Bhi04G000225 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi04G000225
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	tRNA wybutosine-synthesizing protein
Location	chr4 : 6072545 .. 6077982 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCTTTTCTTCGCGCTCCGCAAAGCTCACCCTTCTAGCTCTTCTCTCTGCTTCCACTCTGTATTGTTTCTACAAGTCCCGCCGTCTCAGAAAGCTTAAACTCTCCTTAAACCCTACTCTTTCTTCTCGCAGACCCAAACTGTTCTTTATTTCTCAAACTGGTACCTCCAAAGCCCTAGCTCATCGTCTGCTTAACGTTTTGAATTCCAATGAGTTGGTCTTTGATCTTGTCGATCCCAAGGATTATGAGCCTGAAGATCTTTTTAAGGAGACTTTAGTTTTGTTCGTTGCCTCCACTTGGGAAGACGGAGGACCTCCTCCTCATGCTAAGTTTCTCGCTAATTGGCTTTCTGAAAGCGCGGAGGATTTTAGAGTTGGTTCGTTGTTGCTTTCTGAGTGTAAATTTGCTGTGTTTGGTGTTGGTAGTCGAGCTTATGGAGAGACATTCAATGCGGTTGCAAGGAATTTTTCTAAGCGGATGAAGGCATTGGGTGCGAAGGAGATTTTAGCGGTTGGTAAAGGAGACGTTGATGGAGGTGATATAGATAGGTGTTTTGATGATTGGAGTCGGCAACTGCTGAAAGTTTTGAAGGTTGATGCGGTTGGAAATGGTGTGGATTTATATTCTGGGATTGGTGGGGACAGTGATGCTGAAAGTGTTGAGGAGGATGAAGAAGAAGATGCCGACCTTGGGGAGGAGGACATTGTTGATCTTGAGGATATTGCTGGGAAGGGTCCTTCGAGAAAGTCTACGAATGTAGTCGAAACTAATGGGAAATTGAATGGGAAAAAGGTTATGGTGACGCCGGTGATTAGAGCGAGCTTAGAAAAGCAGGTAAATTGTTTGAATAACTGTTTTTTAAAGGCAATTTCCGAGGGTCATGTGAATCAATACAAATTTGGACTGACTTGATAATTTGTCTTTGCAAAGGGCTAGTTGGGTTGGATTAGAGCCTCACCTCTTTTTCTTGTGTTGTTTGGTTGGCTCTTTGAATGGTTTTTCTTATGCAGGGATATAAAATTATTGGCTCACATAGCGGAGTGAAAATTTGTAGATGGACGAAGTCTCAGCTTCGAGGACGAGGAGGTTGCTATAAACATTCATTTTATGGCATAGAAAGTCATCGGTAATGTCCCACTTTACATGCTATTTTGCTTGGCCGTAGTGTGTCTTATTTCCTTTTCATGGTCCTTGTAATTCTTGATGTTTTAATGCATTAAGTGGCAATGGCATAGAGCTCCCCATTCCATAGATTAACATGTATGCCACTTTACTTTGGTAAATAATTAATTTCAGTTATTGAGGAGGATGGATGATAGAATATGATTGGATACTGGCTAGGTGAATAGCGAAAACAAGAATATAGTGGCAGTGACCGTCAAATCACTGCAGGATTCACAATATCCATTGTAGATCTTTATTTGGTTTGATATTCTATGCTCGGCCAATTTTTACATCCAGCTGAGTCTAATTTTATTTTTGGAATCGTTTGTTGTTCTGCCCGCGTGACTGATATCTTAAATTTCAACAATATCAGCAGAAAGTTTCATATTTTCTTTTCTTGTCTCCATACATCCTTGTTTTGTTGTTATGCTGGTGCAAGATGTGTGCTCAGAGGTCCTTTTGTTTGACTTGCATTATGAGTTCTTCTTCATCCTTGTTAAGTTTACTTCTCTGGCCCCTGGGAAATTTCAAGTTGACTCTTCACTTTCCTTTCACTCAACAAGGCTTTTCCTAAGCTCTTAACTTCTTTTAATAGACTGGTAAAAACTATCTATTGATTAGTGCTGTTGCCCACAGTAACTTTTCAATTTTGAGAACAGATGCATGGAGGCAACTCCCAGTTTGGCTTGTGCCAACAAATGTGTTTTCTGCTGGAGGCATCACACAAATCCAGTAGGAAAGAGTTGGCAGTGGGAGATGGATGATCCATTGGATATTGTGAATTCTGCAATCGATTTACACACAAAAATGATCAAGCAAATGAAAGGTGTTCCAGGTTAGTGCTCCTTCCTTACAGCTCTGGTTCGAGTAATAATCTAAGAAGCACGGATGAGGATATGACACAAATATGGACACGGTGACACATCATTTTTTTTTTAGAAAAAAAAAATTCTAGGATATGATATGACAAGGAAACAACGAACCAACAAAACTCATGCAGTAATCAATGCATTTCTATGACAGTCTTAAAAGAACATTTAACATTAACAATATTGATGATTTACCAAAAATTCAGATCCTACAATCAGCAAAACACGGGAACAATTGAGTCTGTCCAAGGAAATACATCCTTTAGCAACAATCTTGCAGTAATCAATGAAATGTCATTGATGAGTTTATGTTCACCTAAATCTTTTTATTTATTTATTTTTATATTATTTTATTTGGAAAGAAGTTCATGTCAACAAAATTTGACAGGCAATGACCAACGCTTGCTTACCGATGTGCAAAGCTTGTTCCTTCACAAAACAAGTGTAAAGCTAATTTTAGTTATTCAGATCCACTCATGTTAAGAAAACAAAAGTTCATGTACAATGCTAAAAAGTGAGATCTAGTTTTGCAACCCATGAATGGTTAAAGGTGTAAAAGCATATTTTCATTCATTGGTAGTTGGGATTTTAGTGATGAGGTGAGTCATTGATGCATGCCATCTTGTTGATAAGTGATGTCTTCCTGCAGGTGTTACGCAGGAGAAATTGGACGAAGGCCTGTCTCCAAGACATTGTGCCTTATCTCTTGTTGGTGAACCTATTATGTACCCAGAGATTAATACACTTGTTGATGAATTGCATCGAAGACGGATTTCAACTTTCTTGGTAACAAATGCTCAGTTTCCGGATAAAATAAAATTGCTGAAGCCAGTCACCCAGGTTTGCGTGCTTTACAAATTTAATTTTCTAAATAACATCAAATATTAGGCTTATACTCATGATACTAGATTACTTATCTGATTTCCATGATGCATGCAGTTGTATGTAAGTGTAGATGCTGCGACGAAGGAGAGCTTGAAGGCAATTGATAGACCACTTTTTGGAGACTTCTGGGAGAGATTCATTGTAAGTTTCCTCACACCCTCACCTCTTTCCTGGTCTGGTTCCTGAGCTAGGTTTCATCGGAATTAATGAGAAAAGTAAATCTTATGGAGGATGTTAAAATAAATAAGTTTAAAGTAAACTTTCCCTACCTCAAAACCTCATCTGGCCAATCACTTCTTGCCCACACTGGGTGCTCAATGGTCAAATACATCTACCTTACAATCTTGTTTGCGTGCCAAAGTAAAATTATTAAGCAACAAATGTGCCCCACTCTTCTAAAAAGGGGAAAAAAGGTATTCCTATCTTTAAGGAAGCATTTTATGACGAAGAGCAGGTAGTTTTAGTAATTTTCAGTTTGGCAATAAAAGTTTCATTAATTGGAGCAGGTTATTAGATGTGTTTGTGCCCTAAGTTTTCACAGAAAAGTCTCTGGCAGTATTCAGCCCCAATTACATATATTGTGTTTAATAACCATATTATCCTATTAACTTCTTCCTATGGTTATTTATCAGCAATTAAAATGATGGATTGCAAGAGAAGAGAATTTCAGGGCCTCTGGATGATTTGTGACTTGTGTGGTGTTTGATGAATTTGATTGCTTTTATATAATTAAATAAAAATACACACACACGTGCGTATAAATCATTGATATAAAACCAACCTGTGTGAAAGAACAACAAACAAAAAATGACTCACGAAAATAAGGGCTCGATCAAAAGAGAGGGGATGAGAATTAATACTCGAAAACATTTACAAGGAAGAACGTCAACCTTCCTGTAACCTAAAAAAATGAACAATTACCAAATAAATTTGAATTAAGATCCCAAAGAGAAGCCTAAACCCAAAACAAAAAATAATGACAAAATAAATTTGTATTAAGAGCCTAAAGAGAAGCCTAACTAAACACTGGATTCCACACTTCTCTTCAAGAGCTATCTCTTAGGATTACTCTTTTATTCCTTTCCAACTAGATAACCTACAAAACAACAGAAAGAGCCAGCTGGTCTGCCAAAGAACCCAACCTTGTTAGAAGTTAATTACATCGTTAGTATTATTTTAAAGGGCACATCATAAGCTGAGTGTTGATTCTTTTATGCAACTTATTAAATCATTGATATATCAATAAAATTTTATCATAAGTCATAATGTTAAACATAATATTTAGAAGGATAATACGGGATGCACCCATCTTTATGCATCCCCTCTCATCACTCACATAAAAAAGAAAAATAGTTAAAATATTTTAAAAAAATATAGATAATTGGACAATTTAATGCGATGCATGGAGATAGGTGCTCCCCAGCAGACACCTAAGTATTTTTCTATTTAGAAAAGCTACAAAAGATGCGATGCACATGACTTCATCACATCATTGCTGTATCTTTTTTGCTGACAGCTAAAAAGGTTCTTCAGCTTTATATCTTGTTGCTTTGTTGCCGTGACCTGCTTAGTAGTTTGGTAATCTTCTTTTTCATGTAGAAATGTGAAAGATTTGCAGTATCTTTACAAAAATCCTGAAAGACATTCTACTTTCTGGAGTACATTGTCAAAAAAGCCTGGATAAGGATCATATGTTGATTGTCAATTTGTTATCACACCAGAACTTAAGTTCTTTGATTATCACACTTAATCTTTCTGCCTCAAATTCTATGGACGGAAGGAAATATTCTTTAAATTACTGATTTATGTGCTTCCATAATGTTGCAGGATTCCTTGAAAGCTCTAAATGACAAGCAACAGCGAACAGTTTATCGATTGACGTTGGTAAAAGGGTGGAACACAGAAGATATTGATGCCTATTCAAATCTCTTTAGCATTGGTAGACCTGATTTTGTAGAAATCAAAGGCGTCACATATTGTGGATCGTGAGTTTATTTTTATTTATTTTGCATTAGGTGGGGAGGATATTGATTTGAAAATTGTGGGAGCTTAACTAGTCTAATGTTCTGTAGATCTAATACTTCAAAGCTGACAATGGAGAACGTTCCGTGGCATTCTGACGTCAAGTCTTTTTCTGAGGCTTTAGCTGCAAAAAGTCAAGGGGAGTATGAAGTTGCTTGTGAACATGTGCACTCATGTTGCGTTCTTTTGGCTAAAACTGACAAATTTAGGGTCGATGGCCAGTGGTTCACTTGGATAGATTATGAGAAATTCCATGACCTGGTTTGTATTGATGACAACTAATTTACCTGAAAGCTATAAGATGTTTAAGCTCCTTCCGTTAAATTTTATCCTTTTAAAAAATTTCAGGTGGCCTCTGGAAAACCTTTTGATAGCAAGGACTACATGGCTCCAACACCATCATGGGCTGTATATGGCTCCGATGAAGGTGGATTTGATCCTAATCAATCTCGGTACCGGAAGGAGCGGCATCATAGACCAAAACCCACATCCTGA

