Bhi04G000149 (gene) Wax gourd

BLAST of Bhi04G000149 vs. Swiss-Prot
Match: sp|B2HIN2|FAA26_MYCMM (Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium marinum (strain ATCC BAA-535 / M) OX=216594 GN=fadD26 PE=3 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 2.9e-50
Identity = 174/579 (30.05%), Postives = 271/579 (46.80%), Query Frame = 0

Query: 52  LTYQQLHDSVQFISDQLLRQLRRRDTVAVLCSAGLELVQLIYGCQRAGLVSVPISPPDPF 111
           LT+ Q++     ++D+L       D VA+L   GLE +    G  +AG + VP+S P   
Sbjct: 41  LTWSQVYARACVVADELTMYGVPGDRVAILAPQGLEYIVAFLGALQAGFIGVPLSTPQYG 100

Query: 112 LENENCHHLARALSQTKPRAAIAHQSYIYTIFRYLSSSPTDEKLALLLQSVQWISMDSLK 171
           + +E    ++  L  ++P A +   + +  + +Y SS   D + A  +  V  + +D+ +
Sbjct: 101 VHDE---RVSAVLRDSQPVAILTTSAVVGDVTKYASSQ--DGQPAPSVIEVDLLDLDTPR 160

Query: 172 QPDKKAEVNQDKHQPILYHSSSYYGCNPKEPYLIQYTSGATAIPKPVVVTAGAAAHNVRV 231
                    Q   QP    S S Y         +QYTSG+T  P  V+V+      NV  
Sbjct: 161 P-------QQALPQPA---SGSAY---------LQYTSGSTRTPAGVIVSHENVIANVTQ 220

Query: 232 ARKAYNLNP-----NDVIVSWLPQYHDCGLMF-LLLSVITGATCVLTSPMSFVTNPIRWL 291
           +   Y   P     +  +VSWLP +HD GL+  +   ++TG T VL SPMSF+  P RW+
Sbjct: 221 SLYGYFGGPDKFPADTTVVSWLPLFHDMGLILGICAPLVTGCTAVLLSPMSFLRRPARWM 280

Query: 292 HLITAFKATCTPVPSFTLPLVLKRVKDETPSSKGLDLRSLRNLILINEPIYRAAVEEFVD 351
            L+ +     +  P+F   L ++R  DE     GLDL  +  ++  +E I+ A ++ F +
Sbjct: 281 QLLASHPKCFSAAPNFAFELAVRRTTDE--DLAGLDLGDVLGIVSGSERIHVATIKRFTE 340

Query: 352 EFKAVGLDPGCVSPSYGLAENCTFVSTAWSGGRRWWFPAMPNY-RKLLPSGRLR----DG 411
            F    L P  V PSYGLAE   +V+    G      P    +  + L +G  R    DG
Sbjct: 341 RFAPFNLSPAAVRPSYGLAEATLYVAAPEPGTT----PRTVRFDYESLTAGHARPCRADG 400

Query: 412 -LCTEI---------KVVVVNGETGEVVEDGVEGEIWVSSPSNASGYLGHPSLTKKTFHS 471
            + TE+          V +VN ET      G  GEIW      A GY   P  + +TF++
Sbjct: 401 SVGTELISYGSPDPSAVRIVNPETMIENPSGTVGEIWAHGEHVAMGYWQKPEQSDRTFNA 460

Query: 472 KINNKS--SPN--FIRTGDRGVIRGADRFLFVIGRCSDLIKFNNTQQEIHPHYIESTAYN 531
           +I N +  +P   ++RTGD GV+   +  LF++GR  DL+  +      +P  IE+T   
Sbjct: 461 RIVNPAPGTPEGPWLRTGDLGVMSNGE--LFIMGRIKDLVIVDGRNH--YPDDIEATIQE 520

Query: 532 NSSAYLRGGCIAAV----KISETIAVVAEMQRDDKNDAEL---LRKICEEIRKAVSIEEG 591
                + GG +AA+     I+E +  + E++R   +  E    LR +  EI  A+S    
Sbjct: 521 -----ITGGRVAAIAVPDNITEQLVAIIELKRRGASAEEAMVKLRSVKREITSAISKSHS 580

Query: 592 TELGLVVLVKRGNIPKTTSGKVKRWAVKEKLAGGGLGVL 599
             +  VVLV  G+IP TTSGK++R A  E+    G   L
Sbjct: 581 LRVADVVLVPPGSIPITTSGKIRRAACVERYRSDGFNRL 580

BLAST of Bhi04G000149 vs. Swiss-Prot
Match: sp|B2HIL4|FAA29_MYCMM (4-hydroxyphenylalkanoate adenylyltransferase OS=Mycobacterium marinum (strain ATCC BAA-535 / M) OX=216594 GN=fadD29 PE=1 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 6.4e-50
Identity = 168/573 (29.32%), Postives = 259/573 (45.20%), Query Frame = 0

Query: 52  LTYQQLHDSVQFISDQLLRQLRRRDTVAVLCSAGLELVQLIYGCQRAGLVSVPISPPDPF 111
           LT+ Q++   + ++++L       D VAVL   GLE +    G   AGL++VP+  P   
Sbjct: 79  LTWWQIYRRAKIVAEELRGYGASGDRVAVLAPQGLEYIIAFLGVLEAGLIAVPLPVPQFG 138

Query: 112 LENENCHHLARALSQTKPRAAIAHQSYIYTIFRYLSSSPTDEKLALLLQSVQWISMDSLK 171
           + +E    ++ AL  + P   +     I  + +Y   +   +    ++ +V  + +DS +
Sbjct: 139 IHDE---RISAALQDSTPSVILTTSPVIDEVTKYAPHARAGQGGTPIVVAVDLLDLDSAR 198

Query: 172 QPDKKAEVNQDKHQPILYHSSSYYGCNPKEPYLIQYTSGATAIPKPVVVTAGAAAHNVRV 231
           + D               HSS+ Y         +QYTSG+T  P  VV++      N   
Sbjct: 199 ELDLTPPA----------HSSTAY---------LQYTSGSTRSPAGVVLSHKNVITNCVQ 258

Query: 232 ARKAYNLNPNDV---IVSWLPQYHDCGLMF-LLLSVITGATCVLTSPMSFVTNPIRWLHL 291
               Y      V    VSWLP YHD GLM  ++L +I   T VL +PM+F+  P RW+ L
Sbjct: 259 LMSDYLGETEKVPSTAVSWLPFYHDMGLMLGIILPMINQDTAVLLNPMAFLQRPARWMQL 318

Query: 292 ITAFKATCTPVPSFTLPLVLKRVKDETPSSKGLDLRSLRNLILINEPIYRAAVEEFVDEF 351
           +  F+   +  P+F   L ++R  DE     GLDL  +R +    E +  A ++ F+D F
Sbjct: 319 MGKFRGQISCAPNFGFELAVRRTSDE--DMAGLDLGHVRGIGSGAERVNPATLQRFIDRF 378

Query: 352 KAVGLDPGCVSPSYGLAENCTFVSTAWSGGRRWWFPAMPNY-RKLLPSGRLRD------- 411
               L    + PSYGLAE   FV+TA  G      P   N+  + L  GR+         
Sbjct: 379 APFNLRDTAIRPSYGLAEATVFVATAEPGRP----PRSVNFDYQSLSVGRVERCANEADD 438

Query: 412 -------GLCTEIKVVVVNGETGEVVEDGVEGEIWVSSPSNASGYLGHPSLTKKTFHSKI 471
                  G     +V +V+ E       G  GEIWV   + A GY  +P LT++TF +K+
Sbjct: 439 GAKLVSYGSSWTSEVRIVDPEARTECPAGTVGEIWVQGDNVAMGYWRNPQLTERTFDAKL 498

Query: 472 NNKSSPN----FIRTGDRGVIRGADRFLFVIGRCSDLIKFNNTQQEIHPHYIESTAYNNS 531
            + S       ++RTGD GV+   +  LF+ GR  DL+  + +    +P  IEST     
Sbjct: 499 TDPSPGTSIGPWLRTGDLGVMFEGE--LFITGRIKDLLIVDGSNH--YPDDIESTIQE-- 558

Query: 532 SAYLRGGCIAAVKISE-------TIAVVAEMQRDDKNDAELLRKICEEIRKAVSIEEGTE 591
              + GG + A+ + +       TI   A      +   + LR +  EI  A+S      
Sbjct: 559 ---ITGGRVVAIAVPDADGEKLVTIVEFASWGHSGQEAIDKLRSVKREITSAISRAHRVR 614

Query: 592 LGLVVLVKRGNIPKTTSGKVKRWAVKEKLAGGG 595
           +  VVLV  G+IP TTSGKV+R +  E+    G
Sbjct: 619 VADVVLVATGSIPVTTSGKVRRSSCAERYRNDG 614

BLAST of Bhi04G000149 vs. Swiss-Prot
Match: sp|Q7TXM1|FAA26_MYCBO (Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=fadD26 PE=1 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 8.3e-50
Identity = 171/574 (29.79%), Postives = 270/574 (47.04%), Query Frame = 0

Query: 52  LTYQQLHDSVQFISDQLLRQLRRRDTVAVLCSAGLELVQLIYGCQRAGLVSVPISPPDPF 111
           LT+ Q++     I+++L       D VAVL   GLE V    G  +AG ++VP+S P   
Sbjct: 41  LTWSQVYSRACIIAEELKLCGLPGDRVAVLAPQGLEYVLAFLGALQAGFIAVPLSTPQYG 100

Query: 112 LENENCHHLARALSQTKPRAAIAHQSYIYTIFRYLSSSPTDEKLALLLQSVQWISMDSLK 171
           + ++    ++  L  +KP A +   S +  + +Y +S   D + A ++  V  + +DS +
Sbjct: 101 IHDD---RVSAVLQDSKPVAILTTSSVVGDVTKYAASH--DGQPAPVVVEVDLLDLDSPR 160

