Bhi03G002319 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi03G002319
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	Cysteine proteinase inhibitor
Location	chr3 : 71711084 .. 71711529 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTGTTGAACCACATCCCCCTTTGCATCCTATTGAGGATATCAATGATACACACGTGCAAGAAATAGGAAGGTTGGCGGTGGAAGACCACAACAAGAAAACAGGAGACAATTTGAAGTTCGTGCGTGTGGTGAATGGTTTGAGTGGAGAAACCCTTCGAATTGGGTGGGAAAATAAAAGTATTATATCACCTTGTGGTGGAGGCTGAAACTACCGATAGAATTAATTGGACATATGTGGCGAAGGTGTTAGAAGTTCTCGGAAACGGCCCATTATTTGATTATCTGTTCGAATCTTTTGAACCCGTTCTCCCCTACAACAACTGCAAGCCTTGATATTGGCCTCATCTCTCTACTTACCTTAAATAGGTTATACCCTTAAATACTTTATCTATGAATAATGTTGGCACGGTATAAATAAAATACTAGATACTTATCTATTAAA

mRNA sequence

ATGAGTGTTGAACCACATCCCCCTTTGCATCCTATTGAGGATATCAATGATACACACGTGCAAGAAATAGGAAGGTTGGCGGTGGAAGACCACAACAAGAAAACAGGAGACAATTTGAAGTTCGTGCGTGTGGTGAATGGTTTGAGTGGAGAAACCCTTCGAATTGGGTGGGAAAATAAAAGTATTATATCACCTTGTGGTGGAGGCTGAAACTACCGATAGAATTAATTGGACATATGTGGCGAAGGTGTTAGAAGTTCTCGGAAACGGCCCATTATTTGATTATCTGTTCGAATCTTTTGAACCCGTTCTCCCCTACAACAACTGCAAGCCTTGATATTGGCCTCATCTCTCTACTTACCTTAAATAGGTTATACCCTTAAATACTTTATCTATGAATAATGTTGGCACGGTATAAATAAAATACTAGATACTTATCTATTAAA

Coding sequence (CDS)

ATGAGTGTTGAACCACATCCCCCTTTGCATCCTATTGAGGATATCAATGATACACACGTGCAAGAAATAGGAAGGTTGGCGGTGGAAGACCACAACAAGAAAACAGGAGACAATTTGAAGTTCGTGCGTGTGGTGAATGGTTTGAGTGGAGAAACCCTTCGAATTGGGTGGGAAAATAAAAGTATTATATCACCTTGTGGTGGAGGCTGA

Protein sequence

MSVEPHPPLHPIEDINDTHVQEIGRLAVEDHNKKTGDNLKFVRVVNGLSGETLRIGWENKSIISPCGGG

Homology

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BLAST of Bhi03G002319 vs. TrEMBL
Match: tr|A0A1S3B5B9|A0A1S3B5B9_CUCME (Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103486185 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 9.3e-08
Identity = 35/59 (59.32%), Postives = 40/59 (67.80%), Query Frame = 0


Query: 11 PIEDINDT-HVQEIGRLAVEDHNKKTGDNLKFVRVVNGLSGETLRIGWENKSIISPCGG 69
          PIEDIND   VQ++G L V+DHNKKTG+NLKFVRVVNGL+             ISP GG
Sbjct: 9  PIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASF-------SDPISPLGG 60

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BLAST of Bhi03G002319 vs. TrEMBL
Match: tr|A0A0A0L191|A0A0A0L191_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE=4 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 3.9e-06
Identity = 33/53 (62.26%), Postives = 38/53 (71.70%), Query Frame = 0


Query: 1  MSVEP-HPPLHPIEDINDTHVQEIGRLAVEDHNK-KTGDNLKFVRVVNGLSGE 52
          MS+E  H  L PI DIND HVQ IG+LAVEDH+    G+ LKFVRVVNGL  +
Sbjct: 1  MSIEEIHHRLKPIIDINDPHVQIIGKLAVEDHDSHNPGEKLKFVRVVNGLKSD 53

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BLAST of Bhi03G002319 vs. TrEMBL
Match: tr|A0A0A0L3W1|A0A0A0L3W1_CUCSA (Proteinase inhibitor I25, cystatin OS=Cucumis sativus OX=3659 GN=Csa_4G508490 PE=4 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.1e-05
Identity = 39/67 (58.21%), Postives = 47/67 (70.15%), Query Frame = 0


Query: 1  MSV-EPHPPLHPIEDIN-DTHVQE-IGRLAVEDHNKKTGDNLKFVRVVNGL-SGETLRIG 60
          MSV E +    PIEDIN D  VQ  IG+LAVEDHNKKTGDNL+ V VVNGL SG  ++ G
Sbjct: 10 MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPG 69

