Bhi03G001291 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi03G001291
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	Cysteine proteinase inhibitor
Location	chr3 : 35062111 .. 35062524 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAGTTTCTACAAAATATCAATCAAGGGTATTATATAATGAGTGAGATACCACCAAGTATTTGCCTTTGGTTTCCGATTAAGGATATCACTGATCCACATGTGCAAGAAATAGGAAAACTTCTGGTGGATGAGATTAACGAGGATAATACAAGGGAAAATCTGAAGTTCAAGCGTGTGGTGAATGGTCTGCGTTCAAACGAAGGCACATTATATAACCTTGTGTTGGAAGTTGAAACGTCCCAACAAATTAATTGGACATATGCGACCATCGTGTTAGAGGTTATCCAATCCGATGTACTAAAGCTTCGTATCATATCTTTTAAACCTGTTCTCGAGTACAAGATCTAATAATGAACTGTGCTCTCTACTTTACAAATTACAATATGCTTAAATAAGTTGTAACATTAACGTC

mRNA sequence

GAAAGTTTCTACAAAATATCAATCAAGGGTATTATATAATGAGTGAGATACCACCAAGTATTTGCCTTTGGTTTCCGATTAAGGATATCACTGATCCACATGTGCAAGAAATAGGAAAACTTCTGGTGGATGAGATTAACGAGGATAATACAAGGGAAAATCTGAAGTTCAAGCGTGTGGTGAATGGTCTGCGTTCAAACGAAGGCACATTATATAACCTTGTGTTGGAAGTTGAAACGTCCCAACAAATTAATTGGACATATGCGACCATCGTGTTAGAGGTTATCCAATCCGATGTACTAAAGCTTCGTATCATATCTTTTAAACCTGTTCTCGAGTACAAGATCTAATAATGAACTGTGCTCTCTACTTTACAAATTACAATATGCTTAAATAAGTTGTAACATTAACGTC

Coding sequence (CDS)

AAGTTTCTACAAAATATCAATCAAGGGTATTATATAATGAGTGAGATACCACCAAGTATTTGCCTTTGGTTTCCGATTAAGGATATCACTGATCCACATGTGCAAGAAATAGGAAAACTTCTGGTGGATGAGATTAACGAGGATAATACAAGGGAAAATCTGAAGTTCAAGCGTGTGGTGAATGGTCTGCGTTCAAACGAAGGCACATTATATAACCTTGTGTTGGAAGTTGAAACGTCCCAACAAATTAATTGGACATATGCGACCATCGTGTTAGAGGTTATCCAATCCGATGTACTAAAGCTTCGTATCATATCTTTTAAACCTGTTCTCGAGTACAAGATCTAA

Protein sequence

KFLQNINQGYYIMSEIPPSICLWFPIKDITDPHVQEIGKLLVDEINEDNTRENLKFKRVVNGLRSNEGTLYNLVLEVETSQQINWTYATIVLEVIQSDVLKLRIISFKPVLEYKI

Homology

[Close]

BLAST of Bhi03G001291 vs. Swiss-Prot
Match: sp|Q10Q46|CYT6_ORYSJ (Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210200 PE=3 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 8.2e-04
Identity = 31/87 (35.63%), Postives = 45/87 (51.72%), Query Frame = 0


Query: 23  WFPIKDITDPHVQEIGKLLVDEINEDNTRENLKFKRVVNGLRS-NEGTLYNLVLEVET-S 82
           W PIK+I DPH+QE+G+  + E N  +  + L F RV  G +    G  Y L +E  +  
Sbjct: 23  WSPIKNIDDPHIQELGRWAITENNRVSPSDELTFHRVTGGEQQVVSGMNYRLEIEAASGG 82

Query: 83  QQINWTYATIVLEVIQSDVLKLRIISF 108
             +  +Y  +V E   S+  KL  ISF
Sbjct: 83  GDVTGSYGAVVFEQEWSNTRKL--ISF 107

[Close]

BLAST of Bhi03G001291 vs. TrEMBL
Match: tr|A0A0A0L191|A0A0A0L191_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE=4 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.5e-13
Identity = 48/97 (49.48%), Postives = 65/97 (67.01%), Query Frame = 0


Query: 25  PIKDITDPHVQEIGKLLVDEINEDNTRENLKFKRVVNGLRSN--------EGTLYNLVLE 84
           PI DI DPHVQ IGKL V++ +  N  E LKF RVVNGL+S+        E  LY+LVLE
Sbjct: 12  PIIDINDPHVQIIGKLAVEDHDSHNPGEKLKFVRVVNGLKSDKYIGPGFTEVILYHLVLE 71