Query: 172 QPDKKAEVNQDKHQPILYHSSSYYGCNPKEPYLIQYTSGATAIPKPVVVTAGAAAHNVRV 231
           Q    +            H+ + Y         +QYTSG+T  P  V+V+      NV  
Sbjct: 161 QMPAFSR----------QHTGAAY---------LQYTSGSTRTPAGVIVSHTNVIANVTQ 220

Query: 232 ARKAYNLNPNDV----IVSWLPQYHDCGLMF-LLLSVITGATCVLTSPMSFVTNPIRWLH 291
           +   Y  +P  +    +VSWLP YHD GL+  +   ++     VL SPMSF+  P RW+ 
Sbjct: 221 SMYGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAVLMSPMSFLRRPARWMQ 280

Query: 292 LITAFKATCTPVPSFTLPLVLKRVKDETPSSKGLDLRSLRNLILINEPIYRAAVEEFVDE 351
           L+       +  P+F   L ++R  D+     GLDLR +  ++  +E I+ A V  F++ 
Sbjct: 281 LLATSGRCFSAAPNFAFELAVRRTSDQ--DMAGLDLRDVVGIVSGSERIHVATVRRFIER 340

Query: 352 FKAVGLDPGCVSPSYGLAENCTFVSTAWSGGRRWWFPAMPNY-RKLLPSGRLR----DG- 411
           F    L P  + PSYGLAE   +V+   +G      P    +  + L +G+ R    DG 
Sbjct: 341 FAPYNLSPTAIRPSYGLAEATLYVAAPEAGAA----PKTVRFDYEQLTAGQARPCGTDGS 400

Query: 412 LCTEI---------KVVVVNGETGEVVEDGVEGEIWVSSPSNASGYLGHPSLTKKTFHSK 471
           + TE+          V +VN ET      GV GEIWV       GY   P  T + F +K
Sbjct: 401 VGTELISYGSPDPSSVRIVNPETMVENPPGVVGEIWVHGDHVTMGYWQKPKQTAQVFDAK 460

Query: 472 INN--KSSPN--FIRTGDRGVIRGADRFLFVIGRCSDLIKFNNTQQEIHPHYIESTAYNN 531
           + +   ++P   ++RTGD GVI  +D  LF++GR  DL+  +      +P  IE+T    
Sbjct: 461 LVDPAPAAPEGPWLRTGDLGVI--SDGELFIMGRIKDLLIVDGRNH--YPDDIEATIQE- 520

Query: 532 SSAYLRGGCIAAV----KISETIAVVAEMQRDDKNDAEL---LRKICEEIRKAVSIEEGT 591
               + GG  AA+     I+E +  + E +R      E+   LR +  E+  A+S     
Sbjct: 521 ----ITGGRAAAIAVPDDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSAISKSHSL 575

Query: 592 ELGLVVLVKRGNIPKTTSGKVKRWAVKEKLAGGG 595
            +  +VLV  G+IP TTSGK++R A  E+    G
Sbjct: 581 RVADLVLVSPGSIPITTSGKIRRSACVERYRSDG 575

BLAST of Bhi04G000149 vs. Swiss-Prot
Match: sp|P9WQ42|FAA26_MYCTO (Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=fadD26 PE=3 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 1.9e-49
Identity = 170/574 (29.62%), Postives = 270/574 (47.04%), Query Frame = 0

Query: 52  LTYQQLHDSVQFISDQLLRQLRRRDTVAVLCSAGLELVQLIYGCQRAGLVSVPISPPDPF 111
           LT+ Q++     I+++L       D VAVL   GLE V    G  +AG ++VP+S P   
Sbjct: 41  LTWSQVYSRACIIAEELKLCGLPGDRVAVLAPQGLEYVLAFLGALQAGFIAVPLSTPQYG 100

Query: 112 LENENCHHLARALSQTKPRAAIAHQSYIYTIFRYLSSSPTDEKLALLLQSVQWISMDSLK 171
           + ++    ++  L  +KP A +   S +  + +Y +S   D + A ++  V  + +DS +
Sbjct: 101 IHDD---RVSAVLQDSKPVAILTTSSVVGDVTKYAASH--DGQPAPVVVEVDLLDLDSPR 160

Query: 172 QPDKKAEVNQDKHQPILYHSSSYYGCNPKEPYLIQYTSGATAIPKPVVVTAGAAAHNVRV 231
           Q    +            H+ + Y         +QYTSG+T  P  V+V+      NV  
Sbjct: 161 QMPAFSR----------QHTGAAY---------LQYTSGSTRTPAGVIVSHTNVIANVTQ 220

Query: 232 ARKAYNLNPNDV----IVSWLPQYHDCGLMF-LLLSVITGATCVLTSPMSFVTNPIRWLH 291
           +   Y  +P  +    +VSWLP YHD GL+  +   ++     +L SPMSF+  P RW+ 
Sbjct: 221 SMYGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAMLMSPMSFLRRPARWMQ 280

Query: 292 LITAFKATCTPVPSFTLPLVLKRVKDETPSSKGLDLRSLRNLILINEPIYRAAVEEFVDE 351
           L+       +  P+F   L ++R  D+     GLDLR +  ++  +E I+ A V  F++ 
Sbjct: 281 LLATSGRCFSAAPNFAFELAVRRTSDQ--DMAGLDLRDVVGIVSGSERIHVATVRRFIER 340

Query: 352 FKAVGLDPGCVSPSYGLAENCTFVSTAWSGGRRWWFPAMPNY-RKLLPSGRLR----DG- 411
           F    L P  + PSYGLAE   +V+   +G      P    +  + L +G+ R    DG 
Sbjct: 341 FAPYNLSPTAIRPSYGLAEATLYVAAPEAGAA----PKTVRFDYEQLTAGQARPCGTDGS 400

Query: 412 LCTEI---------KVVVVNGETGEVVEDGVEGEIWVSSPSNASGYLGHPSLTKKTFHSK 471
           + TE+          V +VN ET      GV GEIWV       GY   P  T + F +K
Sbjct: 401 VGTELISYGSPDPSSVRIVNPETMVENPPGVVGEIWVHGDHVTMGYWQKPKQTAQVFDAK 460

Query: 472 INN--KSSPN--FIRTGDRGVIRGADRFLFVIGRCSDLIKFNNTQQEIHPHYIESTAYNN 531
           + +   ++P   ++RTGD GVI  +D  LF++GR  DL+  +      +P  IE+T    
Sbjct: 461 LVDPAPAAPEGPWLRTGDLGVI--SDGELFIMGRIKDLLIVDGRNH--YPDDIEATIQE- 520

Query: 532 SSAYLRGGCIAAV----KISETIAVVAEMQRDDKNDAEL---LRKICEEIRKAVSIEEGT 591
               + GG  AA+     I+E +  + E +R      E+   LR +  E+  A+S     
Sbjct: 521 ----ITGGRAAAIAVPDDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSAISKSHSL 575

Query: 592 ELGLVVLVKRGNIPKTTSGKVKRWAVKEKLAGGG 595
            +  +VLV  G+IP TTSGK++R A  E+    G
Sbjct: 581 RVADLVLVSPGSIPITTSGKIRRSACVERYRSDG 575

BLAST of Bhi04G000149 vs. Swiss-Prot
Match: sp|P9WQ43|FAA26_MYCTU (Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=fadD26 PE=1 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 1.9e-49
Identity = 170/574 (29.62%), Postives = 270/574 (47.04%), Query Frame = 0

Query: 52  LTYQQLHDSVQFISDQLLRQLRRRDTVAVLCSAGLELVQLIYGCQRAGLVSVPISPPDPF 111
           LT+ Q++     I+++L       D VAVL   GLE V    G  +AG ++VP+S P   
Sbjct: 41  LTWSQVYSRACIIAEELKLCGLPGDRVAVLAPQGLEYVLAFLGALQAGFIAVPLSTPQYG 100

Query: 112 LENENCHHLARALSQTKPRAAIAHQSYIYTIFRYLSSSPTDEKLALLLQSVQWISMDSLK 171
           + ++    ++  L  +KP A +   S +  + +Y +S   D + A ++  V  + +DS +
Sbjct: 101 IHDD---RVSAVLQDSKPVAILTTSSVVGDVTKYAASH--DGQPAPVVVEVDLLDLDSPR 160

Query: 172 QPDKKAEVNQDKHQPILYHSSSYYGCNPKEPYLIQYTSGATAIPKPVVVTAGAAAHNVRV 231
           Q    +            H+ + Y         +QYTSG+T  P  V+V+      NV  
Sbjct: 161 QMPAFSR----------QHTGAAY---------LQYTSGSTRTPAGVIVSHTNVIANVTQ 220

Query: 232 ARKAYNLNPNDV----IVSWLPQYHDCGLMF-LLLSVITGATCVLTSPMSFVTNPIRWLH 291
           +   Y  +P  +    +VSWLP YHD GL+  +   ++     +L SPMSF+  P RW+ 
Sbjct: 221 SMYGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAMLMSPMSFLRRPARWMQ 280

Query: 292 LITAFKATCTPVPSFTLPLVLKRVKDETPSSKGLDLRSLRNLILINEPIYRAAVEEFVDE 351
           L+       +  P+F   L ++R  D+     GLDLR +  ++  +E I+ A V  F++ 
Sbjct: 281 LLATSGRCFSAAPNFAFELAVRRTSDQ--DMAGLDLRDVVGIVSGSERIHVATVRRFIER 340

Query: 352 FKAVGLDPGCVSPSYGLAENCTFVSTAWSGGRRWWFPAMPNY-RKLLPSGRLR----DG- 411
           F    L P  + PSYGLAE   +V+   +G      P    +  + L +G+ R    DG 
Sbjct: 341 FAPYNLSPTAIRPSYGLAEATLYVAAPEAGAA----PKTVRFDYEQLTAGQARPCGTDGS 400

