Bhi03G002215 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi03G002215
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	Cysteine proteinase inhibitor
Location	chr3 : 66960814 .. 66961433 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAAGTCCTCTCCCATCAAATTGTCTATCAATCATCCCAAACTACAATACAAACAATGGCTAAGTCTCTTTTCCTAGTAGCTTCTCTCCTCATCTTCCTTGTCGCAGTAACGGAGTCACAAAACGCTTCGAGAAAGCAATCCAAAGCCAACGAGTTCATTCCAATCGAGAATATATCAGACCCATACTATCAAAGCCTTGGAGAGTGGGGTGTAAATGAACACAATAAGGAAGTCAAAACTGATCTCAAATTCCAGGAAGTATACAGTGGTGTTTATGAATTTCGAACTGGGTTAAACTACAAACTCTATTTAAGAGCCATAGATGGGATTTAAGTGGCAACTATGTTGCACATGTCTATGTAACATTGCAACATACCTTGCTTCTTATCCAATTCTATGCTATCGATCGTGATGATCTTCGACAAGTCAATGACTCCTCTGCCATTATTTGAATGTCATAAGCTTCTTTAAAATATGTGTGTATATCTAATAATAATAATAAAATAATGTTGTCTCTCTACTTCTACTTTTTTTTTTTCCTTCCAATGTTTTTGAATAATTAAGTGCTCTCAGGTGTGAAGGAATGACAAAGACAATGAAAGTACGATTCTATTCTTT

mRNA sequence

AAGAAGTCCTCTCCCATCAAATTGTCTATCAATCATCCCAAACTACAATACAAACAATGGCTAAGTCTCTTTTCCTAGTAGCTTCTCTCCTCATCTTCCTTGTCGCAGTAACGGAGTCACAAAACGCTTCGAGAAAGCAATCCAAAGCCAACGAGTTCATTCCAATCGAGAATATATCAGACCCATACTATCAAAGCCTTGGAGAGTGGGGTGTAAATGAACACAATAAGGAAGTCAAAACTGATCTCAAATTCCAGGAAGTATACAGTGGTGTTTATGAATTTCGAACTGGGTTAAACTACAAACTCTATTTAAGAGCCATAGATGGGATTTAAGTGGCAACTATGTTGCACATGTCTATGTAACATTGCAACATACCTTGCTTCTTATCCAATTCTATGCTATCGATCGTGATGATCTTCGACAAGTCAATGACTCCTCTGCCATTATTTGAATGTCATAAGCTTCTTTAAAATATGTGTGTATATCTAATAATAATAATAAAATAATGTTGTCTCTCTACTTCTACTTTTTTTTTTTCCTTCCAATGTTTTTGAATAATTAAGTGCTCTCAGGTGTGAAGGAATGACAAAGACAATGAAAGTACGATTCTATTCTTT

Coding sequence (CDS)

ATGGCTAAGTCTCTTTTCCTAGTAGCTTCTCTCCTCATCTTCCTTGTCGCAGTAACGGAGTCACAAAACGCTTCGAGAAAGCAATCCAAAGCCAACGAGTTCATTCCAATCGAGAATATATCAGACCCATACTATCAAAGCCTTGGAGAGTGGGGTGTAAATGAACACAATAAGGAAGTCAAAACTGATCTCAAATTCCAGGAAGTATACAGTGGTGTTTATGAATTTCGAACTGGGTTAAACTACAAACTCTATTTAAGAGCCATAGATGGGATTTAA

Protein sequence

MAKSLFLVASLLIFLVAVTESQNASRKQSKANEFIPIENISDPYYQSLGEWGVNEHNKEVKTDLKFQEVYSGVYEFRTGLNYKLYLRAIDGI

Homology

[Close]

BLAST of Bhi03G002215 vs. Swiss-Prot
Match: sp|Q84WT8|CYT4_ARATH (Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=2)

HSP 1 Score: 50.8 bits (120), Expect = 9.2e-06
Identity = 31/91 (34.07%), Postives = 47/91 (51.65%), Query Frame = 0


Query: 1  MAKSLFLVASLLIFLVAVTESQNASRKQSKANEFIPIENISDPYYQSLGEWGVNEHNKEV 60
          M KSL  ++ +L+ LV+V E               PI+N+SDP   ++ ++ + EHNKE 
Sbjct: 2  MMKSLICLSLILLPLVSVVEGLGGGGGLGSRK---PIKNVSDPDVVAVAKYAIEEHNKES 61

