Bhi03G001224 (gene) Wax gourd

NameBhi03G001224
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionPhenylalanine ammonia-lyase
Locationchr3 : 33473541 .. 33475682 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACCTACTCACTTCCAACAACAGAATGGTTCGATGGAGAGCTTGTGCATTAGCCAAAACGATCCACTCAATTGGGGTGTGGCCGCAGATTCCCTCAAGGGAAGCCATCTCGATGAGGTGAAGCACATGATCGAGGAGTATCGGAGGCCTATTGTCAAGATTGGTGGTGAAAGTCTCACCATTTCTCAAGTGGCTGCCATTGCCACTCGAGACTCTGATGTTATTGTTGAACTTTCTGAGTCTGCTAGAGCTGGTGTTAAGGCTAGTAGTGATTGGGTTATGGAAAGCATGAATAAAGGGACGGATAGTTATGGTGTTACTACTGGGTTTGGTGCTACATCTCATAGAAGGACCAAACAAGGTGGTGCTCTCCAAAAGGAGCTTATTAGATTTTTGAATGCTGGGATTTTTGGAAATGGAGCTGAGTCTGATCATACTTTGCCCCACTCGGCTACAAGGGCGGCCATGTTGGTGAGAATCAATACTCTTCTTCAAGGCTACTCGGGTATTAGATTTGAGATTTTGGAAGCTATAACCAAACTTTTGAACCACAACATTACTCCATGCTTGCCTCTTCGTGGAACAATTACTGCCTCTGGAGATCTTGTTCCTCTTTCTTATATAGCTGGACTGCTCACTGGGCGACCCAACTCCAAGGCGATTGGACCCAATGGAGAACGTATGGACGCTAAGGAGGCTTTCCAACAAGCTGGTATTCCTTCTGGGTTTTTCGAATTGCAGCCTAAGGAAGGTCTTGCTCTAGTTAATGGTACTGCTGTTGGTTCTGGATTGGCTTCTATTGTTCTCTTTGAGGCAAATATTTTGGCTGTTTTGTCAGAAATTCTATCTGCTATTTTTGCTGAGGTCATGCAAGGAAAGCCAGAATTTACTGATCATTTGACTCATAAGTTGAAGCACCACCCAGGTCAAATTGAAGCAGCAGCAATTATGGAGCACATTTTGGATGGAAGCTCTTACATGAAAACAGCCAAGAAATTACATGAAATCGATCCCCTCCAAAAACCCAAACAAGATCGTTATGCTCTAAGAACTTCACCTCAATGGCTTGGTCCGTTAATTGAAGTTATTCGATTCTCTACAAAATCTATTGAGCGAGAGATTAACTCGGTCAATGACAACCCTTTGATTGATGTTTCAAGAAACAAAGCATTGCATGGAGGCAACTTTCAGGGAACACCGATTGGAGTTTCCATGGACAACACTCGGTTGGCCATTGCTTCTATTGGGAAGCTCATGTTTGCCCAATTCTCTGAACTTGTTAATGATTTCTACAACAACGGTTTGCCTTCTAATCTTTCTGCAAGTAGAAATCCGAGTTTGGATTATGGGTTTAAAGGAGCTGAAATTGCCATGGCTTCATACTGCTCCGAGCTTCAATACCTTGCAAATCCAGTGACAAGCCATGTTCAGAGTGCCGAGCAACACAACCAAGATGTTAACTCTCTAGGTTTAATTTCATCAAGGAAAACAGCTGAAGCCATTGATATTCTTAAACTCATGTCATCTACATTTTTGGTTGCTCTTTGCCAAGCAATTGACTTAAGGCATTTGGAGGAAAACTTGAAGAGTACAGTGAAAAGCACTGTTAGTCAAGTAGCCAAGAAAGTTTTAACCACGTGCTCTAATGGTGCACTTCACCCATCAAGATTTTGTGAAAAAGATCTACTCAAAGTTGTTGATAGAGAGTACACATTTGCTTATATCGACGATCCATGCAATGCTACTTACCCATTGAGTCAAAAGTTGAGACAAGTTCTCGTTGAGCATGCACTTGCAAACGGTGATGATGAAAAGAATGCAGACACTTCAATCTTTCAAAAGATTGTTTTATTCGAGGAGGAACTAAAAGCATTATTACCCAAAGAAGTTGAAAATGCAAGGTTGGCCTATGAGACTGGGAACTCCAAGATTGAAAATCAAATCAAGGATTGTAGATCGTATCCAATATATAGATTTGTGAGAGAAGAGTTGGAAACAAAAATGCTAACTGGAGAAAAAGTGATATCTCCAGGAGAAGAGTGCGAGAAAGTATTCATAGCTTTGTGTCAAGGCAAGATGATAGACTCGATCTTAGAGTGTTTGAAGGAGTGGAACGGTGCTCCAATTCCAATTTGCTAA

mRNA sequence

ATGGCACCTACTCACTTCCAACAACAGAATGGTTCGATGGAGAGCTTGTGCATTAGCCAAAACGATCCACTCAATTGGGGTGTGGCCGCAGATTCCCTCAAGGGAAGCCATCTCGATGAGGTGAAGCACATGATCGAGGAGTATCGGAGGCCTATTGTCAAGATTGGTGGTGAAAGTCTCACCATTTCTCAAGTGGCTGCCATTGCCACTCGAGACTCTGATGTTATTGTTGAACTTTCTGAGTCTGCTAGAGCTGGTGTTAAGGCTAGTAGTGATTGGGTTATGGAAAGCATGAATAAAGGGACGGATAGTTATGGTGTTACTACTGGGTTTGGTGCTACATCTCATAGAAGGACCAAACAAGGTGGTGCTCTCCAAAAGGAGCTTATTAGATTTTTGAATGCTGGGATTTTTGGAAATGGAGCTGAGTCTGATCATACTTTGCCCCACTCGGCTACAAGGGCGGCCATGTTGGTGAGAATCAATACTCTTCTTCAAGGCTACTCGGGTATTAGATTTGAGATTTTGGAAGCTATAACCAAACTTTTGAACCACAACATTACTCCATGCTTGCCTCTTCGTGGAACAATTACTGCCTCTGGAGATCTTGTTCCTCTTTCTTATATAGCTGGACTGCTCACTGGGCGACCCAACTCCAAGGCGATTGGACCCAATGGAGAACGTATGGACGCTAAGGAGGCTTTCCAACAAGCTGGTATTCCTTCTGGGTTTTTCGAATTGCAGCCTAAGGAAGGTCTTGCTCTAGTTAATGGTACTGCTGTTGGTTCTGGATTGGCTTCTATTGTTCTCTTTGAGGCAAATATTTTGGCTGTTTTGTCAGAAATTCTATCTGCTATTTTTGCTGAGGTCATGCAAGGAAAGCCAGAATTTACTGATCATTTGACTCATAAGTTGAAGCACCACCCAGGTCAAATTGAAGCAGCAGCAATTATGGAGCACATTTTGGATGGAAGCTCTTACATGAAAACAGCCAAGAAATTACATGAAATCGATCCCCTCCAAAAACCCAAACAAGATCGTTATGCTCTAAGAACTTCACCTCAATGGCTTGGTCCGTTAATTGAAGTTATTCGATTCTCTACAAAATCTATTGAGCGAGAGATTAACTCGGTCAATGACAACCCTTTGATTGATGTTTCAAGAAACAAAGCATTGCATGGAGGCAACTTTCAGGGAACACCGATTGGAGTTTCCATGGACAACACTCGGTTGGCCATTGCTTCTATTGGGAAGCTCATGTTTGCCCAATTCTCTGAACTTGTTAATGATTTCTACAACAACGGTTTGCCTTCTAATCTTTCTGCAAGTAGAAATCCGAGTTTGGATTATGGGTTTAAAGGAGCTGAAATTGCCATGGCTTCATACTGCTCCGAGCTTCAATACCTTGCAAATCCAGTGACAAGCCATGTTCAGAGTGCCGAGCAACACAACCAAGATGTTAACTCTCTAGGTTTAATTTCATCAAGGAAAACAGCTGAAGCCATTGATATTCTTAAACTCATGTCATCTACATTTTTGGTTGCTCTTTGCCAAGCAATTGACTTAAGGCATTTGGAGGAAAACTTGAAGAGTACAGTGAAAAGCACTGTTAGTCAAGTAGCCAAGAAAGTTTTAACCACGTGCTCTAATGGTGCACTTCACCCATCAAGATTTTGTGAAAAAGATCTACTCAAAGTTGTTGATAGAGAGTACACATTTGCTTATATCGACGATCCATGCAATGCTACTTACCCATTGAGTCAAAAGTTGAGACAAGTTCTCGTTGAGCATGCACTTGCAAACGGTGATGATGAAAAGAATGCAGACACTTCAATCTTTCAAAAGATTGTTTTATTCGAGGAGGAACTAAAAGCATTATTACCCAAAGAAGTTGAAAATGCAAGGTTGGCCTATGAGACTGGGAACTCCAAGATTGAAAATCAAATCAAGGATTGTAGATCGTATCCAATATATAGATTTGTGAGAGAAGAGTTGGAAACAAAAATGCTAACTGGAGAAAAAGTGATATCTCCAGGAGAAGAGTGCGAGAAAGTATTCATAGCTTTGTGTCAAGGCAAGATGATAGACTCGATCTTAGAGTGTTTGAAGGAGTGGAACGGTGCTCCAATTCCAATTTGCTAA

Coding sequence (CDS)