Query: 85  VETSQQINWTYATIVLEVIQSDVLKLRIISFKPVLEY 114
            +T+++INWTYAT + +V    +++   +SF+PVL Y
Sbjct: 72  AKTNEEINWTYATKLEKVYSGCLIRHVFLSFEPVLPY 108

[Close]

BLAST of Bhi03G001291 vs. TrEMBL
Match: tr|A0A0A0KZ98|A0A0A0KZ98_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508510 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 4.2e-13
Identity = 47/97 (48.45%), Postives = 65/97 (67.01%), Query Frame = 0


Query: 25  PIKDITDPHVQEIGKLLVDEINEDNTRENLKFKRVVNGLRSN--------EGTLYNLVLE 84
           PI DI DPHVQ +G+L V+ +   N+ E LKF RVVNGL+S+        E  LY+LVLE
Sbjct: 12  PIIDINDPHVQALGELAVENLYSKNSGEKLKFVRVVNGLKSDKYIGPGFTEVILYHLVLE 71

Query: 85  VETSQQINWTYATIVLEVIQSDVLKLRIISFKPVLEY 114
            +T+++INWTYAT  LE +   +++   +SF+PVL Y
Sbjct: 72  AKTNEEINWTYAT-KLEEVSGGLIRHVFLSFEPVLPY 107

[Close]

BLAST of Bhi03G001291 vs. TrEMBL
Match: tr|A0A1S3B5B9|A0A1S3B5B9_CUCME (Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103486185 PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.5e-07
Identity = 46/102 (45.10%), Postives = 59/102 (57.84%), Query Frame = 0


Query: 25  PIKDITDP-HVQEIGKLLVDEINEDNTRENLKFKRVVNGLRSN--------EGTLYNLVL 84
           PI+DI D   VQ++G L VD+ N+  T ENLKF RVVNGL ++         G LY LVL
Sbjct: 9   PIEDINDNLEVQDLGNLTVDDHNK-KTGENLKFVRVVNGLAASFSDPISPLGGVLYELVL 68

Query: 85  EVETSQQINWTYATIVLE---VIQSDVLKLRIISFKPVLEYK 115
           E  T+ +INWTY   V +   +     +     SFKPVLEY+
Sbjct: 69  EAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEYE 109

[Close]

BLAST of Bhi03G001291 vs. TrEMBL
Match: tr|A0A0A0L3W1|A0A0A0L3W1_CUCSA (Proteinase inhibitor I25, cystatin OS=Cucumis sativus OX=3659 GN=Csa_4G508490 PE=4 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 6.5e-06
Identity = 46/118 (38.98%), Postives = 68/118 (57.63%), Query Frame = 0


Query: 7   NQGYYIMSEIPPSICLWFPIKDITDP-HVQE-IGKLLVDEINEDNTRENLKFKRVVNGLR 66
           +Q Y IMS +  +     PI+DI D   VQ  IG+L V++ N+  T +NL+   VVNGLR
Sbjct: 4   HQRYCIMS-VEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNK-KTGDNLELVDVVNGLR 63

Query: 67  S---------NEGTLYNLVLEVETSQQINWTYATIVLEVIQSDVLKLRIISFKPVLEY 114
           S         +EG LY+L++E +T + INWTY   +LE+     ++    SF+P+L Y
Sbjct: 64  SGIFVQPGSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIRYEFKSFEPLLPY 119

[Close]

BLAST of Bhi03G001291 vs. TrEMBL
Match: tr|A0A0D9WYJ1|A0A0D9WYJ1_9ORYZ (Cysteine proteinase inhibitor OS=Leersia perrieri OX=77586 PE=3 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 4.7e-04
Identity = 28/72 (38.89%), Postives = 40/72 (55.56%), Query Frame = 0


Query: 23  WFPIKDITDPHVQEIGKLLVDEINEDNTRENLKFKRVVNGLRS-NEGTLYNLVLEVETSQ 82
           W PI+DI DPH+QE+G+  V E N+ +T   L F +V +G +    G  Y L +   TS 
Sbjct: 34  WSPIEDINDPHIQELGEWAVSEHNKVSTTSVLTFSKVTSGEQQVVSGMNYRLFIAASTSN 93

Query: 83  QINWTYATIVLE 94
             N +Y  +V E
Sbjct: 94  DTNGSYVAVVFE 105

[Close]

BLAST of Bhi03G001291 vs. NCBI nr
Match: KGN54824.1 (hypothetical protein Csa_4G508520 [Cucumis sativus])