Query: 412 LCTEI---------KVVVVNGETGEVVEDGVEGEIWVSSPSNASGYLGHPSLTKKTFHSK 471
           + TE+          V +VN ET      GV GEIWV       GY   P  T + F +K
Sbjct: 401 VGTELISYGSPDPSSVRIVNPETMVENPPGVVGEIWVHGDHVTMGYWQKPKQTAQVFDAK 460

Query: 472 INN--KSSPN--FIRTGDRGVIRGADRFLFVIGRCSDLIKFNNTQQEIHPHYIESTAYNN 531
           + +   ++P   ++RTGD GVI  +D  LF++GR  DL+  +      +P  IE+T    
Sbjct: 461 LVDPAPAAPEGPWLRTGDLGVI--SDGELFIMGRIKDLLIVDGRNH--YPDDIEATIQE- 520

Query: 532 SSAYLRGGCIAAV----KISETIAVVAEMQRDDKNDAEL---LRKICEEIRKAVSIEEGT 591
               + GG  AA+     I+E +  + E +R      E+   LR +  E+  A+S     
Sbjct: 521 ----ITGGRAAAIAVPDDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSAISKSHSL 575

Query: 592 ELGLVVLVKRGNIPKTTSGKVKRWAVKEKLAGGG 595
            +  +VLV  G+IP TTSGK++R A  E+    G
Sbjct: 581 RVADLVLVSPGSIPITTSGKIRRSACVERYRSDG 575

BLAST of Bhi04G000149 vs. TAIR10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5)

HSP 1 Score: 80.5 bits (197), Expect = 4.1e-15
Identity = 98/405 (24.20%), Postives = 172/405 (42.47%), Query Frame = 0

Query: 198 NPKEPYLIQYTSGATAIPKPVVVT----AGAAAHNVRVARKAYNLNPNDVIVSWLPQYHD 257
           +P++   + Y+SG T +PK V++T      + A  V       N   NDVI+ +LP +H 
Sbjct: 208 SPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILCFLPMFHI 267

Query: 258 CGLMFLLLSVITGATCVLTSPMSFVTNPIRWLHLITAFKATCTPVPSFTLPLVLKRVKDE 317
             L  L+LS +     +L  P  F  N +  + LI  +K T  PV     P+VL  +K  
Sbjct: 268 YALDALMLSAMRTGAALLIVP-RFELNLV--MELIQRYKVTVVPVAP---PVVLAFIK-- 327

Query: 318 TPSSKGLDLRSLRNLILINEPIYRAAVEEFVDEFKAVGLDPGCVSPSYGLAENCTFVSTA 377
           +P ++  DL S+R ++        A +++ +++   +          YG+ E+ T   + 
Sbjct: 328 SPETERYDLSSVRIML-----SGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTVAKSL 387

Query: 378 WSGGRRWWFPAMPNYRKLLPSGRLRDGLCTEI----KVVVVNGETGEVVEDGVEGEIWVS 437
                   F   P         + + G C  +    ++ VV+ ETG  +     GEI V 
Sbjct: 388 -------AFAKNP--------FKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVR 447

Query: 438 SPSNASGYLGHPSLTKKTFHSKINNKSSPNFIRTGDRGVIRGADRFLFVIGRCSDLIKFN 497
                 GYL  P  T +T            ++ TGD G +   D  +F++ R  +LIKF 
Sbjct: 448 GHQLMKGYLNDPEATARTI-------DKDGWLHTGDIGFVDDDDE-IFIVDRLKELIKFK 507

Query: 498 NTQQEIHPHYIESTAYNNSSAYLRGGCIAAVKISET----IAVVAEMQRDDKNDAELLRK 557
             Q  + P  +E+   ++ S  +    + A+K        +A VA  Q     + ++   
Sbjct: 508 GYQ--VAPAELEALLISHPS--IDDAAVVAMKDEVADEVPVAFVARSQGSQLTEDDVKSY 564

Query: 558 ICEEIRKAVSIEEGTELGLVVLVKRGNIPKTTSGKVKRWAVKEKL 591
           + +++     I+      +V  ++   IPK  SGK+ R  ++ KL
Sbjct: 568 VNKQVVHYKRIK------MVFFIE--VIPKAVSGKILRKDLRAKL 564

BLAST of Bhi04G000149 vs. TAIR10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 63.5 bits (153), Expect = 5.2e-10
Identity = 80/290 (27.59%), Postives = 118/290 (40.69%), Query Frame = 0

Query: 207 YTSGATAIPKPVVVTAG---AAAHNVRVARKAYNLNPNDVIVSWLPQYHDCGLMFLLLSV 266
           Y+SG T   K V +T G   AA+  V + +       + V + +LP +H  GL  +  S 
Sbjct: 197 YSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMG-EYHGVFLCFLPMFHVFGLAVITYSQ 256

Query: 267 ITGATCVLTSPMSFVTNPIRWLHLITAFKAT-CTPVPSFTLPLVLKRVKDETPSSKGLDL 326
           +      L S   F    +  L  I  F+ T    VP   L L  + +       K  DL
Sbjct: 257 LQRGN-ALVSMARFELELV--LKNIEKFRVTHLWVVPPVFLALSKQSI------VKKFDL 316

Query: 327 RSLRNLILINEPIYRAAVEEFVDEFKAVGLDPGCVSPSYGLAENCTFVSTAWSGGRRWWF 386
            SL+ +     P+ +  +EE       V L  G     YG+ E C  VS           
Sbjct: 317 SSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQG-----YGMTETCGIVSVE--------- 376

Query: 387 PAMPNYRKLLPSGRLRDGLCTEIKVVVVNGETGEVVEDGVEGEIWVSSPSNASGYLGHPS 446
                 R    +G L  G    ++  +V+ ETG+      +GEIWV  P+   GYL +P 
Sbjct: 377 DPRLGKRNSGSAGMLAPG----VEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQ 436

Query: 447 LTKKTFHSKINNKSSPNFIRTGDRGVIRGADRFLFVIGRCSDLIKFNNTQ 493
            TK+T   K       +++ TGD G     D  L+V+ R  +LIK+   Q
Sbjct: 437 ATKETIDKK-------SWVHTGDLGYF-NEDGNLYVVDRIKELIKYKGFQ 450

BLAST of Bhi04G000149 vs. TAIR10
Match: AT3G48990.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 62.8 bits (151), Expect = 8.8e-10
Identity = 93/394 (23.60%), Postives = 163/394 (41.37%), Query Frame = 0

Query: 198 NPKEPYLIQYTSGATAIPKPVVVTAGAAAHNVRVARKAYNLNPNDVIVSWLPQYHDCGLM 257
           +P +  L  +TSG T+ PK V +T    A +V+  +  Y L  +D  V  LP +H  GL+
Sbjct: 160 HPDDGALFLHTSGTTSRPKGVPLTQLNLASSVKNIKAVYKLTESDSTVIVLPLFHVHGLL 219

Query: 258 FLLLSVITGATCVLTSPMSFVTNPIRWLHLITAFKAT-CTPVPSFTLPLVLKRVKDETPS 317
             LLS + GA   +T P +   +   +   +  + AT  T VP  T+  ++       P 
Sbjct: 220 AGLLSSL-GAGAAVTLPAAGRFSATTFWPDMKKYNATWYTAVP--TIHQIILDRHASHPE 279

Query: 318 SKGLDLRSLRNLILINEPIYRAAVEEFVDEFKAVGLDPGCVSPSYGLAENCTFVSTAWSG 377
           ++   LR +R+      P+  + +EE      A G     V  +Y + E    +S+    
Sbjct: 280 TEYPKLRFIRSCSASLAPVILSRLEE------AFGAP---VLEAYAMTEATHLMSS---- 339

Query: 378 GRRWWFPAMPNYRKLLPSGRLRDGLCTEIKVVVVNGETGEVVEDGVEGEIWVSSPSNASG 437
                   +P      P G +   +  E+ ++    E GE+ E   +GE+ +  P+   G
Sbjct: 340 ------NPLPEEGPHKP-GSVGKPVGQEMAIL---NEKGEIQEPNNKGEVCIRGPNVTKG 399

Query: 438 YLGHPSLTKKTFHSKINNKSSPNFIRTGDRGVIRGADRFLFVIGRCSDLIKFNNTQQEIH 497
           Y  +P   K  F           +  TGD G     D +L ++GR  +LI  N   ++I 
Sbjct: 400 YKNNPEANKAGFEF--------GWFHTGDIGYF-DTDGYLHLVGRIKELI--NRGGEKIS 459

Query: 498 PHYIEST--AYNNSSAYLRGGCIAAVKISETIAVVAEMQRDDKNDAELLRKICEEIRKAV 557
           P  +++    + + S  +  G +   K  E I      +       E ++  C++   A 
Sbjct: 460 PIEVDAVLLTHPDVSQGVAFG-VPDEKYGEEINCAVIPREGTTVTEEDIKAFCKKNLAAF 508

Query: 558 SIEEGTELGLVVLVKRGNIPKTTSGKVKRWAVKE 589
            + +   +         N+PKT SGK++R  V +
Sbjct: 520 KVPKRVFI-------TDNLPKTASGKIQRRIVAQ 508

BLAST of Bhi04G000149 vs. TAIR10
Match: AT5G38120.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 62.8 bits (151), Expect = 8.8e-10
Identity = 125/588 (21.26%), Postives = 230/588 (39.12%), Query Frame = 0

Query: 3   YENFDPL-FPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTAGSLNDGSFLTYQQLHDSV 62
           Y    PL  P +  +D  +  + S   +R K AFI +      S +D        L  +V
Sbjct: 25  YSKRKPLALPSKESLD--ITTFISSQTYRGKTAFIDAATDHRISFSD--------LWMAV 84

Query: 63  QFISDQLLRQ--LRRRDTVAVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCHH 122
             ++D LL    +RR D V VL    + +  +       G V    +P +   E      
Sbjct: 85  DRVADCLLHDVGIRRGDVVLVLSPNTISIPIVCLSVMSLGAVLTTANPLNTASE------ 144