Query: 61 WENKSII 64
            ++ I+
Sbjct: 70 STHEGIL 76

[Close]

BLAST of Bhi03G002319 vs. TrEMBL
Match: tr|A0A1D1XZ13|A0A1D1XZ13_9ARAE (Cysteine proteinase inhibitor (Fragment) OS=Anthurium amnicola OX=1678845 GN=Os03g0429000_0 PE=3 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 5.7e-05
Identity = 25/46 (54.35%), Postives = 35/46 (76.09%), Query Frame = 0


Query: 6   HPPL----HPIEDINDTHVQEIGRLAVEDHNKKTGDNLKFVRVVNG 48
           HPPL     PI+D+ND H++EIG+ AV +HN++ G +L FVRVV+G
Sbjct: 58  HPPLVGAYQPIKDVNDPHIKEIGQYAVSEHNRRAGLSLAFVRVVSG 103

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BLAST of Bhi03G002319 vs. TrEMBL
Match: tr|A0A0A0KZ98|A0A0A0KZ98_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508510 PE=4 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.3e-04
Identity = 27/44 (61.36%), Postives = 34/44 (77.27%), Query Frame = 0


Query: 9  LHPIEDINDTHVQEIGRLAVED-HNKKTGDNLKFVRVVNGLSGE 52
          L PI DIND HVQ +G LAVE+ ++K +G+ LKFVRVVNGL  +
Sbjct: 10 LKPIIDINDPHVQALGELAVENLYSKNSGEKLKFVRVVNGLKSD 53

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BLAST of Bhi03G002319 vs. NCBI nr
Match: XP_008442277.1 (PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo])

HSP 1 Score: 64.7 bits (156), Expect = 1.4e-07
Identity = 35/59 (59.32%), Postives = 40/59 (67.80%), Query Frame = 0


Query: 11 PIEDINDT-HVQEIGRLAVEDHNKKTGDNLKFVRVVNGLSGETLRIGWENKSIISPCGG 69
          PIEDIND   VQ++G L V+DHNKKTG+NLKFVRVVNGL+             ISP GG
Sbjct: 9  PIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASF-------SDPISPLGG 60

[Close]

BLAST of Bhi03G002319 vs. NCBI nr
Match: KGN54824.1 (hypothetical protein Csa_4G508520 [Cucumis sativus])

HSP 1 Score: 59.3 bits (142), Expect = 5.9e-06
Identity = 33/53 (62.26%), Postives = 38/53 (71.70%), Query Frame = 0


Query: 1  MSVEP-HPPLHPIEDINDTHVQEIGRLAVEDHNK-KTGDNLKFVRVVNGLSGE 52
          MS+E  H  L PI DIND HVQ IG+LAVEDH+    G+ LKFVRVVNGL  +
Sbjct: 1  MSIEEIHHRLKPIIDINDPHVQIIGKLAVEDHDSHNPGEKLKFVRVVNGLKSD 53

[Close]

BLAST of Bhi03G002319 vs. NCBI nr
Match: KGN54821.1 (Proteinase inhibitor I25, cystatin [Cucumis sativus])

HSP 1 Score: 57.8 bits (138), Expect = 1.7e-05
Identity = 39/67 (58.21%), Postives = 47/67 (70.15%), Query Frame = 0


Query: 1  MSV-EPHPPLHPIEDIN-DTHVQE-IGRLAVEDHNKKTGDNLKFVRVVNGL-SGETLRIG 60
          MSV E +    PIEDIN D  VQ  IG+LAVEDHNKKTGDNL+ V VVNGL SG  ++ G
Sbjct: 10 MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPG 69

Query: 61 WENKSII 64
            ++ I+
Sbjct: 70 STHEGIL 76

[Close]

BLAST of Bhi03G002319 vs. NCBI nr
Match: KGN54823.1 (hypothetical protein Csa_4G508510 [Cucumis sativus])

HSP 1 Score: 54.3 bits (129), Expect = 1.9e-04
Identity = 27/44 (61.36%), Postives = 34/44 (77.27%), Query Frame = 0


Query: 9  LHPIEDINDTHVQEIGRLAVED-HNKKTGDNLKFVRVVNGLSGE 52
          L PI DIND HVQ +G LAVE+ ++K +G+ LKFVRVVNGL  +
Sbjct: 10 LKPIIDINDPHVQALGELAVENLYSKNSGEKLKFVRVVNGLKSD 53

[Close]