ATGGCACCTACTCACTTCCAACAACAGAATGGTTCGATGGAGAGCTTGTGCATTAGCCAAAACGATCCACTCAATTGGGGTGTGGCCGCAGATTCCCTCAAGGGAAGCCATCTCGATGAGGTGAAGCACATGATCGAGGAGTATCGGAGGCCTATTGTCAAGATTGGTGGTGAAAGTCTCACCATTTCTCAAGTGGCTGCCATTGCCACTCGAGACTCTGATGTTATTGTTGAACTTTCTGAGTCTGCTAGAGCTGGTGTTAAGGCTAGTAGTGATTGGGTTATGGAAAGCATGAATAAAGGGACGGATAGTTATGGTGTTACTACTGGGTTTGGTGCTACATCTCATAGAAGGACCAAACAAGGTGGTGCTCTCCAAAAGGAGCTTATTAGATTTTTGAATGCTGGGATTTTTGGAAATGGAGCTGAGTCTGATCATACTTTGCCCCACTCGGCTACAAGGGCGGCCATGTTGGTGAGAATCAATACTCTTCTTCAAGGCTACTCGGGTATTAGATTTGAGATTTTGGAAGCTATAACCAAACTTTTGAACCACAACATTACTCCATGCTTGCCTCTTCGTGGAACAATTACTGCCTCTGGAGATCTTGTTCCTCTTTCTTATATAGCTGGACTGCTCACTGGGCGACCCAACTCCAAGGCGATTGGACCCAATGGAGAACGTATGGACGCTAAGGAGGCTTTCCAACAAGCTGGTATTCCTTCTGGGTTTTTCGAATTGCAGCCTAAGGAAGGTCTTGCTCTAGTTAATGGTACTGCTGTTGGTTCTGGATTGGCTTCTATTGTTCTCTTTGAGGCAAATATTTTGGCTGTTTTGTCAGAAATTCTATCTGCTATTTTTGCTGAGGTCATGCAAGGAAAGCCAGAATTTACTGATCATTTGACTCATAAGTTGAAGCACCACCCAGGTCAAATTGAAGCAGCAGCAATTATGGAGCACATTTTGGATGGAAGCTCTTACATGAAAACAGCCAAGAAATTACATGAAATCGATCCCCTCCAAAAACCCAAACAAGATCGTTATGCTCTAAGAACTTCACCTCAATGGCTTGGTCCGTTAATTGAAGTTATTCGATTCTCTACAAAATCTATTGAGCGAGAGATTAACTCGGTCAATGACAACCCTTTGATTGATGTTTCAAGAAACAAAGCATTGCATGGAGGCAACTTTCAGGGAACACCGATTGGAGTTTCCATGGACAACACTCGGTTGGCCATTGCTTCTATTGGGAAGCTCATGTTTGCCCAATTCTCTGAACTTGTTAATGATTTCTACAACAACGGTTTGCCTTCTAATCTTTCTGCAAGTAGAAATCCGAGTTTGGATTATGGGTTTAAAGGAGCTGAAATTGCCATGGCTTCATACTGCTCCGAGCTTCAATACCTTGCAAATCCAGTGACAAGCCATGTTCAGAGTGCCGAGCAACACAACCAAGATGTTAACTCTCTAGGTTTAATTTCATCAAGGAAAACAGCTGAAGCCATTGATATTCTTAAACTCATGTCATCTACATTTTTGGTTGCTCTTTGCCAAGCAATTGACTTAAGGCATTTGGAGGAAAACTTGAAGAGTACAGTGAAAAGCACTGTTAGTCAAGTAGCCAAGAAAGTTTTAACCACGTGCTCTAATGGTGCACTTCACCCATCAAGATTTTGTGAAAAAGATCTACTCAAAGTTGTTGATAGAGAGTACACATTTGCTTATATCGACGATCCATGCAATGCTACTTACCCATTGAGTCAAAAGTTGAGACAAGTTCTCGTTGAGCATGCACTTGCAAACGGTGATGATGAAAAGAATGCAGACACTTCAATCTTTCAAAAGATTGTTTTATTCGAGGAGGAACTAAAAGCATTATTACCCAAAGAAGTTGAAAATGCAAGGTTGGCCTATGAGACTGGGAACTCCAAGATTGAAAATCAAATCAAGGATTGTAGATCGTATCCAATATATAGATTTGTGAGAGAAGAGTTGGAAACAAAAATGCTAACTGGAGAAAAAGTGATATCTCCAGGAGAAGAGTGCGAGAAAGTATTCATAGCTTTGTGTCAAGGCAAGATGATAGACTCGATCTTAGAGTGTTTGAAGGAGTGGAACGGTGCTCCAATTCCAATTTGCTAA

Protein sequence

MAPTHFQQQNGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC
BLAST of Bhi03G001224 vs. Swiss-Prot
Match: sp|P45730|PALY_POPTR (Phenylalanine ammonia-lyase OS=Populus trichocarpa OX=3694 GN=PAL PE=2 SV=1)

HSP 1 Score: 1217.6 bits (3149), Expect = 0.0e+00
Identity = 605/706 (85.69%), Postives = 664/706 (94.05%), Query Frame = 0

Query: 9   QNGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAI 68
           QNGS+ESLC++Q DPL+WGVAA+++KGSHLDEVK M+ +YR+P+VK+GGE+LTI+QVA+I
Sbjct: 10  QNGSLESLCVNQRDPLSWGVAAEAMKGSHLDEVKRMVADYRKPVVKLGGETLTIAQVASI 69

Query: 69  ATRDS-DVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQK 128
           A  D+ DV VELSESAR GVKASSDWVM+SM+KGTDSYGVTTGFGATSHRRTKQGGALQK
Sbjct: 70  AGHDTGDVKVELSESARPGVKASSDWVMDSMDKGTDSYGVTTGFGATSHRRTKQGGALQK 129

Query: 129 ELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNI 188
           ELIRFLNAGIFGNG E+ HTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIT+LLN+NI
Sbjct: 130 ELIRFLNAGIFGNGTETCHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITRLLNNNI 189

Query: 189 TPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFEL 248
           TPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA GP GE +DA EAF+ AGI SGFFEL
Sbjct: 190 TPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKATGPTGEVLDAAEAFKAAGIESGFFEL 249

Query: 249 QPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKH 308
           QPKEGLALVNGTAVGSGLAS+VLFE N+LAVLSE+LSAIFAEVM GKPEFTDHLTHKLKH
Sbjct: 250 QPKEGLALVNGTAVGSGLASMVLFETNVLAVLSELLSAIFAEVMNGKPEFTDHLTHKLKH 309

Query: 309 HPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFS 368
           HPGQIEAAAIMEHILDGS+YMK AKKLHE DPLQKPKQDRYALRTSPQWLGP IEVIRFS
Sbjct: 310 HPGQIEAAAIMEHILDGSAYMKAAKKLHETDPLQKPKQDRYALRTSPQWLGPQIEVIRFS 369

Query: 369 TKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSEL 428
           TKSIEREINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDN RLAIASIGKL+FAQFSEL
Sbjct: 370 TKSIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNVRLAIASIGKLLFAQFSEL 429

Query: 429 VNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDV 488
           VNDFYNNGLPSNL+ASRNPSLDYGFKGAEIAMASYCSELQYLANPVT+HVQSAEQHNQDV
Sbjct: 430 VNDFYNNGLPSNLTASRNPSLDYGFKGAEIAMASYCSELQYLANPVTTHVQSAEQHNQDV 489

Query: 489 NSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTT 548
           NSLGLISSRKTAEA+DILKLMS+TFLVALCQAIDLRHLEENLKS VK+TVSQV+K+VLTT
Sbjct: 490 NSLGLISSRKTAEAVDILKLMSTTFLVALCQAIDLRHLEENLKSAVKNTVSQVSKRVLTT 549

Query: 549 CSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKN 608
            +NG LHPSRFCEK+LLKVVDREY FAY+DDPC+ATYPL QKLRQV V+HAL NG++EKN
Sbjct: 550 GANGELHPSRFCEKELLKVVDREYVFAYVDDPCSATYPLMQKLRQVFVDHALENGENEKN 609

Query: 609 ADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELET 668
             TS+FQKI  FEEELKALLPKEVE+AR AY++GNS I+N+IK+CRSYP+Y+FVREEL T
Sbjct: 610 FSTSVFQKIEAFEEELKALLPKEVESARAAYDSGNSAIDNKIKECRSYPLYKFVREELGT 669

Query: 669 KMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
            +LTGEKV SPGEE +KVF A+CQGK+ID +LECL EWNG+P+PIC
Sbjct: 670 VLLTGEKVQSPGEEFDKVFTAMCQGKIIDPMLECLGEWNGSPLPIC 715

BLAST of Bhi03G001224 vs. Swiss-Prot
Match: sp|P45732|PALY_STYHU (Phenylalanine ammonia-lyase OS=Stylosanthes humilis OX=35628 GN=PAL17.1 PE=2 SV=1)

HSP 1 Score: 1209.1 bits (3127), Expect = 0.0e+00
Identity = 598/694 (86.17%), Postives = 651/694 (93.80%), Query Frame = 0

Query: 20  QNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIATRDSDVIVEL 79
           ++DPLNW  AA++LKGSHLDEVK M+ EYR+P+V +GG++LTISQVAAIA  D  V V+L
Sbjct: 22  RHDPLNWAAAAEALKGSHLDEVKRMVSEYRKPLVNLGGQTLTISQVAAIAANDQGVSVQL 81

Query: 80  SESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFG 139
           SE++RAGVKASSDWVM+SMN GTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFG
Sbjct: 82  SEASRAGVKASSDWVMDSMNNGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFG 141

Query: 140 NGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPLRGTITA 199
           NG E++ TLPH+ATRAAMLVRINTLLQGYSGIRFEILEAITKLLN+NITPCLPLRGTITA
Sbjct: 142 NGTETNCTLPHTATRAAMLVRINTLLQGYSGIRFEILEAITKLLNNNITPCLPLRGTITA 201

Query: 200 SGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQPKEGLALVNGT 259
           SGDLVPLSYIAGLLTGRPNSKA+GPNGE ++AKEAFQ AGI S FFELQPKEGLALVNGT
Sbjct: 202 SGDLVPLSYIAGLLTGRPNSKAVGPNGETLNAKEAFQAAGIGSDFFELQPKEGLALVNGT 261

Query: 260 AVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIME 319
            VGSGLAS+VLFEANILAVLSE+LSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIME
Sbjct: 262 PVGSGLASVVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIME 321

Query: 320 HILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVN 379
           HILDGSSY+K AKKLHEIDPLQKPKQDRYALRTSPQWLGPL+EVIRFSTKSIEREINSVN
Sbjct: 322 HILDGSSYVKAAKKLHEIDPLQKPKQDRYALRTSPQWLGPLVEVIRFSTKSIEREINSVN 381

Query: 380 DNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGLPSN 439
           DNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLA+ASIGKLMFAQFSELVNDFYNNGLPSN
Sbjct: 382 DNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAVASIGKLMFAQFSELVNDFYNNGLPSN 441

Query: 440 LSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTA 499
           LSASRNPSLDYGFKG EIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLIS+RKT 
Sbjct: 442 LSASRNPSLDYGFKGTEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISARKTN 501

Query: 500 EAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCSNGALHPSRFC 559
           EA++ILKLMS T+L+ALCQAIDLRHLEENLK+TVK+TVSQVAK+ LTT  NG LHPSRFC
Sbjct: 502 EAVEILKLMSPTYLIALCQAIDLRHLEENLKNTVKNTVSQVAKRTLTTGVNGELHPSRFC 561

Query: 560 EKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNADTSIFQKIVLF 619
           EKDLLK+VDREY FAYIDDPC+ATYPL QKLRQVLVEHALAN ++EKN +TSIFQKI  F
Sbjct: 562 EKDLLKIVDREYCFAYIDDPCSATYPLMQKLRQVLVEHALANAENEKNVNTSIFQKITTF 621

Query: 620 EEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKMLTGEKVISPG 679
           EEELK LLPKEVE AR+AYE G S I N+IK+CRSYP+Y+FVREEL T+MLTGEKV SPG
Sbjct: 622 EEELKTLLPKEVEGARIAYENGQSAIPNKIKECRSYPLYKFVREELGTEMLTGEKVRSPG 681

Query: 680 EECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           EEC+K+F A+CQGK+ID +LEC+ EWNGAP+P+C
Sbjct: 682 EECDKLFTAMCQGKIIDPLLECIGEWNGAPLPLC 715

BLAST of Bhi03G001224 vs. Swiss-Prot
Match: sp|P45734|PALY_TRISU (Phenylalanine ammonia-lyase OS=Trifolium subterraneum OX=3900 GN=PAL1 PE=3 SV=1)

HSP 1 Score: 1204.5 bits (3115), Expect = 0.0e+00
Identity = 601/704 (85.37%), Postives = 659/704 (93.61%), Query Frame = 0