Query: 61 KTDLKFQEVYSGVYEFRTGLNYKLYLRAIDG 92
          K  L F +V  G  +  +G  Y L + A DG
Sbjct: 62 KEKLVFVKVVEGTTQVVSGTKYDLKIAAKDG 89

[Close]

BLAST of Bhi03G002215 vs. Swiss-Prot
Match: sp|Q10J94|CYT8_ORYSJ (Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0429000 PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.0e-04
Identity = 29/91 (31.87%), Postives = 43/91 (47.25%), Query Frame = 0


Query: 1  MAKSLFLVASLLIFLVAVTESQNASRKQSK-ANEFIPIENISDPYYQSLGEWGVNEHNKE 60
          MA+   L+A LL    A       +R        + PI ++ DP+ Q LG W V  H   
Sbjct: 1  MARIPLLLALLLAVSAAAAAQVGGNRGHGPLVGGWSPITDVGDPHIQELGGWAVERHASL 60

Query: 61 VKTDLKFQEVYSGVYEFRTGLNYKLYLRAID 91
              L+F+ V SG  +  +G+NY+L + A D
Sbjct: 61 SSDGLRFRRVTSGEQQVVSGMNYRLVVSASD 91

[Close]

BLAST of Bhi03G002215 vs. TAIR10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein)

HSP 1 Score: 50.8 bits (120), Expect = 5.1e-07
Identity = 31/91 (34.07%), Postives = 47/91 (51.65%), Query Frame = 0


Query: 1  MAKSLFLVASLLIFLVAVTESQNASRKQSKANEFIPIENISDPYYQSLGEWGVNEHNKEV 60
          M KSL  ++ +L+ LV+V E               PI+N+SDP   ++ ++ + EHNKE 
Sbjct: 2  MMKSLICLSLILLPLVSVVEGLGGGGGLGSRK---PIKNVSDPDVVAVAKYAIEEHNKES 61

Query: 61 KTDLKFQEVYSGVYEFRTGLNYKLYLRAIDG 92
          K  L F +V  G  +  +G  Y L + A DG
Sbjct: 62 KEKLVFVKVVEGTTQVVSGTKYDLKIAAKDG 89

[Close]

BLAST of Bhi03G002215 vs. TrEMBL
Match: tr|A0A0A0LT34|A0A0A0LT34_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_1G182070 PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.2e-13
Identity = 41/91 (45.05%), Postives = 62/91 (68.13%), Query Frame = 0


Query: 1  MAKSLFLVASLLIFLVAVTESQNASRKQSKANEFIPIENISDPYYQSLGEWGVNEHNKEV 60
          MA S   +AS+L+F+ +++    A+ +     ++ PI++I+DPY QSLGE+ V EHNKE 
Sbjct: 1  MAGSFCFLASVLVFVASMSSIATATSRLDLVGDYKPIKDIADPYIQSLGEFAVKEHNKEA 60

Query: 61 KTDLKFQEVYSGVYEFRTGLNYKLYLRAIDG 92
          KT+LKF+EV SG  +   G NY+L L A++G
Sbjct: 61 KTELKFKEVISGKLQIVAGTNYELQLTALEG 91

[Close]

BLAST of Bhi03G002215 vs. TrEMBL
Match: tr|A0A0A0LYM0|A0A0A0LYM0_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_1G183070 PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.5e-13
Identity = 42/91 (46.15%), Postives = 61/91 (67.03%), Query Frame = 0


Query: 1  MAKSLFLVASLLIFLVAVTESQNASRKQSKANEFIPIENISDPYYQSLGEWGVNEHNKEV 60
          MA+SL  +ASLL+F+V+++       +      + PI++I+DPY QSLGE+ VNEHNK+ 
Sbjct: 1  MARSLIFMASLLLFIVSMSSVAATRAQLDLLGGYKPIKDIADPYIQSLGEFAVNEHNKQA 60

Query: 61 KTDLKFQEVYSGVYEFRTGLNYKLYLRAIDG 92
          KT+LKFQ+V  G  +   G NY L L A++G
Sbjct: 61 KTELKFQKVIGGKLQIVAGTNYDLRLTALEG 91