Query: 123 LARALSQTKPRAAIAHQSYIYTIFRYLSSSPTDEKLALLLQSVQWISMDSLKQPDKKAEV 182
           + R ++ + P+ A             L+       ++++L+ V+    D+L+ P +  +V
Sbjct: 145 ILRQIADSNPKLAFTTPE--------LAPKIASSGISIVLERVE----DTLRVP-RGLKV 204

Query: 183 NQDKHQPILYHSSSYYGCN---PKEPYLIQYTSGATAIPKPVVVTAG-AAAHNVRVARKA 242
             +  + +    S     N     +  ++ Y+SG T   K V  + G   AH  R   + 
Sbjct: 205 VGNLTEMMKKEPSGQAVRNQVHKDDTAMLLYSSGTTGRSKGVNSSHGNLIAHVARYIAEP 264

Query: 243 YNLNPNDVIVSWLPQYHDCGLMFLLLSVITGATCVLTSPMSFVTNPIRWLHLITAFKATC 302
           +   P    +  +P +H  GL+  +L+ +   T V+  P   +   +  +    A     
Sbjct: 265 FE-QPQQTFICTVPLFHTFGLLNFVLATLALGTTVVILPRFDLGEMMAAVEKYRATTLIL 324

Query: 303 TPVPSFTLPLVLKRVKDETPSSKGLDLRSLRNLILINEPIYRAAVEEFVDEFKAVGLDPG 362
            P      P+++  +       K  D+  LR +     P+ +   + F+ ++  V +  G
Sbjct: 325 VP------PVLVTMINKADQIMKKYDVSFLRTVRCGGAPLSKEVTQGFMKKYPTVDVYQG 384

Query: 363 CVSPSYGLAENCTFVSTAWSGGRRWWFPAMPNYRKLLPSGRLRDGLCTEIKVVVVNGETG 422
                Y L E         S G      ++   R+    G L  G    ++  +V+  TG
Sbjct: 385 -----YALTE---------SNGAGASIESVEESRRYGAVGLLSCG----VEARIVDPNTG 444

Query: 423 EVVEDGVEGEIWVSSPSNASGYLGHPSLTKKTFHSKINNKSSPNFIRTGDRGVIRGADRF 482
           +V+     GE+W+  PS A GY          F ++    +S  +++TGD   I   D F
Sbjct: 445 QVMGLNQTGELWLKGPSIAKGY----------FRNEEEIITSEGWLKTGDLCYIDN-DGF 504

Query: 483 LFVIGRCSDLIKFNNTQQEIHPHYIESTAYNNSSAYLRGGCIAAVKISETIAVVAEMQRD 542
           LF++ R  +LIK+   Q  + P  +E+   N+             K +    +    ++ 
Sbjct: 505 LFIVDRLKELIKYKGYQ--VPPAELEALLLNHPDILDAAVIPFPDKEAGQFPMAYVARKP 539

Query: 543 DKNDAELLRKICEEIRKAVSIEEGTELGLVVLVKRGNIPKTTSGKVKR 584
           + N  E  +K+ + I K V+  +  ++  V  +   +IPKT SGK  R
Sbjct: 565 ESNLCE--KKVIDFISKQVAPYK--KIRKVAFI--DSIPKTPSGKTLR 539

BLAST of Bhi04G000149 vs. TAIR10
Match: AT3G16170.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 51.2 bits (121), Expect = 2.7e-06
Identity = 96/415 (23.13%), Postives = 163/415 (39.28%), Query Frame = 0

Query: 201 EPYLIQYTSGATAIPKPVVVTAGAAAHNVRVARKAYNLNPNDVIVSWLPQYHDCGLMFLL 260
           +P LI YTSG T  PK VV T  +    VR+  +A+     D  +  LP +H  GL   L
Sbjct: 171 DPALIVYTSGTTGKPKGVVHTHNSINSQVRMLTEAWEYTSADHFLHCLPLHHVHGLFNAL 230

Query: 261 LSVITGATCVLTSPM------------SFVTNPIRWLHLITAFKATCTPVPSFTLPLV-- 320
            + +   + V   P             S+  N  +    IT F    T VP+    L+  
Sbjct: 231 FAPLYARSLVEFLPKFSVSGIWRRWRESYPVNDEKTNDSITVF----TGVPTMYTRLIQG 290

Query: 321 LKRVKDETPSSKGLDLRSLRNLILINEPIYRAAVEEFVDEFKAVGLDPGCVSPSYGLAEN 380
            + +  E   S     R LR ++  +  + R  + ++        +    +   YG+ E 
Sbjct: 291 YEAMDKEMQDSSAFAARKLRLMMSGSSALPRPVMHQW------ESITGHRLLERYGMTEF 350

Query: 381 CTFVSTAWSGGRRWWFPAMPNYRKLLPSGRLRDGLCTEIKVVVVNGETGEVVEDGVEGEI 440
              +S    G R       P     LP          E K+     +  E   +GV GEI
Sbjct: 351 VMAMSNPLRGARNAGTVGKP-----LPG--------VEAKI-----KEDENDANGV-GEI 410

Query: 441 WVSSPSNASGYLGHPSLTKKTFHSKINNKSSPNFIRTGDRGVIRGADRFLFVIGRCS-DL 500
            V SPS    Y   P +TK++F       +   + +TGD G +   D +  ++GR S D+
Sbjct: 411 CVKSPSLFKEYWNLPEVTKESF-------TEDGYFKTGDAGRV-DEDGYYVILGRNSADI 470

Query: 501 IKFNNTQQEIHPHYIESTAYNNSSAYL----------RGGCIAAVKISETIAVVAEMQRD 560
           +K      ++    IEST   + +              G  + A+ I+E+ A   + + D
Sbjct: 471 MKVGG--YKLSALEIESTLLEHPTVAECCVLGLTDNDYGEAVTAIIIAESAA--KKRRED 530

Query: 561 DKNDAELLRKICEEIRKAVSIEEGTELGLVVLVKRGNIPKTTSGKVKRWAVKEKL 591
           +      L ++C   +  ++     +L   +L+   ++P+   GKV +  +K+ L
Sbjct: 531 ESKPVITLEELCGWAKDKLA---PYKLPTRLLIWE-SLPRNAMGKVNKKELKKSL 540

BLAST of Bhi04G000149 vs. TrEMBL
Match: tr|A0A0A0LL23|A0A0A0LL23_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G361690 PE=4 SV=1)

HSP 1 Score: 1036.9 bits (2680), Expect = 1.8e-299
Identity = 513/626 (81.95%), Postives = 565/626 (90.26%), Query Frame = 0

Query: 1   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTAGSLNDGSFLTYQQLHDS 60
           MLYENFDPLFP+QPVVDRYLPVWASLPAF+SKPAFIWSEDGTA ++N+ SFLTY+QLHDS
Sbjct: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFQSKPAFIWSEDGTAKAINEDSFLTYRQLHDS 60

Query: 61  VQFISDQLLRQLRRRDTVAVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCHHL 120
           VQ I++QLLRQLRRRDTV VLCSAGL+ VQLIYGCQRAGLVSVPISPPD F ENENCHHL
Sbjct: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHL 120

Query: 121 ARALSQTKPRAAIAHQSYIYTIFRYLSSSPTDEKLALLLQSVQWISMDSLKQPDKKAEVN 180
           ARALSQTKPRAAIAHQSYI T+FRY+SSS  D+KLALLL+ V+WISM+SLKQP K++E+N
Sbjct: 121 ARALSQTKPRAAIAHQSYINTVFRYISSS-ADKKLALLLKGVRWISMESLKQPHKESELN 180

Query: 181 QDKHQPILYHSSSYYGCNPKEPYLIQYTSGATAIPKPVVVTAGAAAHNVRVARKAYNLNP 240
           Q KHQ   YHSSSY GCNP +PYLIQYTSGATAI K VV+TAGAAAHNVR ARKAY+LNP
Sbjct: 181 QHKHQSSFYHSSSYSGCNPDDPYLIQYTSGATAISKAVVITAGAAAHNVRAARKAYDLNP 240

Query: 241 NDVIVSWLPQYHDCGLMFLLLSVITGATCVLTSPMSFVTNPIRWLHLITAFKATCTPVPS 300
           ND+IVSWLPQYHDCGLMFLLL+VITGATCVLTSP+SFVT PI WLHLITAFKATCTPVPS
Sbjct: 241 NDLIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPS 300

Query: 301 FTLPLVLKRVKDETPSSKGLDLRSLRNLILINEPIYRAAVEEFVDEFKAVGLDPGCVSPS 360
           FTLPLVLKRV   +  +  LDLRSLRNLILINEPIYR+ VEEFV+ FK VGLDPGCVSPS
Sbjct: 301 FTLPLVLKRVGYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVEVFKTVGLDPGCVSPS 360

Query: 361 YGLAENCTFVSTAWSGGRRWWFPAMPNYRKLLPSGRLRDGLCTEIKVVVVNGETGEVVED 420
           YGLAENCTFVSTAW GG    FPAMP+YR+LLPSGRLRDG+C EI+VVVVN ETGEVVED
Sbjct: 361 YGLAENCTFVSTAWCGG--GGFPAMPSYRQLLPSGRLRDGMCKEIEVVVVNEETGEVVED 420

Query: 421 GVEGEIWVSSPSNASGYLGHPSLTKKTFHSKINNKSSPNFIRTGDRGVIRGADRFLFVIG 480
           GVEGEIWVSSPSNA GYLGHPS+T++TFHSK+ NKSS NF+RTGDRGVI+G+DRFLFVIG
Sbjct: 421 GVEGEIWVSSPSNALGYLGHPSVTEETFHSKLKNKSSLNFVRTGDRGVIKGSDRFLFVIG 480

Query: 481 RCSDLIKFNNTQQEIHPHYIESTAYNNSSAYLRGGCIAAVKISETIAVVAEMQRDDKNDA 540
           RCSD+IK NN  Q+IHPHYIEST+YNN SAYLRGGCIAAVK+S TIA+VAEMQRDD++DA
Sbjct: 481 RCSDVIKLNNNNQQIHPHYIESTSYNNFSAYLRGGCIAAVKVSGTIALVAEMQRDDRHDA 540