Query: 10  NGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIA 69
           + +  ++ ++  DPLNWGVAA+++KGSHLDEVK M+EEYR+P+V++GGE+LTISQVAAIA
Sbjct: 23  HANANNMKVNAADPLNWGVAAEAMKGSHLDEVKRMVEEYRKPVVRLGGETLTISQVAAIA 82

Query: 70  TRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKEL 129
             D    VELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKEL
Sbjct: 83  AHDG-ATVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKEL 142

Query: 130 IRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITP 189
           IRFLNAGIFGNG ES+HTLPH+ATRAAMLVRINTLLQGYSGIRFEILEAITKLLN+NITP
Sbjct: 143 IRFLNAGIFGNGTESNHTLPHTATRAAMLVRINTLLQGYSGIRFEILEAITKLLNNNITP 202

Query: 190 CLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQP 249
           CLPLRGTITASGDLVPLSYIAGLLTGR NSKA GP+GE ++AKEAFQ AGI + FFELQP
Sbjct: 203 CLPLRGTITASGDLVPLSYIAGLLTGRSNSKAHGPSGEMLNAKEAFQLAGINAEFFELQP 262

Query: 250 KEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHP 309
           KEGLALVNGTAVGSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPEFTDHLTHKLKHHP
Sbjct: 263 KEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHP 322

Query: 310 GQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTK 369
           GQIEAAAIMEHIL GS+Y+K AKKLHE+DPLQKPKQDRYALRTSPQWLGPLIEVIRFSTK
Sbjct: 323 GQIEAAAIMEHILHGSAYVKDAKKLHEMDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTK 382

Query: 370 SIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVN 429
           SIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLA+ASIGKL+FAQFSELVN
Sbjct: 383 SIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLALASIGKLLFAQFSELVN 442

Query: 430 DFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNS 489
           DFYNNGLPSNLSASRNPSLDYGFKG+EIAMASYCSELQYLANPVT+HVQSAEQHNQDVNS
Sbjct: 443 DFYNNGLPSNLSASRNPSLDYGFKGSEIAMASYCSELQYLANPVTTHVQSAEQHNQDVNS 502

Query: 490 LGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCS 549
           LGLISSRKT EAI+IL+LMSSTFL+ALCQAIDLRHLEENLK++VK+TVSQVAKK LT   
Sbjct: 503 LGLISSRKTKEAIEILQLMSSTFLIALCQAIDLRHLEENLKNSVKNTVSQVAKKTLTIGV 562

Query: 550 NGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNAD 609
           +G LHPSRFCEKDLLKVVDRE+ F+YIDDPC+ATYPL+QKLRQVLV+HAL NG+ EKN++
Sbjct: 563 SGELHPSRFCEKDLLKVVDREHVFSYIDDPCSATYPLAQKLRQVLVDHALVNGESEKNSN 622

Query: 610 TSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKM 669
           TSIFQKI  FEEELK LLPKEVE+AR AYE GNS I N+I  CRSYP+Y+FVREEL T +
Sbjct: 623 TSIFQKIATFEEELKTLLPKEVESARTAYENGNSTIANKINGCRSYPLYKFVREELGTSL 682

Query: 670 LTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           LTGE+VISPGEEC+K+F A+CQGK+ID +L+CL EWNGAP+PIC
Sbjct: 683 LTGERVISPGEECDKLFTAMCQGKIIDPLLKCLGEWNGAPLPIC 725

BLAST of Bhi03G001224 vs. Swiss-Prot
Match: sp|Q04593|PAL2_PEA (Phenylalanine ammonia-lyase 2 OS=Pisum sativum OX=3888 GN=PAL2 PE=2 SV=1)

HSP 1 Score: 1198.3 bits (3099), Expect = 0.0e+00
Identity = 597/703 (84.92%), Postives = 657/703 (93.46%), Query Frame = 0

Query: 10  NGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIA 69
           N    ++ +S +DPLNWGVAA+++KGSHLDEVK M++EYR+P+V++GGE+LTISQVAAIA
Sbjct: 22  NAKNNNIKVSDSDPLNWGVAAEAMKGSHLDEVKRMVDEYRKPVVRLGGETLTISQVAAIA 81

Query: 70  TRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKEL 129
             D  V VELSESARAGVKASSDWVMESMNKGTDSYGVTT  GATSHRRTKQGGALQKEL
Sbjct: 82  AHDHGVKVELSESARAGVKASSDWVMESMNKGTDSYGVTTVHGATSHRRTKQGGALQKEL 141

Query: 130 IRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITP 189
           IRFLNAGIFGNG+ES HTLPH+ATRAAMLVRINTLLQGYSGIRFEILEAITKL+N+N+TP
Sbjct: 142 IRFLNAGIFGNGSESTHTLPHTATRAAMLVRINTLLQGYSGIRFEILEAITKLINNNVTP 201

Query: 190 CLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQP 249
           CL LRGTITASGDLVPLSYIAGLLTGRPNSKA GP+GE ++A+EAFQ AGI  GFFELQP
Sbjct: 202 CL-LRGTITASGDLVPLSYIAGLLTGRPNSKAHGPSGEILNAREAFQSAGINDGFFELQP 261

Query: 250 KEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHP 309
           KEGLALVNGTAVGSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPEFTDHLTHKLKHHP
Sbjct: 262 KEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHP 321

Query: 310 GQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTK 369
           GQIEAAAIMEHILDGS+Y+K AKKLHE+DPLQKPKQDRYALRTSPQWLGPLIEVIRFSTK
Sbjct: 322 GQIEAAAIMEHILDGSAYVKAAKKLHEMDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTK 381

Query: 370 SIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVN 429
           SIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLA+ASIGKL+FAQFSELVN
Sbjct: 382 SIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLALASIGKLLFAQFSELVN 441

Query: 430 DFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNS 489
           DFYNNGLPSNLSASRNPSLDYGFKG+EIAMASYCSELQYLANPVT+HVQSAEQHNQDVNS
Sbjct: 442 DFYNNGLPSNLSASRNPSLDYGFKGSEIAMASYCSELQYLANPVTTHVQSAEQHNQDVNS 501

Query: 490 LGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCS 549
           LGLISSRKT EAI+IL+LMSSTFL+ALCQAIDLRHLEENLK++VK+ VS VAK+ LTT  
Sbjct: 502 LGLISSRKTYEAIEILQLMSSTFLIALCQAIDLRHLEENLKNSVKNMVSHVAKRTLTTGI 561

Query: 550 NGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNAD 609
           NG LHPSRFCEKDLL+VVDRE+ F+YIDDPC+ATYPL QKLRQVLV+HAL NG+ EKN +
Sbjct: 562 NGELHPSRFCEKDLLRVVDREHVFSYIDDPCSATYPLMQKLRQVLVDHALVNGESEKNLN 621

Query: 610 TSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKM 669
           TSIFQKI  FE+ELK LLPKEVE+AR AYE GN+ I N+IK+CRSYP+Y+FVREEL T +
Sbjct: 622 TSIFQKIATFEDELKTLLPKEVESARGAYENGNTTISNKIKECRSYPLYKFVREELGTSL 681

Query: 670 LTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPI 713
           LTGEKVISPGEEC+K+F A+CQGK+ID +LECL +WNGAP+PI
Sbjct: 682 LTGEKVISPGEECDKLFTAICQGKIIDPLLECLGDWNGAPLPI 723

BLAST of Bhi03G001224 vs. Swiss-Prot
Match: sp|O64963|PAL1_PRUAV (Phenylalanine ammonia-lyase 1 OS=Prunus avium OX=42229 GN=PAL1 PE=2 SV=1)

HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 600/707 (84.87%), Postives = 655/707 (92.64%), Query Frame = 0

Query: 9   QNGSME--SLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVA 68
           +NGS+E   LCI + DPLNWGVAA++LKGSHLDEVK M+ EYR+P+VK+GGESLTISQVA
Sbjct: 12  KNGSVELPELCI-KKDPLNWGVAAETLKGSHLDEVKRMVAEYRKPVVKLGGESLTISQVA 71

Query: 69  AIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQ 128
           AIAT DS V VELSESARAGVKASSDWVM+SM+KGTDSYGVTTGFGATSHRRTKQG ALQ
Sbjct: 72  AIATHDSGVKVELSESARAGVKASSDWVMDSMSKGTDSYGVTTGFGATSHRRTKQGAALQ 131

Query: 129 KELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHN 188
           KELIRFLNAG+FG+  ES HTLPH ATRAAMLVRINTLLQGYSGIRFEILE ITK LN+N
Sbjct: 132 KELIRFLNAGVFGSTKESGHTLPHQATRAAMLVRINTLLQGYSGIRFEILEVITKFLNNN 191

Query: 189 ITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFE 248
           +TPCLPLRGTITASGDLVPLSYIAG+LTGRPNSKA+GP+G+ + A EAF+  GI SGFFE
Sbjct: 192 VTPCLPLRGTITASGDLVPLSYIAGMLTGRPNSKAVGPDGQTLSAAEAFEFVGINSGFFE 251

Query: 249 LQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLK 308
           LQPKEGLALVNGTAVGSGLAS VLF+ NILA+LSEILSAIFAEVMQGKPEFTDHLTHKLK
Sbjct: 252 LQPKEGLALVNGTAVGSGLASTVLFDTNILALLSEILSAIFAEVMQGKPEFTDHLTHKLK 311

Query: 309 HHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRF 368
           HHPGQIEAAAIMEHILDGSSY+K AKKLHE DPLQKPKQDRYALRTSPQWLGP IEVIR+
Sbjct: 312 HHPGQIEAAAIMEHILDGSSYVKAAKKLHEQDPLQKPKQDRYALRTSPQWLGPQIEVIRY 371

Query: 369 STKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSE 428
           STKSIEREI+SVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSE
Sbjct: 372 STKSIEREIDSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSE 431

Query: 429 LVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQD 488
           LVNDFYNNGLPSNLS  RNPSLDYGFKGAEIAMASYCSELQ+LANPVT+HVQSAEQHNQD
Sbjct: 432 LVNDFYNNGLPSNLSGGRNPSLDYGFKGAEIAMASYCSELQFLANPVTNHVQSAEQHNQD 491

Query: 489 VNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLT 548
           VNSLGLISSRKTAEA+DILKLMSSTFLVALCQAIDLRHLEENL++TVK+TVSQVAK+ LT
Sbjct: 492 VNSLGLISSRKTAEAVDILKLMSSTFLVALCQAIDLRHLEENLRNTVKNTVSQVAKRTLT 551

Query: 549 TCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEK 608
           T  NG LHPSRFCEKDLLKVVDREY FAYIDDPC+ATYPL QKLRQVLVEHAL NG++EK
Sbjct: 552 TGVNGELHPSRFCEKDLLKVVDREYVFAYIDDPCSATYPLMQKLRQVLVEHALTNGENEK 611

Query: 609 NADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELE 668
           NA TSIFQKIV FEEELK LLPKEV++AR A ++G++ + N+I +CRSYP+Y+FVREEL 
Sbjct: 612 NASTSIFQKIVAFEEELKVLLPKEVDSARAALDSGSAGVPNRITECRSYPLYKFVREELG 671