mRNA sequence

ATGTCCTTTTCTTCGCGCTCCGCAAAGCTCACCCTTCTAGCTCTTCTCTCTGCTTCCACTCTGTATTGTTTCTACAAGTCCCGCCGTCTCAGAAAGCTTAAACTCTCCTTAAACCCTACTCTTTCTTCTCGCAGACCCAAACTGTTCTTTATTTCTCAAACTGGTACCTCCAAAGCCCTAGCTCATCGTCTGCTTAACGTTTTGAATTCCAATGAGTTGGTCTTTGATCTTGTCGATCCCAAGGATTATGAGCCTGAAGATCTTTTTAAGGAGACTTTAGTTTTGTTCGTTGCCTCCACTTGGGAAGACGGAGGACCTCCTCCTCATGCTAAGTTTCTCGCTAATTGGCTTTCTGAAAGCGCGGAGGATTTTAGAGTTGGTTCGTTGTTGCTTTCTGAGTGTAAATTTGCTGTGTTTGGTGTTGGTAGTCGAGCTTATGGAGAGACATTCAATGCGGTTGCAAGGAATTTTTCTAAGCGGATGAAGGCATTGGGTGCGAAGGAGATTTTAGCGGTTGGTAAAGGAGACGTTGATGGAGGTGATATAGATAGGTGTTTTGATGATTGGAGTCGGCAACTGCTGAAAGTTTTGAAGGTTGATGCGGTTGGAAATGGTGTGGATTTATATTCTGGGATTGGTGGGGACAGTGATGCTGAAAGTGTTGAGGAGGATGAAGAAGAAGATGCCGACCTTGGGGAGGAGGACATTGTTGATCTTGAGGATATTGCTGGGAAGGGTCCTTCGAGAAAGTCTACGAATGTAGTCGAAACTAATGGGAAATTGAATGGGAAAAAGGTTATGGTGACGCCGGTGATTAGAGCGAGCTTAGAAAAGCAGGGATATAAAATTATTGGCTCACATAGCGGAGTGAAAATTTGTAGATGGACGAAGTCTCAGCTTCGAGGACGAGGAGGTTGCTATAAACATTCATTTTATGGCATAGAAAGTCATCGATGCATGGAGGCAACTCCCAGTTTGGCTTGTGCCAACAAATGTGTTTTCTGCTGGAGGCATCACACAAATCCAGTAGGAAAGAGTTGGCAGTGGGAGATGGATGATCCATTGGATATTGTGAATTCTGCAATCGATTTACACACAAAAATGATCAAGCAAATGAAAGGTGTTCCAGGTGTTACGCAGGAGAAATTGGACGAAGGCCTGTCTCCAAGACATTGTGCCTTATCTCTTGTTGGTGAACCTATTATGTACCCAGAGATTAATACACTTGTTGATGAATTGCATCGAAGACGGATTTCAACTTTCTTGGTAACAAATGCTCAGTTTCCGGATAAAATAAAATTGCTGAAGCCAGTCACCCAGTTGTATGTAAGTGTAGATGCTGCGACGAAGGAGAGCTTGAAGGCAATTGATAGACCACTTTTTGGAGACTTCTGGGAGAGATTCATTGATTCCTTGAAAGCTCTAAATGACAAGCAACAGCGAACAGTTTATCGATTGACGTTGGTAAAAGGGTGGAACACAGAAGATATTGATGCCTATTCAAATCTCTTTAGCATTGGTAGACCTGATTTTGTAGAAATCAAAGGCGTCACATATTGTGGATCATCTAATACTTCAAAGCTGACAATGGAGAACGTTCCGTGGCATTCTGACGTCAAGTCTTTTTCTGAGGCTTTAGCTGCAAAAAGTCAAGGGGAGTATGAAGTTGCTTGTGAACATGTGCACTCATGTTGCGTTCTTTTGGCTAAAACTGACAAATTTAGGGTCGATGGCCAGTGGTTCACTTGGATAGATTATGAGAAATTCCATGACCTGGTGGCCTCTGGAAAACCTTTTGATAGCAAGGACTACATGGCTCCAACACCATCATGGGCTGTATATGGCTCCGATGAAGGTGGATTTGATCCTAATCAATCTCGGTACCGGAAGGAGCGGCATCATAGACCAAAACCCACATCCTGA

Coding sequence (CDS)

ATGTCCTTTTCTTCGCGCTCCGCAAAGCTCACCCTTCTAGCTCTTCTCTCTGCTTCCACTCTGTATTGTTTCTACAAGTCCCGCCGTCTCAGAAAGCTTAAACTCTCCTTAAACCCTACTCTTTCTTCTCGCAGACCCAAACTGTTCTTTATTTCTCAAACTGGTACCTCCAAAGCCCTAGCTCATCGTCTGCTTAACGTTTTGAATTCCAATGAGTTGGTCTTTGATCTTGTCGATCCCAAGGATTATGAGCCTGAAGATCTTTTTAAGGAGACTTTAGTTTTGTTCGTTGCCTCCACTTGGGAAGACGGAGGACCTCCTCCTCATGCTAAGTTTCTCGCTAATTGGCTTTCTGAAAGCGCGGAGGATTTTAGAGTTGGTTCGTTGTTGCTTTCTGAGTGTAAATTTGCTGTGTTTGGTGTTGGTAGTCGAGCTTATGGAGAGACATTCAATGCGGTTGCAAGGAATTTTTCTAAGCGGATGAAGGCATTGGGTGCGAAGGAGATTTTAGCGGTTGGTAAAGGAGACGTTGATGGAGGTGATATAGATAGGTGTTTTGATGATTGGAGTCGGCAACTGCTGAAAGTTTTGAAGGTTGATGCGGTTGGAAATGGTGTGGATTTATATTCTGGGATTGGTGGGGACAGTGATGCTGAAAGTGTTGAGGAGGATGAAGAAGAAGATGCCGACCTTGGGGAGGAGGACATTGTTGATCTTGAGGATATTGCTGGGAAGGGTCCTTCGAGAAAGTCTACGAATGTAGTCGAAACTAATGGGAAATTGAATGGGAAAAAGGTTATGGTGACGCCGGTGATTAGAGCGAGCTTAGAAAAGCAGGGATATAAAATTATTGGCTCACATAGCGGAGTGAAAATTTGTAGATGGACGAAGTCTCAGCTTCGAGGACGAGGAGGTTGCTATAAACATTCATTTTATGGCATAGAAAGTCATCGATGCATGGAGGCAACTCCCAGTTTGGCTTGTGCCAACAAATGTGTTTTCTGCTGGAGGCATCACACAAATCCAGTAGGAAAGAGTTGGCAGTGGGAGATGGATGATCCATTGGATATTGTGAATTCTGCAATCGATTTACACACAAAAATGATCAAGCAAATGAAAGGTGTTCCAGGTGTTACGCAGGAGAAATTGGACGAAGGCCTGTCTCCAAGACATTGTGCCTTATCTCTTGTTGGTGAACCTATTATGTACCCAGAGATTAATACACTTGTTGATGAATTGCATCGAAGACGGATTTCAACTTTCTTGGTAACAAATGCTCAGTTTCCGGATAAAATAAAATTGCTGAAGCCAGTCACCCAGTTGTATGTAAGTGTAGATGCTGCGACGAAGGAGAGCTTGAAGGCAATTGATAGACCACTTTTTGGAGACTTCTGGGAGAGATTCATTGATTCCTTGAAAGCTCTAAATGACAAGCAACAGCGAACAGTTTATCGATTGACGTTGGTAAAAGGGTGGAACACAGAAGATATTGATGCCTATTCAAATCTCTTTAGCATTGGTAGACCTGATTTTGTAGAAATCAAAGGCGTCACATATTGTGGATCATCTAATACTTCAAAGCTGACAATGGAGAACGTTCCGTGGCATTCTGACGTCAAGTCTTTTTCTGAGGCTTTAGCTGCAAAAAGTCAAGGGGAGTATGAAGTTGCTTGTGAACATGTGCACTCATGTTGCGTTCTTTTGGCTAAAACTGACAAATTTAGGGTCGATGGCCAGTGGTTCACTTGGATAGATTATGAGAAATTCCATGACCTGGTGGCCTCTGGAAAACCTTTTGATAGCAAGGACTACATGGCTCCAACACCATCATGGGCTGTATATGGCTCCGATGAAGGTGGATTTGATCCTAATCAATCTCGGTACCGGAAGGAGCGGCATCATAGACCAAAACCCACATCCTGA

