Bhi03G001607 (gene) Wax gourd

NameBhi03G001607
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionNRT1/PTR family protein 2.2
Locationchr3 : 44142691 .. 44144115 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAGTGCTGTCACTAGGCGCAGGCTTGCCTCAAATGAGACCTCCACCCTGCATCAGTGGTGAATCCAGTTGCCCACAACCTCAACCATGGCAACTCGGCTTCCTCTACCTCGGCCTCGGACTAATCGTCGTCGGAGCCGGAGGTATACGGCCTTGCAACATCTCCTTCGGCGCCGACCAATTCGACACCAACACTGAAAAGGGCAGAGCCAAACTCGAAAGCTTCTTAAACTGGTGGTACTTGCTCTTCTCCATCGCCCTTGTCATAGCACTAACCATCGTCGTATATGTCCAAACCAACATCAGTTGGACCCTCGGCTTCGCCATTCCGACTGCCTGCTTCGTCGTATCGATCCTCATCTTCCTCTCTGGCCGTCGTTTTTACGTCTGCAAAGCACCACAAGGCAGTGTCTTTTCAGACTTAGCAAAAGTCGTCGTAGCGATGTTCCGAAAGCGAAAAATCCCAAAACCAAATCAAATTGATCACTTACATAACCCTCCAATGAATTCATGTAAGTTAGCTCACACAGACAGGTTTTTGGTATTTGACAAAGCGGCTACAGTGGTTGATTCCTCCATTGAATTAGACGAAGAAGGGAAATCAAAGGACGAATGGAGATTATGTAGTGTTCATCAAGTTGAACAATTTAAATGCGTAGTTGGGATTATCCCTGTTTGGTTAGCTGGAATTGCATGCTTTATCTCAATGCAACAAATGGGTTCATTTGGGATTTTACAAGCCATTCAAATGAACAGATTTATAGGACCTCATTTCCAAATCCCACCAGCTTGGATGAGTTTAACTCCCATGATTGCTCTATCAATTTGGATTTACATTTACGAGAAATACGTCGAATTCATGAAGAAAAAAACCCATAGCAACAAACGTTTCTCCATGAAAACAAGAATTGAAATTGGGATTGTGATGTCGGTTCTATGTATGGTGGTAGCTGGACTTCTGGAGAAGCTCCGCCGTGATGCGGCAGTCGAGAATAAGTCGTTTGTATCGCCGTTACATGTTTGGATGTTAATACCAGAGTTTGCTCTGTCTGGATTAACAGAGGCATTTGCTGCGATTGCGGTGATGGAGCTTTTGACGACGCATTTGCCGGAGAGCCTGAGGACAGTGGCCGGAGCGATATTTTTCTTGAGTTTGTCGTTGGCGAGTTACTTGAGCTCTGTTTTGATGGGGATAGTGAGCAAAATAGGGAGAATGGAAGCAGGAGAAATGCAATGGCTTGGAGGAAATGATTTGAATAAAAATAGGCTCGATTATTTCTTCTATGTAGTTGGTGGGATTGCAGCTTTGAATTTCTTCTACTTTCGATTCTTTGCTGCTGGGTTTTTACCAAGCCCCGATGTTGATCCGCGGCAGAAGGCACATCAAAGGCAGTTAGAGGACGCAGAATTGGGTCGTAGATAA

mRNA sequence

ATGGGAGTGCTGTCACTAGGCGCAGGCTTGCCTCAAATGAGACCTCCACCCTGCATCAGTGGTGAATCCAGTTGCCCACAACCTCAACCATGGCAACTCGGCTTCCTCTACCTCGGCCTCGGACTAATCGTCGTCGGAGCCGGAGGTATACGGCCTTGCAACATCTCCTTCGGCGCCGACCAATTCGACACCAACACTGAAAAGGGCAGAGCCAAACTCGAAAGCTTCTTAAACTGGTGGTACTTGCTCTTCTCCATCGCCCTTGTCATAGCACTAACCATCGTCGTATATGTCCAAACCAACATCAGTTGGACCCTCGGCTTCGCCATTCCGACTGCCTGCTTCGTCGTATCGATCCTCATCTTCCTCTCTGGCCGTCGTTTTTACGTCTGCAAAGCACCACAAGGCAGTGTCTTTTCAGACTTAGCAAAAGTCGTCGTAGCGATGTTCCGAAAGCGAAAAATCCCAAAACCAAATCAAATTGATCACTTACATAACCCTCCAATGAATTCATGTAAGTTAGCTCACACAGACAGGTTTTTGGTATTTGACAAAGCGGCTACAGTGGTTGATTCCTCCATTGAATTAGACGAAGAAGGGAAATCAAAGGACGAATGGAGATTATGTAGTGTTCATCAAGTTGAACAATTTAAATGCGTAGTTGGGATTATCCCTGTTTGGTTAGCTGGAATTGCATGCTTTATCTCAATGCAACAAATGGGTTCATTTGGGATTTTACAAGCCATTCAAATGAACAGATTTATAGGACCTCATTTCCAAATCCCACCAGCTTGGATGAGTTTAACTCCCATGATTGCTCTATCAATTTGGATTTACATTTACGAGAAATACGTCGAATTCATGAAGAAAAAAACCCATAGCAACAAACGTTTCTCCATGAAAACAAGAATTGAAATTGGGATTGTGATGTCGGTTCTATGTATGGTGGTAGCTGGACTTCTGGAGAAGCTCCGCCGTGATGCGGCAGTCGAGAATAAGTCGTTTGTATCGCCGTTACATGTTTGGATGTTAATACCAGAGTTTGCTCTGTCTGGATTAACAGAGGCATTTGCTGCGATTGCGGTGATGGAGCTTTTGACGACGCATTTGCCGGAGAGCCTGAGGACAGTGGCCGGAGCGATATTTTTCTTGAGTTTGTCGTTGGCGAGTTACTTGAGCTCTGTTTTGATGGGGATAGTGAGCAAAATAGGGAGAATGGAAGCAGGAGAAATGCAATGGCTTGGAGGAAATGATTTGAATAAAAATAGGCTCGATTATTTCTTCTATGTAGTTGGTGGGATTGCAGCTTTGAATTTCTTCTACTTTCGATTCTTTGCTGCTGGGTTTTTACCAAGCCCCGATGTTGATCCGCGGCAGAAGGCACATCAAAGGCAGTTAGAGGACGCAGAATTGGGTCGTAGATAA

Coding sequence (CDS)

ATGGGAGTGCTGTCACTAGGCGCAGGCTTGCCTCAAATGAGACCTCCACCCTGCATCAGTGGTGAATCCAGTTGCCCACAACCTCAACCATGGCAACTCGGCTTCCTCTACCTCGGCCTCGGACTAATCGTCGTCGGAGCCGGAGGTATACGGCCTTGCAACATCTCCTTCGGCGCCGACCAATTCGACACCAACACTGAAAAGGGCAGAGCCAAACTCGAAAGCTTCTTAAACTGGTGGTACTTGCTCTTCTCCATCGCCCTTGTCATAGCACTAACCATCGTCGTATATGTCCAAACCAACATCAGTTGGACCCTCGGCTTCGCCATTCCGACTGCCTGCTTCGTCGTATCGATCCTCATCTTCCTCTCTGGCCGTCGTTTTTACGTCTGCAAAGCACCACAAGGCAGTGTCTTTTCAGACTTAGCAAAAGTCGTCGTAGCGATGTTCCGAAAGCGAAAAATCCCAAAACCAAATCAAATTGATCACTTACATAACCCTCCAATGAATTCATGTAAGTTAGCTCACACAGACAGGTTTTTGGTATTTGACAAAGCGGCTACAGTGGTTGATTCCTCCATTGAATTAGACGAAGAAGGGAAATCAAAGGACGAATGGAGATTATGTAGTGTTCATCAAGTTGAACAATTTAAATGCGTAGTTGGGATTATCCCTGTTTGGTTAGCTGGAATTGCATGCTTTATCTCAATGCAACAAATGGGTTCATTTGGGATTTTACAAGCCATTCAAATGAACAGATTTATAGGACCTCATTTCCAAATCCCACCAGCTTGGATGAGTTTAACTCCCATGATTGCTCTATCAATTTGGATTTACATTTACGAGAAATACGTCGAATTCATGAAGAAAAAAACCCATAGCAACAAACGTTTCTCCATGAAAACAAGAATTGAAATTGGGATTGTGATGTCGGTTCTATGTATGGTGGTAGCTGGACTTCTGGAGAAGCTCCGCCGTGATGCGGCAGTCGAGAATAAGTCGTTTGTATCGCCGTTACATGTTTGGATGTTAATACCAGAGTTTGCTCTGTCTGGATTAACAGAGGCATTTGCTGCGATTGCGGTGATGGAGCTTTTGACGACGCATTTGCCGGAGAGCCTGAGGACAGTGGCCGGAGCGATATTTTTCTTGAGTTTGTCGTTGGCGAGTTACTTGAGCTCTGTTTTGATGGGGATAGTGAGCAAAATAGGGAGAATGGAAGCAGGAGAAATGCAATGGCTTGGAGGAAATGATTTGAATAAAAATAGGCTCGATTATTTCTTCTATGTAGTTGGTGGGATTGCAGCTTTGAATTTCTTCTACTTTCGATTCTTTGCTGCTGGGTTTTTACCAAGCCCCGATGTTGATCCGCGGCAGAAGGCACATCAAAGGCAGTTAGAGGACGCAGAATTGGGTCGTAGATAA