Query: 669 TKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
            + LTGEKV SPGEEC+KVF A+C+GK+ID IL+CL+ WNGAP+PIC
Sbjct: 672 AEYLTGEKVRSPGEECDKVFTAICEGKIIDPILDCLEGWNGAPLPIC 717

BLAST of Bhi03G001224 vs. TAIR10
Match: AT3G53260.1 (phenylalanine ammonia-lyase 2)

HSP 1 Score: 1170.6 bits (3027), Expect = 0.0e+00
Identity = 580/692 (83.82%), Postives = 640/692 (92.49%), Query Frame = 0

Query: 22  DPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIATRDSDVIVELSE 81
           DPLNWG+AAD +KGSHLDEVK M+EEYRRP+V +GGE+LTI QVAAI+T    V VEL+E
Sbjct: 26  DPLNWGLAADQMKGSHLDEVKKMVEEYRRPVVNLGGETLTIGQVAAISTVGGSVKVELAE 85

Query: 82  SARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGNG 141
           ++RAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK G ALQ ELIRFLNAGIFGN 
Sbjct: 86  TSRAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGTALQTELIRFLNAGIFGNT 145

Query: 142 AESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPLRGTITASG 201
            E+ HTLP SATRAAMLVR+NTLLQGYSGIRFEILEAIT LLNHNI+P LPLRGTITASG
Sbjct: 146 KETCHTLPQSATRAAMLVRVNTLLQGYSGIRFEILEAITSLLNHNISPSLPLRGTITASG 205

Query: 202 DLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQPKEGLALVNGTAV 261
           DLVPLSYIAGLLTGRPNSKA GP+GE + AKEAF++AGI +GFF+LQPKEGLALVNGTAV
Sbjct: 206 DLVPLSYIAGLLTGRPNSKATGPDGESLTAKEAFEKAGISTGFFDLQPKEGLALVNGTAV 265

Query: 262 GSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHI 321
           GSG+AS+VLFEAN+ AVL+E+LSAIFAEVM GKPEFTDHLTH+LKHHPGQIEAAAIMEHI
Sbjct: 266 GSGMASMVLFEANVQAVLAEVLSAIFAEVMSGKPEFTDHLTHRLKHHPGQIEAAAIMEHI 325

Query: 322 LDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVNDN 381
           LDGSSYMK A+K+HE+DPLQKPKQDRYALRTSPQWLGP IEVIR +TKSIEREINSVNDN
Sbjct: 326 LDGSSYMKLAQKVHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRQATKSIEREINSVNDN 385

Query: 382 PLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGLPSNLS 441
           PLIDVSRNKA+HGGNFQGTPIGVSMDNTRLAIA+IGKLMFAQFSELVNDFYNNGLPSNL+
Sbjct: 386 PLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNGLPSNLT 445

Query: 442 ASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAEA 501
           AS NPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKT+EA
Sbjct: 446 ASSNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTSEA 505

Query: 502 IDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCSNGALHPSRFCEK 561
           +DILKLMS+TFLV +CQA+DLRHLEENL+ TVK+TVSQVAKKVLTT  NG LHPSRFCEK
Sbjct: 506 VDILKLMSTTFLVGICQAVDLRHLEENLRQTVKNTVSQVAKKVLTTGINGELHPSRFCEK 565

Query: 562 DLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNADTSIFQKIVLFEE 621
           DLLKVVDRE  F Y+DDPC+ATYPL Q+LRQV+V+HAL+NG+ EKNA TSIFQKI  FEE
Sbjct: 566 DLLKVVDREQVFTYVDDPCSATYPLMQRLRQVIVDHALSNGETEKNAVTSIFQKIGAFEE 625

Query: 622 ELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKMLTGEKVISPGEE 681
           ELKA+LPKEVE AR AY  G + I N+IK+CRSYP+YRFVREEL TK+LTGEKV+SPGEE
Sbjct: 626 ELKAVLPKEVEAARAAYGNGTAPIPNRIKECRSYPLYRFVREELGTKLLTGEKVVSPGEE 685

Query: 682 CEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
            +KVF A+C+GK+ID +++CLKEWNGAPIPIC
Sbjct: 686 FDKVFTAMCEGKLIDPLMDCLKEWNGAPIPIC 717

BLAST of Bhi03G001224 vs. TAIR10
Match: AT2G37040.1 (PHE ammonia lyase 1)

HSP 1 Score: 1168.7 bits (3022), Expect = 0.0e+00
Identity = 580/700 (82.86%), Postives = 642/700 (91.71%), Query Frame = 0

Query: 14  ESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIATRDS 73
           +++ I+  DPLNWG AA+ +KGSHLDEVK M+ E+R+P+V +GGE+LTI QVAAI+T  +
Sbjct: 26  KNMVINAEDPLNWGAAAEQMKGSHLDEVKRMVAEFRKPVVNLGGETLTIGQVAAISTIGN 85

Query: 74  DVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFL 133
            V VELSE+ARAGV ASSDWVMESMNKGTDSYGVTTGFGATSHRRTK G ALQKELIRFL
Sbjct: 86  SVKVELSETARAGVNASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGVALQKELIRFL 145

Query: 134 NAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL 193
           NAGIFG+  E+ HTLPHSATRAAMLVRINTLLQG+SGIRFEILEAIT  LN+NITP LPL
Sbjct: 146 NAGIFGSTKETSHTLPHSATRAAMLVRINTLLQGFSGIRFEILEAITSFLNNNITPSLPL 205

Query: 194 RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQPKEGL 253
           RGTITASGDLVPLSYIAGLLTGRPNSKA GPNGE + A+EAF+ AGI SGFF+LQPKEGL
Sbjct: 206 RGTITASGDLVPLSYIAGLLTGRPNSKATGPNGEALTAEEAFKLAGISSGFFDLQPKEGL 265

Query: 254 ALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE 313
           ALVNGTAVGSG+AS+VLFE N+L+VL+EILSA+FAEVM GKPEFTDHLTH+LKHHPGQIE
Sbjct: 266 ALVNGTAVGSGMASMVLFETNVLSVLAEILSAVFAEVMSGKPEFTDHLTHRLKHHPGQIE 325

Query: 314 AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER 373
           AAAIMEHILDGSSYMK A+KLHE+DPLQKPKQDRYALRTSPQWLGP IEVIR++TKSIER
Sbjct: 326 AAAIMEHILDGSSYMKLAQKLHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRYATKSIER 385

Query: 374 EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN 433
           EINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDNTRLAIA+IGKLMFAQFSELVNDFYN
Sbjct: 386 EINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYN 445

Query: 434 NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 493
           NGLPSNL+ASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI
Sbjct: 446 NGLPSNLTASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 505

Query: 494 SSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCSNGAL 553
           SSRKT+EA+DILKLMS+TFLVA+CQA+DLRHLEENL+ TVK+TVSQVAKKVLTT  NG L
Sbjct: 506 SSRKTSEAVDILKLMSTTFLVAICQAVDLRHLEENLRQTVKNTVSQVAKKVLTTGVNGEL 565

Query: 554 HPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNADTSIF 613
           HPSRFCEKDLLKVVDRE  + Y DDPC+ATYPL QKLRQV+V+HAL NG+ EKNA TSIF
Sbjct: 566 HPSRFCEKDLLKVVDREQVYTYADDPCSATYPLIQKLRQVIVDHALINGESEKNAVTSIF 625

Query: 614 QKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKMLTGE 673
            KI  FEEELKA+LPKEVE AR AY+ G S I N+IK+CRSYP+YRFVREEL T++LTGE
Sbjct: 626 HKIGAFEEELKAVLPKEVEAARAAYDNGTSAIPNRIKECRSYPLYRFVREELGTELLTGE 685

Query: 674 KVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           KV SPGEE +KVF A+C+GK+ID ++ECL EWNGAPIPIC
Sbjct: 686 KVTSPGEEFDKVFTAICEGKIIDPMMECLNEWNGAPIPIC 725

BLAST of Bhi03G001224 vs. TAIR10
Match: AT3G10340.1 (phenylalanine ammonia-lyase 4)

HSP 1 Score: 1117.8 bits (2890), Expect = 0.0e+00
Identity = 559/693 (80.66%), Postives = 620/693 (89.47%), Query Frame = 0

Query: 22  DPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIATRDSDVIVELSE 81
           DPLNW   A++LKGSHLDEVK M++EYR+  VK+GGE+LTI QVAA+A       VEL+E
Sbjct: 16  DPLNWNATAEALKGSHLDEVKRMVKEYRKEAVKLGGETLTIGQVAAVARGGGGSTVELAE 75

Query: 82  SARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGNG 141
            ARAGVKASS+WVMESMN+GTDSYGVTTGFGATSHRRTKQGGALQ ELIRFLNAGIFG G
Sbjct: 76  EARAGVKASSEWVMESMNRGTDSYGVTTGFGATSHRRTKQGGALQNELIRFLNAGIFGPG 135

Query: 142 A-ESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPLRGTITAS 201
           A ++ HTLP   TRAAMLVR+NTLLQGYSGIRFEILEAITKLLNH ITPCLPLRGTITAS
Sbjct: 136 AGDTSHTLPKPTTRAAMLVRVNTLLQGYSGIRFEILEAITKLLNHEITPCLPLRGTITAS 195

Query: 202 GDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQPKEGLALVNGTA 261
           GDLVPLSYIAGLLTGRPNSKA+GP+GE + A EAF+ AG+ S FFELQPKEGLALVNGTA
Sbjct: 196 GDLVPLSYIAGLLTGRPNSKAVGPSGETLTASEAFKLAGV-SSFFELQPKEGLALVNGTA 255

Query: 262 VGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEH 321
           VGSGLAS VLF+ANILAVLSE++SA+FAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEH
Sbjct: 256 VGSGLASTVLFDANILAVLSEVMSAMFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEH 315

Query: 322 ILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIEREINSVND 381
           ILDGSSY+K A+ LHE+DPLQKPKQDRYALRTSPQWLGP IEVIR +TK IEREINSVND
Sbjct: 316 ILDGSSYVKEAQLLHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRAATKMIEREINSVND 375

Query: 382 NPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYNNGLPSNL 441
           NPLIDVSRNKALHGGNFQGTPIGV+MDN+RLAIASIGKLMFAQFSELVNDFYNNGLPSNL
Sbjct: 376 NPLIDVSRNKALHGGNFQGTPIGVAMDNSRLAIASIGKLMFAQFSELVNDFYNNGLPSNL 435

Query: 442 SASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTAE 501
           S  RNPSLDYGFKGAEIAMASYCSELQ+LANPVT+HVQSAEQHNQDVNSLGLISSRKTAE
Sbjct: 436 SGGRNPSLDYGFKGAEIAMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAE 495

Query: 502 AIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCSNGALHPSRFCE 561
           A+DILKLMS+T+LVALCQA+DLRHLEENLK  VKS VSQVAK+VLT  +NG LHPSRF E
Sbjct: 496 AVDILKLMSTTYLVALCQAVDLRHLEENLKKAVKSAVSQVAKRVLTVGANGELHPSRFTE 555

Query: 562 KDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNADTSIFQKIVLFE 621
           +D+L+VVDREY F+Y DDPC+ TYPL QKLR +LV+HALA+ + E N+ TS+F KI  FE
Sbjct: 556 RDVLQVVDREYVFSYADDPCSLTYPLMQKLRHILVDHALADPEREANSATSVFHKIGAFE 615