[Close]

BLAST of Bhi03G002215 vs. TrEMBL
Match: tr|Q4FZ48|Q4FZ48_MAIZE (Cysteine proteinase inhibitor OS=Zea mays OX=4577 GN=cc9 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.1e-08
Identity = 31/75 (41.33%), Postives = 45/75 (60.00%), Query Frame = 0


Query: 16  VAVTESQNASRKQSKANEFIPIENISDPYYQSLGEWGVNEHNKEVKTDLKFQEVYSGVYE 75
           VA+  S  A+   + + +++ IEN+ DPY Q +GEW V EHN++    L+F EV SG+ +
Sbjct: 72  VAICRSARAAGVTTLSGQYVKIENVKDPYVQGVGEWAVKEHNRQTGESLQFAEVVSGMEQ 131

Query: 76  FRTGLNYKLYLRAID 91
              G NYKL L   D
Sbjct: 132 VVAGTNYKLNLATKD 146

[Close]

BLAST of Bhi03G002215 vs. TrEMBL
Match: tr|F8TP56|F8TP56_MAIZE (Cysteine proteinase inhibitor (Fragment) OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.1e-08
Identity = 31/75 (41.33%), Postives = 45/75 (60.00%), Query Frame = 0


Query: 16  VAVTESQNASRKQSKANEFIPIENISDPYYQSLGEWGVNEHNKEVKTDLKFQEVYSGVYE 75
           VA+  S  A+   + + +++ IEN+ DPY Q +GEW V EHN++    L+F EV SG+ +
Sbjct: 75  VAICRSARAAGVTTLSGQYVKIENVKDPYVQGVGEWAVKEHNRQTGESLQFAEVVSGMEQ 134

Query: 76  FRTGLNYKLYLRAID 91
              G NYKL L   D
Sbjct: 135 VVAGTNYKLNLATKD 149

[Close]

BLAST of Bhi03G002215 vs. TrEMBL
Match: tr|A0A1U8JRS8|A0A1U8JRS8_GOSHI (Cysteine proteinase inhibitor OS=Gossypium hirsutum OX=3635 GN=LOC107908307 PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.5e-08
Identity = 36/88 (40.91%), Postives = 56/88 (63.64%), Query Frame = 0


Query: 4  SLFLVASLLIFLVAVTESQNASRKQSKANEFIPIENISDPYYQSLGEWGVNEHNKEVKTD 63
          S FL+ SL I L+++T S   +RK + A  + PI++I DP+   + ++ V+E+NK+ K  
Sbjct: 5  SCFLILSLSILLLSLTFSD--ARKDALAGGWTPIKDIKDPHVMEIAKFAVDEYNKQSKVS 64

Query: 64 LKFQEVYSGVYEFRTGLNYKLYLRAIDG 92
          LK  EV  G  +  +G+NYKL L+A DG
Sbjct: 65 LKLDEVVKGETQVVSGINYKLVLKAKDG 90

[Close]

BLAST of Bhi03G002215 vs. NCBI nr
Match: XP_022927105.1 (cysteine proteinase inhibitor 1-like [Cucurbita moschata])

HSP 1 Score: 92.4 bits (228), Expect = 8.4e-16
Identity = 46/91 (50.55%), Postives = 65/91 (71.43%), Query Frame = 0


Query: 1  MAKSLFLVASLLIFLVAVTESQNASRKQSKANEFIPIENISDPYYQSLGEWGVNEHNKEV 60
          M K+LFLVASLL+++++++    A+++   A  + PI+NI DP  QSLGE+ VNEHNK+ 
Sbjct: 1  MTKALFLVASLLLYVLSLSSLVAATQRLDLAGSYKPIKNIDDPKIQSLGEFAVNEHNKQA 60

Query: 61 KTDLKFQEVYSGVYEFRTGLNYKLYLRAIDG 92
          KT LKFQ+V SG  +   G NYKL L A++G
Sbjct: 61 KTQLKFQKVISGEMQIVAGTNYKLQLTALEG 91

[Close]

BLAST of Bhi03G002215 vs. NCBI nr
Match: XP_023520245.1 (cysteine proteinase inhibitor 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 92.0 bits (227), Expect = 1.1e-15
Identity = 46/91 (50.55%), Postives = 65/91 (71.43%), Query Frame = 0