Protein sequence

MGVLSLGAGLPQMRPPPCISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSILIFLSGRRFYVCKAPQGSVFSDLAKVVVAMFRKRKIPKPNQIDHLHNPPMNSCKLAHTDRFLVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFISMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHSNKRFSMKTRIEIGIVMSVLCMVVAGLLEKLRRDAAVENKSFVSPLHVWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQWLGGNDLNKNRLDYFFYVVGGIAALNFFYFRFFAAGFLPSPDVDPRQKAHQRQLEDAELGRR
BLAST of Bhi03G001607 vs. Swiss-Prot
Match: sp|Q3E8X3|PTR52_ARATH (Protein NRT1/ PTR FAMILY 2.8 OS=Arabidopsis thaliana OX=3702 GN=NPF2.8 PE=2 SV=2)

HSP 1 Score: 463.4 bits (1191), Expect = 3.1e-129
Identity = 236/456 (51.75%), Postives = 318/456 (69.74%), Query Frame = 0

Query: 1   MGVLSLGAGLPQMRPPPCISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGAD 60
           MG+ +L A LP +RP  CI   +   QP  WQLG L+ GLGL+ +GAGG+RPCNI+FGAD
Sbjct: 103 MGIFALTAALPSLRPDACIDPSNCSNQPAKWQLGVLFSGLGLLAIGAGGVRPCNIAFGAD 162

Query: 61  QFDTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSIL 120
           QFDT+T+KG+A LE+F NWWY  F++ALVIALT VVY+QTNISW +GF IPTAC  +SI 
Sbjct: 163 QFDTSTKKGKAHLETFFNWWYFSFTVALVIALTGVVYIQTNISWVIGFVIPTACLALSIT 222

Query: 121 IFLSGRRFYVCKAPQGSVFSDLAKVVVAMFRKRKIPKPNQIDHLHNP-----PMNSCKLA 180
            F+ G+  Y+C   +GSVF+D+ KVV A  +KRK+   + I     P     P    +  
Sbjct: 223 TFVIGQHTYICAKAEGSVFADIVKVVTAACKKRKVKPGSDITFYIGPSNDGSPTTLVRDK 282

Query: 181 HTDRFLVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFI 240
           H  RF  FDKA+ V + + EL+E+G +K +WRLCSV QV+  KCV  I+PVW+ GIACFI
Sbjct: 283 HRLRF--FDKASIVTNPN-ELNEDGNAKYKWRLCSVQQVKNLKCVTAILPVWVTGIACFI 342

Query: 241 SMQQMGSFGILQAIQMNRFIGPH-FQIPPAWMSLTPMIALSIWIYIYE-KYVEFMKKKTH 300
              Q   +GILQA+QM++  GPH FQ+P  WM+L  MI L+IWI +YE   +  +K+ T 
Sbjct: 343 LTDQQNIYGILQAMQMDKTFGPHNFQVPAGWMNLVSMITLAIWISLYECVIIPIVKQITG 402

Query: 301 SNKRFSMKTRIEIGIVMSVLCMVVAGLLEKLRRDAAVENKSFVSPLHVWMLIPEFALSGL 360
             KR ++K RIE  IVM ++CM+VAG  EK RR +A++N SFVSP+ + ML+P+FAL+GL
Sbjct: 403 RKKRLTLKHRIE--IVMGIICMIVAGFQEKKRRASALKNGSFVSPVSIVMLLPQFALAGL 462

Query: 361 TEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQW 420
           TEAF+A+A+ME LT  +PE +R VAGAIFFLS S+ASY+ ++L+ ++  + R E     W
Sbjct: 463 TEAFSAVALMEFLTVRMPEHMRAVAGAIFFLSSSIASYICTLLINVIDAVTRKEG--KSW 522

Query: 421 LGGNDLNKNRLDYFFYVVGGIAALNFFYFRFFAAGF 450
           LG  DLNKNRL+ +F+++ GI   N  YFR FA+ +
Sbjct: 523 LGDKDLNKNRLENYFFIIAGIQVANLLYFRLFASRY 551

BLAST of Bhi03G001607 vs. Swiss-Prot
Match: sp|Q8RX77|PTR21_ARATH (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 1.5e-88
Identity = 175/456 (38.38%), Postives = 283/456 (62.06%), Query Frame = 0

Query: 4   LSLGAGLPQMRPPPCISGES-SCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGADQF 63
           ++L A  PQ+ P  C S +  SC  P   Q+G L LGL  + VG+GGIRPC+I FG DQF
Sbjct: 140 ITLTASFPQLHPASCNSQDPLSCGGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQF 199

Query: 64  DTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSILIF 123
           D  TE+G   + SF NW+Y+ F++ L+I  T+VVY+Q  +SW +GF+IPT    +++++F
Sbjct: 200 DQRTEEGVKGVASFFNWYYMTFTVVLIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMF 259

Query: 124 LSGRRFYVCKAPQGSVFSDLAKVVVAMFRKRKIPKPNQID---HLHNPPMNS---CKLAH 183
            +G + YV   P+GS+FS +A+V+VA  +KRK+  P + D     ++P + S    KL  
Sbjct: 260 FAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHR 319

Query: 184 TDRFLVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFIS 243
           +++F   DKAA V++   +L  EG   D+WRLCSV +VE+ KC++ I+P+W AGI    +
Sbjct: 320 SNQFRCLDKAAVVIEG--DLTPEGPPADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAA 379

Query: 244 MQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEK-YVEFMKKKTHSN 303
           M   G+F + QA++M+R +GP F+IP   +S+  ++ + I++  Y++ +V FM++ T   
Sbjct: 380 MTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHK 439

Query: 304 KRFSMKTRIEIGIVMSVLCMVVAGLLEKLRRDAAVE--NKSFVSPLHVWMLIPEFALSGL 363
              ++  RI  GIV ++  M+VAG++E++RR  ++   + + ++P+ V+ L P+  L GL
Sbjct: 440 SGITLLQRIGTGIVFAIFSMIVAGIVERMRRIRSINAGDPTGMTPMSVFWLSPQLILMGL 499

Query: 364 TEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQW 423
            EAF  I  +E   +  PE +R++A ++F LS + +SYLSS L+ +V K          W
Sbjct: 500 CEAFNIIGQIEFFNSQFPEHMRSIANSLFSLSFAGSSYLSSFLVTVVHKFSGGH-DRPDW 559

Query: 424 LGGNDLNKNRLDYFFYVVGGIAALNFFYFRFFAAGF 450
           L  N LN  +LDYF+Y++  +  +N  YF + A G+
Sbjct: 560 LNKN-LNAGKLDYFYYLIAVLGVVNLVYFWYCARGY 591

BLAST of Bhi03G001607 vs. Swiss-Prot
Match: sp|Q9LFX9|PTR13_ARATH (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 320.5 bits (820), Expect = 3.2e-86
Identity = 179/452 (39.60%), Postives = 278/452 (61.50%), Query Frame = 0

Query: 4   LSLGAGLPQMRPPPCISGE-SSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGADQF 63
           ++L A LPQ+ PPPC +     C  P   QLG L+LGLG + +G+GGIRPC+I FG DQF
Sbjct: 103 VTLTACLPQLHPPPCNNPHPDECDDPNKLQLGILFLGLGFLSIGSGGIRPCSIPFGVDQF 162

Query: 64  DTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSILIF 123
           D  TE+G   + SF NW+YL  ++ L+ + T+VVY+QT +SW +GF+IPT+    ++++F
Sbjct: 163 DQRTEQGLKGVASFFNWYYLTLTMVLIFSHTVVVYLQT-VSWVIGFSIPTSLMACAVVLF 222

Query: 124 LSGRRFYVCKAPQGSVFSDLAKVVVAMFRKRKIPKPNQID----HLHNPPMNS---CKLA 183
             G RFYV   P+GSVFS +A+V+VA  +KR + K + +D      + PP+      KL 
Sbjct: 223 FVGMRFYVYVKPEGSVFSGIARVIVAARKKRDL-KISLVDDGTEEYYEPPVKPGVLSKLP 282

Query: 184 HTDRFLVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFI 243
            TD+F   DKAA ++D   +L  EG   ++WRLCS+ +VE+ KC++ ++PVW AGI   +
Sbjct: 283 LTDQFKFLDKAAVILDG--DLTSEGVPANKWRLCSIQEVEEVKCLIRVVPVWSAGIISIV 342

Query: 244 SMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEK-YVEFMKKKTHS 303
           +M    +F + QA +M+R +GPHF+IP A +++   I + IW+ IYE   V F+ +    
Sbjct: 343 AMTTQATFMVFQATKMDRHMGPHFEIPAASITVISYITIGIWVPIYEHLLVPFLWRM--R 402

Query: 304 NKRFSMKTRIEIGIVMSVLCMVVAGLLEKLRRDAAVENKSFVSPLHVWMLIPEFALSGLT 363
             R ++  R+ IGIV ++L M  AG +E +RR  A E    ++ + V+ L     L GL 
Sbjct: 403 KFRVTLLQRMGIGIVFAILSMFTAGFVEGVRRTRATE----MTQMSVFWLALPLILMGLC 462

Query: 364 EAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQWL 423
           E+F  I ++E   +  PE +R++A ++F LS + A+YLSS+L+  V K+   +     WL
Sbjct: 463 ESFNFIGLIEFFNSQFPEHMRSIANSLFPLSFAAANYLSSLLVTTVHKVSGTK-DHPDWL 522