Query: 622 EELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKMLTGEKVISPGE 681
            ELK LLPKEVE  R+ YE G S I N+IK+CRSYP+YRFVR+EL T++LTGE V SPGE
Sbjct: 616 AELKLLLPKEVERVRVEYEEGTSAIANRIKECRSYPLYRFVRDELNTELLTGENVRSPGE 675

Query: 682 ECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           E +KVF+A+  GK+ID +LECLKEWNGAP+ IC
Sbjct: 676 EFDKVFLAISDGKLIDPLLECLKEWNGAPVSIC 707

BLAST of Bhi03G001224 vs. TAIR10
Match: AT5G04230.2 (phenyl alanine ammonia-lyase 3)

HSP 1 Score: 982.2 bits (2538), Expect = 1.6e-286
Identity = 510/700 (72.86%), Postives = 580/700 (82.86%), Query Frame = 0

Query: 21  NDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIATRDSDVIVELS 80
           +DPLNW VAA++LKGSHL+EVK M+++YR+  V++GGE+LTI QVAA+A+      VELS
Sbjct: 12  SDPLNWNVAAEALKGSHLEEVKKMVKDYRKGTVQLGGETLTIGQVAAVAS--GGPTVELS 71

Query: 81  ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGN 140
           E AR GVKASSDWVMESMN+ TD+YG+TTGFG++S RRT QG ALQKELIR+LNAGIF  
Sbjct: 72  EEARGGVKASSDWVMESMNRDTDTYGITTGFGSSSRRRTDQGAALQKELIRYLNAGIFAT 131

Query: 141 GAESD---HTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPLRGTI 200
           G E D   +TLP  ATRAAML+R+NTLLQGYSGIRFEILEAIT LLN  ITP LPLRGTI
Sbjct: 132 GNEDDDRSNTLPRPATRAAMLIRVNTLLQGYSGIRFEILEAITTLLNCKITPLLPLRGTI 191

Query: 201 TASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQPKEGLALVN 260
           TASGDLVPLSYIAG L GRPNS+++GP+GE + A EAF+ AG+ S FFEL+PKEGLALVN
Sbjct: 192 TASGDLVPLSYIAGFLIGRPNSRSVGPSGEILTALEAFKLAGV-SSFFELRPKEGLALVN 251

Query: 261 GTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAI 320
           GTAVGS LAS VL++ANIL V SE+ SA+FAEVMQGKPEFTDHLTHKLKHHPGQIEAAAI
Sbjct: 252 GTAVGSALASTVLYDANILVVFSEVASAMFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAI 311

Query: 321 MEHILDGSSYMKTAKKLHEIDPLQKPKQDR----YALRTSPQWLGPLIEVIRFSTKSIER 380
           MEHILDGSSY+K A  LH+IDPLQKPKQDR    YALRTSPQWLGP IEVIR +TK IER
Sbjct: 312 MEHILDGSSYVKEALHLHKIDPLQKPKQDRYVLGYALRTSPQWLGPQIEVIRAATKMIER 371

Query: 381 EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN 440
           EINSVNDNPLIDVSRNKA+HGGNFQGTPIGV+MDNTRLA+ASIGKLMFAQF+ELVNDFYN
Sbjct: 372 EINSVNDNPLIDVSRNKAIHGGNFQGTPIGVAMDNTRLALASIGKLMFAQFTELVNDFYN 431

Query: 441 NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 500
           NGLPSNLS  RNPSLDYG KGAE+AMASYCSELQ+LANPVT+HV+SA QHNQDVNSLGLI
Sbjct: 432 NGLPSNLSGGRNPSLDYGLKGAEVAMASYCSELQFLANPVTNHVESASQHNQDVNSLGLI 491

Query: 501 SSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCSNGAL 560
           SSR TAEA+ ILKLMS+T+LVALCQA DLRHLEE LK  V   VS  AK VL      A+
Sbjct: 492 SSRTTAEAVVILKLMSTTYLVALCQAFDLRHLEEILKKAVNEVVSHTAKSVL------AI 551

Query: 561 HPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNADTSIF 620
            P R    D+L VV+REY F+Y+DDP + T PL QKLR VL + ALA  + E +   ++F
Sbjct: 552 EPFR-KHDDILGVVNREYVFSYVDDPSSLTNPLMQKLRHVLFDKALAEPEGETD---TVF 611

Query: 621 QKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKMLTGE 680
           +KI  FE ELK LLPKEVE  R  YE G   + N+IK CRSYP+YRFVR ELET++LTGE
Sbjct: 612 RKIGAFEAELKFLLPKEVERVRTEYENGTFNVANRIKKCRSYPLYRFVRNELETRLLTGE 671

Query: 681 KVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
            V SPGE+ +KVF A+ QGK+ID + ECLKEWNGAPI IC
Sbjct: 672 DVRSPGEDFDKVFRAISQGKLIDPLFECLKEWNGAPISIC 698

BLAST of Bhi03G001224 vs. TrEMBL
Match: tr|A0A0A0KF39|A0A0A0KF39_CUCSA (Phenylalanine ammonia-lyase OS=Cucumis sativus OX=3659 GN=Csa_6G445760 PE=3 SV=1)

HSP 1 Score: 1340.5 bits (3468), Expect = 0.0e+00
Identity = 676/713 (94.81%), Postives = 699/713 (98.04%), Query Frame = 0

Query: 1   MAPTHFQQQNGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESL 60
           MA  HFQQ+NGS+ESLCI  NDPLNWGVAA+SLKGSHLDEVK M+EEYRRP+VK+GGE+L
Sbjct: 1   MAFAHFQQKNGSVESLCIGSNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETL 60

Query: 61  TISQVAAIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK 120
           TISQVAAIATRD+DVIVELSES+RAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK
Sbjct: 61  TISQVAAIATRDTDVIVELSESSRAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK 120

Query: 121 QGGALQKELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIT 180
           QGGALQKELIRFLNAGIFG+GAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIT
Sbjct: 121 QGGALQKELIRFLNAGIFGSGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIT 180

Query: 181 KLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGI 240
           KLLN+NITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGER+ AKEAFQQA I
Sbjct: 181 KLLNNNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERITAKEAFQQADI 240

Query: 241 PSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDH 300
           PSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDH
Sbjct: 241 PSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDH 300

Query: 301 LTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPL 360
           LTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPL
Sbjct: 301 LTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPL 360

Query: 361 IEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLM 420
           IEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLM
Sbjct: 361 IEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLM 420

Query: 421 FAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSA 480
           FAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSA
Sbjct: 421 FAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSA 480

Query: 481 EQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQV 540
           EQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVK+TVSQ+
Sbjct: 481 EQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKTTVSQI 540

Query: 541 AKKVLTTCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALA 600
           AKKVLTT SNG LHPSRFCEKDLLKVVDREYTF+YIDDPC+ATYPLSQKLRQVLVEHALA
Sbjct: 541 AKKVLTTSSNGTLHPSRFCEKDLLKVVDREYTFSYIDDPCSATYPLSQKLRQVLVEHALA 600

Query: 601 NGDDEKNADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRF 660
           NGD+EK  DTSIFQKI  FEEELKA+LPKEVEN+RLAYE+G+SKIENQIK+CRSYP+YRF
Sbjct: 601 NGDNEKTVDTSIFQKIASFEEELKAVLPKEVENSRLAYESGSSKIENQIKNCRSYPLYRF 660

Query: 661 VREELETKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           VREELETKMLTGEKVISPGEECEKVF ALCQGKMIDSILECLKEWNGAPIPIC
Sbjct: 661 VREELETKMLTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 713

BLAST of Bhi03G001224 vs. TrEMBL
Match: tr|A0A1L5JHV3|A0A1L5JHV3_LUFAE (Phenylalanine ammonia-lyase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 1328.2 bits (3436), Expect = 0.0e+00
Identity = 669/700 (95.57%), Postives = 690/700 (98.57%), Query Frame = 0

Query: 14  ESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIATRDS 73
           +SLC S+NDPLNWGVAA+SLKGSHLDEVK M+EEYRRP+VK+GGE+LTISQVAAIATRDS
Sbjct: 9   DSLCHSRNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDS 68

Query: 74  DVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFL 133
           DV VELSESARAGVKASSDWVMESMNKGT+SYGVTTGFGATSHRRTKQGGALQKELIRFL
Sbjct: 69  DVTVELSESARAGVKASSDWVMESMNKGTESYGVTTGFGATSHRRTKQGGALQKELIRFL 128

Query: 134 NAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL 193
           NAGIFG+GAES+HTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL
Sbjct: 129 NAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL 188

Query: 194 RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQPKEGL 253
           RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGE +DAKEAFQQAGIPSGFFELQPKEGL
Sbjct: 189 RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAGIPSGFFELQPKEGL 248

Query: 254 ALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE 313
           ALVNGTAVGSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE
Sbjct: 249 ALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE 308

Query: 314 AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER 373
           AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER
Sbjct: 309 AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER 368

Query: 374 EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN 433
           EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN
Sbjct: 369 EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN 428

Query: 434 NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 493
           NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI
Sbjct: 429 NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 488

Query: 494 SSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCSNGAL 553
           SSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQ+AKKVLTT SNGAL
Sbjct: 489 SSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQIAKKVLTTSSNGAL 548

Query: 554 HPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNADTSIF 613
           HPSRFCEKDLLKVVDREYTFAYIDDPC+ATYPLSQKLRQVLVEHALANG+ EKN DTSIF
Sbjct: 549 HPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHALANGEGEKNTDTSIF 608

Query: 614 QKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKMLTGE 673
           QKI+ FEEELKA+LPKEVENARL+YE+GNSKIENQIKDCRSYP+YRFVREEL TK+LTGE
Sbjct: 609 QKIITFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYRFVREELGTKLLTGE 668

Query: 674 KVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           KVISPGEECEKVF ALCQGKMIDSILECLKEWNGAPIPIC
Sbjct: 669 KVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 708

BLAST of Bhi03G001224 vs. TrEMBL
Match: tr|A0A1S3BZ45|A0A1S3BZ45_CUCME (Phenylalanine ammonia-lyase OS=Cucumis melo OX=3656 GN=LOC103494629 PE=3 SV=1)

HSP 1 Score: 1298.9 bits (3360), Expect = 0.0e+00
Identity = 654/713 (91.73%), Postives = 682/713 (95.65%), Query Frame = 0

Query: 1   MAPTHFQQQNGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESL 60
           MA      QNGS+ESLC S  DPLNWG+AADS+KGSHLDEVK M+EEYRRP+VK+GGE+L
Sbjct: 1   MAQNVCANQNGSVESLCNSHQDPLNWGLAADSMKGSHLDEVKRMVEEYRRPLVKLGGETL 60

Query: 61  TISQVAAIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK 120
           TISQVAAIATRD+DV+VEL+ESARAGVKASSDWVMESM KGTDSYGVTTGFGATSHRRT 
Sbjct: 61  TISQVAAIATRDNDVVVELAESARAGVKASSDWVMESMGKGTDSYGVTTGFGATSHRRTN 120

Query: 121 QGGALQKELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIT 180
           QGGALQKELIRFLN+GIFGNG+ES+HTLPHSATRAAMLVRINTLLQGYSGIRFEILEAI 
Sbjct: 121 QGGALQKELIRFLNSGIFGNGSESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIA 180