Query: 1  MAKSLFLVASLLIFLVAVTESQNASRKQSKANEFIPIENISDPYYQSLGEWGVNEHNKEV 60
          MAK+LFLVASLL+++++++    A+++   A  + PIENI DP  QSLGE+ VNEHN + 
Sbjct: 1  MAKALFLVASLLLYVLSLSSLAAATQRLDLAGSYKPIENIDDPKIQSLGEFAVNEHNNQA 60

Query: 61 KTDLKFQEVYSGVYEFRTGLNYKLYLRAIDG 92
          KT L+FQ+V SG  +   G NYKL L A++G
Sbjct: 61 KTQLQFQKVISGEMQIVAGTNYKLRLTALEG 91

[Close]

BLAST of Bhi03G002215 vs. NCBI nr
Match: XP_023520258.1 (cysteine proteinase inhibitor 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 89.7 bits (221), Expect = 5.5e-15
Identity = 45/91 (49.45%), Postives = 63/91 (69.23%), Query Frame = 0


Query: 1  MAKSLFLVASLLIFLVAVTESQNASRKQSKANEFIPIENISDPYYQSLGEWGVNEHNKEV 60
          MAK LFLVASLL++++++     A+++   A  + PIENI DP  QSLGE+ VNEHNK+ 
Sbjct: 1  MAKVLFLVASLLLYVLSLLSLAAATQRLDLAGSYKPIENIDDPKIQSLGEFAVNEHNKQA 60

Query: 61 KTDLKFQEVYSGVYEFRTGLNYKLYLRAIDG 92
          KT ++FQ+V  G  +   G NYKL L A++G
Sbjct: 61 KTQIQFQKVIGGEMQIVAGTNYKLRLTALEG 91

[Close]

BLAST of Bhi03G002215 vs. NCBI nr
Match: XP_022142674.1 (cysteine proteinase inhibitor 1-like [Momordica charantia])

HSP 1 Score: 88.2 bits (217), Expect = 1.6e-14
Identity = 45/91 (49.45%), Postives = 65/91 (71.43%), Query Frame = 0


Query: 1  MAKSLFLVASLLIFLVAVTESQNASRKQSKANEFIPIENISDPYYQSLGEWGVNEHNKEV 60
          MAKSLFLVASLL+ L A     + +R+++ A  +  I+N++DPY QSLG++ V EHN++ 
Sbjct: 1  MAKSLFLVASLLVLLAAAV---SGARREALAGGYEAIKNVNDPYIQSLGKFAVEEHNRKA 60

Query: 61 KTDLKFQEVYSGVYEFRTGLNYKLYLRAIDG 92
          K DLKF++V SG+ +   G NY L ++AIDG
Sbjct: 61 KDDLKFEKVKSGLVQIVDGTNYSLQVQAIDG 88

[Close]

BLAST of Bhi03G002215 vs. NCBI nr
Match: XP_023520817.1 (cysteine proteinase inhibitor 1-like [Cucurbita pepo subsp. pepo] >XP_023521297.1 cysteine proteinase inhibitor 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 87.8 bits (216), Expect = 2.1e-14
Identity = 44/91 (48.35%), Postives = 62/91 (68.13%), Query Frame = 0


Query: 1  MAKSLFLVASLLIFLVAVTESQNASRKQSKANEFIPIENISDPYYQSLGEWGVNEHNKEV 60
          MAK+LFL ASLL+++++++    A+ +      + PIENI DP  QSLGE+ VNEHNK+ 
Sbjct: 1  MAKTLFLAASLLLYVLSLSSLAAATNRLDLVGSYKPIENIDDPKIQSLGEFTVNEHNKQA 60

Query: 61 KTDLKFQEVYSGVYEFRTGLNYKLYLRAIDG 92
          KT L+FQ+V SG  +   G NY L L A++G
Sbjct: 61 KTQLQFQKVISGEMQIVAGTNYNLRLTALEG 91

The following BLAST results are available for this feature:

BLAST of Bhi03G002215 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q84WT8\|CYT4_ARATH	9.2e-06	34.07	Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=...	[more]
sp\|Q10J94\|CYT8_ORYSJ	1.0e-04	31.87	Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03...	[more]