Query: 424 GGNDLNKNRLDYFFYVVGGIAALNFFYFRFFA 447
              DL++ +LDYF+Y++  +  +N  YF + A
Sbjct: 523 -NKDLDRGKLDYFYYLIAVLGVVNLVYFWYCA 542

BLAST of Bhi03G001607 vs. Swiss-Prot
Match: sp|Q9LV10|PTR53_ARATH (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 2.4e-81
Identity = 168/455 (36.92%), Postives = 272/455 (59.78%), Query Frame = 0

Query: 3   VLSLGAGLPQMRPPPC-ISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGADQ 62
           V+ L A +PQ+ P  C  + +S C  P   Q+ FL +GLG +VVGAGGIRPCN++FGADQ
Sbjct: 131 VILLTAAVPQLHPAACGTAADSICNGPSGGQIAFLLMGLGFLVVGAGGIRPCNLAFGADQ 190

Query: 63  FDTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSILI 122
           F+  +E G+  ++SF NW++  F+ A +++LT+VVYVQ+N+SWT+G  IP     ++ LI
Sbjct: 191 FNPKSESGKRGIDSFFNWYFFTFTFAQILSLTLVVYVQSNVSWTIGLTIPAVLMFLACLI 250

Query: 123 FLSGRRFYVCKAPQGSVFSDLAKVVVAMFRKRKIPKPNQ----IDHLHNPPMNSCKLAHT 182
           F +G + YV     GS  + +A+V+    +KR +    Q    + + + P   + KL +T
Sbjct: 251 FFAGDKLYVKIKASGSPLAGIAQVIAVAIKKRGLKPAKQPWLNLYNYYPPKYANSKLKYT 310

Query: 183 DRFLVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFISM 242
           D+F   DKAA ++    +L  +GK  D W+LC++ QVE+ KC+V ++P+W A    ++++
Sbjct: 311 DQFRFLDKAA-ILTPEDKLQPDGKPADPWKLCTMQQVEEVKCIVRVLPIWFASSIYYLTI 370

Query: 243 QQMGSFGILQAIQMNRFIGP-HFQIPPAWMSLTPMIALSIWIYIYEK-YVEFMKKKTHSN 302
            Q  ++ + QA+Q +R +G   F IP A   +  M  ++++I +Y++  V  M++ T  +
Sbjct: 371 TQQMTYPVFQALQSDRRLGSGGFVIPAATYVVFLMTGMTVFIVVYDRVLVPTMRRITGLD 430

Query: 303 KRFSMKTRIEIGIVMSVLCMVVAGLLEKLRRDAAV--------ENKSFVSPLHVWMLIPE 362
              ++  RI  GI  +   +VVAG +E+ RR  A+          K  +S +    LIP+
Sbjct: 431 TGITLLQRIGTGIFFATASLVVAGFVEERRRTFALTKPTLGMAPRKGEISSMSAMWLIPQ 490

Query: 363 FALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRME 422
            +L+G+ EAFAAI  ME      PE++R+ AG+IF++   ++SYL S L+  V +  +  
Sbjct: 491 LSLAGVAEAFAAIGQMEFYYKQFPENMRSFAGSIFYVGGGVSSYLGSFLIATVHRTTQNS 550

Query: 423 AGEMQWLGGNDLNKNRLDYFFYVVGGIAALNFFYF 443
           +G   WL   DLNK RLD F++++ GI A+NF YF
Sbjct: 551 SGG-NWL-AEDLNKGRLDLFYFMIAGILAVNFAYF 582

BLAST of Bhi03G001607 vs. Swiss-Prot
Match: sp|Q944G5|PTR44_ARATH (Protein NRT1/ PTR FAMILY 2.10 OS=Arabidopsis thaliana OX=3702 GN=NPF2.10 PE=1 SV=3)

HSP 1 Score: 289.7 bits (740), Expect = 6.1e-77
Identity = 162/459 (35.29%), Postives = 270/459 (58.82%), Query Frame = 0

Query: 3   VLSLGAGLPQMRPPPCISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGADQF 62
           V+ L A +P + P  C   + SC  P   Q+ FL +GLG +VVGAGGIRPCN++FGADQF
Sbjct: 149 VILLTAAIPSLHPVAC-GNKISCEGPSVGQILFLLMGLGFLVVGAGGIRPCNLAFGADQF 208

Query: 63  DTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSILIF 122
           +  +E G+  + SF NW++  F+ A +I+LT VVY+Q+N+SWT+G  IP A   ++ +IF
Sbjct: 209 NPKSESGKKGINSFFNWYFFTFTFAQIISLTAVVYIQSNVSWTIGLIIPVALMFLACVIF 268

Query: 123 LSGRRFYVCKAPQGSVFSDLAKVVVAMFRKR-----KIPKPNQIDHLHNPPMNSCKLAHT 182
            +G R YV     GS  + +A+V+ A  +KR     K P  N  +H+ +   N+  L +T
Sbjct: 269 FAGDRLYVKVKASGSPLAGIARVIAAAIKKRGLKPVKQPWVNLYNHIPSNYANT-TLKYT 328

Query: 183 DRFLVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFISM 242
           D+F   DKAA ++    +L+ +G + D W+LC++ QVE+ KC+V +IP+W A    ++++
Sbjct: 329 DQFRFLDKAA-IMTPEEKLNSDGTASDPWKLCTLQQVEEVKCIVRVIPIWFASTIYYLAI 388

Query: 243 QQMGSFGILQAIQMNRFIGP-HFQIPPAWMSLTPMIALSIWIYIYEK-YVEFMKKKTHSN 302
               ++ + QA+Q +R +G   F+IP A   +  M  ++++I  Y++  V  +++ T   
Sbjct: 389 TIQMTYPVFQALQSDRRLGSGGFRIPAATYVVFLMTGMTVFIIFYDRVLVPSLRRVTGLE 448

Query: 303 KRFSMKTRIEIGIVMSVLCMVVAGLLEKLRRDAAVENKSF--------VSPLHVWMLIPE 362
              S+  RI  G   +++ ++V+G +E+ RR+ A+   +         +S +    LIP+
Sbjct: 449 TGISLLQRIGAGFTFAIMSLLVSGFIEERRRNFALTKPTLGMAPRTGEISSMSALWLIPQ 508

Query: 363 FALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRME 422
             L+G+ EAFAAI  ME      PE++++ AG+IF++   ++SYL+S L+  V +     
Sbjct: 509 LTLAGIAEAFAAIGQMEFYYKQFPENMKSFAGSIFYVGAGVSSYLASFLISTVHRT-TAH 568

Query: 423 AGEMQWLGGNDLNKNRLDYFFYVVGGIAALNFFYFRFFA 447
           +    WL   DLNK +LDYF++++ G+  +N  YF   A
Sbjct: 569 SPSGNWL-AEDLNKAKLDYFYFMLTGLMVVNMAYFLLMA 602

BLAST of Bhi03G001607 vs. TAIR10
Match: AT5G28470.1 (Major facilitator superfamily protein)

HSP 1 Score: 463.4 bits (1191), Expect = 1.7e-130
Identity = 236/456 (51.75%), Postives = 318/456 (69.74%), Query Frame = 0

Query: 1   MGVLSLGAGLPQMRPPPCISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGAD 60
           MG+ +L A LP +RP  CI   +   QP  WQLG L+ GLGL+ +GAGG+RPCNI+FGAD
Sbjct: 103 MGIFALTAALPSLRPDACIDPSNCSNQPAKWQLGVLFSGLGLLAIGAGGVRPCNIAFGAD 162

Query: 61  QFDTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSIL 120
           QFDT+T+KG+A LE+F NWWY  F++ALVIALT VVY+QTNISW +GF IPTAC  +SI 
Sbjct: 163 QFDTSTKKGKAHLETFFNWWYFSFTVALVIALTGVVYIQTNISWVIGFVIPTACLALSIT 222

Query: 121 IFLSGRRFYVCKAPQGSVFSDLAKVVVAMFRKRKIPKPNQIDHLHNP-----PMNSCKLA 180
            F+ G+  Y+C   +GSVF+D+ KVV A  +KRK+   + I     P     P    +  
Sbjct: 223 TFVIGQHTYICAKAEGSVFADIVKVVTAACKKRKVKPGSDITFYIGPSNDGSPTTLVRDK 282

Query: 181 HTDRFLVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFI 240
           H  RF  FDKA+ V + + EL+E+G +K +WRLCSV QV+  KCV  I+PVW+ GIACFI
Sbjct: 283 HRLRF--FDKASIVTNPN-ELNEDGNAKYKWRLCSVQQVKNLKCVTAILPVWVTGIACFI 342

Query: 241 SMQQMGSFGILQAIQMNRFIGPH-FQIPPAWMSLTPMIALSIWIYIYE-KYVEFMKKKTH 300
              Q   +GILQA+QM++  GPH FQ+P  WM+L  MI L+IWI +YE   +  +K+ T 
Sbjct: 343 LTDQQNIYGILQAMQMDKTFGPHNFQVPAGWMNLVSMITLAIWISLYECVIIPIVKQITG 402

Query: 301 SNKRFSMKTRIEIGIVMSVLCMVVAGLLEKLRRDAAVENKSFVSPLHVWMLIPEFALSGL 360
             KR ++K RIE  IVM ++CM+VAG  EK RR +A++N SFVSP+ + ML+P+FAL+GL
Sbjct: 403 RKKRLTLKHRIE--IVMGIICMIVAGFQEKKRRASALKNGSFVSPVSIVMLLPQFALAGL 462