Query: 181 KLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGI 240
            LLNHN+TPCLPLRGTITASGDLVPLSYIAGLLTGR NSKAIGPNGE +DAK AF+QAGI
Sbjct: 181 NLLNHNVTPCLPLRGTITASGDLVPLSYIAGLLTGRHNSKAIGPNGETLDAKAAFEQAGI 240

Query: 241 PSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDH 300
            SGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDH
Sbjct: 241 DSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDH 300

Query: 301 LTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPL 360
           LTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHE+DPLQKPKQDRYALRTSPQWLGPL
Sbjct: 301 LTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEMDPLQKPKQDRYALRTSPQWLGPL 360

Query: 361 IEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLM 420
           IEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLA+ASIGKLM
Sbjct: 361 IEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAVASIGKLM 420

Query: 421 FAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSA 480
           FAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSA
Sbjct: 421 FAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSA 480

Query: 481 EQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQV 540
           EQHNQDVNSLGLISSRKT EAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVK+TVSQV
Sbjct: 481 EQHNQDVNSLGLISSRKTVEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKNTVSQV 540

Query: 541 AKKVLTTCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALA 600
           AKKVLTT SNGALHPSRFCEKDLLKVVDREYTFAYIDDPC+ATYPL QKLRQ+LVEHAL 
Sbjct: 541 AKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLMQKLRQILVEHALK 600

Query: 601 NGDDEKNADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRF 660
           NGD+E NA+TSIFQKI  FEEELKA+LPKEVEN RLAYE GNSKI+NQIKDCRSYP+Y+F
Sbjct: 601 NGDNETNANTSIFQKIGAFEEELKAILPKEVENVRLAYENGNSKIKNQIKDCRSYPLYKF 660

Query: 661 VREELETKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           VREEL TK+LTGEKVISPGEECEKVF AL QGKMIDSI ECLKEWNGAPIPIC
Sbjct: 661 VREELGTKLLTGEKVISPGEECEKVFTALSQGKMIDSIFECLKEWNGAPIPIC 713

BLAST of Bhi03G001224 vs. TrEMBL
Match: tr|A0A1S3BKY9|A0A1S3BKY9_CUCME (Phenylalanine ammonia-lyase OS=Cucumis melo OX=3656 GN=LOC103491172 PE=3 SV=1)

HSP 1 Score: 1298.5 bits (3359), Expect = 0.0e+00
Identity = 654/717 (91.21%), Postives = 685/717 (95.54%), Query Frame = 0

Query: 1   MAPTHFQQ---QNGSMESLCISQ-NDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIG 60
           MAP   +Q   QNGSMES C S   DPLNWGVAA++LKGSHLDEVK M++EYRRP+VK+G
Sbjct: 1   MAPAQLEQFAHQNGSMESFCTSHVVDPLNWGVAAEALKGSHLDEVKRMVDEYRRPVVKLG 60

Query: 61  GESLTISQVAAIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSH 120
           GE+LTISQVAAIATRDSDV+V+L+ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSH
Sbjct: 61  GETLTISQVAAIATRDSDVVVKLAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSH 120

Query: 121 RRTKQGGALQKELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEIL 180
           RRTKQGGALQKELIRFLNAGIFGNGAES+HTLPHSATRAAMLVRINTLLQGYSGIRFEIL
Sbjct: 121 RRTKQGGALQKELIRFLNAGIFGNGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEIL 180

Query: 181 EAITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQ 240
           EAIT LLN N+TPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGE +DA+ AF+
Sbjct: 181 EAITNLLNENVTPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGETLDAEAAFK 240

Query: 241 QAGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPE 300
           QAGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPE
Sbjct: 241 QAGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPE 300

Query: 301 FTDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQW 360
           +TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMK AKKLHEIDPLQKPKQDRYALRTSPQW
Sbjct: 301 YTDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQW 360

Query: 361 LGPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASI 420
           LGPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASI
Sbjct: 361 LGPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASI 420

Query: 421 GKLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSH 480
           GKLMFAQFSELVNDFYNNGLPSNLS SRNPSLDYGFKGAEIAMASYCSELQYLANPVTSH
Sbjct: 421 GKLMFAQFSELVNDFYNNGLPSNLSGSRNPSLDYGFKGAEIAMASYCSELQYLANPVTSH 480

Query: 481 VQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKST 540
           VQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKS 
Sbjct: 481 VQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSI 540

Query: 541 VSQVAKKVLTTCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVE 600
           VSQVAKKVLTT SNG LHPSRFCEKDLLKVVDREYTFAYIDDPC+ATYPL QKLRQVLV+
Sbjct: 541 VSQVAKKVLTTSSNGTLHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLMQKLRQVLVD 600

Query: 601 HALANGDDEKNADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYP 660
           HAL NG++EKN +TSIFQKI  FEEELK  LPKEVENARLAYE+GN K+ NQIKDCRSYP
Sbjct: 601 HALTNGENEKNTNTSIFQKITAFEEELKVALPKEVENARLAYESGNCKVANQIKDCRSYP 660

Query: 661 IYRFVREELETKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           +Y+FVRE+L TK+LTGE+VISPGEECEKVF ALCQGKMIDSI+ECLKEWNGAPIPIC
Sbjct: 661 LYKFVREDLGTKLLTGERVISPGEECEKVFTALCQGKMIDSIMECLKEWNGAPIPIC 717

BLAST of Bhi03G001224 vs. TrEMBL
Match: tr|A0A0A0KH72|A0A0A0KH72_CUCSA (Phenylalanine ammonia-lyase OS=Cucumis sativus OX=3659 GN=Csa_6G445770 PE=3 SV=1)

HSP 1 Score: 1291.9 bits (3342), Expect = 0.0e+00
Identity = 653/717 (91.07%), Postives = 682/717 (95.12%), Query Frame = 0

Query: 1   MAPTHFQQ---QNGSMESLCISQ-NDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIG 60
           MAP   +Q   QNGSMES C S   DPLNWGVAA++LKGSHLDEVK M++EYRRP+VK+G
Sbjct: 1   MAPAQLEQFAHQNGSMESFCTSHVVDPLNWGVAAEALKGSHLDEVKRMVDEYRRPVVKLG 60

Query: 61  GESLTISQVAAIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSH 120
           GE+LTISQVAAIATRD+DV+V+L+ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSH
Sbjct: 61  GETLTISQVAAIATRDNDVVVKLAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSH 120

Query: 121 RRTKQGGALQKELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEIL 180
           RRTKQGGALQKELIRFLNAGIFGNGAES+HTLPHSATRAAMLVRINTLLQGYSGIRFEIL
Sbjct: 121 RRTKQGGALQKELIRFLNAGIFGNGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEIL 180

Query: 181 EAITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQ 240
           EAIT LLN N+TPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGE +DA+ AF+
Sbjct: 181 EAITNLLNENVTPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGETLDAEAAFK 240

Query: 241 QAGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPE 300
           QAGIPS FFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPE
Sbjct: 241 QAGIPSSFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPE 300

Query: 301 FTDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQW 360
           FTDHLTHKLKHHPGQIEAAAIMEHILDGSSYMK AKKLHEIDPLQKPKQDRYALRTSPQW
Sbjct: 301 FTDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKAAKKLHEIDPLQKPKQDRYALRTSPQW 360

Query: 361 LGPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASI 420
           LGPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASI
Sbjct: 361 LGPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASI 420

Query: 421 GKLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSH 480
           GKLMFAQFSEL NDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSH
Sbjct: 421 GKLMFAQFSELFNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSH 480

Query: 481 VQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKST 540
           VQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKS 
Sbjct: 481 VQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSV 540

Query: 541 VSQVAKKVLTTCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVE 600
           VSQVAKKVLTT S G LHPSRFCEKDLLKVVDREYTFAYIDDPC+ATYPL QKLRQVLV+
Sbjct: 541 VSQVAKKVLTTSSIGTLHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLMQKLRQVLVD 600

Query: 601 HALANGDDEKNADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYP 660
           HAL NG++EKN +TSIFQKI  FEEELK  LPKEVEN RLAYE+GNSK+ NQIKDCRSYP
Sbjct: 601 HALTNGENEKNMNTSIFQKITAFEEELKVALPKEVENTRLAYESGNSKVANQIKDCRSYP 660

Query: 661 IYRFVREELETKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           +Y+FVRE+L  K+LTGE+VISPGEECEKVF ALCQGKMIDSILECLKEWNGAPIPIC
Sbjct: 661 LYKFVREDLGAKLLTGERVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 717

BLAST of Bhi03G001224 vs. NCBI nr
Match: XP_004143258.1 (PREDICTED: phenylalanine ammonia-lyase-like [Cucumis sativus] >KGN48163.1 hypothetical protein Csa_6G445760 [Cucumis sativus])

HSP 1 Score: 1340.5 bits (3468), Expect = 0.0e+00
Identity = 676/713 (94.81%), Postives = 699/713 (98.04%), Query Frame = 0

Query: 1   MAPTHFQQQNGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESL 60
           MA  HFQQ+NGS+ESLCI  NDPLNWGVAA+SLKGSHLDEVK M+EEYRRP+VK+GGE+L
Sbjct: 1   MAFAHFQQKNGSVESLCIGSNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETL 60

Query: 61  TISQVAAIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK 120
           TISQVAAIATRD+DVIVELSES+RAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK
Sbjct: 61  TISQVAAIATRDTDVIVELSESSRAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTK 120

Query: 121 QGGALQKELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIT 180
           QGGALQKELIRFLNAGIFG+GAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIT
Sbjct: 121 QGGALQKELIRFLNAGIFGSGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAIT 180

Query: 181 KLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGI 240
           KLLN+NITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGER+ AKEAFQQA I
Sbjct: 181 KLLNNNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERITAKEAFQQADI 240

Query: 241 PSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDH 300
           PSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDH
Sbjct: 241 PSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDH 300

Query: 301 LTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPL 360
           LTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPL
Sbjct: 301 LTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPL 360

Query: 361 IEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLM 420
           IEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLM
Sbjct: 361 IEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLM 420

Query: 421 FAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSA 480
           FAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSA
Sbjct: 421 FAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSA 480

Query: 481 EQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQV 540
           EQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVK+TVSQ+
Sbjct: 481 EQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKTTVSQI 540

Query: 541 AKKVLTTCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALA 600
           AKKVLTT SNG LHPSRFCEKDLLKVVDREYTF+YIDDPC+ATYPLSQKLRQVLVEHALA
Sbjct: 541 AKKVLTTSSNGTLHPSRFCEKDLLKVVDREYTFSYIDDPCSATYPLSQKLRQVLVEHALA 600

Query: 601 NGDDEKNADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRF 660
           NGD+EK  DTSIFQKI  FEEELKA+LPKEVEN+RLAYE+G+SKIENQIK+CRSYP+YRF
Sbjct: 601 NGDNEKTVDTSIFQKIASFEEELKAVLPKEVENSRLAYESGSSKIENQIKNCRSYPLYRF 660