Protein sequence

MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTLSSRRPKLFFISQTGTSKALAHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSESAEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGKGDVDGGDIDRCFDDWSRQLLKVLKVDAVGNGVDLYSGIGGDSDAESVEEDEEEDADLGEEDIVDLEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPNQSRYRKERHHRPKPTS

Homology

[Close]

BLAST of Bhi04G000225 vs. Swiss-Prot
Match: sp|Q8RXN5|TYW1_ARATH (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Arabidopsis thaliana OX=3702 GN=TYW1 PE=2 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 5.7e-280
Identity = 479/644 (74.38%), Postives = 539/644 (83.70%), Query Frame = 0


Query: 4   SSRSAKLTLLALLSASTLYCFYKSRRLRKLK-LSLNP--TLSSRRPKLFFISQTGTSKAL 63
           SS   +L  +ALLSA+T YC +K RRL+ LK LSLNP  TL + R K+FFISQTGT+KAL
Sbjct: 5   SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64

Query: 64  AHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 123
           A RL  +  SN++ FD+VDP  YEPEDL KETLVLF+ASTW+ G PP + +FL NWL ES
Sbjct: 65  AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124

Query: 124 AEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGKGDVDGG 183
           AEDFRVGSLLLS+CKFAVFGVGSRAYGE++NAVA+  S RM  LG  E++ VG+GDVD G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184

Query: 184 DIDRCFDDWSRQLLKVLKVDAV--GNGVDLYSGIGGDSDAE----SVEEDEEEDADLGEE 243
           ++DR F DW   +++VLK  +    NGV    G   D D E    + EEDE+ DAD G  
Sbjct: 185 ELDRAFQDWCDGVIRVLKGGSAQETNGVSQQIGAVED-DLEYYDSTDEEDEDNDADGG-- 244

Query: 244 DIVDLEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICR 303
            IVDLEDIAGK PS+++  V  T  K++GKK MVTPVIRASL KQGYKIIGSHSGVKICR
Sbjct: 245 -IVDLEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICR 304

Query: 304 WTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDP 363
           WTKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MD+P
Sbjct: 305 WTKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEP 364

Query: 364 LDIVNSAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELH 423
             IV  A+DLH  MIKQMKGVPGVT EKL EGL+PRHCALSLVGEPIMYPEIN LVDELH
Sbjct: 365 SVIVKGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELH 424

Query: 424 RRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKA 483
            RRISTFLVTNAQFP+KI ++KP+TQLYVSVDAATKESLKAIDRPLF DFWERFIDSLKA
Sbjct: 425 GRRISTFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDSLKA 484

Query: 484 LNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVP 543
           L +KQQRTVYRLTLVKGWNTE++DAY NLFSIG+PDF+EIKGVTYCGSS TSKLTMENVP
Sbjct: 485 LQEKQQRTVYRLTLVKGWNTEELDAYFNLFSIGKPDFIEIKGVTYCGSSATSKLTMENVP 544

Query: 544 WHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVA 603
           WH+DVK+FSEAL+ KS GEYEVACEH HSCCVLL +T+KF+VDG+WFTWIDYEKFHDLVA
Sbjct: 545 WHTDVKAFSEALSLKSNGEYEVACEHAHSCCVLLGRTEKFKVDGKWFTWIDYEKFHDLVA 604

Query: 604 SGKPFDSKDYMAPTPSWAVYGSDEGGFDPNQSRYRKERHHRPKP 639
           SG+PF S DYMA TPSWAVYG+ EGGFDP Q RY+KER+H PKP
Sbjct: 605 SGEPFTSTDYMAQTPSWAVYGAQEGGFDPGQLRYKKERNHPPKP 642

[Close]

BLAST of Bhi04G000225 vs. Swiss-Prot
Match: sp|Q8H8N3|TYW1_ORYSJ (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Oryza sativa subsp. japonica OX=39947 GN=TYW1 PE=2 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 3.7e-239
Identity = 398/560 (71.07%), Postives = 459/560 (81.96%), Query Frame = 0


Query: 79  DPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSESAEDFRVGSLLLSECKFAV 138
           D   ++P+DL    L+L V  T + G     A FLA WL ESA DFR G+ LLS  +FAV
Sbjct: 89  DAAAFDPDDLPSLPLLLLVLPTHDGGAXXXXAAFLARWLEESAADFRAGAALLSGLRFAV 148

Query: 139 FGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGKGDVDGGDIDRCFDDWSRQLLKVLK 198
           FGVGSRAYGETFNA AR+FS+ ++ALGA E++AVG+GDVDGGD++  F++W  ++++V+K
Sbjct: 149 FGVGSRAYGETFNAAARSFSRWLRALGAAEVVAVGEGDVDGGDLEVVFEEWCGRVVRVVK 208

Query: 199 VDAVGNGVDLYSGIGGDSDAESVEEDEEEDADLGEEDIVDLEDIAGKGP-SRKSTNVVE- 258
            + +G G +  S   G  +                   VD+EDIAGK P +R+    VE 
Sbjct: 209 GEEIGEGHNGES--DGFDELXXXXXXXXXXXXXXXXGEVDMEDIAGKAPAARRRNGKVEG 268

Query: 259 --TNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGI 318
             +NG  NG + MVTP+IR SLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGI
Sbjct: 269 ALSNGGENGVRDMVTPIIRTSLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGI 328

Query: 319 ESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNSAIDLHTKMIKQMKG 378
           ESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+W+MDDPLDIVN+AID HTKM+KQMKG
Sbjct: 329 ESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDDPLDIVNAAIDQHTKMVKQMKG 388

Query: 379 VPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRISTFLVTNAQFPDKIKL 438
           VPGV  E+L EGLSPRHCALSLVGEPIMYPEIN L+DELHRR ISTFLVTNAQFPDKIK 
Sbjct: 389 VPGVKPERLAEGLSPRHCALSLVGEPIMYPEINVLIDELHRRHISTFLVTNAQFPDKIKT 448

Query: 439 LKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQRTVYRLTLVKGWNT 498
           LKP+TQLYVSVDAATKESLKA+DRPLF DFWERF+DSLK+L+DK QRTVYRLTLVKGWN 
Sbjct: 449 LKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLKSLHDKDQRTVYRLTLVKGWNA 508

Query: 499 EDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVKSFSEALAAKSQGEY 558
           E+ID Y+ L S+G+PDF+EIKGVTYCGSS TSKLTMENVPWHSDVK FSEALA KS G Y
Sbjct: 509 EEIDGYAKLLSLGQPDFIEIKGVTYCGSSATSKLTMENVPWHSDVKDFSEALALKSGGVY 568

Query: 559 EVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFDSKDYMAPTPSWAVY 618
           EVACEH HSCCVLLAK DKF+++G+W TWIDY++FH+LV SGKPF S+DYMA TPSWAVY
Sbjct: 569 EVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHELVTSGKPFRSQDYMALTPSWAVY 628

Query: 619 GSDEGGFDPNQSRYRKERHH 635
           G++EGGFDP+QSRY+KER H
Sbjct: 629 GAEEGGFDPDQSRYKKERRH 646

[Close]

BLAST of Bhi04G000225 vs. Swiss-Prot
Match: sp|Q9NV66|TYW1_HUMAN (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Homo sapiens OX=9606 GN=TYW1 PE=2 SV=2)

HSP 1 Score: 599.0 bits (1543), Expect = 6.3e-170
Identity = 307/646 (47.52%), Postives = 405/646 (62.69%), Query Frame = 0


Query: 47  KLFFISQTGTSKALAHRLLNVLNSNELVFDLVDPKDYEPED-----LFKETLVLFVASTW 106
           K+F+ SQTGT+K  A  L   + S +L   +++ K+Y+P+D     +  + + +F+ +T+
Sbjct: 80  KIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATY 139

Query: 107 EDGGPPPHAKFLANWLSESAEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRM 166
            DG P   A++   WL E++ DFR G   L   ++AVFG+G+ AY   FN V +N  K +
Sbjct: 140 TDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWL 199

Query: 167 KALGAKEILAVGKGDVD-----GGDIDRCFDDWSRQLLKVLKVDAVGNGVDLYSGIGGDS 226
             LGA  +++ G+GD D      G I+  F  W  + +  L+    G       G     
Sbjct: 200 WMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKG 259

Query: 227 DAESVEEDE--------------------------EEDADLGEED------IVDLEDIAG 286
             ES +                                   G ED      IVD+ED+  
Sbjct: 260 KCESHQHGSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGGEDHQSLNSIVDVEDLGK 319