BLAST of Bhi03G002215 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G16500.1	5.1e-07	34.07	Cystatin/monellin superfamily protein	[more]

BLAST of Bhi03G002215 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LT34\|A0A0A0LT34_CUCSA	1.2e-13	45.05	Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_1G182070 PE=3 SV...	[more]
tr\|A0A0A0LYM0\|A0A0A0LYM0_CUCSA	1.5e-13	46.15	Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_1G183070 PE=3 SV...	[more]
tr\|Q4FZ48\|Q4FZ48_MAIZE	1.1e-08	41.33	Cysteine proteinase inhibitor OS=Zea mays OX=4577 GN=cc9 PE=2 SV=1	[more]
tr\|F8TP56\|F8TP56_MAIZE	1.1e-08	41.33	Cysteine proteinase inhibitor (Fragment) OS=Zea mays OX=4577 PE=2 SV=1	[more]
tr\|A0A1U8JRS8\|A0A1U8JRS8_GOSHI	2.5e-08	40.91	Cysteine proteinase inhibitor OS=Gossypium hirsutum OX=3635 GN=LOC107908307 PE=3...	[more]

BLAST of Bhi03G002215 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022927105.1	8.4e-16	50.55	cysteine proteinase inhibitor 1-like [Cucurbita moschata]	[more]
XP_023520245.1	1.1e-15	50.55	cysteine proteinase inhibitor 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_023520258.1	5.5e-15	49.45	cysteine proteinase inhibitor 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022142674.1	1.6e-14	49.45	cysteine proteinase inhibitor 1-like [Momordica charantia]	[more]
XP_023520817.1	2.1e-14	48.35	cysteine proteinase inhibitor 1-like [Cucurbita pepo subsp. pepo] >XP_023521297....	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004869	cysteine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR027214	Cystatin
IPR000010	Cystatin_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi03M002215	Bhi03M002215	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 37..91 e-value: 1.0E-10 score: 41.7
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 30..91 e-value: 7.8E-10 score: 40.8
None	No IPR available	PANTHER	PTHR11413:SF65	CYSTEINE PROTEINASE INHIBITOR 5	coord: 4..90
None	No IPR available	PRODOM	PD001231		coord: 46..90 e-value: 1.0E-5 score: 111.0
None	No IPR available	SUPERFAMILY	SSF54403	Cystatin/monellin	coord: 34..91
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 4..90

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi03G002215	Cucumber (Gy14) v1	cgywgoB712
Bhi03G002215	Cucumber (Gy14) v2	cgybwgoB390
Bhi03G002215	Cucurbita maxima (Rimu)	cmawgoB0795
Bhi03G002215	Cucurbita maxima (Rimu)	cmawgoB1066
Bhi03G002215	Cucurbita maxima (Rimu)	cmawgoB1075
Bhi03G002215	Cucurbita moschata (Rifu)	cmowgoB0788
Bhi03G002215	Cucurbita moschata (Rifu)	cmowgoB1060
Bhi03G002215	Cucurbita moschata (Rifu)	cmowgoB1068
Bhi03G002215	Cucurbita pepo (Zucchini)	cpewgoB0104
Bhi03G002215	Cucurbita pepo (Zucchini)	cpewgoB0601
Bhi03G002215	Bottle gourd (USVL1VR-Ls)	lsiwgoB173
Bhi03G002215	Bottle gourd (USVL1VR-Ls)	lsiwgoB188
Bhi03G002215	Melon (DHL92) v3.6.1	medwgoB667
Bhi03G002215	Melon (DHL92) v3.5.1	mewgoB642
Bhi03G002215	Melon (DHL92) v3.5.1	mewgoB661
Bhi03G002215	Watermelon (Charleston Gray)	wcgwgoB387
Bhi03G002215	Watermelon (Charleston Gray)	wcgwgoB397
Bhi03G002215	Watermelon (97103) v2	wgowmbB427
Bhi03G002215	Watermelon (97103) v2	wgowmbB438
Bhi03G002215	Watermelon (97103) v1	wgowmB441
Bhi03G002215	Silver-seed gourd	carwgoB0370
Bhi03G002215	Silver-seed gourd	carwgoB0373
Bhi03G002215	Silver-seed gourd	carwgoB1000