Query: 661 VREELETKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           VREELETKMLTGEKVISPGEECEKVF ALCQGKMIDSILECLKEWNGAPIPIC
Sbjct: 661 VREELETKMLTGEKVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 713

BLAST of Bhi03G001224 vs. NCBI nr
Match: APO14269.1 (phenylalanine ammonia-lyase 3 [Luffa aegyptiaca])

HSP 1 Score: 1328.2 bits (3436), Expect = 0.0e+00
Identity = 669/700 (95.57%), Postives = 690/700 (98.57%), Query Frame = 0

Query: 14  ESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGGESLTISQVAAIATRDS 73
           +SLC S+NDPLNWGVAA+SLKGSHLDEVK M+EEYRRP+VK+GGE+LTISQVAAIATRDS
Sbjct: 9   DSLCHSRNDPLNWGVAAESLKGSHLDEVKRMVEEYRRPLVKLGGETLTISQVAAIATRDS 68

Query: 74  DVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFL 133
           DV VELSESARAGVKASSDWVMESMNKGT+SYGVTTGFGATSHRRTKQGGALQKELIRFL
Sbjct: 69  DVTVELSESARAGVKASSDWVMESMNKGTESYGVTTGFGATSHRRTKQGGALQKELIRFL 128

Query: 134 NAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL 193
           NAGIFG+GAES+HTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL
Sbjct: 129 NAGIFGSGAESNHTLPHSATRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNITPCLPL 188

Query: 194 RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQAGIPSGFFELQPKEGL 253
           RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGE +DAKEAFQQAGIPSGFFELQPKEGL
Sbjct: 189 RGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGESIDAKEAFQQAGIPSGFFELQPKEGL 248

Query: 254 ALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE 313
           ALVNGTAVGSGLASIVLFEANILAVLSE+LSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE
Sbjct: 249 ALVNGTAVGSGLASIVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIE 308

Query: 314 AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER 373
           AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER
Sbjct: 309 AAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWLGPLIEVIRFSTKSIER 368

Query: 374 EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN 433
           EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN
Sbjct: 369 EINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIGKLMFAQFSELVNDFYN 428

Query: 434 NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 493
           NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI
Sbjct: 429 NGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLI 488

Query: 494 SSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQVAKKVLTTCSNGAL 553
           SSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQ+AKKVLTT SNGAL
Sbjct: 489 SSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTVSQIAKKVLTTSSNGAL 548

Query: 554 HPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEHALANGDDEKNADTSIF 613
           HPSRFCEKDLLKVVDREYTFAYIDDPC+ATYPLSQKLRQVLVEHALANG+ EKN DTSIF
Sbjct: 549 HPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEHALANGEGEKNTDTSIF 608

Query: 614 QKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPIYRFVREELETKMLTGE 673
           QKI+ FEEELKA+LPKEVENARL+YE+GNSKIENQIKDCRSYP+YRFVREEL TK+LTGE
Sbjct: 609 QKIITFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPLYRFVREELGTKLLTGE 668

Query: 674 KVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           KVISPGEECEKVF ALCQGKMIDSILECLKEWNGAPIPIC
Sbjct: 669 KVISPGEECEKVFTALCQGKMIDSILECLKEWNGAPIPIC 708

BLAST of Bhi03G001224 vs. NCBI nr
Match: XP_023518835.1 (phenylalanine ammonia-lyase-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1320.4 bits (3416), Expect = 0.0e+00
Identity = 667/716 (93.16%), Postives = 691/716 (96.51%), Query Frame = 0

Query: 1   MAPTHFQQ---QNGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGG 60
           MAP  FQQ   QNGS+E+ CIS NDPLNWGVAAD+LKGSHLDEVK M+EEYRRP+VK+GG
Sbjct: 1   MAPAPFQQLAAQNGSVETFCISHNDPLNWGVAADALKGSHLDEVKRMVEEYRRPLVKLGG 60

Query: 61  ESLTISQVAAIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120
           E+LTISQVAAIATRD+DVIVEL+ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR
Sbjct: 61  ETLTISQVAAIATRDNDVIVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120

Query: 121 RTKQGGALQKELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180
           RT QGGALQKELIRFLNAGIFG+GAES HTLPHSATRAAMLVRINTLLQGYSGIRFEILE
Sbjct: 121 RTNQGGALQKELIRFLNAGIFGSGAESTHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180

Query: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQ 240
           AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA+GPNGE +DA EAFQQ
Sbjct: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPNGESLDAMEAFQQ 240

Query: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300
           AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF
Sbjct: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300

Query: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL 360
           TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHE DPLQKPKQDRYALRTSPQWL
Sbjct: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHETDPLQKPKQDRYALRTSPQWL 360

Query: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG 420
           GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDNTRLA+ASIG
Sbjct: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAVASIG 420

Query: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480
           KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV
Sbjct: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480

Query: 481 QSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTV 540
           QSAEQHNQDVNSLGLISSRKTAEA+DILKLMSSTFLVALCQAIDLRHLEENLK+TVK+ V
Sbjct: 481 QSAEQHNQDVNSLGLISSRKTAEALDILKLMSSTFLVALCQAIDLRHLEENLKNTVKNCV 540

Query: 541 SQVAKKVLTTCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEH 600
           SQ AKKVL T SNGALHPSRFCEKDLLKVVDREYTFAYIDDPC+ATYPLSQKLRQVLVEH
Sbjct: 541 SQTAKKVLITSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEH 600

Query: 601 ALANGDDEKNADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPI 660
           AL NGD EK+ADTSIFQKI  FEEELKA+LPKEVENARL YE+GNSKIENQIKDCRSYP+
Sbjct: 601 ALGNGDCEKDADTSIFQKIGAFEEELKAVLPKEVENARLWYESGNSKIENQIKDCRSYPL 660

Query: 661 YRFVREELETKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           YRFVREEL T +LTGEKVISPGEECEKVF ALC+GKMIDSILECLKEWNGAPIPIC
Sbjct: 661 YRFVREELGTNLLTGEKVISPGEECEKVFTALCEGKMIDSILECLKEWNGAPIPIC 716

BLAST of Bhi03G001224 vs. NCBI nr
Match: XP_023518834.1 (phenylalanine ammonia-lyase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1318.1 bits (3410), Expect = 0.0e+00
Identity = 665/716 (92.88%), Postives = 691/716 (96.51%), Query Frame = 0

Query: 1   MAPTHFQQ---QNGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGG 60
           MAP  FQQ   QNGS+E+ CIS NDPLNWGVAAD+LKGSHLDEVK M+EEYRRP+VK+GG
Sbjct: 1   MAPAPFQQLAAQNGSVETFCISHNDPLNWGVAADALKGSHLDEVKRMVEEYRRPLVKLGG 60

Query: 61  ESLTISQVAAIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120
           E+LTISQVAAIATRD+DVIVEL+ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR
Sbjct: 61  ETLTISQVAAIATRDNDVIVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120

Query: 121 RTKQGGALQKELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180
           RT QGGALQKELIRFLNAGIFG+GAES HTLPHSATRAAMLVRINTLLQGYSGIRFEILE
Sbjct: 121 RTNQGGALQKELIRFLNAGIFGSGAESTHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180

Query: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQ 240
           AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA+GPNGE +DA EAFQQ
Sbjct: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPNGESLDAMEAFQQ 240

Query: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300
           AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF
Sbjct: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300

Query: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL 360
           TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHE DPLQKPKQDRYALRTSPQWL
Sbjct: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHETDPLQKPKQDRYALRTSPQWL 360

Query: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG 420
           GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDNTRLA+ASIG
Sbjct: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAVASIG 420

Query: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480
           KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV
Sbjct: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480

Query: 481 QSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTV 540
           QSAEQHNQDVNSLGLISSRKTAEA+DILKLMSSTFLVALCQAIDLRHLEENLK+TVK+ V
Sbjct: 481 QSAEQHNQDVNSLGLISSRKTAEALDILKLMSSTFLVALCQAIDLRHLEENLKNTVKNCV 540

Query: 541 SQVAKKVLTTCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEH 600
           SQ AKKVL T SNGALHPSRFCEKDLLKVVDREYTFAYIDDPC+ATYPLSQKLRQVLVEH
Sbjct: 541 SQTAKKVLITSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEH 600

Query: 601 ALANGDDEKNADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPI 660
           AL NGD EK+ADTSIFQKI  FEEELKA+LPKEVENARL+Y +GNSKIENQIKDCRSYP+
Sbjct: 601 ALGNGDCEKDADTSIFQKIGAFEEELKAVLPKEVENARLSYGSGNSKIENQIKDCRSYPL 660

Query: 661 YRFVREELETKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           YRFVREEL T ++TGEKVISPGEECEKVF ALC+GKMIDSILECLKEWNGAPIPIC
Sbjct: 661 YRFVREELGTNLVTGEKVISPGEECEKVFTALCEGKMIDSILECLKEWNGAPIPIC 716

BLAST of Bhi03G001224 vs. NCBI nr
Match: XP_023518836.1 (phenylalanine ammonia-lyase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1314.7 bits (3401), Expect = 0.0e+00
Identity = 662/716 (92.46%), Postives = 691/716 (96.51%), Query Frame = 0

Query: 1   MAPTHFQQ---QNGSMESLCISQNDPLNWGVAADSLKGSHLDEVKHMIEEYRRPIVKIGG 60
           MAP  FQQ   QNGS+ES CIS+NDPLNWGVAA++LKGSHLDEVK M+EEYRRP+VK+GG
Sbjct: 1   MAPAPFQQLAAQNGSVESFCISRNDPLNWGVAAEALKGSHLDEVKRMVEEYRRPLVKLGG 60

Query: 61  ESLTISQVAAIATRDSDVIVELSESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120
           ++LTISQVAAIATRD+DV+VEL+ESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR
Sbjct: 61  QTLTISQVAAIATRDNDVMVELAESARAGVKASSDWVMESMNKGTDSYGVTTGFGATSHR 120

Query: 121 RTKQGGALQKELIRFLNAGIFGNGAESDHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180
           RT QGGALQKELIRFLNAGIFG+GAES HTLPHSATRAAMLVRINTLLQGYSGIRFEILE
Sbjct: 121 RTNQGGALQKELIRFLNAGIFGSGAESTHTLPHSATRAAMLVRINTLLQGYSGIRFEILE 180

Query: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAIGPNGERMDAKEAFQQ 240
           AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKA+GPNGE +DAKEAFQQ
Sbjct: 181 AITKLLNHNITPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPNGESLDAKEAFQQ 240

Query: 241 AGIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANILAVLSEILSAIFAEVMQGKPEF 300
           A IPSGFFELQPKEGLALVNGTAVGSGLASIVLFEAN LAVLSEILSAIFAEVMQGKPEF
Sbjct: 241 ADIPSGFFELQPKEGLALVNGTAVGSGLASIVLFEANTLAVLSEILSAIFAEVMQGKPEF 300

Query: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKTAKKLHEIDPLQKPKQDRYALRTSPQWL 360
           TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMK AKKLHE DPLQKPKQDRYALRTSPQWL
Sbjct: 301 TDHLTHKLKHHPGQIEAAAIMEHILDGSSYMKAAKKLHETDPLQKPKQDRYALRTSPQWL 360