Query: 287 -----KGPSRKSTNVVETNGKLNG--------KKVMVTPVIRASLEKQGYKIIGSHSGVK 346
                K   R      E +G            ++ M+TP +R +L KQGY++IGSHSGVK
Sbjct: 320 IMDHVKKEKRXXXXXXEKSGLFRNMGRNEDGERRAMITPALREALTKQGYQLIGSHSGVK 379

Query: 347 ICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEM 406
           +CRWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W+M
Sbjct: 380 LCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKM 439

Query: 407 DDPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVD 466
           D P  I+  AI+ H  MIKQ KGVPGV  E+ +EG++ +HCALSLVGEPIMYPEIN  + 
Sbjct: 440 DQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLK 499

Query: 467 ELHRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDS 526
            LH+ +IS+FLVTNAQFP +I+ L+PVTQLYVSVDA+TK+SLK IDRPLF DFW+RF+DS
Sbjct: 500 LLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDS 559

Query: 527 LKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTME 586
           LKAL  KQQRTVYRLTLVK WN +++ AY+ L S+G PDF+E+KGVTYCG S+ S LTM 
Sbjct: 560 LKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMA 619

Query: 587 NVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHD 632
           +VPWH +V  F   L      EYE+ACEH HS C+L+A   KF++ G+W+TWIDY +F +
Sbjct: 620 HVPWHEEVVQFVHELVDLIP-EYEIACEHEHSNCLLIAHR-KFKIGGEWWTWIDYNRFQE 679

[Close]

BLAST of Bhi04G000225 vs. Swiss-Prot
Match: sp|Q2KHP8|TYW1_XENLA (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Xenopus laevis OX=8355 GN=tyw1 PE=2 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 8.2e-170
Identity = 313/648 (48.30%), Postives = 413/648 (63.73%), Query Frame = 0


Query: 47  KLFFISQTGTSKALAHRLLNVLNSNELVFDLVDPKDYEPED-LFKET----LVLFVASTW 106
           K+F+ SQTGTSK  AH L   +    L  +L++ K+Y+P+D L +ET    + +F+ +T+
Sbjct: 84  KIFYGSQTGTSKGFAHLLAEEVTLLGLPVELINMKEYDPDDNLVEETTSKNICVFLVATY 143

Query: 107 EDGGPPPHAKFLANWLSESAEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRM 166
            DG PP  A++   WL E++ DFR G   L   ++AVFG+G+  Y   +N V +N  K +
Sbjct: 144 TDGKPPESAEWFCKWLEEASNDFRFGKTYLKGMRYAVFGLGNSVYSTHYNTVGKNIDKWL 203

Query: 167 KALGAKEILAVGKGDVD-----GGDIDRCFDDWSRQLLKVLKVDAVGNGVDLYSGIGGDS 226
             L A  ++   +GD +      G I+  F+ W R+ L  LK    G      SG     
Sbjct: 204 WMLSANRVMTRAEGDCNVVKSKHGSIESDFEAWKRKFLNRLKALLSGEKKPC-SGKCKKG 263

Query: 227 DAES--------------------------------VEEDEEEDADLGEEDIVDLEDIAG 286
             +S                                               ++D+ED+ G
Sbjct: 264 KCKSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGLIDVEDL-G 323

Query: 287 KGPS--RKSTNVVETNGKLNGKKV-------------MVTPVIRASLEKQGYKIIGSHSG 346
           K  S  +KS    ++N +L GK +             M+TP ++ +L KQGY++IGSHSG
Sbjct: 324 KAMSNMKKSKREHDSNTEL-GKSLQGDEAKEKEEPREMITPALQEALTKQGYRLIGSHSG 383

Query: 347 VKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQW 406
           VK+CRWTKS LRGRGGCYKH+FYGIESHRCME TPSLACANKCVFCWRHHTNPVG  W+W
Sbjct: 384 VKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRW 443

Query: 407 EMDDPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTL 466
           +MD P  I+  AI  H  MIKQ KGVPGV  E+ +EGL+ +HCALSLVGEPIMYPEINT 
Sbjct: 444 KMDQPEMILEEAILNHQNMIKQFKGVPGVKPERFEEGLAVKHCALSLVGEPIMYPEINTF 503

Query: 467 VDELHRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFI 526
           +  LH + IS+FLVTNAQFP++I+ L+PVTQLYVSVDA+TK+SLK IDRPLF DFW+RFI
Sbjct: 504 LRLLHNQHISSFLVTNAQFPEEIRSLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFI 563

Query: 527 DSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLT 586
           DSLKAL +KQQRTVYRLTLVK WN +++ AY++L ++G PDF+E+KGVTYCG S+ S LT
Sbjct: 564 DSLKALAEKQQRTVYRLTLVKAWNVDELKAYADLVALGLPDFIEVKGVTYCGESSASNLT 623

Query: 587 MENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKF 632
           M NVPWH +V  F E L      +YEVACEH HS C+L+A   KF+++G+W TWIDYE+F
Sbjct: 624 MANVPWHEEVIRFVEEL-VDLLPDYEVACEHEHSNCMLIAH-KKFKINGEWCTWIDYERF 683

[Close]

BLAST of Bhi04G000225 vs. Swiss-Prot
Match: sp|Q8BJM7|TYW1_MOUSE (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Mus musculus OX=10090 GN=Tyw1 PE=1 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 5.9e-168
Identity = 306/651 (47.00%), Postives = 399/651 (61.29%), Query Frame = 0


Query: 47  KLFFISQTGTSKALAHRLLNVLNSNELVFDLVDPKDYEPED-----LFKETLVLFVASTW 106
           K+F+ SQTGT+K  A  L   + S +L   +++ K+Y+P+D     +  +T+  F+ +T+
Sbjct: 72  KIFYGSQTGTAKGFAVVLAKAVTSLDLPVAIINLKEYDPDDSLIGEITSKTVCAFLVATY 131

Query: 107 EDGGPPPHAKFLANWLSESAEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRM 166
            DG P   A++   WL ESA DFR G   L   ++AVFG+G  AY   FN V+ N  K +
Sbjct: 132 TDGCPTESAEWFCKWLEESANDFRFGKTYLKGLRYAVFGLGDSAYRSHFNKVSTNVDKWL 191

Query: 167 KALGAKEILAVGKGDVDG-----GDIDRCFDDWSRQLLKVLKVDAVGNGVDLYSGIGGDS 226
             LGA+ +L  G+GD +      G I+  F  W  + +  L+    G         GG+ 
Sbjct: 192 WMLGAQRVLTRGEGDCNAVQSKHGSIEADFTAWKTKFISRLQALQRGE----KKACGGNC 251

Query: 227 DAESVEE---------------------------DEEEDADLGEEDIVDLEDIA------ 286
                E                                  +LG +D   L          
Sbjct: 252 KRGKCESAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXELGTQDYQSLTXXXXXXXXX 311

Query: 287 -----------------GKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGS 346
                            GK   +++    E        + M+TP +R +L KQGY++IGS
Sbjct: 312 XXXXXXXXXXXXXXXQDGKAAMQRNPEKTED----GEGRAMITPALREALTKQGYQLIGS 371

Query: 347 HSGVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKS 406
           HSGVK+CRWTKS LRGRGGCYKH+FYGIESHRCMEATPSLACANKCVFCWRHHTNPVG  
Sbjct: 372 HSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGTE 431

Query: 407 WQWEMDDPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEI 466
           W+W+MD P  I+  AI+ H  MIKQ KGVPG+  E+ +EG+  +HCALSLVGEPIMYPEI
Sbjct: 432 WRWKMDQPELILKEAIENHQNMIKQFKGVPGLKAERFEEGMEVKHCALSLVGEPIMYPEI 491

Query: 467 NTLVDELHRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWE 526
           N L+  LH+  IS+FLVTNAQFP++I+ L PVTQLYVSVDA+T++ LK IDRPLF DFW+
Sbjct: 492 NRLLKLLHQHGISSFLVTNAQFPEEIRKLTPVTQLYVSVDASTRDGLKKIDRPLFKDFWQ 551

Query: 527 RFIDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTS 586
           RF+DSLKAL+ KQQRTVYRLTLVK WN +++ AY+ L S+G PDF+E+KGVTYCG S  S
Sbjct: 552 RFLDSLKALSAKQQRTVYRLTLVKCWNVDELQAYAELVSLGNPDFIEVKGVTYCGESAAS 611

Query: 587 KLTMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDY 632
            LTM NVPWH +V  F   L      +YEVACEH HS C+L+    KF++DG+W+TWI+Y
Sbjct: 612 SLTMANVPWHEEVVRFVREL-VDLLPDYEVACEHEHSNCLLIGH-KKFKIDGEWWTWINY 671

[Close]

BLAST of Bhi04G000225 vs. TAIR10
Match: AT1G75200.1 (flavodoxin family protein / radical SAM domain-containing protein)

HSP 1 Score: 964.5 bits (2492), Expect = 3.2e-281
Identity = 479/644 (74.38%), Postives = 539/644 (83.70%), Query Frame = 0


Query: 4   SSRSAKLTLLALLSASTLYCFYKSRRLRKLK-LSLNP--TLSSRRPKLFFISQTGTSKAL 63
           SS   +L  +ALLSA+T YC +K RRL+ LK LSLNP  TL + R K+FFISQTGT+KAL
Sbjct: 5   SSVRVRLAFVALLSATTFYCIHKYRRLKHLKNLSLNPSSTLKASRGKIFFISQTGTAKAL 64