Query: 541 ELLRKICEEIRKAVSIEEGTELGLVVLVKRGNIPKTTSGKVKRWAVKEKLAGGGLGVLMA 600
           ELLRKICE+IRKAV IEEG ELGLVVLVKRGN+ KTTSGKVKRW VKEKLAGGGLGVLMA
Sbjct: 541 ELLRKICEDIRKAVLIEEGIELGLVVLVKRGNVSKTTSGKVKRWVVKEKLAGGGLGVLMA 600

Query: 601 IGFEKNCEVLQREEELRTRPALLSLL 627
             F KNC  L+R+ E  TRP L+S+L
Sbjct: 601 FEFGKNCVDLKRKGEFETRPVLISML 623

BLAST of Bhi04G000149 vs. TrEMBL
Match: tr|A0A1S3BAI6|A0A1S3BAI6_CUCME (long-chain-fatty-acid--AMP ligase FadD26-like OS=Cucumis melo OX=3656 GN=LOC103487973 PE=4 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 1.3e-209
Identity = 371/465 (79.78%), Postives = 411/465 (88.39%), Query Frame = 0

Query: 167 MDSLKQPDKKAEVNQDKHQPILYHSSSYYGCNPKEPYLIQYTSGATAIPKPVVVTAGAAA 226
           M+SLK+P K++E+NQ KH    +HSSSY GCNP +PYLIQYTSGATAI KPVV+TAGAAA
Sbjct: 1   MESLKEPHKESELNQHKHHSFSHHSSSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAA 60

Query: 227 HNVRVARKAYNLNPNDVIVSWLPQYHDCGLMFLLLSVITGATCVLTSPMSFVTNPIRWLH 286
           HNVR ARKAY+LNPNDVIVSWLPQ+HDCGLMFLLL+VITGATCVLTSP+SFVT PI WLH
Sbjct: 61  HNVRAARKAYDLNPNDVIVSWLPQFHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLH 120

Query: 287 LITAFKATCTPVPSFTLPLVLKRVKDETPSSKG-LDLRSLRNLILINEPIYRAAVEEFVD 346
           LITAFKATCTPVPSFTLPLVLKRV + +    G LDLRSLRNLILINEPIYR+ VEEFVD
Sbjct: 121 LITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVD 180

Query: 347 EFKAVGLDPGCVSPSYGLAENCTFVSTAWSGGRRWWFPAMPNYRKLLPSGRLRDGLCTEI 406
            FKAVGLDPGCVSPSYGLAENCTFVSTAW GG    FPAMP+YRKLLPSGRLRDG+C EI
Sbjct: 181 VFKAVGLDPGCVSPSYGLAENCTFVSTAWCGG--GGFPAMPSYRKLLPSGRLRDGMCKEI 240

Query: 407 KVVVVNGETGEVVEDGVEGEIWVSSPSNASGYLGHPSLTKKTFHSKINNKSSPNFIRTGD 466
           +VVVVN ETGEVVEDGVEGEIW+SSPSNA GYLGHPSLT++TFH K+ NK+S NF+RTGD
Sbjct: 241 EVVVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPSLTEETFHRKLKNKASLNFVRTGD 300

Query: 467 RGVIRGADRFLFVIGRCSDLIKFNNTQQEIHPHYIESTAYNNSSAYLRGGCIAAVKISE- 526
           RGVI+G+DRFLFVIGRCSD+IKFN+  Q+IHPHYIEST+Y N SAYLRGGCIAAVKIS  
Sbjct: 301 RGVIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHYIESTSYKNFSAYLRGGCIAAVKISSG 360

Query: 527 TIAVVAEMQRDDKNDAELLRKICEEIRKAVSIEEGTELGLVVLVKRGNIPKTTSGKVKRW 586
           T+A+VAEMQRDD+NDAELLRKICEEIRKAV IEEG ELG+VVLVKRGN+ KTTSGKVKRW
Sbjct: 361 TVALVAEMQRDDRNDAELLRKICEEIRKAVLIEEGIELGVVVLVKRGNVSKTTSGKVKRW 420

Query: 587 AVKEKLAGGGLGVLMAIGFEKNCE---VLQREEELRTRPALLSLL 627
            VKEKL GGGL VLMA+ F K+CE    L+R+ E  TRP LLS+L
Sbjct: 421 VVKEKLGGGGLEVLMAVEFGKDCEGLKDLERKGEFGTRPVLLSML 463

BLAST of Bhi04G000149 vs. TrEMBL
Match: tr|A0A0D2S4U7|A0A0D2S4U7_GOSRA (Uncharacterized protein OS=Gossypium raimondii OX=29730 GN=B456_009G201400 PE=4 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 6.1e-207
Identity = 370/610 (60.66%), Postives = 463/610 (75.90%), Query Frame = 0

Query: 1   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTAGSLNDGSFLTYQQLHDS 60
           M YEN+DP+FPDQPVVD+YLP+WASL AFRSKPAFIWS+DGT  ++++GS LTY QL+DS
Sbjct: 1   MNYENYDPIFPDQPVVDQYLPIWASLSAFRSKPAFIWSDDGTT-NVSNGSTLTYAQLNDS 60

Query: 61  VQFISDQLLRQLRRRDTVAVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCHHL 120
           VQ IS QLL  L+R DTV +LC+ GLELV++I+GCQRAGL+SVPI PPDP    +N HHL
Sbjct: 61  VQSISIQLLHSLQRGDTVIILCTPGLELVEIIFGCQRAGLLSVPIFPPDPSFSKQNHHHL 120

Query: 121 ARALSQTKPRAAIAHQSYIYTIFRYLSSSPTDEKLALLLQSVQWISMDSLKQPDKKAEVN 180
            RALSQTKP+AAIAH  YI  + +YLS    D+KLA +LQ+++W+  + +K   K A+  
Sbjct: 121 LRALSQTKPKAAIAHHDYITKVQQYLSLRSKDKKLAQMLQNLKWVPTEDVKH--KNADSR 180

Query: 181 QDKHQPILYHSSSYYGCNPKEPYLIQYTSGATAIPKPVVVTAGAAAHNVRVARKAYNLNP 240
            D        S SY GC P E YLIQYTSGAT IPKPV+VTAG+AAHNVR ARKAY+L+P
Sbjct: 181 LD--------SMSYDGCKPDETYLIQYTSGATGIPKPVLVTAGSAAHNVRTARKAYDLHP 240

Query: 241 NDVIVSWLPQYHDCGLMFLLLSVITGATCVLTSPMSFVTNPIRWLHLITAFKATCTPVPS 300
           N+VIVSWLPQYHDCGLMFLLL++++GATCVLTSP +FV  P  W+ LIT FKATCTPVPS
Sbjct: 241 NNVIVSWLPQYHDCGLMFLLLTIVSGATCVLTSPAAFVNRPRLWIELITQFKATCTPVPS 300

Query: 301 FTLPLVLKRVKDETPSSKGLDLRSLRNLILINEPIYRAAVEEFVDEFKAVGLDPGCVSPS 360
           FTLPLV+KR   ET SS  ++L SLRNLI+INEPIY+A+VEEF+  FK  GL+P  +SPS
Sbjct: 301 FTLPLVVKRGGVETGSSP-INLWSLRNLIIINEPIYKASVEEFLHVFKPFGLNPSSISPS 360

Query: 361 YGLAENCTFVSTAW---SGGRRWWFPAMPNYRKLLPSGRLRDGLCTE-IKVVVVNGETGE 420
           YGLAENCTFVSTAW          FP  P++ KLLP  RL      E + ++VVN  T E
Sbjct: 361 YGLAENCTFVSTAWRNDDNSGNSCFPVFPSHNKLLPIARLASEEEEEDMDIIVVNEHTHE 420

Query: 421 VVEDGVEGEIWVSSPSNASGYLGHPSLTKKTFHSKINNKSSPNFIRTGDRGVIRGADRFL 480
            V+DG+EGEIWVSSPSNASGYLGHP LT+  F  +++NK S  F+RTGD+G+++G +RFL
Sbjct: 421 PVDDGIEGEIWVSSPSNASGYLGHPFLTQDIFKGRLSNKVSRCFVRTGDKGIVKGEERFL 480

Query: 481 FVIGRCSDLIKFNNTQQEIHPHYIESTAYNNSSAYLRGGCIAAVKISETIAVVAEMQRDD 540
           FV GRC+++IK  N  Q+IHPHYIE+ AYN+   ++RGGC+AA  +S  I +VAEMQR +
Sbjct: 481 FVTGRCAEVIKLPN-GQDIHPHYIETAAYNSCPQFIRGGCLAAFDVSRMIVLVAEMQRSE 540

Query: 541 KNDAELLRKICEEIRKAVSIEEGTELGLVVLVKRGNIPKTTSGKVKRWAVKEKLAGGGLG 600
           K D ++LR ICE IR+ V   E  ++G+VVLVK G++PKTTSGK++RWA K+   GG + 
Sbjct: 541 K-DNKILRNICERIRENVFENEKVDVGMVVLVKSGSVPKTTSGKIQRWAAKQYFLGGKMN 596

Query: 601 VLMAIGFEKN 607
            LM + F+ N
Sbjct: 601 ALMELKFDNN 596

BLAST of Bhi04G000149 vs. TrEMBL
Match: tr|A0A2P5RFD4|A0A2P5RFD4_GOSBA (Uncharacterized protein OS=Gossypium barbadense OX=3634 GN=GOBAR_DD17554 PE=4 SV=1)

HSP 1 Score: 729.2 bits (1881), Expect = 8.0e-207
Identity = 371/610 (60.82%), Postives = 463/610 (75.90%), Query Frame = 0

Query: 1   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTAGSLNDGSFLTYQQLHDS 60
           M YEN+DP+FPDQPVVD+YLP+WASL AFRSKPAFIWSEDGT  ++++GS LTY QL+DS
Sbjct: 1   MNYENYDPIFPDQPVVDQYLPIWASLSAFRSKPAFIWSEDGTT-NVSNGSTLTYAQLNDS 60