Query: 361 TEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQW 420
           TEAF+A+A+ME LT  +PE +R VAGAIFFLS S+ASY+ ++L+ ++  + R E     W
Sbjct: 463 TEAFSAVALMEFLTVRMPEHMRAVAGAIFFLSSSIASYICTLLINVIDAVTRKEG--KSW 522

Query: 421 LGGNDLNKNRLDYFFYVVGGIAALNFFYFRFFAAGF 450
           LG  DLNKNRL+ +F+++ GI   N  YFR FA+ +
Sbjct: 523 LGDKDLNKNRLENYFFIIAGIQVANLLYFRLFASRY 551

BLAST of Bhi03G001607 vs. TAIR10
Match: AT1G69870.1 (nitrate transporter 1.7)

HSP 1 Score: 328.2 bits (840), Expect = 8.5e-90
Identity = 175/456 (38.38%), Postives = 283/456 (62.06%), Query Frame = 0

Query: 4   LSLGAGLPQMRPPPCISGES-SCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGADQF 63
           ++L A  PQ+ P  C S +  SC  P   Q+G L LGL  + VG+GGIRPC+I FG DQF
Sbjct: 140 ITLTASFPQLHPASCNSQDPLSCGGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQF 199

Query: 64  DTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSILIF 123
           D  TE+G   + SF NW+Y+ F++ L+I  T+VVY+Q  +SW +GF+IPT    +++++F
Sbjct: 200 DQRTEEGVKGVASFFNWYYMTFTVVLIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMF 259

Query: 124 LSGRRFYVCKAPQGSVFSDLAKVVVAMFRKRKIPKPNQID---HLHNPPMNS---CKLAH 183
            +G + YV   P+GS+FS +A+V+VA  +KRK+  P + D     ++P + S    KL  
Sbjct: 260 FAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHR 319

Query: 184 TDRFLVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFIS 243
           +++F   DKAA V++   +L  EG   D+WRLCSV +VE+ KC++ I+P+W AGI    +
Sbjct: 320 SNQFRCLDKAAVVIEG--DLTPEGPPADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAA 379

Query: 244 MQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEK-YVEFMKKKTHSN 303
           M   G+F + QA++M+R +GP F+IP   +S+  ++ + I++  Y++ +V FM++ T   
Sbjct: 380 MTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHK 439

Query: 304 KRFSMKTRIEIGIVMSVLCMVVAGLLEKLRRDAAVE--NKSFVSPLHVWMLIPEFALSGL 363
              ++  RI  GIV ++  M+VAG++E++RR  ++   + + ++P+ V+ L P+  L GL
Sbjct: 440 SGITLLQRIGTGIVFAIFSMIVAGIVERMRRIRSINAGDPTGMTPMSVFWLSPQLILMGL 499

Query: 364 TEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQW 423
            EAF  I  +E   +  PE +R++A ++F LS + +SYLSS L+ +V K          W
Sbjct: 500 CEAFNIIGQIEFFNSQFPEHMRSIANSLFSLSFAGSSYLSSFLVTVVHKFSGGH-DRPDW 559

Query: 424 LGGNDLNKNRLDYFFYVVGGIAALNFFYFRFFAAGF 450
           L  N LN  +LDYF+Y++  +  +N  YF + A G+
Sbjct: 560 LNKN-LNAGKLDYFYYLIAVLGVVNLVYFWYCARGY 591

BLAST of Bhi03G001607 vs. TAIR10
Match: AT1G27080.1 (nitrate transporter 1.6)

HSP 1 Score: 320.5 bits (820), Expect = 1.8e-87
Identity = 179/452 (39.60%), Postives = 278/452 (61.50%), Query Frame = 0

Query: 4   LSLGAGLPQMRPPPCISGE-SSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGADQF 63
           ++L A LPQ+ PPPC +     C  P   QLG L+LGLG + +G+GGIRPC+I FG DQF
Sbjct: 103 VTLTACLPQLHPPPCNNPHPDECDDPNKLQLGILFLGLGFLSIGSGGIRPCSIPFGVDQF 162

Query: 64  DTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSILIF 123
           D  TE+G   + SF NW+YL  ++ L+ + T+VVY+QT +SW +GF+IPT+    ++++F
Sbjct: 163 DQRTEQGLKGVASFFNWYYLTLTMVLIFSHTVVVYLQT-VSWVIGFSIPTSLMACAVVLF 222

Query: 124 LSGRRFYVCKAPQGSVFSDLAKVVVAMFRKRKIPKPNQID----HLHNPPMNS---CKLA 183
             G RFYV   P+GSVFS +A+V+VA  +KR + K + +D      + PP+      KL 
Sbjct: 223 FVGMRFYVYVKPEGSVFSGIARVIVAARKKRDL-KISLVDDGTEEYYEPPVKPGVLSKLP 282

Query: 184 HTDRFLVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFI 243
            TD+F   DKAA ++D   +L  EG   ++WRLCS+ +VE+ KC++ ++PVW AGI   +
Sbjct: 283 LTDQFKFLDKAAVILDG--DLTSEGVPANKWRLCSIQEVEEVKCLIRVVPVWSAGIISIV 342

Query: 244 SMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEK-YVEFMKKKTHS 303
           +M    +F + QA +M+R +GPHF+IP A +++   I + IW+ IYE   V F+ +    
Sbjct: 343 AMTTQATFMVFQATKMDRHMGPHFEIPAASITVISYITIGIWVPIYEHLLVPFLWRM--R 402

Query: 304 NKRFSMKTRIEIGIVMSVLCMVVAGLLEKLRRDAAVENKSFVSPLHVWMLIPEFALSGLT 363
             R ++  R+ IGIV ++L M  AG +E +RR  A E    ++ + V+ L     L GL 
Sbjct: 403 KFRVTLLQRMGIGIVFAILSMFTAGFVEGVRRTRATE----MTQMSVFWLALPLILMGLC 462

Query: 364 EAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQWL 423
           E+F  I ++E   +  PE +R++A ++F LS + A+YLSS+L+  V K+   +     WL
Sbjct: 463 ESFNFIGLIEFFNSQFPEHMRSIANSLFPLSFAAANYLSSLLVTTVHKVSGTK-DHPDWL 522

Query: 424 GGNDLNKNRLDYFFYVVGGIAALNFFYFRFFA 447
              DL++ +LDYF+Y++  +  +N  YF + A
Sbjct: 523 -NKDLDRGKLDYFYYLIAVLGVVNLVYFWYCA 542

BLAST of Bhi03G001607 vs. TAIR10
Match: AT5G62680.1 (Major facilitator superfamily protein)

HSP 1 Score: 304.3 bits (778), Expect = 1.3e-82
Identity = 168/455 (36.92%), Postives = 272/455 (59.78%), Query Frame = 0

Query: 3   VLSLGAGLPQMRPPPC-ISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGADQ 62
           V+ L A +PQ+ P  C  + +S C  P   Q+ FL +GLG +VVGAGGIRPCN++FGADQ
Sbjct: 131 VILLTAAVPQLHPAACGTAADSICNGPSGGQIAFLLMGLGFLVVGAGGIRPCNLAFGADQ 190

Query: 63  FDTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSILI 122
           F+  +E G+  ++SF NW++  F+ A +++LT+VVYVQ+N+SWT+G  IP     ++ LI
Sbjct: 191 FNPKSESGKRGIDSFFNWYFFTFTFAQILSLTLVVYVQSNVSWTIGLTIPAVLMFLACLI 250

Query: 123 FLSGRRFYVCKAPQGSVFSDLAKVVVAMFRKRKIPKPNQ----IDHLHNPPMNSCKLAHT 182
           F +G + YV     GS  + +A+V+    +KR +    Q    + + + P   + KL +T
Sbjct: 251 FFAGDKLYVKIKASGSPLAGIAQVIAVAIKKRGLKPAKQPWLNLYNYYPPKYANSKLKYT 310

Query: 183 DRFLVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFISM 242
           D+F   DKAA ++    +L  +GK  D W+LC++ QVE+ KC+V ++P+W A    ++++
Sbjct: 311 DQFRFLDKAA-ILTPEDKLQPDGKPADPWKLCTMQQVEEVKCIVRVLPIWFASSIYYLTI 370

Query: 243 QQMGSFGILQAIQMNRFIGP-HFQIPPAWMSLTPMIALSIWIYIYEK-YVEFMKKKTHSN 302
            Q  ++ + QA+Q +R +G   F IP A   +  M  ++++I +Y++  V  M++ T  +
Sbjct: 371 TQQMTYPVFQALQSDRRLGSGGFVIPAATYVVFLMTGMTVFIVVYDRVLVPTMRRITGLD 430

Query: 303 KRFSMKTRIEIGIVMSVLCMVVAGLLEKLRRDAAV--------ENKSFVSPLHVWMLIPE 362
              ++  RI  GI  +   +VVAG +E+ RR  A+          K  +S +    LIP+
Sbjct: 431 TGITLLQRIGTGIFFATASLVVAGFVEERRRTFALTKPTLGMAPRKGEISSMSAMWLIPQ 490

Query: 363 FALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRME 422
            +L+G+ EAFAAI  ME      PE++R+ AG+IF++   ++SYL S L+  V +  +  
Sbjct: 491 LSLAGVAEAFAAIGQMEFYYKQFPENMRSFAGSIFYVGGGVSSYLGSFLIATVHRTTQNS 550