HSP 1 Score: 84.0 bits (206), Expect = 3.7e-13
Identity = 48/97 (49.48%), Postives = 65/97 (67.01%), Query Frame = 0


Query: 25  PIKDITDPHVQEIGKLLVDEINEDNTRENLKFKRVVNGLRSN--------EGTLYNLVLE 84
           PI DI DPHVQ IGKL V++ +  N  E LKF RVVNGL+S+        E  LY+LVLE
Sbjct: 12  PIIDINDPHVQIIGKLAVEDHDSHNPGEKLKFVRVVNGLKSDKYIGPGFTEVILYHLVLE 71

Query: 85  VETSQQINWTYATIVLEVIQSDVLKLRIISFKPVLEY 114
            +T+++INWTYAT + +V    +++   +SF+PVL Y
Sbjct: 72  AKTNEEINWTYATKLEKVYSGCLIRHVFLSFEPVLPY 108

[Close]

BLAST of Bhi03G001291 vs. NCBI nr
Match: KGN54823.1 (hypothetical protein Csa_4G508510 [Cucumis sativus])

HSP 1 Score: 83.2 bits (204), Expect = 6.4e-13
Identity = 47/97 (48.45%), Postives = 65/97 (67.01%), Query Frame = 0


Query: 25  PIKDITDPHVQEIGKLLVDEINEDNTRENLKFKRVVNGLRSN--------EGTLYNLVLE 84
           PI DI DPHVQ +G+L V+ +   N+ E LKF RVVNGL+S+        E  LY+LVLE
Sbjct: 12  PIIDINDPHVQALGELAVENLYSKNSGEKLKFVRVVNGLKSDKYIGPGFTEVILYHLVLE 71

Query: 85  VETSQQINWTYATIVLEVIQSDVLKLRIISFKPVLEY 114
            +T+++INWTYAT  LE +   +++   +SF+PVL Y
Sbjct: 72  AKTNEEINWTYAT-KLEEVSGGLIRHVFLSFEPVLPY 107

[Close]

BLAST of Bhi03G001291 vs. NCBI nr
Match: XP_008442277.1 (PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo])

HSP 1 Score: 63.5 bits (153), Expect = 5.2e-07
Identity = 46/102 (45.10%), Postives = 59/102 (57.84%), Query Frame = 0


Query: 25  PIKDITDP-HVQEIGKLLVDEINEDNTRENLKFKRVVNGLRSN--------EGTLYNLVL 84
           PI+DI D   VQ++G L VD+ N+  T ENLKF RVVNGL ++         G LY LVL
Sbjct: 9   PIEDINDNLEVQDLGNLTVDDHNK-KTGENLKFVRVVNGLAASFSDPISPLGGVLYELVL 68

Query: 85  EVETSQQINWTYATIVLE---VIQSDVLKLRIISFKPVLEYK 115
           E  T+ +INWTY   V +   +     +     SFKPVLEY+
Sbjct: 69  EAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEYE 109

[Close]

BLAST of Bhi03G001291 vs. NCBI nr
Match: KGN54821.1 (Proteinase inhibitor I25, cystatin [Cucumis sativus])

HSP 1 Score: 59.3 bits (142), Expect = 9.9e-06
Identity = 46/118 (38.98%), Postives = 68/118 (57.63%), Query Frame = 0


Query: 7   NQGYYIMSEIPPSICLWFPIKDITDP-HVQE-IGKLLVDEINEDNTRENLKFKRVVNGLR 66
           +Q Y IMS +  +     PI+DI D   VQ  IG+L V++ N+  T +NL+   VVNGLR
Sbjct: 4   HQRYCIMS-VEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNK-KTGDNLELVDVVNGLR 63

Query: 67  S---------NEGTLYNLVLEVETSQQINWTYATIVLEVIQSDVLKLRIISFKPVLEY 114
           S         +EG LY+L++E +T + INWTY   +LE+     ++    SF+P+L Y
Sbjct: 64  SGIFVQPGSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIRYEFKSFEPLLPY 119

The following BLAST results are available for this feature:

BLAST of Bhi03G001291 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q10Q46\|CYT6_ORYSJ	8.2e-04	35.63	Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03...	[more]