Query: 61  VQFISDQLLRQLRRRDTVAVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCHHL 120
           VQ IS QLL  L+R DTV +LC+ GLELV++I+GCQRAGL+SVPI PPDP    +N HHL
Sbjct: 61  VQSISIQLLHSLQRGDTVIILCTPGLELVEIIFGCQRAGLLSVPIFPPDPSFSKQNHHHL 120

Query: 121 ARALSQTKPRAAIAHQSYIYTIFRYLSSSPTDEKLALLLQSVQWISMDSLKQPDKKAEVN 180
            RALSQTKP+AAIAH  YI  + +YLS    D+KLA +LQ+++W+  + +K   K A+  
Sbjct: 121 LRALSQTKPKAAIAHHDYITKVQQYLSLRSKDKKLAQMLQNLKWVPTEDVKH--KNADSR 180

Query: 181 QDKHQPILYHSSSYYGCNPKEPYLIQYTSGATAIPKPVVVTAGAAAHNVRVARKAYNLNP 240
            D        S SY GC P E YLIQYTSGAT IPKPV+VTAG+AAHNVR ARKAY+L+P
Sbjct: 181 LD--------SMSYDGCKPDETYLIQYTSGATGIPKPVLVTAGSAAHNVRTARKAYDLHP 240

Query: 241 NDVIVSWLPQYHDCGLMFLLLSVITGATCVLTSPMSFVTNPIRWLHLITAFKATCTPVPS 300
           N+VIVSWLPQYHDCGLMFLLL++++GATCVLTS  +FV  P  W+ LIT FKATCTPVPS
Sbjct: 241 NNVIVSWLPQYHDCGLMFLLLTIVSGATCVLTSAAAFVNRPRLWIELITQFKATCTPVPS 300

Query: 301 FTLPLVLKRVKDETPSSKGLDLRSLRNLILINEPIYRAAVEEFVDEFKAVGLDPGCVSPS 360
           FTLPLV+KR   ET SS  ++L SLRNLI+INEPIY+A+VEEF+  FK  GL+P  +SPS
Sbjct: 301 FTLPLVVKRGGVETGSSP-INLWSLRNLIIINEPIYKASVEEFLHVFKPFGLNPSSISPS 360

Query: 361 YGLAENCTFVSTAW---SGGRRWWFPAMPNYRKLLPSGRLRDGLCTE-IKVVVVNGETGE 420
           YGLAENCTFVSTAW          FP  P++ KLLP  RL      E + ++VVN  T E
Sbjct: 361 YGLAENCTFVSTAWRNDDNSGNSCFPVFPSHNKLLPIARLASEEEEEDMDIIVVNEHTHE 420

Query: 421 VVEDGVEGEIWVSSPSNASGYLGHPSLTKKTFHSKINNKSSPNFIRTGDRGVIRGADRFL 480
            V+DG+EGEIWVSSPSNASGYLGHP LT+  F  +++NK S  F+RTGD+G+++G +RFL
Sbjct: 421 PVDDGIEGEIWVSSPSNASGYLGHPFLTQDIFKGRLSNKVSRCFVRTGDKGIVKGEERFL 480

Query: 481 FVIGRCSDLIKFNNTQQEIHPHYIESTAYNNSSAYLRGGCIAAVKISETIAVVAEMQRDD 540
           FV GRC+++IK  N  Q+IHPHYIE+ AYN+   ++RGGC+AA  +S  IA+VAEMQR +
Sbjct: 481 FVTGRCAEVIKLPN-GQDIHPHYIETAAYNSCPQFIRGGCLAAFDVSRMIALVAEMQRSE 540

Query: 541 KNDAELLRKICEEIRKAVSIEEGTELGLVVLVKRGNIPKTTSGKVKRWAVKEKLAGGGLG 600
           K D ++LR ICE IR+ V   E  ++G+VVLVK G++PKTTSGK++RWA K+   GG + 
Sbjct: 541 K-DNKILRNICERIRENVFENEKVDVGMVVLVKSGSVPKTTSGKIQRWAAKQYFLGGKMN 596

Query: 601 VLMAIGFEKN 607
            LM + F+ N
Sbjct: 601 ALMELKFDNN 596

BLAST of Bhi04G000149 vs. TrEMBL
Match: tr|A0A2C9VWM7|A0A2C9VWM7_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_05G158600 PE=4 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 1.4e-206
Identity = 371/607 (61.12%), Postives = 460/607 (75.78%), Query Frame = 0

Query: 1   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTAGSLNDGSFLTYQQLHDS 60
           M YEN+DP FPDQPVVD YLPVWA LP+F SKPAFIW+EDG++G+    S LTY QL+DS
Sbjct: 1   MNYENYDPSFPDQPVVDLYLPVWARLPSFSSKPAFIWAEDGSSGA-TKASVLTYSQLNDS 60

Query: 61  VQFISDQLLRQLRRRDTVAVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCHHL 120
            Q IS+QL   L R +TV VL   GLELV++I+GCQRAGL+SVPI PPDP   N N HHL
Sbjct: 61  TQLISNQLHELLPRGNTVIVLSPPGLELVEVIFGCQRAGLLSVPIFPPDPSFANGNYHHL 120

Query: 121 ARALSQTKPRAAIAHQSYIYTIFRYLSSSPTDEKLALLLQSVQWISMDSLKQPDKKAEVN 180
            R LSQTKP+ AIA Q YI ++ RYLSSS +++KL+ LLQ + WIS   LK   KK  ++
Sbjct: 121 VRVLSQTKPKVAIADQDYIASVQRYLSSSSSNQKLSELLQKLIWISTCDLK--GKKVGLS 180

Query: 181 QDKHQPILYHSSSYYGCNPKEPYLIQYTSGATAIPKPVVVTAGAAAHNVRVARKAYNLNP 240
            D        SSSY GC P E YLIQYTSGAT IPKPV+VTAG+A HNVRVARKAY+L+P
Sbjct: 181 MD--------SSSYNGCRPNEVYLIQYTSGATGIPKPVLVTAGSATHNVRVARKAYDLHP 240

Query: 241 NDVIVSWLPQYHDCGLMFLLLSVITGATCVLTSPMSFVTNPIRWLHLITAFKATCTPVPS 300
           N VIVSWLPQYHDCGL FLLL+V++GATCVLTSP  F+T P  WL LI+ FKATCTPVPS
Sbjct: 241 NSVIVSWLPQYHDCGLQFLLLTVVSGATCVLTSPGEFITRPRLWLELISEFKATCTPVPS 300

Query: 301 FTLPLVLKRVKDETPSSKGLDLRSLRNLILINEPIYRAAVEEFVDEFKAVGLDPGCVSPS 360
           FTLPLV+KR   E   ++ + L SL+NLI+INEPIY+A VEEF+D FK  GLDP C+SPS
Sbjct: 301 FTLPLVVKRGGVE-QGTRPVTLWSLKNLIIINEPIYKAPVEEFIDVFKPFGLDPSCISPS 360

Query: 361 YGLAENCTFVSTAWSGGRRWWFPAMPNYRKLLPSGRL---RDGLCTEIKVVVVNGETGEV 420
           YGLAENCTFVSTAW       F + P+Y KLLPS RL    +    ++ ++VVN +T E 
Sbjct: 361 YGLAENCTFVSTAWRCNGN--FGSFPSYNKLLPSARLSCQHEQDDEDMDIIVVNEDTHET 420

Query: 421 VEDGVEGEIWVSSPSNASGYLGHPSLTKKTFHSKINNKSSPNFIRTGDRGVIRGADRFLF 480
           V DG+EGEIW+SSPSN SGYLGHPSLT++ F +++ NK S  F+RTGDRG++ G +RFL+
Sbjct: 421 VPDGIEGEIWISSPSNCSGYLGHPSLTREIFQARLRNKVSRCFVRTGDRGIVGGEERFLY 480

Query: 481 VIGRCSDLIKFNNTQQEIHPHYIESTAYNNSSAYLRGGCIAAVKISETIAVVAEMQRDDK 540
           + GRCSD+I+  N Q++ HPHYIE+ AYN    +LRGGC+AA++IS+ +A+VAE QR + 
Sbjct: 481 ITGRCSDVIRLRNGQEK-HPHYIETAAYNCYPDFLRGGCLAAIQISDKVALVAETQRRE- 540

Query: 541 NDAELLRKICEEIRKAVSIEEGTELGLVVLVKRGNIPKTTSGKVKRWAVKEKLAGGGLGV 600
           N+  +LR+ICE I+K V  EE  E+GL+VLVK GN+PKTTSGK++RWA KEKL GG + +
Sbjct: 541 NNVSVLRQICEGIKKGVMDEERVEIGLIVLVKSGNLPKTTSGKIQRWATKEKLVGGKMSI 591

Query: 601 LMAIGFE 605
           +M + F+
Sbjct: 601 IMEMLFD 591

BLAST of Bhi04G000149 vs. NCBI nr
Match: XP_011649625.1 (PREDICTED: disco-interacting protein 2 homolog C-like [Cucumis sativus] >KGN62605.1 hypothetical protein Csa_2G361690 [Cucumis sativus])

HSP 1 Score: 1036.9 bits (2680), Expect = 2.7e-299
Identity = 513/626 (81.95%), Postives = 565/626 (90.26%), Query Frame = 0

Query: 1   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTAGSLNDGSFLTYQQLHDS 60
           MLYENFDPLFP+QPVVDRYLPVWASLPAF+SKPAFIWSEDGTA ++N+ SFLTY+QLHDS
Sbjct: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFQSKPAFIWSEDGTAKAINEDSFLTYRQLHDS 60

Query: 61  VQFISDQLLRQLRRRDTVAVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCHHL 120
           VQ I++QLLRQLRRRDTV VLCSAGL+ VQLIYGCQRAGLVSVPISPPD F ENENCHHL
Sbjct: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHL 120

