives = 65/76 (85.53%), Query Frame = 0

Query: 1   MVPEAFTIVIAPTDASRSFGIFRPTDPGGMKILREFQEDGFHPHKDPEDGSNPIYEL--- 60
           MVPEAF IV+APTDASRSFGIFR +DPGGMKILRE QEDGFHPHKDPEDGS PIYEL   
Sbjct: 165 MVPEAFAIVVAPTDASRSFGIFRLSDPGGMKILRECQEDGFHPHKDPEDGS-PIYELCSN 224

Query: 61  --QQSNLRFEIIDSRS 72
             Q SNLRFEIID RS
Sbjct: 225 VYQNSNLRFEIIDLRS 239

BLAST of Bhi04G000526 vs. NCBI nr
Match: XP_023545745.1 (AMSH-like ubiquitin thioesterase 2 isoform X6 [Cucurbita pepo subsp. pepo] >XP_023545746.1 AMSH-like ubiquitin thioesterase 2 isoform X6 [Cucurbita pepo subsp. pepo] >XP_023545747.1 AMSH-like ubiquitin thioesterase 2 isoform X6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 122.1 bits (305), Expect = 7.7e-25
Identity = 63/76 (82.89%), Postives = 65/76 (85.53%), Query Frame = 0

Query: 1   MVPEAFTIVIAPTDASRSFGIFRPTDPGGMKILREFQEDGFHPHKDPEDGSNPIYEL--- 60
           MVPEAF IV+APTDASRSFGIFR +DPGGMKILRE QEDGFHPHKDPEDGS PIYEL   
Sbjct: 171 MVPEAFAIVVAPTDASRSFGIFRLSDPGGMKILRECQEDGFHPHKDPEDGS-PIYELCSN 230

Query: 61  --QQSNLRFEIIDSRS 72
             Q SNLRFEIID RS
Sbjct: 231 VYQNSNLRFEIIDLRS 245

BLAST of Bhi04G000526 vs. NCBI nr
Match: XP_023545743.1 (AMSH-like ubiquitin thioesterase 2 isoform X5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 122.1 bits (305), Expect = 7.7e-25
Identity = 63/76 (82.89%), Postives = 65/76 (85.53%), Query Frame = 0

Query: 1   MVPEAFTIVIAPTDASRSFGIFRPTDPGGMKILREFQEDGFHPHKDPEDGSNPIYEL--- 60
           MVPEAF IV+APTDASRSFGIFR +DPGGMKILRE QEDGFHPHKDPEDGS PIYEL   
Sbjct: 175 MVPEAFAIVVAPTDASRSFGIFRLSDPGGMKILRECQEDGFHPHKDPEDGS-PIYELCSN 234

Query: 61  --QQSNLRFEIIDSRS 72
             Q SNLRFEIID RS
Sbjct: 235 VYQNSNLRFEIIDLRS 249

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|Q6NKP9|AMSH2_ARATH3.5e-2165.33AMSH-like ubiquitin thioesterase 2 OS=Arabidopsis thaliana OX=3702 GN=AMSH2 PE=2... [more]
sp|Q8VYB5|AMSH1_ARATH2.7e-1350.67AMSH-like ubiquitin thioesterase 1 OS=Arabidopsis thaliana OX=3702 GN=AMSH1 PE=2... [more]
sp|Q5PNU3|AMSH3_ARATH3.5e-1348.00AMSH-like ubiquitin thioesterase 3 OS=Arabidopsis thaliana OX=3702 GN=AMSH3 PE=1... [more]
Match NameE-valueIdentityDescription
AT1G10600.11.9e-2265.33associated molecule with the SH3 domain of STAM 2[more]
AT1G48790.11.5e-1450.67associated molecule with the SH3 domain of STAM 1[more]
AT4G16144.11.9e-1448.00associated molecule with the SH3 domain of STAM 3[more]
Match NameE-valueIdentityDescription
tr|M4DTS2|M4DTS2_BRARP2.4e-1968.00Uncharacterized protein OS=Brassica rapa subsp. pekinensis OX=51351 PE=4 SV=1[more]
tr|W9RCX3|W9RCX3_9ROSA7.1e-1966.67AMSH-like ubiquitin thioesterase 2 OS=Morus notabilis OX=981085 GN=L484_027771 P... [more]
tr|A0A178W3T5|A0A178W3T5_ARATH7.1e-1965.33AMSH2 OS=Arabidopsis thaliana OX=3702 GN=AXX17_At1g10730 PE=4 SV=1[more]
tr|A0A2P5FMB7|A0A2P5FMB7_9ROSA1.2e-1868.00JAB1/MPN/MOV34 metalloenzyme domain containing protein OS=Trema orientalis OX=63... [more]
tr|R0GN48|R0GN48_9BRAS1.6e-1864.00Uncharacterized protein OS=Capsella rubella OX=81985 GN=CARUB_v10011162mg PE=4 S... [more]
Match NameE-valueIdentityDescription
XP_023545741.17.7e-2582.89AMSH-like ubiquitin thioesterase 2 isoform X3 [Cucurbita pepo subsp. pepo][more]
XP_023545749.17.7e-2582.89AMSH-like ubiquitin thioesterase 2 isoform X8 [Cucurbita pepo subsp. pepo][more]
XP_023545750.17.7e-2582.89AMSH-like ubiquitin thioesterase 2 isoform X9 [Cucurbita pepo subsp. pepo][more]
XP_023545745.17.7e-2582.89AMSH-like ubiquitin thioesterase 2 isoform X6 [Cucurbita pepo subsp. pepo] >XP_0... [more]
XP_023545743.17.7e-2582.89AMSH-like ubiquitin thioesterase 2 isoform X5 [Cucurbita pepo subsp. pepo][more]
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071108 protein K48-linked deubiquitination
biological_process GO:0070536 protein K63-linked deubiquitination
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi04M000526Bhi04M000526mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.40.140.10coord: 1..71
e-value: 5.3E-11
score: 44.1
NoneNo IPR availablePANTHERPTHR12947AMSHcoord: 1..70
NoneNo IPR availablePANTHERPTHR12947:SF9AMSH-LIKE UBIQUITIN THIOESTERASE 2coord: 1..70