Query: 64  AHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 123
           A RL  +  SN++ FD+VDP  YEPEDL KETLVLF+ASTW+ G PP + +FL NWL ES
Sbjct: 65  AQRLHELCASNDIAFDIVDPHSYEPEDLPKETLVLFIASTWDGGKPPKNGEFLVNWLGES 124

Query: 124 AEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGKGDVDGG 183
           AEDFRVGSLLLS+CKFAVFGVGSRAYGE++NAVA+  S RM  LG  E++ VG+GDVD G
Sbjct: 125 AEDFRVGSLLLSDCKFAVFGVGSRAYGESYNAVAKELSSRMIGLGGLEMIPVGEGDVDDG 184

Query: 184 DIDRCFDDWSRQLLKVLKVDAV--GNGVDLYSGIGGDSDAE----SVEEDEEEDADLGEE 243
           ++DR F DW   +++VLK  +    NGV    G   D D E    + EEDE+ DAD G  
Sbjct: 185 ELDRAFQDWCDGVIRVLKGGSAQETNGVSQQIGAVED-DLEYYDSTDEEDEDNDADGG-- 244

Query: 244 DIVDLEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICR 303
            IVDLEDIAGK PS+++  V  T  K++GKK MVTPVIRASL KQGYKIIGSHSGVKICR
Sbjct: 245 -IVDLEDIAGKAPSKRNGVVKVT--KVDGKKEMVTPVIRASLTKQGYKIIGSHSGVKICR 304

Query: 304 WTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDP 363
           WTKSQLRGRGGCYKHSFYGIESHRCME TPSLACANKCVFCWRHHTNPVGKSWQW+MD+P
Sbjct: 305 WTKSQLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGKSWQWKMDEP 364

Query: 364 LDIVNSAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELH 423
             IV  A+DLH  MIKQMKGVPGVT EKL EGL+PRHCALSLVGEPIMYPEIN LVDELH
Sbjct: 365 SVIVKGALDLHKNMIKQMKGVPGVTPEKLQEGLNPRHCALSLVGEPIMYPEINALVDELH 424

Query: 424 RRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKA 483
            RRISTFLVTNAQFP+KI ++KP+TQLYVSVDAATKESLKAIDRPLF DFWERFIDSLKA
Sbjct: 425 GRRISTFLVTNAQFPEKILMMKPITQLYVSVDAATKESLKAIDRPLFADFWERFIDSLKA 484

Query: 484 LNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVP 543
           L +KQQRTVYRLTLVKGWNTE++DAY NLFSIG+PDF+EIKGVTYCGSS TSKLTMENVP
Sbjct: 485 LQEKQQRTVYRLTLVKGWNTEELDAYFNLFSIGKPDFIEIKGVTYCGSSATSKLTMENVP 544

Query: 544 WHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVA 603
           WH+DVK+FSEAL+ KS GEYEVACEH HSCCVLL +T+KF+VDG+WFTWIDYEKFHDLVA
Sbjct: 545 WHTDVKAFSEALSLKSNGEYEVACEHAHSCCVLLGRTEKFKVDGKWFTWIDYEKFHDLVA 604

Query: 604 SGKPFDSKDYMAPTPSWAVYGSDEGGFDPNQSRYRKERHHRPKP 639
           SG+PF S DYMA TPSWAVYG+ EGGFDP Q RY+KER+H PKP
Sbjct: 605 SGEPFTSTDYMAQTPSWAVYGAQEGGFDPGQLRYKKERNHPPKP 642

[Close]

BLAST of Bhi04G000225 vs. TrEMBL
Match: tr|A0A1S3BAY2|A0A1S3BAY2_CUCME (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Cucumis melo OX=3656 GN=LOC103487965 PE=4 SV=1)

HSP 1 Score: 1255.0 bits (3246), Expect = 0.0e+00
Identity = 612/641 (95.48%), Postives = 632/641 (98.60%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTLSSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKLT+LALLSASTLYCFYKSRRLRKLKLSLNPTLSSR+PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSN LVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGKGDVDGG 180
           AEDFRVGSLLLS+CKF+VFGVGSR YGET+NAVAR+FSKRMK+LGAKEIL +G+GDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180

Query: 181 DIDRCFDDWSRQLLKVLKVDAVGNGVDLYSGIGGDSDAESVEED-EEEDADLGEEDIVDL 240
           DID+CFDDWSR+LLKV+KVDA GNGV+L SGI GDSDA SVEE+ EEED DLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKL+EGLSPRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQ 480
           TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALN+KQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPNQSRYRKERHHRPKPTS 641
           DSKDY+APTPSWAVYGSDEGGFDP+QSRYRKERHH+PKPTS
Sbjct: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641

[Close]

BLAST of Bhi04G000225 vs. TrEMBL
Match: tr|A0A0A0LPA4|A0A0A0LPA4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G361620 PE=4 SV=1)

HSP 1 Score: 1237.2 bits (3200), Expect = 0.0e+00
Identity = 602/640 (94.06%), Postives = 621/640 (97.03%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTLSSRRPKLFFISQTGTSKAL 60
           MS SSRSAKLT+LALLSASTLYCFYKSRRLRKLKLSLNPTL SR+PKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSN LVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGKGDVDGG 180
           AEDFRVGSLLLS+CKF+VFGVGSR YGETFNAVAR+FSKR+KALGAKEIL +G+GDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180

Query: 181 DIDRCFDDWSRQLLKVLKVDAVGNGVDLYSGIGGDSDAESVEEDEEEDADLGEEDIVDLE 240
           DID+CFDDWSR+LLKV+K DA GNGV+L SGI GDSDA SVE        LGEEDIVDLE
Sbjct: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEXXXXXXXXLGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLHTKMIKQMKGVPGVTQEKL+EGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480
           FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALN+KQQ
Sbjct: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480

Query: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSS TSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD 600
           SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDY+KFHDLVASGKPFD
Sbjct: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPNQSRYRKERHHRPKPTS 641
           SKDYMAPTPSWAVYGSDEGGFDP+QSRYRKERHH+PKPTS
Sbjct: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 640

[Close]

BLAST of Bhi04G000225 vs. TrEMBL
Match: tr|A0A2I4EQG7|A0A2I4EQG7_9ROSI (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Juglans regia OX=51240 GN=LOC108991714 PE=4 SV=1)

HSP 1 Score: 1055.4 bits (2728), Expect = 5.0e-305
Identity = 518/645 (80.31%), Postives = 570/645 (88.37%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSL------NPTLSSRRPKLFFISQT 60
           MS SS  A+LTLLALLSAS+ YCFYKSRRLR+LK SL      NP LS  + K+FF+SQT
Sbjct: 1   MSLSSVPARLTLLALLSASSFYCFYKSRRLRQLKFSLNPIPYTNPNLSHSKAKIFFVSQT 60

Query: 61  GTSKALAHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLA 120
           GTS+ALA RL ++L+SN L FDLVDPKDYEPEDL KE+LVL +ASTWEDG PPP+AKF +
Sbjct: 61  GTSEALARRLFDLLSSNSLSFDLVDPKDYEPEDLPKESLVLIIASTWEDGKPPPNAKFFS 120

Query: 121 NWLSESAEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGK 180
           +WLSESAEDFRVGSLLLS CKFAVFGVGS AYG TFNAVAR++SK+M+ALGA EIL V +
Sbjct: 121 SWLSESAEDFRVGSLLLSRCKFAVFGVGSGAYGATFNAVARDYSKQMRALGANEILPVWE 180

Query: 181 GDVDGGDIDRCFDDWSRQLLKVLKVDAVGNGVDLYSG----IGGDSDAESVEEDEEEDAD 240
           GDVD GD D  F+ WS +++ +LKV  + NG  L S      GG SDAES    +E++  
Sbjct: 181 GDVDNGDFDEVFEAWSAKVVGLLKVGVLDNGAVLRSETEALAGGVSDAESFGSSDEDEDR 240

Query: 241 LGEEDIVDLEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGV 300
            G  +IVDLEDIAGKGPSRKST + E+NGKLNGKK MVTPVIRASLEKQGYKIIGSHSGV
Sbjct: 241 EGSAEIVDLEDIAGKGPSRKSTVMAESNGKLNGKKEMVTPVIRASLEKQGYKIIGSHSGV 300

Query: 301 KICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWE 360
           KICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQW+
Sbjct: 301 KICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWK 360

Query: 361 MDDPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLV 420
           MDDPL+IVN+AIDLHT MIKQMKGVPGV  E+L EGL+PRHCALSLVGEPIMYPEINTLV
Sbjct: 361 MDDPLEIVNTAIDLHTNMIKQMKGVPGVKLERLMEGLAPRHCALSLVGEPIMYPEINTLV 420

Query: 421 DELHRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFID 480
           DELHRRRISTFLVTNAQFP+KI+ LKPVTQLYVSVDA TKESLKAIDRPLFGDFWERFID
Sbjct: 421 DELHRRRISTFLVTNAQFPEKIEALKPVTQLYVSVDAGTKESLKAIDRPLFGDFWERFID 480

Query: 481 SLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTM 540
           SLKAL +KQQRTVYRLTLVKGWN EDIDAYSNLFSIG+PDFVEIKGVTYCGSS TSKLTM
Sbjct: 481 SLKALKEKQQRTVYRLTLVKGWNIEDIDAYSNLFSIGKPDFVEIKGVTYCGSSATSKLTM 540

Query: 541 ENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFH 600
           ENVPWHSDVK+FSE LA +SQGEYEVACEHVHSCCVLLA T+KF+V+G+WFTWIDYEKFH
Sbjct: 541 ENVPWHSDVKAFSETLALRSQGEYEVACEHVHSCCVLLANTEKFKVNGKWFTWIDYEKFH 600