Query: 121 ARALSQTKPRAAIAHQSYIYTIFRYLSSSPTDEKLALLLQSVQWISMDSLKQPDKKAEVN 180
           ARALSQTKPRAAIAHQSYI T+FRY+SSS  D+KLALLL+ V+WISM+SLKQP K++E+N
Sbjct: 121 ARALSQTKPRAAIAHQSYINTVFRYISSS-ADKKLALLLKGVRWISMESLKQPHKESELN 180

Query: 181 QDKHQPILYHSSSYYGCNPKEPYLIQYTSGATAIPKPVVVTAGAAAHNVRVARKAYNLNP 240
           Q KHQ   YHSSSY GCNP +PYLIQYTSGATAI K VV+TAGAAAHNVR ARKAY+LNP
Sbjct: 181 QHKHQSSFYHSSSYSGCNPDDPYLIQYTSGATAISKAVVITAGAAAHNVRAARKAYDLNP 240

Query: 241 NDVIVSWLPQYHDCGLMFLLLSVITGATCVLTSPMSFVTNPIRWLHLITAFKATCTPVPS 300
           ND+IVSWLPQYHDCGLMFLLL+VITGATCVLTSP+SFVT PI WLHLITAFKATCTPVPS
Sbjct: 241 NDLIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPS 300

Query: 301 FTLPLVLKRVKDETPSSKGLDLRSLRNLILINEPIYRAAVEEFVDEFKAVGLDPGCVSPS 360
           FTLPLVLKRV   +  +  LDLRSLRNLILINEPIYR+ VEEFV+ FK VGLDPGCVSPS
Sbjct: 301 FTLPLVLKRVGYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVEVFKTVGLDPGCVSPS 360

Query: 361 YGLAENCTFVSTAWSGGRRWWFPAMPNYRKLLPSGRLRDGLCTEIKVVVVNGETGEVVED 420
           YGLAENCTFVSTAW GG    FPAMP+YR+LLPSGRLRDG+C EI+VVVVN ETGEVVED
Sbjct: 361 YGLAENCTFVSTAWCGG--GGFPAMPSYRQLLPSGRLRDGMCKEIEVVVVNEETGEVVED 420

Query: 421 GVEGEIWVSSPSNASGYLGHPSLTKKTFHSKINNKSSPNFIRTGDRGVIRGADRFLFVIG 480
           GVEGEIWVSSPSNA GYLGHPS+T++TFHSK+ NKSS NF+RTGDRGVI+G+DRFLFVIG
Sbjct: 421 GVEGEIWVSSPSNALGYLGHPSVTEETFHSKLKNKSSLNFVRTGDRGVIKGSDRFLFVIG 480

Query: 481 RCSDLIKFNNTQQEIHPHYIESTAYNNSSAYLRGGCIAAVKISETIAVVAEMQRDDKNDA 540
           RCSD+IK NN  Q+IHPHYIEST+YNN SAYLRGGCIAAVK+S TIA+VAEMQRDD++DA
Sbjct: 481 RCSDVIKLNNNNQQIHPHYIESTSYNNFSAYLRGGCIAAVKVSGTIALVAEMQRDDRHDA 540

Query: 541 ELLRKICEEIRKAVSIEEGTELGLVVLVKRGNIPKTTSGKVKRWAVKEKLAGGGLGVLMA 600
           ELLRKICE+IRKAV IEEG ELGLVVLVKRGN+ KTTSGKVKRW VKEKLAGGGLGVLMA
Sbjct: 541 ELLRKICEDIRKAVLIEEGIELGLVVLVKRGNVSKTTSGKVKRWVVKEKLAGGGLGVLMA 600

Query: 601 IGFEKNCEVLQREEELRTRPALLSLL 627
             F KNC  L+R+ E  TRP L+S+L
Sbjct: 601 FEFGKNCVDLKRKGEFETRPVLISML 623

BLAST of Bhi04G000149 vs. NCBI nr
Match: XP_022962563.1 (uncharacterized protein LOC111462961 [Cucurbita moschata])

HSP 1 Score: 1018.1 bits (2631), Expect = 1.3e-293
Identity = 510/635 (80.31%), Postives = 554/635 (87.24%), Query Frame = 0

Query: 1   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTAGSLNDGSFLTYQQLHDS 60
           M YENFDP+FPDQPVVD YLPVWASLPAFRSKPAFIWSEDGTA  LN+GSFLTY QLHDS
Sbjct: 14  MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 73

Query: 61  VQFISDQLLRQLRRRDTVAVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCHHL 120
           VQFISD+L+RQ+RRRDTV +LCS GL+LVQLIYGCQRAGLVSVPISPPDP  ENENCHHL
Sbjct: 74  VQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENCHHL 133

Query: 121 ARALSQTKPRAAIAHQSYIYTIFRYLSSSPTDEKLALLLQSVQWISMDSLK-----QPDK 180
           ARALSQTKPRAAIAHQ+YI ++FRYLS SPTD KLALLLQSVQWISM++L+     + D+
Sbjct: 134 ARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLQSVQWISMETLRTCTAAEADQ 193

Query: 181 KAEVNQDKHQPILYHS-SSYYGCNPKEPYLIQYTSGATAIPKPVVVTAGAAAHNVRVARK 240
              V   K QP  YHS SSYYGC P+EPYLIQYTSGAT IPKPVVVTAGAAAHNVR ARK
Sbjct: 194 VVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAARK 253

Query: 241 AYNLNPNDVIVSWLPQYHDCGLMFLLLSVITGATCVLTSPMSFVTNPIRWLHLITAFKAT 300
           AY+LNPNDVIVSWLPQYHDCGLMFLLL+V++GATCVLTSP+SFVT PI WLHLIT FKAT
Sbjct: 254 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 313

Query: 301 CTPVPSFTLPLVLKRVKDETPSSKGLDLRSLRNLILINEPIYRAAVEEFVDEFKAVGLDP 360
           CTPVPSFTLPLVLKRVK+ETP   GLDL SLRNLILINEP+YR+ VEEFVD FKAVGL+P
Sbjct: 314 CTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAVGLNP 373

Query: 361 GCVSPSYGLAENCTFVSTAWS---GGRRWWFPAMPNYRKLLPSGRLRDGLCTEIKVVVVN 420
           GCVSPSYGLAENCTFVSTAWS   GGRR WFPAMP+YRKLLPS RL D   +EI+VVVVN
Sbjct: 374 GCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPSARLMDESSSEIEVVVVN 433

Query: 421 GETGEVVEDGVEGEIWVSSPSNASGYLGHPSLTKKTFHSKINNKSSPNFIRTGDRGVIRG 480
           GETGE+VEDGVEGEIW+SSPSNASGYLGHPSLT+ TFH K++NKSS  F+RTGDRGVI+G
Sbjct: 434 GETGEIVEDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKLSNKSSHKFVRTGDRGVIKG 493

Query: 481 ADRFLFVIGRCSDLIKFNNTQQEIHPHYIESTAYNNSSAYLRGGCIAAVKISETIAVVAE 540
            DRFLFVIGRCSD+I   N  QEIHPHYIES AYNN S+YLRGGC+AA+KIS+TIAVVAE
Sbjct: 494 TDRFLFVIGRCSDVITLTN-NQEIHPHYIESIAYNNCSSYLRGGCLAAIKISDTIAVVAE 553

Query: 541 MQRDDKNDAELLRKICEEIRKAVSIEEGTELGLVVLVKRGNIPKTTSGKVKRWAVKEKLA 600
           MQR DKNDAE LRKICE IRKA  IEEG ELGLVVLVKRGN+PKTTSGKVKRWA KEKLA
Sbjct: 554 MQRQDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKEKLA 613

Query: 601 GGGLGVLMAIGFEKNCEVLQREEELRTRPALLSLL 627
           GGG+ VLMA+ F K C V +  E    RP LLSLL
Sbjct: 614 GGGMSVLMAVKFGKTCGVTKDLE--TNRPLLLSLL 645

BLAST of Bhi04G000149 vs. NCBI nr
Match: XP_023546350.1 (uncharacterized protein LOC111805474 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1013.8 bits (2620), Expect = 2.5e-292
Identity = 508/635 (80.00%), Postives = 553/635 (87.09%), Query Frame = 0

Query: 1   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTAGSLNDGSFLTYQQLHDS 60
           M YENFDP+FPDQPVVD YLPVWASLPAFRSKPAFIWSEDGTA  LN+GSFLTY QLHDS
Sbjct: 14  MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 73

Query: 61  VQFISDQLLRQLRRRDTVAVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCHHL 120
           VQFIS+QL+RQ+RRRDTV VLCS GL+LVQLIYGCQRAGLVSVPISPPDP  EN+NCHHL
Sbjct: 74  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENKNCHHL 133

Query: 121 ARALSQTKPRAAIAHQSYIYTIFRYLSSSPTDEKLALLLQSVQWISMDSLK-----QPDK 180
           ARALSQTKPRAAIAHQ+YI ++FRYLS SPTD KLALLL+ VQWISM++L+     + D+
Sbjct: 134 ARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLRGVQWISMETLRTCTAAEADQ 193

Query: 181 KAEVNQDKHQPILYHS-SSYYGCNPKEPYLIQYTSGATAIPKPVVVTAGAAAHNVRVARK 240
              V   K QP  YHS SSYYGC P+EPYLIQYTSGAT IPKPVVVTAGAAAHNVRVARK
Sbjct: 194 VVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRVARK 253

Query: 241 AYNLNPNDVIVSWLPQYHDCGLMFLLLSVITGATCVLTSPMSFVTNPIRWLHLITAFKAT 300
           AY+LNPNDVIVSWLPQYHDCGLMFLLL+V++GATCVLTSP+SFVT PI WLHLIT FKAT
Sbjct: 254 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 313