Query: 423 AGEMQWLGGNDLNKNRLDYFFYVVGGIAALNFFYF 443
           +G   WL   DLNK RLD F++++ GI A+NF YF
Sbjct: 551 SGG-NWL-AEDLNKGRLDLFYFMIAGILAVNFAYF 582

BLAST of Bhi03G001607 vs. TAIR10
Match: AT3G47960.1 (Major facilitator superfamily protein)

HSP 1 Score: 289.7 bits (740), Expect = 3.4e-78
Identity = 162/459 (35.29%), Postives = 270/459 (58.82%), Query Frame = 0

Query: 3   VLSLGAGLPQMRPPPCISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGADQF 62
           V+ L A +P + P  C   + SC  P   Q+ FL +GLG +VVGAGGIRPCN++FGADQF
Sbjct: 149 VILLTAAIPSLHPVAC-GNKISCEGPSVGQILFLLMGLGFLVVGAGGIRPCNLAFGADQF 208

Query: 63  DTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSILIF 122
           +  +E G+  + SF NW++  F+ A +I+LT VVY+Q+N+SWT+G  IP A   ++ +IF
Sbjct: 209 NPKSESGKKGINSFFNWYFFTFTFAQIISLTAVVYIQSNVSWTIGLIIPVALMFLACVIF 268

Query: 123 LSGRRFYVCKAPQGSVFSDLAKVVVAMFRKR-----KIPKPNQIDHLHNPPMNSCKLAHT 182
            +G R YV     GS  + +A+V+ A  +KR     K P  N  +H+ +   N+  L +T
Sbjct: 269 FAGDRLYVKVKASGSPLAGIARVIAAAIKKRGLKPVKQPWVNLYNHIPSNYANT-TLKYT 328

Query: 183 DRFLVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFISM 242
           D+F   DKAA ++    +L+ +G + D W+LC++ QVE+ KC+V +IP+W A    ++++
Sbjct: 329 DQFRFLDKAA-IMTPEEKLNSDGTASDPWKLCTLQQVEEVKCIVRVIPIWFASTIYYLAI 388

Query: 243 QQMGSFGILQAIQMNRFIGP-HFQIPPAWMSLTPMIALSIWIYIYEK-YVEFMKKKTHSN 302
               ++ + QA+Q +R +G   F+IP A   +  M  ++++I  Y++  V  +++ T   
Sbjct: 389 TIQMTYPVFQALQSDRRLGSGGFRIPAATYVVFLMTGMTVFIIFYDRVLVPSLRRVTGLE 448

Query: 303 KRFSMKTRIEIGIVMSVLCMVVAGLLEKLRRDAAVENKSF--------VSPLHVWMLIPE 362
              S+  RI  G   +++ ++V+G +E+ RR+ A+   +         +S +    LIP+
Sbjct: 449 TGISLLQRIGAGFTFAIMSLLVSGFIEERRRNFALTKPTLGMAPRTGEISSMSALWLIPQ 508

Query: 363 FALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRME 422
             L+G+ EAFAAI  ME      PE++++ AG+IF++   ++SYL+S L+  V +     
Sbjct: 509 LTLAGIAEAFAAIGQMEFYYKQFPENMKSFAGSIFYVGAGVSSYLASFLISTVHRT-TAH 568

Query: 423 AGEMQWLGGNDLNKNRLDYFFYVVGGIAALNFFYFRFFA 447
           +    WL   DLNK +LDYF++++ G+  +N  YF   A
Sbjct: 569 SPSGNWL-AEDLNKAKLDYFYFMLTGLMVVNMAYFLLMA 602

BLAST of Bhi03G001607 vs. TrEMBL
Match: tr|A0A1S4DX14|A0A1S4DX14_CUCME (protein NRT1/ PTR FAMILY 2.8-like OS=Cucumis melo OX=3656 GN=LOC103491761 PE=4 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 1.8e-243
Identity = 422/474 (89.03%), Postives = 444/474 (93.67%), Query Frame = 0

Query: 1   MGVLSLGAGLPQMRPPPCISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGAD 60
           MGVL+LGAGLPQMRPPPCISGES+CPQP PWQL FLYLGLGLIV+GAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGLPQMRPPPCISGESNCPQPLPWQLSFLYLGLGLIVIGAGGIRPCNISFGAD 60

Query: 61  QFDTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSIL 120
           QFDTNTEKGRAKLESFLNWWY LFS+ALVIALTIVVYVQTN+SWTLGF IPTACFV SI 
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYFLFSVALVIALTIVVYVQTNVSWTLGFGIPTACFVFSIS 120

Query: 121 IFLSGRRFYVCKAPQGSVFSDLAKVVVAMFRKRKIPKPNQIDHLHNPPMNSCKLAHTDRF 180
           IFL G R+Y+CKAPQGSVF+D+AKVVVA  RKR+IPKP Q D LHNPPMNS KLAHT+RF
Sbjct: 121 IFLLGHRYYICKAPQGSVFADIAKVVVATCRKRQIPKPTQFDQLHNPPMNSSKLAHTNRF 180

Query: 181 LVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFISMQQM 240
           L+FDKAATVVDSS+ELDE+GKSK+EWRLCSVHQVE+FKCVVGIIPVWLAGI CF+SMQQM
Sbjct: 181 LIFDKAATVVDSSVELDEDGKSKNEWRLCSVHQVEEFKCVVGIIPVWLAGIPCFMSMQQM 240

Query: 241 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHSNKRFSM 300
           GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMI LSIWIYIYEKYVE MKKKT SNKRFSM
Sbjct: 241 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMITLSIWIYIYEKYVEHMKKKTRSNKRFSM 300

Query: 301 KTRIEIGIVMSVLCMVVAGLLEKLRRDAAVENKSFVSPLHVWMLIPEFALSGLTEAFAAI 360
           KTRIEIGIVMSVLCMVVAG+LEKLRRDAAVENKSF+SPLHVW+LIPEFALSGLTEAFAAI
Sbjct: 301 KTRIEIGIVMSVLCMVVAGILEKLRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFAAI 360

Query: 361 AVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQWLGGNDLN 420
           AVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVL GIVSK+      E +WLGGNDLN
Sbjct: 361 AVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLTGIVSKV------EREWLGGNDLN 420

Query: 421 KNRLDYFFYVVGGIAALNFFYFRFFAAGFLPSPDVDPRQKAHQRQLEDAELGRR 475
           KNRLDYFFYVVG IAALNFFYFRFFA  FLP PDVDPRQKAHQRQLEDAELGRR
Sbjct: 421 KNRLDYFFYVVGVIAALNFFYFRFFAGAFLPKPDVDPRQKAHQRQLEDAELGRR 468

BLAST of Bhi03G001607 vs. TrEMBL
Match: tr|A0A0A0KZQ3|A0A0A0KZQ3_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G297490 PE=4 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 4.2e-168
Identity = 322/465 (69.25%), Postives = 337/465 (72.47%), Query Frame = 0

Query: 1   MGVLSLGAGLPQMRPPPCISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGAD 60
           MGVL+LGAGLPQMRPPPCISGES+CPQP PWQLGFLYLGLGLIV+GAG            
Sbjct: 105 MGVLTLGAGLPQMRPPPCISGESNCPQPLPWQLGFLYLGLGLIVIGAG------------ 164

Query: 61  QFDTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSIL 120
                                                                       
Sbjct: 165 ------------------------------------------------------------ 224

Query: 121 IFLSGRRFYVCKAPQGSVFSDLAKVVVAMFRKRKIPKPNQIDHLHNPPMNSCKLAHTDRF 180
                               D+AKVVVA  RK +IPK  Q D LHNPPMNS KLAHT+RF
Sbjct: 225 --------------------DMAKVVVATCRKWQIPKTTQFDQLHNPPMNSSKLAHTNRF 284

Query: 181 LVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFISMQQM 240
           L+FDKAATVVDSS+ELDEEGKSK+EWRLCSVHQVEQFKCVVGI PVWLAGI CF+SMQQM
Sbjct: 285 LIFDKAATVVDSSVELDEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQM 344

Query: 241 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHSNKRFSM 300
            SFGILQAIQMNR IGPHFQIPPAWMSLTPMIALSIWIYIYEKYVE MKKKT SNKRFSM
Sbjct: 345 SSFGILQAIQMNRSIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSM 404

Query: 301 KTRIEIGIVMSVLCMVVAGLLEKLRRDAAVENKSFVSPLHVWMLIPEFALSGLTEAFAAI 360
           KTRIEIGIVMSVLCMVVAG+LEK RRDAAVENKSF+SPLHVW+LIPEFALSGLTEAF AI
Sbjct: 405 KTRIEIGIVMSVLCMVVAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAI 464

Query: 361 AVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQWLGGNDLN 420
           AVMELLTTHLPESLRTVAGAIFF SLSLASYLSSVL GIV  +      E +WLGGNDLN
Sbjct: 465 AVMELLTTHLPESLRTVAGAIFFFSLSLASYLSSVLTGIVRGV------EREWLGGNDLN 471

Query: 421 KNRLDYFFYVVGGIAALNFFYFRFFAAGFLPSPDVDPRQKAHQRQ 466
           KNRLDYFF VVG IAALNFFYFRFFA  FLP PDVDP  KAHQ Q
Sbjct: 525 KNRLDYFFSVVGVIAALNFFYFRFFAVAFLPKPDVDPSHKAHQMQ 471