Query: 601 DLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPNQSRYRKERHHR 636
           DLVASGKPFDS DYMA TPSWAVYG++EGGFDP+QSRY+KERHH+
Sbjct: 601 DLVASGKPFDSMDYMAATPSWAVYGAEEGGFDPDQSRYKKERHHK 645

[Close]

BLAST of Bhi04G000225 vs. TrEMBL
Match: tr|A0A2K2BPW6|A0A2K2BPW6_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_002G260700v3 PE=4 SV=1)

HSP 1 Score: 1047.0 bits (2706), Expect = 1.8e-302
Identity = 507/642 (78.97%), Postives = 569/642 (88.63%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTLSSRRPKLFFISQTGTSKAL 60
           M  +S  A+LTLLALLSA+T+YCFYKSRRL+K+ L+ NP     +PKLFFISQTGTSK L
Sbjct: 1   MPLTSTPARLTLLALLSATTIYCFYKSRRLKKISLNPNP----NKPKLFFISQTGTSKTL 60

Query: 61  AHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRL N+L SN L FDL+DP++YEPEDLFKE LV+ +ASTWEDG PP +AKF ANWL+ES
Sbjct: 61  AHRLHNLLASNNLHFDLIDPQNYEPEDLFKEKLVIIIASTWEDGKPPQNAKFFANWLAES 120

Query: 121 AEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGKGDVDGG 180
           A+DFRVGSL+LSECKFAVFGVGSRAYGETFNAVA++FS+R++ LGAKE++ VG+GDVDGG
Sbjct: 121 ADDFRVGSLMLSECKFAVFGVGSRAYGETFNAVAKDFSRRLRDLGAKEVVGVGEGDVDGG 180

Query: 181 DIDRCFDDWSRQLLKVLKVDAVGNGVDLYSGIGGDSDAESVEEDEEEDADLGEEDIVDLE 240
           ++D  F++WS +L +VLK ++V NG      + G+                 E DI+DLE
Sbjct: 181 ELDGVFEEWSGRLARVLKGESVENG-----EVCGNGVXXXXXXXXXXXXXXVESDIIDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNG-------KKVMVTPVIRASLEKQGYKIIGSHSGVKIC 300
           DIAGKGPSR+S  V ++NGKLNG        K MVTPVIRASLEKQGYKIIGSHSGVKIC
Sbjct: 241 DIAGKGPSRRSLAVAQSNGKLNGXXXXXXXXKEMVTPVIRASLEKQGYKIIGSHSGVKIC 300

Query: 301 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDD 360
           RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQW+MDD
Sbjct: 301 RWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWKMDD 360

Query: 361 PLDIVNSAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDEL 420
           PL+IVNSAIDLHTKMIKQMKGVPGVT E+L+EGLSPRHCALSLVGEPIMYPEINTLVDEL
Sbjct: 361 PLEIVNSAIDLHTKMIKQMKGVPGVTLERLNEGLSPRHCALSLVGEPIMYPEINTLVDEL 420

Query: 421 HRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLK 480
           HRR+ISTFLVTNAQFP+KI +LKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLK
Sbjct: 421 HRRQISTFLVTNAQFPEKILMLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLK 480

Query: 481 ALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENV 540
           +L +KQQRTVYRLTLVKGWNTED+DAY NLFS+G+PDF+EIKGVTYCGSS TSKLTMENV
Sbjct: 481 SLKEKQQRTVYRLTLVKGWNTEDLDAYFNLFSVGQPDFIEIKGVTYCGSSATSKLTMENV 540

Query: 541 PWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLV 600
           PWHSDVK+FSEA+A KS+GEYEVACEHVHSCCVLLAKT+KF+VDGQWFTWIDY+KFHDLV
Sbjct: 541 PWHSDVKAFSEAMALKSKGEYEVACEHVHSCCVLLAKTEKFKVDGQWFTWIDYDKFHDLV 600

Query: 601 ASGKPFDSKDYMAPTPSWAVYGSDEGGFDPNQSRYRKERHHR 636
           ASGKPFDSKDYMA TPSWAVYG+DEGGFDP+QSRYRKERHH+
Sbjct: 601 ASGKPFDSKDYMAATPSWAVYGADEGGFDPDQSRYRKERHHK 633

[Close]

BLAST of Bhi04G000225 vs. TrEMBL
Match: tr|A0A251IPB7|A0A251IPB7_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_18G027000 PE=4 SV=1)

HSP 1 Score: 1046.6 bits (2705), Expect = 2.3e-302
Identity = 508/647 (78.52%), Postives = 571/647 (88.25%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSL--------NPTLSSRRPKLFFIS 60
           MS SS  A+LTLLALLSAST YCFYKSRRL+ LK+SL        NP  SS + KLFFI+
Sbjct: 1   MSLSSTPARLTLLALLSASTFYCFYKSRRLKLLKVSLNAKDNLDANPVSSSNKGKLFFIT 60

Query: 61  QTGTSKALAHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKF 120
           QTGTSK LA RL N+L  N+L FDL+D KDYEPEDL KETLV+ +ASTWEDG PP +A F
Sbjct: 61  QTGTSKTLAQRLHNLLKLNDLPFDLIDIKDYEPEDLSKETLVIIIASTWEDGKPPSNASF 120

Query: 121 LANWLSESAEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAV 180
             NWL+ESA+DFRVGSLLLS+CKF+VFGVGS AYG+TFNAVA+ FS+R++ LGA EI+ V
Sbjct: 121 FGNWLAESADDFRVGSLLLSKCKFSVFGVGSAAYGDTFNAVAKGFSRRLRDLGANEIVEV 180

Query: 181 GKGDVDGGDIDRCFDDWSRQLLKVLKVDAVGNGVDLYSG-IGGDSDAESVEEDEEEDAD- 240
           G+GDVDGG++D  F++WS ++L++LK  +V NG    +G +GG+SD E++E+  E D D 
Sbjct: 181 GEGDVDGGELDLIFEEWSGKVLRILKGGSVQNGTVFSNGCVGGESDNENIEDGFESDNDG 240

Query: 241 --LGEEDIVDLEDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHS 300
               E  IVDLEDIAGKGPSR+ST V E N K+NG+K MVTPVIRA+LEKQGYKIIGSHS
Sbjct: 241 NGFAESGIVDLEDIAGKGPSRRSTAVAEVNEKVNGQKEMVTPVIRANLEKQGYKIIGSHS 300

Query: 301 GVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQ 360
           GVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK+WQ
Sbjct: 301 GVKICRWTKSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKNWQ 360

Query: 361 WEMDDPLDIVNSAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINT 420
           W+MDDPL+IVNSAID H KMIKQMKGVPGVT E+L EGLSPRHCALSLVGEPIMYPEIN 
Sbjct: 361 WKMDDPLEIVNSAIDQHAKMIKQMKGVPGVTLERLTEGLSPRHCALSLVGEPIMYPEINK 420

Query: 421 LVDELHRRRISTFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERF 480
           LVDELH+RRISTFLVTNAQFP+KIK+LKPVTQLYVSVDAATK+SLKAIDRPLFGDFWERF
Sbjct: 421 LVDELHQRRISTFLVTNAQFPEKIKMLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERF 480

Query: 481 IDSLKALNDKQQRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKL 540
           IDSLKAL +KQQRTVYRLTLVKGWNTED+DAY NLFSIG+PDF+EIKGVTYCG+S TSKL
Sbjct: 481 IDSLKALKEKQQRTVYRLTLVKGWNTEDVDAYFNLFSIGQPDFIEIKGVTYCGTSATSKL 540

Query: 541 TMENVPWHSDVKSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEK 600
           TMENVPWHSDVK+FSEALA KS+GEYEVACEHVHSCCVLLAKT+KF+V+GQWFTWIDY+K
Sbjct: 541 TMENVPWHSDVKAFSEALALKSKGEYEVACEHVHSCCVLLAKTEKFKVNGQWFTWIDYDK 600

Query: 601 FHDLVASGKPFDSKDYMAPTPSWAVYGSDEGGFDPNQSRYRKERHHR 636
           FHDLVASGKPF+ KDYMA TPSWAVYG+ EGGFDP+QSRYRKERHHR
Sbjct: 601 FHDLVASGKPFNGKDYMATTPSWAVYGAQEGGFDPDQSRYRKERHHR 647

[Close]

BLAST of Bhi04G000225 vs. NCBI nr
Match: XP_008444711.1 (PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis melo])

HSP 1 Score: 1255.0 bits (3246), Expect = 0.0e+00
Identity = 612/641 (95.48%), Postives = 632/641 (98.60%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTLSSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKLT+LALLSASTLYCFYKSRRLRKLKLSLNPTLSSR+PKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLSSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSN LVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGKGDVDGG 180
           AEDFRVGSLLLS+CKF+VFGVGSR YGET+NAVAR+FSKRMK+LGAKEIL +G+GDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETYNAVARDFSKRMKSLGAKEILPIGEGDVDGG 180

Query: 181 DIDRCFDDWSRQLLKVLKVDAVGNGVDLYSGIGGDSDAESVEED-EEEDADLGEEDIVDL 240
           DID+CFDDWSR+LLKV+KVDA GNGV+L SGI GDSDA SVEE+ EEED DLGEEDIVDL
Sbjct: 181 DIDKCFDDWSRKLLKVVKVDAEGNGVELCSGIAGDSDAVSVEEEYEEEDDDLGEEDIVDL 240

Query: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300
           EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ
Sbjct: 241 EDIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQ 300

Query: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360
           LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN
Sbjct: 301 LRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVN 360