Query: 301 CTPVPSFTLPLVLKRVKDETPSSKGLDLRSLRNLILINEPIYRAAVEEFVDEFKAVGLDP 360
           CTPVPSFTLPLVLKRVK+ETP   GLDL SLRNLILINEP+YR+ VEEFVD FK VGL+P
Sbjct: 314 CTPVPSFTLPLVLKRVKEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKTVGLNP 373

Query: 361 GCVSPSYGLAENCTFVSTAWS---GGRRWWFPAMPNYRKLLPSGRLRDGLCTEIKVVVVN 420
           GCVSPSYGLAENCTFVSTAWS   GGRR WFPAMP+YRKLLP  +L D    EI+VVVVN
Sbjct: 374 GCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPCAKLTDESSAEIEVVVVN 433

Query: 421 GETGEVVEDGVEGEIWVSSPSNASGYLGHPSLTKKTFHSKINNKSSPNFIRTGDRGVIRG 480
           GETGE+V+DGVEGEIW+SSPSNASGYLGHPSLT+ TFH K++NKSS  F+RTGDRGVI+G
Sbjct: 434 GETGEIVDDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKLSNKSSHKFVRTGDRGVIKG 493

Query: 481 ADRFLFVIGRCSDLIKFNNTQQEIHPHYIESTAYNNSSAYLRGGCIAAVKISETIAVVAE 540
            DRFLFVIGRCSD+IK NN  QEIHPHYIES AYNN SAYLRGGC+AA+KIS+TIAVVAE
Sbjct: 494 TDRFLFVIGRCSDVIKLNN-NQEIHPHYIESIAYNNFSAYLRGGCLAAIKISDTIAVVAE 553

Query: 541 MQRDDKNDAELLRKICEEIRKAVSIEEGTELGLVVLVKRGNIPKTTSGKVKRWAVKEKLA 600
           MQRDDKNDAE LRKICE IRKA  IEEG ELGLVVLVKRGN+PKTTSGKVKRWA KEKLA
Sbjct: 554 MQRDDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKEKLA 613

Query: 601 GGGLGVLMAIGFEKNCEVLQREEELRTRPALLSLL 627
           GGG+ VLMA+ F   C V +  E    RP LLSLL
Sbjct: 614 GGGMSVLMAVKFGTTCGVTKDLE--GNRPLLLSLL 645

BLAST of Bhi04G000149 vs. NCBI nr
Match: XP_022997536.1 (uncharacterized protein LOC111492429 [Cucurbita maxima])

HSP 1 Score: 984.9 bits (2545), Expect = 1.2e-283
Identity = 496/635 (78.11%), Postives = 543/635 (85.51%), Query Frame = 0

Query: 1   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTAGSLNDGSFLTYQQLHDS 60
           M YENFDP+FPDQPVVD YLPVWASLPAFRSKPAFIWSEDGTA  LN+GSFLTY QLHDS
Sbjct: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60

Query: 61  VQFISDQLLRQLRRRDTVAVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCHHL 120
           VQFIS+QL+RQ+RRRDTV VLCS GL+LVQLIYGCQRAGLVSVPISPPDP  ENENCHH 
Sbjct: 61  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENENCHHF 120

Query: 121 ARALSQTKPRAAIAHQSYIYTIFRYLSSSPTDEKLALLLQSVQWISMDSLK-----QPDK 180
           ARALSQTKPRA IAH+ YI ++FRYLSSS  D KLALLLQSVQWISM++L+     + D+
Sbjct: 121 ARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISMETLRTCTAAEADQ 180

Query: 181 KAEVNQDKHQPILYHS-SSYYGCNPKEPYLIQYTSGATAIPKPVVVTAGAAAHNVRVARK 240
              V   K +P  YHS SSYYGC P+EPYLIQYTSGAT IPKPVVVTAGAAAHNVR ARK
Sbjct: 181 VVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAARK 240

Query: 241 AYNLNPNDVIVSWLPQYHDCGLMFLLLSVITGATCVLTSPMSFVTNPIRWLHLITAFKAT 300
           AY+LNPNDVIVSWLPQYHDCGLMFLLL+V++GATCVLTSP+SFVT PI WLHLIT FKAT
Sbjct: 241 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 300

Query: 301 CTPVPSFTLPLVLKRVKDETPSSKGLDLRSLRNLILINEPIYRAAVEEFVDEFKAVGLDP 360
           CTPVPSFTLPLVLKRVK+ETP    LDL  LRNLILINEP+YR+ VEEFVD FKAVGL+P
Sbjct: 301 CTPVPSFTLPLVLKRVKEETPPGGDLDLCCLRNLILINEPVYRSGVEEFVDVFKAVGLNP 360

Query: 361 GCVSPSYGLAENCTFVSTAWSGGR---RWWFPAMPNYRKLLPSGRLRDGLCTEIKVVVVN 420
           GCVSPSYGLAEN TFVSTAWSGG    R  FPAMP+YRKLLP  RL DG   EI+VVVVN
Sbjct: 361 GCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCARLTDGSSAEIEVVVVN 420

Query: 421 GETGEVVEDGVEGEIWVSSPSNASGYLGHPSLTKKTFHSKINNKSSPNFIRTGDRGVIRG 480
           GETGE+V+DGVEGEIW+SSPSNASGYLGHP LT+ TFH K++NKSS  F+RTGDRGVI+G
Sbjct: 421 GETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKLSNKSSHKFVRTGDRGVIKG 480

Query: 481 ADRFLFVIGRCSDLIKFNNTQQEIHPHYIESTAYNNSSAYLRGGCIAAVKISETIAVVAE 540
           ADRFLFVIGRCSD+I   N  QEIHPHYIES AYNN SAYLRGGC+AA+K+S+TIAVVAE
Sbjct: 481 ADRFLFVIGRCSDVITLTN-NQEIHPHYIESIAYNNFSAYLRGGCLAAIKVSDTIAVVAE 540

Query: 541 MQRDDKNDAELLRKICEEIRKAVSIEEGTELGLVVLVKRGNIPKTTSGKVKRWAVKEKLA 600
           MQR+DKNDAE LR+ICE IRKA  IEEG ELGLVVLVKRGN+PKTTSGKVKRW  KEKLA
Sbjct: 541 MQREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWLAKEKLA 600

Query: 601 GGGLGVLMAIGFEKNCEVLQREEELRTRPALLSLL 627
           GGG+ VLMA+ F   C V +  +    RP LLSLL
Sbjct: 601 GGGMSVLMAVKFGTICGVTKDLD--GNRPLLLSLL 632

BLAST of Bhi04G000149 vs. NCBI nr
Match: XP_008444721.2 (PREDICTED: long-chain-fatty-acid--AMP ligase FadD26-like [Cucumis melo])

HSP 1 Score: 738.4 bits (1905), Expect = 2.0e-209
Identity = 371/465 (79.78%), Postives = 411/465 (88.39%), Query Frame = 0

Query: 167 MDSLKQPDKKAEVNQDKHQPILYHSSSYYGCNPKEPYLIQYTSGATAIPKPVVVTAGAAA 226
           M+SLK+P K++E+NQ KH    +HSSSY GCNP +PYLIQYTSGATAI KPVV+TAGAAA
Sbjct: 1   MESLKEPHKESELNQHKHHSFSHHSSSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAA 60

Query: 227 HNVRVARKAYNLNPNDVIVSWLPQYHDCGLMFLLLSVITGATCVLTSPMSFVTNPIRWLH 286
           HNVR ARKAY+LNPNDVIVSWLPQ+HDCGLMFLLL+VITGATCVLTSP+SFVT PI WLH
Sbjct: 61  HNVRAARKAYDLNPNDVIVSWLPQFHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLH 120

Query: 287 LITAFKATCTPVPSFTLPLVLKRVKDETPSSKG-LDLRSLRNLILINEPIYRAAVEEFVD 346
           LITAFKATCTPVPSFTLPLVLKRV + +    G LDLRSLRNLILINEPIYR+ VEEFVD
Sbjct: 121 LITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVD 180

Query: 347 EFKAVGLDPGCVSPSYGLAENCTFVSTAWSGGRRWWFPAMPNYRKLLPSGRLRDGLCTEI 406
            FKAVGLDPGCVSPSYGLAENCTFVSTAW GG    FPAMP+YRKLLPSGRLRDG+C EI
Sbjct: 181 VFKAVGLDPGCVSPSYGLAENCTFVSTAWCGG--GGFPAMPSYRKLLPSGRLRDGMCKEI 240

Query: 407 KVVVVNGETGEVVEDGVEGEIWVSSPSNASGYLGHPSLTKKTFHSKINNKSSPNFIRTGD 466
           +VVVVN ETGEVVEDGVEGEIW+SSPSNA GYLGHPSLT++TFH K+ NK+S NF+RTGD
Sbjct: 241 EVVVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPSLTEETFHRKLKNKASLNFVRTGD 300

Query: 467 RGVIRGADRFLFVIGRCSDLIKFNNTQQEIHPHYIESTAYNNSSAYLRGGCIAAVKISE- 526
           RGVI+G+DRFLFVIGRCSD+IKFN+  Q+IHPHYIEST+Y N SAYLRGGCIAAVKIS  
Sbjct: 301 RGVIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHYIESTSYKNFSAYLRGGCIAAVKISSG 360

Query: 527 TIAVVAEMQRDDKNDAELLRKICEEIRKAVSIEEGTELGLVVLVKRGNIPKTTSGKVKRW 586
           T+A+VAEMQRDD+NDAELLRKICEEIRKAV IEEG ELG+VVLVKRGN+ KTTSGKVKRW
Sbjct: 361 TVALVAEMQRDDRNDAELLRKICEEIRKAVLIEEGIELGVVVLVKRGNVSKTTSGKVKRW 420

Query: 587 AVKEKLAGGGLGVLMAIGFEKNCE---VLQREEELRTRPALLSLL 627
            VKEKL GGGL VLMA+ F K+CE    L+R+ E  TRP LLS+L
Sbjct: 421 VVKEKLGGGGLEVLMAVEFGKDCEGLKDLERKGEFGTRPVLLSML 463