BLAST of Bhi03G001607 vs. TrEMBL
Match: tr|A0A1Q3BBQ1|A0A1Q3BBQ1_CEPFO (PTR2 domain-containing protein (Fragment) OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_08956 PE=4 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 2.9e-148
Identity = 264/456 (57.89%), Postives = 349/456 (76.54%), Query Frame = 0

Query: 1   MGVLSLGAGLPQMRPPPCISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGAD 60
           M  ++L AG+PQ+RP  C + ES CP PQ WQLGFL  GL L+ +GAGGIRPCNI+FGAD
Sbjct: 75  MATMTLTAGVPQLRPTTC-NEESDCPGPQKWQLGFLLSGLALLAIGAGGIRPCNIAFGAD 134

Query: 61  QFDTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSIL 120
           QFDT T+KGRA+LESF NWWY  F++ALV+ALT+VVY+QTN+SW LGFAIPTAC V+SI 
Sbjct: 135 QFDTTTKKGRAQLESFFNWWYFSFTLALVVALTVVVYIQTNVSWVLGFAIPTACLVLSIG 194

Query: 121 IFLSGRRFYVCKAPQGSVFSDLAKVVVAMFRKRKIPKPNQIDHLHNPPM-----NSCKLA 180
           IFL G+  Y+   PQGSVF D+AKV+ A FRKR +   +  + L++PP+      + KL 
Sbjct: 195 IFLIGKNTYIILKPQGSVFVDMAKVITAAFRKRNVDVRSASEQLYDPPIKESDSQTTKLT 254

Query: 181 HTDRFLVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFI 240
           HT++F   DKAA   DSS ELD+EGK K+ WRLC++ QVEQ K ++G++PVWL GI CF+
Sbjct: 255 HTNKFRCLDKAAINTDSS-ELDDEGKPKNGWRLCTLQQVEQLKLLLGMVPVWLTGIGCFV 314

Query: 241 SMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEK-YVEFMKKKTHS 300
           +M Q  + GILQAIQMN+ +G HF+IPP WM L+ MIALSIWI+IYE+ Y+   KK T  
Sbjct: 315 AMDQQNTIGILQAIQMNKSVGSHFKIPPGWMGLSSMIALSIWIFIYERIYIPRAKKMTGK 374

Query: 301 NKRFSMKTRIEIGIVMSVLCMVVAGLLEKLRRDAAVENKSFVSPLHVWMLIPEFALSGLT 360
           +KR +M  RI IGIVM++LC +VAG++EK RR+AA+ N SF SPL + +L+P+FALSGL 
Sbjct: 375 DKRMTMTQRINIGIVMAILCSLVAGIVEKHRREAALRNGSFESPLSITLLLPQFALSGLI 434

Query: 361 EAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQWL 420
           EAFAA+A+ME LTT LPE++RTV+GAIFFLSLS ASYL+S+ + I+  +  ++ G+  WL
Sbjct: 435 EAFAAVAIMEFLTTQLPETMRTVSGAIFFLSLSAASYLNSLFVNIIYSL-TLKNGK-PWL 494

Query: 421 GGNDLNKNRLDYFFYVVGGIAALNFFYFRFFAAGFL 451
           GG DLNK+ L+YF++++ G+  +NF YF FFA+ ++
Sbjct: 495 GGKDLNKDTLEYFYFLIAGLGVVNFTYFNFFASRYV 526

BLAST of Bhi03G001607 vs. TrEMBL
Match: tr|A0A2C9VRC4|A0A2C9VRC4_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_06G089900 PE=4 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.1e-147
Identity = 259/478 (54.18%), Postives = 353/478 (73.85%), Query Frame = 0

Query: 1   MGVLSLGAGLPQMRPPPCISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGAD 60
           M  ++L A +PQ+ PP C  GES C QP+ WQLGFL+  LG + +GAGGIRPCNI+FGAD
Sbjct: 109 MVAMTLTAAVPQLTPPKC-EGESHCLQPEKWQLGFLFTSLGFLSIGAGGIRPCNIAFGAD 168

Query: 61  QFDTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSIL 120
           QFDT T KGRA LESF NWWY  F+IAL++ALT V+Y+QT++SW +G  IPTAC  +S+ 
Sbjct: 169 QFDTRTAKGRANLESFFNWWYFFFTIALLVALTGVIYIQTDVSWVIGLLIPTACLCLSLS 228

Query: 121 IFLSGRRFYVCKAPQGSVFSDLAKVVVAMFRKRKIP-KPNQIDHLHNPPMNS----CKLA 180
           IFL GR  Y+CK PQGS+F+D+ KV+ A +RKR++   P      ++PPMN      KL+
Sbjct: 229 IFLIGRHTYICKKPQGSIFADMLKVITAAYRKRQLNIVPGSQHSFYDPPMNDSDLHLKLS 288

Query: 181 HTDRFLVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFI 240
            TD F + DKAA + D S ELD +G+ K+ WRLCS+HQVEQ KC+V I+PVW++GIACFI
Sbjct: 289 PTDGFKLLDKAAVITDPS-ELDAQGRPKNTWRLCSLHQVEQLKCLVAILPVWVSGIACFI 348

Query: 241 SMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYE-KYVEFMKKKTHS 300
            M Q  +FGILQAIQMN+ +G H  IPP WM LT MIALS+W++IYE  ++   KK T  
Sbjct: 349 PMDQQNAFGILQAIQMNKSLGSHLTIPPGWMGLTSMIALSMWVFIYEFIFIRLAKKLTGR 408

Query: 301 NKRFSMKTRIEIGIVMSVLCMVVAGLLEKLRRDAAVENKSFVSPLHVWMLIPEFALSGLT 360
           +KR ++  +I IGIV+S+LCM+VA ++EK RRD+A+++ SF SP+ V +L+P+F LSGL 
Sbjct: 409 DKRLTVAQKITIGIVLSILCMLVAAIVEKQRRDSALKHGSFASPITVGLLLPQFILSGLV 468

Query: 361 EAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQWL 420
           EAFAA+++ME  TT +PES+RTVAGA+FFLSLS+ASY+ S+++ I+  +   + G+  WL
Sbjct: 469 EAFAAVSIMEFYTTQMPESMRTVAGAVFFLSLSIASYVGSLIINIIHHV-TGKNGKSPWL 528

Query: 421 GGNDLNKNRLDYFFYVVGGIAALNFFYFRFFAAGFLPSPDVDPRQKAHQRQLEDAELG 473
           GG+DLNKNRLDY++Y++ G+ A+NF YF FFA+ +  + +++     H R+L   E G
Sbjct: 529 GGHDLNKNRLDYYYYIIAGLGAVNFVYFSFFASRY--AVELEKSSNCHSRKLHGDETG 581

BLAST of Bhi03G001607 vs. TrEMBL
Match: tr|A0A059CZ68|A0A059CZ68_EUCGR (Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_B00403 PE=4 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 2.4e-147
Identity = 263/458 (57.42%), Postives = 352/458 (76.86%), Query Frame = 0

Query: 1   MGVLSLGAGLPQMRPPPCISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGAD 60
           M  ++L AG+P++ P  CI G+S+CPQPQ WQL FL+ GL L+ +GAGGIRPCNI+FGAD
Sbjct: 135 MLTMTLTAGIPRLHPSKCI-GKSNCPQPQSWQLCFLFAGLALLAIGAGGIRPCNIAFGAD 194

Query: 61  QFDTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSIL 120
           QFDT T++GRA+LESF NWWY  F++AL+IALT V+YVQTN+SW LGFAIPTAC  +SI 
Sbjct: 195 QFDTRTDEGRAQLESFFNWWYFSFTVALLIALTAVIYVQTNVSWVLGFAIPTACLGLSIS 254

Query: 121 IFLSGRRFYVCKAPQGSVFSDLAKVVV-AMFRKRKIPKPNQIDHLHNP-PMNS----CKL 180
           IFL GR+ Y+CK PQGS+F D+AKV+V A +++R+   P++    ++P P  S     KL
Sbjct: 255 IFLLGRKTYICKKPQGSIFVDMAKVLVAACWKRRQTLGPDREQPSYDPSPEESDQGVVKL 314

Query: 181 AHTDRFLVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACF 240
             T RF   DKAA ++D + ELD +G SK+ WRLCSV QVEQFKC++GI+PVW++GIACF
Sbjct: 315 TRTYRFRFLDKAALIIDPN-ELDSQGMSKNGWRLCSVQQVEQFKCLIGILPVWISGIACF 374

Query: 241 ISMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEK-YVEFMKKKTH 300
           I+M Q  +FGILQAIQMN+ IGPHF+IPPAWM+L  MIALSIWI+IYE+ Y+  +++   
Sbjct: 375 IAMDQQNTFGILQAIQMNKSIGPHFEIPPAWMTLFSMIALSIWIFIYERIYIPHVRRIIK 434

Query: 301 SNKRFSMKTRIEIGIVMSVLCMVVAGLLEKLRRDAAVENKSFVSPLHVWMLIPEFALSGL 360
            +KR +M+ RI  GI+MS+LCM+VAG++E+ RRD+A+   S+ SP+ V +L+P+F LSG+
Sbjct: 435 KDKRLTMQQRISTGIIMSILCMLVAGIVERKRRDSALSRGSYESPITVALLLPQFVLSGM 494