Query: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIASIG 420
           GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKA+HGGNFQGTPIGVSMDNTRLA+ASIG
Sbjct: 361 GPLIEVIRFSTKSIEREINSVNDNPLIDVSRNKAIHGGNFQGTPIGVSMDNTRLAVASIG 420

Query: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480
           KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV
Sbjct: 421 KLMFAQFSELVNDFYNNGLPSNLSASRNPSLDYGFKGAEIAMASYCSELQYLANPVTSHV 480

Query: 481 QSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTFLVALCQAIDLRHLEENLKSTVKSTV 540
           QSAEQHNQDVNSLGLISSRKTAEA+DILKLMSSTFLVALCQAIDLRHLEENLK+TVK+ V
Sbjct: 481 QSAEQHNQDVNSLGLISSRKTAEALDILKLMSSTFLVALCQAIDLRHLEENLKNTVKNCV 540

Query: 541 SQVAKKVLTTCSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCNATYPLSQKLRQVLVEH 600
           SQ AKKVLTT SNGALHPSRFCEKDLLKVVDREYTFAYIDDPC+ATYPLSQKLRQVLVEH
Sbjct: 541 SQTAKKVLTTSSNGALHPSRFCEKDLLKVVDREYTFAYIDDPCSATYPLSQKLRQVLVEH 600

Query: 601 ALANGDDEKNADTSIFQKIVLFEEELKALLPKEVENARLAYETGNSKIENQIKDCRSYPI 660
           AL NGD EK+AD+SIFQKI  FEEELKA+LPKEVENARL+YE+GNSKIENQIKDCRSYP+
Sbjct: 601 ALGNGDCEKDADSSIFQKIGAFEEELKAVLPKEVENARLSYESGNSKIENQIKDCRSYPL 660

Query: 661 YRFVREELETKMLTGEKVISPGEECEKVFIALCQGKMIDSILECLKEWNGAPIPIC 714
           YRFVREEL T +LTGEKVISPGE CEKVF ALC+GKMIDSILECLKEWNGAPIPIC
Sbjct: 661 YRFVREELRTNLLTGEKVISPGEACEKVFTALCEGKMIDSILECLKEWNGAPIPIC 716

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|P45730|PALY_POPTR0.0e+0085.69Phenylalanine ammonia-lyase OS=Populus trichocarpa OX=3694 GN=PAL PE=2 SV=1[more]
sp|P45732|PALY_STYHU0.0e+0086.17Phenylalanine ammonia-lyase OS=Stylosanthes humilis OX=35628 GN=PAL17.1 PE=2 SV=... [more]
sp|P45734|PALY_TRISU0.0e+0085.37Phenylalanine ammonia-lyase OS=Trifolium subterraneum OX=3900 GN=PAL1 PE=3 SV=1[more]
sp|Q04593|PAL2_PEA0.0e+0084.92Phenylalanine ammonia-lyase 2 OS=Pisum sativum OX=3888 GN=PAL2 PE=2 SV=1[more]
sp|O64963|PAL1_PRUAV0.0e+0084.87Phenylalanine ammonia-lyase 1 OS=Prunus avium OX=42229 GN=PAL1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G53260.10.0e+0083.82phenylalanine ammonia-lyase 2[more]
AT2G37040.10.0e+0082.86PHE ammonia lyase 1[more]
AT3G10340.10.0e+0080.66phenylalanine ammonia-lyase 4[more]
AT5G04230.21.6e-28672.86phenyl alanine ammonia-lyase 3[more]
Match NameE-valueIdentityDescription
tr|A0A0A0KF39|A0A0A0KF39_CUCSA0.0e+0094.81Phenylalanine ammonia-lyase OS=Cucumis sativus OX=3659 GN=Csa_6G445760 PE=3 SV=1[more]
tr|A0A1L5JHV3|A0A1L5JHV3_LUFAE0.0e+0095.57Phenylalanine ammonia-lyase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
tr|A0A1S3BZ45|A0A1S3BZ45_CUCME0.0e+0091.73Phenylalanine ammonia-lyase OS=Cucumis melo OX=3656 GN=LOC103494629 PE=3 SV=1[more]
tr|A0A1S3BKY9|A0A1S3BKY9_CUCME0.0e+0091.21Phenylalanine ammonia-lyase OS=Cucumis melo OX=3656 GN=LOC103491172 PE=3 SV=1[more]
tr|A0A0A0KH72|A0A0A0KH72_CUCSA0.0e+0091.07Phenylalanine ammonia-lyase OS=Cucumis sativus OX=3659 GN=Csa_6G445770 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004143258.10.0e+0094.81PREDICTED: phenylalanine ammonia-lyase-like [Cucumis sativus] >KGN48163.1 hypoth... [more]
APO14269.10.0e+0095.57phenylalanine ammonia-lyase 3 [Luffa aegyptiaca][more]
XP_023518835.10.0e+0093.16phenylalanine ammonia-lyase-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023518834.10.0e+0092.88phenylalanine ammonia-lyase-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023518836.10.0e+0092.46phenylalanine ammonia-lyase-like [Cucurbita pepo subsp. pepo][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0016841ammonia-lyase activity
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
Vocabulary: Biological Process
TermDefinition
GO:0006559L-phenylalanine catabolic process
Vocabulary: INTERPRO
TermDefinition
IPR008948L-Aspartase-like
IPR022313Phe/His_NH3-lyase_AS
IPR001106Aromatic_Lyase
IPR005922Phe_NH3-lyase
IPR024083Fumarase/histidase_N
IPR023144Phe_NH3-lyase_shielding_dom_sf
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009800 cinnamic acid biosynthetic process
biological_process GO:0006559 L-phenylalanine catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0045548 phenylalanine ammonia-lyase activity
molecular_function GO:0016841 ammonia-lyase activity
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi03M001224Bhi03M001224mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023144Phenylalanine ammonia-lyase, shielding domain superfamilyGENE3DG3DSA:1.10.274.20coord: 526..640
e-value: 5.0E-216
score: 719.4
NoneNo IPR availableGENE3DG3DSA:1.20.200.10coord: 260..525
e-value: 5.0E-216
score: 719.4
coord: 647..702
e-value: 5.0E-216
score: 719.4
NoneNo IPR availablePANTHERPTHR10362:SF32SUBFAMILY NOT NAMEDcoord: 10..713
IPR024083Fumarase/histidase, N-terminalGENE3DG3DSA:1.10.275.10coord: 21..259
e-value: 8.6E-98
score: 328.4
IPR005922Phenylalanine ammonia-lyaseTIGRFAMTIGR01226TIGR01226coord: 22..703
e-value: 0.0
score: 1159.0
IPR001106Aromatic amino acid lyasePFAMPF00221Lyase_aromaticcoord: 60..533
e-value: 2.0E-155
score: 517.9
IPR001106Aromatic amino acid lyasePANTHERPTHR10362HISTIDINE AMMONIA-LYASEcoord: 10..713
IPR001106Aromatic amino acid lyaseCDDcd00332PAL-HALcoord: 58..523
e-value: 0.0
score: 573.307
IPR022313Phenylalanine/histidine ammonia-lyases, active sitePROSITEPS00488PAL_HISTIDASEcoord: 195..211
IPR008948L-Aspartase-likeSUPERFAMILYSSF48557L-aspartase-likecoord: 22..713

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Bhi03G001224Wax gourdwgowgoB008
Bhi03G001224Cucumber (Gy14) v1cgywgoB227
Bhi03G001224Cucumber (Gy14) v1cgywgoB614
Bhi03G001224Cucumber (Gy14) v1cgywgoB772
Bhi03G001224Cucumber (Gy14) v2cgybwgoB320
Bhi03G001224Cucumber (Gy14) v2cgybwgoB599
Bhi03G001224Cucumber (Gy14) v2cgybwgoB603
Bhi03G001224Cucurbita maxima (Rimu)cmawgoB0355
Bhi03G001224Cucurbita maxima (Rimu)cmawgoB0791
Bhi03G001224Cucurbita maxima (Rimu)cmawgoB0881
Bhi03G001224Cucurbita maxima (Rimu)cmawgoB1037
Bhi03G001224Cucurbita maxima (Rimu)cmawgoB1061
Bhi03G001224Cucurbita moschata (Rifu)cmowgoB0345
Bhi03G001224Cucurbita moschata (Rifu)cmowgoB0696
Bhi03G001224Cucurbita moschata (Rifu)cmowgoB0783
Bhi03G001224Cucurbita moschata (Rifu)cmowgoB0878
Bhi03G001224Cucurbita moschata (Rifu)cmowgoB1031
Bhi03G001224Cucurbita moschata (Rifu)cmowgoB1054
Bhi03G001224Cucurbita pepo (Zucchini)cpewgoB0100
Bhi03G001224Cucurbita pepo (Zucchini)cpewgoB0368
Bhi03G001224Cucurbita pepo (Zucchini)cpewgoB0545
Bhi03G001224Cucurbita pepo (Zucchini)cpewgoB0610
Bhi03G001224Cucurbita pepo (Zucchini)cpewgoB0695
Bhi03G001224Cucurbita pepo (Zucchini)cpewgoB0811
Bhi03G001224Cucurbita pepo (Zucchini)cpewgoB1131
Bhi03G001224Wild cucumber (PI 183967)cpiwgoB332
Bhi03G001224Wild cucumber (PI 183967)cpiwgoB635
Bhi03G001224Wild cucumber (PI 183967)cpiwgoB639
Bhi03G001224Cucumber (Chinese Long) v3cucwgoB336
Bhi03G001224Cucumber (Chinese Long) v3cucwgoB649
Bhi03G001224Cucumber (Chinese Long) v3cucwgoB653
Bhi03G001224Cucumber (Chinese Long) v2cuwgoB329
Bhi03G001224Cucumber (Chinese Long) v2cuwgoB629
Bhi03G001224Cucumber (Chinese Long) v2cuwgoB633
Bhi03G001224Bottle gourd (USVL1VR-Ls)lsiwgoB087
Bhi03G001224Bottle gourd (USVL1VR-Ls)lsiwgoB170
Bhi03G001224Bottle gourd (USVL1VR-Ls)lsiwgoB332
Bhi03G001224Melon (DHL92) v3.6.1medwgoB144
Bhi03G001224Melon (DHL92) v3.6.1medwgoB423
Bhi03G001224Melon (DHL92) v3.6.1medwgoB651
Bhi03G001224Melon (DHL92) v3.5.1mewgoB421
Bhi03G001224Melon (DHL92) v3.5.1mewgoB645
Bhi03G001224Watermelon (Charleston Gray)wcgwgoB122
Bhi03G001224Watermelon (Charleston Gray)wcgwgoB313
Bhi03G001224Watermelon (Charleston Gray)wcgwgoB391
Bhi03G001224Watermelon (97103) v2wgowmbB408
Bhi03G001224Watermelon (97103) v2wgowmbB418
Bhi03G001224Watermelon (97103) v2wgowmbB432
Bhi03G001224Watermelon (97103) v1wgowmB409
Bhi03G001224Watermelon (97103) v1wgowmB450
Bhi03G001224Watermelon (97103) v1wgowmB459
Bhi03G001224Silver-seed gourdcarwgoB0209
Bhi03G001224Silver-seed gourdcarwgoB0306
Bhi03G001224Silver-seed gourdcarwgoB0329
Bhi03G001224Silver-seed gourdcarwgoB0423