BLAST of Bhi03G002319 vs. NCBI nr
Match: XP_021308994.1 (cysteine proteinase inhibitor 8 [Sorghum bicolor] >EER95296.2 hypothetical protein SORBI_3001G461600 [Sorghum bicolor])

HSP 1 Score: 52.0 bits (123), Expect = 9.5e-04
Identity = 24/37 (64.86%), Postives = 28/37 (75.68%), Query Frame = 0


Query: 11 PIEDINDTHVQEIGRLAVEDHNKKTGDNLKFVRVVNG 48
          PI +IND HVQE+GR AV +H KK  D LKF RVV+G
Sbjct: 53 PIVNINDPHVQEVGRWAVSEHVKKANDGLKFSRVVSG 89

The following BLAST results are available for this feature:

BLAST of Bhi03G002319 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Bhi03G002319 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of Bhi03G002319 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3B5B9\|A0A1S3B5B9_CUCME	9.3e-08	59.32	Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103486185 PE=3 SV=1	[more]
tr\|A0A0A0L191\|A0A0A0L191_CUCSA	3.9e-06	62.26	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE=4 SV=1	[more]
tr\|A0A0A0L3W1\|A0A0A0L3W1_CUCSA	1.1e-05	58.21	Proteinase inhibitor I25, cystatin OS=Cucumis sativus OX=3659 GN=Csa_4G508490 PE...	[more]
tr\|A0A1D1XZ13\|A0A1D1XZ13_9ARAE	5.7e-05	54.35	Cysteine proteinase inhibitor (Fragment) OS=Anthurium amnicola OX=1678845 GN=Os0...	[more]
tr\|A0A0A0KZ98\|A0A0A0KZ98_CUCSA	1.3e-04	61.36	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508510 PE=4 SV=1	[more]

BLAST of Bhi03G002319 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008442277.1	1.4e-07	59.32	PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo]	[more]
KGN54824.1	5.9e-06	62.26	hypothetical protein Csa_4G508520 [Cucumis sativus]	[more]
KGN54821.1	1.7e-05	58.21	Proteinase inhibitor I25, cystatin [Cucumis sativus]	[more]
KGN54823.1	1.9e-04	61.36	hypothetical protein Csa_4G508510 [Cucumis sativus]	[more]
XP_021308994.1	9.5e-04	64.86	cysteine proteinase inhibitor 8 [Sorghum bicolor] >EER95296.2 hypothetical prote...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004869	cysteine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR000010	Cystatin_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi03M002319	Bhi03M002319	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 12..47 e-value: 3.3E-10 score: 40.1
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 14..46 e-value: 4.31869E-6 score: 38.6092
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 9..52 e-value: 4.2E-11 score: 44.7
None	No IPR available	SUPERFAMILY	SSF54403	Cystatin/monellin	coord: 11..49

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Bhi03G002319	Csa4G508490	Cucumber (Chinese Long) v2	cuwgoB409
Bhi03G002319	ClCG04G002440	Watermelon (Charleston Gray)	wcgwgoB393
Bhi03G002319	CSPI04G20140	Wild cucumber (PI 183967)	cpiwgoB396
Bhi03G002319	Cucsa.264120	Cucumber (Gy14) v1	cgywgoB540
Bhi03G002319	Lsi01G020650	Bottle gourd (USVL1VR-Ls)	lsiwgoB174
Bhi03G002319	CsGy4G018770	Cucumber (Gy14) v2	cgybwgoB375
Bhi03G002319	CsaV3_4G030330	Cucumber (Chinese Long) v3	cucwgoB398
Bhi03G002319	Cla003015	Watermelon (97103) v1	wgowmB455
Bhi03G002319	Cla97C04G069980	Watermelon (97103) v2	wgowmbB434

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi03G002319	Cucurbita maxima (Rimu)	cmawgoB0796
Bhi03G002319	Cucurbita maxima (Rimu)	cmawgoB1078
Bhi03G002319	Cucurbita moschata (Rifu)	cmowgoB0789
Bhi03G002319	Cucurbita moschata (Rifu)	cmowgoB1071
Bhi03G002319	Cucurbita pepo (Zucchini)	cpewgoB0090
Bhi03G002319	Cucurbita pepo (Zucchini)	cpewgoB0605
Bhi03G002319	Wild cucumber (PI 183967)	cpiwgoB403
Bhi03G002319	Cucumber (Chinese Long) v2	cuwgoB398
Bhi03G002319	Melon (DHL92) v3.6.1	medwgoB663
Bhi03G002319	Melon (DHL92) v3.5.1	mewgoB656
Bhi03G002319	Watermelon (97103) v2	wgowmbB429
Bhi03G002319	Silver-seed gourd	carwgoB0577
Bhi03G002319	Silver-seed gourd	carwgoB0628