Query: 361 TEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQW 420
            EAFAA+A++E LTT +PES+RTVAGAIFFLSLS+ASY+ S L+ ++  I R       W
Sbjct: 495 VEAFAAVALLEFLTTQMPESMRTVAGAIFFLSLSIASYIGSFLVNMIHIITRKNRNS-PW 554

Query: 421 LGGNDLNKNRLDYFFYVVGGIAALNFFYFRFFAAGFLP 452
           LGG+DLNKNRL+ ++Y++  + A NF YF FFA  + P
Sbjct: 555 LGGHDLNKNRLENYYYIIAAVGAANFVYFNFFACHYHP 589

BLAST of Bhi03G001607 vs. NCBI nr
Match: XP_016900519.1 (PREDICTED: protein NRT1/ PTR FAMILY 2.8-like [Cucumis melo])

HSP 1 Score: 850.5 bits (2196), Expect = 2.7e-243
Identity = 422/474 (89.03%), Postives = 444/474 (93.67%), Query Frame = 0

Query: 1   MGVLSLGAGLPQMRPPPCISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGAD 60
           MGVL+LGAGLPQMRPPPCISGES+CPQP PWQL FLYLGLGLIV+GAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGLPQMRPPPCISGESNCPQPLPWQLSFLYLGLGLIVIGAGGIRPCNISFGAD 60

Query: 61  QFDTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSIL 120
           QFDTNTEKGRAKLESFLNWWY LFS+ALVIALTIVVYVQTN+SWTLGF IPTACFV SI 
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYFLFSVALVIALTIVVYVQTNVSWTLGFGIPTACFVFSIS 120

Query: 121 IFLSGRRFYVCKAPQGSVFSDLAKVVVAMFRKRKIPKPNQIDHLHNPPMNSCKLAHTDRF 180
           IFL G R+Y+CKAPQGSVF+D+AKVVVA  RKR+IPKP Q D LHNPPMNS KLAHT+RF
Sbjct: 121 IFLLGHRYYICKAPQGSVFADIAKVVVATCRKRQIPKPTQFDQLHNPPMNSSKLAHTNRF 180

Query: 181 LVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFISMQQM 240
           L+FDKAATVVDSS+ELDE+GKSK+EWRLCSVHQVE+FKCVVGIIPVWLAGI CF+SMQQM
Sbjct: 181 LIFDKAATVVDSSVELDEDGKSKNEWRLCSVHQVEEFKCVVGIIPVWLAGIPCFMSMQQM 240

Query: 241 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHSNKRFSM 300
           GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMI LSIWIYIYEKYVE MKKKT SNKRFSM
Sbjct: 241 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMITLSIWIYIYEKYVEHMKKKTRSNKRFSM 300

Query: 301 KTRIEIGIVMSVLCMVVAGLLEKLRRDAAVENKSFVSPLHVWMLIPEFALSGLTEAFAAI 360
           KTRIEIGIVMSVLCMVVAG+LEKLRRDAAVENKSF+SPLHVW+LIPEFALSGLTEAFAAI
Sbjct: 301 KTRIEIGIVMSVLCMVVAGILEKLRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFAAI 360

Query: 361 AVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQWLGGNDLN 420
           AVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVL GIVSK+      E +WLGGNDLN
Sbjct: 361 AVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLTGIVSKV------EREWLGGNDLN 420

Query: 421 KNRLDYFFYVVGGIAALNFFYFRFFAAGFLPSPDVDPRQKAHQRQLEDAELGRR 475
           KNRLDYFFYVVG IAALNFFYFRFFA  FLP PDVDPRQKAHQRQLEDAELGRR
Sbjct: 421 KNRLDYFFYVVGVIAALNFFYFRFFAGAFLPKPDVDPRQKAHQRQLEDAELGRR 468

BLAST of Bhi03G001607 vs. NCBI nr
Match: XP_004142186.1 (PREDICTED: protein NRT1/ PTR FAMILY 2.8-like [Cucumis sativus])

HSP 1 Score: 824.3 bits (2128), Expect = 2.1e-235
Identity = 413/474 (87.13%), Postives = 432/474 (91.14%), Query Frame = 0

Query: 1   MGVLSLGAGLPQMRPPPCISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGAD 60
           MGVL+LGAGLPQMRPPPCISGES+CPQP PWQLGFLYLGLGLIV+GAGGIRPCNISFGAD
Sbjct: 105 MGVLTLGAGLPQMRPPPCISGESNCPQPLPWQLGFLYLGLGLIVIGAGGIRPCNISFGAD 164

Query: 61  QFDTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSIL 120
           QFDTNTEKGRAKLESFLNWWY LFSI+LVIALTIVVYVQTNISWTLGF IPTACFV SI 
Sbjct: 165 QFDTNTEKGRAKLESFLNWWYFLFSISLVIALTIVVYVQTNISWTLGFGIPTACFVFSIF 224

Query: 121 IFLSGRRFYVCKAPQGSVFSDLAKVVVAMFRKRKIPKPNQIDHLHNPPMNSCKLAHTDRF 180
           IFL G R+Y+CKAPQGSVF+D+AKVVVA  RK +IPK  Q D LHNPPMNS KLAHT+RF
Sbjct: 225 IFLLGHRYYICKAPQGSVFADMAKVVVATCRKWQIPKTTQFDQLHNPPMNSSKLAHTNRF 284

Query: 181 LVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFISMQQM 240
           L+FDKAATVVDSS+ELDEEGKSK+EWRLCSVHQVEQFKCVVGI PVWLAGI CF+SMQQM
Sbjct: 285 LIFDKAATVVDSSVELDEEGKSKNEWRLCSVHQVEQFKCVVGIFPVWLAGIPCFMSMQQM 344

Query: 241 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHSNKRFSM 300
            SFGILQAIQMNR IGPHFQIPPAWMSLTPMIALSIWIYIYEKYVE MKKKT SNKRFSM
Sbjct: 345 SSFGILQAIQMNRSIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEHMKKKTQSNKRFSM 404

Query: 301 KTRIEIGIVMSVLCMVVAGLLEKLRRDAAVENKSFVSPLHVWMLIPEFALSGLTEAFAAI 360
           KTRIEIGIVMSVLCMVVAG+LEK RRDAAVENKSF+SPLHVW+LIPEFALSGLTEAF AI
Sbjct: 405 KTRIEIGIVMSVLCMVVAGVLEKFRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFGAI 464

Query: 361 AVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQWLGGNDLN 420
           AVMELLTTHLPESLRTVAGAIFF SLSLASYLSSVL GIV  +      E +WLGGNDLN
Sbjct: 465 AVMELLTTHLPESLRTVAGAIFFFSLSLASYLSSVLTGIVRGV------EREWLGGNDLN 524

Query: 421 KNRLDYFFYVVGGIAALNFFYFRFFAAGFLPSPDVDPRQKAHQRQLEDAELGRR 475
           KNRLDYFF VVG IAALNFFYFRFFA  FLP PDVDP  KAHQ QLEDAELGRR
Sbjct: 525 KNRLDYFFSVVGVIAALNFFYFRFFAVAFLPKPDVDPSHKAHQMQLEDAELGRR 572

BLAST of Bhi03G001607 vs. NCBI nr
Match: XP_023544027.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 805.1 bits (2078), Expect = 1.3e-229
Identity = 393/455 (86.37%), Postives = 425/455 (93.41%), Query Frame = 0

Query: 1   MGVLSLGAGLPQMRPPPCISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGAD 60
           MGVL+LGAG P+MRPPPC  GES+CPQP PWQLGFLYLGLGL+V+GAGGIRPCNISFGAD
Sbjct: 102 MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD 161

Query: 61  QFDTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSIL 120
           QFDTNTEKGRAKLESFLNWWYLLFS+ALVIALT+VVYVQT+ISWTLGFAIPTACFV SIL
Sbjct: 162 QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYVQTSISWTLGFAIPTACFVFSIL 221

Query: 121 IFLSGRRFYVCKAPQGSVFSDLAKVVVAMFRKRKIPKPNQIDHLHNPPMNSCKLAHTDRF 180
           IFL GRRFY+CK PQGSVF+D+AKV+VA  RKR +PK  QIDHLH+PPM+S KL+HTDRF
Sbjct: 222 IFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQIDHLHDPPMDSSKLSHTDRF 281

Query: 181 LVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFISMQQM 240
           LVFDKAATVVDSS ELDE+GKSK+EW+LCSVHQVEQFKCVVGI+P+W+AGI CFISMQQM
Sbjct: 282 LVFDKAATVVDSSTELDEQGKSKNEWKLCSVHQVEQFKCVVGIVPIWVAGIPCFISMQQM 341

Query: 241 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHSNKRFSM 300
           GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEK+VEFMKKK H NKRFSM
Sbjct: 342 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKFVEFMKKKNHKNKRFSM 401

Query: 301 KTRIEIGIVMSVLCMVVAGLLEKLRRDAAVENKSFVSPLHVWMLIPEFALSGLTEAFAAI 360
           KTRIEIGIVMSV+CMVVAG+LE +RR++AVENKSFVSPLHVWMLIPEFALSGLTEAFAAI
Sbjct: 402 KTRIEIGIVMSVICMVVAGILENVRRNSAVENKSFVSPLHVWMLIPEFALSGLTEAFAAI 461

Query: 361 AVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQWLGGNDLN 420
           AVMELLTTHLPESLRTVAGAIFFLSLSL SY+SSVLM IVSKI R+E G+  WLGGNDLN
Sbjct: 462 AVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSGWLGGNDLN 521