BLAST of Bhi03G001291 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of Bhi03G001291 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0L191\|A0A0A0L191_CUCSA	2.5e-13	49.48	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE=4 SV=1	[more]
tr\|A0A0A0KZ98\|A0A0A0KZ98_CUCSA	4.2e-13	48.45	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508510 PE=4 SV=1	[more]
tr\|A0A1S3B5B9\|A0A1S3B5B9_CUCME	3.5e-07	45.10	Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103486185 PE=3 SV=1	[more]
tr\|A0A0A0L3W1\|A0A0A0L3W1_CUCSA	6.5e-06	38.98	Proteinase inhibitor I25, cystatin OS=Cucumis sativus OX=3659 GN=Csa_4G508490 PE...	[more]
tr\|A0A0D9WYJ1\|A0A0D9WYJ1_9ORYZ	4.7e-04	38.89	Cysteine proteinase inhibitor OS=Leersia perrieri OX=77586 PE=3 SV=1	[more]

BLAST of Bhi03G001291 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN54824.1	3.7e-13	49.48	hypothetical protein Csa_4G508520 [Cucumis sativus]	[more]
KGN54823.1	6.4e-13	48.45	hypothetical protein Csa_4G508510 [Cucumis sativus]	[more]
XP_008442277.1	5.2e-07	45.10	PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo]	[more]
KGN54821.1	9.9e-06	38.98	Proteinase inhibitor I25, cystatin [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004869	cysteine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR027214	Cystatin
IPR000010	Cystatin_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi03M001291	Bhi03M001291	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 23..114 e-value: 6.1E-13 score: 50.6
None	No IPR available	PANTHER	PTHR11413:SF65	CYSTEINE PROTEINASE INHIBITOR 5	coord: 22..109
None	No IPR available	SUPERFAMILY	SSF54403	Cystatin/monellin	coord: 23..110
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 26..109 e-value: 1.0E-12 score: 48.1
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 22..109

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi03G001291	Wax gourd	wgowgoB012
Bhi03G001291	Cucumber (Gy14) v1	cgywgoB068
Bhi03G001291	Cucumber (Gy14) v1	cgywgoB615
Bhi03G001291	Cucumber (Gy14) v2	cgybwgoB316
Bhi03G001291	Cucumber (Gy14) v2	cgybwgoB388
Bhi03G001291	Cucurbita maxima (Rimu)	cmawgoB0353
Bhi03G001291	Cucurbita maxima (Rimu)	cmawgoB0803
Bhi03G001291	Cucurbita maxima (Rimu)	cmawgoB1035
Bhi03G001291	Cucurbita maxima (Rimu)	cmawgoB1073
Bhi03G001291	Cucurbita moschata (Rifu)	cmowgoB0343
Bhi03G001291	Cucurbita moschata (Rifu)	cmowgoB0797
Bhi03G001291	Cucurbita moschata (Rifu)	cmowgoB1029
Bhi03G001291	Cucurbita moschata (Rifu)	cmowgoB1067
Bhi03G001291	Cucurbita pepo (Zucchini)	cpewgoB0091
Bhi03G001291	Cucurbita pepo (Zucchini)	cpewgoB0600
Bhi03G001291	Cucurbita pepo (Zucchini)	cpewgoB0810
Bhi03G001291	Cucurbita pepo (Zucchini)	cpewgoB1133
Bhi03G001291	Wild cucumber (PI 183967)	cpiwgoB328
Bhi03G001291	Wild cucumber (PI 183967)	cpiwgoB410
Bhi03G001291	Cucumber (Chinese Long) v3	cucwgoB332
Bhi03G001291	Cucumber (Chinese Long) v3	cucwgoB411
Bhi03G001291	Cucumber (Chinese Long) v2	cuwgoB325
Bhi03G001291	Cucumber (Chinese Long) v2	cuwgoB407
Bhi03G001291	Bottle gourd (USVL1VR-Ls)	lsiwgoB184
Bhi03G001291	Bottle gourd (USVL1VR-Ls)	lsiwgoB334
Bhi03G001291	Melon (DHL92) v3.6.1	medwgoB427
Bhi03G001291	Melon (DHL92) v3.6.1	medwgoB486
Bhi03G001291	Melon (DHL92) v3.5.1	mewgoB424
Bhi03G001291	Melon (DHL92) v3.5.1	mewgoB485
Bhi03G001291	Watermelon (Charleston Gray)	wcgwgoB126
Bhi03G001291	Watermelon (Charleston Gray)	wcgwgoB383
Bhi03G001291	Watermelon (97103) v2	wgowmbB412
Bhi03G001291	Watermelon (97103) v2	wgowmbB423
Bhi03G001291	Watermelon (97103) v1	wgowmB412
Bhi03G001291	Watermelon (97103) v1	wgowmB438
Bhi03G001291	Silver-seed gourd	carwgoB0210
Bhi03G001291	Silver-seed gourd	carwgoB0504
Bhi03G001291	Silver-seed gourd	carwgoB0658