Query: 361 SAIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIS 420
           SAIDLHTKMIKQMKGVPGVTQEKL+EGLSPRHCALSLVGEPIMYPEINTLVDELH+RRIS
Sbjct: 361 SAIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHQRRIS 420

Query: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQ 480
           TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALN+KQ
Sbjct: 421 TFLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQ 480

Query: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540
           QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV
Sbjct: 481 QRTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDV 540

Query: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600
           KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF
Sbjct: 541 KSFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPF 600

Query: 601 DSKDYMAPTPSWAVYGSDEGGFDPNQSRYRKERHHRPKPTS 641
           DSKDY+APTPSWAVYGSDEGGFDP+QSRYRKERHH+PKPTS
Sbjct: 601 DSKDYIAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 641

[Close]

BLAST of Bhi04G000225 vs. NCBI nr
Match: XP_004152698.1 (PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucumis sativus] >KGN62597.1 hypothetical protein Csa_2G361620 [Cucumis sativus])

HSP 1 Score: 1237.2 bits (3200), Expect = 0.0e+00
Identity = 602/640 (94.06%), Postives = 621/640 (97.03%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTLSSRRPKLFFISQTGTSKAL 60
           MS SSRSAKLT+LALLSASTLYCFYKSRRLRKLKLSLNPTL SR+PKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLTILALLSASTLYCFYKSRRLRKLKLSLNPTLPSRKPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSN LVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNGLVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGKGDVDGG 180
           AEDFRVGSLLLS+CKF+VFGVGSR YGETFNAVAR+FSKR+KALGAKEIL +G+GDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFSVFGVGSRVYGETFNAVARDFSKRLKALGAKEILPIGEGDVDGG 180

Query: 181 DIDRCFDDWSRQLLKVLKVDAVGNGVDLYSGIGGDSDAESVEEDEEEDADLGEEDIVDLE 240
           DID+CFDDWSR+LLKV+K DA GNGV+L SGI GDSDA SVE        LGEEDIVDLE
Sbjct: 181 DIDKCFDDWSRKLLKVMKFDAEGNGVELCSGIAGDSDAVSVEXXXXXXXXLGEEDIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTN+VETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNIVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPL+IVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLEIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLHTKMIKQMKGVPGVTQEKL+EGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480
           FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALN+KQQ
Sbjct: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNEKQQ 480

Query: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSS TSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSTTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD 600
           SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDY+KFHDLVASGKPFD
Sbjct: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYDKFHDLVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPNQSRYRKERHHRPKPTS 641
           SKDYMAPTPSWAVYGSDEGGFDP+QSRYRKERHH+PKPTS
Sbjct: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHKPKPTS 640

[Close]

BLAST of Bhi04G000225 vs. NCBI nr
Match: XP_022997485.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita maxima])

HSP 1 Score: 1229.5 bits (3180), Expect = 0.0e+00
Identity = 605/640 (94.53%), Postives = 615/640 (96.09%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTLSSRRPKLFFISQTGTSKAL 60
           MSFSSRSAKL LLALLSASTLYCFYKSRRLRKLKLSLNPT  SRRPKLFFISQTGTSKAL
Sbjct: 1   MSFSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNEL FD+VDPKD+EPEDLFKE LVLFVASTWEDGGPPPHAKFLANWLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKEALVLFVASTWEDGGPPPHAKFLANWLSES 120

Query: 121 AEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGKGDVDGG 180
           AEDFRVGSLLLS+CKFAVFGVGSRAYGETFNAVAR+FSKRMKALGAKEILAVG+GDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDRCFDDWSRQLLKVLKVDAVGNGVDLYSGIGGDSDAESVEEDEEEDADLGEEDIVDLE 240
           DID+CFDDWSRQLLKVLKVDAVG G DLYSGI  D DAESV E            IVDLE
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESV-EXXXXXXXXXXXXIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLHTKMIKQMKGVPGVTQEKL+EGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480
           FLVTNAQFPDKIK LKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ
Sbjct: 421 FLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480

Query: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWNTEDIDAYSNLFS+G+PDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD 600
           SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD
Sbjct: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPNQSRYRKERHHRPKPTS 641
           SKDYMAPTPSWAVYG+DEGGFDP QSRYRKERHHRPKPTS
Sbjct: 601 SKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPKPTS 639

[Close]

BLAST of Bhi04G000225 vs. NCBI nr
Match: XP_022144338.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Momordica charantia])

HSP 1 Score: 1224.2 bits (3166), Expect = 0.0e+00
Identity = 599/640 (93.59%), Postives = 617/640 (96.41%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTLSSRRPKLFFISQTGTSKAL 60
           MS SS SA+LTLLALLSAST Y FYKSRRLRKLKLSLNPT+ SRR KLFFIS+TGTSKAL
Sbjct: 1   MSMSSLSARLTLLALLSASTFYFFYKSRRLRKLKLSLNPTVCSRRAKLFFISETGTSKAL 60

Query: 61  AHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNEL FDLVDP+DYEPEDLFKE+LVL VASTWEDGGPPPHAKF ANWLSES
Sbjct: 61  AHRLLNVLNSNELAFDLVDPEDYEPEDLFKESLVLLVASTWEDGGPPPHAKFFANWLSES 120

Query: 121 AEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGKGDVDGG 180
           AEDFRVGSLLLSECKF VFGVGSRAYG+TFNAVAR FSK+M+ALGAKEIL VG+GDVDGG
Sbjct: 121 AEDFRVGSLLLSECKFGVFGVGSRAYGDTFNAVARGFSKQMRALGAKEILPVGEGDVDGG 180

Query: 181 DIDRCFDDWSRQLLKVLKVDAVGNGVDLYSGIGGDSDAESVEEDEEEDADLGEEDIVDLE 240
           DID+CFDDWS+Q+LKVLKVDAVG+G DLYS I GDSDAESV ED+EEDADLG EDIVDLE
Sbjct: 181 DIDKCFDDWSQQVLKVLKVDAVGSGTDLYSAIAGDSDAESV-EDDEEDADLGAEDIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGK PSRKSTNVVETNGKLNGKK MVTPVIRASLEKQGYKIIG+HSGVKICRWTKSQL
Sbjct: 241 DIAGKAPSRKSTNVVETNGKLNGKKEMVTPVIRASLEKQGYKIIGTHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLHTKMIKQMKGVPGVTQEKL EGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHTKMIKQMKGVPGVTQEKLAEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480
           FLVTNAQFPDKIK LKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ
Sbjct: 421 FLVTNAQFPDKIKSLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480

Query: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWN EDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNIEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD 600
           SFSEALAAKSQG+YEVACEHVHSCCVLLAKTDKFRV+GQWFTWIDYEKFHDLVASGKPFD
Sbjct: 541 SFSEALAAKSQGQYEVACEHVHSCCVLLAKTDKFRVNGQWFTWIDYEKFHDLVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPNQSRYRKERHHRPKPTS 641
           SKDYMAPTPSWAVYGSDEGGFDP+QSRYRKERHHRPKP S
Sbjct: 601 SKDYMAPTPSWAVYGSDEGGFDPDQSRYRKERHHRPKPMS 639

[Close]

BLAST of Bhi04G000225 vs. NCBI nr
Match: XP_022962153.1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita moschata])

HSP 1 Score: 1220.3 bits (3156), Expect = 0.0e+00
Identity = 600/640 (93.75%), Postives = 613/640 (95.78%), Query Frame = 0


Query: 1   MSFSSRSAKLTLLALLSASTLYCFYKSRRLRKLKLSLNPTLSSRRPKLFFISQTGTSKAL 60
           MS SSRSAKL LLALLSASTLYCFYKSRRLRKLKLSLNPT  SRRPKLFFISQTGTSKAL
Sbjct: 1   MSLSSRSAKLALLALLSASTLYCFYKSRRLRKLKLSLNPTGCSRRPKLFFISQTGTSKAL 60

Query: 61  AHRLLNVLNSNELVFDLVDPKDYEPEDLFKETLVLFVASTWEDGGPPPHAKFLANWLSES 120
           AHRLLNVLNSNEL FD+VDPKD+EPEDLFKETLVLFVASTWEDGGPPPHAKFLA+WLSES
Sbjct: 61  AHRLLNVLNSNELAFDIVDPKDFEPEDLFKETLVLFVASTWEDGGPPPHAKFLASWLSES 120

Query: 121 AEDFRVGSLLLSECKFAVFGVGSRAYGETFNAVARNFSKRMKALGAKEILAVGKGDVDGG 180
           AEDFRVGSLLLS+CKFAVFGVGSRAYGETFNAVAR+FSKRMKALGAKEILAVG+GDVDGG
Sbjct: 121 AEDFRVGSLLLSQCKFAVFGVGSRAYGETFNAVARDFSKRMKALGAKEILAVGEGDVDGG 180

Query: 181 DIDRCFDDWSRQLLKVLKVDAVGNGVDLYSGIGGDSDAESVEEDEEEDADLGEEDIVDLE 240
           DID+CFDDWSRQLLKVLKVDAVG G DLYSGI  D DAESV E            IVDLE
Sbjct: 181 DIDKCFDDWSRQLLKVLKVDAVGTGADLYSGIDEDFDAESV-EXXXXXXXXXXXXIVDLE 240

Query: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300
           DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL
Sbjct: 241 DIAGKGPSRKSTNVVETNGKLNGKKVMVTPVIRASLEKQGYKIIGSHSGVKICRWTKSQL 300

Query: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360
           RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS
Sbjct: 301 RGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWQWEMDDPLDIVNS 360

Query: 361 AIDLHTKMIKQMKGVPGVTQEKLDEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420
           AIDLH KMIKQMKGVPGVTQEKL+EGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST
Sbjct: 361 AIDLHKKMIKQMKGVPGVTQEKLEEGLSPRHCALSLVGEPIMYPEINTLVDELHRRRIST 420

Query: 421 FLVTNAQFPDKIKLLKPVTQLYVSVDAATKESLKAIDRPLFGDFWERFIDSLKALNDKQQ 480
           FLVTNAQFPDKIK LKPVTQLYVSVDAATK+SLKAIDRPLFGDFWERFIDSLKALNDKQQ
Sbjct: 421 FLVTNAQFPDKIKSLKPVTQLYVSVDAATKDSLKAIDRPLFGDFWERFIDSLKALNDKQQ 480

Query: 481 RTVYRLTLVKGWNTEDIDAYSNLFSIGRPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540
           RTVYRLTLVKGWNTEDIDAYSNLFS+G+PDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK
Sbjct: 481 RTVYRLTLVKGWNTEDIDAYSNLFSLGKPDFVEIKGVTYCGSSNTSKLTMENVPWHSDVK 540

Query: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRVDGQWFTWIDYEKFHDLVASGKPFD 600
           SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFR+DGQWFTWIDYEKFH LVASGKPFD
Sbjct: 541 SFSEALAAKSQGEYEVACEHVHSCCVLLAKTDKFRIDGQWFTWIDYEKFHALVASGKPFD 600

Query: 601 SKDYMAPTPSWAVYGSDEGGFDPNQSRYRKERHHRPKPTS 641
           SKDYMAPTPSWAVYG+DEGGFDP QSRYRKERHHRPKPTS
Sbjct: 601 SKDYMAPTPSWAVYGADEGGFDPVQSRYRKERHHRPKPTS 639

The following BLAST results are available for this feature:

BLAST of Bhi04G000225 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q8RXN5\|TYW1_ARATH	5.7e-280	74.38	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Arabidopsis...	[more]
sp\|Q8H8N3\|TYW1_ORYSJ	3.7e-239	71.07	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Oryza sativ...	[more]
sp\|Q9NV66\|TYW1_HUMAN	6.3e-170	47.52	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Homo sapien...	[more]
sp\|Q2KHP8\|TYW1_XENLA	8.2e-170	48.30	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Xenopus lae...	[more]
sp\|Q8BJM7\|TYW1_MOUSE	5.9e-168	47.00	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Mus musculu...	[more]

BLAST of Bhi04G000225 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G75200.1	3.2e-281	74.38	flavodoxin family protein / radical SAM domain-containing protein	[more]

BLAST of Bhi04G000225 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3BAY2\|A0A1S3BAY2_CUCME	0.0e+00	95.48	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Cucumis mel...	[more]
tr\|A0A0A0LPA4\|A0A0A0LPA4_CUCSA	0.0e+00	94.06	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G361620 PE=4 SV=1	[more]
tr\|A0A2I4EQG7\|A0A2I4EQG7_9ROSI	5.0e-305	80.31	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Juglans reg...	[more]
tr\|A0A2K2BPW6\|A0A2K2BPW6_POPTR	1.8e-302	78.97	Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_002G260700v3 PE=...	[more]
tr\|A0A251IPB7\|A0A251IPB7_MANES	2.3e-302	78.52	Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_18G027000 PE=4 SV=...	[more]

BLAST of Bhi04G000225 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008444711.1	0.0e+00	95.48	PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cu...	[more]
XP_004152698.1	0.0e+00	94.06	PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cu...	[more]
XP_022997485.1	0.0e+00	94.53	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita max...	[more]
XP_022144338.1	0.0e+00	93.59	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Momordica cha...	[more]
XP_022962153.1	0.0e+00	93.75	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Cucurbita mos...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0051539	4 iron, 4 sulfur cluster binding
GO:0003824	catalytic activity
GO:0051536	iron-sulfur cluster binding
GO:0010181	FMN binding

Vocabulary: **Biological Process**
Term	Definition
GO:0008033	tRNA processing
GO:0055114	oxidation-reduction process

Vocabulary: **INTERPRO**
Term	Definition
IPR034556	tRNA_wybutosine-synthase
IPR008254	Flavodoxin/NO_synth
IPR013917	tRNA_wybutosine-synth
IPR013785	Aldolase_TIM
IPR007197	rSAM
IPR029039	Flavoprotein-like_sf
IPR001094	Flavdoxin-like

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0016020	membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005768	endosome
cellular_component	GO:0005802	trans-Golgi network
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0003958	NADPH-hemoprotein reductase activity
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0010181	FMN binding
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0050661	NADP binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi04M000225	Bhi04M000225	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001094	Flavodoxin-like	PRINTS	PR00369	FLAVODOXIN	coord: 95..106 score: 39.97 coord: 133..143 score: 46.73 coord: 47..60 score: 35.6 coord: 158..177 score: 32.11
IPR029039	Flavoprotein-like superfamily	GENE3D	G3DSA:3.40.50.360		coord: 26..204 e-value: 5.7E-39 score: 135.4
IPR029039	Flavoprotein-like superfamily	SUPERFAMILY	SSF52218	Flavoproteins	coord: 47..198
IPR007197	Radical SAM	PFAM	PF04055	Radical_SAM	coord: 324..502 e-value: 3.3E-25 score: 89.3
IPR007197	Radical SAM	SFLD	SFLDS00029	Radical_SAM	coord: 324..501 e-value: 4.1E-29 score: 96.6
IPR013785	Aldolase-type TIM barrel	GENE3D	G3DSA:3.20.20.70		coord: 252..581 e-value: 6.6E-153 score: 510.9
IPR013917	tRNA wybutosine-synthesis	PFAM	PF08608	Wyosine_form	coord: 507..570 e-value: 1.4E-26 score: 92.5
IPR008254	Flavodoxin/nitric oxide synthase	PFAM	PF00258	Flavodoxin_1	coord: 48..189 e-value: 8.2E-25 score: 87.6
IPR008254	Flavodoxin/nitric oxide synthase	PROSITE	PS50902	FLAVODOXIN_LIKE	coord: 46..194 score: 21.853
None	No IPR available	SFLD	SFLDF00284	tRNA_wybutosine-synthesizing	coord: 263..575 e-value: 0.0 score: 487.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 612..640
None	No IPR available	PANTHER	PTHR13930:SF0	S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE	coord: 36..637
None	No IPR available	CDD	cd01335	Radical_SAM	coord: 324..496 e-value: 2.16261E-7 score: 49.6394
None	No IPR available	SUPERFAMILY	SSF102114	Radical SAM enzymes	coord: 325..504
IPR034556	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase	SFLD	SFLDG01071	tRNA_wybutosine-synthesizing	coord: 264..574 e-value: 0.0 score: 474.2
IPR034556	S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase	PANTHER	PTHR13930	RSAFD1-RELATED	coord: 36..637

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Bhi04G000225	Csa2G361620	Cucumber (Chinese Long) v2	cuwgoB162
Bhi04G000225	MELO3C010940	Melon (DHL92) v3.5.1	mewgoB322
Bhi04G000225	ClCG08G015110	Watermelon (Charleston Gray)	wcgwgoB612
Bhi04G000225	CSPI02G20010	Wild cucumber (PI 183967)	cpiwgoB159
Bhi04G000225	Cucsa.232190	Cucumber (Gy14) v1	cgywgoB458
Bhi04G000225	CmaCh05G002370	Cucurbita maxima (Rimu)	cmawgoB0943
Bhi04G000225	CmoCh05G002450	Cucurbita moschata (Rifu)	cmowgoB0944
Bhi04G000225	Lsi08G013980	Bottle gourd (USVL1VR-Ls)	lsiwgoB591
Bhi04G000225	Cp4.1LG11g01960	Cucurbita pepo (Zucchini)	cpewgoB0141
Bhi04G000225	CsGy2G019750	Cucumber (Gy14) v2	cgybwgoB157
Bhi04G000225	MELO3C010940.2	Melon (DHL92) v3.6.1	medwgoB321
Bhi04G000225	Carg11803	Silver-seed gourd	carwgoB0231
Bhi04G000225	CsaV3_2G028680	Cucumber (Chinese Long) v3	cucwgoB169
Bhi04G000225	Cla022496	Watermelon (97103) v1	wgowmB297
Bhi04G000225	Cla97C08G158680	Watermelon (97103) v2	wgowmbB313

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi04G000225	Cucumber (Gy14) v1	cgywgoB684
Bhi04G000225	Cucurbita maxima (Rimu)	cmawgoB0194
Bhi04G000225	Cucurbita maxima (Rimu)	cmawgoB0576
Bhi04G000225	Cucurbita moschata (Rifu)	cmowgoB0190
Bhi04G000225	Cucurbita moschata (Rifu)	cmowgoB0570
Bhi04G000225	Cucurbita pepo (Zucchini)	cpewgoB0684
Bhi04G000225	Cucurbita pepo (Zucchini)	cpewgoB1028
Bhi04G000225	Wild cucumber (PI 183967)	cpiwgoB319
Bhi04G000225	Cucumber (Chinese Long) v3	cucwgoB324
Bhi04G000225	Cucumber (Chinese Long) v2	cuwgoB318
Bhi04G000225	Watermelon (Charleston Gray)	wcgwgoB452
Bhi04G000225	Watermelon (97103) v2	wgowmbB301
Bhi04G000225	Watermelon (97103) v1	wgowmB329
Bhi04G000225	Silver-seed gourd	carwgoB1093
Bhi04G000225	Silver-seed gourd	carwgoB1143