Query: 421 KNRLDYFFYVVGGIAALNFFYFRFFAAGFLPSPDV 456
           KNRLDYFFYVVG IAA NF YFRFFA  FLP P+V
Sbjct: 522 KNRLDYFFYVVGVIAAFNFLYFRFFATHFLPKPNV 556

BLAST of Bhi03G001607 vs. NCBI nr
Match: XP_022950593.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucurbita moschata])

HSP 1 Score: 802.7 bits (2072), Expect = 6.5e-229
Identity = 395/456 (86.62%), Postives = 424/456 (92.98%), Query Frame = 0

Query: 1   MGVLSLGAGLPQMRPPPCISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGAD 60
           MGVL+LGAG P+MRPPPC  GES+CPQP PWQLGFLYLGLGL+V+GAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD 60

Query: 61  QFDTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSIL 120
           QFDTNTEKGRAKLESFLNWWYLLFS+ALVIALT+VVYVQT+ISWTLGFAIPTACFV SIL
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYVQTSISWTLGFAIPTACFVFSIL 120

Query: 121 IFLSGRRFYVCKAPQGSVFSDLAKVVVAMFRKRKIPKPNQIDH-LHNPPMNSCKLAHTDR 180
           IFL GRRFY+CK PQGSVF+D+AKV+VA  RKR +PK  QIDH LH+PPM+S KL+HTDR
Sbjct: 121 IFLLGRRFYICKTPQGSVFADIAKVLVATCRKRNVPKFTQIDHFLHDPPMDSSKLSHTDR 180

Query: 181 FLVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFISMQQ 240
           FLVFDKAATVVDSS ELDE+GKSK+EW+LCSVHQVEQFKCVVGI+P+W AGI CFISMQQ
Sbjct: 181 FLVFDKAATVVDSSTELDEQGKSKNEWKLCSVHQVEQFKCVVGIVPIWAAGIPCFISMQQ 240

Query: 241 MGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHSNKRFS 300
           MGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTH NKRFS
Sbjct: 241 MGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKRFS 300

Query: 301 MKTRIEIGIVMSVLCMVVAGLLEKLRRDAAVENKSFVSPLHVWMLIPEFALSGLTEAFAA 360
           +KTRIEIGIVMSVLCMVVAG+LE +RR +A ENKSFVSPLHVWMLIPEFALSGLTEAFAA
Sbjct: 301 LKTRIEIGIVMSVLCMVVAGILENIRRSSAEENKSFVSPLHVWMLIPEFALSGLTEAFAA 360

Query: 361 IAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQWLGGNDL 420
           IAVMELLTTHLPESLRTVAGAIFFLSLSL SY+SSVLM IVSKI R+E G+  WLGGNDL
Sbjct: 361 IAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSGWLGGNDL 420

Query: 421 NKNRLDYFFYVVGGIAALNFFYFRFFAAGFLPSPDV 456
           NKNRLDYFFYVVG IAA NFFYFRFFA  FLP P+V
Sbjct: 421 NKNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 456

BLAST of Bhi03G001607 vs. NCBI nr
Match: XP_022978168.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucurbita maxima])

HSP 1 Score: 799.3 bits (2063), Expect = 7.2e-228
Identity = 391/455 (85.93%), Postives = 422/455 (92.75%), Query Frame = 0

Query: 1   MGVLSLGAGLPQMRPPPCISGESSCPQPQPWQLGFLYLGLGLIVVGAGGIRPCNISFGAD 60
           MGVL+LGAG P+MRPPPC  GES+CPQP PWQLGFLYLGLGL+V+GAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD 60

Query: 61  QFDTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTNISWTLGFAIPTACFVVSIL 120
           QFDTNTEKGRAKLESFLNWWYLLFS+ALVIALT+VVY+QT+ISWTLGFAIPTACFV SIL
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTSISWTLGFAIPTACFVFSIL 120

Query: 121 IFLSGRRFYVCKAPQGSVFSDLAKVVVAMFRKRKIPKPNQIDHLHNPPMNSCKLAHTDRF 180
           IFL GRRFY+CK PQGSVF+D+AKV+VA  RKR +PK  QIDHL++PP++S KLAHTDRF
Sbjct: 121 IFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQIDHLYDPPIDSSKLAHTDRF 180

Query: 181 LVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVVGIIPVWLAGIACFISMQQM 240
           LVFDKAATV DSS ELDE+GKSK+EW+LCSVHQVEQ KCVVGI+P+W AGI CFISMQQM
Sbjct: 181 LVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLKCVVGIVPIWAAGIPCFISMQQM 240

Query: 241 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHSNKRFSM 300
           GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTH NKRFSM
Sbjct: 241 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKRFSM 300

Query: 301 KTRIEIGIVMSVLCMVVAGLLEKLRRDAAVENKSFVSPLHVWMLIPEFALSGLTEAFAAI 360
           KTRIEIGIVMSVLCMVVAG+LE +RR +AVENK FVSPLHVW LIPEFALSGLTEAFAAI
Sbjct: 301 KTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFVSPLHVWTLIPEFALSGLTEAFAAI 360

Query: 361 AVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVSKIGRMEAGEMQWLGGNDLN 420
           AVMELLTTHLPESLRTVAGAIFFLSLSL SY+SSVLM IVSKI R+E G+ +WLGGNDLN
Sbjct: 361 AVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSEWLGGNDLN 420

Query: 421 KNRLDYFFYVVGGIAALNFFYFRFFAAGFLPSPDV 456
           KNRLDYFFYVVG IAA NFFYFRFFA  FLP P+V
Sbjct: 421 KNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 455

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|Q3E8X3|PTR52_ARATH3.1e-12951.75Protein NRT1/ PTR FAMILY 2.8 OS=Arabidopsis thaliana OX=3702 GN=NPF2.8 PE=2 SV=2[more]
sp|Q8RX77|PTR21_ARATH1.5e-8838.38Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV... [more]
sp|Q9LFX9|PTR13_ARATH3.2e-8639.60Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV... [more]
sp|Q9LV10|PTR53_ARATH2.4e-8136.92Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV... [more]
sp|Q944G5|PTR44_ARATH6.1e-7735.29Protein NRT1/ PTR FAMILY 2.10 OS=Arabidopsis thaliana OX=3702 GN=NPF2.10 PE=1 SV... [more]
Match NameE-valueIdentityDescription
AT5G28470.11.7e-13051.75Major facilitator superfamily protein[more]
AT1G69870.18.5e-9038.38nitrate transporter 1.7[more]
AT1G27080.11.8e-8739.60nitrate transporter 1.6[more]
AT5G62680.11.3e-8236.92Major facilitator superfamily protein[more]
AT3G47960.13.4e-7835.29Major facilitator superfamily protein[more]
Match NameE-valueIdentityDescription
tr|A0A1S4DX14|A0A1S4DX14_CUCME1.8e-24389.03protein NRT1/ PTR FAMILY 2.8-like OS=Cucumis melo OX=3656 GN=LOC103491761 PE=4 S... [more]
tr|A0A0A0KZQ3|A0A0A0KZQ3_CUCSA4.2e-16869.25Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G297490 PE=4 SV=1[more]
tr|A0A1Q3BBQ1|A0A1Q3BBQ1_CEPFO2.9e-14857.89PTR2 domain-containing protein (Fragment) OS=Cephalotus follicularis OX=3775 GN=... [more]
tr|A0A2C9VRC4|A0A2C9VRC4_MANES1.1e-14754.18Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_06G089900 PE=4 SV=... [more]
tr|A0A059CZ68|A0A059CZ68_EUCGR2.4e-14757.42Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_B00403 PE=4 SV... [more]
Match NameE-valueIdentityDescription
XP_016900519.12.7e-24389.03PREDICTED: protein NRT1/ PTR FAMILY 2.8-like [Cucumis melo][more]
XP_004142186.12.1e-23587.13PREDICTED: protein NRT1/ PTR FAMILY 2.8-like [Cucumis sativus][more]
XP_023544027.11.3e-22986.37protein NRT1/ PTR FAMILY 2.8-like [Cucurbita pepo subsp. pepo][more]
XP_022950593.16.5e-22986.62protein NRT1/ PTR FAMILY 2.8-like [Cucurbita moschata][more]
XP_022978168.17.2e-22885.93protein NRT1/ PTR FAMILY 2.8-like [Cucurbita maxima][more]
The following terms have been associated with this gene:
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
Vocabulary: Biological Process
TermDefinition
GO:0055085transmembrane transport
Vocabulary: Molecular Function
TermDefinition
GO:0022857transmembrane transporter activity
Vocabulary: INTERPRO
TermDefinition
IPR036259MFS_trans_sf
IPR000109POT_fam
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042886 amide transport
biological_process GO:0055085 transmembrane transport
biological_process GO:0006810 transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0035673 oligopeptide transmembrane transporter activity
molecular_function GO:0015333 peptide:proton symporter activity
molecular_function GO:0005215 transporter activity
molecular_function GO:0022857 transmembrane transporter activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M001607Bhi03M001607mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 3..413
e-value: 5.2E-74
score: 249.3
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 1..452
NoneNo IPR availablePANTHERPTHR11654:SF181PROTEIN NRT1/ PTR FAMILY 2.8coord: 1..452
IPR036259MFS transporter superfamilySUPERFAMILYSSF103473MFS general substrate transportercoord: 31..134
coord: 207..446

The following gene(s) are paralogous to this gene:

None